[PMID]: | 27923589 |
[Au] Autor: | Bruisson S; Lebel S; Walter B; Prevotat L; Seddas S; Schellenbaum P |
[Ad] Endereço: | Université de Haute-Alsace, Laboratoire Vigne Biotechnologies & Environnement, 33 rue de Herrlisheim, 68100, Colmar, France; SEDIAG SAS Company, Technopôle Agro-Environnement, RD 31, 21110 Bretenière, France. |
[Ti] Título: | Comparative detection of a large population of grapevine viruses by TaqMan RT-qPCR and ELISA. |
[So] Source: | J Virol Methods;240:73-77, 2017 Feb. |
[Is] ISSN: | 1879-0984 |
[Cp] País de publicação: | Netherlands |
[La] Idioma: | eng |
[Ab] Resumo: | Grapevine (Vitis spp.) can be infected by numerous viruses that are often widespread and of great economic importance. Reliable detection methods are necessary for sanitary selection which is the only way to partly control grapevine virus diseases. Biological indexing and ELISA are currently the standard methods for screening propagation material, and PCR-methods are becoming increasingly popular. Due to the diversity of virus isolates, it is essential to verify that the tests allow the detection of the largest possible virus populations. We developed three quadruplex TaqMan RT-qPCR assays for detecting nine different viruses that cause considerable damage in many vineyards world-wide. Each assay is designed to detect three viruses and the grapevine Actin as an internal control. A large population of grapevines from diverse cultivars and geographic location was tested for the presence of nine viruses: Arabis mosaic virus (ArMV), Grapevine fleck virus (GFkV), Grapevine fanleaf virus (GFLV), Grapevine leafroll-associated viruses (GLRaV-1, -2, -3), Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine virus A (GVA), and Grapevine virus B (GVB). In general, identical results were obtained with multiplex TaqMan RT-qPCR and ELISA although, in some cases, viruses could be detected by only one of the two techniques. |
[Mh] Termos MeSH primário: |
Closteroviridae/isolamento & purificação Ensaio de Imunoadsorção Enzimática Flexiviridae/isolamento & purificação Nepovirus/isolamento & purificação Reação em Cadeia da Polimerase em Tempo Real Tymoviridae/isolamento & purificação Vitis/virologia
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[Mh] Termos MeSH secundário: |
Closteroviridae/genética Closteroviridae/imunologia Primers do DNA DNA Complementar Flexiviridae/genética Flexiviridae/imunologia Nepovirus/genética Nepovirus/imunologia Doenças das Plantas/virologia RNA Viral/isolamento & purificação Tymoviridae/genética Tymoviridae/imunologia
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[Pt] Tipo de publicação: | COMPARATIVE STUDY; JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (DNA Primers); 0 (DNA, Complementary); 0 (RNA, Viral) |
[Em] Mês de entrada: | 1710 |
[Cu] Atualização por classe: | 171013 |
[Lr] Data última revisão:
| 171013 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 161208 |
[St] Status: | MEDLINE |
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