Base de dados : MEDLINE
Pesquisa : B04.423 [Categoria DeCS]
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  1 / 1394 MEDLINE  
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[PMID]:29073276
[Au] Autor:Chaturvedi S; Rao ALN
[Ad] Endereço:Department of Microbiology & Plant Pathology, University of California, Riverside, California, United States of America.
[Ti] Título:Riboproteomics: A versatile approach for the identification of host protein interaction network in plant pathogenic noncoding RNAs.
[So] Source:PLoS One;12(10):e0186703, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pathogenic or non-pathogenic small (17 to 30 nt) and long (>200 nt) non-coding RNAs (ncRNAs) have been implicated in the regulation of gene expression at transcriptional, post-transcriptional and epigenetic level by interacting with host proteins. However, lack of suitable experimental system precludes the identification and evaluation of the functional significance of host proteins interacting with ncRNAs. In this study, we present a first report on the application of riboproteomics to identify host proteins interacting with small, highly pathogenic, noncoding satellite RNA (sat-RNA) associated with Cucumber mosaic virus, the helper virus (HV). RNA affinity beads containing sat-RNA transcripts of (+) or (-)-sense covalently coupled to cyanogen bromide activated sepharose beads were incubated with total protein extracts from either healthy or HV-infected Nicotiana benthamiana leaves. RNA-protein complexes bound to the beads were eluted and subjected to MudPIT analysis. Bioinformatics programs PANTHER classification and WoLF-PSORT were used to further classify the identified host proteins in each case based on their functionality and subcellular distribution. Finally, we observed that the host protein network interacting with plus and minus-strand transcripts of sat-RNA, in the presence or absence of HV is distinct, and the global interactome of host proteins interacting with satRNA in either of the orientations is very different.
[Mh] Termos MeSH primário: Cucumovirus/metabolismo
Vírus Auxiliares/metabolismo
Proteínas de Plantas/metabolismo
RNA não Traduzido/metabolismo
RNA Viral/metabolismo
Tabaco/metabolismo
[Mh] Termos MeSH secundário: Cucumovirus/genética
Vírus Auxiliares/genética
Proteínas de Plantas/classificação
Proteínas de Plantas/genética
Proteômica/métodos
RNA não Traduzido/classificação
RNA não Traduzido/genética
RNA Viral/classificação
RNA Viral/genética
Tabaco/genética
Tabaco/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 0 (RNA, Untranslated); 0 (RNA, Viral)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171027
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186703


  2 / 1394 MEDLINE  
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[PMID]:28515305
[Au] Autor:Franzoso FD; Seyffert M; Vogel R; Yakimovich A; de Andrade Pereira B; Meier AF; Sutter SO; Tobler K; Vogt B; Greber UF; Büning H; Ackermann M; Fraefel C
[Ad] Endereço:Institute of Virology, University of Zurich, Zurich, Switzerland.
[Ti] Título:Cell Cycle-Dependent Expression of Adeno-Associated Virus 2 (AAV2) Rep in Coinfections with Herpes Simplex Virus 1 (HSV-1) Gives Rise to a Mosaic of Cells Replicating either AAV2 or HSV-1.
[So] Source:J Virol;91(15), 2017 Aug 01.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Adeno-associated virus 2 (AAV2) depends on the simultaneous presence of a helper virus such as herpes simplex virus 1 (HSV-1) for productive replication. At the same time, AAV2 efficiently blocks the replication of HSV-1, which would eventually limit its own replication by diminishing the helper virus reservoir. This discrepancy begs the question of how AAV2 and HSV-1 can coexist in a cell population. Here we show that in coinfected cultures, AAV2 DNA replication takes place almost exclusively in S/G -phase cells, while HSV-1 DNA replication is restricted to G phase. Live microscopy revealed that not only wild-type AAV2 (wtAAV2) replication but also reporter gene expression from both single-stranded and double-stranded (self-complementary) recombinant AAV2 vectors preferentially occurs in S/G -phase cells, suggesting that the preference for S/G phase is independent of the nature of the viral genome. Interestingly, however, a substantial proportion of S/G -phase cells transduced by the double-stranded but not the single-stranded recombinant AAV2 vectors progressed through mitosis in the absence of the helper virus. We conclude that cell cycle-dependent AAV2 expression facilitates cell cycle-dependent AAV2 DNA replication and inhibits HSV-1 DNA replication. This may limit competition for cellular and viral helper factors and, hence, creates a biological niche for either virus to replicate. Adeno-associated virus 2 (AAV2) differs from most other viruses, as it requires not only a host cell for replication but also a helper virus such as an adenovirus or a herpesvirus. This situation inevitably leads to competition for cellular resources. AAV2 has been shown to efficiently inhibit the replication of helper viruses. Here we present a new facet of the interaction between AAV2 and one of its helper viruses, herpes simplex virus 1 (HSV-1). We observed that AAV2 gene expression is cell cycle dependent and gives rise to distinct time-controlled windows for HSV-1 replication. High Rep protein levels in S/G phase support AAV2 replication and inhibit HSV-1 replication. Conversely, low Rep protein levels in G phase permit HSV-1 replication but are insufficient for AAV2 replication. This allows both viruses to productively replicate in distinct sets of dividing cells.
[Mh] Termos MeSH primário: Ciclo Celular
Proteínas de Ligação a DNA/metabolismo
Dependovirus/crescimento & desenvolvimento
Vírus Auxiliares/crescimento & desenvolvimento
Herpesvirus Humano 1/crescimento & desenvolvimento
Interferência Viral
Proteínas Virais/metabolismo
Replicação Viral
[Mh] Termos MeSH secundário: Linhagem Celular
Coinfecção
Expressão Gênica
Seres Humanos
Microscopia
Cultura de Vírus
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA-Binding Proteins); 0 (Viral Proteins); 137750-19-7 (rep proteins, Adeno-associated virus 2)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170519
[St] Status:MEDLINE


  3 / 1394 MEDLINE  
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[PMID]:28400210
[Au] Autor:Hill RLL; Vlach J; Parker LK; Christie GE; Saad JS; Dokland T
[Ad] Endereço:Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
[Ti] Título:Derepression of SaPIbov1 Is Independent of φNM1 Type 2 dUTPase Activity and Is Inhibited by dUTP and dUMP.
[So] Source:J Mol Biol;429(10):1570-1580, 2017 May 19.
[Is] ISSN:1089-8638
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Staphylococcus aureus is an opportunistic human pathogen able to transfer virulence genes to other cells through the mobilization of S. aureus pathogenicity islands (SaPIs). SaPIs are derepressed and packaged into phage-like transducing particles by helper phages like 80α or φNM1. Phages 80α and φNM1 encode structurally distinct dUTPases, Dut (type 1) and Dut (type 2). Both dUTPases can interact with the SaPIbov1 Stl master repressor, leading to derepression and mobilization. That two structurally distinct dUTPases bind the same repressor led us to speculate that dUTPase activity may be important to the derepression process. In type 1 dUTPases, Stl binding is inhibited by dUTP. The purpose of this study was to assess the involvement of dUTP binding and dUTPase activity in derepression by Dut . Dut activity mutants were created and tested for dUTPase activity using a novel NMR-based assay. We found that all Dut null activity mutants interacted with the SaPIbov1 Stl C-terminal domain, formed Dut -Stl heterodimers, and caused the release of the P promoter. However, promoter release was inhibited in the presence of dUTP or dUMP. We tested two φNM1 mutant phages that had null enzyme activity and found that they could still mobilize SaPIbov1. These results show that only the apo form of Dut is active in Stl derepression and that dUTPase activity is not necessary for the mobilization of SaPIbov1 by Dut .
[Mh] Termos MeSH primário: Nucleotídeos de Desoxiuracil/metabolismo
Ilhas Genômicas
Vírus Auxiliares/enzimologia
Pirofosfatases/metabolismo
Proteínas Repressoras/metabolismo
Staphylococcus aureus/metabolismo
[Mh] Termos MeSH secundário: Bacteriófagos/enzimologia
Inibidores Enzimáticos/metabolismo
Técnicas de Inativação de Genes
Ligação Proteica
Pirofosfatases/genética
Staphylococcus aureus/genética
Staphylococcus aureus/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Deoxyuracil Nucleotides); 0 (Enzyme Inhibitors); 0 (Repressor Proteins); 1173-82-6 (deoxyuridine triphosphate); 964-26-1 (2'-deoxyuridylic acid); EC 3.6.1.- (Pyrophosphatases); EC 3.6.1.23 (dUTP pyrophosphatase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170715
[Lr] Data última revisão:
170715
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170413
[St] Status:MEDLINE


  4 / 1394 MEDLINE  
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[PMID]:28355224
[Au] Autor:Barajas D; Aponte-Ubillus JJ; Akeefe H; Cinek T; Peltier J; Gold D
[Ad] Endereço:BioMarin Pharmaceutical Inc., Novato, California, United States.
[Ti] Título:Generation of infectious recombinant Adeno-associated virus in Saccharomyces cerevisiae.
[So] Source:PLoS One;12(3):e0173010, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The yeast Saccharomyces cerevisiae has been successfully employed to establish model systems for a number of viruses. Such model systems are powerful tools to study the virus biology and in particular for the identification and characterization of host factors playing a role in the viral infection cycle. Adeno-associated viruses (AAV) are heavily studied due to their use as gene delivery vectors. AAV relies on other helper viruses for successful replication and on host factors for several aspects of the viral life cycle. However the role of host and helper viral factors is only partially known. Production of recombinant AAV (rAAV) vectors for gene delivery applications depends on knowledge of AAV biology and the limited understanding of host and helper viral factors may be precluding efficient production, particularly in heterologous systems. Model systems in simpler eukaryotes like the yeast S. cerevisiae would be useful tools to identify and study the role of host factors in AAV biology. Here we show that expression of AAV2 viral proteins VP1, VP2, VP3, AAP, Rep78, Rep52 and an ITR-flanked DNA in yeast leads to capsid formation, DNA replication and encapsidation, resulting in formation of infectious particles. Many of the AAV characteristics observed in yeast resemble those in other systems, making it a suitable model system. Future findings in the yeast system could be translatable to other AAV host systems and aid in more efficient production of rAAV vectors.
[Mh] Termos MeSH primário: DNA Viral/genética
Dependovirus/genética
Regulação Viral da Expressão Gênica
Saccharomyces cerevisiae/virologia
Vírion/genética
[Mh] Termos MeSH secundário: Capsídeo/química
Capsídeo/metabolismo
Proteínas do Capsídeo/genética
Proteínas do Capsídeo/metabolismo
DNA Viral/metabolismo
Proteínas de Ligação a DNA/genética
Proteínas de Ligação a DNA/metabolismo
Dependovirus/crescimento & desenvolvimento
Dependovirus/metabolismo
Vetores Genéticos/química
Vetores Genéticos/metabolismo
Células HEK293
Vírus Auxiliares/genética
Vírus Auxiliares/metabolismo
Interações Hospedeiro-Patógeno
Seres Humanos
Proteínas Virais de Fusão/genética
Proteínas Virais de Fusão/metabolismo
Proteínas Virais/genética
Proteínas Virais/metabolismo
Vírion/crescimento & desenvolvimento
Vírion/metabolismo
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (DNA, Viral); 0 (DNA-Binding Proteins); 0 (VP3 protein, Dependovirus); 0 (Viral Fusion Proteins); 0 (Viral Proteins); 137750-19-7 (rep proteins, Adeno-associated virus 2)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170824
[Lr] Data última revisão:
170824
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173010


  5 / 1394 MEDLINE  
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[PMID]:27702772
[Au] Autor:Chang CH; Hsu FC; Lee SC; Lo YS; Wang JD; Shaw J; Taliansky M; Chang BY; Hsu YH; Lin NS
[Ad] Endereço:Institute of Plant Biology, National Taiwan University, Taipei 11106, Taiwan.
[Ti] Título:The Nucleolar Fibrillarin Protein Is Required for Helper Virus-Independent Long-Distance Trafficking of a Subviral Satellite RNA in Plants.
[So] Source:Plant Cell;28(10):2586-2602, 2016 Oct.
[Is] ISSN:1532-298X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RNA trafficking plays pivotal roles in regulating plant development, gene silencing, and adaptation to environmental stress. Satellite RNAs (satRNAs), parasites of viruses, depend on their helper viruses (HVs) for replication, encapsidation, and efficient spread. However, it remains largely unknown how satRNAs interact with viruses and the cellular machinery to undergo trafficking. Here, we show that the P20 protein of Bamboo mosaic potexvirus satRNA (satBaMV) can functionally complement in trans the systemic trafficking of P20-defective satBaMV in infected Nicotiana benthamiana The transgene-derived satBaMV, uncoupled from HV replication, was able to move autonomously across a graft union identified by RT-qPCR, RNA gel blot, and in situ RT-PCR analyses. Coimmunoprecipitation experiments revealed that the major nucleolar protein fibrillarin is coprecipitated in the P20 protein complex. Notably, silencing fibrillarin suppressed satBaMV-, but not HV-, phloem-based movement following grafting or coinoculation with HV Confocal microscopy revealed that the P20 protein colocalized with fibrillarin in the nucleoli and formed punctate structures associated with plasmodesmata. The mobile satBaMV RNA appears to exist as ribonucleoprotein (RNP) complex composed of P20 and fibrillarin, whereas BaMV movement proteins, capsid protein, and BaMV RNA are recruited with HV coinfection. Taken together, our findings provide insight into movement of satBaMV via the fibrillarin-satBaMV-P20 RNP complex in phloem-mediated systemic trafficking.
[Mh] Termos MeSH primário: Vírus Auxiliares/genética
RNA de Plantas/genética
RNA Satélite/genética
Ribonucleoproteínas/metabolismo
Proteínas Virais/genética
[Mh] Termos MeSH secundário: Imunoprecipitação
Reação em Cadeia da Polimerase Via Transcriptase Reversa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Plant); 0 (RNA, Satellite); 0 (Ribonucleoproteins); 0 (Viral Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161006
[St] Status:MEDLINE


  6 / 1394 MEDLINE  
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[PMID]:27654667
[Au] Autor:Wang Y; Fang L; Li J; Li Y; Cui S; Sun X; Chang S; Zhao P; Cui Z
[Ad] Endereço:College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, Tai'an, Shandong, 271018, China.
[Ti] Título:Rescue of avian leukosis subgroup-J-associated acutely transforming viruses carrying different lengths of the v-fps oncogene and analysis of their tumorigenicity.
[So] Source:Arch Virol;161(12):3473-3481, 2016 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:In our previous study, six subgroup J strains of avian leukosis virus (ALV-J)-associated acutely transforming viruses carrying different lengths of the v-fps oncogene, designated as Fu-J and Fu-J1-5, were isolated and characterized from fibrosarcomas in ALV-J-infected chickens. In the present study, the oncogenic potential of Fu-J and Fu-J1-5 was investigated using a reverse genetics technique. Six replication-defective viruses, named rFu-J and rFu-J1-5, were rescued with the replication-competent rescued ALV-J strain rSDAU1005 as a helper virus by co-transfection of chicken embryo fibroblast monolayers with infectious clone plasmids. Experimental bird studies were performed, demonstrating that only the rescued rFu-J virus carrying the complete v-fps oncogene with rSDAU1005 as the helper virus could induce acute fibrosarcoma after inoculation in specific-pathogen-free (SPF) chickens. These results provide direct evidence that the replication-defective acutely transforming Fu-J virus, with the complete v-fps oncogene, was associated with acute fibrosarcoma in chickens infected with ALV-J in the field, as reported previously.
[Mh] Termos MeSH primário: Vírus da Leucose Aviária/genética
Vírus da Leucose Aviária/isolamento & purificação
Fibrossarcoma/veterinária
Proteínas Oncogênicas/genética
Doenças das Aves Domésticas/patologia
Doenças das Aves Domésticas/virologia
[Mh] Termos MeSH secundário: Experimentação Animal
Animais
Vírus da Leucose Aviária/patogenicidade
Testes de Carcinogenicidade
Galinhas
Fibrossarcoma/virologia
Vírus Auxiliares
Organismos Livres de Patógenos Específicos
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oncogene Proteins)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170126
[Lr] Data última revisão:
170126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160923
[St] Status:MEDLINE


  7 / 1394 MEDLINE  
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[PMID]:27387973
[Au] Autor:Dziewit L; Radlinska M
[Ad] Endereço:Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
[Ti] Título:Two Inducible Prophages of an Antarctic Pseudomonas sp. ANT_H14 Use the Same Capsid for Packaging Their Genomes - Characterization of a Novel Phage Helper-Satellite System.
[So] Source:PLoS One;11(7):e0158889, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Two novel prophages ФAH14a and ФAH14b of a psychrotolerant Antarctic bacterium Pseudomonas sp. ANT_H14 have been characterized. They were simultaneously induced with mitomycin C and packed into capsids of the same size and protein composition. The genome sequences of ФAH14a and ФAH14b have been determined. ФAH14b, the phage with a smaller genome (16,812 bp) seems to parasitize ФAH14a (55,060 bp) and utilizes its capsids, as only the latter encodes a complete set of structural proteins. Both viruses probably constitute a phage helper-satellite system, analogous to the P2-P4 duo. This study describes the architecture and function of the ФAH14a and ФAH14b genomes. Moreover, a functional analysis of a ФAH14a-encoded lytic enzyme and a DNA methyltransferase was performed. In silico analysis revealed the presence of the homologs of ФAH14a and ФAH14b in other Pseudomonas genomes, which may suggest that helper-satellite systems related to the one described in this work are common in pseudomonads.
[Mh] Termos MeSH primário: Bacteriófagos/genética
Prófagos/genética
Pseudomonas/virologia
Vírus Satélites/genética
[Mh] Termos MeSH secundário: Regiões Antárticas
Capsídeo/metabolismo
Proteínas do Capsídeo/genética
DNA (Citosina-5-)-Metiltransferases/genética
Análise Mutacional de DNA
DNA Viral/metabolismo
Regulação Viral da Expressão Gênica
Genes Virais
Vírus Auxiliares/genética
Microbiologia do Solo
Ativação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (DNA, Viral); EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160709
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0158889


  8 / 1394 MEDLINE  
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[PMID]:27367841
[Au] Autor:Rastall DP; Seregin SS; Aldhamen YA; Kaiser LM; Mullins C; Liou A; Ing F; Pereria-Hicks C; Godbehere-Roosa S; Palmer D; Ng P; Amalfitano A
[Ad] Endereço:Department of Microbiology and Molecular Genetics, College of Osteopathic Medicine, Michigan State University, East Lansing, MI, USA.
[Ti] Título:Long-term, high-level hepatic secretion of acid α-glucosidase for Pompe disease achieved in non-human primates using helper-dependent adenovirus.
[So] Source:Gene Ther;23(10):743-752, 2016 Oct.
[Is] ISSN:1476-5462
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pompe disease (glycogen storage disease type II (GSD-II)) is a myopathy caused by a genetic deficiency of acid α-glucosidase (GAA) leading to lysosomal glycogen accumulation causing muscle weakness, respiratory insufficiency and death. We previously demonstrated in GSD-II mice that a single injection of a helper-dependent adenovirus (HD-Ad) expressing GAA resulted in at least 300 days of liver secretion of GAA, correction of the glycogen storage in cardiac and skeletal muscles and improved muscle strength. Recent reports suggest that gene therapy modeling for lysososomal storage diseases in mice fails to predict outcomes in larger animal models. We therefore evaluated an HD-Ad expressing GAA in non-human primates. The baboons not only tolerated the procedure well, but the results also confirmed that a single dose of the HD-Ad allowed the livers of the treated animals to express and secrete large amounts of GAA for at least 6 months, at levels similar to those achieved in mice. Moreover, we detected liver-derived GAA in the heart, diaphragm and skeletal muscles of the treated animals for the duration of the study at levels that corrected glycogen accumulation in mice. This work validates our proof-of-concept studies in mice, and justifies future efforts using Ad-based vectors in Pompe disease patients.
[Mh] Termos MeSH primário: Adenoviridae/genética
Terapia Genética/métodos
Doença de Depósito de Glicogênio Tipo II/terapia
Fígado/secreção
alfa-Glucosidases/genética
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Diafragma/metabolismo
Terapia Genética/efeitos adversos
Vetores Genéticos/efeitos adversos
Vetores Genéticos/genética
Vírus Auxiliares/genética
Fígado/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Músculo Esquelético/metabolismo
Miocárdio/metabolismo
Papio
alfa-Glucosidases/metabolismo
alfa-Glucosidases/secreção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.2.1.20 (alpha-Glucosidases)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170724
[Lr] Data última revisão:
170724
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160702
[St] Status:MEDLINE
[do] DOI:10.1038/gt.2016.53


  9 / 1394 MEDLINE  
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[PMID]:27193570
[Au] Autor:Eini O; Behjatnia SA
[Ad] Endereço:Department of Plant Protection, School of Agriculture, University of Zanjan, Zanjan, Iran. omid.eini@znu.ac.ir.
[Ti] Título:The minimal sequence essential for replication and movement of Cotton leaf curl Multan betasatellite DNA by a helper virus in plant cells.
[So] Source:Virus Genes;52(5):679-87, 2016 Oct.
[Is] ISSN:1572-994X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Betasatellites are single-stranded circular DNAs associated with a number of monopartite begomoviruses. Betasatellites rely on the helper begomoviruses for replication and movement in plant tissues and plant-to-plant transmission by vectors. Their genomes are approximately half the size of the helper viruses and consist of three main regions including the ßC1 gene, an adenine-rich (A-rich) region, and the satellite conserved region (SCR). In this study, we investigated the minimal sequences required for Cotton leaf curl Multan betasatellite (CLCuMB) replication and movement. Mutational analysis of CLCuMB DNA genome indicated that ßC1 gene and A-rich region were not required for trans-replication and movement of CLCuMB in host plants by a helper virus. Deletion of ßC1 gene and a fragment (135 nt in length) upstream of this gene impaired CLCuMB replication. However, CLCuMB mutant with deletion of ßC1 gene and a further 163 nucleotides replicated at a lower level as compared to the wild-type betasatellite. This suggests that there are essential elements in the fragment upstream of ßC1 gene, which are required for the replication of CLCuMB rather than the size limitation of CLCuMB DNA.
[Mh] Termos MeSH primário: Begomovirus/genética
Replicação do DNA/genética
DNA Satélite/genética
Gossypium/virologia
Vírus Auxiliares/genética
Células Vegetais/virologia
Folhas de Planta/virologia
[Mh] Termos MeSH secundário: Análise Mutacional de DNA/métodos
DNA Viral/genética
Genoma Viral/genética
Mutação/genética
Doenças das Plantas/virologia
Vírus de Plantas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Satellite); 0 (DNA, Viral)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160520
[St] Status:MEDLINE
[do] DOI:10.1007/s11262-016-1354-6


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[PMID]:27179165
[Au] Autor:Ajorloo M; Bamdad T; Gholami AR; Azadmanesh K
[Ad] Endereço:Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
[Ti] Título:Assessment the Efficiency of the Constructed Minigenome of Rabies Virus using PV Strain as Helper Virus.
[So] Source:Arch Iran Med;19(5):335-41, 2016 May.
[Is] ISSN:1735-3947
[Cp] País de publicação:Iran
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Rabies is an acute viral disease that causes encephalomyelitis in mammals and human. The only way to prevent this disease is through vaccination before or after exposure. The aim of this study is to evaluate the efficiency of the Pasteur virus (PV) minigenome, using PV strain. MATERIALS AND METHODS: Enhanced Green Fluorescent Protein (EGFP) sequence was placed between the designed necessary elements (Hammerhead, HDV ribozyme, 3' Leader, and 5' Trailer sequences), which resemble the rabies virus PV strain (PV2061) genome and anti-genome. These constructs were placed between T7 polymerase promoter and T7 polymerase terminator sequences. The accuracy of the minigenome was confirmed by the expression of EGFP using the helper virus in T7-BHK cell line. RESULTS: The viral necessary elements of positive and negative sense strands were evaluated for the ability of EGFP expression in the presence of the helper virus. While the positive strand showed background results, no EGFP background was observed in the negative strand application. CONCLUSION: Establishment of minigenome system does not require advanced biosafety levels. Furthermore, using minigenome system eliminates many potential confounding factors that may be present in coding regions of the genome. Use of the minigenome system is easier and more feasible than the full genome rescue of the virus. This study successfully shows the efficiency of the constructed rabies virus minigenome in expression of inserted gene.
[Mh] Termos MeSH primário: Genoma Viral
Vírus Auxiliares/genética
RNA Viral/genética
Vírus da Raiva/genética
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Cricetinae
Proteínas de Fluorescência Verde/genética
Regiões Promotoras Genéticas
Raiva/prevenção & controle
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral); 0 (enhanced green fluorescent protein); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160515
[St] Status:MEDLINE
[do] DOI:0161905/AIM.007



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