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[PMID]:28576653
[Au] Autor:Cêtre-Sossah C; Dickmu S; Kwiatek O; Albina E
[Ad] Endereço:CIRAD UMR ASTRE, F-97490 Sainte Clotilde, France; INRA, UMR1309 CMAEE, F-34398 Montpellier, France. Electronic address: catherine.cetre-sossah@cirad.fr.
[Ti] Título:A G-protein-coupled chemokine receptor: A putative insertion site for a multi-pathogen recombinant capripoxvirus vaccine strategy.
[So] Source:J Immunol Methods;448:112-115, 2017 Sep.
[Is] ISSN:1872-7905
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Capripoxviruses (CaPVs) have been shown to be ideal viral vectors for the development of recombinant multivalent vaccines to enable delivery of immunogenic genes from ruminant pathogens. So far, the viral thymidine kinase (TK) gene is the only gene used to generate recombinants. A putative non-essential gene encoding a G-protein-coupled chemokine receptor subfamily homologue (GPCR) was targeted as an additional insertion site. Peste des petits ruminants (PPR) was chosen as a disease model. A new recombinant CaPV expressing the viral attachment hemagglutinin (H) of the PPR virus (PPRV) in the GPCR insertion site (rKS1-HPPR-GPCR) was generated in the backbone North African isolate KS1 strain of lumpy skin disease virus (LSDV). Comparison with the recombinant CaPV expressing the H of PPRV in the TK gene (rKS1-HPPR-TK) shown to induce protection against both PPR and LSD in both sheep and goats was assessed. The suitability of the GPCR gene to be a putative additional insertion site in the CaPV genome is evaluated and discussed.
[Mh] Termos MeSH primário: Capripoxvirus/genética
Vetores Genéticos
Doença Nodular Cutânea/prevenção & controle
Vírus da Doença Nodular Cutânea/genética
Mutagênese Insercional
Peste dos Pequenos Ruminantes/prevenção & controle
Vírus da Peste dos Pequenos Ruminantes/genética
Receptores de Quimiocinas/genética
Receptores Acoplados a Proteínas-G/genética
Vacinas Virais/genética
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/sangue
Bovinos
Cercopithecus aethiops
Cabras
Hemaglutininas Virais/administração & dosagem
Hemaglutininas Virais/genética
Hemaglutininas Virais/imunologia
Injeções Subcutâneas
Doença Nodular Cutânea/genética
Doença Nodular Cutânea/imunologia
Doença Nodular Cutânea/virologia
Vírus da Doença Nodular Cutânea/imunologia
Peste dos Pequenos Ruminantes/genética
Peste dos Pequenos Ruminantes/imunologia
Peste dos Pequenos Ruminantes/virologia
Vírus da Peste dos Pequenos Ruminantes/imunologia
Receptores de Quimiocinas/administração & dosagem
Receptores de Quimiocinas/imunologia
Receptores Acoplados a Proteínas-G/administração & dosagem
Receptores Acoplados a Proteínas-G/imunologia
Vacinas Sintéticas/administração & dosagem
Vacinas Sintéticas/genética
Vacinas Sintéticas/imunologia
Células Vero
Vacinas Virais/administração & dosagem
Vacinas Virais/imunologia
Cultura de Vírus
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Hemagglutinins, Viral); 0 (Receptors, Chemokine); 0 (Receptors, G-Protein-Coupled); 0 (Vaccines, Synthetic); 0 (Viral Vaccines)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170604
[St] Status:MEDLINE


  2 / 152 MEDLINE  
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[PMID]:28430087
[Au] Autor:Das A; Deng MY; Babiuk S; McIntosh MT
[Ad] Endereço:Foreign Animal Disease Diagnostic Laboratory, National Veterinary Services Laboratories, Animal and Plant Health Inspection Services, U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY (Das, Deng, McIntosh).
[Ti] Título:Modification of two capripoxvirus quantitative real-time PCR assays to improve diagnostic sensitivity and include beta-actin as an internal positive control.
[So] Source:J Vet Diagn Invest;29(3):351-356, 2017 May.
[Is] ISSN:1943-4936
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Capripoxviruses (CaPVs), consisting of Sheeppox virus (SPV), Goatpox virus (GPV), and Lumpy skin disease virus (LSDV) species, cause economically significant diseases in sheep, goats, and cattle, respectively. Quantitative real-time polymerase chain reaction (qPCR) assays are routinely used for rapid detection of CaPVs in surveillance and outbreak management programs. We further modified and optimized 2 previously published CaPV qPCR assays, referred to as the Balinsky and Bowden assays, by changing commercial PCR reagents used in the tests. The modified assays displayed 100% analytical specificity and showed no apparent changes in analytical sensitivities for detection of CaPVs compared with the original assays. Diagnostic sensitivities, assessed using 50 clinical reference samples from experimentally infected sheep, goats, and cattle, improved from 82% to 92% for the modified Balinsky assay and from 58% to 82% for the modified Bowden assay. The modified qPCR assays were multiplexed for detection of beta-actin as an indicator for potential false-negative results. The multiplex modified qPCR assays exhibited the same diagnostic sensitivities as the singleplex assays suggesting their utility in the detection of CaPVs.
[Mh] Termos MeSH primário: Capripoxvirus/isolamento & purificação
Surtos de Doenças/veterinária
Infecções por Poxviridae/veterinária
[Mh] Termos MeSH secundário: Actinas/análise
Animais
Capripoxvirus/genética
Bovinos
Doenças dos Bovinos/diagnóstico
Doenças dos Bovinos/virologia
Surtos de Doenças/prevenção & controle
Doenças das Cabras/diagnóstico
Doenças das Cabras/virologia
Cabras
Infecções por Poxviridae/diagnóstico
Infecções por Poxviridae/virologia
Reação em Cadeia da Polimerase em Tempo Real/métodos
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Sensibilidade e Especificidade
Ovinos
Doenças dos Ovinos/diagnóstico
Doenças dos Ovinos/virologia
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170422
[St] Status:MEDLINE
[do] DOI:10.1177/1040638717695609


  3 / 152 MEDLINE  
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[PMID]:28028940
[Au] Autor:Ramakrishnan MA; Santhamani R; Pandey AB
[Ad] Endereço:Division of Virology, Indian Veterinary Research Institute, Mukteswar, Nainital, Uttarakhand, India.
[Ti] Título:Capripox outbreak in a mixed flock of sheep and goats in India.
[So] Source:Transbound Emerg Dis;64(1):27-30, 2017 Feb.
[Is] ISSN:1865-1682
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Generally, capripoxvirus infections are host specific in nature and occasionally infect more than one species. In this study, an investigation was carried out from an outbreak of capripox in a mixed flock of sheep and goats which occurred in 2013 in the State of Jammu & Kashmir. The genetic analysis of P32, RPO30 and GPCR genes revealed that both goats and sheep were infected with goatpox virus.
[Mh] Termos MeSH primário: Capripoxvirus/isolamento & purificação
Surtos de Doenças/veterinária
Doenças das Cabras/epidemiologia
Infecções por Poxviridae/veterinária
Doenças dos Ovinos/epidemiologia
[Mh] Termos MeSH secundário: Animais
Capripoxvirus/genética
Doenças das Cabras/virologia
Cabras
Índia/epidemiologia
Infecções por Poxviridae/epidemiologia
Ovinos
Doenças dos Ovinos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170209
[Lr] Data última revisão:
170209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161229
[St] Status:MEDLINE
[do] DOI:10.1111/tbed.12604


  4 / 152 MEDLINE  
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[PMID]:26608662
[Au] Autor:Armson B; Fowler VL; Tuppurainen ESM; Howson ELA; Madi M; Sallu R; Kasanga CJ; Pearson C; Wood J; Martin P; Mioulet V; King DP
[Ad] Endereço:The Pirbright Institute, Surrey, UK.
[Ti] Título:Detection of Capripoxvirus DNA Using a Field-Ready Nucleic Acid Extraction and Real-Time PCR Platform.
[So] Source:Transbound Emerg Dis;64(3):994-997, 2017 Jun.
[Is] ISSN:1865-1682
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Capripoxviruses, comprising sheep pox virus, goat pox virus and lumpy skin disease virus cause serious diseases of domesticated ruminants, notifiable to The World Organization for Animal Health. This report describes the evaluation of a mobile diagnostic system (Enigma Field Laboratory) that performs automated sequential steps for nucleic acid extraction and real-time PCR to detect capripoxvirus DNA within laboratory and endemic field settings. To prepare stable reagents that could be deployed into field settings, lyophilized reagents were used that employed an established diagnostic PCR assay. These stabilized reagents demonstrated an analytical sensitivity that was equivalent, or greater than the established laboratory-based PCR test which utilizes wet reagents, and the limit of detection for the complete assay pipeline was approximately one log more sensitive than the laboratory-based PCR assay. Concordant results were generated when the mobile PCR system was compared to the laboratory-based PCR using samples collected from Africa, Asia and Europe (n = 10) and experimental studies (n = 9) representing clinical cases of sheep pox, goat pox and lumpy skin disease. Furthermore, this mobile assay reported positive results in situ using specimens that were collected from a dairy cow in Morogoro, Tanzania, which was exhibiting clinical signs of lumpy skin disease. These data support the use of mobile PCR systems for the rapid and sensitive detection of capripoxvirus DNA in endemic field settings.
[Mh] Termos MeSH primário: Capripoxvirus/isolamento & purificação
DNA Viral/isolamento & purificação
Infecções por Poxviridae/veterinária
Reação em Cadeia da Polimerase em Tempo Real/veterinária
[Mh] Termos MeSH secundário: Animais
Capripoxvirus/genética
Bovinos
Doenças dos Bovinos/diagnóstico
Doenças dos Bovinos/virologia
DNA Viral/genética
Feminino
Doenças das Cabras/diagnóstico
Doenças das Cabras/virologia
Cabras
Infecções por Poxviridae/virologia
Reação em Cadeia da Polimerase em Tempo Real/métodos
Sensibilidade e Especificidade
Ovinos
Doenças dos Ovinos/diagnóstico
Doenças dos Ovinos/virologia
Tanzânia/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151127
[St] Status:MEDLINE
[do] DOI:10.1111/tbed.12447


  5 / 152 MEDLINE  
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[PMID]:26564428
[Au] Autor:Tuppurainen ESM; Venter EH; Shisler JL; Gari G; Mekonnen GA; Juleff N; Lyons NA; De Clercq K; Upton C; Bowden TR; Babiuk S; Babiuk LA
[Ad] Endereço:Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.
[Ti] Título:Review: Capripoxvirus Diseases: Current Status and Opportunities for Control.
[So] Source:Transbound Emerg Dis;64(3):729-745, 2017 Jun.
[Is] ISSN:1865-1682
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Lumpy skin disease, sheeppox and goatpox are high-impact diseases of domestic ruminants with a devastating effect on cattle, sheep and goat farming industries in endemic regions. In this article, we review the current geographical distribution, economic impact of an outbreak, epidemiology, transmission and immunity of capripoxvirus. The special focus of the article is to scrutinize the use of currently available vaccines to investigate the resource needs and challenges that will have to be overcome to improve disease control and eradication, and progress on the development of safer and more effective vaccines. In addition, field evaluation of the efficacy of the vaccines and the genomic database available for poxviruses are discussed.
[Mh] Termos MeSH primário: Capripoxvirus
Surtos de Doenças/veterinária
Infecções por Poxviridae/veterinária
[Mh] Termos MeSH secundário: Animais
Capripoxvirus/imunologia
Surtos de Doenças/prevenção & controle
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151114
[St] Status:MEDLINE
[do] DOI:10.1111/tbed.12444


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[PMID]:27529993
[Au] Autor:Selim A; Elhaig M; Höche J; Gaede W
[Ti] Título:Molecular detection and analysis of Sheeppox and Orf viruses isolated from sheep from Qalubia, Egypt.
[So] Source:Berl Munch Tierarztl Wochenschr;129(7-8):310-7, 2016 Jul-Aug.
[Is] ISSN:0005-9366
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In this study an outbreak with Sheeppox virus (SPPV) and Orf virus (ORFV) in one sheep herd in the Qalubia province, Egypt, was investigated. Both, SPPV and ORFV caused clinically manifest infections among sheep. The affected sheep showed skin lesions around the mouth or all over the body. Therefore, reliable diagnosis should confirm the aetiology of the infection and then reduce spread of the diseases in the affected areas. Clinical samples were investigated by virus isolation, PCR and real-time PCR assays. Furthermore, PCR-products of SPPV and ORFV isolates were sequenced and alignment to reference isolates was performed for phylogenetic analyses. The laboratory diagnosis showed that real-time PCR assay was more accurate and sensitive than conventional PCR and virus isolation. In phylogenetic analysis of the A29L gene genetic differences between SPPV field strains were not observed and the strains showed 100% homology with two SPPV isolates from Kazakhstan and one isolate from Turkey. The ORFV field strains are in the P55 gene genetically distinct from another and from other published isolates from Egypt 2006 and 2009.
[Mh] Termos MeSH primário: Capripoxvirus/isolamento & purificação
Ectima Contagioso/virologia
Vírus do Orf/isolamento & purificação
Infecções por Poxviridae/veterinária
Doenças dos Ovinos/virologia
[Mh] Termos MeSH secundário: Animais
Capripoxvirus/classificação
Capripoxvirus/genética
DNA Viral/química
DNA Viral/isolamento & purificação
Ectima Contagioso/epidemiologia
Egito/epidemiologia
Vírus do Orf/classificação
Vírus do Orf/genética
Filogenia
Reação em Cadeia da Polimerase/veterinária
Infecções por Poxviridae/epidemiologia
Infecções por Poxviridae/virologia
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Alinhamento de Sequência/veterinária
Ovinos
Doenças dos Ovinos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160817
[Lr] Data última revisão:
160817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160818
[St] Status:MEDLINE


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[PMID]:27504887
[Au] Autor:Aldaiarov N; Stahel A; Nufer L; Jumakanova Z; Tulobaev A; Ruetten M
[Ad] Endereço:Biology Department of Natural Sciences Faculty, Kyrgyz-Turkish Manas University, Bishkek, Kyrgystan.
[Ti] Título:Outbreak of sheeppox in farmed sheep in Kyrgystan: Histological, eletron microscopical and molecular characterization.
[Ti] Título:Ausbruch von Schafpocken in Kirgistan: eine detaillierte histologische, elektronenmikroskopische und molekulare Charakterisierung..
[So] Source:Schweiz Arch Tierheilkd;158(7):529-32, 2016 Jul.
[Is] ISSN:0036-7281
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: On a farm in the Kyrgyz Republic, several dead sheep were found without any history of illness. The sheep showed several ulcerations on lips and bare-skinned areas. At necropsy the lungs showed multiple firm nodules, which were defined as pox nodules histologically. In the rumen hyperkeratotic plaques were visible. With electron microscopy pox viral particles were detected and confirmed with q PCR as Capripoxviruses. Although all members of the Capripoxvirus genus are eradicated in western countries, this study should remind us of the classical lesions observed in poxvirus infections.
[Mh] Termos MeSH primário: Capripoxvirus/genética
Capripoxvirus/ultraestrutura
Infecções por Poxviridae/veterinária
Doenças dos Ovinos/patologia
Doenças dos Ovinos/virologia
[Mh] Termos MeSH secundário: Animais
Capripoxvirus/isolamento & purificação
Quirguistão
Lábio/patologia
Pulmão/patologia
Microscopia Eletrônica de Transmissão
Infecções por Poxviridae/patologia
Infecções por Poxviridae/virologia
Rúmen/patologia
Ovinos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160810
[St] Status:MEDLINE
[do] DOI:10.17236/sat00076


  8 / 152 MEDLINE  
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[PMID]:27357388
[Au] Autor:Boumart Z; Daouam S; Belkourati I; Rafi L; Tuppurainen E; Tadlaoui KO; El Harrak M
[Ad] Endereço:Research and Development Virology, Multi-Chemical Industry, Lot. 157, Z I, Sud-Ouest (ERAC) B.P.: 278, Mohammedia, 28810, Morocco. Z.Boumart@mci-santeanimale.com.
[Ti] Título:Comparative innocuity and efficacy of live and inactivated sheeppox vaccines.
[So] Source:BMC Vet Res;12(1):133, 2016 Jun 29.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Sheeppox (SPP) is one of the priorities, high-impact animal diseases in many developing countries, where live attenuated vaccines are routinely used against sheeppox virus (SPPV). In an event of an SPP outbreak, historically disease-free countries would hesitate to use of live vaccines against SPPVdue to the safety and trade reasons. Currently no killed SPPV vaccines are commercially available. In this study, we developed an inactivated Romanian SPPVvaccine and assessed its efficacy and potency in comparison with a live attenuated Romanian SPPV vaccine. Four naïve sheep were vaccinated once with the Romanian SPPV live attenuated vaccine and16 sheep were vaccinated twice with the inactivated vaccine. All sheep in the live vaccine group were included in the challenge trial, which was conducted using a highly virulent Moroccan SPPV field strain. Eight sheep of the inactivated vaccine group were challenged and the remaining sheep were monitored for seroconversion. Experimental animals were closely monitored for the appearance of clinical signs, body temperature and inflammation at the injection site. Two naïve sheep were used as unvaccinated controls. RESULTS: The inactivated Romanian SPPV vaccine was found to be safe and confer a good protection, similar to the live vaccine. Specific antibodies appeared from seven days post vaccination and remained up to nine months. CONCLUSION: This study showed that the developed inactivated Romanian SPPV vaccine has a potential to replace attenuated vaccine to control and prevent sheep pox in disease-free or endemic countries.
[Mh] Termos MeSH primário: Capripoxvirus/imunologia
Infecções por Poxviridae/veterinária
Doenças dos Ovinos/prevenção & controle
Vacinas Virais/imunologia
[Mh] Termos MeSH secundário: Animais
Cercopithecus aethiops
Infecções por Poxviridae/imunologia
Infecções por Poxviridae/prevenção & controle
Ovinos
Doenças dos Ovinos/imunologia
Potência de Vacina
Vacinas Atenuadas/imunologia
Vacinas de Produtos Inativados/imunologia
Células Vero
[Pt] Tipo de publicação:CLINICAL TRIAL; COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Vaccines, Attenuated); 0 (Vaccines, Inactivated); 0 (Viral Vaccines)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160701
[St] Status:MEDLINE
[do] DOI:10.1186/s12917-016-0754-0


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[PMID]:27338444
[Au] Autor:Chervyakova OV; Zaitsev VL; Iskakov BK; Tailakova ET; Strochkov VM; Sultankulova KT; Sandybayev NT; Stanbekova GE; Beisenov DK; Abduraimov YO; Mambetaliyev M; Sansyzbay AR; Kovalskaya NY; Nemchinov LG; Hammond RW
[Ad] Endereço:Research Institute for Biological Safety Problems, RK ME&S - Science Committee, Gvardeiskiy 080409, Kazakhstan. ovch@mail.ru.
[Ti] Título:Recombinant Sheep Pox Virus Proteins Elicit Neutralizing Antibodies.
[So] Source:Viruses;8(6), 2016 Jun 07.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The aim of this work was to evaluate the immunogenicity and neutralizing activity of sheep pox virus (SPPV; genus Capripoxvirus, family Poxviridae) structural proteins as candidate subunit vaccines to control sheep pox disease. SPPV structural proteins were identified by sequence homology with proteins of vaccinia virus (VACV) strain Copenhagen. Four SPPV proteins (SPPV-ORF 060, SPPV-ORF 095, SPPV-ORF 117, and SPPV-ORF 122), orthologs of immunodominant L1, A4, A27, and A33 VACV proteins, respectively, were produced in Escherichia coli. Western blot analysis revealed the antigenic and immunogenic properties of SPPV-060, SPPV-095, SPPV-117 and SPPV-122 proteins when injected with adjuvant into experimental rabbits. Virus-neutralizing activity against SPPV in lamb kidney cell culture was detected for polyclonal antisera raised to SPPV-060, SPPV-117, and SPPV-122 proteins. To our knowledge, this is the first report demonstrating the virus-neutralizing activities of antisera raised to SPPV-060, SPPV-117, and SPPV-122 proteins.
[Mh] Termos MeSH primário: Anticorpos Neutralizantes/sangue
Anticorpos Antivirais/sangue
Capripoxvirus/imunologia
Proteínas Recombinantes/imunologia
Proteínas Estruturais Virais/imunologia
Vacinas Virais/imunologia
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos/administração & dosagem
Animais
Western Blotting
Capripoxvirus/genética
Linhagem Celular
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Testes de Neutralização
Coelhos
Proteínas Recombinantes/genética
Ovinos
Vacinas de Subunidades/administração & dosagem
Vacinas de Subunidades/imunologia
Ensaio de Placa Viral
Proteínas Estruturais Virais/genética
Vacinas Virais/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Antibodies, Neutralizing); 0 (Antibodies, Viral); 0 (Recombinant Proteins); 0 (Vaccines, Subunit); 0 (Viral Structural Proteins); 0 (Viral Vaccines)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160625
[St] Status:MEDLINE


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[PMID]:27294125
[Au] Autor:Sevik M; Avci O; Dogan M; Ince ÖB
[Ad] Endereço:Department of Molecular Microbiology, Veterinary Control Institute, Meram, 42080 Konya, Turkey.
[Ti] Título:Serum Biochemistry of Lumpy Skin Disease Virus-Infected Cattle.
[So] Source:Biomed Res Int;2016:6257984, 2016.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lumpy skin disease is an economically important poxvirus disease of cattle. Vaccination is the main method of control but sporadic outbreaks have been reported in Turkey. This study was carried out to determine the changes in serum biochemical values of cattle naturally infected with lumpy skin disease virus (LSDV). For this study, blood samples in EDTA, serum samples, and nodular skin lesions were obtained from clinically infected animals (n = 15) whereas blood samples in EDTA and serum samples were collected from healthy animals (n = 15). A quantitative real-time PCR method was used to detect Capripoxvirus (CaPV) DNA in clinical samples. A real-time PCR high-resolution melt assay was performed to genotype CaPVs. Serum cardiac, hepatic, and renal damage markers and lipid metabolism products were measured by autoanalyzer. LSDV nucleic acid was detected in all samples which were obtained from clinically infected cattle. The results of serum biochemical analysis showed that aspartate aminotransferase, alkaline phosphatase, total protein, and creatinine concentrations were markedly increased in serum from infected animals. However, there were no significant differences in the other biochemical parameters evaluated. The results of the current study suggest that liver and kidney failures occur during LSDV infection. These findings may help in developing effective treatment strategies in LSDV infection.
[Mh] Termos MeSH primário: Doença Nodular Cutânea/sangue
Doença Nodular Cutânea/virologia
Vírus da Doença Nodular Cutânea/patogenicidade
[Mh] Termos MeSH secundário: Fosfatase Alcalina/sangue
Animais
Aspartato Aminotransferases/sangue
Capripoxvirus/genética
Capripoxvirus/patogenicidade
Bovinos
Creatinina/sangue
DNA Viral
Falência Hepática/sangue
Falência Hepática/metabolismo
Falência Hepática/virologia
Doença Nodular Cutânea/metabolismo
Vírus da Doença Nodular Cutânea/genética
Proteínas/metabolismo
Insuficiência Renal/sangue
Insuficiência Renal/metabolismo
Insuficiência Renal/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Proteins); AYI8EX34EU (Creatinine); EC 2.6.1.1 (Aspartate Aminotransferases); EC 3.1.3.1 (Alkaline Phosphatase)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160614
[St] Status:MEDLINE
[do] DOI:10.1155/2016/6257984



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