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[PMID]:28632759
[Au] Autor:Kinoti WM; Constable FE; Nancarrow N; Plummer KM; Rodoni B
[Ad] Endereço:Agriculture Victoria, AgriBio, La Trobe University, Melbourne, VIC, Australia.
[Ti] Título:Analysis of intra-host genetic diversity of Prunus necrotic ringspot virus (PNRSV) using amplicon next generation sequencing.
[So] Source:PLoS One;12(6):e0179284, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PCR amplicon next generation sequencing (NGS) analysis offers a broadly applicable and targeted approach to detect populations of both high- or low-frequency virus variants in one or more plant samples. In this study, amplicon NGS was used to explore the diversity of the tripartite genome virus, Prunus necrotic ringspot virus (PNRSV) from 53 PNRSV-infected trees using amplicons from conserved gene regions of each of PNRSV RNA1, RNA2 and RNA3. Sequencing of the amplicons from 53 PNRSV-infected trees revealed differing levels of polymorphism across the three different components of the PNRSV genome with a total number of 5040, 2083 and 5486 sequence variants observed for RNA1, RNA2 and RNA3 respectively. The RNA2 had the lowest diversity of sequences compared to RNA1 and RNA3, reflecting the lack of flexibility tolerated by the replicase gene that is encoded by this RNA component. Distinct PNRSV phylo-groups, consisting of closely related clusters of sequence variants, were observed in each of PNRSV RNA1, RNA2 and RNA3. Most plant samples had a single phylo-group for each RNA component. Haplotype network analysis showed that smaller clusters of PNRSV sequence variants were genetically connected to the largest sequence variant cluster within a phylo-group of each RNA component. Some plant samples had sequence variants occurring in multiple PNRSV phylo-groups in at least one of each RNA and these phylo-groups formed distinct clades that represent PNRSV genetic strains. Variants within the same phylo-group of each Prunus plant sample had ≥97% similarity and phylo-groups within a Prunus plant sample and between samples had less ≤97% similarity. Based on the analysis of diversity, a definition of a PNRSV genetic strain was proposed. The proposed definition was applied to determine the number of PNRSV genetic strains in each of the plant samples and the complexity in defining genetic strains in multipartite genome viruses was explored.
[Mh] Termos MeSH primário: Biomarcadores/metabolismo
Variação Genética/genética
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Ilarvirus/patogenicidade
Doenças das Plantas/virologia
Prunus/virologia
[Mh] Termos MeSH secundário: Ilarvirus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179284


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[PMID]:27283883
[Au] Autor:Thekke Veetil T; Ho T; Moyer C; Whitaker VM; Tzanetakis IE
[Ad] Endereço:Department of Plant Pathology, Division of Agriculture, University of Arkansas System, Fayetteville, AR 72701, United States.
[Ti] Título:Detection of Strawberry necrotic shock virus using conventional and TaqMan(®) quantitative RT-PCR.
[So] Source:J Virol Methods;235:176-81, 2016 Sep.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Graft-indexing of an advanced selection from the University of Florida strawberry breeding program produced virus-like symptoms on Fragaria vesca. However; RT-PCR testing of the material did not detect the presence of any of 16 strawberry virus species or members of virus groups for which strawberries are routinely indexed. Large scale sequencing of the material revealed the presence of an isolate of Strawberry necrotic shock virus. The nucleotide sequence of this isolate from Florida shows a significant number of base changes in the annealing sites of the primers compared to the primers currently in use for the detection of SNSV thereby explaining the most probable reason for the inability to detect the virus in the original screening. RT-PCR and Taqman(®) qPCR assays were developed based on conserved virus sequences identified in this isolate from Florida and other sequences for SNSV currently present in GenBank. The two assays were applied successfully on multiple samples collected from several areas across the United States as well as isolates from around the world. Comparison between the RT-PCR and the qPCR assays revealed that the qPCR assay is at least 100 times more sensitive than conventional PCR.
[Mh] Termos MeSH primário: Fragaria/virologia
Ilarvirus/isolamento & purificação
Doenças das Plantas/virologia
[Mh] Termos MeSH secundário: Primers do DNA
Ilarvirus/classificação
Ilarvirus/genética
Limite de Detecção
Sondas de Oligonucleotídeos
RNA Viral/isolamento & purificação
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (Oligonucleotide Probes); 0 (RNA, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160611
[St] Status:MEDLINE


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[PMID]:26706030
[Au] Autor:Gulati A; Alapati K; Murthy A; Savithri HS; Murthy MR
[Ad] Endereço:Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560012, India.
[Ti] Título:Structural studies on tobacco streak virus coat protein: Insights into the pleomorphic nature of ilarviruses.
[So] Source:J Struct Biol;193(2):95-105, 2016 Feb.
[Is] ISSN:1095-8657
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tobacco streak virus (TSV), the type member of Ilarvirus genus, is a major plant pathogen. TSV purified from infected plants consists of a ss-RNA genome encapsidated in spheroidal particles with diameters of 27, 30 and 33nm constructed from multiple copies of a single species of coat protein (CP) subunits. Apart from protecting the viral genome, CPs of ilarviruses play several key roles in the life cycle of these viruses. Unlike the related bromo and cucumoviruses, ilarvirus particles are labile and pleomorphic, which has posed difficulties in their crystallization and structure determination. In the current study, a truncated TSV-CP was crystallized in two distinct forms and their structures were determined at resolutions of 2.4Å and 2.1Å, respectively. The core of TSV CP was found to possess the canonical ß-barrel jelly roll tertiary structure observed in several other viruses. Dimers of CP with swapped C-terminal arms (C-arm) were observed in both the crystal forms. The C-arm was found to be flexible and is likely to be responsible for the polymorphic and pleomorphic nature of TSV capsids. Consistent with this observation, mutations in the hinge region of the C-arm that reduce the flexibility resulted in the formation of more uniform particles. TSV CP was found to be structurally similar to that of Alfalfa mosaic virus (AMV) accounting for similar mechanism of genome activation in alfamo and ilar viruses. This communication represents the first report on the structure of the CP from an ilarvirus.
[Mh] Termos MeSH primário: Proteínas do Capsídeo/química
Ilarvirus/química
[Mh] Termos MeSH secundário: Vírus do Mosaico da Alfafa/química
Vírus do Mosaico da Alfafa/fisiologia
Proteínas do Capsídeo/genética
Proteínas do Capsídeo/isolamento & purificação
Proteínas do Capsídeo/metabolismo
Simulação por Computador
Cristalografia por Raios X
Ilarvirus/fisiologia
Modelos Moleculares
Conformação Proteica
Multimerização Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Capsid Proteins)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151227
[St] Status:MEDLINE


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[PMID]:26390186
[Au] Autor:Aboughanem-Sabanadzovic N; Tzanetakis IE; Lawrence A; Stephenson RC; Sabanadzovic S
[Ad] Endereço:First author: Institute for Genomics, Biocomputing and Biotechnology, Mississippi State, MS 39762; second author: Department of Plant Pathology, Division of Agriculture, University of Arkansas, Fayetteville 72701; third author: Institute for Imaging and Analytical Technologies, Mississippi State Uni
[Ti] Título:A Novel Ilarvirus Is Associated with Privet Necrotic Ringspot Disease in the Southern United States.
[So] Source:Phytopathology;106(1):87-93, 2016 Jan.
[Is] ISSN:0031-949X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Necrotic ringspot disease (NRSD) is a graft-transmissible disorder of privet (synonym ligustrum), originally reported from Florida and Louisiana more than 50 years ago. In this communication we report an isometric virus isolated from Japanese privet (Ligustrum japonicum) collected in the southern United States displaying symptoms resembling those of NRSD. In mechanical transmission tests, the virus induced systemic infections in several herbaceous hosts. Double-stranded RNA analysis showed a pattern resembling replicative forms of members of the family Bromoviridae. The genome organization along with phylogenetic analyses and serological tests revealed that the virus belongs to subgroup 1 of the genus Ilarvirus. Pairwise comparisons with recognized ilarviruses indicated that the virus is a distinct, and as yet, undescribed member in the taxon, for which we propose the name Privet ringspot virus (PrRSV). Furthermore, the near-perfect association of PrRSV infections with symptoms, and apparent absence of any other virus(es) in studied samples, strongly suggest an important role of this virus in the etiology of NRSD of privet in the southeastern United States.
[Mh] Termos MeSH primário: Ilarvirus/isolamento & purificação
Ligustrum/virologia
Doenças das Plantas/virologia
[Mh] Termos MeSH secundário: Clonagem Molecular
Genoma Viral
Ilarvirus/classificação
Ilarvirus/genética
Filogenia
Folhas de Planta/genética
RNA Viral/genética
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:160115
[Lr] Data última revisão:
160115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150922
[St] Status:MEDLINE
[do] DOI:10.1094/PHYTO-12-14-0387-R


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[PMID]:26234185
[Au] Autor:Galipienso L; Martínez C; Willemsen A; Alfaro-Férnandez A; Ambrosio IF; Davino S; Rubio L
[Ad] Endereço:Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113, Moncada, Valencia, Spain. galipienso_lui@gva.es.
[Ti] Título:Genetic variability and evolutionary analysis of parietaria mottle virus: role of selection and genetic exchange.
[So] Source:Arch Virol;160(10):2611-6, 2015 Oct.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:The genetic variability and evolution of parietaria mottle virus (PMoV) of the genus Ilarvirus was studied by analyzing nucleotide sequences of 2b and CP genes from isolates collected in different countries. Phylogenetic analysis showed that PMoV isolates clustered in different clades: one (clade I) composed of only Italian isolates and three clades (clades II-IV) including the Spanish isolates. The Greek isolate GrT-1 used in this study was in clade IV for the CP phylogenetic tree whereas it formed a separate branch in the 2b phylogenetic tree. The nucleotide sequence diversity of both the 2b and CP genes was low (0.062 ± 0.006 and 0.063 ± 0.006 for 2b and CP, respectively) but higher than those of other ilarviruses. Distribution of synonymous and nonsynonymous substitutions revealed that 2b and CP proteins are under purifying selection, with some positions under diversifying selection. Genetic exchange among Spanish isolates was also detected.
[Mh] Termos MeSH primário: Evolução Molecular
Variação Genética
Ilarvirus/genética
Parietaria/virologia
Doenças das Plantas/virologia
[Mh] Termos MeSH secundário: Evolução Biológica
Proteínas do Capsídeo/genética
Ilarvirus/classificação
Ilarvirus/isolamento & purificação
Dados de Sequência Molecular
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Capsid Proteins)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150926
[Lr] Data última revisão:
150926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150804
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-015-2550-8


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[PMID]:26186968
[Au] Autor:Deguchi A; Tatsuzawa F; Hosokawa M; Doi M; Ohno S
[Ad] Endereço:Laboratory of Vegetable and Ornamental Horticulture, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan.
[Ti] Título:Tobacco streak virus (strain dahlia) suppresses post-transcriptional gene silencing of flavone synthase II in black dahlia cultivars and causes a drastic flower color change.
[So] Source:Planta;242(3):663-75, 2015 Sep.
[Is] ISSN:1432-2048
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:MAIN CONCLUSION: Tobacco streak virus suppressed post-transcriptional gene silencing and caused a flower color change in black dahlias, which supported the role of cyanidin-based anthocyanins for black flower appearance. Black flower color of dahlia (Dahlia variabilis) has been attributed, in part, to the high accumulation of cyanidin-based anthocyanins that occurs when flavone synthesis is reduced because of post-transcriptional gene silencing (PTGS) of flavone synthase II (DvFNS). There are also purple-flowering plants that have emerged from a black cultivar 'Kokucho'. We report that the purple color is not caused by a mutation, as previously thought, but by infection with tobacco streak virus (TSVdahlia), which suppresses the PTGS of DvFNS. When TSVdahlia was eliminated from the purple-flowering 'Kokucho' by leaf primordia-free shoot apical meristem culture, the resulting flowers were black. TSVdahlia-infected purple flowers had lower numbers of siRNAs to DvFNS than black flowers, suggesting that TSVdahlia has a silencing suppressor. The graft inoculation of other black cultivars with TSVdahlia altered their flower color drastically except for 'Fidalgo Blacky', a very deep black cultivar with the highest amount of cyanidin-based anthocyanins. The flowers of all six TSVdahlia-infected cultivars accumulated increased amounts of flavones and reduced amounts of cyanidin-based anthocyanins. 'Fidalgo Blacky' remained black despite the change in pigment accumulation, and the amounts of cyanidin-based anthocyanins in its TSVdahlia-infected plants were still higher than those of other cultivars. We propose that black flower color in dahlia is controlled by two different mechanisms that increase the amount of cyanidin-based anthocyanins: DvFNS PTGS-dependent and -independent mechanisms. If both mechanisms occur simultaneously, the flower color will be blacker than if only a single mechanism is active.
[Mh] Termos MeSH primário: Sistema Enzimático do Citocromo P-450/metabolismo
Dahlia/metabolismo
Flores/metabolismo
Ilarvirus/patogenicidade
Pigmentação/fisiologia
Proteínas de Plantas/metabolismo
[Mh] Termos MeSH secundário: Sistema Enzimático do Citocromo P-450/genética
Dahlia/genética
Dahlia/virologia
Flores/genética
Flores/virologia
Regulação da Expressão Gênica de Plantas
Pigmentação/genética
Proteínas de Plantas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Proteins); 9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.14.- (cytochrome P-450 CYP93B1 (Glycyrrhiza echinata))
[Em] Mês de entrada:1605
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150719
[St] Status:MEDLINE
[do] DOI:10.1007/s00425-015-2365-6


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[PMID]:25907471
[Au] Autor:Dutta M; Ali A; Melcher U
[Ad] Endereço:Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, 74078-3035 USA.
[Ti] Título:Detection, discrimination and discovery of a new Tobacco streak virus strain.
[So] Source:J Virol Methods;221:15-21, 2015 Sep 01.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Soybean plants that exhibited symptoms of virus infection were sampled from different counties of Oklahoma. These plants were tested serologically for 15 viruses known to infect soybean plants. Fifty-seven samples that exhibited typical virus-like symptoms did not test positive for any of the 15 viruses used in a dot-immunobinding assay (DIBA). Four samples were pooled and used for next generation sequencing using the 454-Roche protocol. Sequence and phylogenetic analysis of the sequences obtained revealed infection with a distinct strain of Tobacco streak virus (TSV). TSV was one of the 15 viruses initially tested for using DIBA and had tested negative. TSV belongs to the genus Ilarvirus and has been reported as a causal agent of bud blight in soybean crops in Brazil and the United States. Out of 10 reported primer pairs for TSV reverse transcription-polymerase chain reaction (RT-PCR), only two had the potential, based on sequence similarity, to amplify part of the genome of the distinct strain of TSV found in Oklahoma and only one was actually able to amplify the region. In this study, a new primer pair, specific to all known TSV and capable of amplifying the Oklahoma strain (TSV-OK), was designed from a highly conserved region of coat protein (CP) sequences and end-point PCR and quantitative RT-PCR detection methods were developed and their sensitivity assayed. This is the first report of specific primers designed from this highly conserved region in the CP of TSV for detection of TSV. Twenty-three of the 57 DIBA soybean samples that initially tested negative were retested with the new specific end-point PCR method and found positive for TSV infection.
[Mh] Termos MeSH primário: Ilarvirus/classificação
Ilarvirus/isolamento & purificação
[Mh] Termos MeSH secundário: Análise por Conglomerados
Primers do DNA/genética
Oklahoma
Filogenia
Doenças das Plantas/virologia
Reação em Cadeia da Polimerase/métodos
RNA Viral/genética
Análise de Sequência de DNA
Homologia de Sequência
Feijão de Soja/virologia
Virologia/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (DNA Primers); 0 (RNA, Viral)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150606
[Lr] Data última revisão:
150606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150425
[St] Status:MEDLINE


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[PMID]:25116643
[Au] Autor:Kawamura R; Shimura H; Mochizuki T; Ohki ST; Masuta C
[Ti] Título:Pollen transmission of asparagus virus 2 (AV-2) may facilitate mixed infection by two AV-2 isolates in asparagus plants.
[So] Source:Phytopathology;104(9):1001-6, 2014 Sep.
[Is] ISSN:0031-949X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Asparagus virus 2 (AV-2) is a member of the genus Ilarvirus and thought to induce the asparagus decline syndrome. AV-2 is known to be transmitted by seed, and the possibility of pollen transmission was proposed 25 years ago but not verified. In AV-2 sequence analyses, we have unexpectedly found mixed infection by two distinct AV-2 isolates in two asparagus plants. Because mixed infections by two related viruses are normally prevented by cross protection, we suspected that pollen transmission of AV-2 is involved in mixed infection. Immunohistochemical analyses and in situ hybridization using AV-2-infected tobacco plants revealed that AV-2 was localized in the meristem and associated with pollen grains. To experimentally produce a mixed infection via pollen transmission, two Nicotiana benthamiana plants that were infected with each of two AV-2 isolates were crossed. Derived cleaved-amplified polymorphic sequence analysis identified each AV-2 isolate in the progeny seedlings, suggesting that pollen transmission could indeed result in a mixed infection, at least in N. benthamiana.
[Mh] Termos MeSH primário: Asparagus (Planta)/virologia
Ilarvirus/fisiologia
Doenças das Plantas/virologia
Pólen/virologia
[Mh] Termos MeSH secundário: Proteção Cruzada
Flores/citologia
Flores/virologia
Interações Hospedeiro-Patógeno
Ilarvirus/isolamento & purificação
Imuno-Histoquímica
Hibridização In Situ
Meristema/citologia
Meristema/virologia
Brotos de Planta/citologia
Brotos de Planta/virologia
Pólen/citologia
Polinização
Plântulas/citologia
Plântulas/virologia
Sementes/citologia
Sementes/virologia
Tabaco/citologia
Tabaco/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1410
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140814
[St] Status:MEDLINE
[do] DOI:10.1094/PHYTO-12-13-0348-R


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[PMID]:25110116
[Au] Autor:Zong X; Wang W; Wei H; Wang J; Chen X; Xu L; Zhu D; Tan Y; Liu Q
[Ad] Endereço:Key Laboratory for Fruit Biotechnology Breeding of Shandong Province, Shandong Institute of Pomology, Shandong Academy of Agricultural Sciences, Taian 271000, China.
[Ti] Título:Rapid detection of Prunus necrotic ringspot virus using magnetic nanoparticle-assisted reverse transcription loop-mediated isothermal amplification.
[So] Source:J Virol Methods;208:85-9, 2014 Nov.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Prunus necrotic ringspot virus (PNRSV) has seriously reduced the yield of Prunus species worldwide. In this study, a highly efficient and specific two-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) was developed to detect PNRSV. Total RNA was extracted from sweet cherry leaf samples using a commercial kit based on a magnetic nanoparticle technique. Transcripts were used as the templates for the assay. The results of this assay can be detected using agarose gel electrophoresis or by assessing in-tube fluorescence after adding SYBR Green I. The assay is highly specific for PNRSV, and it is more sensitive than reverse-transcription polymerase chain reaction (RT-PCR). Restriction enzyme digestion verified further the reliability of this RT-LAMP assay. To our knowledge, this is the first report of the application of RT-LAMP to PNRSV detection in Prunus species.
[Mh] Termos MeSH primário: Ilarvirus/isolamento & purificação
Nanopartículas
Técnicas de Amplificação de Ácido Nucleico/métodos
[Mh] Termos MeSH secundário: Eletroforese em Gel de Ágar
Fluorescência
Ilarvirus/genética
Magnetismo
Dados de Sequência Molecular
Compostos Orgânicos/metabolismo
Doenças das Plantas/virologia
Prunus/virologia
RNA Viral/genética
Transcrição Reversa
Sensibilidade e Especificidade
Análise de Sequência de DNA
Coloração e Rotulagem
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Organic Chemicals); 0 (RNA, Viral); 163795-75-3 (SYBR Green I)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140812
[St] Status:MEDLINE


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[PMID]:24583367
[Au] Autor:Martínez C; Coll-Bonfill N; Aramburu J; Pallás V; Aparicio F; Galipienso L
[Ad] Endereço:Institut de Recerca i Tecnología Agroalimentaries (IRTA), Ctra. de Cabrils s/n Cabrils, 08348 Barcelona, Spain. Electronic address: caromar88@gmail.com.
[Ti] Título:Two basic (hydrophilic) regions in the movement protein of Parietaria mottle virus have RNA binding activity and are required for cell-to-cell transport.
[So] Source:Virus Res;184:54-61, 2014 May 12.
[Is] ISSN:1872-7492
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The movement protein (MP) of parietaria mottle virus (PMoV) is required for virus cell-to-cell movement. Bioinformatics analysis identified two hydrophilic non-contiguous regions (R1 and R2) rich in the basic amino acids lysine and arginine and with the predicted secondary structure of an α-helix. Different approaches were used to determine the implication of the R1 and R2 regions in RNA binding, plasmodesmata (PD) targeting and cell-to-cell movement. EMSA (Electrophoretic Mobility Shift Assay) showed that both regions have RNA-binding activity whereas that mutational analysis reported that either deletion of any of these regions, or loss of the basic amino acids, interfered with the viral intercellular movement. Subcellular localization studies showed that PMoV MP locates at PD. Mutants designed to impeded cell-to-cell movement failed to accumulate at PD indicating that basic residues in both R1 and R2 are critical for binding the MP at PD.
[Mh] Termos MeSH primário: Ilarvirus/fisiologia
Proteínas do Movimento Viral em Plantas/metabolismo
Proteínas de Ligação a RNA/metabolismo
Internalização do Vírus
Liberação de Vírus
[Mh] Termos MeSH secundário: Arginina/química
Arginina/genética
Biologia Computacional
Análise Mutacional de DNA
Ensaio de Desvio de Mobilidade Eletroforética
Lisina/química
Lisina/genética
Proteínas do Movimento Viral em Plantas/química
Proteínas do Movimento Viral em Plantas/genética
Ligação Proteica
Conformação Proteica
Estrutura Terciária de Proteína
RNA/metabolismo
Proteínas de Ligação a RNA/química
Proteínas de Ligação a RNA/genética
Deleção de Sequência
Eletricidade Estática
Tabaco/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Viral Movement Proteins); 0 (RNA-Binding Proteins); 63231-63-0 (RNA); 94ZLA3W45F (Arginine); K3Z4F929H6 (Lysine)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:140415
[Lr] Data última revisão:
140415
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140304
[St] Status:MEDLINE



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