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Pesquisa : B04.715.081.050 [Categoria DeCS]
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  1 / 7 MEDLINE  
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[PMID]:20170682
[Au] Autor:Luo H; Wylie SJ; Jones MG
[Ad] Endereço:Plant Biotechnology Research Group, Western Australian State Agricultural Biotechnology Centre, School of Biological Sciences and Biotechnology, Murdoch University, Perth, WA 6150, Australia.
[Ti] Título:Identification of plant viruses using one-dimensional gel electrophoresis and peptide mass fingerprints.
[So] Source:J Virol Methods;165(2):297-301, 2010 May.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A generic assay to detect and partially characterize unknown viruses from plants was developed. Proteins extracted from virus-infected and uninfected plants were separated in one dimension by SDS polyacrylamide gel electrophoresis. Differentially expressed protein bands were eluted after trypsin digestion and resulting peptide fragments separated according to their mass by matrix-assisted laser-desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Resulting peptide mass fingerprints (PMF) were compared with those in protein databases. The assay was used to identify three known viruses: the potyviruses Zucchini yellow mosaic virus and Turnip mosaic virus, and an alfamovirus Alfalfa mosaic virus. It was also used to identify a virus that manifested symptoms in wild Cakile maritima plants, tentatively identified as Pelargonium zonate spot virus (PZSV) (genus Anulavirus) by its PMF, and then confirmed by nucleotide sequencing. The detection of PZSV constitutes a first record of this virus in Australia and in this host. It is proposed that this rapid and simple assay is a useful approach for analysis of plant samples known to harbor viruses that could not be identified using antisera or nucleic acid-based assays.
[Mh] Termos MeSH primário: Alfamovirus/isolamento & purificação
Bromoviridae/isolamento & purificação
Eletroforese em Gel de Poliacrilamida
Mapeamento de Peptídeos/métodos
Doenças das Plantas/virologia
Potyvirus/isolamento & purificação
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
[Mh] Termos MeSH secundário: Alfamovirus/genética
Austrália
Sequência de Bases
Brassicaceae/virologia
Bromoviridae/genética
Proteínas do Capsídeo/química
Proteínas do Capsídeo/genética
Peso Molecular
Potyvirus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Capsid Proteins)
[Em] Mês de entrada:1007
[Cu] Atualização por classe:100407
[Lr] Data última revisão:
100407
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100223
[St] Status:MEDLINE
[do] DOI:10.1016/j.jviromet.2010.01.022


  2 / 7 MEDLINE  
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[PMID]:16078876
[Au] Autor:Bol JF
[Ad] Endereço:Institute of Biology, Gorlaeus Laboratories, Leiden University, PO Box 9502, 2300 RA Leiden, The Netherlands. j.bol@chem.leidenuniv.nl
[Ti] Título:Replication of alfamo- and ilarviruses: role of the coat protein.
[So] Source:Annu Rev Phytopathol;43:39-62, 2005.
[Is] ISSN:0066-4286
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the family Bromoviridae, a mixture of the three genomic RNAs of bromo-, cucumo-, and oleaviruses is infectious as such, whereas the RNAs of alfamo- and ilarviruses require binding of a few molecules of coat protein (CP) to the 3' end to initiate infection. Most studies on the early function of CP have been done on the alfamovirus Alfalfa mosaic virus (AMV). The 3' 112 nucleotides of AMV RNAs can adopt two different conformations. One conformer consists of a tRNA-like structure that, together with an upstream hairpin, is required for minus-strand promoter activity. The other conformer consists of four hairpins interspersed by AUGC-sequences and represents a strong binding site for CP. Binding of CP to this conformer enhances the translational efficiency of viral RNAs in vivo 40-fold and blocks viral minus-strand RNA synthesis in vitro. AMV CP is proposed to initiate infection by mimicking the function of the poly(A)-binding protein.
[Mh] Termos MeSH primário: Alfamovirus/fisiologia
Proteínas do Capsídeo/metabolismo
Ilarvirus/fisiologia
Replicação Viral
[Mh] Termos MeSH secundário: Alfamovirus/genética
Proteínas do Capsídeo/genética
Regulação Viral da Expressão Gênica
Genoma Viral
Ilarvirus/genética
Doenças das Plantas/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Capsid Proteins)
[Em] Mês de entrada:0601
[Cu] Atualização por classe:050804
[Lr] Data última revisão:
050804
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:050805
[St] Status:MEDLINE


  3 / 7 MEDLINE  
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[PMID]:10836792
[Au] Autor:Haasnoot PC; Brederode FT; Olsthoorn RC; Bol JF
[Ad] Endereço:Institute of Molecular Plant Sciences, Gorlaeus Laboratories, Leiden University, The Netherlands.
[Ti] Título:A conserved hairpin structure in Alfamovirus and Bromovirus subgenomic promoters is required for efficient RNA synthesis in vitro.
[So] Source:RNA;6(5):708-16, 2000 May.
[Is] ISSN:1355-8382
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The coat protein gene in RNA 3 of alfalfa mosaic virus (AMV; genus Alfamovirus, family Bromoviridae) is translated from the subgenomic RNA 4. Analysis of the subgenomic promoter (sgp) in minus-strand RNA 3 showed that a sequence of 37 nt upstream of the RNA 4 start site (nt +1) was sufficient for full sgp activity in an in vitro assay with the purified viral RNA-dependent RNA-polymerase (RdRp). The sequence of nt -6 to -29 could be folded into a potential hairpin structure with a loop represented by nt -16, -17, and -18, and a bulge involving nt -23. By introducing mutations that disrupted base pairing and compensatory mutations that restored base pairing, it was shown that base pairing in the top half of the putative stem (between the loop and bulge) was essential for sgp activity, whereas base pairing in the bottom half of the stem was less stringently required. Deletion of the bulged residue A-23 or mutation of this residue into a C strongly reduced sgp activity, but mutation of A-23 into U or G had little effect on sgp activity. Mutation of loop residues A-16 and A-17 affected sgp activity, whereas mutation of U-18 did not. Using RNA templates corresponding to the sgp of brome mosaic virus (BMV; genus Bromovirus, family Bromoviridae) and purified BMV RdRp, evidence was obtained indicating that also in BMV RNA a triloop hairpin structure is required for sgp activity.
[Mh] Termos MeSH primário: Alfamovirus/genética
Bromovirus/genética
Regiões Promotoras Genéticas
RNA Viral/química
RNA Viral/genética
[Mh] Termos MeSH secundário: Pareamento de Bases
Sequência de Bases
Sequência Conservada
Genoma Viral
Mutagênese Sítio-Dirigida
Conformação de Ácido Nucleico
Especificidade da Espécie
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:0006
[Cu] Atualização por classe:140615
[Lr] Data última revisão:
140615
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:000603
[St] Status:MEDLINE


  4 / 7 MEDLINE  
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[PMID]:10416370
[Au] Autor:Jaspars EM
[Ad] Endereço:Institute of Molecular Plant Sciences, Gorlaeus Laboratories, Leiden University, The Netherlands.
[Ti] Título:Genome activation in alfamo- and ilarviruses.
[So] Source:Arch Virol;144(5):843-63, 1999.
[Is] ISSN:0304-8608
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Alfamo- and ilarviruses are characterized by the deficiency of their genomes (three messenger-sense RNAs) to start an infection cycle. The RNAs are in capsids built from a single species of protein of about 24 kD. A few dimers of this coat protein per RNA molecule are sufficient to activate the genome. Since the first description of genome activation [Bol JF, van Vloten-Doting L, Jaspars EMJ (1971) Virology 46: 73-85] three models have been proposed concerning its mechanism: the protection, the replicase and the messenger release hypotheses. The first two models make use of the fact that in these genera of RNA viruses the 3' termini of the RNAs bind the coat protein very strongly. The resulting structure would provide protection against 3'- to 5' exoribonucleases, or would permit correct initiation of minus-strand synthesis, respectively. However, naked inoculated RNAs of alfalfa mosaic virus appear to be quite stable in the cell, and in vitro the coat protein is inhibiting rather than stimulating initiation of minus-strand synthesis. The messenger release hypothesis states that the coat protein is needed for the release of viral messenger RNAs from membranous replication complexes throughout the whole viral replication cycle. This is supported by in vivo and in vitro observations, but as yet a detailed molecular mechanism is difficult to give.
[Mh] Termos MeSH primário: Alfamovirus/genética
Regulação Viral da Expressão Gênica
Genoma Viral
Ilarvirus/genética
[Mh] Termos MeSH secundário: Alfamovirus/fisiologia
Capsídeo/metabolismo
Ilarvirus/fisiologia
RNA Mensageiro/genética
RNA Viral/genética
RNA Viral/metabolismo
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (RNA, Messenger); 0 (RNA, Viral)
[Em] Mês de entrada:9908
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:990723
[St] Status:MEDLINE


  5 / 7 MEDLINE  
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[PMID]:9926399
[Au] Autor:Jaspars EM
[Ad] Endereço:Institute of Molecular Plant Sciences, Gorlaeus Laboratories, Leiden University, The Netherlands. jaspars@chem.leidenuniv.nl
[Ti] Título:A core promoter hairpin is essential for subgenomic RNA synthesis in alfalfa mosaic alfamovirus and is conserved in other Bromoviridae.
[So] Source:Virus Genes;17(3):233-42, 1998.
[Is] ISSN:0920-8569
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The nucleotide sequence immediately in front of the initiation site for subgenomic RNA 4 synthesis on RNA 3 minus strand, which has been proved to function as a core promoter, was inspected for secondary structure in 26 species of the plant virus family Bromoviridae. In 23 cases a stable hairpin could be predicted at a distance of 3 to 8 nucleotides from the initiation site of RNA 4. This hairpin contained several conserved nucleotides that are essential for core promoter activity in brome mosaic virus (R.W. Siegel, S. Adkins and C.C. Kao, Proc. Natl. Acad. Sci. USA 94, 11238-11243, 1997). Phylogenetic evidence and evidence from the effect of artificial mutations reported in the literature (E.A.G. van der Vossen, T. Notenboom and J.F. Bol, Virology 212, 663-672, 1995) indicate that the stem-loop structure is essential for promoter activity in alfalfa mosaic virus and probably in other Bromoviridae. Stability of the hairpin is most pronounced in the genera Alfamovirus and Ilarvirus which display genome activation by coat protein. The hypothesis is put forward that with these viruses the coat protein is needed for the viral RNA polymerase to interact with the core promoter hairpin leading to access for the enzyme to the initiation site of RNA 4.
[Mh] Termos MeSH primário: Alfamovirus/genética
Bromoviridae/genética
Medicago sativa/virologia
Regiões Promotoras Genéticas
RNA Viral/biossíntese
[Mh] Termos MeSH secundário: Alfamovirus/enzimologia
Sequência de Bases
Bromoviridae/enzimologia
Capsídeo/metabolismo
RNA Polimerases Dirigidas por DNA/metabolismo
Mutação
Conformação de Ácido Nucleico
RNA Viral/química
RNA Viral/metabolismo
Homologia de Sequência do Ácido Nucleico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (RNA, Viral); EC 2.7.7.6 (DNA-Directed RNA Polymerases)
[Em] Mês de entrada:9904
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:990202
[St] Status:MEDLINE


  6 / 7 MEDLINE  
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[PMID]:9267012
[Au] Autor:Kasteel DT; van der Wel NN; Jansen KA; Goldbach RW; van Lent JW
[Ad] Endereço:Department of Virology, Agricultural University Wageningen, The Netherlands.
[Ti] Título:Tubule-forming capacity of the movement proteins of alfalfa mosaic virus and brome mosaic virus.
[So] Source:J Gen Virol;78 ( Pt 8):2089-93, 1997 Aug.
[Is] ISSN:0022-1317
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The structural phenotype of the movement proteins (MPs) of two representatives of the Bromoviridae, alfalfa mosaic virus (AMV) and brome mosaic virus (BMV), was studied in protoplasts. Immunofluorescence microscopy showed that the MPs of these viruses, for which there has been no evidence of a tubule-guided mechanism, assemble into long tubular structures at the surface of the infected protoplast. Electron microscopy and immunogold analysis confirmed the presence of both MP and virus particles in the tubules induced by AMV and BMV. The significance of the tubule-forming properties of these viral MPs is discussed.
[Mh] Termos MeSH primário: Alfamovirus/fisiologia
Bromovirus/fisiologia
Proteínas Virais/genética
[Mh] Termos MeSH secundário: Alfamovirus/ultraestrutura
Bromovirus/ultraestrutura
Fabaceae/ultraestrutura
Fabaceae/virologia
Imunofluorescência
Microscopia Eletrônica
Microscopia Imunoeletrônica
Proteínas do Movimento Viral em Plantas
Plantas Medicinais
Protoplastos/ultraestrutura
Protoplastos/virologia
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Viral Movement Proteins); 0 (Viral Proteins)
[Em] Mês de entrada:9709
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:970801
[St] Status:MEDLINE


  7 / 7 MEDLINE  
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[PMID]:9159151
[Au] Autor:Yusibov V; Modelska A; Steplewski K; Agadjanyan M; Weiner D; Hooper DC; Koprowski H
[Ad] Endereço:Biotechnology Foundation Laboratories, Thomas Jefferson University, 1020 Locust Street, Room 346 JAH, Philadelphia, PA 19107, USA. vyusibov@reddi1.uns.tju.edu
[Ti] Título:Antigens produced in plants by infection with chimeric plant viruses immunize against rabies virus and HIV-1.
[So] Source:Proc Natl Acad Sci U S A;94(11):5784-8, 1997 May 27.
[Is] ISSN:0027-8424
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The coat protein (CP) of alfalfa mosaic virus was used as a carrier molecule to express antigenic peptides from rabies virus and HIV. The antigens were separately cloned into the reading frame of alfalfa mosaic virus CP and placed under the control of the subgenomic promoter of tobacco mosaic virus CP in the 30BRz vector. The in vitro transcripts of recombinant virus with sequences encoding the antigenic peptides were synthesized from DNA constructs and used to inoculate tobacco plants. The plant-produced protein (virus particles) was purified and used for immunization of mice. Both antigens elicited specific virus-neutralizing antibodies in immunized mice.
[Mh] Termos MeSH primário: Vacinas contra a AIDS
Alfamovirus/genética
Anticorpos Antivirais/biossíntese
Antígenos Virais/imunologia
Anticorpos Anti-HIV/biossíntese
Antígenos HIV/imunologia
HIV-1/imunologia
Vacinas Antirrábicas
Vírus da Raiva/imunologia
Vacinas Sintéticas
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Anticorpos Antivirais/sangue
Formação de Anticorpos
Antígenos Virais/biossíntese
Linfócitos B/imunologia
Sequência de Bases
Capsídeo/biossíntese
Linhagem Celular
Clonagem Molecular
Primers do DNA
Escherichia coli
Anticorpos Anti-HIV/sangue
Antígenos HIV/biossíntese
Vírus 1 Linfotrópico T Humano/imunologia
Seres Humanos
Rim
Camundongos
Dados de Sequência Molecular
Testes de Neutralização
Regiões Promotoras Genéticas
Proteínas Recombinantes de Fusão/biossíntese
Proteínas Recombinantes de Fusão/imunologia
Linfócitos T/imunologia
Vírus do Mosaico do Tabaco/genética
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (AIDS Vaccines); 0 (Antibodies, Viral); 0 (Antigens, Viral); 0 (DNA Primers); 0 (HIV Antibodies); 0 (HIV Antigens); 0 (Rabies Vaccines); 0 (Recombinant Fusion Proteins); 0 (Vaccines, Synthetic)
[Em] Mês de entrada:9706
[Cu] Atualização por classe:130918
[Lr] Data última revisão:
130918
[Sb] Subgrupo de revista:IM; X
[Da] Data de entrada para processamento:970527
[St] Status:MEDLINE



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