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  1 / 134 MEDLINE  
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[PMID]:28301970
[Au] Autor:Bandeira VS; Tomás HA; Alici E; Carrondo MJ; Coroadinha AS
[Ad] Endereço:1 Instituto de Biologia Experimental e Tecnológica , Oeiras, Portugal .
[Ti] Título:Disclosing the Parameters Leading to High Productivity of Retroviral Producer Cells Lines: Evaluating Random Versus Targeted Integration.
[So] Source:Hum Gene Ther Methods;28(2):78-90, 2017 Apr.
[Is] ISSN:1946-6544
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gammaretrovirus and lentivirus are the preferred viral vectors to genetically modify T and natural killer cells to be used in immune cell therapies. The transduction efficiency of hematopoietic and T cells is more efficient using gibbon ape leukemia virus (GaLV) pseudotyping. In this context gammaretroviral vector producer cells offer competitive higher titers than transient lentiviral vectors productions. The main aim of this work was to identify the key parameters governing GaLV-pseudotyped gammaretroviral vector productivity in stable producer cells, using a retroviral vector expression cassette enabling positive (facilitating cell enrichment) and negative cell selection (allowing cell elimination). The retroviral vector contains a thymidine kinase suicide gene fused with a ouabain-resistant Na ,K -ATPase gene, a potential safer and faster marker. The establishment of retroviral vector producer cells is traditionally performed by randomly integrating the retroviral vector expression cassette codifying the transgene. More recently, recombinase-mediated cassette exchange methodologies have been introduced to achieve targeted integration. Herein we compared random and targeted integration of the retroviral vector transgene construct. Two retroviral producer cell lines, 293 OuaS and 293 FlexOuaS, were generated by random and targeted integration, respectively, producing high titers (on the order of 10 infectious particles·ml ). Results showed that the retroviral vector transgene cassette is the key retroviral vector component determining the viral titers notwithstanding, single-copy integration is sufficient to provide high titers. The expression levels of the three retroviral constructs (gag-pol, GaLV env, and retroviral vector transgene) were analyzed. Although gag-pol and GaLV env gene expression levels should surpass a minimal threshold, we found that relatively modest expression levels of these two expression cassettes are required. Their levels of expression should not be maximized. We concluded, to establish a high producer retroviral vector cell line only the expression level of the genomic retroviral RNA, that is, the retroviral vector transgene cassette, should be maximized, both through (1) the optimization of its design (i.e., genetic elements composition) and (2) the selection of high expressing chromosomal locus for its integration. The use of methodologies identifying and promoting integration into high-expression loci, as targeted integration or high-throughput screening are in this perspective highly valuable.
[Mh] Termos MeSH primário: Terapia Genética
Vetores Genéticos/genética
Retroviridae/genética
Integração Viral/genética
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Expressão Gênica
Genes Transgênicos Suicidas/genética
Vetores Genéticos/uso terapêutico
Seres Humanos
Lentivirus/genética
Vírus da Leucemia do Macaco Gibão/genética
ATPase Trocadora de Sódio-Potássio/genética
Timidina Quinase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 2.7.1.21 (Thymidine Kinase); EC 3.6.3.9 (Sodium-Potassium-Exchanging ATPase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170318
[St] Status:MEDLINE
[do] DOI:10.1089/hgtb.2016.149


  2 / 134 MEDLINE  
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[PMID]:28220345
[Au] Autor:McKee J; Clark N; Shapter F; Simmons G
[Ad] Endereço:Ecosure, Burleigh Heads, QLD, Australia.
[Ti] Título:A new look at the origins of gibbon ape leukemia virus.
[So] Source:Virus Genes;53(2):165-172, 2017 Apr.
[Is] ISSN:1572-994X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Is the origin of gibbon ape leukemia virus (GALV) human after all? When GALV was discovered and found to cause neoplastic disease in gibbons, it stimulated a great deal of research including investigations into the origins of this virus. A number of publications have suggested that the GALV progenitor was a retrovirus present in one of several species of South East Asian rodents that had close contact with captive gibbons. However, there are no published retroviral sequences from any South East Asian species to support this view. Here we present an alternative hypothesis that the origin of GALV is a virus closely related to Melomys burtoni retrovirus, and that this virus infected human patients in Papua New Guinea from whom biological material was obtained or in some way contaminated these samples. This material we propose contained infectious MbRV-related virus that was then unwittingly introduced into gibbons which subsequently developed GALV infections.
[Mh] Termos MeSH primário: Hylobates/virologia
Vírus da Leucemia do Macaco Gibão/genética
RNA Viral/genética
Infecções por Retroviridae/genética
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Hylobates/genética
Vírus da Leucemia do Macaco Gibão/patogenicidade
Filogenia
Retroviridae/genética
Retroviridae/patogenicidade
Infecções por Retroviridae/virologia
Roedores/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170222
[St] Status:MEDLINE
[do] DOI:10.1007/s11262-017-1436-0


  3 / 134 MEDLINE  
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[PMID]:27384662
[Au] Autor:Alfano N; Michaux J; Morand S; Aplin K; Tsangaras K; Löber U; Fabre PH; Fitriana Y; Semiadi G; Ishida Y; Helgen KM; Roca AL; Eiden MV; Greenwood AD
[Ad] Endereço:Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany.
[Ti] Título:Endogenous Gibbon Ape Leukemia Virus Identified in a Rodent (Melomys burtoni subsp.) from Wallacea (Indonesia).
[So] Source:J Virol;90(18):8169-80, 2016 Sep 15.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: Gibbon ape leukemia virus (GALV) and koala retrovirus (KoRV) most likely originated from a cross-species transmission of an ancestral retrovirus into koalas and gibbons via one or more intermediate as-yet-unknown hosts. A virus highly similar to GALV has been identified in an Australian native rodent (Melomys burtoni) after extensive screening of Australian wildlife. GALV-like viruses have also been discovered in several Southeast Asian species, although screening has not been extensive and viruses discovered to date are only distantly related to GALV. We therefore screened 26 Southeast Asian rodent species for KoRV- and GALV-like sequences, using hybridization capture and high-throughput sequencing, in the attempt to identify potential GALV and KoRV hosts. Only the individuals belonging to a newly discovered subspecies of Melomys burtoni from Indonesia were positive, yielding an endogenous provirus very closely related to a strain of GALV. The sequence of the critical receptor domain for GALV infection in the Indonesian M. burtoni subsp. was consistent with the susceptibility of the species to GALV infection. The second record of a GALV in M. burtoni provides further evidence that M. burtoni, and potentially other lineages within the widespread subfamily Murinae, may play a role in the spread of GALV-like viruses. The discovery of a GALV in the most western part of the Australo-Papuan distribution of M. burtoni, specifically in a transitional zone between Asia and Australia (Wallacea), may be relevant to the cross-species transmission to gibbons in Southeast Asia and broadens the known distribution of GALVs in wild rodents. IMPORTANCE: Gibbon ape leukemia virus (GALV) and the koala retrovirus (KoRV) are very closely related, yet their hosts neither are closely related nor overlap geographically. Direct cross-species infection between koalas and gibbons is unlikely. Therefore, GALV and KoRV may have arisen via a cross-species transfer from an intermediate host whose range overlaps those of both gibbons and koalas. Using hybridization capture and high-throughput sequencing, we have screened a wide range of rodent candidate hosts from Southeast Asia for KoRV- and GALV-like sequences. Only a Melomys burtoni subspecies from Wallacea (Indonesia) was positive for GALV. We report the genome sequence of this newly identified GALV, the critical domain for infection of its potential cellular receptor, and its phylogenetic relationships with the other previously characterized GALVs. We hypothesize that Melomys burtoni, and potentially related lineages with an Australo-Papuan distribution, may have played a key role in cross-species transmission to other taxa.
[Mh] Termos MeSH primário: Vírus da Leucemia do Macaco Gibão/isolamento & purificação
Murinae/virologia
Infecções por Retroviridae/veterinária
Doenças dos Roedores/virologia
[Mh] Termos MeSH secundário: Animais
Sequenciamento de Nucleotídeos em Larga Escala
Indonésia
Vírus da Leucemia do Macaco Gibão/genética
Hibridização de Ácido Nucleico
Provírus/genética
Provírus/isolamento & purificação
Infecções por Retroviridae/virologia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170508
[Lr] Data última revisão:
170508
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160708
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.00723-16


  4 / 134 MEDLINE  
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[PMID]:26668323
[Au] Autor:Majdoul S; Seye AK; Kichler A; Holic N; Galy A; Bechinger B; Fenard D
[Ad] Endereço:From Généthon, 91000 Evry, France, INSERM UMR_S951, 91000 Evry, France, University of Evry, 91000 Evry, France.
[Ti] Título:Molecular Determinants of Vectofusin-1 and Its Derivatives for the Enhancement of Lentivirally Mediated Gene Transfer into Hematopoietic Stem/Progenitor Cells.
[So] Source:J Biol Chem;291(5):2161-9, 2016 Jan 29.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gene delivery into hCD34+ hematopoietic stem/progenitor cells (HSPCs) using human immunodeficiency virus, type 1-derived lentiviral vectors (LVs) has several promising therapeutic applications. Numerous clinical trials are currently underway. However, the efficiency, safety, and cost of LV gene therapy could be ameliorated by enhancing target cell transduction levels and reducing the amount of LV used on the cells. Several transduction enhancers already exist, such as fibronectin fragments or cationic compounds. Recently, we discovered Vectofusin-1, a new transduction enhancer, also called LAH4-A4, a short histidine-rich amphipathic peptide derived from the LAH4 family of DNA transfection agents. Vectofusin-1 enhances the infectivity of lentiviral and γ-retroviral vectors pseudotyped with various envelope glycoproteins. In this study, we compared a family of Vectofusin-1 isomers and showed that Vectofusin-1 remains the lead peptide for HSPC transduction enhancement with LVs pseudotyped with vesicular stomatitis virus glycoproteins and also with modified gibbon ape leukemia virus glycoproteins. By comparing the capacity of numerous Vectofusin-1 variants to promote the modified gibbon ape leukemia virus glycoprotein-pseudotyped lentiviral vector infectivity of HSPCs, the lysine residues on the N-terminal extremity of Vectofusin-1, a hydrophilic angle of 140° formed by the histidine residues in the Schiffer-Edmundson helical wheel representation, hydrophobic residues consisting of leucine were all found to be essential and helped to define a minimal active sequence. The data also show that the critical determinants necessary for lentiviral transduction enhancement are partially different from those necessary for efficient antibiotic or DNA transfection activity of LAH4 derivatives. In conclusion, these results help to decipher the action mechanism of Vectofusin-1 in the context of hCD34+ cell-based gene therapy.
[Mh] Termos MeSH primário: Peptídeos Catiônicos Antimicrobianos/química
Técnicas de Transferência de Genes
Vetores Genéticos
Células-Tronco Hematopoéticas/citologia
Lentivirus
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Antígenos CD34/metabolismo
DNA/química
Terapia Genética/métodos
Glicoproteínas/química
Células HCT116
Células HEK293
HIV-1/metabolismo
Células HeLa
Histidina/química
Seres Humanos
Vírus da Leucemia do Macaco Gibão
Dados de Sequência Molecular
Peptídeos/química
Homologia de Sequência de Aminoácidos
Transdução Genética
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, CD34); 0 (Antimicrobial Cationic Peptides); 0 (Glycoproteins); 0 (LAH(4) protein, synthetic); 0 (Peptides); 4QD397987E (Histidine); 9007-49-2 (DNA)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151216
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M115.675033


  5 / 134 MEDLINE  
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[PMID]:26637454
[Au] Autor:Alfano N; Kolokotronis SO; Tsangaras K; Roca AL; Xu W; Eiden MV; Greenwood AD
[Ad] Endereço:Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany.
[Ti] Título:Episodic Diversifying Selection Shaped the Genomes of Gibbon Ape Leukemia Virus and Related Gammaretroviruses.
[So] Source:J Virol;90(4):1757-72, 2015 Dec 04.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: Gibbon ape leukemia viruses (GALVs) are part of a larger group of pathogenic gammaretroviruses present across phylogenetically diverse host species of Australasian mammals. Despite the biomedical utility of GALVs as viral vectors and in cancer gene therapy, full genome sequences have not been determined for all of the five identified GALV strains, nor has a comprehensive evolutionary analysis been performed. We therefore generated complete genomic sequences for each GALV strain using hybridization capture and high-throughput sequencing. The four strains of GALV isolated from gibbons formed a monophyletic clade that was closely related to the woolly monkey virus (WMV), which is a GALV strain that likely originated in a gibbon host. The GALV-WMV clade in turn formed a sister group to the koala retroviruses (KoRVs). Genomic signatures of episodic diversifying selection were detected among the gammaretroviruses with concentration in the env gene across the GALV strains that were particularly oncogenic and KoRV strains that were potentially exogenous, likely reflecting their adaptation to the host immune system. In vitro studies involving vectors chimeric between GALV and KoRV-B established that variable regions A and B of the surface unit of the envelope determine which receptor is used by a viral strain to enter host cells. IMPORTANCE: The gibbon ape leukemia viruses (GALVs) are among the most medically relevant retroviruses due to their use as viral vectors for gene transfer and in cancer gene therapy. Despite their importance, full genome sequences have not been determined for the majority of primate isolates, nor has comprehensive evolutionary analysis been performed, despite evidence that the viruses are facing complex selective pressures associated with cross-species transmission. Using hybridization capture and high-throughput sequencing, we report here the full genome sequences of all the GALV strains and demonstrate that diversifying selection is acting on them, particularly in the envelope gene in functionally important domains, suggesting that host immune pressure is shaping GALV evolution.
[Mh] Termos MeSH primário: Evolução Molecular
Hylobates/virologia
Vírus da Leucemia do Macaco Gibão/genética
Seleção Genética
[Mh] Termos MeSH secundário: Animais
Australásia
Análise por Conglomerados
Produtos do Gene env/genética
Vetores Genéticos
Genoma Viral
Sequenciamento de Nucleotídeos em Larga Escala
Dados de Sequência Molecular
Phascolarctidae
Filogenia
RNA Viral/genética
Recombinação Genética
Análise de Sequência de DNA
Homologia de Sequência
Internalização do Vírus
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, N.I.H., INTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Gene Products, env); 0 (RNA, Viral)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151206
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.02745-15


  6 / 134 MEDLINE  
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[PMID]:25831573
[Au] Autor:Siegal-Willott JL; Jensen N; Kimi D; Taliaferro D; Blankenship T; Malinsky B; Murray S; Eiden MV; Xu W
[Ti] Título:Evaluation of captive gibbons (Hylobates spp., Nomascus spp., Symphalangus spp.) in North American Zoological Institutions for Gibbon Ape Leukemia Virus (GALV).
[So] Source:J Zoo Wildl Med;46(1):27-33, 2015 Mar.
[Is] ISSN:1042-7260
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study evaluated 79 captive gibbons (Hylobates, Nomascus, and Symphalangus spp.) within 30 North American zoological institutions for evidence of exposure to and possible infection with gibbon ape leukemia virus (GALV). Enzyme-linked immunosorbent assays (ELISAs) on gibbon serum samples revealed the presence of antibodies against GALV antigens in 28% of animals, indicating previous exposure or possibly protective immunity to GALV. Virus detection in gibbon blood or serum using polymerase chain reaction (PCR) or co-culture of gibbon peripheral blood mononuclear cells with human cells was negative for all samples submitted. The majority (19/27, 70%) of animals with reported health conditions were clinically healthy at the time of sample collection. Historically accrued clinical data were used to assess association of diseases in gibbons antibody positive for GALV. The results suggest captive gibbons could mount an immune response to GALV and show no evidence of infection. There was no association with neoplastic conditions in seropositive animals. The potential role of gibbons as a reservoir for GALV and the role of GALV as an epizoonotic-zoonotic agent or as a contributor to gibbon ape morbidity and mortality are not substantiated by the study findings.
[Mh] Termos MeSH primário: Doenças dos Símios Antropoides/virologia
Hylobates/sangue
Vírus da Leucemia do Macaco Gibão/isolamento & purificação
Leucemia/veterinária
Infecções por Retroviridae/veterinária
Infecções Tumorais por Vírus/veterinária
[Mh] Termos MeSH secundário: Animais
Animais de Zoológico
Anticorpos Antivirais/sangue
Doenças dos Símios Antropoides/epidemiologia
Linhagem Celular
Ensaio de Imunoadsorção Enzimática/métodos
Ensaio de Imunoadsorção Enzimática/veterinária
Seres Humanos
Leucemia/epidemiologia
Leucemia/virologia
América do Norte/epidemiologia
Infecções por Retroviridae/epidemiologia
Infecções por Retroviridae/virologia
Especificidade da Espécie
Infecções Tumorais por Vírus/epidemiologia
Infecções Tumorais por Vírus/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., INTRAMURAL
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:150813
[Lr] Data última revisão:
150813
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150403
[St] Status:MEDLINE


  7 / 134 MEDLINE  
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[PMID]:25751502
[Au] Autor:Wang X; Olszewska M; Qu J; Wasielewska T; Bartido S; Hermetet G; Sadelain M; Rivière I
[Ad] Endereço:*Cell Therapy and Cell Engineering Facility †Molecular Pharmacology and Chemistry Program §Center for Cell Engineering, Memorial Sloan Kettering Cancer Center, New York ‡Pall Life Sciences, Port Washington, NY.
[Ti] Título:Large-scale clinical-grade retroviral vector production in a fixed-bed bioreactor.
[So] Source:J Immunother;38(3):127-35, 2015 Apr.
[Is] ISSN:1537-4513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The successful genetic engineering of patient T cells with γ-retroviral vectors expressing chimeric antigen receptors or T-cell receptors for phase II clinical trials and beyond requires the large-scale manufacture of high-titer vector stocks. The production of retroviral vectors from stable packaging cell lines using roller bottles or 10- to 40-layer cell factories is limited by a narrow harvest window, labor intensity, open-system operations, and the requirement for significant incubator space. To circumvent these shortcomings, we optimized the production of vector stocks in a disposable fixed-bed bioreactor using good manufacturing practice-grade packaging cell lines. High-titer vector stocks were harvested over 10 days, representing a much broader harvest window than the 3-day harvest afforded by cell factories. For PG13 and 293Vec packaging cells, the average vector titer and the vector stocks' yield in the bioreactor were higher by 3.2- to 7.3-fold, and 5.6- to 13.1-fold, respectively, than those obtained in cell factories. The vector production was 10.4 and 18.6 times more efficient than in cell factories for PG13 and 293Vec cells, respectively. Furthermore, the vectors produced from the fixed-bed bioreactors passed the release test assays for clinical applications. Therefore, a single vector lot derived from 293Vec is suitable to transduce up to 500 patients cell doses in the context of large clinical trials using chimeric antigen receptors or T-cell receptors. These findings demonstrate for the first time that a robust fixed-bed bioreactor process can be used to produce γ-retroviral vector stocks scalable up to the commercialization phase.
[Mh] Termos MeSH primário: Técnicas de Cultura Celular por Lotes/métodos
Técnicas de Cultura Celular por Lotes/normas
Reatores Biológicos
Vetores Genéticos/genética
Vetores Genéticos/normas
Retroviridae/genética
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Transformada
Células HEK293
Seres Humanos
Vírus da Leucemia do Macaco Gibão/genética
Linfócitos T/metabolismo
Transdução Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Em] Mês de entrada:1511
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150310
[St] Status:MEDLINE
[do] DOI:10.1097/CJI.0000000000000072


  8 / 134 MEDLINE  
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[PMID]:25251014
[Au] Autor:Simmons G; Clarke D; McKee J; Young P; Meers J
[Ad] Endereço:School of Veterinary Science, The University of Queensland, Gatton, Queensland, Australia.
[Ti] Título:Discovery of a novel retrovirus sequence in an Australian native rodent (Melomys burtoni): a putative link between gibbon ape leukemia virus and koala retrovirus.
[So] Source:PLoS One;9(9):e106954, 2014.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gibbon ape leukaemia virus (GALV) and koala retrovirus (KoRV) share a remarkably close sequence identity despite the fact that they occur in distantly related mammals on different continents. It has previously been suggested that infection of their respective hosts may have occurred as a result of a species jump from another, as yet unidentified vertebrate host. To investigate possible sources of these retroviruses in the Australian context, DNA samples were obtained from 42 vertebrate species and screened using PCR in order to detect proviral sequences closely related to KoRV and GALV. Four proviral partial sequences totalling 2880 bases which share a strong similarity with KoRV and GALV were detected in DNA from a native Australian rodent, the grassland melomys, Melomys burtoni. We have designated this novel gammaretrovirus Melomys burtoni retrovirus (MbRV). The concatenated nucleotide sequence of MbRV shares 93% identity with the corresponding sequence from GALV-SEATO and 83% identity with KoRV. The geographic ranges of the grassland melomys and of the koala partially overlap. Thus a species jump by MbRV from melomys to koalas is conceivable. However the genus Melomys does not occur in mainland South East Asia and so it appears most likely that another as yet unidentified host was the source of GALV.
[Mh] Termos MeSH primário: Produtos do Gene pol/genética
Murinae/virologia
Retroviridae/genética
[Mh] Termos MeSH secundário: Animais
Austrália
Células Cultivadas
Cercopithecus aethiops
DNA Viral/química
DNA Viral/genética
Regulação Viral da Expressão Gênica
Produtos do Gene env/genética
Hylobates/virologia
Vírus da Leucemia do Macaco Gibão/genética
Masculino
Dados de Sequência Molecular
Phascolarctidae/virologia
Filogenia
Reação em Cadeia da Polimerase
RNA Viral/genética
Retroviridae/classificação
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Análise de Sequência de DNA
Células Vero
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Gene Products, env); 0 (Gene Products, pol); 0 (RNA, Viral)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:151029
[Lr] Data última revisão:
151029
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140925
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0106954


  9 / 134 MEDLINE  
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[PMID]:24694535
[Au] Autor:Koste L; Beissert T; Hoff H; Pretsch L; Türeci Ö; Sahin U
[Ad] Endereço:III Medical Clinic at the University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany.
[Ti] Título:T-cell receptor transfer into human T cells with ecotropic retroviral vectors.
[So] Source:Gene Ther;21(5):533-8, 2014 May.
[Is] ISSN:1476-5462
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Adoptive T-cell transfer for cancer immunotherapy requires genetic modification of T cells with recombinant T-cell receptors (TCRs). Amphotropic retroviral vectors (RVs) used for TCR transduction for this purpose are considered safe in principle. Despite this, TCR-coding and packaging vectors could theoretically recombine to produce replication competent vectors (RCVs), and transduced T-cell preparations must be proven free of RCV. To eliminate the need for RCV testing, we transduced human T cells with ecotropic RVs so potential RCV would be non-infectious for human cells. We show that transfection of synthetic messenger RNA encoding murine cationic amino-acid transporter 1 (mCAT-1), the receptor for murine retroviruses, enables efficient transient ecotropic transduction of human T cells. mCAT-1-dependent transduction was more efficient than amphotropic transduction performed in parallel, and preferentially targeted naive T cells. Moreover, we demonstrate that ecotropic TCR transduction results in antigen-specific restimulation of primary human T cells. Thus, ecotropic RVs represent a versatile, safe and potent tool to prepare T cells for the adoptive transfer.
[Mh] Termos MeSH primário: Linfócitos T CD4-Positivos/citologia
Linfócitos T CD8-Positivos/citologia
Transportador 1 de Aminoácidos Catiônicos/genética
Receptores de Antígenos de Linfócitos T/genética
Retroviridae/genética
[Mh] Termos MeSH secundário: Transferência Adotiva
Animais
Linfócitos T CD4-Positivos/transplante
Linfócitos T CD8-Positivos/transplante
Linhagem Celular
Eletroporação
Vetores Genéticos
Células HEK293
Seres Humanos
Imunoterapia Adotiva
Células Jurkat
Vírus da Leucemia do Macaco Gibão/genética
Glicoproteínas de Membrana/genética
Camundongos
Plasmídeos/genética
RNA Mensageiro/genética
Transdução Genética
Vírus da Estomatite Vesicular Indiana/genética
Proteínas do Envelope Viral/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cationic Amino Acid Transporter 1); 0 (G protein, vesicular stomatitis virus); 0 (Membrane Glycoproteins); 0 (RNA, Messenger); 0 (Receptors, Antigen, T-Cell); 0 (Viral Envelope Proteins)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:140508
[Lr] Data última revisão:
140508
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140404
[St] Status:MEDLINE
[do] DOI:10.1038/gt.2014.25


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[PMID]:24687598
[Au] Autor:Bing Z; Jianru Y; Yuequan J; Shifeng C; Xinping F
[Ad] Endereço:Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, China, drzhubing@tom.com.
[Ti] Título:Packaging, amplification, and appraisal of the recombinant tumor-selective type I herpes simplex virus carrying GALV.fus gene.
[So] Source:Cell Biochem Biophys;70(1):321-6, 2014 Sep.
[Is] ISSN:1559-0283
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of the study was to successfully construct three plasmids, which include the GALV.fus gene plasmid regulated by the herpes simplex virus type 1 (HSV-1) late expression gene-UL38 promoter and induced by HSV-1 (HSV-UL38P-GALV.fus), the cytomegalovirus promoter without tumor specificity (CMVP) GALV.fus plasmid (HSV-CMVP-GALV.fus), and the control plasmid in which the GALV.fus gene fragment was replaced by the enhanced green fluorescent protein (EGFP) gene fragment (HSV-CMVP-EGFP). The three constructed plasmids were all packaged and named as Synco-2, Synco-1, and Baco-1. The plasmids were amplified in coliform bacterium and transfected into Vero cells using lipofectamine. These recombinant HSV-1 were amplified in Vero cells and purified by conventional methods of cesium chloride, TCID50 method is used to measure virus titers. The total RNA was then extracted from the HepG2 cells transfected by Synco-1 and Synco-2, and the expression of GALV.fus mRNA was detected by RT-PCR. The three recombinant HSV-1 vectors were propagated in Vero cells and purified by cesium chloride density gradient centrifugation, titrated by TCID50 method, and packaged. The titers of Baco-1, Synco-1, and Synco-2 were 3 × 10(10), 1 × 10(11), and 4 × 10(10) pfu/ml. The GALV.fus gene was identified in the infected HepG2 cells by RT-PCR method.
[Mh] Termos MeSH primário: DNA Recombinante/genética
Engenharia Genética/métodos
Herpesvirus Humano 1/genética
Vírus da Leucemia do Macaco Gibão/genética
Técnicas de Amplificação de Ácido Nucleico
Proteína FUS de Ligação a RNA/genética
Montagem de Vírus
[Mh] Termos MeSH secundário: Animais
Cercopithecus aethiops
Terapia Genética
Células Hep G2
Herpesvirus Humano 1/fisiologia
Seres Humanos
Neoplasias Pulmonares/genética
Neoplasias Pulmonares/terapia
Plasmídeos/genética
RNA/genética
RNA/isolamento & purificação
Transfecção
Células Vero
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Recombinant); 0 (RNA-Binding Protein FUS); 63231-63-0 (RNA)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:140814
[Lr] Data última revisão:
140814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140402
[St] Status:MEDLINE
[do] DOI:10.1007/s12013-014-9915-6



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