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Weiblen, Rudi
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[PMID]:27793645
[Au] Autor:Cargnelutti JF; Weiblen R; Flores EF
[Ad] Endereço:Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Av. Roraima, 1000, building 63A, Santa Maria, Rio Grande do Sul, zip-code 97105-900, Brazil.
[Ti] Título:A multiplex PCR for viruses associated with exanthematic and vesicular disease in cattle.
[So] Source:J Virol Methods;239:38-41, 2017 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Exanthematic and papulo-vesicular lesions in the udder and teats of milking cows are fairly common in some Brazilian dairies, especially those with poor sanitary conditions and hand milking. The orthopoxvirus Vaccinia virus (VACV) and the parapoxviruses Pseudocowpox virus (PCPV) and Bovine popular stomatitis virus (BPSV) have been frequently associated with such conditions. Elsewhere, Bovine herpesvirus 2 (BoHV-2) has also been associated with similar clinical signs. Thus, we herein describe a conventional multiplex PCR designed to detect the genome of these viruses in clinical samples while differentiating among them by amplicon size. For this, primer sets targeting the orthopoxvirus vascular growth factor (amplicon size 292bp), PCPV (374bp) and BSPV (607bp) B2L genes, and the BoHV-2 DNA polymerase gene (138bp) were selected. The chosen primers anneal within the same temperature range and do not interfere with each other during the PCR amplification. PCR conditions were initially standardized for each agent in individual PCR reactions firstly using the target virus as positive control followed by using a mixture of all four virues. Lastly, a multiplex PCR containing the four sets of primers was set up to amplify all four targeted viruses in one reaction. The multiplex PCR was able to detect DNA extracted from cell culture supernatants containing 20 TCID of BoHV-2 and 50 TCID of VACV. Further, the test could detect the viral genomes in 1:10, 1:50 and 1:1000 dilutions of total DNA extracted from clinical specimens (e.g. scabs, crusts) of natural cases (PCPV, VACV and BPSV) and 1:10 dilutions of DNA extracted from scabs collected from BoHV-2 experimentally infected cattle. A possible amplification of other orthopoxviruses, predicted by in silico analysis, was considered to not represent an important pitfall since these are exotic in Brazil, very rare, or viruses not associated with cattle. For definitive agent identification amplicon sequencing needs to be conducted. Thus, this multiplex PCR seems suitable for initial detection and identification of the agents involved in exanthematic and vesicular disease, providing a sensitive and specific diagnosis for such conditions in dairy cows.
[Mh] Termos MeSH primário: Doenças dos Bovinos/diagnóstico
Doenças dos Bovinos/virologia
Reação em Cadeia da Polimerase Multiplex/métodos
Infecções por Poxviridae/veterinária
[Mh] Termos MeSH secundário: Animais
Brasil
Bovinos
Primers do DNA
DNA Viral/genética
Genes Virais
Genoma Viral
Orthopoxvirus/genética
Orthopoxvirus/isolamento & purificação
Parapoxvirus/genética
Parapoxvirus/isolamento & purificação
Infecções por Poxviridae/diagnóstico
Infecções por Poxviridae/virologia
Vírus da Pseudovaríola das Vacas/genética
Vírus da Pseudovaríola das Vacas/isolamento & purificação
Vírus Vaccinia/genética
Vírus Vaccinia/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161030
[St] Status:MEDLINE


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[PMID]:27936239
[Au] Autor:Sharif S; Nakatani Y; Wise L; Corbett M; Real NC; Stuart GS; Lateef Z; Krause K; Mercer AA; Fleming SB
[Ad] Endereço:Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
[Ti] Título:A Broad-Spectrum Chemokine-Binding Protein of Bovine Papular Stomatitis Virus Inhibits Neutrophil and Monocyte Infiltration in Inflammatory and Wound Models of Mouse Skin.
[So] Source:PLoS One;11(12):e0168007, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bovine papular stomatitis virus (BPSV) is a Parapoxvirus that induces acute pustular skin lesions in cattle and is transmissible to humans. Previous studies have shown that BPSV encodes a distinctive chemokine-binding protein (CBP). Chemokines are critically involved in the trafficking of immune cells to sites of inflammation and infected tissue, suggesting that the CBP plays a role in immune evasion by preventing immune cells reaching sites of infection. We hypothesised that the BPSV-CBP binds a wide range of inflammatory chemokines particularly those involved in BPSV skin infection, and inhibits the recruitment of immune cells from the blood into inflamed skin. Molecular analysis of the purified protein revealed that the BPSV-CBP is a homodimeric polypeptide with a MW of 82.4 kDa whilst a comprehensive screen of inflammatory chemokines by surface plasmon resonance showed high-affinity binding to a range of chemokines within the CXC, CC and XC subfamilies. Structural analysis of BPSV-CBP, based on the crystal structure of orf virus CBP, provided a probable explanation for these chemokine specificities at a molecular level. Functional analysis of the BPSV-CBP using transwell migration assays demonstrated that it potently inhibited chemotaxis of murine neutrophils and monocytes in response to CXCL1, CXCL2 as well as CCL2, CCL3 and CCL5 chemokines. In order to examine the effects of CBP in vivo, we used murine skin models to determine its impact on inflammatory cell recruitment such as that observed during BPSV infection. Intradermal injection of BPSV-CBP blocked the influx of neutrophils and monocytes in murine skin in which inflammation was induced with lipopolysaccharide. Furthermore, intradermal injection of BPSV-CBP into injured skin, which more closely mimics BPSV lesions, delayed the influx of neutrophils and reduced the recruitment of MHC-II+ immune cells to the wound bed. Our findings suggest that the CBP could be important in pathogenesis of BPSV infections.
[Mh] Termos MeSH primário: Quimiocinas/metabolismo
Quimiotaxia de Leucócito/fisiologia
Modelos Animais de Doenças
Inflamação/patologia
Monócitos/patologia
Neutrófilos/patologia
Parapoxvirus/metabolismo
Proteínas Virais/fisiologia
Ferimentos e Lesões/patologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Dimerização
Camundongos
Conformação Proteica
Homologia de Sequência de Aminoácidos
Ressonância de Plasmônio de Superfície
Proteínas Virais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chemokines); 0 (Viral Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161210
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0168007


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[PMID]:27751948
[Au] Autor:Kurosaki Y; Okada S; Nakamae S; Yasuda J
[Ad] Endereço:Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki 852-8523, Japan. Electronic address: ykuro@nagasaki-u.ac.jp.
[Ti] Título:A loop-mediated isothermal amplification assay for rapid and sensitive detection of bovine papular stomatitis virus.
[So] Source:J Virol Methods;238:42-47, 2016 Dec.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Bovine papular stomatitis virus (BPSV) causes pustular cutaneous disease in cattle worldwide. This paper describes the development of a specific loop-mediated isothermal amplification (LAMP) assay to detect BPSV which did not cross-react with other parapoxviruses. To assess analytical sensitivity of this LAMP assay, DNA was extracted from serially diluted BPSV from which the infectious titer was determined by a novel assay based on calf kidney epithelial cells. The LAMP assay had equivalent analytical sensitivity to quantitative PCR, and could detect as few as 86 copies of viral DNA per reaction. These results suggest that the assay is a specific and sensitive technique to rapidly diagnose bovine papular stomatitis in domestic animals.
[Mh] Termos MeSH primário: Doenças dos Bovinos/diagnóstico
Técnicas de Amplificação de Ácido Nucleico/métodos
Parapoxvirus/genética
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos/virologia
Primers do DNA/genética
DNA Viral/análise
Células Epiteliais/virologia
Limite de Detecção
Parapoxvirus/isolamento & purificação
Infecções por Poxviridae/diagnóstico
Infecções por Poxviridae/virologia
RNA Viral
Reação em Cadeia da Polimerase em Tempo Real
Sensibilidade e Especificidade
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Viral); 0 (RNA, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE


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[PMID]:27558814
[Au] Autor:Hain KS; Joshi LR; Okda F; Nelson J; Singrey A; Lawson S; Martins M; Pillatzki A; Kutish GF; Nelson EA; Flores EF; Diel DG
[Ad] Endereço:1​Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.
[Ti] Título:Immunogenicity of a recombinant parapoxvirus expressing the spike protein of Porcine epidemic diarrhea virus.
[So] Source:J Gen Virol;97(10):2719-2731, 2016 Oct.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The parapoxvirus Orf virus (ORFV), has long been recognized for its immunomodulatory properties in permissive and non-permissive animal species. Here, a new recombinant ORFV expressing the full-length spike (S) protein of Porcine epidemic diarrhea virus (PEDV) was generated and its immunogenicity and protective efficacy were evaluated in pigs. The PEDV S was inserted into the ORFV121 gene locus, an immunomodulatory gene that inhibits activation of the NF-κB signalling pathway and contributes to ORFV virulence in the natural host. The recombinant ORFV-PEDV-S virus efficiently and stably expressed the PEDV S protein in cell culture in vitro. Three intramuscular (IM) immunizations with the recombinant ORFV-PEDV-S in 3-week-old pigs elicited robust serum IgG, IgA and neutralizing antibody responses against PEDV. Additionally, IM immunization with the recombinant ORFV-PEDV-S virus protected pigs from clinical signs of porcine epidemic diarrhoea (PED) and reduced virus shedding in faeces upon challenge infection. These results demonstrate the suitability of ORFV121 gene locus as an insertion site for heterologous gene expression and delivery by ORFV-based viral vectors. Additionally, the results provide evidence of the potential of ORFV as a vaccine delivery vector for enteric viral diseases of swine. This study may have important implications for future development of ORFV-vectored vaccines for swine.
[Mh] Termos MeSH primário: Infecções por Coronavirus/veterinária
Vírus da Diarreia Epidêmica Suína/imunologia
Glicoproteína da Espícula de Coronavírus/imunologia
Doenças dos Suínos/imunologia
Vacinas Virais/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Neutralizantes/imunologia
Anticorpos Antivirais/imunologia
Infecções por Coronavirus/imunologia
Infecções por Coronavirus/prevenção & controle
Infecções por Coronavirus/virologia
Vetores Genéticos/genética
Vetores Genéticos/metabolismo
Imunização
Parapoxvirus/genética
Parapoxvirus/metabolismo
Vírus da Diarreia Epidêmica Suína/genética
Glicoproteína da Espícula de Coronavírus/administração & dosagem
Glicoproteína da Espícula de Coronavírus/genética
Suínos
Doenças dos Suínos/prevenção & controle
Doenças dos Suínos/virologia
Vacinas Virais/administração & dosagem
Vacinas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Neutralizing); 0 (Antibodies, Viral); 0 (Spike Glycoprotein, Coronavirus); 0 (Viral Vaccines)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160826
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000586


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[PMID]:27154321
[Au] Autor:Laguardia-Nascimento M; Sales ÉB; Gasparini MR; de Souza NM; da Silva JA; Souza GG; Carani FR; Dos Santos AF; Rivetti Júnior AV; Camargos MF; Fonseca Júnior AA
[Ad] Endereço:Laboratório Nacional Agropecuário de Minas Gerais, Pedro Leopoldo, Minas Gerais, Brazil (Laguardia-Nascimento, Sales, Gasparini, de Souza, Rivetti Júnior, Camargos, Fonseca Júnior)Instituto de Defesa Agropecuária do Estado de Mato Grosso, Cuiabá, Mato Grosso, Brazil (da Silva, Souza, Carani)Agência
[Ti] Título:Detection of multiple viral infections in cattle and buffalo with suspected vesicular disease in Brazil.
[So] Source:J Vet Diagn Invest;28(4):377-81, 2016 Jul.
[Is] ISSN:1943-4936
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Vesicular diseases are of high importance for livestock, primarily because of foot-and-mouth disease (FMD), which is a high-morbidity disease that generates direct losses caused by low milk production, weight loss, and indirect losses because of the need for sanitary barriers. Other vesicular diseases are also of importance for livestock because of direct impacts or because their clinical signs may be confused with those of FMD. We report herein the detection of multiple infections in cattle with suspected vesicular disease in the Brazilian states of Amazonas (AM), Mato Grosso (MT), and Roraima. Thirty-seven epithelial samples from cattle and 1 sample from a buffalo were sent to the laboratory for testing for FMDV and similar disease agents. All samples from MT were positive for parapoxvirus (Pseudocowpox virus and Bovine papular stomatitis virus). In addition, 3 samples were positive for Bluetongue virus, and 5 samples were positive for Bovine herpesvirus 1 Among these samples, 1 was positive for all of these 3 agents. Only 2 samples from AM were negative for parapoxvirus. The molecular tests conducted in this study detected multiple infections, with a high prevalence of parapoxvirus.
[Mh] Termos MeSH primário: Bluetongue/diagnóstico
Búfalos
Doenças dos Bovinos/diagnóstico
Infecções por Herpesviridae/veterinária
Infecções por Poxviridae/veterinária
[Mh] Termos MeSH secundário: Animais
Bluetongue/virologia
Vírus Bluetongue/isolamento & purificação
Brasil/epidemiologia
Bovinos
Doenças dos Bovinos/virologia
Infecções por Herpesviridae/diagnóstico
Infecções por Herpesviridae/virologia
Herpesvirus Bovino 1/isolamento & purificação
Parapoxvirus/isolamento & purificação
Infecções por Poxviridae/diagnóstico
Infecções por Poxviridae/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160508
[St] Status:MEDLINE
[do] DOI:10.1177/1040638716645836


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[PMID]:27027837
[Au] Autor:Yaegashi G; Fukunari K; Oyama T; Murakami RK; Inoshima Y
[Ti] Título:Detection and quantification of parapoxvirus DNA by use of a quantitative real-time polymerase chain reaction assay in calves without clinical signs of parapoxvirus infection.
[So] Source:Am J Vet Res;77(4):383-7, 2016 Apr.
[Is] ISSN:1943-5681
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To investigate the presence of parapoxvirus (PPV) in cattle without clinical signs of infection and in farm environments of PPV-infected cattle. ANIMALS: 28 calves without clinical signs of PPV infection on 2 farms and 11 clinically affected calves on 6 farms. PROCEDURES: 164 oral swab samples were collected at regular intervals from 28 calves without clinical signs of PPV infection, and 11 swab samples were collected from 11 clinically affected calves. Viral DNA load was quantified by use of a PPV-specific quantitative real-time PCR (qRT-PCR) assay. RESULTS Of 28 calves without clinical signs of PPV infection, 12 had positive results for PPV DNA by use of the qRT-PCR assay. Viral DNA was detected continuously over a period of 2 to 5 months from 9 of these 12 calves, particularly from calves with dermatomycosis or respiratory tract disease. The PPV DNA loads in 32 oral swab samples from these 12 calves were significantly lower (median, 3.2 copies/mg) than those in samples collected from the 11 clinically affected calves (median, 3.2 × 10(4) copies/mg). Moreover, PPV DNA was detected in the residual feed and drinking water on both farms that housed the calves without clinical signs of PPV infection. CONCLUSIONS AND CLINICAL RELEVANCE: PPV in cattle without clinical signs of infection and in the environments of these cattle may represent sources of PPV transmission to susceptible cattle. IMPACT FOR HUMAN MEDICINE: Humans should wear gloves to prevent zoonotic disease transmission when handling cattle with or without clinical signs of PPV infection.
[Mh] Termos MeSH primário: Doenças dos Bovinos/virologia
DNA Viral/análise
Parapoxvirus/isolamento & purificação
Infecções por Poxviridae/veterinária
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Bovinos
Parapoxvirus/genética
Infecções por Poxviridae/virologia
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Carga Viral
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160331
[St] Status:MEDLINE
[do] DOI:10.2460/ajvr.77.4.383


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[PMID]:26458837
[Au] Autor:Rziha HJ; Rohde J; Amann R
[Ad] Endereço:Institute of Immunology, Friedrich-Loeffler-Institute, Südufer 10, Island of Riems, Greifswald, Germany. achim.rziha@fli.bund.de.
[Ti] Título:Generation and Selection of Orf Virus (ORFV) Recombinants.
[So] Source:Methods Mol Biol;1349:177-200, 2016.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Orf virus (ORFV) is an epitheliotropic poxvirus, which belongs to the genus Parapoxvirus. Among them the highly attenuated, apathogenic strain D1701-V is regarded as a promising candidate for novel virus vector vaccines. Our recent work demonstrated that those ORFV-based recombinants were able to induce protective, long-lasting immunity in various hosts that are non-permissive for ORFV. In this chapter we describe procedures for the generation, selection, propagation, and titration of ORFV recombinants as well as transgene detection by PCR or immunohistochemical staining.
[Mh] Termos MeSH primário: Anticorpos Antivirais/imunologia
Vírus do Orf/genética
Vacinas Virais/genética
[Mh] Termos MeSH secundário: Anticorpos Antivirais/genética
Vetores Genéticos
Seres Humanos
Vírus do Orf/imunologia
Parapoxvirus/genética
Parapoxvirus/imunologia
Vacinas Sintéticas/genética
Vacinas Sintéticas/imunologia
Vacinas Sintéticas/uso terapêutico
Proteínas Virais/genética
Proteínas Virais/imunologia
Vacinas Virais/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Vaccines, Synthetic); 0 (Viral Proteins); 0 (Viral Vaccines)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:151013
[Lr] Data última revisão:
151013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151014
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-3008-1_12


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[PMID]:26290129
[Au] Autor:Avci O; Bulut O; Dik I
[Ad] Endereço:Department of Virology, Faculty of Veterinary Medicine, University of Selcuk, 42003 Konya, Turkey.
[Ti] Título:Effects of inactive parapoxvirus ovis on cytokine levels in rats.
[So] Source:J Vet Med Sci;78(1):129-31, 2016 Jan.
[Is] ISSN:1347-7439
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The aim of this study is to determine the effects of iPPOV on pro-inflammatory and anti-inflammatory cytokine levels in rats. iPPOV (1 ml/rat) was administered intraperitoneal route to 49 rats, except for 7 rats (Control, 0 group). Serum samples were collected from 7 rats at 1st, 2nd, 4th, 8th, 12th, 16th and 24th hr after treatments. Levels of TNF-α, IL-6, IL-12 and IL-10 were determined using ELISA. Administration of iPPOV stimulated TNF-α (16th and 24th hr) and IL-6 (12th, 16th and 24th hr) synthesis and caused fluctuations in IL-10 and IL-12 concentrations. In conclusion, increased cytokine levels could be attributed to immunomodulatory activity of iPPOV, however, detailed studies are required to fully understand effects of iPPOV on immune system.
[Mh] Termos MeSH primário: Citocinas/sangue
Parapoxvirus
Infecções por Poxviridae/veterinária
[Mh] Termos MeSH secundário: Animais
Ensaio de Imunoadsorção Enzimática/veterinária
Interleucina-10/sangue
Interleucina-12/sangue
Interleucina-6/sangue
Masculino
Infecções por Poxviridae/sangue
Infecções por Poxviridae/imunologia
Ratos/virologia
Ratos Wistar
Fator de Necrose Tumoral alfa/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokines); 0 (Interleukin-6); 0 (Tumor Necrosis Factor-alpha); 130068-27-8 (Interleukin-10); 187348-17-0 (Interleukin-12)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150821
[St] Status:MEDLINE
[do] DOI:10.1292/jvms.15-0231


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[PMID]:26656974
[Au] Autor:Paulsen D; Weber O; Ruebsamen-Schaeff H; Tennant BC; Menne S
[Ad] Endereço:AiCuris GmbH & Co KG., Wuppertal, Germany.
[Ti] Título:AIC649 Induces a Bi-Phasic Treatment Response in the Woodchuck Model of Chronic Hepatitis B.
[So] Source:PLoS One;10(12):e0144383, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:AIC649 has been shown to directly address the antigen presenting cell arm of the host immune defense leading to a regulated cytokine release and activation of T cell responses. In the present study we analyzed the antiviral efficacy of AIC649 as well as its potential to induce functional cure in animal models for chronic hepatitis B. Hepatitis B virus transgenic mice and chronically woodchuck hepatitis virus (WHV) infected woodchucks were treated with AIC649, respectively. In the mouse system AIC649 decreased the hepatitis B virus titer as effective as the "gold standard", Tenofovir. Interestingly, AIC649-treated chronically WHV infected woodchucks displayed a bi-phasic pattern of response: The marker for functional cure--hepatitis surface antigen--first increased but subsequently decreased even after cessation of treatment to significantly reduced levels. We hypothesize that the observed bi-phasic response pattern to AIC649 treatment reflects a physiologically "concerted", reconstituted immune response against WHV and therefore may indicate a potential for inducing functional cure in HBV-infected patients.
[Mh] Termos MeSH primário: Antivirais/uso terapêutico
Antígenos de Superfície da Hepatite B/metabolismo
Vírus da Hepatite B da Marmota/imunologia
Hepatite B Crônica/imunologia
Marmota/imunologia
[Mh] Termos MeSH secundário: Animais
Terapia Biológica
Biomarcadores/metabolismo
Células Dendríticas/imunologia
Modelos Animais de Doenças
Vírus da Hepatite B/imunologia
Hepatite B Crônica/tratamento farmacológico
Imunidade Celular/imunologia
Interferon-alfa/imunologia
Interferon gama/imunologia
Células Matadoras Naturais/imunologia
Ativação Linfocitária/imunologia
Marmota/virologia
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Transgênicos
Parapoxvirus/imunologia
Linfócitos T/imunologia
Tenofovir/uso terapêutico
Fator de Necrose Tumoral alfa/imunologia
Fator de Necrose Tumoral alfa/secreção
Vacinas de Produtos Inativados/imunologia
Replicação Viral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Biomarkers); 0 (Hepatitis B Surface Antigens); 0 (Interferon-alpha); 0 (Tumor Necrosis Factor-alpha); 0 (Vaccines, Inactivated); 82115-62-6 (Interferon-gamma); 99YXE507IL (Tenofovir)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151215
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0144383


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[PMID]:26260127
[Au] Autor:Khalafalla AI; El-Sabagh IM; Al-Busada KA; Al-Mubarak AI; Ali YH
[Ad] Endereço:Camel Research Center, King Faisal University, Al-Ahsa, 31982, Saudi Arabia. abdokhlf@yahoo.co.uk.
[Ti] Título:Phylogenetic analysis of eight sudanese camel contagious ecthyma viruses based on B2L gene sequence.
[So] Source:Virol J;12:124, 2015 Aug 12.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Camel contagious ecthyma (CCE) is an important viral disease of camelids caused by a poxvirus of the genus parapoxvirus (PPV) of the family Poxviridae. The disease has been reported in west and east of the Sudan causing economical losses. However, the PPVs that cause the disease in camels of the Sudan have not yet subjected to genetic characterization. At present, the PPV that cause CCE cannot be properly classified because only few isolates that have been genetically analyzed. METHODS AND RESULTS: PCR was used to amplify the B2L gene of the PPV directly from clinical specimens collected from dromedary camels affected with contagious ecthyma in the Sudan between 1993 and 2013. PCR products were sequenced and subjected to genetic analysis. The results provided evidence for close relationships and genetic variation of the camel PPV (CPPV) represented by the circulation of both Pseudocowpox virus (PCPV) and Orf virus (ORFV) strains among dromedary camels in the Sudan. Based on the B2L gene sequence the available CPPV isolates can be divided into two genetic clades or lineages; the Asian lineage represented by isolates from Saudi Arabia, Bahrain and India and the African lineage comprising isolates from the Sudan. CONCLUSION: The camel parapoxvirus is genetically diverse involving predominantly viruses close to PCPV in addition to ORFVs, and can be divided into two genetically distant lineages. Based on sequences of the B2L gene it is not possible to suggest that the viruses that cause CCE form a monophylogenetic group or species within the PPV phylogeny.
[Mh] Termos MeSH primário: Ectima Contagioso/virologia
Genes Virais
Parapoxvirus/classificação
Parapoxvirus/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Composição de Bases
Camelus
Análise por Conglomerados
DNA Viral
Fases de Leitura Aberta
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150812
[St] Status:MEDLINE
[do] DOI:10.1186/s12985-015-0348-7



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