Base de dados : MEDLINE
Pesquisa : B04.613.807.070 [Categoria DeCS]
Referências encontradas : 1029 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 103 ir para página                         

  1 / 1029 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29244184
[Au] Autor:Senigl F; Miklík D; Auxt M; Hejnar J
[Ad] Endereço:Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, CZ-14220 Prague 4, Czech Republic.
[Ti] Título:Accumulation of long-term transcriptionally active integrated retroviral vectors in active promoters and enhancers.
[So] Source:Nucleic Acids Res;45(22):12752-12765, 2017 Dec 15.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Most retroviruses preferentially integrate into certain genomic locations and, as a result, their genome-wide integration patterns are non-random. We investigate the epigenetic landscape of integrated retroviral vectors and correlate it with the long-term stability of proviral transcription. Retroviral vectors derived from the avian sarcoma/leukosis virus expressing the GFP reporter were used to transduce the human myeloid lymphoblastoma cell line K562. Because of efficient silencing of avian retrovirus in mammalian cells, only ∼3% of established clones displayed stable proviral expression. We analyzed the vector integration sites in non-selected cells and in clones selected for the GFP expression. This selection led to overrepresentation of proviruses integrated in active transcription units, with particular accumulation in promoter-proximal areas. In parallel, we investigated the integration of vectors equipped with an anti-silencing CpG island core sequence. Such modification increased the frequency of stably expressing proviruses by one order. The modified vectors are also overrepresented in active transcription units, but stably expressed in distal parts of transcriptional units further away from promoters with marked accumulation in enhancers. These results suggest that integrated retroviruses subject to gradual epigenetic silencing during long-term cultivation. Among most genomic compartments, however, active promoters and enhancers protect the adjacent retroviruses from transcriptional silencing.
[Mh] Termos MeSH primário: Alpharetrovirus/genética
Elementos Facilitadores Genéticos/genética
Regiões Promotoras Genéticas/genética
Transcrição Genética
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Ilhas de CpG/genética
Epigênese Genética
Inativação Gênica
Vetores Genéticos/genética
Seres Humanos
Células K562
Provírus/genética
Integração Viral/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx889


  2 / 1029 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27138041
[Au] Autor:Hübner J; Hoseini SS; Suerth JD; Hoffmann D; Maluski M; Herbst J; Maul H; Ghosh A; Eiz-Vesper B; Yuan Q; Ott M; Heuser M; Schambach A; Sauer MG
[Ad] Endereço:Department of Pediatric Hematology and Oncology, Hannover Medical School, Hannover, Germany.
[Ti] Título:Generation of Genetically Engineered Precursor T-Cells From Human Umbilical Cord Blood Using an Optimized Alpharetroviral Vector Platform.
[So] Source:Mol Ther;24(7):1216-26, 2016 Aug.
[Is] ISSN:1525-0024
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Retroviral engineering of hematopoietic stem cell-derived precursor T-cells (preTs) opens the possibility of targeted T-cell transfer across human leukocyte antigen (HLA)-barriers. Alpharetroviral vectors exhibit a more neutral integration pattern thereby reducing the risk of insertional mutagenesis. Cord blood-derived CD34+ cells were transduced and differentiated into preTs in vitro. Two promoters, elongation-factor-1-short-form, and a myeloproliferative sarcoma virus variant in combination with two commonly used envelopes were comparatively assessed choosing enhanced green fluorescent protein or a third-generation chimeric antigen receptor (CAR) against CD123 as gene of interest. Furthermore, the inducible suicide gene iCaspase 9 has been validated. Combining the sarcoma virus-derived promoter with a modified feline endogenous retrovirus envelope glycoprotein yielded in superior transgene expression and transduction rates. Fresh and previously frozen CD34+ cells showed similar transduction and expansion rates. Transgene-positive cells did neither show proliferative impairment nor alteration in their lymphoid differentiation profile. The sarcoma virus-derived promoter only could express sufficient levels of iCaspase 9 to mediate dimerizer-induced apoptosis. Finally, the CD123 CAR was efficiently expressed in CD34+ cells and proved to be functional when expressed on differentiated T-cells. Therefore, the transduction of CD34+ cells with alpharetroviral vectors represents a feasible and potentially safer approach for stem cell-based immunotherapies for cancer.
[Mh] Termos MeSH primário: Alpharetrovirus/genética
Sangue Fetal/citologia
Engenharia Genética
Vetores Genéticos/genética
Células Precursoras de Linfócitos T/citologia
Células Precursoras de Linfócitos T/metabolismo
[Mh] Termos MeSH secundário: Antígenos CD34/metabolismo
Apoptose
Proteínas da Membrana Bacteriana Externa
Biomarcadores
Diferenciação Celular
Expressão Gênica
Técnicas de Transferência de Genes
Genes Reporter
Células-Tronco Hematopoéticas/citologia
Células-Tronco Hematopoéticas/metabolismo
Seres Humanos
Subunidade alfa de Receptor de Interleucina-3/imunologia
Fenótipo
Regiões Promotoras Genéticas
Receptores de Antígenos de Linfócitos T/genética
Receptores de Antígenos de Linfócitos T/imunologia
Linfócitos T/citologia
Linfócitos T/imunologia
Linfócitos T/metabolismo
Transdução Genética
Transgenes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD34); 0 (Bacterial Outer Membrane Proteins); 0 (Biomarkers); 0 (Interleukin-3 Receptor alpha Subunit); 0 (Receptors, Antigen, T-Cell); 0 (membrane protein, 75kDa)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160504
[St] Status:MEDLINE
[do] DOI:10.1038/mt.2016.89


  3 / 1029 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26300042
[Au] Autor:Suerth JD; Morgan MA; Kloess S; Heckl D; Neudörfl C; Falk CS; Koehl U; Schambach A
[Ad] Endereço:Institute of Experimental Hematology, Hannover Medical School, 30625, Hannover, Germany.
[Ti] Título:Efficient generation of gene-modified human natural killer cells via alpharetroviral vectors.
[So] Source:J Mol Med (Berl);94(1):83-93, 2016 Jan.
[Is] ISSN:1432-1440
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: Natural killer (NK) cells play an important role in tumor immunotherapy with their unique capability of killing transformed cells without the need for prior sensitization and without major histocompatibility complex (MHC)/peptide restriction. However, tumor cells can escape NK cell cytotoxicity by various tumor immune escape mechanisms. To overcome these escape mechanisms, NK cells can be modified to express chimeric antigen receptors (CARs), enhancing their tumor-specific cytotoxicity. To determine the most efficacious method to modify human NK cells, we compared different retroviral vector systems, retroviral pseudotypes, and transduction protocols. Using optimized transduction conditions, the highest transduction levels (up to 60%) were achieved with alpharetroviral vectors. Alpharetroviral-modified primary human NK cells exhibited no alteration in receptor expression and had similar degranulation activity as untransduced NK cells, thus demonstrating that alpharetroviral modification did not negatively affect NK cell cytotoxicity. Transduction of NK cells with an alpharetroviral vector containing a CD19 CAR expression cassette selectively enhanced NK cell cytotoxicity towards CD19-expressing leukemia cells, achieving nearly complete elimination of leukemia cells after 48 h. Taken together, alpharetroviral vectors are promising tools for NK cell-mediated cancer immunotherapy applications. KEY MESSAGES: Efficient modification of human NK cells using alpharetroviral vectors. Anti-CD19-CAR-NK cells exhibited improved cytotoxicity towards CD19(+) leukemia cells. Alpharetroviral vectors are promising tools for immunotherapy applications using NK cells.
[Mh] Termos MeSH primário: Alpharetrovirus/genética
Antígenos CD19/genética
Citotoxicidade Imunológica/genética
Vetores Genéticos/genética
Células Matadoras Naturais/imunologia
Leucemia/terapia
Receptores de Antígenos/genética
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Citotoxicidade Imunológica/imunologia
Terapia Genética/métodos
Proteínas de Fluorescência Verde/genética
Seres Humanos
Imunoterapia Adotiva/métodos
Células Matadoras Naturais/citologia
Leucemia/imunologia
Receptores de Antígenos/biossíntese
Receptores de Antígenos/imunologia
Transdução Genética/métodos
Evasão Tumoral/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, CD19); 0 (Receptors, Antigen); 0 (enhanced green fluorescent protein); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:170916
[Lr] Data última revisão:
170916
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150825
[St] Status:MEDLINE
[do] DOI:10.1007/s00109-015-1327-6


  4 / 1029 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:26608344
[Au] Autor:Arnaud O; Meyer S; Vallin E; Beslon G; Gandrillon O
[Ad] Endereço:Centre de Génétique et de Physiologie Moléculaire et Cellulaire (CGPhiMC), CNRS UMR5534, Université de Lyon, Université Lyon 1, 69622, Lyon, France. arnaudophe@yahoo.fr.
[Ti] Título:Temperature-induced variation in gene expression burst size in metazoan cells.
[So] Source:BMC Mol Biol;16:20, 2015 Nov 25.
[Is] ISSN:1471-2199
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Gene expression is an inherently stochastic process, owing to its dynamic molecular nature. Protein amount distributions, which can be acquired by cytometry using a reporter gene, can inform about the mechanisms of the underlying microscopic molecular system. RESULTS: By using different clones of chicken erythroid progenitor cells harboring different integration sites of a CMV-driven mCherry protein, we investigated the dynamical behavior of such distributions. We show that, on short term, clone distributions can be quickly regenerated from small population samples with a high accuracy. On longer term, on the contrary, we show variations manifested by correlated fluctuation in the Mean Fluorescence Intensity. In search for a possible cause of this correlation, we demonstrate that in response to small temperature variations cells are able to adjust their gene expression rate: a modest (2 °C) increase in external temperature induces a significant down regulation of mean expression values, with a reverse effect observed when the temperature is decreased. Using a two-state model of gene expression we further demonstrate that temperature acts by modifying the size of transcription bursts, while the burst frequency of the investigated promoter is less systematically affected. CONCLUSIONS: For the first time, we report that transcription burst size is a key parameter for gene expression that metazoan cells from homeotherm animals can modify in response to an external thermal stimulus.
[Mh] Termos MeSH primário: Eritroblastos/metabolismo
Células Precursoras Eritroides/metabolismo
Regulação da Expressão Gênica/fisiologia
Expressão Gênica/genética
Temperatura Ambiente
[Mh] Termos MeSH secundário: Alpharetrovirus/genética
Animais
Linhagem Celular Transformada
Galinhas
Citometria de Fluxo
Fluorescência
Regulação da Expressão Gênica/genética
Genes Reporter/genética
Regiões Promotoras Genéticas/genética
RNA Mensageiro/genética
Processos Estocásticos
Transcrição Genética/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Messenger)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151128
[Lr] Data última revisão:
151128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151127
[St] Status:MEDLINE
[do] DOI:10.1186/s12867-015-0048-2


  5 / 1029 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26600497
[Au] Autor:Zhu F; Jie H; Lian L; Qu LJ; Hou ZC; Zheng JX; Chen SY; Yang N; Liu YP
[Ad] Endereço:Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, China.
[Ti] Título:Avian sarcoma and leukosis virus gag gene in the Anser anser domesticus genome.
[So] Source:Genet Mol Res;14(4):14379-86, 2015 Nov 19.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Endogenous retroviruses are regarded as ideal genetic markers for evolutionary analyses. Birds were some of the initial vertebrates found to contain endogenous retroviruses. However, few studies have investigated the presence and distribution of endogenous retroviruses in goose. In this study, we detected the avian sarcoma and leukosis virus gag gene in the genomic DNA of 8 Chinese native breeds using polymerase chain reaction method. The results indicated that a 1.2-kb avian sarcoma and leukosis virus gag sequence was integrated into all 8 goose breeds. The mean genetic pairwise distance was 0.918% among the investigated geese. To the best of our knowledge, this is the first report demonstrating the presence of the endogenous retroviruses in the domestic goose genome. The genetic structure should be further examined in the domestic goose.
[Mh] Termos MeSH primário: Alpharetrovirus/genética
Anseriformes/genética
Evolução Molecular
Produtos do Gene gag/genética
[Mh] Termos MeSH secundário: Animais
Anseriformes/virologia
Cruzamento
DNA Mitocondrial/genética
Produtos do Gene gag/isolamento & purificação
Genoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Mitochondrial); 0 (Gene Products, gag)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:151125
[Lr] Data última revisão:
151125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151125
[St] Status:MEDLINE
[do] DOI:10.4238/2015.November.18.1


  6 / 1029 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:25897755
[Au] Autor:Thomas JM; Allison AB; Holmes EC; Phillips JE; Bunting EM; Yabsley MJ; Brown JD
[Ad] Endereço:Southeastern Cooperative Wildlife Disease Study, Department of Population Health, 589 D.W. Brooks Drive, Wildlife Health Building, College of Veterinary Medicine, The University of Georgia, Athens, Georgia, United States of America; Daniel B. Warnell School of Forestry and Natural Resources, The Uni
[Ti] Título:Molecular Surveillance for Lymphoproliferative Disease Virus in Wild Turkeys (Meleagris gallopavo) from the Eastern United States.
[So] Source:PLoS One;10(4):e0122644, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lymphoproliferative disease virus (LPDV) is a poorly understood, oncogenic avian retrovirus of domestic turkeys that has historically been restricted to Europe and Israel. However, a recent study reported LPDV in multiple wild turkey diagnostic cases from throughout the eastern United States of America (USA). To better understand the distribution of LPDV in the eastern USA, we surveyed 1,164 reportedly asymptomatic hunter-harvested wild turkeys from 17 states for the presence of LPDV proviral DNA by PCR. In total, 564/1,164 (47%) turkeys were positive for LPDV. Wild turkeys from each state had a relatively high prevalence of LPDV, although statewide prevalence varied from 26 to 83%. Phylogenetic analysis revealed two major clades of LPDV in the USA, although one was at a low frequency suggesting restricted transmission, as well as significant clustering by state of isolation. To determine the best tissue to target for diagnostic purposes, liver, spleen, and bone marrow were tested from a subset of 15 hunter-harvested wild turkeys and 20 wild turkey diagnostic cases. Overall, bone marrow provided the highest level of detection for both hunter-harvested turkeys and diagnostic cases. The sensitivity of LPDV detection between tissues was not significantly different for diagnostic cases, but was for hunter-harvested birds. These results indicate that LPDV infection is common and widespread in wild turkey populations throughout the eastern USA, even without overt signs of disease.
[Mh] Termos MeSH primário: Alpharetrovirus/genética
Doenças das Aves/virologia
Transtornos Linfoproliferativos/veterinária
Provírus/genética
Infecções por Retroviridae/veterinária
Perus/virologia
[Mh] Termos MeSH secundário: Animais
Doenças das Aves/epidemiologia
Monitoramento Epidemiológico
Feminino
Genes Virais
Transtornos Linfoproliferativos/epidemiologia
Transtornos Linfoproliferativos/virologia
Masculino
Dados de Sequência Molecular
Filogenia
Prevalência
Infecções por Retroviridae/epidemiologia
Infecções por Retroviridae/virologia
Análise de Sequência de DNA
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1604
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150422
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0122644


  7 / 1029 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:25873518
[Au] Autor:Chen W; Liu Y; Li H; Chang S; Shu D; Zhang H; Chen F; Xie Q
[Ad] Endereço:College of Animal Science, South China Agricultural University &Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou, 510642, P. R. China.
[Ti] Título:Intronic deletions of tva receptor gene decrease the susceptibility to infection by avian sarcoma and leukosis virus subgroup A.
[So] Source:Sci Rep;5:9900, 2015 Apr 15.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The group of avian sarcoma and leukosis virus (ASLV) in chickens contains six highly related subgroups, A to E and J. Four genetic loci, tva, tvb, tvc and tvj, encode for corresponding receptors that determine the susceptibility to the ASLV subgroups. The prevalence of ASLV in hosts may have imposed strong selection pressure toward resistance to ASLV infection, and the resistant alleles in all four receptor genes have been identified. In this study, two new alleles of the tva receptor gene, tva(r5) and tva(r6), with similar intronic deletions were identified in Chinese commercial broilers. These natural mutations delete the deduced branch point signal within the first intron, disrupting mRNA splicing of the tva receptor gene and leading to the retention of intron 1 and introduction of premature TGA stop codons in both the longer and shorter tva isoforms. As a result, decreased susceptibility to subgroup A ASLV in vitro and in vivo was observed in the subsequent analysis. In addition, we identified two groups of heterozygous allele pairs which exhibited quantitative differences in host susceptibility to ASLV-A. This study demonstrated that defective splicing of the tva receptor gene can confer genetic resistance to ASLV subgroup A in the host.
[Mh] Termos MeSH primário: Alpharetrovirus/genética
Leucose Aviária/genética
Proteínas Aviárias/genética
Predisposição Genética para Doença
Íntrons
Receptores Virais/genética
Deleção de Sequência
[Mh] Termos MeSH secundário: Alelos
Alpharetrovirus/classificação
Processamento Alternativo
Animais
Leucose Aviária/virologia
Sequência de Bases
Galinhas
Frequência do Gene
Ordem dos Genes
Genótipo
Técnicas de Genotipagem
Dados de Sequência Molecular
Polimorfismo Genético
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Avian Proteins); 0 (Receptors, Virus); 0 (Tva receptor)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150416
[St] Status:MEDLINE
[do] DOI:10.1038/srep09900


  8 / 1029 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25740482
[Au] Autor:Kuo TH; Whited JL
[Ad] Endereço:Brigham Regenerative Medicine Center, Department of Orthopedic Surgery, Brigham & Women's Hospital, Cambridge, MA, USA.
[Ti] Título:Pseudotyped retroviruses for infecting axolotl.
[So] Source:Methods Mol Biol;1290:127-40, 2015.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The ability to introduce DNA elements into host cells and analyze the effects has revolutionized modern biology. Here we describe a protocol to generate Moloney murine leukemia virus (MMLV)-based, replication-incompetent pseudotyped retrovirus capable of infecting axolotls and incorporating genetic information into their genome. When pseudotyped with vesicular stomatitis virus (VSV)-G glycoprotein, the retroviruses can infect a broad range of proliferative axolotl cell types. However, if the retrovirus is pseudotyped with an avian sarcoma leukosis virus (ASLV)-A envelope protein, only axolotl cells experimentally manipulated to express the cognate tumor virus A (TVA) receptor can be targeted by infections. These strategies enable robust transgene expression over many cell divisions, cell lineage tracing, and cell subtype targeting for gene expression.
[Mh] Termos MeSH primário: Ambystoma mexicanum/virologia
Vírus da Leucemia Murina de Moloney/genética
Vírus da Leucemia Murina de Moloney/fisiologia
Transfecção/métodos
[Mh] Termos MeSH secundário: Alpharetrovirus/genética
Ambystoma mexicanum/embriologia
Animais
Extremidades/embriologia
Vetores Genéticos/genética
Células HEK293
Seres Humanos
Fases de Leitura Aberta/genética
Plasmídeos/genética
Carga Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1511
[Cu] Atualização por classe:150305
[Lr] Data última revisão:
150305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150306
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-2495-0_10


  9 / 1029 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:25604889
[Au] Autor:Mei M; Ye J; Qin A; Wang L; Hu X; Qian K; Shao H
[Ad] Endereço:1] Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No.12 East Wenhui Road, Yangzhou, Jiangsu. 225009, P. R. China [2] Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou. 225009, P. R. China.
[Ti] Título:Identification of novel viral receptors with cell line expressing viral receptor-binding protein.
[So] Source:Sci Rep;5:7935, 2015 Jan 21.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The viral cell receptors and infection can be blocked by the expression of the viral receptor-binding protein. Thus, the viral cell receptor is an attractive target for anti-viral strategies, and the identification of viral cell receptor is critical for better understanding and controlling viral disease. As a model system for viral entry and anti-retroviral approaches, avian sarcoma/leukosis virus (ASLV, including the A-J ten subgroups) has been studied intensively and many milestone discoveries have been achieved based on work with ASLV. Here, we used a DF1 cell line expressed viral receptor-binding protein to efficiently identify chicken Annexin A2 (chANXA2) as a novel receptor for retrovirus ALV-J (avian leukosis virus subgroup J). Our data demonstrate that antibodies or siRNA to chANXA2 significantly inhibited ALV-J infection and replication, and over-expression of chANXA2 permitted the entry of ALV-J into its non-permissible cells. Our findings have not only identified chANXA2 as a novel biomarker for anti-ALV-J, but also demonstrated that cell lines with the expression of viral receptor-binding protein could be as efficient tools for isolating functional receptors to identify novel anti-viral targets.
[Mh] Termos MeSH primário: Alpharetrovirus/metabolismo
Anexina A2/biossíntese
Proteínas Aviárias/biossíntese
Receptores Virais/biossíntese
[Mh] Termos MeSH secundário: Alpharetrovirus/genética
Animais
Anexina A2/genética
Proteínas Aviárias/genética
Galinhas
Células HEK293
Seres Humanos
Receptores Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Annexin A2); 0 (Avian Proteins); 0 (Receptors, Virus)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150128
[Lr] Data última revisão:
150128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150122
[St] Status:MEDLINE
[do] DOI:10.1038/srep07935


  10 / 1029 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:25490763
[Au] Autor:Suerth JD; Labenski V; Schambach A
[Ad] Endereço:Institute of Experimental Hematology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany. suerth.julia@mh-hannover.de.
[Ti] Título:Alpharetroviral vectors: from a cancer-causing agent to a useful tool for human gene therapy.
[So] Source:Viruses;6(12):4811-38, 2014 Dec 05.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Gene therapy using integrating retroviral vectors has proven its effectiveness in several clinical trials for the treatment of inherited diseases and cancer. However, vector-mediated adverse events related to insertional mutagenesis were also observed, emphasizing the need for safer therapeutic vectors. Paradoxically, alpharetroviruses, originally discovered as cancer-causing agents, have a more random and potentially safer integration pattern compared to gammaretro- and lentiviruses. In this review, we provide a short overview of the history of alpharetroviruses and explain how they can be converted into state-of-the-art gene delivery tools with improved safety features. We discuss development of alpharetroviral vectors in compliance with regulatory requirements for clinical translation, and provide an outlook on possible future gene therapy applications. Taken together, this review is a broad overview of alpharetroviral vectors spanning the bridge from their parental virus discovery to their potential applicability in clinical settings.
[Mh] Termos MeSH primário: Alpharetrovirus/fisiologia
Terapia Genética/instrumentação
Neoplasias/terapia
[Mh] Termos MeSH secundário: Alpharetrovirus/genética
Animais
Terapia Genética/métodos
Vetores Genéticos/genética
Vetores Genéticos/fisiologia
Seres Humanos
Neoplasias/genética
Integração Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Em] Mês de entrada:1507
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141210
[St] Status:MEDLINE
[do] DOI:10.3390/v6124811



página 1 de 103 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde