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  1 / 6206 MEDLINE  
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[PMID]:28468626
[Au] Autor:Xu Q; Liu H; Yuan P; Zhang X; Chen Q; Jiang X; Zhou Y
[Ad] Endereço:Institute of Plant Protection; Jiangsu Academy of Agricultural Sciences; Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing, Jiangsu, People's Republic of China.
[Ti] Título:Development of a simplified RT-PCR without RNA isolation for rapid detection of RNA viruses in a single small brown planthopper (Laodelphax striatellus Fallén).
[So] Source:Virol J;14(1):90, 2017 05 03.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The small brown planthopper (SBPH) is an important pest of cereal crops and acts as a transmission vector for multiple RNA viruses. Rapid diagnosis of virus in the vector is crucial for efficient forecast and control of viral disease. Reverse transcription polymerase chain reaction (RT-PCR) is a rapid, sensitive and reliable method for virus detection. The traditional RT-PCR contains a RNA isolation step and is widely used for virus detection in insect. However, using the traditional RT-PCR for detecting RNA virus in individual SBPHs becomes challenging because of the expensive reagents and laborious procedure associated with RNA isolation when processing a large number of samples. RESULTS: We established a simplified RT-PCR method without RNA isolation for RNA virus detection in a single SBPH. This method is achieved by grinding a single SBPH in sterile water and using the crude extract directly as the template for RT-PCR. The crude extract containing the virus RNA can be prepared in approximately two minutes. Rice stripe virus (RSV), rice black streaked dwarf virus (RBSDV) and Himetobi P virus (HiPV) were successfully detected using this simplified method. The detection results were validated by sequencing and dot immunobinding assay, indicating that this simplified method is reliable for detecting different viruses in insects. The evaluation of the sensitivity of this method showed that both RSV and HiPV can be detected when the cDNA from the crude extract was diluted up to 10 fold. Compared to the traditional RT-PCR with RNA isolation, the simplified RT-PCR method greatly reduces the sample processing time, decreases the detection cost, and improves the efficiency by avoiding RNA isolation. CONCLUSIONS: A simplified RT-PCR method is developed for rapid detection of RNA virus in a single SBPH without the laborious RNA isolation step. It offers a convenient alternative to the traditional RT-PCR method.
[Mh] Termos MeSH primário: Hemípteros/virologia
Insetos Vetores/virologia
Vírus de RNA/isolamento & purificação
RNA Viral/isolamento & purificação
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
[Mh] Termos MeSH secundário: Animais
DNA Complementar/isolamento & purificação
Dicistroviridae/genética
Dicistroviridae/isolamento & purificação
Immunoblotting/métodos
Insetos/virologia
Vírus de Plantas/genética
Vírus de Plantas/isolamento & purificação
RNA Viral/análise
Reoviridae/genética
Reoviridae/isolamento & purificação
Sensibilidade e Especificidade
Tenuivirus/genética
Tenuivirus/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (RNA, Viral)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1186/s12985-017-0732-6


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[PMID]:29054449
[Au] Autor:Jiang Z; Li Z; Yue N; Zhang K; Li D; Zhang Y
[Ad] Endereço:State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, P.R. China.
[Ti] Título:Construction of infectious clones of lychnis ringspot virus and evaluation of its relationship with barley stripe mosaic virus by reassortment of genomic RNA segments.
[So] Source:Virus Res;243:106-109, 2018 01 02.
[Is] ISSN:1872-7492
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Lychnis ringspot virus (LRSV, genus Hordeivirus) was first isolated in 1959, and has been shown to infect several dicot plants in nature. However, due to the lack of infectious cDNA clones, the biological properties and mechanisms underlying LRSV infection are obscure. In this work, we constructed infectious cDNA clones of LRSV and have compiled the complete LRSV genomic (g) RNA sequence. Comparison of nucleotide and amino acid sequences between LRSV and barley stripe mosaic virus (BSMV), the type member of genus Hordeivirus, reveals that despite belonging to the same genus, and replicating in chloroplasts, the viruses are only distantly related. This could be further indicated by the failure of different LRSV/BSMV reassortants to infect N. benthamiana. LRSV infectious cDNA clones provide a useful tool for studies of biological diversity among hordeiviruses, and also may contribute to the understanding of seed transmission in dicot plants.
[Mh] Termos MeSH primário: Lychnis/virologia
Doenças das Plantas/virologia
Vírus de Plantas/genética
Vírus de RNA/genética
RNA Viral/genética
Recombinação Genética
[Mh] Termos MeSH secundário: Genoma Viral
Hordeum/virologia
Vírus de Plantas/classificação
Vírus de Plantas/isolamento & purificação
Vírus de Plantas/fisiologia
Vírus de RNA/classificação
Vírus de RNA/isolamento & purificação
Vírus de RNA/fisiologia
RNA Viral/metabolismo
Vírus Reordenados/classificação
Vírus Reordenados/genética
Vírus Reordenados/isolamento & purificação
Vírus Reordenados/fisiologia
Proteínas Virais/genética
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Viral); 0 (Viral Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171022
[St] Status:MEDLINE


  3 / 6206 MEDLINE  
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[PMID]:29050971
[Au] Autor:Zhou C; Li S; Zhou Y; Fan Y
[Ad] Endereço:College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China; Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
[Ti] Título:A simple method for obtaining rice black-streaked dwarf virus-infected small brown planthopper nymphs.
[So] Source:J Virol Methods;251:80-82, 2018 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Rice black-streaked dwarf virus (RBSDV), an important rice virus, is transmitted by vector small brown planthopper (SBPH) in a persistent manner, but not transovarial transmission. In order to obtain viruliferous SBPH nymphs for relevant research, a simple and reliable method was developed, through allowing SBPH adults laying eggs on RBSDV-infected rice plants. The results showed the hatching nymphs on diseased plants could early acquire virus, and the virus was detected in 2nd-instar nymphs from the spawning method, which was earlier than insect feed on diseased plant. The average viruliferous rate of SBPH from the spawning method was 32.9%, which was not lower than the feeding diseased plant method. The novel method was very easy to operate and time-saving, facilitating the study on the interaction between RBSDV and SBPH nymphs (especially young 2nd-4th instar nymphs), such as, the effect of RBSDV on nymph development, host plant orientation preference of viruliferous nymph, identification of viral interacting protein in nymph, etc.
[Mh] Termos MeSH primário: Entomologia/métodos
Hemípteros/virologia
Ninfa/virologia
Vírus de Plantas/crescimento & desenvolvimento
Virologia/métodos
[Mh] Termos MeSH secundário: Animais
Hemípteros/crescimento & desenvolvimento
Ninfa/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171021
[St] Status:MEDLINE


  4 / 6206 MEDLINE  
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[PMID]:28471518
[Au] Autor:Li S; Zhou C; Chen G; Zhou Y
[Ad] Endereço:Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
[Ti] Título:Bacterial microbiota in small brown planthopper populations with different rice viruses.
[So] Source:J Basic Microbiol;57(7):590-596, 2017 Jul.
[Is] ISSN:1521-4028
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The small brown planthopper (SBPH) is an important virus vector, transmitting Rice stripe virus (RSV), and Rice black-streaked dwarf virus (RBSDV). Insect symbionts play an essential role in the insect fitness, however, it is still unclear about their contributions to viral transmission by SBPH. Here, we investigated endosymbiont communities in non-viruliferous, RSV-infected, and RBSDV-infected SBPH populations using Illumina 16S rRNA gene MiSeq sequencing. In total, 281,803 effective sequences of the 16S rRNA gene were generated from different samples. Sequence analysis revealed the percentages of these bacterial groups in different SBPH populations on several taxonomic levels ranging from phyla to genera. The extremely consistent bacterial diversity and abundance indicated that RSV or RBSDV infection did not affect the composition and abundance of symbionts in SBPH. It was notable that Wolbachia was dominant in all populations. The symbiosis between Wolbachia and SBPH might be potentially studied and utilized to control pest SBPH in the future.
[Mh] Termos MeSH primário: Bactérias/isolamento & purificação
Hemípteros/microbiologia
Insetos Vetores/microbiologia
Microbiota
Simbiose
[Mh] Termos MeSH secundário: Animais
Bactérias/classificação
Bactérias/genética
Hemípteros/virologia
Sequenciamento de Nucleotídeos em Larga Escala
Insetos Vetores/virologia
Microbiota/genética
Oryza
Vírus de Plantas/isolamento & purificação
Vírus de Plantas/fisiologia
RNA Ribossômico 16S
Tenuivirus/isolamento & purificação
Tenuivirus/fisiologia
Wolbachia/genética
Wolbachia/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180117
[Lr] Data última revisão:
180117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1002/jobm.201700004


  5 / 6206 MEDLINE  
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[PMID]:27773769
[Au] Autor:Dietzgen RG; Kondo H; Goodin MM; Kurath G; Vasilakis N
[Ad] Endereço:Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, Queensland 4072, Australia. Electronic address: r.dietzgen@uq.edu.au.
[Ti] Título:The family Rhabdoviridae: mono- and bipartite negative-sense RNA viruses with diverse genome organization and common evolutionary origins.
[So] Source:Virus Res;227:158-170, 2017 01 02.
[Is] ISSN:1872-7492
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The family Rhabdoviridae consists of mostly enveloped, bullet-shaped or bacilliform viruses with a negative-sense, single-stranded RNA genome that infect vertebrates, invertebrates or plants. This ecological diversity is reflected by the diversity and complexity of their genomes. Five canonical structural protein genes are conserved in all rhabdoviruses, but may be overprinted, overlapped or interspersed with several novel and diverse accessory genes. This review gives an overview of the characteristics and diversity of rhabdoviruses, their taxonomic classification, replication mechanism, properties of classical rhabdoviruses such as rabies virus and rhabdoviruses with complex genomes, rhabdoviruses infecting aquatic species, and plant rhabdoviruses with both mono- and bipartite genomes.
[Mh] Termos MeSH primário: Evolução Molecular
Variação Genética
Genoma Viral
RNA Viral
Rhabdoviridae/classificação
Rhabdoviridae/fisiologia
[Mh] Termos MeSH secundário: Animais
Código de Barras de DNA Taxonômico
Interações Hospedeiro-Patógeno
Filogenia
Vírus de Plantas/classificação
Vírus de Plantas/fisiologia
Biossíntese de Proteínas
Vírus de RNA/classificação
Vírus de RNA/fisiologia
Transcrição Genética
Vertebrados
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE


  6 / 6206 MEDLINE  
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[PMID]:29036205
[Au] Autor:Liu JY; Fan HY; Wang Y; Zhang YL; Li DW; Yu JL; Han CG
[Ad] Endereço:State Key Laboratory for Agro-Biotechnology and Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing, P. R. China.
[Ti] Título:Characterization of microRNAs of Beta macrocarpa and their responses to Beet necrotic yellow vein virus infection.
[So] Source:PLoS One;12(10):e0186500, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plant microRNAs (miRNAs) are a class of non-coding RNAs that play important roles in plant development, defense, and symptom development. Here, 547 known miRNAs representing 129 miRNA families, and 282 potential novel miRNAs were identified in Beta macrocarpa using small RNA deep sequencing. A phylogenetic analysis was performed, and 8 Beta lineage-specific miRNAs were identified. Through a differential expression analysis, miRNAs associated with Beet necrotic yellow vein virus (BNYVV) infection were identified and confirmed using a microarray analysis and stem-loop RT-qPCR. In total, 103 known miRNAs representing 38 miRNA families, and 45 potential novel miRNAs were differentially regulated, with at least a two-fold change, in BNYVV-infected plants compared with that of the mock-inoculated control. Targets of these differentially expressed miRNAs were also predicted by degradome sequencing. These differentially expressed miRNAs were involved in hormone biosynthesis and signal transduction pathways, and enhanced axillary bud development and plant defenses. This work is the first to describe miRNAs of the plant genus Beta and may offer a reference for miRNA research in other species in the genus. It provides valuable information on the pathogenicity mechanisms of BNYVV.
[Mh] Termos MeSH primário: Beta vulgaris/genética
Beta vulgaris/virologia
MicroRNAs/genética
Doenças das Plantas/virologia
Vírus de Plantas/fisiologia
[Mh] Termos MeSH secundário: Beta vulgaris/citologia
Beta vulgaris/metabolismo
Regulação da Expressão Gênica de Plantas
Sequenciamento de Nucleotídeos em Larga Escala
Filogenia
Reguladores de Crescimento de Planta/biossíntese
Folhas de Planta/virologia
Análise de Sequência de RNA
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs); 0 (Plant Growth Regulators)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171017
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186500


  7 / 6206 MEDLINE  
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[PMID]:29020049
[Au] Autor:Gao R; Xu Y; Candresse T; He Z; Li S; Ma Y; Lu M
[Ad] Endereço:State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
[Ti] Título:Further insight into genetic variation and haplotype diversity of Cherry virus A from China.
[So] Source:PLoS One;12(10):e0186273, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cherry virus A (CVA) infection appears to be prevalent in cherry plantations worldwide. In this study, the diversity of CVA isolates from 31 cherry samples collected from different orchards around Bohai Bay in northeastern China was analyzed. The complete genome of one of these isolates, ChYT52, was found to be 7,434 nt in length excluding the poly (A) tail. It shares between 79.9-98.7% identity with CVA genome sequences in GenBank, while its RdRp core is more divergent (79.1-90.7% nt identity), likely as a consequence of a recombination event. Phylogenetic analysis of ChYT52 genome with CVA genomes in Genbank resulted in at least 7 major clusters plus additional 5 isolates alone at the end of long branches suggesting the existence of further phylogroups diversity in CVA. The genetic diversity of Chinese CVA isolates from 31 samples and GenBank sequences were analyzed in three genomic regions that correspond to the coat protein, the RNA-dependent RNA polymerase core region, and the movement protein genes. With few exceptions likely representing further recombination impact, the trees various trees are largely congruent, indicating that each region provides valuable phylogenetic information. In all cases, the majority of the Chinese CVA isolates clustering in phylogroup I, together with the X82547 reference sequence from Germany. Statistically significant negative values were obtained for Tajima's D in the three genes for phylogroup I, suggesting that it may be undergoing a period of expansion. There was considerable haplotype diversity in the individual samples and more than half samples contained genetically diverse haplotypes belonging to different phylogroups. In addition, a number of statistically significant recombination events were detected in CVA genomes or in the partial genomic sequences indicating an important contribution of recombination to CVA evolution. This work provides a foundation for elucidation of the epidemiological characteristics and evolutionary history of CVA populations.
[Mh] Termos MeSH primário: Variação Genética
Haplótipos/genética
Vírus de Plantas/genética
[Mh] Termos MeSH secundário: Sequência de Bases
China
Genoma Viral
Nucleotídeos/genética
Filogenia
Doenças das Plantas/virologia
Vírus de Plantas/isolamento & purificação
Prunus/virologia
Reação em Cadeia da Polimerase em Tempo Real
Recombinação Genética/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nucleotides)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171012
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186273


  8 / 6206 MEDLINE  
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[PMID]:28946005
[Au] Autor:Alam SB; Reade R; Theilmann J; Rochon D
[Ad] Endereço:Faculty of Land and Food Systems, University of British Columbia, Vancouver, B.C., Canada.
[Ti] Título:Evidence for the role of basic amino acids in the coat protein arm region of Cucumber necrosis virus in particle assembly and selective encapsidation of viral RNA.
[So] Source:Virology;512:83-94, 2017 Dec.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cucumber necrosis virus (CNV) is a T = 3 icosahedral virus with a (+)ssRNA genome. The N-terminal CNV coat protein arm contains a conserved, highly basic sequence ("KGRKPR"), which we postulate is involved in RNA encapsidation during virion assembly. Seven mutants were constructed by altering the CNV "KGRKPR" sequence; the four basic residues were mutated to alanine individually, in pairs, or in total. Virion accumulation and vRNA encapsidation were significantly reduced in mutants containing two or four substitutions and virion morphology was also affected, where both T = 1 and intermediate-sized particles were produced. Mutants with two or four substitutions encapsidated significantly greater levels of truncated RNA than that of WT, suggesting that basic residues in the "KGRKPR" sequence are important for encapsidation of full-length CNV RNA. Interestingly, "KGRKPR" mutants also encapsidated relatively higher levels of host RNA, suggesting that the "KGRKPR" sequence also contributes to selective encapsidation of CNV RNA.
[Mh] Termos MeSH primário: Aminoácidos Básicos/química
Proteínas do Capsídeo/metabolismo
Vírus de Plantas/metabolismo
RNA Viral/fisiologia
Montagem de Vírus/fisiologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas do Capsídeo/química
Proteínas do Capsídeo/genética
Mutação
Vírus de Plantas/genética
Conformação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids, Basic); 0 (Capsid Proteins); 0 (RNA, Viral)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE


  9 / 6206 MEDLINE  
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[PMID]:28869000
[Au] Autor:Lazareva EA; Lezzhov AA; Golyshev SA; Morozov SY; Heinlein M; Solovyev AG
[Ad] Endereço:1​Department of Virology, Biological Faculty, Moscow State University, Moscow, Russia.
[Ti] Título:Similarities in intracellular transport of plant viral movement proteins BMB2 and TGB3.
[So] Source:J Gen Virol;98(9):2379-2391, 2017 Sep.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The cell-to-cell transport of many plant viruses through plasmodesmata requires viral movement proteins (MPs) encoded by a 'triple gene block' (TGB) and termed TGB1, TGB2 and TGB3. TGB3 is a small integral membrane protein that contains subcellular targeting signals and directs both TGB2 and the helicase domain-containing TGB1 protein to plasmodesmata-associated structures. Recently, we described a 'binary movement block' (BMB) coding for two MPs, BMB1 and BMB2. The BMB2 protein associates with endoplasmic reticulum (ER) membranes, accumulates at plasmodesmata-associated membrane bodies and directs the BMB1 helicase to these structures. TGB3 transport to cell peripheral bodies was previously shown to bypass the secretory pathway and involve a non-conventional mechanism. Here, we provide evidence that the intracellular transport of both poa semilatent virus TGB3 and hibiscus green spot virus BMB2 to plasmodesmata-associated sites can occur via lateral translocation along the ER membranes. Agrobacterium-mediated transient co-expression in Nicotiana benthamiana leaves revealed that green fluorescent protein (GFP)-fused actin-binding domains of Arabidopsis fimbrin (ABD2-GFP) and mouse talin (TAL-GFP) inhibited the subcellular targeting of TGB3 and BMB2 to plasmodesmata-associated bodies, which resulted in TGB3 and BMB2 accumulation in the cytoplasm in association with aberrant ER structures. Inhibition of COPII budding complex formation by the expression of a dominant-negative mutant of the small GTPase Sar1 had no detectable effect on BMB2 subcellular targeting, which therefore could occur without exit from the ER in COPII transport vesicles. Collectively, the presented data support the current view that plant viral MPs exploit the ER:actin network for their intracellular transport.
[Mh] Termos MeSH primário: Proteínas do Movimento Viral em Plantas/metabolismo
Vírus de Plantas/metabolismo
[Mh] Termos MeSH secundário: Retículo Endoplasmático/metabolismo
Retículo Endoplasmático/virologia
Doenças das Plantas/virologia
Proteínas do Movimento Viral em Plantas/genética
Vírus de Plantas/genética
Transporte Proteico
Tabaco/virologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Viral Movement Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170905
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000914


  10 / 6206 MEDLINE  
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[PMID]:28866237
[Au] Autor:Del Toro FJ; Rakhshandehroo F; Larruy B; Aguilar E; Tenllado F; Canto T
[Ad] Endereço:Department of Environmental Biology, Center for Biological Research, CIB-CSIC, Ramiro de Maeztu 9, Madrid 28040, Spain. Electronic address: fjdts@cib.csic.es.
[Ti] Título:Effects of simultaneously elevated temperature and CO levels on Nicotiana benthamiana and its infection by different positive-sense RNA viruses are cumulative and virus type-specific.
[So] Source:Virology;511:184-192, 2017 Nov.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We have studied how simultaneously elevated temperature and CO levels [climate change-related conditions (CCC) of 30°C, 970 parts-per-million (ppm) of CO vs. standard conditions (SC) of 25°C, ~ 405ppm CO ] affect physiochemical properties of Nicotiana benthamiana leaves, and also its infection by several positive-sense RNA viruses. In previous works we had studied effects of elevated temperature, CO levels separately. Under CCC, leaves of healthy plants almost doubled their area relative to SC but contained less protein/unit-of-area, similarly to what we had found under conditions of elevated CO alone. CCC also affected the sizes/numbers of different foliar cell types differently. Under CCC, infection outcomes in titers and symptoms were virus type-specific, broadly similar to those observed under elevated temperature alone. Under either condition, infections did not significantly alter the protein content of leaf discs. Therefore, effects of elevated temperature and CO combined on properties of the pathosystems studied were overall cumulative.
[Mh] Termos MeSH primário: Dióxido de Carbono
Doenças das Plantas/virologia
Vírus de Plantas/crescimento & desenvolvimento
Vírus de RNA/crescimento & desenvolvimento
Tabaco/efeitos dos fármacos
Tabaco/efeitos da radiação
[Mh] Termos MeSH secundário: Fenômenos Químicos/efeitos dos fármacos
Fenômenos Químicos/efeitos da radiação
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/efeitos da radiação
Folhas de Planta/virologia
Temperatura Ambiente
Tabaco/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
142M471B3J (Carbon Dioxide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170904
[St] Status:MEDLINE



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