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  1 / 123 MEDLINE  
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[PMID]:27902399
[Au] Autor:Walsh SR; de Jong JG; van Vloten JP; Gerpe MC; Santry LA; Wootton SK
[Ad] Endereço:Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
[Ti] Título:Truncation of the enzootic nasal tumor virus envelope protein cytoplasmic tail increases Env-mediated fusion and infectivity.
[So] Source:J Gen Virol;98(1):108-120, 2017 Jan.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Enzootic nasal tumor virus (ENTV) and Jaagsiekte sheep retrovirus (JSRV) are highly related ovine betaretroviruses that induce nasal and lung tumours in small ruminants, respectively. While the ENTV and JSRV envelope (Env) glycoproteins mediate virus entry using the same cellular receptor, the glycosylphosphatidylinositol-linked protein hyaluronoglucosaminidase, ENTV Env pseudovirions mediate entry into cells from a much more restricted range of species than do JSRV Env pseudovirions. Unlike JSRV Env, ENTV Env does not induce cell fusion at pH 5.0 or above, but rather requires a much lower pH (4.0-4.5) for fusion to occur. The cytoplasmic tail of retroviral envelope proteins is a key modulator of envelope-mediated fusion and pseudotype efficiency, especially in the context of virions composed of heterologous Gag proteins. Here we report that progressive truncation of the ENTV Env cytoplasmic tail improves transduction efficiency of pseudotyped retroviral vectors and that complete truncation of the ENTV Env cytoplasmic tail increases transduction efficiency to wild-type JSRV Env levels by increasing fusogenicity without affecting sensitivity to inhibition by lysosomotropic agents, subcellular localization or efficiency of inclusion into virions. Truncation of the cytoplasmic domain of ENTV Env resulted in a significant advantage in viral entry into all cell types tested, including foetal ovine lung and nasal cells. Taken together, we demonstrate that the cytoplasmic tail modulates the fusion activity of the ENTV Env protein and that truncation of this region enhances Eenv-mediated entry into target cells.
[Mh] Termos MeSH primário: Betaretrovirus/genética
Betaretrovirus/fisiologia
Deleção de Sequência
Proteínas do Envelope Viral/genética
Internalização do Vírus
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Seres Humanos
Transdução Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Envelope Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170501
[Lr] Data última revisão:
170501
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000654


  2 / 123 MEDLINE  
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[PMID]:28038674
[Au] Autor:Walsh SR; Gerpe MC; Wootton SK
[Ad] Endereço:Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
[Ti] Título:Construction of a molecular clone of ovine enzootic nasal tumor virus.
[So] Source:Virol J;13(1):209, 2016 Dec 30.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Enzootic nasal tumor virus (ENTV-1) is an ovine betaretrovirus that has been linked to enzootic nasal adenocarcinoma (ENA), a contagious tumor of the ethmoid turbinates of sheep. Transmission experiments performed using virus isolated from cell free nasal tumor homogenates suggest that ENTV-1 is the causative agent of ENA; however, this etiological relationship has not been conclusively proven due to the fact that the virus cannot be propagated in vitro nor is there an infectious molecular clone of the virus. METHODS: Here we report construction of a molecular clone of ENTV-1 and demonstrate that transfection of this molecular clone into HEK 293T cells produces mature virus particles. RESULTS: Analysis of recombinant virus particles derived from the initial molecular clone revealed a defect in the proteolytic processing of Gag; however, this defect could be corrected by co-expression of the Gag-Pro-Pol polyprotein from the highly related Jaagsiekte sheep retrovirus (JSRV) suggesting that the polyprotein cleavage sites in the ENTV-1 molecular clone were functional. Mutagenesis of the molecular clone to correct amino acid variants identified within the pro gene did not restore proteolytic processing; whereas deletion of one proline residue from a polyproline tract located in variable region 1 (VR1) of the matrix resulted in production of CA protein of the mature (cleaved) size strongly suggesting that normal virion morphogenesis and polyprotein cleavage took place. Finally, electron microscopy revealed the presence of spherical virus particles with an eccentric capsid and an average diameter of about 100 nm. CONCLUSION: In summary, we have constructed the first molecular clone of ENTV-1 from which mature virus particles can be produced. Future experiments using virus produced from this molecular clone can now be conducted to fulfill Koch's postulates and demonstrate that ENTV-1 is necessary and sufficient to induce ENA in sheep.
[Mh] Termos MeSH primário: Betaretrovirus/crescimento & desenvolvimento
Betaretrovirus/genética
Clonagem Molecular
Vírus Oncogênicos/crescimento & desenvolvimento
Vírus Oncogênicos/genética
[Mh] Termos MeSH secundário: Animais
Betaretrovirus/isolamento & purificação
Betaretrovirus/ultraestrutura
Linhagem Celular
Análise Mutacional de DNA
Células Epiteliais/virologia
Seres Humanos
Microscopia Eletrônica de Transmissão
Vírus Oncogênicos/isolamento & purificação
Vírus Oncogênicos/ultraestrutura
Poliproteínas/genética
Poliproteínas/metabolismo
Processamento de Proteína Pós-Traducional
Genética Reversa
Ovinos
Transfecção
Proteínas Virais/genética
Proteínas Virais/metabolismo
Vírion/ultraestrutura
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polyproteins); 0 (Viral Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170101
[St] Status:MEDLINE
[do] DOI:10.1186/s12985-016-0660-x


  3 / 123 MEDLINE  
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[PMID]:27609573
[Au] Autor:DU F; Chen D; Zhang Y; Sun X; Guo W; Liu S
[Ad] Endereço:College of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot 010018, China.
[Ti] Título:[Envelope protein of Jaagsiekte sheep retrovious expressed in NIH3T3 cells promotes cell proliferation].
[So] Source:Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi;32(9):1188-92, 2016 Sep.
[Is] ISSN:1007-8738
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:Objective To explore the influence of the exogenous Jaagsiekte sheep retrovious (exJSRV) envelope protein (Env) on NIH3T3 cell proliferation. Methods A recombinant plasmid pcDNA4/myc-His/exJSRV- env carrying exJSRV- env gene was constructed, and then the correctness of the recombinant plasmid was identified by PCR, restriction enzyme digestion and sequencing. The recombinant plasmid pcDNA4/myc-His/exJSRV- env was transiently transfected into NIH3T3 cells by Lipofectamine(TM) LTX. After the transfection of the recombinant plasmid, the expression of exJSRV- env was detected by reverse transcription PCR and Western blotting. The effect of Env on cell proliferation was investigated by CCK-8 assay and plate colony formation assay. Results The recombinant eukaryotic expression plasmid containing exJSRV- env was successfully constructed as identified by PCR, restriction enzyme identification and sequencing. After the recombinant plasmid was transiently transfected into NIH3T3 cells, reverse transcription PCR and Western blotting showed the expression of exJSRV- env , and Env promoted NIH3T3 cell proliferation significantly. Conclusion JSRV Env was expressed successfully in the NIH3T3 cells and promoted the proliferation of NIH3T3 cells.
[Mh] Termos MeSH primário: Betaretrovirus/genética
Proliferação Celular
Adenomatose Pulmonar Ovina/fisiopatologia
Adenomatose Pulmonar Ovina/virologia
Proteínas do Envelope Viral/genética
[Mh] Termos MeSH secundário: Animais
Betaretrovirus/metabolismo
Expressão Gênica
Camundongos
Células NIH 3T3
Ovinos
Transfecção
Proteínas do Envelope Viral/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Envelope Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170627
[Lr] Data última revisão:
170627
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160910
[St] Status:MEDLINE


  4 / 123 MEDLINE  
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[PMID]:27384664
[Au] Autor:Malicorne S; Vernochet C; Cornelis G; Mulot B; Delsuc F; Heidmann O; Heidmann T; Dupressoir A
[Ad] Endereço:Physiologie et Pathologie Moléculaires des Rétrovirus Endogènes et Infectieux, Unité Mixte de Recherche 9196, Centre National de la Recherche Scientifique, Institut Gustave Roussy, Villejuif, and Université Paris-Sud, Orsay, France.
[Ti] Título:Genome-Wide Screening of Retroviral Envelope Genes in the Nine-Banded Armadillo (Dasypus novemcinctus, Xenarthra) Reveals an Unfixed Chimeric Endogenous Betaretrovirus Using the ASCT2 Receptor.
[So] Source:J Virol;90(18):8132-49, 2016 Sep 15.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: Retroviruses enter host cells through the interaction of their envelope (Env) protein with a cell surface receptor, which triggers the fusion of viral and cellular membranes. The sodium-dependent neutral amino acid transporter ASCT2 is the common receptor of the large RD114 retrovirus interference group, whose members display frequent env recombination events. Germ line retrovirus infections have led to numerous inherited endogenous retroviruses (ERVs) in vertebrate genomes, which provide useful insights into the coevolutionary history of retroviruses and their hosts. Rare ERV-derived genes display conserved viral functions, as illustrated by the fusogenic syncytin env genes involved in placentation. Here, we searched for functional env genes in the nine-banded armadillo (Dasypus novemcinctus) genome and identified dasy-env1.1, which clusters with RD114 interference group env genes and with two syncytin genes sharing ASCT2 receptor usage. Using ex vivo pseudotyping and cell-cell fusion assays, we demonstrated that the Dasy-Env1.1 protein is fusogenic and can use both human and armadillo ASCT2s as receptors. This gammaretroviral env gene belongs to a provirus with betaretrovirus-like features, suggesting acquisition through recombination. Provirus insertion was found in several Dasypus species, where it has not reached fixation, whereas related family members integrated before diversification of the genus Dasypus >12 million years ago (Mya). This newly described ERV lineage is potentially useful as a population genetic marker. Our results extend the usage of ASCT2 as a retrovirus receptor to the mammalian clade Xenarthra and suggest that the acquisition of an ASCT2-interacting env gene is a major selective force driving the emergence of numerous chimeric viruses in vertebrates. IMPORTANCE: Retroviral infection is initiated by the binding of the viral envelope glycoprotein to a host cell receptor(s), triggering membrane fusion. Ancient germ line infections have generated numerous endogenous retroviruses (ERVs) in nearly all vertebrate genomes. Here, we report a previously uncharacterized ERV lineage from the genome of a xenarthran species, the nine-banded armadillo (Dasypus novemcinctus). It entered the Dasypus genus >12 Mya, with one element being inserted more recently in some Dasypus species, where it could serve as a useful marker for population genetics. This element exhibits an env gene, acquired by recombination events, with conserved viral fusogenic properties through binding to ASCT2, a receptor used by a wide range of recombinant retroviruses infecting other vertebrate orders. This specifies the ASCT2 transporter as a successful receptor for ERV endogenization and suggests that ASCT2-binding env acquisition events have favored the emergence of numerous chimeric viruses in a wide range of species.
[Mh] Termos MeSH primário: Sistema ASC de Transporte de Aminoácidos/metabolismo
Tatus/virologia
Betaretrovirus/isolamento & purificação
Retrovirus Endógenos/isolamento & purificação
Antígenos de Histocompatibilidade Menor/metabolismo
Provírus/isolamento & purificação
Receptores Virais/metabolismo
Proteínas do Envelope Viral/metabolismo
[Mh] Termos MeSH secundário: Animais
Betaretrovirus/genética
Retrovirus Endógenos/genética
Testes Genéticos
Provírus/genética
Recombinação Genética
Proteínas do Envelope Viral/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acid Transport System ASC); 0 (Minor Histocompatibility Antigens); 0 (Receptors, Virus); 0 (SLC1A5 protein, human); 0 (Viral Envelope Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170508
[Lr] Data última revisão:
170508
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160708
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.00483-16


  5 / 123 MEDLINE  
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[PMID]:26932456
[Au] Autor:Yee JL; Vanderford TH; Didier ES; Gray S; Lewis A; Roberts J; Taylor K; Bohm RP
[Ad] Endereço:California National Primate Research Center, University of California, Davis, CA, USA.
[Ti] Título:Specific pathogen free macaque colonies: a review of principles and recent advances for viral testing and colony management.
[So] Source:J Med Primatol;45(2):55-78, 2016 Apr.
[Is] ISSN:1600-0684
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:Specific pathogen free (SPF) macaques provide valuable animal models for biomedical research. In 1989, the National Center for Research Resources [now Office of Research Infrastructure Programs (ORIP)] of the National Institutes of Health initiated experimental research contracts to establish and maintain SPF colonies. The derivation and maintenance of SPF macaque colonies is a complex undertaking requiring knowledge of the biology of the agents for exclusion and normal physiology and behavior of macaques, application of the latest diagnostic technology, facilitiy management, and animal husbandry. This review provides information on the biology of the four viral agents targeted for exclusion in ORIP SPF macaque colonies, describes current state-of-the-art viral diagnostic algorithms, presents data from proficiency testing of diagnostic assays between laboratories at institutions participating in the ORIP SPF program, and outlines management strategies for maintaining the integrity of SPF colonies using results of diagnostic testing as a guide to decision making.
[Mh] Termos MeSH primário: Macaca
Doenças dos Macacos/diagnóstico
Viroses/veterinária
[Mh] Termos MeSH secundário: Algoritmos
Animais
Betaretrovirus/isolamento & purificação
Infecções por Deltaretrovirus/diagnóstico
Infecções por Deltaretrovirus/veterinária
Infecções por Herpesviridae/diagnóstico
Infecções por Herpesviridae/veterinária
Herpesvirus Cercopitecino 1/isolamento & purificação
Modelos Animais
Doenças dos Macacos/virologia
Controle de Qualidade
Infecções por Retroviridae/diagnóstico
Infecções por Retroviridae/veterinária
Síndrome de Imunodeficiência Adquirida dos Símios/diagnóstico
Vírus da Imunodeficiência Símia/isolamento & purificação
Vírus 1 Linfotrópico T de Símios/isolamento & purificação
Organismos Livres de Patógenos Específicos
Viroses/diagnóstico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; REVIEW
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170403
[Lr] Data última revisão:
170403
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160303
[St] Status:MEDLINE
[do] DOI:10.1111/jmp.12209


  6 / 123 MEDLINE  
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[PMID]:26755881
[Au] Autor:Lytvyak E; Montano-Loza AJ; Mason AL
[Ad] Endereço:Ellina Lytvyak, Aldo J Montano-Loza, Andrew L Mason, Division of Gastroenterology and Hepatology, University of Alberta, Edmonton T6G 2E1, Alberta, Canada.
[Ti] Título:Combination antiretroviral studies for patients with primary biliary cirrhosis.
[So] Source:World J Gastroenterol;22(1):349-60, 2016 Jan 07.
[Is] ISSN:2219-2840
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Following the characterization of a human betaretrovirus in patients with primary biliary cirrhosis (PBC), pilot studies using antiretroviral therapy have been conducted as proof of principal to establish a link of virus with disease and with the eventual aim to find better adjunct therapies for patients unresponsive to ursodeoxycholic acid. In the first open label pilot study, the reverse transcriptase inhibitor lamivudine had little demonstrable biochemical or histological effect after 1 year. Whereas, lamivudine in combination with zidovudine was associated with a significant reduction in alkaline phosphatase as well as improvement in necroinflammatory score, cholangitis and ductopenia over a 12 mo period. A double blind, multi-center randomized controlled trial using lamivudine with zidovudine for 6 mo confirmed a significant reduction in alkaline phosphatase, ALT and AST in patients on antiviral therapy. However, none of the patients achieved the stringent endpoint criteria for normalization of alkaline phosphatase. Furthermore, some patients developed biochemical rebound consistent with drug resistance. A major fault of these studies has been the inability to measure the viral load in peripheral blood and therefore, provide a direct correlation between improvement of hepatic biochemistry and reduction in viral load. Nevertheless, viral mutants to lamivudine with zidovudine were later characterized in the NOD.c3c4 mouse model of PBC that has been used to test other antiretroviral regimens to betaretrovirus. The combination of tenofovir and emtricitabine reverse transcriptase inhibitors and the HIV protease inhibitor, lopinavir were found to abrogate cholangitis in the NOD.c3c4 mouse model and the same regimen normalized the liver tests in a PBC patient with HIV and human betaretrovirus infection. This combination antiretroviral therapy has now been used in a double blind randomized controlled crossover study for patients with PBC followed by an open label extension study. Only a third of the PBC patients were able to tolerate the lopinavir but those maintained on tenofovir, emtricitabine and lopinavir experienced sustained and clinically meaningful reduction in hepatic biochemistry. While we await the histological and virological evaluation, it is clear that better tolerated regimens of antiretroviral treatment will be required in future clinical trials.
[Mh] Termos MeSH primário: Antirretrovirais/administração & dosagem
Cirrose Hepática Biliar/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Betaretrovirus/isolamento & purificação
Betaretrovirus/patogenicidade
Ensaios Clínicos como Assunto
Quimioterapia Combinada
Emtricitabina/administração & dosagem
Seres Humanos
Lamivudina/administração & dosagem
Cirrose Hepática Biliar/virologia
Lopinavir/administração & dosagem
Vírus do Tumor Mamário do Camundongo/patogenicidade
Camundongos
Tenofovir/administração & dosagem
Zidovudina/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Anti-Retroviral Agents); 2494G1JF75 (Lopinavir); 2T8Q726O95 (Lamivudine); 4B9XT59T7S (Zidovudine); 99YXE507IL (Tenofovir); G70B4ETF4S (Emtricitabine)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160113
[St] Status:MEDLINE
[do] DOI:10.3748/wjg.v22.i1.349


  7 / 123 MEDLINE  
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[PMID]:26744306
[Au] Autor:Walsh SR; Stinson KJ; Wootton SK
[Ad] Endereço:Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada. scott.walsh22@gmail.com.
[Ti] Título:Seroconversion of sheep experimentally infected with enzootic nasal tumor virus.
[So] Source:BMC Res Notes;9:15, 2016 Jan 07.
[Is] ISSN:1756-0500
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Enzootic nasal tumor virus (ENTV-1) is an exogenous betaretrovirus of sheep that transforms epithelial cells lining the ethmoid turbinates leading to a disease called enzootic nasal adenocarcinoma (ENA). A unique feature of ENA is the apparent absence of a specific humoral immune response to the virus, despite the highly productive infection in nasal tumors. The sheep genome contains approximately 27 copies of endogenous ovine betaretroviral sequences (enJSRVs) and expression of enJSRVs in the ovine placenta and uterine endometrium throughout gestation is thought to induce immunological tolerance to exogenous ovine betaretroviruses, a factor that may influence the likelihood of exogenous ENTV infection and disease outcome. Nevertheless, we recently demonstrated the presence of neutralizing antibodies directed against the ENTV-1 envelope glycoprotein in sheep naturally exposed to ENTV-1. FINDINGS: Here, we employed an ENTV-1 envelope glycoprotein surface subunit specific ELISA and a virus neutralization assay to monitor serum antibody responses to ENTV-1 in a group of lambs experimentally infected with ENTV-1 virus containing filtered ENA tumor homogenate. Seroconversion and development of neutralizing antibodies was detected in one of six experimentally infected lambs. CONCLUSIONS: Our results demonstrate that sheep can respond immunologically and seroconvert following ENTV-1 infection suggesting that anti-viral immune responses may play a role in the development of ENA.
[Mh] Termos MeSH primário: Betaretrovirus/fisiologia
Neoplasias Nasais/virologia
Infecções por Retroviridae/sangue
Infecções por Retroviridae/virologia
Soroconversão
Ovinos/sangue
Ovinos/virologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/sangue
Ensaio de Imunoadsorção Enzimática
Testes de Neutralização
Neoplasias Nasais/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160110
[Lr] Data última revisão:
160110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160109
[St] Status:MEDLINE
[do] DOI:10.1186/s13104-015-1824-2


  8 / 123 MEDLINE  
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[PMID]:26517399
[Au] Autor:Kramer P; Lausch V; Volkwein A; Hanke K; Hohn O; Bannert N
[Ad] Endereço:Robert Koch Institute, Division for HIV and Other Retroviruses, Nordufer 20, 13353 Berlin, Germany.
[Ti] Título:The human endogenous retrovirus K(HML-2) has a broad envelope-mediated cellular tropism and is prone to inhibition at a post-entry, pre-integration step.
[So] Source:Virology;487:121-8, 2016 Jan.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The HERV-K(HML-2) family is the most recent addition to the collection of human endogenous retroviruses. It comprises proviruses that encode functional proteins that can assemble into replication defective particles carrying the envelope protein. Using a reconstituted HERV-K113 envelope sequence, we have analyzed its ability to mediate entry into a set of 33 cell lines from 10 species. Of these, 30 were permissive, demonstrating an amphotropism consistent with a broad expression of receptor protein(s). In an initial effort to identify a receptor for HERV-K(HML-2) we investigated whether transferrin receptor 1 and hyaluronidase 2, known cellular receptors of the closely related betaretroviruses mouse mammary tumor virus (MMTV) and Jaagsiekte sheep retrovirus (JSRV), could facilitate HERV-K(HML-2) entry. However, neither of these proteins could serve as a receptor for HERV-K(HML-2). Moreover, during attempts to further characterize the tropism of HERV-K(HML-2), we identified a cellular activity that inhibits infection at a post-entry, pre-integration step.
[Mh] Termos MeSH primário: Antígenos CD/metabolismo
Moléculas de Adesão Celular/metabolismo
Retrovirus Endógenos/metabolismo
Hialuronoglucosaminidase/metabolismo
Receptores da Transferrina/metabolismo
Proteínas do Envelope Viral/metabolismo
Tropismo Viral/fisiologia
[Mh] Termos MeSH secundário: Células 3T3
Animais
Betaretrovirus/metabolismo
Células COS
Gatos
Linhagem Celular Tumoral
Cercopithecus aethiops
Cães
Proteínas Ligadas por GPI/metabolismo
Células HEK293
Células HeLa
Seres Humanos
Retrovirus Jaagsiekte de Ovinos/metabolismo
Vírus do Tumor Mamário do Camundongo/metabolismo
Camundongos
Receptores Virais
Células Vero
Internalização do Vírus
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (CD71 antigen); 0 (Cell Adhesion Molecules); 0 (GPI-Linked Proteins); 0 (Receptors, Transferrin); 0 (Receptors, Virus); 0 (Viral Envelope Proteins); EC 3.2.1.25 (Hyal2 protein, human); EC 3.2.1.35 (Hyaluronoglucosaminidase)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:151215
[Lr] Data última revisão:
151215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151031
[St] Status:MEDLINE


  9 / 123 MEDLINE  
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[PMID]:25717107
[Au] Autor:Escalera-Zamudio M; Mendoza ML; Heeger F; Loza-Rubio E; Rojas-Anaya E; Méndez-Ojeda ML; Taboada B; Mazzoni CJ; Arias CF; Greenwood AD
[Ad] Endereço:Leibniz Institute for Zoo and Wildlife Research (IZW), Berlin, Germany escalera@izw-berlin.de greenwood@izw-berlin.de.
[Ti] Título:A novel endogenous betaretrovirus in the common vampire bat (Desmodus rotundus) suggests multiple independent infection and cross-species transmission events.
[So] Source:J Virol;89(9):5180-4, 2015 May.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Desmodus rotundus endogenous betaretrovirus (DrERV) is fixed in the vampire bat D. rotundus population and in other phyllostomid bats but is not present in all species from this family. DrERV is not phylogenetically related to Old World bat betaretroviruses but to betaretroviruses from rodents and New World primates, suggesting recent cross-species transmission. A recent integration age estimation of the provirus in some taxa indicates that an exogenous counterpart might have been in recent circulation.
[Mh] Termos MeSH primário: Betaretrovirus/classificação
Quirópteros/genética
Quirópteros/virologia
Retrovirus Endógenos/classificação
Filogenia
Infecções por Retroviridae/veterinária
[Mh] Termos MeSH secundário: Animais
Betaretrovirus/genética
Betaretrovirus/isolamento & purificação
Retrovirus Endógenos/genética
Retrovirus Endógenos/isolamento & purificação
Ordem dos Genes
Primatas/virologia
Infecções por Retroviridae/virologia
Roedores/virologia
Sintenia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1506
[Cu] Atualização por classe:151101
[Lr] Data última revisão:
151101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150227
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.03452-14


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[PMID]:25631652
[Au] Autor:Wang W; Indik S; Wong GK; Mason AL
[Ad] Endereço:Center of Excellence for Gastrointestinal Inflammation and Immunity Research, University of Alberta, Edmonton, AB, Canada.
[Ti] Título:Editorial: Betaretrovirus in biliary epithelia of patients with autoimmune and cryptogenic liver disease - authors' reply.
[So] Source:Aliment Pharmacol Ther;41(5):491, 2015 Mar.
[Is] ISSN:1365-2036
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Betaretrovirus
Hepatite Autoimune/virologia
Hepatócitos/virologia
Cirrose Hepática Biliar/virologia
[Mh] Termos MeSH secundário: Feminino
Seres Humanos
Masculino
[Pt] Tipo de publicação:COMMENT; EDITORIAL
[Em] Mês de entrada:1506
[Cu] Atualização por classe:151028
[Lr] Data última revisão:
151028
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150130
[St] Status:MEDLINE
[do] DOI:10.1111/apt.13082



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