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[PMID]: 28710957 [Au] Autor: Prator CA; Kashiwagi CM; Voncina D; Almeida RPP [Ad] Endereço: Department of Environmental Science, Policy, and Management, University of California, Berkeley, CA 94720, USA. [Ti] Título: Infection and Colonization of Nicotiana benthamiana by Grapevine leafroll-associated virus 3. [So] Source: Virology;510:60-66, 2017 Oct. [Is] ISSN: 1096-0341 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Grapevine leafroll disease is an increasing problem in all grape-growing regions of the world. The most widespread agent of the disease, Grapevine leafroll-associated virus 3 (GLRaV-3), has never been shown to infect species outside of the genus Vitis. Virus transmission to several plant species used as model systems was tested using the vine mealybug, Planococcus ficus. We show that GLRaV-3 is able to infect Nicotiana benthamiana. Working with GLRaV-3 infected N. benthamiana revealed distinct advantages in comparison with its natural host Vitis vinifera, yielding both higher viral protein and virion concentrations in western blot and transmission electron microscopy observations, respectively. Immunogold labelling of thin sections through N. benthamiana petioles revealed filamentous particles in the phloem cells of GLRaV-3 positive plants. Comparison of assembled whole genomes from GLRaV-3 infected V. vinifera vs. N. benthamiana revealed substitutions in the 5' UTR. These results open new avenues and opportunities for GLRaV-3 research. [Mh] Termos MeSH primário: Closteroviridae/crescimento & desenvolvimento Especificidade de Hospedeiro Tabaco/virologia [Mh] Termos MeSH secundário: Animais Western Blotting Closteroviridae/fisiologia Hemípteros/virologia Insetos Vetores Microscopia Eletrônica de Transmissão Análise de Sequência de DNA Vitis/virologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170829 [Lr] Data última revisão: 170829 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170716 [St] Status: MEDLINE

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[PMID]: 28025711 [Au] Autor: Bester R; Burger JT; Maree HJ [Ad] Endereço: Department of Genetics, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa. [Ti] Título: Differential expression of miRNAs and associated gene targets in grapevine leafroll-associated virus 3-infected plants. [So] Source: Arch Virol;162(4):987-996, 2017 Apr. [Is] ISSN: 1432-8798 [Cp] País de publicação: Austria [La] Idioma: eng [Ab] Resumo: MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs (sRNA) that play an essential role in the regulation of target mRNAs expressed during plant development and in response to stress. MicroRNA expression profiling has helped to identify miRNAs that regulate a range of processes, including the plant's defence response to pathogens. In this study, differential miRNA expression in own-rooted Vitis vinifera cv. Cabernet Sauvignon plants infected with grapevine leafroll-associated virus 3 was investigated with microarrays and next-generation sequencing (NGS) of sRNA and mRNA. These high-throughput approaches identified several differentially expressed miRNAs. Four miRNAs, identified by both approaches, were validated by stemloop RT-PCRs. Three of the predicted targets of the differentially expressed miRNAs were also differentially expressed in the transcriptome data of infected plants, and were validated by RT-qPCR. Identification of these miRNAs and their targets can lead to a better understanding of host-pathogen interactions involved in grapevine leafroll disease and the identification of possible targets for virus resistance. [Mh] Termos MeSH primário: Closteroviridae/fisiologia Regulação da Expressão Gênica de Plantas MicroRNAs/metabolismo Doenças das Plantas/genética Proteínas de Plantas/genética RNA de Plantas/metabolismo Vitis/metabolismo [Mh] Termos MeSH secundário: Closteroviridae/genética Closteroviridae/isolamento & purificação Interações Hospedeiro-Patógeno MicroRNAs/genética Doenças das Plantas/virologia Proteínas de Plantas/metabolismo RNA de Plantas/genética Vitis/genética Vitis/virologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (MicroRNAs); 0 (Plant Proteins); 0 (RNA, Plant) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170315 [Lr] Data última revisão: 170315 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161228 [St] Status: MEDLINE [do] DOI: 10.1007/s00705-016-3197-9

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[PMID]: 27938242 [Au] Autor: Arnold K; Golino DA; McRoberts N [Ad] Endereço: All authors: Department of Plant Pathology, and second author: Foundation Plant Services, University of California, Davis 95616. [Ti] Título: A Synoptic Analysis of the Temporal and Spatial Aspects of Grapevine Leafroll Disease in a Historic Napa Vineyard and Experimental Vine Blocks. [So] Source: Phytopathology;107(4):418-426, 2017 04. [Is] ISSN: 0031-949X [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Five Grapevine leafroll-associated virus 3 (GLRaV-3) epidemics were analyzed utilizing a standardized approach to robustly characterize the temporal and spatial parameters. Published data included in the analysis are from Spain, New Zealand, and Napa Valley, CA together with new data from a historic vineyard in Napa Valley, CA. Linear regression analyses of logit-transformed incidence data indicated a maximum average increase of 11% per year in disease incidence, with considerable variation among locations. Spatial analyses, including distribution fitting, examination of the effective sample size, and evaluation of the parameters of the binary power law fitted to variance data for disease incidence, indicated a high degree of consistency among the data sets. In all cases, except at very low disease incidence, a high degree of spatial aggregation was noted, with evidence that the degree of aggregation varied as a function of mean disease incidence. The polyetic dynamics of disease follow a logistic-like pattern over multiple seasons, consistent with limitation by inoculum availability (infected vines) at low incidence and limitation by disease-free vines at high incidence. [Mh] Termos MeSH primário: Closteroviridae/isolamento & purificação Doenças das Plantas/virologia Vitis/virologia [Mh] Termos MeSH secundário: California Closteroviridae/genética Nova Zelândia Espanha Análise Espaço-Temporal [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170720 [Lr] Data última revisão: 170720 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161213 [St] Status: MEDLINE [do] DOI: 10.1094/PHYTO-06-16-0235-R

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[PMID]: 27923589 [Au] Autor: Bruisson S; Lebel S; Walter B; Prevotat L; Seddas S; Schellenbaum P [Ad] Endereço: Université de Haute-Alsace, Laboratoire Vigne Biotechnologies & Environnement, 33 rue de Herrlisheim, 68100, Colmar, France; SEDIAG SAS Company, Technopôle Agro-Environnement, RD 31, 21110 Bretenière, France. [Ti] Título: Comparative detection of a large population of grapevine viruses by TaqMan RT-qPCR and ELISA. [So] Source: J Virol Methods;240:73-77, 2017 Feb. [Is] ISSN: 1879-0984 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Grapevine (Vitis spp.) can be infected by numerous viruses that are often widespread and of great economic importance. Reliable detection methods are necessary for sanitary selection which is the only way to partly control grapevine virus diseases. Biological indexing and ELISA are currently the standard methods for screening propagation material, and PCR-methods are becoming increasingly popular. Due to the diversity of virus isolates, it is essential to verify that the tests allow the detection of the largest possible virus populations. We developed three quadruplex TaqMan RT-qPCR assays for detecting nine different viruses that cause considerable damage in many vineyards world-wide. Each assay is designed to detect three viruses and the grapevine Actin as an internal control. A large population of grapevines from diverse cultivars and geographic location was tested for the presence of nine viruses: Arabis mosaic virus (ArMV), Grapevine fleck virus (GFkV), Grapevine fanleaf virus (GFLV), Grapevine leafroll-associated viruses (GLRaV-1, -2, -3), Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine virus A (GVA), and Grapevine virus B (GVB). In general, identical results were obtained with multiplex TaqMan RT-qPCR and ELISA although, in some cases, viruses could be detected by only one of the two techniques. [Mh] Termos MeSH primário: Closteroviridae/isolamento & purificação Ensaio de Imunoadsorção Enzimática Flexiviridae/isolamento & purificação Nepovirus/isolamento & purificação Reação em Cadeia da Polimerase em Tempo Real Tymoviridae/isolamento & purificação Vitis/virologia [Mh] Termos MeSH secundário: Closteroviridae/genética Closteroviridae/imunologia Primers do DNA DNA Complementar Flexiviridae/genética Flexiviridae/imunologia Nepovirus/genética Nepovirus/imunologia Doenças das Plantas/virologia RNA Viral/isolamento & purificação Tymoviridae/genética Tymoviridae/imunologia [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA Primers); 0 (DNA, Complementary); 0 (RNA, Viral) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171013 [Lr] Data última revisão: 171013 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161208 [St] Status: MEDLINE

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[PMID]: 27391019 [Au] Autor: Weinheimer I; Haikonen T; Ala-Poikela M; Moser M; Streng J; Rajamäki ML; Valkonen JP [Ad] Endereço: Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland. [Ti] Título: Viral RNase3 Co-Localizes and Interacts with the Antiviral Defense Protein SGS3 in Plant Cells. [So] Source: PLoS One;11(7):e0159080, 2016. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Sweet potato chlorotic stunt virus (SPCSV; family Closteroviridae) encodes a Class 1 RNase III endoribonuclease (RNase3) that suppresses post-transcriptional RNA interference (RNAi) and eliminates antiviral defense in sweetpotato plants (Ipomoea batatas). For RNAi suppression, RNase3 cleaves double-stranded small interfering RNAs (ds-siRNA) and long dsRNA to fragments that are too short to be utilized in RNAi. However, RNase3 can suppress only RNAi induced by sense RNA. Sense-mediated RNAi involves host suppressor of gene silencing 3 (SGS3) and RNA-dependent RNA polymerase 6 (RDR6). In this study, subcellular localization and host interactions of RNase3 were studied in plant cells. RNase3 was found to interact with SGS3 of sweetpotato and Arabidopsis thaliana when expressed in leaves, and it localized to SGS3/RDR6 bodies in the cytoplasm of leaf cells and protoplasts. RNase3 was also detected in the nucleus. Co-expression of RNase3 and SGS3 in leaf tissue enhanced the suppression of RNAi, as compared with expression of RNase3 alone. These results suggest additional mechanisms needed for efficient RNase3-mediated suppression of RNAi and provide new information about the subcellular context and phase of the RNAi pathway in which RNase3 realizes RNAi suppression. [Mh] Termos MeSH primário: Closteroviridae/enzimologia Proteína Catiônica de Eosinófilo/metabolismo Células Vegetais/metabolismo Células Vegetais/virologia [Mh] Termos MeSH secundário: Citoplasma/genética Citoplasma/metabolismo Doenças das Plantas/virologia Folhas de Planta/genética Folhas de Planta/metabolismo Proteínas de Plantas/genética Proteínas de Plantas/metabolismo Interferência de RNA RNA Interferente Pequeno/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Plant Proteins); 0 (RNA, Small Interfering); EC 3.1.27.- (Eosinophil Cationic Protein) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170818 [Lr] Data última revisão: 170818 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160709 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0159080

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[PMID]: 27329635 [Au] Autor: Wistrom CM; Blaisdell GK; Wunderlich LR; Almeida RP; Daane KM [Ad] Endereço: Department of Environmental Science, Policy and Management, University of California, Berkeley, CA 94720-3114 (cwistrom@berkeley.edu; blaisdell@berkeley.edu; rodrigoalmeida@berkeley.edu; kdaane@ucanr.edu). [Ti] Título: Ferrisia gilli (Hemiptera: Pseudococcidae) Transmits Grapevine Leafroll-Associated Viruses. [So] Source: J Econ Entomol;109(4):1519-23, 2016 Aug. [Is] ISSN: 1938-291X [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Several mealybug species are vectors of grapevine leafroll-associated viruses (GLRaV), which cause the economically important grapevine leafroll disease in grape-producing regions worldwide. The mealybug Ferrisia gilli Gullan is a new pest of grapevines in El Dorado County, located in the Sierra Foothill wine-growing region of California. GLRaV species 1, 2, 3, and 4LV have been detected in vineyards with symptomatic vines in the Sierra Foothills. We conducted controlled virus acquisition and transmission experiments using source vine accessions infected with different combinations of GLRaV. We determined that F. gilli acquired GLRaV 1, 2, 3, and 4LV, and transmitted GLRaV-3 and GLRaV-4LV to uninfected recipient vines. Like numerous other mealybug species, in addition to causing direct damage to vines, F. gilli poses a threat to the grape industry as a vector of economically damaging viruses. [Mh] Termos MeSH primário: Closteroviridae/fisiologia Hemípteros/fisiologia Doenças das Plantas/virologia Vitis/virologia [Mh] Termos MeSH secundário: Animais California Closteroviridae/classificação Hemípteros/crescimento & desenvolvimento Hemípteros/virologia Ninfa/crescimento & desenvolvimento Ninfa/fisiologia Ninfa/virologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170823 [Lr] Data última revisão: 170823 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160623 [St] Status: MEDLINE [do] DOI: 10.1093/jee/tow124

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[PMID]: 27329628 [Au] Autor: Bertin S; Cavalieri V; Gribaudo I; Sacco D; Marzachì C; Bosco D [Ad] Endereço: DISAFA, Università degli Studi di Torino, Largo Paolo Braccini 2, 10095 Grugliasco, Italy (sabrina.bertin@crea.gov.it; vicavalieri78@gmail.com; dario.sacco@unito.it; domenico.bosco@unito.it), CREA - PAV, Via C.G. Bertero 22, 00156 Roma, Italy. [Ti] Título: Transmission of Grapevine virus A and Grapevine leafroll-associated virus 1 and 3 by Heliococcus bohemicus (Hemiptera: Pseudococcidae) Nymphs From Plants With Mixed Infections. [So] Source: J Econ Entomol;109(4):1504-11, 2016 Aug. [Is] ISSN: 1938-291X [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Mealybugs (Hemiptera: Pseudococcidae) represent a serious threat for viticulture as vectors of phloem-restricted viruses associated with the grapevine rugose wood and leafroll diseases. Heliococcus bohemicus (Sulc) is known to be involved in the spread of these two viral diseases, being a vector of the Grapevine virus A (GVA) and the Grapevine leafroll-associated virus 1 and 3 (GLRaV-1 and GLRaV-3). This study investigated the acquisition and transmission efficiency of H. bohemicus fed on mixed-infected plants. Nymphs were field-collected onto GVA, GLRaV-1, and GLRaV-3 multiple-infected grapevines in two vineyards in North-Western Italy, and were used in transmission experiments under controlled conditions. Even if most of the collected nymphs were positive to at least one virus, transmission occurred only to a low number of test grapevines. The transmission frequency of GLRaV-3 was the highest, whereas GVA was transmitted to few test plants. The transmission of multiple viruses occurred at low rates, and nymphs that acquired all the three viruses then failed to transmit them together. Statistical analyses showed that the three viruses were independently acquired and transmitted by H. bohemicus and neither synergistic nor antagonistic interactions occurred among them. GVA and GLRaVs transmission efficiencies by H. bohemicus were lower than those reported for other mealybug vectors. This finding is consistent with the slow spread of leafroll and rugose wood diseases observed in Northern Italy, where H. bohemicus is the predominant vector species. [Mh] Termos MeSH primário: Closteroviridae/fisiologia Flexiviridae/fisiologia Hemípteros/fisiologia Doenças das Plantas/virologia Vitis/virologia [Mh] Termos MeSH secundário: Animais Hemípteros/crescimento & desenvolvimento Hemípteros/virologia Itália Ninfa/crescimento & desenvolvimento Ninfa/fisiologia Ninfa/virologia Análise de Sequência de RNA [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170823 [Lr] Data última revisão: 170823 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160623 [St] Status: MEDLINE [do] DOI: 10.1093/jee/tow120

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[PMID]: 27265467 [Au] Autor: Strukelj M; Plesko IM; Urek G [Ti] Título: Molecular characterization of a grapevine leafroll-associated virus 4 from Slovenian vineyards. [So] Source: Acta Virol;60(2):174-80, 2016 Jun. [Is] ISSN: 0001-723X [Cp] País de publicação: Slovakia [La] Idioma: eng [Ab] Resumo: During a survey conducted in vineyards in Slovenia, variety of grapevine leafroll disease symptoms were observed. Mixed infection with grapevine leafroll-associated viruses 3 and 4 (GLRaV-3, -4) in two grapevines from a vineyard in south-western part of Slovenia was confirmed by DAS-ELISA in 2010. The 3'final 1769 nucleotides of the Slovenian GLRaV-4 isolate were assembled from amplicons obtained by IC RT-PCR. The complete coat protein (CP) and p23 gene sequences were compared with other GLRaV-4 sequences from GenBank. Results showed that CP and p23 amino acid sequences of Slovenian variant (055-SI) are 88% and 85%, respectively, identical to corresponding genes of reference sequence GLRaV-4 LR106 (GenBank Acc. No. FJ467503). Phylogenetic analyses show that Slovenian variant clusters together with other corresponding strains of GLRaV-4. The sequencing results show great variability of the N-terminal part of the CP sequence indicating that this part of the genome is not suitable for molecular detection of the virus. To our knowledge this is also the first report of GLRaV-4 in Slovenian vineyards. [Mh] Termos MeSH primário: Closteroviridae/genética Doenças das Plantas/virologia Vitis/virologia [Mh] Termos MeSH secundário: Closteroviridae/química Closteroviridae/classificação Closteroviridae/isolamento & purificação Genoma Viral Dados de Sequência Molecular Filogenia Homologia de Sequência de Aminoácidos Proteínas Virais/química Proteínas Virais/genética [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Viral Proteins) [Em] Mês de entrada: 1609 [Cu] Atualização por classe: 160606 [Lr] Data última revisão: 160606 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160607 [St] Status: MEDLINE

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[PMID]: 27246908 [Au] Autor: Poojari S; Alabi OJ; Okubara PA; Naidu RA [Ad] Endereço: Department of Plant Pathology, Washington State University, Irrigated Agriculture Research and Extension Center, Prosser, WA, USA; Agriculture and Agri-Food Canada, Summerland Research and Development Centre, Summerland, British Columbia V0H 1Z0, Canada. [Ti] Título: SYBR(®) Green-based real-time quantitative reverse-transcription PCR for detection and discrimination of grapevine viruses. [So] Source: J Virol Methods;235:112-8, 2016 Sep. [Is] ISSN: 1879-0984 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: A SYBR(®) Green-based real-time quantitative reverse transcription PCR (qRT-PCR) assay in combination with melt-curve analysis (MCA) was optimized for the detection of nine grapevine viruses. The detection limits for simplex qRT-PCR for all nine grapevine viruses were estimated to be in the range of 214-1112 copies of the virus genome. Amplicons with melting temperatures (Tm) separated by at least 2°C in the MCA could differentiate two viruses in the same reaction. Therefore, eight of the nine viruses could be co-diagnosed in five different combinations of duplex assays. Of 305 grape leaf samples from the field or greenhouse, 162 were positive for at least one of the nine grapevine viruses using the duplex qRT-PCR assays. In contrast, only 127 samples were positive using endpoint RT-PCR and PCR assays, indicating the enhanced sensitivity of duplex real-time PCR. In addition, the duplex qRT-PCR assays were be used to detect Grapevine leafroll associated virus 3 (GLRaV-3) in its vector, the grape mealybug (Pseudococcus maritimus Ehrhorn), and Grapevine red blotch-associated virus (GRBaV) in Virginia creeper leafhopper (Erythroneura ziczac Walsh). The simplex and duplex real-time PCR assays developed in this study can be used to examine transmission of co-occruing viruses by insect vectors as well as for rapid and sensitive detection of viruses in infected grapevines. [Mh] Termos MeSH primário: Closteroviridae/isolamento & purificação Compostos Orgânicos Doenças das Plantas/virologia Folhas de Planta/virologia Reação em Cadeia da Polimerase em Tempo Real/métodos Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos [Mh] Termos MeSH secundário: Animais Closteroviridae/genética Genoma Viral Hemípteros/virologia Limite de Detecção Compostos Orgânicos/química RNA Viral/genética RNA Viral/isolamento & purificação Sensibilidade e Especificidade Temperatura de Transição Vitis/virologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Organic Chemicals); 0 (RNA, Viral); 163795-75-3 (SYBR Green I) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171013 [Lr] Data última revisão: 171013 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160602 [St] Status: MEDLINE

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[PMID]: 27153513 [Au] Autor: El Aou-Ouad H; Montero R; Medrano H; Bota J [Ad] Endereço: Grup de Recerca en Biologia de les Plantes en Condicions Mediterrànies, Departament de Biologia, Universitat de les Illes Balears, Carretera de Valldemossa, km 7.5, 07122, Palma de Mallorca, Balears, Spain. [Ti] Título: Interactive effects of grapevine leafroll-associated virus 3 (GLRaV-3) and water stress on the physiology of Vitis vinifera L. cv. Malvasia de Banyalbufar and Giro-Ros. [So] Source: J Plant Physiol;196-197:106-15, 2016 Jun 01. [Is] ISSN: 1618-1328 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: Among several biotic and abiotic stress combinations, interaction between drought and pathogen is one of the most studied combinations in some crops but still not in grapevine. In the present work, we focused on the interaction effects of biotic (GLRaV-3) and abiotic (drought) stresses on grapevine photosynthetic metabolism on two cultivars (cvs. 'Malvasia de Banyalbufar and Giro-Ros'). Non-infected and GLRaV-3 infected potted plants were compared under water stress conditions (WS) and well-watered (WW) conditions. Under WW condition, the results showed that photosynthesis (AN) in both cultivars was decreased by the presence of GLRaV-3. The stomatal conductance (gs) was the main factor for decreasing AN in Malvasia, meanwhile reductions in Giro-Ros were closely related to decreases in gm. The observed differences in gm between both cultivars might result from variation in their leaf anatomical, Giro-Ros having higher values of gm and leaf porosity (in all treatments). Moderate water deficit resulted in a closure of stomata and a decrease in gm accompanied by a decrease in AN in both cultivars. The maximum velocity of carboxylation (Vcmax) and electron transport rate (Jmax) were also reduced under water stress. Moreover, the combined stress resulted in a reduction of most physiological parameters compared to healthy irrigated plants. However, no considerable differences were found between non-infected and virus infected (GLRaV-3) plants under water stress. Most of the results could be explained by the difference of virus concentration between cultivars and treatments. [Mh] Termos MeSH primário: Closteroviridae/fisiologia Secas Fotossíntese Vitis/fisiologia [Mh] Termos MeSH secundário: Folhas de Planta/fisiologia Folhas de Planta/virologia Espanha Vitis/genética Vitis/virologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170310 [Lr] Data última revisão: 170310 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160507 [St] Status: MEDLINE

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