Base de dados : MEDLINE
Pesquisa : B04.715.260.150 [Categoria DeCS]
Referências encontradas : 122 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 13 ir para página                         

  1 / 122 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28825206
[Au] Autor:Costa TM; Blawid R; da Costa Junior AC; Lima MF; de Aragão FAS; de Andrade GP; Pio-Ribeiro G; Aranda MA; Inoue-Nagata AK; Nagata T
[Ad] Endereço:Departmento de Biologia Celular, Universidade de Brasília, Brasília, DF, 70910-900, Brazil.
[Ti] Título:Complete genome sequence of melon yellowing-associated virus from melon plants with the severe yellowing disease in Brazil.
[So] Source:Arch Virol;162(12):3899-3901, 2017 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Here, we describe the complete genome sequence of melon yellowing-associated virus (MYaV), found in melon plants with severe yellowing disease, determined by high-throughput and Sanger sequencing. MYaV has an RNA genome of 9073 nucleotides plus a poly(A) tail. At least six open reading frames were predicted, with a typical carlavirus genomic organisation. Phylogenetic analysis of the complete genome sequence and the amino acid sequences of the RNA-dependent RNA polymerase confirmed that MYaV belongs to the genus Carlavirus, with the highest genome-wide nucleotide sequence identity of 59.8% to sweet potato yellow mottle virus.
[Mh] Termos MeSH primário: Carlavirus/classificação
Carlavirus/isolamento & purificação
Cucurbitaceae/virologia
Genoma Viral
Doenças das Plantas/virologia
Análise de Sequência de DNA
[Mh] Termos MeSH secundário: Brasil
Carlavirus/genética
Fases de Leitura Aberta
Filogenia
RNA Viral/genética
Vírus Satélites
Homologia de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170822
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3532-9


  2 / 122 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27730428
[Au] Autor:Carvalho SL; Nagata T; Junqueira BR; Zanardo LG; Paiva AC; Carvalho CM
[Ad] Endereço:Department of Phytopathology, Universidade Federal de Viçosa, Av. P. H. Rolfs, s/n Campus Universitário, Viçosa, MG, 36570-900, Brazil.
[Ti] Título:Construction of a full-length infectious cDNA clone of Cowpea mild mottle virus.
[So] Source:Virus Genes;53(1):137-140, 2017 Feb.
[Is] ISSN:1572-994X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Infectious cDNA clones are an important tool to study the molecular and cellular process of RNA virus infection. In vitro and in vivo transcription systems are the two main strategies used in the generation of infectious cDNA clones for RNA viruses. This study describes the first generation of a full-length infectious cDNA clone of Cowpea mild mottle virus (CPMMV), a Carlavirus. The full-length genome was synthesized by Overlap Extension PCR of two overlapping fragments and cloned in a pUC-based vector under control of the SP6 RNA polymerase promoter. After in vitro run-off transcription, the produced RNA was mechanically inoculated into soybean plants cv. CD206. The systemic infection was confirmed by RT-PCR and further sequencing of amplified cDNA fragments. To simplify the transfection process, the complete genome was subcloned into a binary vector under control of the 35S promoter of cauliflower mosaic virus by the Gibson Assembly protocol. The resulting clones were inoculated by particle bombardment onto soybean seedlings and the recovery of the virus was confirmed 2 weeks later by RT-PCR. Our results indicate the constructs of the full-length cDNA of CPMMV are fully infectious in both in vitro and in vivo transcription strategies.
[Mh] Termos MeSH primário: Carlavirus/genética
DNA Complementar
Genoma Viral
[Mh] Termos MeSH secundário: Clonagem Molecular
Ordem dos Genes
Fases de Leitura Aberta
Doenças das Plantas/virologia
Feijão de Soja/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161013
[St] Status:MEDLINE
[do] DOI:10.1007/s11262-016-1395-x


  3 / 122 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28005969
[Au] Autor:Tugume AK; Mukasa SB; Valkonen JP
[Ad] Endereço:Department of Agricultural Sciences, Faculty of Agriculture and Forestry, University of Helsinki, Helsinki, Finland.
[Ti] Título:Mixed Infections of Four Viruses, the Incidence and Phylogenetic Relationships of Sweet Potato Chlorotic Fleck Virus (Betaflexiviridae) Isolates in Wild Species and Sweetpotatoes in Uganda and Evidence of Distinct Isolates in East Africa.
[So] Source:PLoS One;11(12):e0167769, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Viruses infecting wild flora may have a significant negative impact on nearby crops, and vice-versa. Only limited information is available on wild species able to host economically important viruses that infect sweetpotatoes (Ipomoea batatas). In this study, Sweet potato chlorotic fleck virus (SPCFV; Carlavirus, Betaflexiviridae) and Sweet potato chlorotic stunt virus (SPCSV; Crinivirus, Closteroviridae) were surveyed in wild plants of family Convolvulaceae (genera Astripomoea, Ipomoea, Hewittia and Lepistemon) in Uganda. Plants belonging to 26 wild species, including annuals, biannuals and perennials from four agro-ecological zones, were observed for virus-like symptoms in 2004 and 2007 and sampled for virus testing. SPCFV was detected in 84 (2.9%) of 2864 plants tested from 17 species. SPCSV was detected in 66 (5.4%) of the 1224 plants from 12 species sampled in 2007. Some SPCSV-infected plants were also infected with Sweet potato feathery mottle virus (SPFMV; Potyvirus, Potyviridae; 1.3%), Sweet potato mild mottle virus (SPMMV; Ipomovirus, Potyviridae; 0.5%) or both (0.4%), but none of these three viruses were detected in SPCFV-infected plants. Co-infection of SPFMV with SPMMV was detected in 1.2% of plants sampled. Virus-like symptoms were observed in 367 wild plants (12.8%), of which 42 plants (11.4%) were negative for the viruses tested. Almost all (92.4%) the 419 sweetpotato plants sampled from fields close to the tested wild plants displayed virus-like symptoms, and 87.1% were infected with one or more of the four viruses. Phylogenetic and evolutionary analyses of the 3'-proximal genomic region of SPCFV, including the silencing suppressor (NaBP)- and coat protein (CP)-coding regions implicated strong purifying selection on the CP and NaBP, and that the SPCFV strains from East Africa are distinguishable from those from other continents. However, the strains from wild species and sweetpotato were indistinguishable, suggesting reciprocal movement of SPCFV between wild and cultivated Convolvulaceae plants in the field.
[Mh] Termos MeSH primário: Carlavirus/isolamento & purificação
Crinivirus/isolamento & purificação
Ipomoea batatas/virologia
Potyvirus/isolamento & purificação
[Mh] Termos MeSH secundário: Regiões 3' não Traduzidas/genética
África Oriental
Capsídeo/metabolismo
Carlavirus/classificação
Carlavirus/metabolismo
Coinfecção/virologia
Crinivirus/classificação
Crinivirus/metabolismo
Evolução Molecular
Incidência
Ipomoea batatas/crescimento & desenvolvimento
Filogenia
Doenças das Plantas/etiologia
Doenças das Plantas/virologia
Potyvirus/classificação
Potyvirus/metabolismo
Recombinação Genética
Uganda
Proteínas Virais/genética
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3' Untranslated Regions); 0 (Viral Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170703
[Lr] Data última revisão:
170703
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161223
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0167769


  4 / 122 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27737784
[Au] Autor:He X; Xue F; Xu S; Wang W
[Ad] Endereço:Beijing Engineering Research Center of Rural Landscape Planning and Design, College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China; Beijing Collaborative Innovation Center for Eco-Environmental Improvement with Forestry and Fruit Trees, Beijing 102206, China. Ele
[Ti] Título:Rapid and sensitive detection of Lily symptomless virus by reverse transcription loop-mediated isothermal amplification.
[So] Source:J Virol Methods;238:38-41, 2016 Dec.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Lily symptomless virus (LSV) is one of the most prevalent viruses that infect lily plants worldwide. A rapid and sensitive reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detection of LSV, using two primer pairs that specifically amplified the conserved sequence of LSV coat protein. The optimum reaction conditions were as follows: 4mM MgCl and 0.8M betaine with incubation at 64°C for 30min. The limit of detection of LSV from infected lily leaves was 10-fold higher for RT-LAMP than for conventional RT-PCR. Moreover, RT-LAMP detected LSV in not only symptomatic, but also in symptomless tissues of infected plants. These findings indicate that our RT-LAMP method for LSV can serve as a low-cost, simple, and rapid alternative to conventional detection assays.
[Mh] Termos MeSH primário: Carlavirus/genética
Carlavirus/isolamento & purificação
Lilium/virologia
Técnicas de Amplificação de Ácido Nucleico/métodos
Transcrição Reversa
[Mh] Termos MeSH secundário: Primers do DNA
Limite de Detecção
Técnicas de Amplificação de Ácido Nucleico/economia
Doenças das Plantas/virologia
RNA Viral/análise
Sensibilidade e Especificidade
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (RNA, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161015
[St] Status:MEDLINE


  5 / 122 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27614753
[Au] Autor:Igori D; Lim S; Zhao F; Baek D; Park JM; Cho HS; Kim HS; Kwon SY; Moon JS
[Ad] Endereço:Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, 34113, Republic of Korea.
[Ti] Título:The complete sequence and genome organization of ligustrum virus A, a novel carlavirus.
[So] Source:Arch Virol;161(12):3593-3596, 2016 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:The complete genome sequence of ligustrum virus A (LVA) from a Ligustrum obtusifolium Sieb. & Zucc. plant was determined. The genomic RNA has 8,525 nucleotides, excluding the poly(A) tail, and consists of six open reading frames typical of members of the genus Carlavirus, family Betaflexiviridae. Phylogenetic analysis of the viral replicase and coat protein (CP) indicated that LVA is closely related to daphne virus S and helenium virus. The replicase and CP of LVA shared 44.73-52.35 % and 25.39-62.46 % amino acid identity, respectively, with those of other carlaviruses. These results suggest that LVA is a member of a distinct carlavirus species.
[Mh] Termos MeSH primário: Carlavirus/genética
Carlavirus/isolamento & purificação
Ordem dos Genes
Genoma Viral
Ligustrum/virologia
RNA Viral/genética
Análise de Sequência de DNA
[Mh] Termos MeSH secundário: Carlavirus/classificação
Análise por Conglomerados
Fases de Leitura Aberta
Filogenia
Homologia de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170126
[Lr] Data última revisão:
170126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160912
[St] Status:MEDLINE


  6 / 122 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26851177
[Au] Autor:Ho T; Quito-Avila D; Keller KE; Postman JD; Martin RR; Tzanetakis IE
[Ad] Endereço:Department of Plant Pathology, Division of Agriculture, University of Arkansas System, Fayetteville, AR 72701, USA.
[Ti] Título:Evidence of sympatric speciation of elderberry carlaviruses.
[So] Source:Virus Res;215:72-5, 2016 Apr 02.
[Is] ISSN:1872-7492
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Five new carlaviruses infecting elderberry were characterized and tentatively named as elderberry virus A-E (ElVA-ElVE). Their genome organization is similar to that of other carlaviruses with size ranging from 8540 to 8628 nucleotides, excluding the polyadenylated tails. ElVA, ElVB and ElVD share a common ancestor as do ElVC and ElVE, indicating that speciation may be sympatric with all viruses having emerged in elderberry. Analyses of the carlavirus conserved domains indicate that the 2-oxoglutarate and Fe(II)-dependent oxygenase motifs are reliable indicators of virus phylogenetic classification with recombination playing a significant role in the evolution of the genus. A universal RT-PCR assay that detects all the elderberry carlaviruses and potentially other members of the genus has been developed. This tool can be used for research and regulatory purposes as elderberry cultivation is rapidly expanding to new areas where the viruses may be absent.
[Mh] Termos MeSH primário: Carlavirus/classificação
Carlavirus/genética
Especiação Genética
Sambucus/virologia
[Mh] Termos MeSH secundário: Ordem dos Genes
Genoma Viral
Filogenia
Recombinação Genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160207
[St] Status:MEDLINE


  7 / 122 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26381670
[Au] Autor:Nemchinov L
[Ad] Endereço:USDA/ARS, Plant Sciences Institute, Molecular Plant Pathology Laboratory, Beltsville, MD, 20705, USA. Lev.Nemchinov@ars.usda.gov.
[Ti] Título:Comment on "The complete nucleotide sequence and genome organization of pea streak virus (genus Carlavirus)".
[So] Source:Arch Virol;160(10):2655, 2015 Oct.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Mh] Termos MeSH primário: Carlavirus/genética
Carlavirus/isolamento & purificação
Genoma Viral
Ervilhas/virologia
Doenças das Plantas/virologia
[Pt] Tipo de publicação:COMMENT; LETTER
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150926
[Lr] Data última revisão:
150926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150919
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-015-2594-9


  8 / 122 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26374561
[Au] Autor:Dietzgen RG
[Ad] Endereço:Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, Brisbane, QLD, 4072, Australia. r.dietzgen@uq.edu.au.
[Ti] Título:Response from Ralf Dietzgen to "Comment on The complete nucleotide sequence and genome organization of pea streak virus (genus Carlavirus)".
[So] Source:Arch Virol;160(10):2657, 2015 Oct.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Mh] Termos MeSH primário: Carlavirus/genética
Carlavirus/isolamento & purificação
Genoma Viral
Ervilhas/virologia
Doenças das Plantas/virologia
[Pt] Tipo de publicação:COMMENT; LETTER
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150926
[Lr] Data última revisão:
150926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150917
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-015-2595-8


  9 / 122 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26374560
[Au] Autor:Xiang Y
[Ad] Endereço:Agriculture and Agri-Food Canada, Pacific Agri-Food, Research Centre, Summerland, BC, V0H 1Z0, Canada. yu.xiang@agr.gc.ca.
[Ti] Título:Response from Yu Xiang to "Comment on The complete nucleotide sequence and genome organization of pea streak virus (genus Carlavirus)".
[So] Source:Arch Virol;160(10):2659, 2015 Oct.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Mh] Termos MeSH primário: Carlavirus/genética
Carlavirus/isolamento & purificação
Genoma Viral
Ervilhas/virologia
Doenças das Plantas/virologia
[Pt] Tipo de publicação:COMMENT; LETTER
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150926
[Lr] Data última revisão:
150926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150917
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-015-2596-7


  10 / 122 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26346833
[Au] Autor:Kalnciema I; Balke I; Skrastina D; Ose V; Zeltins A
[Ad] Endereço:Latvian Biomedical Research and Study Centre, Ratsupites 1, Riga, 1067, Latvia. kalnciema@biomed.lu.lv.
[Ti] Título:Potato Virus M-Like Nanoparticles: Construction and Characterization.
[So] Source:Mol Biotechnol;57(11-12):982-92, 2015 Dec.
[Is] ISSN:1559-0305
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Virus-like particles (VLPs) are multisubunit self-assembly competent protein structures with identical or highly related overall structure to their corresponding native viruses. To construct a new filamentous VLP carrier, the coat protein (CP) gene from potato virus M (PVM) was amplified from infected potato plants, cloned, and expressed in Escherichia coli cells. As demonstrated by electron microscopy analysis, the PVM CP self-assembles into filamentous PVM-like particles, which are mostly 100-300 nm in length. Adding short Gly-Ser peptide at the C-terminus of the PVM, CP formed short VLPs, whereas peptide and protein A Z-domain fusions at the CP N-terminus retained its ability to form typical PVM VLPs. The PVM-derived VLP carrier accommodates up to 78 amino acid-long foreign sequences on its surface and can be produced in technologically significant amounts. PVM-like particles are stable at physiological conditions and also, apparently do not become disassembled in high salt and high pH solutions as well as in the presence of EDTA or reducing agents. Despite partial proteolytic processing of doubled Z-domain fused to PVM VLPs, the rabbit IgGs specifically bind to the particles, which demonstrates the functional activity and surface location of the Z-domain in the PVM VLP structure. Therefore, PVM VLPs may be recognized as powerful structural blocks for new human-made nanomaterials.
[Mh] Termos MeSH primário: Carlavirus/genética
Genoma Viral
Nanopartículas/virologia
Vacinas de Partículas Semelhantes a Vírus/química
[Mh] Termos MeSH secundário: Animais
Carlavirus/isolamento & purificação
Carlavirus/fisiologia
Clonagem Molecular
DNA Complementar/genética
DNA Complementar/metabolismo
Escherichia coli/genética
Concentração de Íons de Hidrogênio
Imunoglobulina G/sangue
Imunoglobulina G/química
Coelhos
Solanum tuberosum/virologia
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Vacinas de Partículas Semelhantes a Vírus/isolamento & purificação
Montagem de Vírus
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Immunoglobulin G); 0 (Vaccines, Virus-Like Particle)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150909
[St] Status:MEDLINE
[do] DOI:10.1007/s12033-015-9891-0



página 1 de 13 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde