Base de dados : MEDLINE
Pesquisa : B04.715.081 [Categoria DeCS]
Referências encontradas : 32 [refinar]
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[PMID]:26147354
[Au] Autor:Farrow SC; Hagel JM; Beaudoin GA; Burns DC; Facchini PJ
[Ad] Endereço:Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada.
[Ti] Título:Stereochemical inversion of (S)-reticuline by a cytochrome P450 fusion in opium poppy.
[So] Source:Nat Chem Biol;11(9):728-32, 2015 Sep.
[Is] ISSN:1552-4469
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The gateway to morphine biosynthesis in opium poppy (Papaver somniferum) is the stereochemical inversion of (S)-reticuline since the enzyme yielding the first committed intermediate salutaridine is specific for (R)-reticuline. A fusion between a cytochrome P450 (CYP) and an aldo-keto reductase (AKR) catalyzes the S-to-R epimerization of reticuline via 1,2-dehydroreticuline. The reticuline epimerase (REPI) fusion was detected in opium poppy and in Papaver bracteatum, which accumulates thebaine. In contrast, orthologs encoding independent CYP and AKR enzymes catalyzing the respective synthesis and reduction of 1,2-dehydroreticuline were isolated from Papaver rhoeas, which does not accumulate morphinan alkaloids. An ancestral relationship between these enzymes is supported by a conservation of introns in the gene fusions and independent orthologs. Suppression of REPI transcripts using virus-induced gene silencing in opium poppy reduced levels of (R)-reticuline and morphinan alkaloids and increased the overall abundance of (S)-reticuline and its O-methylated derivatives. Discovery of REPI completes the isolation of genes responsible for known steps of morphine biosynthesis.
[Mh] Termos MeSH primário: Aldeído Redutase/metabolismo
Carboidratos Epimerases/metabolismo
Sistema Enzimático do Citocromo P-450/metabolismo
Regulação da Expressão Gênica de Plantas
Morfina/biossíntese
Papaver/metabolismo
Proteínas de Plantas/metabolismo
[Mh] Termos MeSH secundário: Aldeído Redutase/genética
Aldo-Ceto Redutases
Alcaloides/biossíntese
Alcaloides/química
Sequência de Bases
Benzilisoquinolinas/química
Benzilisoquinolinas/metabolismo
Bromoviridae/genética
Bromoviridae/metabolismo
Carboidratos Epimerases/antagonistas & inibidores
Carboidratos Epimerases/genética
Sistema Enzimático do Citocromo P-450/genética
Escherichia coli/genética
Escherichia coli/metabolismo
Éxons
Fusão Gênica
Íntrons
Ligases/genética
Ligases/metabolismo
Dados de Sequência Molecular
Morfinanos/química
Morfinanos/metabolismo
Morfina/química
Fases de Leitura Aberta
Ópio/química
Ópio/metabolismo
Oxirredução
Papaver/genética
Proteínas de Plantas/genética
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/metabolismo
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkaloids); 0 (Benzylisoquinolines); 0 (Morphinans); 0 (Plant Proteins); 0 (Recombinant Proteins); 76I7G6D29C (Morphine); 7X10PRH74D (salutaridine); 8008-60-4 (Opium); 9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.1.1.- (Aldo-Keto Reductases); EC 1.1.1.21 (Aldehyde Reductase); EC 5.1.3.- (Carbohydrate Epimerases); EC 6.- (Ligases); X35Z551WT4 (reticuline)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150707
[St] Status:MEDLINE
[do] DOI:10.1038/nchembio.1879


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[PMID]:25564866
[Au] Autor:Tubiana L; Bozic AL; Micheletti C; Podgornik R
[Ad] Endereço:Department of Theoretical Physics, Jozef Stefan Institute, Ljubljana, Slovenia. Electronic address: luca.tubiana@ijs.si.
[Ti] Título:Synonymous mutations reduce genome compactness in icosahedral ssRNA viruses.
[So] Source:Biophys J;108(1):194-202, 2015 Jan 06.
[Is] ISSN:1542-0086
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Recent studies have shown that single-stranded (ss) viral RNAs fold into more compact structures than random RNA sequences with similar chemical composition and identical length. Based on this comparison, it has been suggested that wild-type viral RNA may have evolved to be atypically compact so as to aid its encapsidation and assist the viral assembly process. To further explore the compactness selection hypothesis, we systematically compare the predicted sizes of >100 wild-type viral sequences with those of their mutants, which are evolved in silico and subject to a number of known evolutionary constraints. In particular, we enforce mutation synonynimity, preserve the codon-bias, and leave untranslated regions intact. It is found that progressive accumulation of these restricted mutations still suffices to completely erase the characteristic compactness imprint of the viral RNA genomes, making them in this respect physically indistinguishable from randomly shuffled RNAs. This shows that maintaining the physical compactness of the genome is indeed a primary factor among ssRNA viruses' evolutionary constraints, contributing also to the evidence that synonymous mutations in viral ssRNA genomes are not strictly neutral.
[Mh] Termos MeSH primário: Mutação Puntual
RNA Viral/química
[Mh] Termos MeSH secundário: Bromoviridae
Caliciviridae
Códon
Bases de Dados Genéticas
Evolução Molecular
Flaviviridae
Conformação de Ácido Nucleico
Picornaviridae
Secoviridae
Tombusviridae
Tymoviridae
Regiões não Traduzidas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Codon); 0 (RNA, Viral); 0 (Untranslated Regions)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150108
[St] Status:MEDLINE


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[PMID]:24838850
[Au] Autor:Scott SW; MacFarlane SA; McGavin WJ; Fargette D
[Ad] Endereço:Department of Biological Sciences, Clemson University, Clemson, SC, 29634-0314, USA, sscott@clemson.edu.
[Ti] Título:Cassava Ivorian bacilliform virus is a member of the genus Anulavirus.
[So] Source:Arch Virol;159(10):2791-3, 2014 Oct.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:The complete genomic sequence of Cassava Ivorian bacilliform virus (CIBV) is described. The virus has a genomic organization similar to that of pelargonium zonate spot virus (PZSV), the type member of the genus Anulavirus, but it is most closely related to a second, recently described, anulavirus, Amazon lily mild mottle virus (ALiMMV).
[Mh] Termos MeSH primário: Bromoviridae/classificação
Bromoviridae/genética
Genoma Viral/genética
Manihot/virologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sequência de Bases
Variação Genética
Dados de Sequência Molecular
Fases de Leitura Aberta/genética
Doenças das Plantas/virologia
RNA Viral/genética
Análise de Sequência de RNA
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral); 0 (Viral Proteins)
[Em] Mês de entrada:1411
[Cu] Atualização por classe:140924
[Lr] Data última revisão:
140924
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140520
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-014-2086-3


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[PMID]:23386292
[Au] Autor:Ramanna H; Ding XS; Nelson RS
[Ad] Endereço:Plant Biology Division, The Samuel Roberts Noble Foundation Inc., Ardmore, OK, USA.
[Ti] Título:Rationale for developing new virus vectors to analyze gene function in grasses through virus-induced gene silencing.
[So] Source:Methods Mol Biol;975:15-32, 2013.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The exploding availability of genome and EST-based sequences from grasses requires a technology that allows rapid functional analysis of the multitude of genes that these resources provide. There are several techniques available to determine a gene's function. For gene knockdown studies, silencing through RNAi is a powerful tool. Gene silencing can be accomplished through stable transformation or transient expression of a fragment of a target gene sequence. Stable transformation in rice, maize, and a few other species, although routine, remains a relatively low-throughput process. Transformation in other grass species is difficult and labor-intensive. Therefore, transient gene silencing methods including Agrobacterium-mediated and virus-induced gene silencing (VIGS) have great potential for researchers studying gene function in grasses. VIGS in grasses already has been used to determine the function of genes during pathogen challenge and plant development. It also can be used in moderate-throughput reverse genetics screens to determine gene function. However, the number of viruses modified to serve as silencing vectors in grasses is limited, and the silencing phenotype induced by these vectors is not optimal: the phenotype being transient and with moderate penetration throughout the tissue. Here, we review the most recent information available for VIGS in grasses and summarize the strengths and weaknesses in current virus-grass host systems. We describe ways to improve current virus vectors and the potential of other grass-infecting viruses for VIGS studies. This work is necessary because VIGS for the foreseeable future remains a higher throughput and more rapid system to evaluate gene function than stable transformation.
[Mh] Termos MeSH primário: Bromoviridae/genética
Genes de Plantas
Vírus do Mosaico/genética
Poaceae/genética
Interferência de RNA
[Mh] Termos MeSH secundário: Regulação da Expressão Gênica de Plantas
Técnicas de Silenciamento de Genes
Vetores Genéticos
Anotação de Sequência Molecular
Poaceae/virologia
Transdução Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Em] Mês de entrada:1307
[Cu] Atualização por classe:130206
[Lr] Data última revisão:
130206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130207
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-62703-278-0_2


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[PMID]:22965580
[Au] Autor:Fuji S; Kikuchi M; Ueda S; Toda T; Furuya H; Fukumoto F; Hanada K
[Ad] Endereço:Faculty of Bioresource Sciences, Akita Prefectural University, Shimoshinjo, Akita 010-0195, Japan. sfuji@akita-pu.ac.jp
[Ti] Título:Characterization of a new Anulavirus isolated from Amazon lily plants.
[So] Source:Arch Virol;158(1):201-6, 2013 Jan.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:A quasi-spherical virus was isolated from a cultivated Amazon lily plant (Eucharis grandiflora) that could be mechanically transmitted to healthy E. grandiflora plants, subsequently producing mild mosaic or mottle symptoms on the leaves. The purified virus consisted of three quasi-spherical particles about 20 nm wide and 70, 40 and 30 nm in length, containing three segmented genomes of 3,169, 2,507 and 2,530 nucleotides, respectively. Sequence analysis showed that the newly isolated virus is related to pelargonium zonate spot virus, a member of the genus Anulavirus. We propose that the virus should be designated as Amazon lily mild mottle virus (ALiMMV).
[Mh] Termos MeSH primário: Bromoviridae/genética
Bromoviridae/isolamento & purificação
Lilium/virologia
Doenças das Plantas/virologia
[Mh] Termos MeSH secundário: Bromoviridae/classificação
Genoma Viral
Dados de Sequência Molecular
Filogenia
Folhas de Planta/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1302
[Cu] Atualização por classe:130109
[Lr] Data última revisão:
130109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120912
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-012-1467-8


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[PMID]:21253783
[Au] Autor:Valasevich N; Kukharchyk N; Kvarnheden A
[Ad] Endereço:Department of Biotechnology, Institute for Fruit Growing, Samochvalovichi, Belarus. natallia.valasevich@vbsg.slu.se
[Ti] Título:Molecular characterisation of Raspberry bushy dwarf virus isolates from Sweden and Belarus.
[So] Source:Arch Virol;156(3):369-74, 2011 Mar.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:The complete coding sequences were determined for RNA-1 and RNA-2 of five raspberry isolates of Raspberry bushy dwarf virus (RBDV) from Belarus (BY1, BY3, BY8, BY22) and Sweden (SE3). The analysed sequences for both RNA-1 and RNA-2 were highly conserved among these isolates. Phylogenetic analyses including available sequences for the CP gene and the MP gene showed that all analysed RBDV isolates from raspberry were closely related. However, there was no strong correlation between the grouping of raspberry isolates in the phylogenetic analyses and their geographical location. In contrast, RBDV isolates showed a host-dependent relationship with isolates from raspberry and grapevine, forming two distinct clades.
[Mh] Termos MeSH primário: Bromoviridae/classificação
Bromoviridae/genética
Genoma Viral
Doenças das Plantas/virologia
RNA Viral/genética
Rosaceae/virologia
[Mh] Termos MeSH secundário: Bromoviridae/isolamento & purificação
Proteínas do Capsídeo/genética
Análise por Conglomerados
Sequência Conservada
Dados de Sequência Molecular
Filogenia
República da Bielorrússia
Análise de Sequência de DNA
Suécia
Proteínas da Matriz Viral/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (RNA, Viral); 0 (Viral Matrix Proteins)
[Em] Mês de entrada:1104
[Cu] Atualização por classe:110225
[Lr] Data última revisão:
110225
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110122
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-010-0912-9


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[PMID]:20626283
[Au] Autor:Lapidot M; Guenoune-Gelbart D; Leibman D; Holdengreber V; Davidovitz M; Machbash Z; Klieman-Shoval S; Cohen S; Gal-On A
[Ad] Endereço:Department of Vegetable Research, Volcani Center, ARO, Bet Dagan 50250, Israel. lapidotm@volcani.agri.gov.il
[Ti] Título:Pelargonium zonate spot virus is transmitted vertically via seed and pollen in tomato.
[So] Source:Phytopathology;100(8):798-804, 2010 Aug.
[Is] ISSN:0031-949X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In autumn 2007, a new disease with unknown etiology was observed in open-field tomato (Solanum lycopersicum) in the Lachish region of Israel. The symptoms included mild mosaic, leaf malformation, and severe stunting of the plants. The causal agent was readily transmitted mechanically from the sap of infected plants to indicator plants. Viral particles were purified from infected plants and cDNA was synthesized from RNA isolated from the particles. Cloning and sequencing of the cDNA showed 95% identity to RNA 3 of Pelargonium zonate spot virus (PZSV). Using reverse-transcription polymerase chain reaction, PZSV was detected in both seed and pollen grains of infected tomato plants. Attempts to disinfect seed by using hydrochloric acid and trisodium phosphate failed to eliminate this PZSV detection. Seed from infected tomato plants gave rise to infected seedlings with a seed-transmission rate of PZSV of 11 to 29%. Pollen grains collected from flowers of infected plants were used to hand pollinate healthy mother tomato plants. Although none of the pollinated mother plants became infected with PZSV, 29% of the seedlings produced from seed harvested from these plants were found to be infected. This is the first demonstration that PZSV is transmitted vertically via both pollen and seed in tomato plants.
[Mh] Termos MeSH primário: Bromoviridae/fisiologia
Interações Hospedeiro-Patógeno
Lycopersicon esculentum/virologia
Doenças das Plantas/virologia
[Mh] Termos MeSH secundário: Pólen/virologia
Sementes/virologia
Análise de Sequência de RNA
Microbiologia do Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1009
[Cu] Atualização por classe:100714
[Lr] Data última revisão:
100714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100715
[St] Status:MEDLINE
[do] DOI:10.1094/PHYTO-100-8-0798


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[PMID]:20170682
[Au] Autor:Luo H; Wylie SJ; Jones MG
[Ad] Endereço:Plant Biotechnology Research Group, Western Australian State Agricultural Biotechnology Centre, School of Biological Sciences and Biotechnology, Murdoch University, Perth, WA 6150, Australia.
[Ti] Título:Identification of plant viruses using one-dimensional gel electrophoresis and peptide mass fingerprints.
[So] Source:J Virol Methods;165(2):297-301, 2010 May.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A generic assay to detect and partially characterize unknown viruses from plants was developed. Proteins extracted from virus-infected and uninfected plants were separated in one dimension by SDS polyacrylamide gel electrophoresis. Differentially expressed protein bands were eluted after trypsin digestion and resulting peptide fragments separated according to their mass by matrix-assisted laser-desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Resulting peptide mass fingerprints (PMF) were compared with those in protein databases. The assay was used to identify three known viruses: the potyviruses Zucchini yellow mosaic virus and Turnip mosaic virus, and an alfamovirus Alfalfa mosaic virus. It was also used to identify a virus that manifested symptoms in wild Cakile maritima plants, tentatively identified as Pelargonium zonate spot virus (PZSV) (genus Anulavirus) by its PMF, and then confirmed by nucleotide sequencing. The detection of PZSV constitutes a first record of this virus in Australia and in this host. It is proposed that this rapid and simple assay is a useful approach for analysis of plant samples known to harbor viruses that could not be identified using antisera or nucleic acid-based assays.
[Mh] Termos MeSH primário: Alfamovirus/isolamento & purificação
Bromoviridae/isolamento & purificação
Eletroforese em Gel de Poliacrilamida
Mapeamento de Peptídeos/métodos
Doenças das Plantas/virologia
Potyvirus/isolamento & purificação
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
[Mh] Termos MeSH secundário: Alfamovirus/genética
Austrália
Sequência de Bases
Brassicaceae/virologia
Bromoviridae/genética
Proteínas do Capsídeo/química
Proteínas do Capsídeo/genética
Peso Molecular
Potyvirus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Capsid Proteins)
[Em] Mês de entrada:1007
[Cu] Atualização por classe:100407
[Lr] Data última revisão:
100407
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100223
[St] Status:MEDLINE
[do] DOI:10.1016/j.jviromet.2010.01.022


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[PMID]:20117141
[Au] Autor:Untiveros M; Perez-Egusquiza Z; Clover G
[Ad] Endereço:Plant Health and Environment Laboratory, Investigation and Diagnostic Centre, MAF Biosecurity New Zealand, PO Box 2095, Auckland 1140, New Zealand.
[Ti] Título:PCR assays for the detection of members of the genus Ilarvirus and family Bromoviridae.
[So] Source:J Virol Methods;165(1):97-104, 2010 Apr.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A PCR assay was developed for the universal detection of ilarviruses using primers designed to the RNA-dependent RNA polymerase gene in RNA2. The assay detected 32 isolates of 15 definite and 2 tentative ilarvirus species using a one-step RT-PCR. The assay was more specific, and at least as sensitive as a commercial assay, and allowed direct sequencing of amplicons. No cross-reaction was observed with neither healthy plants of 15 host species nor from isolates in other genera of the Bromoviridae. A further PCR assay targeting the helicase motif of RNA1 was able to detect all species tested within the family Bromoviridae, including members of the Alfamovirus, Anulavirus, Bromovirus, Cucumovirus and Ilarvirus. The assays provide a sensitive and cost-effective way for detecting and characterising members of the Bromoviridae and can be used for quarantine and certification programmes.
[Mh] Termos MeSH primário: Bromoviridae/genética
Bromoviridae/isolamento & purificação
RNA Viral/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
[Mh] Termos MeSH secundário: Bromoviridae/classificação
Reações Cruzadas
Primers do DNA/genética
Dados de Sequência Molecular
RNA Helicases/genética
RNA Replicase/genética
Sensibilidade e Especificidade
Análise de Sequência de DNA
Proteínas Virais/genética
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (RNA, Viral); 0 (Viral Proteins); EC 2.7.7.48 (RNA Replicase); EC 3.6.4.13 (RNA Helicases)
[Em] Mês de entrada:1005
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100202
[St] Status:MEDLINE
[do] DOI:10.1016/j.jviromet.2010.01.011


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[PMID]:19397928
[Au] Autor:Gulati-Sakhuja A; Sears JL; Nuñez A; Liu HY
[Ad] Endereço:USDA-ARS, Salinas, CA 93905, USA.
[Ti] Título:Production of polyclonal antibodies against Pelargonium zonate spot virus coat protein expressed in Escherichia coli and application for immunodiagnosis.
[So] Source:J Virol Methods;160(1-2):29-37, 2009 Sep.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Pelargonium zonate spot virus (PZSV) is identified recently in tomato plants in the United States. To develop serological diagnostic tools for the detection of this virus, the production of good quality antibodies is a necessity. The coat protein (CP) gene of a California isolate of PZSV was cloned into a bacterial expression vector (pTriEX-4 Ek/LIC). The plasmid pTriEX-4-PZSV-CP was transformed into Escherichia coli Rosetta 2(DE3)pLacI and the recombinant PZSV-CP was expressed as a fusion protein containing N-terminal hexa-histidine and S tags. Expressed PZSV-CP was purified under denaturing conditions by affinity chromatography yielding 3mg refolded protein per 200mL of bacterial culture, and used as an antigen for raising PZSV-CP antiserum in rabbits. Specificity of the antiserum to PZSV was shown by Western blot and ELISA. When used in Western blot analysis, the antiserum was able to detect the recombinant protein, the PZSV coat protein and PZSV infected plant samples. The antiserum was successfully used in indirect-ELISA at dilutions of up to 1:16,000 to detect PZSV in infected leaf samples. Direct ELISA was successful only with denatured antigens. This is the first report on production of polyclonal antiserum against recombinant coat protein of PZSV and its use for detection and diagnosis of virus using serological methods.
[Mh] Termos MeSH primário: Anticorpos Antivirais
Bromoviridae/imunologia
Proteínas do Capsídeo/imunologia
Testes Imunológicos/métodos
Lycopersicon esculentum/virologia
Doenças das Plantas/virologia
[Mh] Termos MeSH secundário: Animais
Western Blotting/métodos
Bromoviridae/genética
Proteínas do Capsídeo/genética
Ensaio de Imunoadsorção Enzimática/métodos
Escherichia coli/genética
Expressão Gênica
Coelhos
Proteínas Recombinantes/genética
Proteínas Recombinantes/imunologia
Sensibilidade e Especificidade
Estados Unidos
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Capsid Proteins); 0 (Recombinant Proteins)
[Em] Mês de entrada:0908
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090429
[St] Status:MEDLINE
[do] DOI:10.1016/j.jviromet.2009.04.005



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde