Base de dados : MEDLINE
Pesquisa : B04.525 [Categoria DeCS]
Referências encontradas : 2003 [refinar]
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  1 / 2003 MEDLINE  
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[PMID]:29058655
[Au] Autor:Bigot D; Dalmon A; Roy B; Hou C; Germain M; Romary M; Deng S; Diao Q; Weinert LA; Cook JM; Herniou EA; Gayral P
[Ad] Endereço:1​Institut de Recherche sur la Biologie de l'Insecte, UMR 7261, CNRS, Université de Tours, 37200 Tours, France.
[Ti] Título:The discovery of Halictivirus resolves the Sinaivirus phylogeny.
[So] Source:J Gen Virol;98(11):2864-2875, 2017 Nov.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:By providing pollination services, bees are among the most important insects, both in ecological and economical terms. Combined next-generation and classical sequencing approaches were applied to discover and study new insect viruses potentially harmful to bees. A bioinformatics virus discovery pipeline was used on individual Illumina transcriptomes of 13 wild bees from three species from the genus Halictus and 30 ants from six species of the genera Messor and Aphaenogaster. This allowed the discovery and description of three sequences of a new virus termed Halictus scabiosae Adlikon virus (HsAV). Phylogenetic analyses of ORF1, RNA-dependent RNA-polymerase (RdRp) and capsid genes showed that HsAV is closely related to (+)ssRNA viruses of the unassigned Sinaivirus genus but distant enough to belong to a different new genus we called Halictivirus. In addition, our study of ant transcriptomes revealed the first four sinaivirus sequences from ants (Messor barbarus, M. capitatus and M. concolor). Maximum likelihood phylogenetic analyses were performed on a 594 nt fragment of the ORF1/RdRp region from 84 sinaivirus sequences, including 31 new Lake Sinai viruses (LSVs) from honey bees collected in five countries across the globe and the four ant viral sequences. The phylogeny revealed four main clades potentially representing different viral species infecting honey bees. Moreover, the ant viruses belonged to the LSV4 clade, suggesting a possible cross-species transmission between bees and ants. Lastly, wide honey bee screening showed that all four LSV clades have worldwide distributions with no obvious geographical segregation.
[Mh] Termos MeSH primário: Formigas/virologia
Abelhas/virologia
Vírus dos Insetos/classificação
Vírus dos Insetos/isolamento & purificação
Filogenia
[Mh] Termos MeSH secundário: Animais
Variação Genética
Vírus dos Insetos/genética
Análise de Sequência de DNA
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171024
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000957


  2 / 2003 MEDLINE  
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[PMID]:29048274
[Au] Autor:Kobayashi D; Isawa H; Fujita R; Murota K; Itokawa K; Higa Y; Katayama Y; Sasaki T; Mizutani T; Iwanaga S; Ohta N; Garcia-Bertuso A; Sawabe K
[Ad] Endereço:1​Department of Environmental Parasitology, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
[Ti] Título:Isolation and characterization of a new iflavirus from Armigeres spp. mosquitoes in the Philippines.
[So] Source:J Gen Virol;98(11):2876-2881, 2017 Nov.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:During an entomological surveillance for arthropod-borne viruses in the Philippines, we isolated a previously unrecognized virus from female Armigeres spp. mosquitoes. Whole-genome sequencing, genetic characterization and phylogenetic analysis revealed that the isolated virus, designated Armigeres iflavirus (ArIFV), is a novel member of the iflaviruses (genus Iflavirus, family Iflaviridae) and phylogenetically related to Moku virus, Hubei odonate virus 4, slow bee paralysis virus and Graminella nigrifrons virus 1. To our knowledge, this is the first successful isolation of iflavirus from a dipteran insect. Spherical ArIFV particles of approximately 30 nm in diameter contained at least three major structural proteins. ArIFV multiplied to high titres (~10 p.f.u. ml ) and formed clear plaques in a mosquito cell line, C6/36. Our findings provide new insights into the infection mechanism, genetic diversity and evolution of the Iflaviridae family.
[Mh] Termos MeSH primário: Culicidae/virologia
Vírus dos Insetos/classificação
Vírus dos Insetos/isolamento & purificação
Vírus de RNA/classificação
Vírus de RNA/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Filipinas
Ensaio de Placa Viral
Proteínas Estruturais Virais/análise
Vírion/química
Vírion/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Structural Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171020
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000929


  3 / 2003 MEDLINE  
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[PMID]:28973572
[Au] Autor:Grau T; Brandt A; DeLeon S; Meixner MD; Strauß JF; Joop G; Telschow A
[Ad] Endereço:Institute of Insect Biotechnology, Justus-Liebig University Giessen, Giessen, Germany.
[Ti] Título:A Comparison of Wolbachia Infection Frequencies in Varroa With Prevalence of Deformed Wing Virus.
[So] Source:J Insect Sci;17(3), 2017 May 01.
[Is] ISSN:1536-2442
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Wolbachia are widely distributed bacterial endosymbionts of arthropods and filarial nematodes. These bacteria can affect host fitness in a variety of ways, such as protecting hosts against viruses and other pathogens. Here, we investigate the possible role of Wolbachia in the prevalence of the deformed wing virus (DWV), a highly virulent pathogen of honey bees (Apis mellifera) that is transmitted by parasitic Varroa mites (Varroa destructor). About 180 Varroa mites from 18 beehives were tested for infection with Wolbachia and DWV. We first screened for Wolbachia using two standard primers (wsp and 16S rDNA), and found 26% of the mites to be positive for Wolbachia using the wsp primer and 64% of the mites to be positive using the 16S rDNA primer. Using these intermediate Wolbachia frequencies, we then tested for statistical correlations with virus infection frequencies. The analysis revealed a significant positive correlation between DWV and Wolbachia using the wsp primer, but no significant association between DWV and Wolbachia using the 16S rDNA primer. In conclusion, there is no evidence for an anti-pathogenic effect of Wolbachia in V. destructor, but weak evidence for a pro-pathogenic effect. These results encourage further examination of Wolbachia-virus interactions in Varroa mites since an increased vector competence of the mites may significantly impact disease outbreaks in honey bees.
[Mh] Termos MeSH primário: Vírus dos Insetos/isolamento & purificação
Varroidae/virologia
Wolbachia/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE
[do] DOI:10.1093/jisesa/iex039


  4 / 2003 MEDLINE  
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[PMID]:28940716
[Au] Autor:Dong SM; Cui JH; Zhang W; Zhang XW; Kou TC; Cai QC; Xu S; You S; Yu DS; Ding L; Lai JH; Li M; Luo KJ
[Ad] Endereço:Key Laboratory for Animal Genetic Diversity and Evolution of High Education in Yunnan Province, School of Life Sciences, Yunnan University, Kunming, P.R. China.
[Ti] Título:Inhibition of translation initiation factor eIF4A is required for apoptosis mediated by Microplitis bicoloratus bracovirus.
[So] Source:Arch Insect Biochem Physiol;96(3), 2017 Nov.
[Is] ISSN:1520-6327
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Apoptotic hemocytes induced by Microplitis bicoloratus parasitism have been reported, and M. bicoloratus bracovirus (MbBV) is known to be the apoptosis inducer. However, the mechanism how MbBV regulates apoptosis remains unclear. eIF4A, one of translation initiation factors, was found from a Spodoptera litura transcriptome, the expression of which in the parasitized hemocytes of S. litura was inhibited in RT-qPCR analysis. The western blot also illustrated eIF4A at 6-day post-parasitization was inhibited in hemocytes. For testing interaction of MbBV-eIF4A-apoptosis, a cDNA clone encoding 1,266 bp of eIF4A was obtained from S. litura hemocytes and sequenced. Then, a 48 kDa V5-fusion protein of the eIF4A was detected by using the anti-V5 antibody at 72-h post-transfection in the High Five cells, which is located in the cell cytoplasm. In vitro, overexpression of eIF4A rescued the apoptotic High Five cells induced by MbBV. Conversely, in vivo, loss of eIF4A proteins by dsRNA feeding increased apoptosis of hemocytes. Furthermore, RNAi and parasitism significantly increased apoptosis of hemocytes in S. litura. These findings suggested that MbBV inhibited the expression of eIF4A, which was required for apoptosis mediated by MbBV. This study will contribute to biological pest control and enhance our understanding of molecular mechanisms underlying polydnavirus-parasitoid-host interaction.
[Mh] Termos MeSH primário: Apoptose/fisiologia
Fatores de Iniciação em Eucariotos/metabolismo
Hemócitos/metabolismo
Vírus dos Insetos/fisiologia
Mariposas/virologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Fatores de Iniciação em Eucariotos/genética
Regulação da Expressão Gênica
Interações Hospedeiro-Patógeno
Proteínas de Insetos/genética
Proteínas de Insetos/metabolismo
Mariposas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Eukaryotic Initiation Factors); 0 (Insect Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170924
[St] Status:MEDLINE
[do] DOI:10.1002/arch.21423


  5 / 2003 MEDLINE  
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[PMID]:28857036
[Au] Autor:Olendraite I; Lukhovitskaya NI; Porter SD; Valles SM; Firth AE
[Ad] Endereço:1​Division of Virology, Department of Pathology, University of Cambridge, Cambridge, CB2 1QP, UK.
[Ti] Título:Polycipiviridae: a proposed new family of polycistronic picorna-like RNA viruses.
[So] Source:J Gen Virol;98(9):2368-2378, 2017 Sep.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Solenopsis invicta virus 2 is a single-stranded positive-sense picorna-like RNA virus with an unusual genome structure. The monopartite genome of approximately 11 kb contains four open reading frames in its 5' third, three of which encode proteins with homology to picornavirus-like jelly-roll fold capsid proteins. These are followed by an intergenic region, and then a single long open reading frame that covers the 3' two-thirds of the genome. The polypeptide translation of this 3' open reading frame contains motifs characteristic of picornavirus-like helicase, protease and RNA-dependent RNA polymerase domains. An inspection of public transcriptome shotgun assembly sequences revealed five related apparently nearly complete virus genomes isolated from ant species and one from a dipteran insect. By high-throughput sequencing and in silico assembly of RNA isolated from Solenopsis invicta and four other ant species, followed by targeted Sanger sequencing, we obtained nearly complete genomes for four further viruses in the group. Four further sequences were obtained from a recent large-scale invertebrate virus study. The 15 sequences are highly divergent (pairwise amino acid identities of as low as 17 % in the non-structural polyprotein), but possess the same overall polycistronic genome structure, which is distinct from all other characterized picorna-like viruses. Consequently, we propose the formation of a new virus family, Polycipiviridae, to classify this clade of arthropod-infecting polycistronic picorna-like viruses. We further propose that this family be divided into three genera: Chipolycivirus (2 species), Hupolycivirus (2 species) and Sopolycivirus (11 species), with members of the latter infecting ants in at least 3 different subfamilies.
[Mh] Termos MeSH primário: Formigas/virologia
Picornaviridae/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Genoma Viral
Vírus dos Insetos/classificação
Vírus dos Insetos/genética
Vírus dos Insetos/isolamento & purificação
Filogenia
Picornaviridae/classificação
Picornaviridae/genética
RNA Viral/genética
RNA Viral/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171115
[Lr] Data última revisão:
171115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000902


  6 / 2003 MEDLINE  
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[PMID]:28719335
[Au] Autor:Contreras-Gutierrez MA; Guzman H; Thangamani S; Vasilakis N; Tesh RB
[Ad] Endereço:Grupo de Investigacion en Sistematica Molecular (GSM), Facultad de Ciencias, Universidad Nacional de Colombia, Medellin, Colombia.
[Ti] Título:Experimental Infection with and Maintenance of Cell Fusing Agent Virus ( ) in .
[So] Source:Am J Trop Med Hyg;97(1):299-304, 2017 Jul.
[Is] ISSN:1476-1645
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:During the past two decades, there has been a dramatic increase in the recognition and characterization of novel insect-specific flaviviruses (ISFVs). Some of these agents are closely related to important mosquito-borne flavivirus pathogens. Results of experimental studies suggest that mosquitoes and mosquito cell cultures infected with some ISFVs are refractory to superinfection with related flavivirus pathogens; and it has been proposed that ISFVs potentially could be used to alter the vector competence of mosquitoes and reduce transmission of specific flavivirus pathogens, such as dengue, West Nile, or Zika viruses. In order for an ISFV to be used in such a control strategy, the virus would have to be vertically transmitted at a high rate in the target vector population to insure its continued maintenance. This study compared the vertical transmission rates of an ISFV, cell fusing agent virus (CFAV), in two colonies: one naturally infected with CFAV and the other experimentally infected but previously free of the virus. CFAV filial infection rates in progeny of female mosquitoes from both colonies were > 90% after two generations of selection, indicating the feasibility of introducing an ISFV into a mosquito population. This and other considerations for evaluating the feasibility of using ISFVs as an arbovirus control strategy are discussed.
[Mh] Termos MeSH primário: Aedes/virologia
Flavivirus/fisiologia
Vírus dos Insetos/fisiologia
[Mh] Termos MeSH secundário: Animais
Feminino
Transmissão Vertical de Doença Infecciosa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE
[do] DOI:10.4269/ajtmh.16-0987


  7 / 2003 MEDLINE  
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[PMID]:28637756
[Au] Autor:Shi M; Neville P; Nicholson J; Eden JS; Imrie A; Holmes EC
[Ad] Endereço:Marie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Biological Sciences and Sydney Medical School, The University of Sydney, Sydney, Australia.
[Ti] Título:High-Resolution Metatranscriptomics Reveals the Ecological Dynamics of Mosquito-Associated RNA Viruses in Western Australia.
[So] Source:J Virol;91(17), 2017 Sep 01.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mosquitoes harbor a high diversity of RNA viruses, including many that impact human health. Despite a growing effort to describe the extent and nature of the mosquito virome, little is known about how these viruses persist, spread, and interact with both their hosts and other microbes. To address this issue we performed a metatranscriptomics analysis of 12 Western Australian mosquito populations structured by species and geographic location. Our results identified the complete genomes of 24 species of RNA viruses from a diverse range of viral families and orders, among which 19 are newly described. Comparisons of viromes revealed a striking difference between the two mosquito genera, with viromes of mosquitoes of the genus exhibiting substantially less diversity and lower abundances than those of mosquitoes of the genus, within which the viral abundance reached 16.87% of the total non-rRNA. In addition, there was little overlap in viral diversity between the two genera, although the viromes were very similar among the three species studied, suggesting that the host taxon plays a major role in structuring virus diversity. In contrast, we found no evidence that geographic location played a major role in shaping RNA virus diversity, and several viruses discovered here exhibited high similarity (95 to 98% nucleotide identity) to those from Indonesia and China. Finally, using abundance-level and phylogenetic relationships, we were able to distinguish potential mosquito viruses from those present in coinfecting bacteria, fungi, and protists. In sum, our metatranscriptomics approach provides important insights into the ecology of mosquito RNA viruses. Studies of virus ecology have generally focused on individual viral species. However, recent advances in bulk RNA sequencing make it possible to utilize metatranscriptomic approaches to reveal both complete virus diversity and the relative abundance of these viruses. We used such a metatranscriptomic approach to determine key aspects of the ecology of mosquito viruses in Western Australia. Our results show that RNA viruses are some of the most important components of the mosquito transcriptome, and we identified 19 new virus species from a diverse set of virus families. A key result was that host genetic background plays a more important role in shaping virus diversity than sampling location, with species harboring more viruses at higher abundance than those from mosquitoes.
[Mh] Termos MeSH primário: Aedes/virologia
Culex/virologia
Vírus dos Insetos/genética
Vírus de RNA/genética
RNA Viral/genética
[Mh] Termos MeSH secundário: Animais
Ecologia
Variação Genética
Genoma Viral
Filogenia
Análise de Sequência de RNA
Transcriptoma
Austrália Ocidental
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170902
[Lr] Data última revisão:
170902
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE


  8 / 2003 MEDLINE  
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[PMID]:28628366
[Au] Autor:Newman CM; Krebs BL; Anderson TK; Hamer GL; Ruiz MO; Brawn JD; Brown WM; Kitron UD; Goldberg TL
[Ad] Endereço:1 Department of Pathology and Laboratory Medicine, School of Medicine and Public Health, University of Wisconsin-Madison , Madison, Wisconsin.
[Ti] Título:Culex Flavivirus During West Nile Virus Epidemic and Interepidemic Years in Chicago, United States.
[So] Source:Vector Borne Zoonotic Dis;17(8):567-575, 2017 Aug.
[Is] ISSN:1557-7759
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Culex flavivirus (CxFV) is an insect-specific flavivirus infecting Culex mosquitoes, which are important vectors of West Nile virus (WNV). CxFV and WNV cocirculate in nature and coinfect Culex mosquitoes, including in a WNV "hotspot" in suburban Chicago. We previously identified a positive association between CxFV and WNV in mosquito pools collected from suburban Chicago in 2006. To further investigate this phenomenon, we compared the spatial and temporal distribution of CxFV during an interepidemic year (2011) and an epidemic year (2012) for WNV. Both viruses were more prevalent in mosquito pools in 2012 compared to 2011. During both years, the CxFV infection status of mosquito pools was associated with environmental factors such as habitat type and precipitation frequency rather than coinfection with WNV. These results support the idea that WNV and CxFV are ecologically associated, perhaps because both viruses respond to similar environmental drivers of mosquito populations.
[Mh] Termos MeSH primário: Culex/virologia
Epidemias
Vírus dos Insetos/isolamento & purificação
Febre do Nilo Ocidental/epidemiologia
Vírus do Nilo Ocidental/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Chicago/epidemiologia
Fatores de Tempo
Zoonoses
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.1089/vbz.2017.2124


  9 / 2003 MEDLINE  
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[PMID]:28432563
[Au] Autor:Li B; Deng S; Yang D; Hou C; Diao Q
[Ad] Endereço:Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, No. 1 Beigou, Xiangshan, Haidian District, Beijing, 100093, People's Republic of China.
[Ti] Título:Complete sequences of the RNA 1 and RNA 2 segments of chronic bee paralysis virus strain CBPV-BJ detected in China.
[So] Source:Arch Virol;162(8):2451-2456, 2017 Aug.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Chronic bee paralysis virus (CBPV) is an important viral pathogen that affects adult bees. Although several CBPV strains have been reported, little information has been obtained from China. In this study, two major segments of the CBPV Chinese isolate CBPV-BJ, RNA 1 and RNA 2, were determined to be 3657 and 2267 nucleotides (nt) in length, respectively. RNA 1 and RNA 2 contained three and four open reading frames (ORFs), respectively, which agreed with known reference strains (EU122229 and EU122230). The RNA 1 had 98% nucleotide sequence identity to a known Chinese strain (KU950353), and RNA 2 had 97% nucleotide sequence identity to another Chinese strain (KU950354). Although the lengths of the RNA 1 and RNA 2 sequences were 17 nt and 38 nt shorter than those of the CBPV reference strains EU122229 and EU122230, respectively, the complete CBPV-BJ RNA 1 and RNA 2 sequences shared 91% and 92% identity with them. Phylogenetic analysis based on the sequences of the RNA-dependent RNA polymerase (RdRp) and putative structural proteins (pSPs) showed that CBPV-BJ was most closely related to the other two Chinese isolate (KU950353 and KU950354) and clustered with most Asian strains. These data provide new information that will lead to a better understanding of the diversity of the CBPV genome.
[Mh] Termos MeSH primário: Abelhas/virologia
Vírus dos Insetos/genética
Vírus de RNA/genética
RNA Viral/genética
[Mh] Termos MeSH secundário: Animais
China
Variação Genética
Genoma Viral
Vírus dos Insetos/isolamento & purificação
Vírus dos Insetos/patogenicidade
Fases de Leitura Aberta
Vírus de RNA/isolamento & purificação
Análise de Sequência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170423
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3373-6


  10 / 2003 MEDLINE  
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[PMID]:28429131
[Au] Autor:Ghorani M; Madadgar O; Langeroudi AG; Rezapanah M; Nabian S; Akbarein H; Farahani RK; Maghsoudloo H; Abdollahi H; Forsi M
[Ad] Endereço:Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
[Ti] Título:The first comprehensive molecular detection of six honey bee viruses in Iran in 2015-2016.
[So] Source:Arch Virol;162(8):2287-2291, 2017 Aug.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:At least 18 viruses have been reported in the honey bee (Apis mellifera L.). However, severe diseases in honey bees are mainly caused by six viruses, and these are the most important in beekeeping. These viruses include: deformed wing virus (DWV), acute bee paralysis virus (ABPV), chronic bee paralysis virus (CBPV), sacbrood virus (SBV), kashmir bee virus (KBV), and black queen cell virus (BQCV). In this study, we evaluated 89 Iranian honey bee apiaries (during the period 2015-2016) suffering from symptoms of depopulation, sudden collapse, paralysis, or dark coloring, by employing reverse transcription-PCR. Samples were collected from four regions (Mazandaran, Hormozgan, Kurdistan, and Khorasan Razavi) of Iran. Of the 89 apiaries examined, 16 (17.97%), three (3.37%), and three (3.37%) were infected by DWV, ABPV, and CBPV, respectively. The study results for the other viruses (SBV, KBV, and BQCV) were negative. The present study evaluated the presence of the six most important honey bee viruses in bee colonies with suspected infections, and identified remarkable differences in the distribution patterns of the viruses in different geographic regions of Iran.
[Mh] Termos MeSH primário: Abelhas/virologia
Vírus dos Insetos/classificação
[Mh] Termos MeSH secundário: Animais
Vírus dos Insetos/genética
Vírus dos Insetos/isolamento & purificação
Irã (Geográfico)
RNA Viral/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170422
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3370-9



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde