Base de dados : MEDLINE
Pesquisa : B04.715.110.150 [Categoria DeCS]
Referências encontradas : 254 [refinar]
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[PMID]:28888112
[Au] Autor:Harper SJ; Cowell SJ; Dawson WO
[Ad] Endereço:Department of Plant Pathology, Washington State University, Prosser, WA 99350, USA. Electronic address: scott.harper@wsu.edu.
[Ti] Título:Isolate fitness and tissue-tropism determine superinfection success.
[So] Source:Virology;511:222-228, 2017 Nov.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The mechanism of cross-protection, the deliberate infection of plants with a "mild" virus isolate to protect against "severe" isolates, has long been a topic of debate. In our model system, Citrus tristeza virus (CTV), this appears to be genotype-specific superinfection-exclusion, suggesting a simple recipe for cross-protection. However, this concept failed in field trials, which led us to examine the process of superinfection-exclusion more closely. We found that exclusion relies on the relative fitness of the primary versus the challenge isolates, and the host infected, and that significant differences in superinfection success could occur between isolates that differ by as few as 3 nucleotides. Furthermore, we found that exclusion was not uniform throughout the plant, but was tissue-specific. These data suggest that cross-protection is not a simple like-for-like process but a complex interaction between the primary and challenge isolates and the host.
[Mh] Termos MeSH primário: Citrus/virologia
Closterovirus/fisiologia
Doenças das Plantas/virologia
Superinfecção/virologia
Interferência Viral
Tropismo Viral
[Mh] Termos MeSH secundário: Interações Hospedeiro-Patógeno
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170910
[St] Status:MEDLINE


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[PMID]:28027478
[Au] Autor:Gushchin VA; Karlin DG; Makhotenko AV; Khromov AV; Erokhina TN; Solovyev AG; Morozov SY; Agranovsky AA
[Ad] Endereço:Faculty of Biology, Moscow State University, Moscow 119991, Russia; N.F. Gamaleya Federal Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Russia.
[Ti] Título:A conserved region in the Closterovirus 1a polyprotein drives extensive remodeling of endoplasmic reticulum membranes and induces motile globules in Nicotiana benthamiana cells.
[So] Source:Virology;502:106-113, 2017 Feb.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In infected plant cells, closterovirus replicative polyproteins 1a and 1ab drive membrane remodeling and formation of multivesicular replication platforms. Polyprotein 1a contains a variable Central Region (CR) between the methyltransferase and helicase domains. In a previous study, we have found that transient expression of the Beet yellows virus CR-2 segment (aa 1305-1494) in Nicotiana benthamiana induces the formation of ~1µm mobile globules originating from the ER membranes. In the present study, sequence analysis has shown that a part of the CR named the "Zemlya region" (overlapping the CR-2), is conserved in all members of the Closterovirus genus and contains a predicted amphipathic helix (aa 1368-1385). By deletion analysis, the CR-2 region responsible for the induction of 1-µm globules has been mapped to aa 1368-1432. We suggest that the conserved membrane-modifying region of the BYV 1a may be involved in the biogenesis of closterovirus replication platforms.
[Mh] Termos MeSH primário: Closterovirus/genética
Retículo Endoplasmático/virologia
Doenças das Plantas/virologia
Poliproteínas/química
Poliproteínas/metabolismo
Tabaco/virologia
Proteínas Virais/química
Proteínas Virais/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Closterovirus/química
Closterovirus/metabolismo
Sequência Conservada
Retículo Endoplasmático/metabolismo
Dados de Sequência Molecular
Poliproteínas/genética
Alinhamento de Sequência
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polyproteins); 0 (Viral Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170622
[Lr] Data última revisão:
170622
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161228
[St] Status:MEDLINE


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[PMID]:27743959
[Au] Autor:Bergua M; Kang SH; Folimonova SY
[Ad] Endereço:University of Florida, Department of Plant Pathology, 2550 Hull Road, Gainesville, FL 32611, USA.
[Ti] Título:Understanding superinfection exclusion by complex populations of Citrus tristeza virus.
[So] Source:Virology;499:331-339, 2016 Dec.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Superinfection exclusion (SIE) is a phenomenon in which a primary viral infection restricts a secondary infection with the same or closely related virus. Previously we showed that SIE by Citrus tristeza virus (CTV) occurs only between isolates of the same virus genotype. This work, however, was done using single genotype-containing isolates, while most field citrus trees harbor complex populations composed of different virus genotypes. Here we examined SIE in plants simultaneously infected with several CTV genotypes. The experiments showed that exclusion of a secondary infection by a CTV variant was triggered by the presence of another variant of the same genotype in the primary population, even under the conditions of its low-level accumulation, and was not affected by co-occurrence of additional heterologous genotypes. The same rule appeared to be in effect when SIE by mixed populations was tested in a series of different citrus varieties.
[Mh] Termos MeSH primário: Citrus/virologia
Closterovirus/fisiologia
Doenças das Plantas/virologia
Superinfecção/prevenção & controle
[Mh] Termos MeSH secundário: Closterovirus/genética
Genótipo
RNA Viral/genética
RNA Viral/metabolismo
Superinfecção/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170908
[Lr] Data última revisão:
170908
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161017
[St] Status:MEDLINE


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[PMID]:27659675
[Au] Autor:Cowell SJ; Harper SJ; Dawson WO
[Ad] Endereço:Citrus Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, 700 Experiment Station Road, Lake Alfred, FL, 33850, USA. sjcowell@ufl.edu.
[Ti] Título:Some like it hot: citrus tristeza virus strains react differently to elevated temperature.
[So] Source:Arch Virol;161(12):3567-3570, 2016 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Viruses often infect plants as a mixed population. The dynamics of viral populations dictate the success of the infection, yet there is little understanding of the factors that influence them. It is known that temperature can affect individual viruses; could it also affect a virus population? In order to study this, we observed citrus tristeza virus (CTV) populations in different hosts under winter and summer conditions (25 versus 36 °C). We found that only some CTV strains were affected by a higher summer temperature, which lead to a change in CTV population structure, and that this effect was host dependent.
[Mh] Termos MeSH primário: Closterovirus/fisiologia
Closterovirus/efeitos da radiação
Doenças das Plantas/virologia
Plantas/virologia
Temperatura Ambiente
[Mh] Termos MeSH secundário: Interações Hospedeiro-Patógeno
Estações do Ano
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170126
[Lr] Data última revisão:
170126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160924
[St] Status:MEDLINE


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[PMID]:27644950
[Au] Autor:Harper SJ; Killiny N; Tatineni S; Gowda S; Cowell SJ; Shilts T; Dawson WO
[Ad] Endereço:Department of Plant Pathology, University of Florida, 700 Experiment Station Road, Lake Alfred, Florida, 33850, USA. sjharper@ufl.edu.
[Ti] Título:Sequence variation in two genes determines the efficacy of transmission of citrus tristeza virus by the brown citrus aphid.
[So] Source:Arch Virol;161(12):3555-3559, 2016 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Vector transmission is an important part of the viral infection cycle, yet for many viruses little is known about this process, or how viral sequence variation affects transmission efficacy. Here we examined the effect of substituting genes from the highly transmissible FS577 isolate of citrus tristeza virus (CTV) in to the poorly transmissible T36-based infectious clone. We found that introducing p65 or p61 sequences from FS577 significantly increased transmission efficacy. Interestingly, replacement of both genes produced a greater increase than either gene alone, suggesting that CTV transmission requires the concerted action of co-evolved p65 and p61 proteins.
[Mh] Termos MeSH primário: Afídeos/virologia
Citrus/virologia
Closterovirus/genética
Insetos Vetores
Doenças das Plantas/virologia
Proteínas Virais/genética
[Mh] Termos MeSH secundário: Animais
Variação Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170126
[Lr] Data última revisão:
170126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160921
[St] Status:MEDLINE


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[PMID]:27599411
[Au] Autor:Read DA; Pietersen G
[Ad] Endereço:Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South Africa. Electronic address: david.read@fabi.up.ac.za.
[Ti] Título:PCR bias associated with conserved primer binding sites, used to determine genotype diversity within Citrus tristeza virus populations.
[So] Source:J Virol Methods;237:107-113, 2016 Nov.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Citrus tristeza virus (CTV) is present in almost all of the major citrus production areas where it continues to reduce the profitability of citriculture. The accurate characterisation of CTV populations, which are usually made up of a number of disparate strains, requires the use of robust PCR protocols. Mismatches between primers and their corresponding binding sites may introduce primer-associated bias during amplification. The primer-associated bias of four sets of CTV specific primers, targeting the A and F regions and the p33 and p23 genes, were evaluated. This was done through the amplification of defined templates followed by their characterisation using the sequencing of multiple clones, as well as Illumina next generation sequencing. High levels of bias were found to be associated with the primer pairs targeting the A and F regions. The p33 gene primers were found to be biased against two genotypes and suggestions for preventing this apparent bias are discussed. The primer pair targeting the conserved p23 gene was found to have very little associated bias. Primers should undergo rigorous screening before being used to characterize virus populations that are known to exhibit high levels of variation, especially within primer binding sites.
[Mh] Termos MeSH primário: Closterovirus/genética
Variação Genética
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Reação em Cadeia da Polimerase
[Mh] Termos MeSH secundário: Sítios de Ligação
Citrus/virologia
Primers do DNA
Genótipo
Sequenciamento de Nucleotídeos em Larga Escala/normas
Filogenia
Doenças das Plantas/virologia
Reação em Cadeia da Polimerase/métodos
Reação em Cadeia da Polimerase/normas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160908
[St] Status:MEDLINE


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[PMID]:27599410
[Au] Autor:Kleynhans J; Pietersen G
[Ad] Endereço:Department of Microbiology and Plant Pathology, University of Pretoria, Private Bag X20, Hatfield, 0028, South Africa; Forestry and Agricultural Biotechnology Institute (FABI) University of Pretoria, Pretoria, 0002, South Africa. Electronic address: jacolenelubbe@gmail.com.
[Ti] Título:Comparison of multiple viral population characterization methods on a candidate cross-protection Citrus tristeza virus (CTV) source.
[So] Source:J Virol Methods;237:92-100, 2016 Nov.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Citrus tristeza virus (CTV) is the most economically important virus found on citrus and influences production worldwide. The 3' half of the RNA genome is generally conserved amongst sources, whereas the 5' portion is more divergent, allowing for the classification of the virus into a number of genotypes based on sequence diversity. The acknowledged genotypes of CTV are continually being expanded, and thus far include T36, T30, T3, VT, B165, HA16-5, T68 and RB. The genotype composition of the CTV populations of a potential cross protection source in Mexican lime was studied whilst comparing different techniques of viral population characterization. Cloning and sequencing of an ORF 1a fragment, genotype specific RT-PCRs and Illumina sequencing of the p33 gene as well as RNA template enrichment through immuno-capture was done. Primers used in the cloning and sequencing proved to be biased towards detection of the VT genotype. RT-PCR and Illumina sequencing using the two different templates provided relatively comparable results, even though the immuno-captured enriched template provided less than expected CTV specific data, while the RT-PCRs and p33 sequencing cannot be used to make inferences about the rest of the genome; which may vary due to recombination. The source was found to contain multiple genotypes, including RB and VT. When choosing a characterization method, the features of the virus under study should be considered. It was found that Illumina sequencing offers an opportunity to gain a large amount of information regarding the entire viral genome, but challenges encountered are discussed.
[Mh] Termos MeSH primário: Citrus/virologia
Closterovirus/genética
Genoma Viral
Análise de Sequência de DNA/métodos
[Mh] Termos MeSH secundário: Closterovirus/imunologia
Proteção Cruzada
Primers do DNA
Variação Genética
Genótipo
Fases de Leitura Aberta
Filogenia
Doenças das Plantas/imunologia
Doenças das Plantas/virologia
RNA Viral/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
Análise de Sequência de DNA/instrumentação
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (RNA, Viral)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160908
[St] Status:MEDLINE


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[PMID]:27520823
[Au] Autor:Killiny N; Harper SJ; Alfaress S; El Mohtar C; Dawson WO
[Ad] Endereço:Plant Pathology Department, CREC-IFAS, University of Florida, Lake Alfred, Florida, USA nabilkilliny@ufl.edu.
[Ti] Título:Minor Coat and Heat Shock Proteins Are Involved in the Binding of Citrus Tristeza Virus to the Foregut of Its Aphid Vector, Toxoptera citricida.
[So] Source:Appl Environ Microbiol;82(21):6294-6302, 2016 Nov 01.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Vector transmission is a critical stage in the viral life cycle, yet for most plant viruses how they interact with their vector is unknown or is explained by analogy with previously described relatives. Here we examined the mechanism underlying the transmission of citrus tristeza virus (CTV) by its aphid vector, Toxoptera citricida, with the objective of identifying what virus-encoded proteins it uses to interact with the vector. Using fluorescently labeled virions, we demonstrated that CTV binds specifically to the lining of the cibarium of the aphid. Through in vitro competitive binding assays between fluorescent virions and free viral proteins, we determined that the minor coat protein is involved in vector interaction. We also found that the presence of two heat shock-like proteins, p61 and p65, reduces virion binding in vitro Additionally, treating the dissected mouthparts with proteases did not affect the binding of CTV virions. In contrast, chitinase treatment reduced CTV binding to the foregut. Finally, competition with glucose, N-acetyl-ß-d-glucosamine, chitobiose, and chitotriose reduced the binding. These findings together suggest that CTV binds to the sugar moieties of the cuticular surface of the aphid cibarium, and the binding involves the concerted activity of three virus-encoded proteins. IMPORTANCE: Limited information is known about the specific interactions between citrus tristeza virus and its aphid vectors. These interactions are important for the process of successful transmission. In this study, we localized the CTV retention site as the cibarium of the aphid foregut. Moreover, we demonstrated that the nature of these interactions is protein-carbohydrate binding. The viral proteins, including the minor coat protein and two heat shock proteins, bind to sugar moieties on the surface of the foregut. These findings will help in understanding the transmission mechanism of CTV by the aphid vector and may help in developing control strategies which interfere with the CTV binding to its insect vector to block the transmission.
[Mh] Termos MeSH primário: Afídeos/virologia
Proteínas do Capsídeo/metabolismo
Closterovirus/metabolismo
Proteínas de Choque Térmico/metabolismo
Proteínas Virais/metabolismo
Ligação Viral
[Mh] Termos MeSH secundário: Animais
Afídeos/anatomia & histologia
Afídeos/metabolismo
Citrus/virologia
Closterovirus/química
Sistema Digestório/virologia
Insetos Vetores/virologia
Microscopia de Polarização
Doenças das Plantas/virologia
Vírion/metabolismo
Vírion/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (Heat-Shock Proteins); 0 (Viral Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160814
[St] Status:MEDLINE


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[PMID]:27405929
[Au] Autor:Liu Z; Chen Z; Hong J; Wang X; Zhou C; Zhou X; Wu J
[Ad] Endereço:State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China.
[Ti] Título:Monoclonal antibody-based serological methods for detecting Citrus tristeza virus in citrus groves.
[So] Source:Virol Sin;31(4):324-30, 2016 Aug.
[Is] ISSN:1995-820X
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:Citrus tristeza virus (CTV) is one of the most economically important citrus viruses and harms the citrus industry worldwide. To develop reliable and effective serological detection assays of CTV, the major capsid protein (CP) gene of CTV was expressed in Escherichia coli BL21 (DE3) using the expression vector pET-28a and purified through Ni+-NTA affinity chromatography. The recombinant protein was used to immunize BALB/c mice. Four hybridoma cell lines (14B10, 14H11, 20D5, and 20G12) secreting monoclonal antibodies (MAbs) against CTV were obtained through conventional hybridoma technology. The titers of MAb-containing ascitic fluids secreted by the four hybridoma lines ranged from 10(-6) to 10(-7) in indirect enzyme-linked immunosorbent assay (ELISA). Western blots showed that all four MAbs could specifically react with CTV CP. Using the prepared MAbs, dot-ELISA, Tissue print-ELISA, and triple antibody sandwich (TAS)-ELISA were developed to detect CTV in tree nurseries and epidemiological studies. The developed dot-ELISA and TAS-ELISA methods could detect CTV in crude extracts of infected citrus leaves with dilutions of 1:2560 and 1:10, 240 (w/v, g/mL), respectively. Tissue print-ELISA was particularly useful for large-scale field sample detection, mainly owing to its simplicity and lack of sample preparation requirements. The field survey revealed that CTV is prevalent on citrus trees in the Chongqing Municipality, Jiangxi Province, and Zhejiang Province of China. The coincidence rate of serological and RT-PCR test results reached more than 99.5%. The prepared MAbs against CTV and established sensitive and specific serological assays have a significant role in the detection and prevention and control of CTV in our country.
[Mh] Termos MeSH primário: Citrus/virologia
Closterovirus/isolamento & purificação
Ensaio de Imunoadsorção Enzimática/métodos
Doenças das Plantas/virologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Monoclonais/análise
Anticorpos Antivirais/análise
Proteínas do Capsídeo/análise
Proteínas do Capsídeo/genética
Proteínas do Capsídeo/imunologia
Closterovirus/genética
Closterovirus/imunologia
Ensaio de Imunoadsorção Enzimática/instrumentação
Imunização
Camundongos
Camundongos Endogâmicos BALB C
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Antibodies, Viral); 0 (Capsid Proteins)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160714
[St] Status:MEDLINE
[do] DOI:10.1007/s12250-016-3718-4


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[PMID]:27208471
[Au] Autor:Meena RP; Baranwal VK
[Ad] Endereço:Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi 110012, India.
[Ti] Título:Development of multiplex polymerase chain reaction assay for simultaneous detection of clostero-, badna- and mandari-viruses along with huanglongbing bacterium in citrus trees.
[So] Source:J Virol Methods;235:58-64, 2016 Sep.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Citrus trees harbor a large number of viral and bacterial pathogens. Citrus yellow vein clearing virus (CYVCV), Indian citrus ringspot virus (ICRSV), Citrus yellow mosaic virus (CYMV), Citrus tristeza virus (CTV) and a bacterium, Candidatus Liberibacter asiaticus (CLa) associated with huanglongbing (HLB) disease, the most prevalent pathogens in citrus orchards of different regions in India and are responsible for debilitating citriculture. For detection of these viral and bacterial pathogens a quick, sensitive and cost effective detection method is required. With this objective a multiplex polymerase chain reaction (mPCR) assay was developed for simultaneous detection of four viruses and a bacterium in citrus. Several sets of primers were designed for each virus based on the retrieved reference sequences from the GenBank. A primer pair published previously was used for greening bacterium. Each pair of primers was evaluated for their sensitivity and differentiation by simplex and mPCR. The constant amplified products were identified on the basis of molecular size in mPCR and were compared with standard PCR. The amplicons were cloned and results were confirmed with sequencing analysis. The mPCR assay was validated using naturally infected field samples for one or more citrus viruses and the huanglongbing bacterium. The mPCR assay developed here will aid in the production of virus free planting materials and rapid indexing for certification of citrus budwood programme.
[Mh] Termos MeSH primário: Citrus/microbiologia
Citrus/virologia
Closterovirus/genética
Flexiviridae/genética
Reação em Cadeia da Polimerase Multiplex/métodos
Rhizobiaceae/genética
[Mh] Termos MeSH secundário: Closterovirus/isolamento & purificação
Primers do DNA
Flexiviridae/isolamento & purificação
Índia
Doenças das Plantas/microbiologia
Doenças das Plantas/prevenção & controle
Doenças das Plantas/virologia
Vírus de Plantas/genética
Rhizobiaceae/isolamento & purificação
Sensibilidade e Especificidade
Árvores/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160522
[St] Status:MEDLINE



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