Base de dados : MEDLINE
Pesquisa : B04.715.260 [Categoria DeCS]
Referências encontradas : 121 [refinar]
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  1 / 121 MEDLINE  
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[PMID]:28864943
[Au] Autor:Grisoni M; Marais A; Filloux D; Saison A; Faure C; Julian C; Theil S; Contreras S; Teycheney PY; Roumagnac P; Candresse T
[Ad] Endereço:Cirad, UMR PVBMT, 97410, Saint Pierre, La Réunion, France. michel.grisoni@cirad.fr.
[Ti] Título:Two novel Alphaflexiviridae members revealed by deep sequencing of the Vanilla (Orchidaceae) virome.
[So] Source:Arch Virol;162(12):3855-3861, 2017 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:The genomes of two novel viruses were assembled from 454 pyrosequencing data obtained from vanilla leaves from La Réunion. Based on genome organization and homologies, one agent was unambiguously classified as a member of the genus Potexvirus and named vanilla virus X (VVX). The second one, vanilla latent virus (VLV), is phylogenetically close to three unclassified members of the family Alphaflexiviridae with similarity to allexiviruses, and despite the presence of an additional 8-kDa open reading frame, we propose to include VLV as a new member of the genus Allexivirus. Both VVX and VLV were mechanically transmitted to vanilla plants, resulting in asymptomatic infections.
[Mh] Termos MeSH primário: Flexiviridae/classificação
Flexiviridae/isolamento & purificação
Potexvirus/classificação
Potexvirus/isolamento & purificação
Análise de Sequência de DNA
Vanilla/virologia
[Mh] Termos MeSH secundário: Flexiviridae/genética
Ordem dos Genes
Genoma Viral
Sequenciamento de Nucleotídeos em Larga Escala
Folhas de Planta/virologia
Potexvirus/genética
Homologia de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170903
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3540-9


  2 / 121 MEDLINE  
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[PMID]:28444538
[Au] Autor:Hu GJ; Dong YF; Zhang ZP; Fan XD; Ren F; Li Z
[Ad] Endereço:Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, 125100, PR China.
[Ti] Título:Occurrence and genetic diversity analysis of apple stem pitting virus isolated from apples in China.
[So] Source:Arch Virol;162(8):2397-2402, 2017 Aug.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Two primer pairs were used to detect apple stem pitting virus (ASPV) using a reverse transcription (RT)-PCR test. 82 out of the 141 randomly collected samples, from ten orchards in five provinces and regions of China, tested positive. In the positive samples forty-five (55%) were infected by ASPV and two other viruses. The full coat protein (CP) and the triple gene block (TGB) gene 1, 2 and 3 of partial ASPV isolates were subsequently cloned. The nucleotide and amino acid identities of 39 CP sequence variants from 31 Chinese apple samples were compared with that of previously reported ASPV isolates and were 67.4-96.0% and 68.4-97.7%, respectively. All ASPV sequence variants from Chinese apples separated into two clades with CP- and TGB-based phylogenetic trees, whilst the grouping of TGB2 and TGB3 trees was the same. Three recombinants (FS06-2, X5-2, and XLF-C-2) for CP and six (TH2-5, X8-2, FS05-2, X6-2 and XLF-A-1) recombinants for TGB were identified from the Chinese apple isolates. Two recombinants were found in the TGB sequence of isolate XLF-A-1. The results presented here may assist in the development of a more comprehensive screening tool for apple viruses.
[Mh] Termos MeSH primário: Flexiviridae/genética
Flexiviridae/isolamento & purificação
Variação Genética
Malus/virologia
Doenças das Plantas/virologia
Caules de Planta/virologia
[Mh] Termos MeSH secundário: China
Primers do DNA
Doenças das Plantas/prevenção & controle
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170427
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3384-3


  3 / 121 MEDLINE  
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[PMID]:28316018
[Au] Autor:Oliveira LM; Orílio AF; Inoue-Nagata AK; Nagata T; Blawid R
[Ad] Endereço:Department Cell Biology, University of Brasília, 70910-900, Brasília, DF, Brazil.
[Ti] Título:A novel vitivirus-like sequence found in Arracacia xanthorrhiza plants by high throughput sequencing.
[So] Source:Arch Virol;162(7):2141-2144, 2017 Jul.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:High throughput sequencing (HTS) is a very powerful tool for detecting and discovering novel viral-like sequences without prior knowledge of the sequence. Here we describe the complete genome of a new vitivirus-like sequence that was found in arracacha (Arracacia xanthorrhiza) plants using HTS technology. The complete genome sequence was validated by Sanger sequencing. The genomic organization of the new putative vitivirus resembles that of grapevine virus B (GVB) and grapevine virus D (GVD). The putative coat protein showed 41 to 49% identity with similar proteins of known vitiviruses, while the RNA-dependent RNA polymerase shared 52 to 55% identity with those encoded by grapevine vitiviruses. Based on the demarcation criteria for the genus Vitivirus, the virus described in this work, provisionally named as "Arracacha virus V", represents a novel species in this taxon.
[Mh] Termos MeSH primário: Apiaceae/virologia
Flexiviridae/classificação
Filogenia
Doenças das Plantas/virologia
[Mh] Termos MeSH secundário: Flexiviridae/genética
Flexiviridae/isolamento & purificação
Genoma Viral
Sequenciamento de Nucleotídeos em Larga Escala
Fases de Leitura Aberta
RNA Replicase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 2.7.7.48 (RNA Replicase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170320
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3326-0


  4 / 121 MEDLINE  
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[PMID]:28274745
[Au] Autor:Cowell SJ; Harper SJ; Dawson WO
[Ad] Endereço:Citrus Research and Education Center, University of Florida, Lake Alfred, FL, USA. Electronic address: sjcowell@ufl.edu.
[Ti] Título:A real-time RT-qPCR assay for the detection of Citrus tatter leaf virus.
[So] Source:J Virol Methods;244:29-31, 2017 Jun.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Citrus tatter leaf virus (CLTV) is globally distributed wherever citrus is grown, and, given the extensive use of CTLV sensitive rootstock, has the potential to be a significant threat to the citrus industry. In order to facilitate fast and reliable detection of this virus, we have developed a CTLV-specific real-time RT-qPCR assay. The optimized assay was found to be more reliable and sensitive compared to ELISA and end-point RT-PCR, detecting CTLV in up to 70% more plants. The real-time RT-qPCR is also specific, as it did not cross-react with the closely related Apple stem grooving virus or with the host itself; robust, being able to detect CTLV in young and mature host tissue types; and rapid.
[Mh] Termos MeSH primário: Flexiviridae/isolamento & purificação
Doenças das Plantas/virologia
Reação em Cadeia da Polimerase em Tempo Real/métodos
[Mh] Termos MeSH secundário: Citrus/virologia
Sensibilidade e Especificidade
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE


  5 / 121 MEDLINE  
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[PMID]:27990565
[Au] Autor:Li Y; Deng C; Bian Y; Zhao X; Zhou Q
[Ad] Endereço:College of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China. lyq@bua.edu.cn.
[Ti] Título:Characterization of apple stem grooving virus and apple chlorotic leaf spot virus identified in a crab apple tree.
[So] Source:Arch Virol;162(4):1093-1097, 2017 Apr.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Apple stem grooving virus (ASGV), apple chlorotic leaf spot virus (ACLSV), and prunus necrotic ringspot virus (PNRSV) were identified in a crab apple tree by small RNA deep sequencing. The complete genome sequence of ACLSV isolate BJ (ACLSV-BJ) was 7554 nucleotides and shared 67.0%-83.0% nucleotide sequence identity with other ACLSV isolates. A phylogenetic tree based on the complete genome sequence of all available ACLSV isolates showed that ACLSV-BJ clustered with the isolates SY01 from hawthorn, MO5 from apple, and JB, KMS and YH from pear. The complete nucleotide sequence of ASGV-BJ was 6509 nucleotides (nt) long and shared 78.2%-80.7% nucleotide sequence identity with other isolates. ASGV-BJ and the isolate ASGV_kfp clustered together in the phylogenetic tree as an independent clade. Recombination analysis showed that isolate ASGV-BJ was a naturally occurring recombinant.
[Mh] Termos MeSH primário: Flexiviridae/isolamento & purificação
Malus/virologia
Doenças das Plantas/virologia
[Mh] Termos MeSH secundário: Sequência de Bases
Flexiviridae/classificação
Flexiviridae/genética
Genoma Viral
Dados de Sequência Molecular
Fases de Leitura Aberta
Filogenia
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170315
[Lr] Data última revisão:
170315
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161220
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-016-3183-2


  6 / 121 MEDLINE  
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[PMID]:27923589
[Au] Autor:Bruisson S; Lebel S; Walter B; Prevotat L; Seddas S; Schellenbaum P
[Ad] Endereço:Université de Haute-Alsace, Laboratoire Vigne Biotechnologies & Environnement, 33 rue de Herrlisheim, 68100, Colmar, France; SEDIAG SAS Company, Technopôle Agro-Environnement, RD 31, 21110 Bretenière, France.
[Ti] Título:Comparative detection of a large population of grapevine viruses by TaqMan RT-qPCR and ELISA.
[So] Source:J Virol Methods;240:73-77, 2017 Feb.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Grapevine (Vitis spp.) can be infected by numerous viruses that are often widespread and of great economic importance. Reliable detection methods are necessary for sanitary selection which is the only way to partly control grapevine virus diseases. Biological indexing and ELISA are currently the standard methods for screening propagation material, and PCR-methods are becoming increasingly popular. Due to the diversity of virus isolates, it is essential to verify that the tests allow the detection of the largest possible virus populations. We developed three quadruplex TaqMan RT-qPCR assays for detecting nine different viruses that cause considerable damage in many vineyards world-wide. Each assay is designed to detect three viruses and the grapevine Actin as an internal control. A large population of grapevines from diverse cultivars and geographic location was tested for the presence of nine viruses: Arabis mosaic virus (ArMV), Grapevine fleck virus (GFkV), Grapevine fanleaf virus (GFLV), Grapevine leafroll-associated viruses (GLRaV-1, -2, -3), Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine virus A (GVA), and Grapevine virus B (GVB). In general, identical results were obtained with multiplex TaqMan RT-qPCR and ELISA although, in some cases, viruses could be detected by only one of the two techniques.
[Mh] Termos MeSH primário: Closteroviridae/isolamento & purificação
Ensaio de Imunoadsorção Enzimática
Flexiviridae/isolamento & purificação
Nepovirus/isolamento & purificação
Reação em Cadeia da Polimerase em Tempo Real
Tymoviridae/isolamento & purificação
Vitis/virologia
[Mh] Termos MeSH secundário: Closteroviridae/genética
Closteroviridae/imunologia
Primers do DNA
DNA Complementar
Flexiviridae/genética
Flexiviridae/imunologia
Nepovirus/genética
Nepovirus/imunologia
Doenças das Plantas/virologia
RNA Viral/isolamento & purificação
Tymoviridae/genética
Tymoviridae/imunologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Complementary); 0 (RNA, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161208
[St] Status:MEDLINE


  7 / 121 MEDLINE  
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[PMID]:27709400
[Au] Autor:Morelli M; Giampetruzzi A; Laghezza L; Catalano L; Savino VN; Saldarelli P
[Ad] Endereço:CNR-Istituto per la Protezione Sostenibile delle Piante (IPSP), Via Amendola 122/D, 70126, Bari, Italy. massimiliano.morelli@ipsp.cnr.it.
[Ti] Título:Identification and characterization of an isolate of apple green crinkle associated virus involved in a severe disease of quince (Cydonia oblonga, Mill.).
[So] Source:Arch Virol;162(1):299-306, 2017 Jan.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:A quince tree showing severe symptoms of a previously undescribed viral disease occurring in northern Apulia (Italy) was analysed using high-throughput sequencing of small RNA libraries, leading to the identification of a new strain of apple green crinkle associated virus (isolate AGCaV-CYD) showing peculiar traits. RT-PCR with specific primers detected AGCaV-CYD in consistent association with symptoms in the surveyed orchards. Molecular characterization of the reconstructed genome, together with phylogenetic analysis, showed it to be closely related to an AGCaV strain causing green crinkle disease in apple (AGCaV-AUR) and divergent from the type strain of apple stem pitting virus (ASPV-PA66).
[Mh] Termos MeSH primário: Flexiviridae/genética
Flexiviridae/isolamento & purificação
Genoma Viral
Doenças das Plantas/virologia
Rosaceae/virologia
[Mh] Termos MeSH secundário: Análise por Conglomerados
Flexiviridae/classificação
Sequenciamento de Nucleotídeos em Larga Escala
Itália
Filogenia
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Homologia de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170127
[Lr] Data última revisão:
170127
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161007
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-016-3074-6


  8 / 121 MEDLINE  
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[PMID]:27928920
[Au] Autor:Komínek P; Komínková M; Jandová B
[Ti] Título:Effect of repeated Ribavirin treatment on grapevine viruses.
[So] Source:Acta Virol;60(4):400-403, 2016.
[Is] ISSN:0001-723X
[Cp] País de publicação:Slovakia
[La] Idioma:eng
[Ab] Resumo:The effect of Ribavirin treatment for the chemotherapy of several grapevine viruses was evaluated. Four grapevine cultivars were repeatedly treated with Ribavirin in two different concentrations and with three different lengths of treatment. Repeating the Ribavirin treatment always had a significant effect on the number of healthy grapevine plants obtained. Ribavirin concentration and length of exposure showed a significant difference in sanitation of the Grapevine rupestris stem pitting-associated virus. During sanitation of the Grapevine Pinot gris virus and Grapevine fleck virus, those two factors did not show significant differences in the elimination of grapevine viruses.
[Mh] Termos MeSH primário: Antivirais/farmacologia
Flexiviridae/efeitos dos fármacos
Doenças das Plantas/prevenção & controle
Ribavirina/farmacologia
Vitis/virologia
[Mh] Termos MeSH secundário: Flexiviridae/fisiologia
Filogenia
Doenças das Plantas/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 49717AWG6K (Ribavirin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170627
[Lr] Data última revisão:
170627
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161209
[St] Status:MEDLINE


  9 / 121 MEDLINE  
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[PMID]:27716257
[Au] Autor:Liu J; Zhang X; Yang Y; Hong N; Wang G; Wang A; Wang L
[Ad] Endereço:State Key Laboratory of Agricultural Microbiology, Wuhan, Hubei, 430070, People's Republic of China.
[Ti] Título:Characterization of virus-derived small interfering RNAs in Apple stem grooving virus-infected in vitro-cultured Pyrus pyrifolia shoot tips in response to high temperature treatment.
[So] Source:Virol J;13(1):166, 2016 Oct 06.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Heat treatment (known as thermotherapy) together with in vitro culture of shoot meristem tips is a commonly used technology to obtain virus-free germplasm for the effective control of virus diseases in fruit trees. RNA silencing as an antiviral defense mechanism has been implicated in this process. To understand if high temperature-mediated acceleration of the host antiviral gene silencing system in the meristem tip facilitates virus-derived small interfering RNAs (vsiRNA) accumulation to reduce the viral RNA titer in the fruit tree meristem tip cells, we used the Apple stem grooving virus (ASGV)-Pyrus pyrifolia pathosystem to explore the possible roles of vsiRNA in thermotherapy. RESULTS: At first we determined the full-length genome sequence of the ASGV-Js2 isolate and then profiled vsiRNAs in the meristem tip of in vitro-grown pear (cv. 'Jinshui no. 2') shoots infected by ASGV-Js2 and cultured at 24 and 37 °C. A total of 7,495 and 7,949 small RNA reads were obtained from the tips of pear shoots cultured at 24 and 37 °C, respectively. Mapping of the vsiRNAs to the ASGV-Js2 genome revealed that they were unevenly distributed along the ASGV-Js2 genome, and that 21- and 22-nt vsiRNAs preferentially accumulated at both temperatures. The 5'-terminal nucleotides of ASGV-specific siRNAs in the tips cultured under different temperatures had a similar distribution pattern, and the nucleotide U was the most frequent. RT-qPCR analyses suggested that viral genome accumulation was drastically compromised at 37 °C compared to 24 °C, which was accompanied with the elevated levels of vsiRNAs at 37 °C. As plant Dicer-like proteins (DCLs), Argonaute proteins (AGOs), and RNA-dependent RNA polymerases (RDRs) are implicated in vsiRNA biogenesis, we also cloned the partial sequences of PpDCL2,4, PpAGO1,2,4 and PpRDR1 genes, and found their expression levels were up-regulated in the ASGV-infected pear shoots at 37 °C. CONCLUSIONS: Collectively, these results showed that upon high temperature treatment, the ASGV-infected meristem shoot tips up-regulated the expression of key genes in the RNA silencing pathway, induced the biogenesis of vsiRNAs and inhibited viral RNA accumulation. This study represents the first report on the characterization of the vsiRNA population in pear plants infected by ASGV-Js2, in response to high temperature treatment.
[Mh] Termos MeSH primário: Flexiviridae/crescimento & desenvolvimento
Temperatura Alta
Brotos de Planta/virologia
Pyrus/virologia
RNA Interferente Pequeno/genética
[Mh] Termos MeSH secundário: Flexiviridae/genética
Flexiviridae/efeitos da radiação
Inativação Gênica
Brotos de Planta/imunologia
Brotos de Planta/efeitos da radiação
Pyrus/imunologia
Pyrus/efeitos da radiação
RNA Interferente Pequeno/metabolismo
RNA Viral/antagonistas & inibidores
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Small Interfering); 0 (RNA, Viral)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161008
[St] Status:MEDLINE


  10 / 121 MEDLINE  
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[PMID]:27604120
[Au] Autor:Fonseca F; Duarte V; Teixeira Santos M; Brazão J; Eiras-Dias E
[Ad] Endereço:CIMA, Universidade do Algarve, Campus de Gambelas, edifício 7, 8005-139, Faro, Portugal. ffonseca@ualg.pt.
[Ti] Título:First molecular characterization of grapevine virus B (GVB) in Portuguese grapevine cultivars and improvement of the RT-PCR detection assay.
[So] Source:Arch Virol;161(12):3535-3540, 2016 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:This work describes the first molecular characterization of grapevine virus B (GVB) in Portuguese grapevine cultivars. During a routine screening of 44 accessions in the National Collection of Grapevine Varieties (CAN PRT051), 17 were found infected with GVB in DAS-ELISA assays with commercial antibodies. However, only six of the corresponding isolates were successfully amplified using primer pairs described in the literature. The sequence variants (ORF4-3'UTR, 1147 nt) retrieved from these isolates segregated into two phylogenetic groups, which included sequences from complete genomes available in GenBank. The highly discrepant results obtained using serological and RT-PCR-based diagnostic tools led to the design of a primer pair for detection of GVB, which allowed the amplification of a 606-bp GVB-specific fragment from all DAS-ELISA-positive isolates and also revealed the existence of false negatives in the serological testing.
[Mh] Termos MeSH primário: Flexiviridae/isolamento & purificação
Variação Genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
Virologia/métodos
Vitis/virologia
[Mh] Termos MeSH secundário: Primers do DNA/genética
Flexiviridae/classificação
Flexiviridae/genética
Fases de Leitura Aberta
Filogenia
Doenças das Plantas/virologia
Portugal
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170126
[Lr] Data última revisão:
170126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160909
[St] Status:MEDLINE



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