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  1 / 211 MEDLINE  
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[PMID]:27609630
[Au] Autor:Zao CL; Tomanek L; Cooke A; Berger R; Yang L; Xie C; Chen S; Shi C; Rong R
[Ad] Endereço:1​VRL-San Antonio, San Antonio, TX, USA.
[Ti] Título:A novel simian retrovirus subtype discovered in cynomolgus monkeys (Macaca fascicularis).
[So] Source:J Gen Virol;97(11):3017-3023, 2016 Nov.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A new simian retrovirus (SRV) subtype was discovered in China and the USA from Cambodian-origin cynomolgus monkeys. Histopathological examination from necropsied animals showed multifocal lymphoplasmacystic and histocytic inflammation. The complete genome sequences demonstrated that the US virus isolates were nearly identical (99.91-99.93 %) and differed only slightly (99.13-99.16 % identical) from the China isolate. Phylogenetic analysis showed that the new virus isolates formed a distinct branch of SRV-1 through -7, and therefore were named this subtype, SRV-8. This SRV-8 variant was also phylogenetically and serologically more closely related to SRV-4 than any other SRV subtype.
[Mh] Termos MeSH primário: Doenças dos Macacos/virologia
Infecções por Retroviridae/veterinária
Retrovirus dos Símios/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Macaca fascicularis/virologia
Fases de Leitura Aberta
Filogenia
Infecções por Retroviridae/virologia
Retrovirus dos Símios/classificação
Retrovirus dos Símios/genética
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170503
[Lr] Data última revisão:
170503
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160910
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000601


  2 / 211 MEDLINE  
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[PMID]:27275923
[Au] Autor:Srinivasan P; Moss JA; Gunawardana M; Churchman SA; Yang F; Dinh CT; Mitchell JM; Zhang J; Fanter R; Miller CS; Butkyavichene I; McNicholl JM; Smith TJ; Baum MM; Smith JM
[Ad] Endereço:Laboratory Branch, Division of HIV/AIDS Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.
[Ti] Título:Topical Delivery of Tenofovir Disoproxil Fumarate and Emtricitabine from Pod-Intravaginal Rings Protects Macaques from Multiple SHIV Exposures.
[So] Source:PLoS One;11(6):e0157061, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Topical preexposure prophylaxis (PrEP) against HIV has been marginally successful in recent clinical trials with low adherence rates being a primary factor for failure. Controlled, sustained release of antiretroviral (ARV) drugs may help overcome these low adherence rates if the product is protective for extended periods of time. The oral combination of tenofovir disoproxil fumarate (TDF) and emtricitabine (FTC) is currently the only FDA-approved ARV drug for HIV PrEP. A novel pod-intravaginal ring (IVR) delivering TDF and FTC at independently controlled rates was evaluated for efficacy at preventing SHIV162p3 infection in a rigorous, repeat low-dose vaginal exposure model using normally cycling female pigtailed macaques. Six macaques received pod-IVRs containing TDF (65 mg) and FTC (68 mg) every two weeks, and weekly vaginal exposures to 50 TCID50 of SHIV162p3 began one week after the first pod-IVR insertion. All pod-IVR-treated macaques were fully protected throughout the study (P = 0.0002, Log-rank test), whereas all control animals became infected with a median of 4 exposures to infection. The topical, sustained release of TDF and FTC from the pod-IVR maintained protective drug levels in macaques over four months of virus exposures. This novel and versatile delivery system has the capacity to deliver and maintain protective levels of multiple drugs and the protection observed here warrants clinical evaluation of this pod-IVR design.
[Mh] Termos MeSH primário: Dispositivos Anticoncepcionais Femininos
Emtricitabina/farmacologia
Retrovirus dos Símios
Síndrome de Imunodeficiência Adquirida dos Símios/prevenção & controle
Tenofovir/farmacologia
[Mh] Termos MeSH secundário: Administração Intravaginal
Administração Tópica
Animais
Feminino
Macaca nemestrina
Síndrome de Imunodeficiência Adquirida dos Símios/transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
99YXE507IL (Tenofovir); G70B4ETF4S (Emtricitabine)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160609
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0157061


  3 / 211 MEDLINE  
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[PMID]:27113161
[Au] Autor:Fukumoto H; Hishima T; Hasegawa H; Saeki H; Kuroda M; Katano H
[Ad] Endereço:Department of Pathology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan; Department of Dermatology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8603, Japan.
[Ti] Título:Evaluation of Vero-cell-derived simian endogenous retrovirus infection in humans by detection of viral genome in clinicopathological samples and commercialized vaccines and by serology of Japanese general population.
[So] Source:Vaccine;34(24):2700-6, 2016 May 23.
[Is] ISSN:1873-2518
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Vero cells are used in laboratories for the isolation of viruses and the production of vaccines. Recently, the sequence of simian endogenous retrovirus (SERV) was identified in Vero cells (SERVagm-Vero), with homology to exogenously transmitted, pathogenic simian retroviruses (SRVs). Although SERVagm-Vero was shown to be noninfectious to human cells in vitro, SERV infection in humans is controversial. In this study, we evaluated the status of SERV infection in humans by detecting the viral genome in clinicopathological samples and commercialized vaccines, and with a serological survey of the Japanese general population. METHODS: Real-time polymerase chain reaction (PCR) and reverse transcription-PCR were used to detect the SERVagm-Vero genome. We also examined the seroprevalence of SERV in 1000 individuals in the Japanese population with an enzyme-linked immunoabsorbent assay (ELISA) using mixed SERVagm-Vero gag, pol, and env proteins as antigens. RESULTS: Real-time PCR failed to detect SERVagm-Vero genomic fragments in 783 human clinicopathological samples, and all 13 human cell lines tested were negative for the SERVagm-Vero genome. Thirteen commercialized vaccines, including five Vero-based vaccines, were also negative for the SERVagm-Vero genome on real-time PCR and reverse transcription-PCR. Eight (0.8%) were seropositive on ELISA, and western blotting showed that all eight sera contained anti-pol antibodies. All SERV-seropositive individuals were born before 1965, suggesting that SERV infection in Japan might not be associated with vaccine, because more than 90% of Japanese children born from 1964 to 2012 have received live poliovirus vaccines containing virus produced in Vero cells since the 1980s. CONCLUSION: We have confirmed that the vaccines we use today are free of SERVagm-Vero. Moreover, SERV infection in humans is very rare and unlikely to be associated with vaccines in the Japanese general population.
[Mh] Termos MeSH primário: Retrovirus Endógenos/isolamento & purificação
Genoma Viral
Retrovirus dos Símios/isolamento & purificação
Células Vero/virologia
Vacinas Virais/análise
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Animais
Anticorpos Antivirais/sangue
Linhagem Celular
Cercopithecus aethiops
Criança
Pré-Escolar
Contaminação de Medicamentos
Seres Humanos
Lactente
Recém-Nascido
Japão
Meia-Idade
Estudos Soroepidemiológicos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Viral Vaccines)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160427
[St] Status:MEDLINE


  4 / 211 MEDLINE  
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[PMID]:26364986
[Au] Autor:Sato K; Kobayashi T; Misawa N; Yoshikawa R; Takeuchi JS; Miura T; Okamoto M; Yasunaga J; Matsuoka M; Ito M; Miyazawa T; Koyanagi Y
[Ad] Endereço:Laboratory of Viral Pathogenesis, Institute for Virus Research, Kyoto University, Kyoto, Japan.
[Ti] Título:Experimental evaluation of the zoonotic infection potency of simian retrovirus type 4 using humanized mouse model.
[So] Source:Sci Rep;5:14040, 2015 Sep 14.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:During 2001-2002 and 2008-2011, two epidemic outbreaks of infectious hemorrhagic disease have been found in Japanese macaques (Macaca fuscata) in Kyoto University Primate Research Institute, Japan. Following investigations revealed that the causative agent was simian retrovirus type 4 (SRV-4). SRV-4 was isolated by using human cell lines, which indicates that human cells are potently susceptible to SRV-4 infection. These raise a possibility of zoonotic infection of pathogenic SRV-4 from Japanese macaques into humans. To explore the possibility of zoonotic infection of SRV-4 to humans, here we use a human hematopoietic stem cell-transplanted humanized mouse model. Eight out of the twelve SRV-4-inoculated humanized mice were infected with SRV-4. Importantly, 3 out of the 8 infected mice exhibited anemia and hemophagocytosis, and an infected mouse died. To address the possibility that SRV-4 adapts humanized mouse and acquires higher pathogenicity, the virus was isolated from an infected mice exhibited severe anemia was further inoculated into another 6 humanized mice. However, no infected mice exhibited any illness. Taken together, our findings demonstrate that the zoonotic SRV-4 infection from Japanese macaques to humans is technically possible under experimental condition. However, such zoonotic infection may not occur in the real society.
[Mh] Termos MeSH primário: Infecções por Retroviridae/transmissão
Retrovirus dos Símios/patogenicidade
Zoonoses/transmissão
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Citocinas/metabolismo
Citosina Desaminase/metabolismo
Feminino
Células HEK293
Transplante de Células-Tronco Hematopoéticas
Células-Tronco Hematopoéticas/citologia
Células-Tronco Hematopoéticas/metabolismo
Células-Tronco Hematopoéticas/virologia
Seres Humanos
Japão
Masculino
Camundongos
Camundongos Endogâmicos NOD
Camundongos Transgênicos
Modelos Animais
Reação em Cadeia da Polimerase
RNA Viral/análise
Infecções por Retroviridae/patologia
Infecções por Retroviridae/virologia
Retrovirus dos Símios/genética
Retrovirus dos Símios/isolamento & purificação
Transplante Heterólogo
Zoonoses/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokines); 0 (RNA, Viral); EC 3.5.4.1 (APOBEC3 protein, human); EC 3.5.4.1 (Cytosine Deaminase)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150915
[St] Status:MEDLINE
[do] DOI:10.1038/srep14040


  5 / 211 MEDLINE  
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[PMID]:26190584
[Au] Autor:Narahara C; Yasuda J
[Ad] Endereço:Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN).
[Ti] Título:Roles of the three L-domains in ß-retrovirus budding.
[So] Source:Microbiol Immunol;59(9):545-54, 2015 Sep.
[Is] ISSN:1348-0421
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:Retroviral Gag protein plays a critical role during the late stage of virus budding and possesses a so-called L-domain containing PT/SAP, PPxY, YxxL or FPIV motifs that are critical for efficient budding. Mason-Pfizer monkey virus (M-PMV) contains PSAP, PPPY, and YADL sequences in Gag. This study was performed to investigate the roles of these three L-domain-like sequences in virus replication in three different cell lines, 293T, COS-7 and HeLa cells. It was found that the PPxY motif plays an essential role in progeny virus production as a major L-domain in all three cell lines. The PSAP sequence was shown to function as an additional L-domain in HeLa cells and to promote efficient release of M-PMV; however, this sequence was dispensable for M-PMV production in 293T and COS-7 cells, suggesting that the role of the PSAP motif as an L-domain in M-PMV budding is cell type-dependent. Viruses possessing multiple L-domains appear to change the L-domain usage to replicate in various cells. On the other hand, the YADL motif was required for M-PMV production as a transport signal of Gag to the plasma membrane, but not as an L-domain.
[Mh] Termos MeSH primário: Produtos do Gene gag/metabolismo
Retrovirus dos Símios/fisiologia
Liberação de Vírus
[Mh] Termos MeSH secundário: Motivos de Aminoácidos
Animais
Linhagem Celular
Seres Humanos
Estrutura Terciária de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Gene Products, gag)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:150909
[Lr] Data última revisão:
150909
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150721
[St] Status:MEDLINE
[do] DOI:10.1111/1348-0421.12285


  6 / 211 MEDLINE  
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[PMID]:25743183
[Au] Autor:Okamoto M; Miyazawa T; Morikawa S; Ono F; Nakamura S; Sato E; Yoshida T; Yoshikawa R; Sakai K; Mizutani T; Nagata N; Takano J; Okabayashi S; Hamano M; Fujimoto K; Nakaya T; Iida T; Horii T; Miyabe-Nishiwaki T; Watanabe A; Kaneko A; Saito A; Matsui A; Hayakawa T; Suzuki J; Akari H; Matsuzawa T; Hirai H
[Ad] Endereço:Center for Human Evolution Modeling Research, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan.
[Ti] Título:Emergence of infectious malignant thrombocytopenia in Japanese macaques (Macaca fuscata) by SRV-4 after transmission to a novel host.
[So] Source:Sci Rep;5:8850, 2015 Mar 06.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We discovered a lethal hemorrhagic syndrome arising from severe thrombocytopenia in Japanese macaques kept at the Primate Research Institute, Kyoto University. Extensive investigation identified that simian retrovirus type 4 (SRV-4) was the causative agent of the disease. SRV-4 had previously been isolated only from cynomolgus macaques in which it is usually asymptomatic. We consider that the SRV-4 crossed the so-called species barrier between cynomolgus and Japanese macaques, leading to extremely severe acute symptoms in the latter. Infectious agents that cross the species barrier occasionally amplify in virulence, which is not observed in the original hosts. In such cases, the new hosts are usually distantly related to the original hosts. However, Japanese macaques are closely related to cynomolgus macaques, and can even hybridize when given the opportunity. This lethal outbreak of a novel pathogen in Japanese macaques highlights the need to modify our expectations about virulence with regards crossing species barriers.
[Mh] Termos MeSH primário: Doenças Transmissíveis Emergentes/complicações
Doenças Transmissíveis Emergentes/virologia
Infecções por Retroviridae/complicações
Infecções por Retroviridae/virologia
Retrovirus dos Símios/classificação
Retrovirus dos Símios/genética
Trombocitopenia/etiologia
[Mh] Termos MeSH secundário: Animais
Doenças Transmissíveis Emergentes/diagnóstico
Doenças Transmissíveis Emergentes/transmissão
Feminino
Genoma Viral
Macaca
Metagenômica/métodos
Filogenia
RNA Viral
Infecções por Retroviridae/diagnóstico
Infecções por Retroviridae/transmissão
Retrovirus dos Símios/isolamento & purificação
Retrovirus dos Símios/ultraestrutura
Trombocitopenia/diagnóstico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:150311
[Lr] Data última revisão:
150311
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150307
[St] Status:MEDLINE
[do] DOI:10.1038/srep08850


  7 / 211 MEDLINE  
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[PMID]:25267831
[Au] Autor:Osada N; Kohara A; Yamaji T; Hirayama N; Kasai F; Sekizuka T; Kuroda M; Hanada K
[Ad] Endereço:Division of Evolutionary Genetics, Department of Population Genetics, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan.
[Ti] Título:The genome landscape of the african green monkey kidney-derived vero cell line.
[So] Source:DNA Res;21(6):673-83, 2014 Dec.
[Is] ISSN:1756-1663
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Continuous cell lines that originate from mammalian tissues serve as not only invaluable tools for life sciences, but also important animal cell substrates for the production of various types of biological pharmaceuticals. Vero cells are susceptible to various types of microbes and toxins and have widely contributed to not only microbiology, but also the production of vaccines for human use. We here showed the genome landscape of a Vero cell line, in which 25,877 putative protein-coding genes were identified in the 2.97-Gb genome sequence. A homozygous ∼9-Mb deletion on chromosome 12 caused the loss of the type I interferon gene cluster and cyclin-dependent kinase inhibitor genes in Vero cells. In addition, an ∼59-Mb loss of heterozygosity around this deleted region suggested that the homozygosity of the deletion was established by a large-scale conversion. Moreover, a genomic analysis of Vero cells revealed a female Chlorocebus sabaeus origin and proviral variations of the endogenous simian type D retrovirus. These results revealed the genomic basis for the non-tumourigenic permanent Vero cell lineage susceptible to various pathogens and will be useful for generating new sub-lines and developing new tools in the quality control of Vero cells.
[Mh] Termos MeSH primário: Aberrações Cromossômicas
Genoma
Família Multigênica
Provírus/genética
Retrovirus dos Símios/genética
[Mh] Termos MeSH secundário: Animais
Cercopithecus aethiops
Feminino
Seres Humanos
Células Vero
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1507
[Cu] Atualização por classe:151029
[Lr] Data última revisão:
151029
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141001
[St] Status:MEDLINE
[do] DOI:10.1093/dnares/dsu029


  8 / 211 MEDLINE  
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[PMID]:24981450
[Au] Autor:Mohan M; Chandra LC; Torben W; Aye PP; Alvarez X; Lackner AA
[Ad] Endereço:Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA 70433 mmohan@tulane.edu.
[Ti] Título:miR-190b is markedly upregulated in the intestine in response to simian immunodeficiency virus replication and partly regulates myotubularin-related protein-6 expression.
[So] Source:J Immunol;193(3):1301-13, 2014 Aug 01.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:HIV replication and the cellular micro-RNA (miRNA) machinery interconnect at several posttranscriptional levels. To understand their regulatory role in the intestine, a major site of HIV/SIV replication, dissemination, and CD4(+) T cell depletion, we profiled miRNA expression in colon following SIV infection (10 acute SIV, 5 uninfected). Nine (four up and five down) miRNAs showed statistically significant differential expression. Most notably, miR-190b expression showed high statistical significance (adjusted p = 0.0032), the greatest fold change, and was markedly elevated in colon and jejunum throughout SIV infection. In addition, miR-190b upregulation was detected before peak viral replication and the nadir of CD4(+) T cell depletion predominantly in lamina propria leukocytes. Interestingly non-SIV-infected macaques with diarrhea and colitis failed to upregulate miR-190b, suggesting that its upregulation was neither inflammation nor immune-activation driven. SIV infection of in vitro-cultured CD4(+) T cells and primary intestinal macrophages conclusively identified miR-190b upregulation to be driven in response to viral replication. Further miR-190b expression levels in colon and jejunum positively correlated with tissue viral loads. In contrast, mRNA expression of myotubularin-related protein 6 (MTMR6), a negative regulator of CD4(+) T cell activation/proliferation, significantly decreased in SIV-infected macrophages. Luciferase reporter assays confirmed MTMR6 as a direct miR-190b target. To our knowledge, this is the first report, which describes dysregulated miRNA expression in the intestine, that identifies a potentially significant role for miR-190b in HIV/SIV pathogenesis. More importantly, miR-190b-mediated MTMR6 downregulation suggests an important mechanism that could keep infected cells in an activated state, thereby promoting viral replication. In the future, the mechanisms driving miR-190b upregulation including other cellular processes it regulates in SIV-infected cells need determination.
[Mh] Termos MeSH primário: Mucosa Intestinal/metabolismo
MicroRNAs/genética
Proteínas Tirosina Fosfatases não Receptoras/genética
Retrovirus dos Símios/genética
Síndrome de Imunodeficiência Adquirida dos Símios/genética
Vírus da Imunodeficiência Símia/genética
Regulação para Cima/genética
Replicação Viral/genética
[Mh] Termos MeSH secundário: Animais
Linfócitos T CD4-Positivos/imunologia
Linfócitos T CD4-Positivos/virologia
Colo/imunologia
Colo/metabolismo
Colo/virologia
Regulação para Baixo/genética
Regulação para Baixo/imunologia
Genes Reporter
Mucosa Intestinal/imunologia
Mucosa Intestinal/virologia
Jejuno/imunologia
Jejuno/metabolismo
Jejuno/virologia
Luciferases/genética
Macaca mulatta
MicroRNAs/biossíntese
Proteínas Tirosina Fosfatases não Receptoras/biossíntese
RNA Viral/genética
RNA Viral/imunologia
Retrovirus dos Símios/imunologia
Síndrome de Imunodeficiência Adquirida dos Símios/imunologia
Vírus da Imunodeficiência Símia/imunologia
Regulação para Cima/imunologia
Replicação Viral/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (MicroRNAs); 0 (RNA, Viral); EC 1.13.12.- (Luciferases); EC 3.1.3.48 (Protein Tyrosine Phosphatases, Non-Receptor)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:140702
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1303479


  9 / 211 MEDLINE  
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[PMID]:23612320
[Au] Autor:Ayouba A; Duval L; Liégeois F; Ngin S; Ahuka-Mundeke S; Switzer WM; Delaporte E; Ariey F; Peeters M; Nerrienet E
[Ad] Endereço:UM1 233, Institut de Recherche pour le Développement-IRD and University of Montpellier 1, Montpellier, France. ahidjo.ayouba@ird.fr
[Ti] Título:Nonhuman primate retroviruses from Cambodia: high simian foamy virus prevalence, identification of divergent STLV-1 strains and no evidence of SIV infection.
[So] Source:Infect Genet Evol;18:325-34, 2013 Aug.
[Is] ISSN:1567-7257
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Nonhuman primates (NHPs) carry retroviruses such as simian immunodeficiency viruses (SIV), simian T-cell lymphotropic viruses (STLV) and simian foamy viruses (SFV). Here, we revisited NHPs from Cambodia to assess the prevalence and diversity of these retroviruses using updated viral detection tools. We screened blood from 118 NHPs consisting of six species (Macaca fascicularis (n=91), Macaca leonine (n=8), Presbytis cristata (n=3), Nycticebus coucang (n=1), Hylobates pileatus (n=14), and Pongo pygmaeus) (n=1) by using a Luminex-based multiplex serology assay that allows the detection of all known SIV/HIV and SFV lineages. We also used highly sensitive PCR assays to detect each simian retrovirus group. Positive PCR products were sequenced and phylogenetically analyzed to infer evolutionary histories. Fifty-three of 118 (44.9%) NHPs tested positive for SFV by serology and 8/52 (15.4%), all from M. fascicularis, were PCR-confirmed. The 8 novel SFV sequences formed a highly supported distinct lineage within a clade composed of other macaque SFV. We observed no serological or molecular evidence of SIV infection among the 118 NHP samples tested. Four of 118 (3.3%) NHPs were PCR-positive for STLV, including one M. fascicularis, one P. cristata, and two H. pileatus. Phylogenetic analyses revealed that the four novel STLV belonged to the PTLV-1 lineage, outside the African radiation of PTLV-1, like all Asian PTLV identified so far. Sequence analysis of the whole STLV-1 genome from a H. pileatus (C578_Hp) revealed a genetic structure characteristic of PTLV. Similarity analysis comparing the STLV-1 (C578_Hp) sequence with prototype PTLVs showed that C578_Hp is closer to PTLV-1s than to all other types across the entire genome. In conclusion, we showed a high frequency of SFV infection but found no evidence of SIV infection in NHPs from Cambodia. We identified for the first time STLV-1 in a P. cristata and in two H. pileatus.
[Mh] Termos MeSH primário: Catarrinos/virologia
Doenças dos Primatas/virologia
Infecções por Retroviridae/veterinária
Retrovirus dos Símios/classificação
Vírus Espumoso dos Símios/classificação
Infecções Tumorais por Vírus/veterinária
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/sangue
Camboja
DNA Viral/sangue
Lorisidae/virologia
Dados de Sequência Molecular
Filogenia
Prevalência
Infecções por Retroviridae/sangue
Infecções por Retroviridae/virologia
Retrovirus dos Símios/genética
Retrovirus dos Símios/isolamento & purificação
Vírus Espumoso dos Símios/genética
Vírus Espumoso dos Símios/isolamento & purificação
Infecções Tumorais por Vírus/sangue
Infecções Tumorais por Vírus/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (DNA, Viral)
[Em] Mês de entrada:1404
[Cu] Atualização por classe:130729
[Lr] Data última revisão:
130729
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130425
[St] Status:MEDLINE


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[PMID]:23365453
[Au] Autor:Togami H; Shimura K; Okamoto M; Yoshikawa R; Miyazawa T; Matsuoka M
[Ad] Endereço:Laboratory of Virus Control, Institute for Virus Research, Kyoto University, Kyoto, Japan.
[Ti] Título:Comprehensive in vitro analysis of simian retrovirus type 4 susceptibility to antiretroviral agents.
[So] Source:J Virol;87(8):4322-9, 2013 Apr.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Simian retrovirus type 4 (SRV-4), a simian type D retrovirus, naturally infects cynomolgus monkeys, usually without apparent symptoms. However, some infected monkeys presented with an immunosuppressive syndrome resembling that induced by simian immunodeficiency virus infection. Antiretrovirals with inhibitory activity against SRV-4 are considered to be promising agents to combat SRV-4 infection. However, although some antiretrovirals have been reported to have inhibitory activity against SRV-1 and SRV-2, inhibitors with anti-SRV-4 activity have not yet been studied. In this study, we identified antiretroviral agents with anti-SRV-4 activity from a panel of anti-human immunodeficiency virus (HIV) drugs using a robust in vitro luciferase reporter assay. Among these, two HIV reverse transcriptase inhibitors, zidovudine (AZT) and tenofovir disoproxil fumarate (TDF), potently inhibited SRV-4 infection within a submicromolar to nanomolar range, which was similar to or higher than the activities against HIV-1, Moloney murine leukemia virus, and feline immunodeficiency virus. In contrast, nonnucleoside reverse transcriptase inhibitors and protease inhibitors did not exhibit any activities against SRV-4. Although both AZT and TDF effectively inhibited cell-free SRV-4 transmission, they exhibited only partial inhibitory activities against cell-to-cell transmission. Importantly, one HIV integrase strand transfer inhibitor, raltegravir (RAL), potently inhibited single-round infection as well as cell-free and cell-to-cell SRV-4 transmission. These findings indicate that viral expansion routes impact the inhibitory activity of antiretrovirals against SRV-4, while only RAL is effective in suppressing both the initial SRV-4 infection and subsequent SRV-4 replication.
[Mh] Termos MeSH primário: Antirretrovirais/farmacologia
Retrovirus dos Símios/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adenina/análogos & derivados
Adenina/farmacologia
Sequência de Aminoácidos
Animais
Genes Reporter
Integrases/genética
Luciferases/análise
Luciferases/genética
Testes de Sensibilidade Microbiana/métodos
Organofosfonatos/farmacologia
Inibidores de Proteases/farmacologia
DNA Polimerase Dirigida por RNA/genética
Inibidores da Transcriptase Reversa/farmacologia
Alinhamento de Sequência
Tenofovir
Zidovudina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Retroviral Agents); 0 (Organophosphonates); 0 (Protease Inhibitors); 0 (Reverse Transcriptase Inhibitors); 4B9XT59T7S (Zidovudine); 99YXE507IL (Tenofovir); EC 1.13.12.- (Luciferases); EC 2.7.7.- (Integrases); EC 2.7.7.49 (RNA-Directed DNA Polymerase); JAC85A2161 (Adenine)
[Em] Mês de entrada:1305
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130201
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.03208-12



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