Base de dados : MEDLINE
Pesquisa : B04.715.780 [Categoria DeCS]
Referências encontradas : 115 [refinar]
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[PMID]:28468626
[Au] Autor:Xu Q; Liu H; Yuan P; Zhang X; Chen Q; Jiang X; Zhou Y
[Ad] Endereço:Institute of Plant Protection; Jiangsu Academy of Agricultural Sciences; Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing, Jiangsu, People's Republic of China.
[Ti] Título:Development of a simplified RT-PCR without RNA isolation for rapid detection of RNA viruses in a single small brown planthopper (Laodelphax striatellus Fallén).
[So] Source:Virol J;14(1):90, 2017 05 03.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The small brown planthopper (SBPH) is an important pest of cereal crops and acts as a transmission vector for multiple RNA viruses. Rapid diagnosis of virus in the vector is crucial for efficient forecast and control of viral disease. Reverse transcription polymerase chain reaction (RT-PCR) is a rapid, sensitive and reliable method for virus detection. The traditional RT-PCR contains a RNA isolation step and is widely used for virus detection in insect. However, using the traditional RT-PCR for detecting RNA virus in individual SBPHs becomes challenging because of the expensive reagents and laborious procedure associated with RNA isolation when processing a large number of samples. RESULTS: We established a simplified RT-PCR method without RNA isolation for RNA virus detection in a single SBPH. This method is achieved by grinding a single SBPH in sterile water and using the crude extract directly as the template for RT-PCR. The crude extract containing the virus RNA can be prepared in approximately two minutes. Rice stripe virus (RSV), rice black streaked dwarf virus (RBSDV) and Himetobi P virus (HiPV) were successfully detected using this simplified method. The detection results were validated by sequencing and dot immunobinding assay, indicating that this simplified method is reliable for detecting different viruses in insects. The evaluation of the sensitivity of this method showed that both RSV and HiPV can be detected when the cDNA from the crude extract was diluted up to 10 fold. Compared to the traditional RT-PCR with RNA isolation, the simplified RT-PCR method greatly reduces the sample processing time, decreases the detection cost, and improves the efficiency by avoiding RNA isolation. CONCLUSIONS: A simplified RT-PCR method is developed for rapid detection of RNA virus in a single SBPH without the laborious RNA isolation step. It offers a convenient alternative to the traditional RT-PCR method.
[Mh] Termos MeSH primário: Hemípteros/virologia
Insetos Vetores/virologia
Vírus de RNA/isolamento & purificação
RNA Viral/isolamento & purificação
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
[Mh] Termos MeSH secundário: Animais
DNA Complementar/isolamento & purificação
Dicistroviridae/genética
Dicistroviridae/isolamento & purificação
Immunoblotting/métodos
Insetos/virologia
Vírus de Plantas/genética
Vírus de Plantas/isolamento & purificação
RNA Viral/análise
Reoviridae/genética
Reoviridae/isolamento & purificação
Sensibilidade e Especificidade
Tenuivirus/genética
Tenuivirus/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (RNA, Viral)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1186/s12985-017-0732-6


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[PMID]:28471518
[Au] Autor:Li S; Zhou C; Chen G; Zhou Y
[Ad] Endereço:Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
[Ti] Título:Bacterial microbiota in small brown planthopper populations with different rice viruses.
[So] Source:J Basic Microbiol;57(7):590-596, 2017 Jul.
[Is] ISSN:1521-4028
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The small brown planthopper (SBPH) is an important virus vector, transmitting Rice stripe virus (RSV), and Rice black-streaked dwarf virus (RBSDV). Insect symbionts play an essential role in the insect fitness, however, it is still unclear about their contributions to viral transmission by SBPH. Here, we investigated endosymbiont communities in non-viruliferous, RSV-infected, and RBSDV-infected SBPH populations using Illumina 16S rRNA gene MiSeq sequencing. In total, 281,803 effective sequences of the 16S rRNA gene were generated from different samples. Sequence analysis revealed the percentages of these bacterial groups in different SBPH populations on several taxonomic levels ranging from phyla to genera. The extremely consistent bacterial diversity and abundance indicated that RSV or RBSDV infection did not affect the composition and abundance of symbionts in SBPH. It was notable that Wolbachia was dominant in all populations. The symbiosis between Wolbachia and SBPH might be potentially studied and utilized to control pest SBPH in the future.
[Mh] Termos MeSH primário: Bactérias/isolamento & purificação
Hemípteros/microbiologia
Insetos Vetores/microbiologia
Microbiota
Simbiose
[Mh] Termos MeSH secundário: Animais
Bactérias/classificação
Bactérias/genética
Hemípteros/virologia
Sequenciamento de Nucleotídeos em Larga Escala
Insetos Vetores/virologia
Microbiota/genética
Oryza
Vírus de Plantas/isolamento & purificação
Vírus de Plantas/fisiologia
RNA Ribossômico 16S
Tenuivirus/isolamento & purificação
Tenuivirus/fisiologia
Wolbachia/genética
Wolbachia/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180117
[Lr] Data última revisão:
180117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1002/jobm.201700004


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[PMID]:28977024
[Au] Autor:Zheng L; Zhang C; Shi C; Yang Z; Wang Y; Zhou T; Sun F; Wang H; Zhao S; Qin Q; Qiao R; Ding Z; Wei C; Xie L; Wu J; Li Y
[Ad] Endereço:State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, China.
[Ti] Título:Rice stripe virus NS3 protein regulates primary miRNA processing through association with the miRNA biogenesis factor OsDRB1 and facilitates virus infection in rice.
[So] Source:PLoS Pathog;13(10):e1006662, 2017 Oct.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:MicroRNAs (miRNAs) are small regulatory RNAs processed from primary miRNA transcripts, and plant miRNAs play important roles in plant growth, development, and response to infection by microbes. Microbial infections broadly alter miRNA biogenesis, but the underlying mechanisms remain poorly understood. In this study, we report that the Rice stripe virus (RSV)-encoded nonstructural protein 3 (NS3) interacts with OsDRB1, an indispensable component of the rice (Oryza sativa) miRNA-processing complex. Moreover, the NS3-OsDRB1 interaction occurs at the sites required for OsDRB1 self-interaction, which is essential for miRNA biogenesis. Further analysis revealed that NS3 acts as a scaffold between OsDRB1 and pri-miRNAs to regulate their association and aids in vivo processing of pri-miRNAs. Genetic evidence in Arabidopsis showed that NS3 can partially substitute for the function of double-stranded RNA binding domain (dsRBD) of AtDRB1/AtHYL1 during miRNA biogenesis. As a result, NS3 induces the accumulation of several miRNAs, most of which target pivotal genes associated with development or pathogen resistance. In contrast, a mutant version of NS3 (mNS3), which still associated with OsDRB1 but has defects in pri-miRNA binding, reduces accumulation of these miRNAs. Transgenic rice lines expressing NS3 exhibited significantly higher susceptibility to RSV infection compared with non-transgenic wild-type plants, whereas the transgenic lines expressing mNS3 showed a less-sensitive response. Our findings revealed a previously unknown mechanism in which a viral protein hijacks OsDRB1, a key component of the processing complex, for miRNA biogenesis and enhances viral infection and pathogenesis in rice.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica de Plantas/genética
MicroRNAs/genética
Oryza/virologia
Proteínas de Ligação a RNA/metabolismo
Tenuivirus/genética
Proteínas Virais/metabolismo
[Mh] Termos MeSH secundário: Oryza/genética
Interferência de RNA/fisiologia
Proteínas de Ligação a RNA/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs); 0 (RNA-Binding Proteins); 0 (Viral Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171005
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006662


  4 / 115 MEDLINE  
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[PMID]:28359901
[Au] Autor:Lu G; Li J; Zhou Y; Zhou X; Tao X
[Ad] Endereço:Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, PR China.
[Ti] Título:Model-based structural and functional characterization of the Rice stripe tenuivirus nucleocapsid protein interacting with viral genomic RNA.
[So] Source:Virology;506:73-83, 2017 Jun.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Rice stripe tenuivirus (RSV) is a filamentous, negative-strand RNA virus causing severe diseases on rice in Asian countries. The viral particle is composed predominantly of a nucleocapsid protein (NP) and genomic RNA. However, the molecular details of how the RSV NP interacts with genomic RNA during particle assembly remain largely unknown. Here, we modeled the NP-RNA complex and show that polar amino acids within a predicted groove of NP are critical for RNA binding and protecting the RNA from RNase digestion. RSV NP formed pentamers, hexamers, heptamers, and octamers. By modeling the higher-order structures, we found that oligomer formation was driven by the N-terminal amino arm of the NP. Deletion of this arm abolished oligomerization; the N-terminally truncated NP was less able to interact with RNA and protect RNA than was the wild type. These findings afford valuable new insights into molecular mechanism of RSV NPs interacting with genomic RNA.
[Mh] Termos MeSH primário: Nucleocapsídeo/metabolismo
Oryza/virologia
Doenças das Plantas/virologia
RNA Viral/metabolismo
Tenuivirus/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Modelos Moleculares
Dados de Sequência Molecular
Nucleocapsídeo/química
Nucleocapsídeo/genética
Ligação Proteica
RNA Viral/química
RNA Viral/genética
Alinhamento de Sequência
Tenuivirus/química
Tenuivirus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170705
[Lr] Data última revisão:
170705
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170401
[St] Status:MEDLINE


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[PMID]:28189616
[Au] Autor:He M; Guan SY; He CQ
[Ad] Endereço:College of Life Science, Shandong Normal University, Jinan 250014, China; College of Life Science, Northeast Forestry University, Harbin 150040, China.
[Ti] Título:Evolution of rice stripe virus.
[So] Source:Mol Phylogenet Evol;109:343-350, 2017 Apr.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Rice stripe virus (RSV) is an insect-borne tenuivirus of economical significance. It is endemic to the rice-growing regions of East Asia and exhibits more genetic diversity in Yunnan Province of China. To gain more insights into the molecular epidemiology and evolution of RSV, recombination analyses were conducted and potential events were detected in each of the four RNA segments of RSV. Bayesian coalescent method was then applied to the time-stamped coding sequences of the CP gene. The nucleotide substitution rate and the divergence time were estimated. Age calculations suggested that the first diversification event of the RSV isolates analyzed might take place in the early 20th century, and RSV has existed in Yunnan long before notice. Surveys of codon usage variation showed that the RSV genes had influences other than mutational bias. In codon choice, RSV conformed to neither vector small brown planthopper nor host rice, although the former exerted a more dominant influence on shaping codon usage pattern of RSV. In addition, CpG dinucleotide deficiency was observed in RSV.
[Mh] Termos MeSH primário: Evolução Molecular
Tenuivirus/classificação
[Mh] Termos MeSH secundário: Teorema de Bayes
China
Códon
Variação Genética
Oryza/virologia
Filogenia
Tenuivirus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Codon)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170213
[St] Status:MEDLINE


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[PMID]:28130458
[Au] Autor:Jiao WJ; Li FQ; Bai YL; Shi XX; Zhu MF; Zhang MJ; Mao CG; Zhu ZR
[Ad] Endereço:State Key Laboratory of Rice Biology/Key Laboratory of Agricultural Entomology Ministry of Agriculture/Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China zrzhu@zju.edu.cn.
[Ti] Título:Rice Stripe Virus Infection Alters mRNA Levels of Sphingolipid-Metabolizing Enzymes and Sphingolipids Content in Laodelphax striatellus.
[So] Source:J Insect Sci;17(1), 2017 Jan.
[Is] ISSN:1536-2442
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sphingolipids and their metabolites have been implicated in viral infection and replication in mammal cells but how their metabolizing enzymes in the host are regulated by viruses remains largely unknown. Here we report the identification of 12 sphingolipid genes and their regulation by Rice stripe virus in the small brown planthopper (Laodelphax striatellus Fallén), a serious pest of rice throughout eastern Asia. According to protein sequence similarity, we identified 12 sphingolipid enzyme genes in L. striatellus. By comparing their mRNA levels in viruliferous versus nonviruliferous L. striatellus at different life stages by qPCR, we found that RSV infection upregulated six genes (LsCGT1, LsNAGA1, LsSGPP, LsSMPD4, LsSMS, and LsSPT) in most stages of L. striatellus Especially, four genes (LsCGT1, LsSMPD2, LsNAGA1, and LsSMS) and another three genes (LsNAGA1, LsSGPP, and LsSMS) were significantly upregulated in viruliferous third-instar and fourth-instar nymphs, respectively. HPLC-MS/MS results showed that RSV infection increased the levels of various ceramides, such as Cer18:0, Cer20:0, and Cer22:0 species, in third and fourth instar L. striatellus nymphs. Together, these results demonstrate that RSV infection alters the transcript levels of various sphingolipid enzymes and the contents of sphingolipids in L. striatellus, indicating that sphingolipids may be important for RSV infection or replication in L. striatellus.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica
Hemípteros/genética
Hemípteros/virologia
Proteínas de Insetos/genética
Esfingolipídeos/genética
Tenuivirus/fisiologia
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida de Alta Pressão
Feminino
Hemípteros/enzimologia
Hemípteros/metabolismo
Proteínas de Insetos/metabolismo
Masculino
Ninfa/enzimologia
Ninfa/genética
Ninfa/metabolismo
Ninfa/virologia
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Análise de Sequência de RNA
Esfingolipídeos/metabolismo
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (RNA, Messenger); 0 (Sphingolipids)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170129
[St] Status:MEDLINE


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[PMID]:27912765
[Au] Autor:Sun F; Fang P; Li J; Du L; Lan Y; Zhou T; Fan Y; Shen W; Zhou Y
[Ad] Endereço:Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences; Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing, 210014, China.
[Ti] Título:RNA-seq-based digital gene expression analysis reveals modification of host defense responses by rice stripe virus during disease symptom development in Arabidopsis.
[So] Source:Virol J;13(1):202, 2016 Dec 02.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Virus infection induces and suppresses host gene expression on a global level. Rice stripe virus (RSV) is the type species of the genus Tenuivirus and infects rice and Arabidopsis plants. Microarray-based and next generation sequencing-based transcriptomic approaches have been used to study rice-RSV interactions. However, our knowledge of the response of Arabidopsis plants to RSV infection is limited, and it requires further investigation to determine the similarities (or differences) in virus-host interactions between monocot and dicot hosts infected with RSV. METHODS: We characterized transcriptome changes in Arabidopsis thaliana infected with rice stripe virus (RSV) with RNA-seq based digital gene expression (DGE) analysis. The transcriptomes of RSV-infected samples were compared to those of mock-treated samples at 14 and 21 days post-infection (dpi) during different stages of symptom development. RESULTS: We identified 624 differentially expressed genes (DEGs) in Arabidopsis influenced by RSV at 14 dpi and 21 dpi, among which at 14 dpi, 255 transcripts were induced, and 38 were repressed; at 21 dpi, 146 were induced, and 237 were repressed. Functional annotation indicated that these DEGs were related to multiple biological functions, including defense response, secondary metabolism, protein amino acid phosphorylation and response to abiotic stress. CONCLUSIONS: Importantly, the transcription of genes related to host defense systems was activated by RSV infection at an early stage of symptom development (14 dpi), whereas over the infection period (21 dpi), the host defense response systems were suppressed. A total of 52 genes were continuously differentially expressed between the two time points, indicating that the majority of DEGs were transient and unique to a particular time point during symptom development. The DEGs, particularly the defense response genes, identified in this study are candidates suitable for further functional analysis during the RSV-Arabidopsis interaction.
[Mh] Termos MeSH primário: Arabidopsis/virologia
Perfilação da Expressão Gênica/métodos
Interações Hospedeiro-Patógeno
Doenças das Plantas/virologia
Análise de Sequência de RNA/métodos
Tenuivirus/crescimento & desenvolvimento
Tenuivirus/genética
[Mh] Termos MeSH secundário: Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161204
[St] Status:MEDLINE


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[PMID]:27393974
[Au] Autor:Liu X; Xiong G; Qiu P; Du Z; Kormelink R; Zheng L; Zhang J; Ding X; Yang L; Zhang S; Wu Z
[Ad] Endereço:Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
[Ti] Título:Inherent properties not conserved in other tenuiviruses increase priming and realignment cycles during transcription of Rice stripe virus.
[So] Source:Virology;496:287-298, 2016 Sep.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Two tenuiviruses Rice stripe virus (RSV) and Rice grassy stunt virus (RGSV) were found to co-infect rice with the same reovirus Rice ragged stunt virus (RRSV). During the co-infection, both tenuiviruses recruited 10-21 nucleotides sized capped-RNA leaders from the RRSV. A total of 245 and 102 RRSV-RGSV and RRSV-RSV chimeric mRNA clones, respectively, were sequenced. An analysis of the sequences suggested a scenario consistent with previously reported data on related viruses, in which capped leader RNAs having a 3' end complementary to the viral template are preferred and upon base pairing the leaders prime processive transcription directly or after one to several cycles of priming and realignment (repetitive prime-and-realign). Interestingly, RSV appeared to have a higher tendency to use repetitive prime-and-realign than RGSV even with the same leader derived from the same RRSV RNA. Combining with relevant data reported previously, this points towards an intrinsic feature of RSV.
[Mh] Termos MeSH primário: Oryza/virologia
Tenuivirus/genética
Transcrição Genética
[Mh] Termos MeSH secundário: Regiões 5' não Traduzidas
Sequência de Bases
Coinfecção
Evolução Molecular
Doenças das Plantas/virologia
RNA Mensageiro/genética
RNA Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (5' Untranslated Regions); 0 (RNA, Messenger); 0 (RNA, Viral)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160710
[St] Status:MEDLINE


  9 / 115 MEDLINE  
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[PMID]:27283882
[Au] Autor:Huo Y; Chen L; Su L; Wu Y; Chen X; Fang R; Zhang L
[Ad] Endereço:State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; National Plant Gene Research Center, Beijing, 100101, China.
[Ti] Título:Artificial feeding Rice stripe virus enables efficient virus infection of Laodelphax striatellus.
[So] Source:J Virol Methods;235:139-43, 2016 Sep.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Rice stripe virus (RSV), the causative agent of rice stripe disease, is transmitted by Laodelphax striatellus in a persistent-propagative manner. Efficient virus acquisition is primary for studies of virus transmission and virus-insect vector interactions. However, under greenhouse conditions, less than 30% of the L. striatellus population, on average, become viruliferous during feeding on RSV-infected plants. Here, we explored a method for efficient RSV acquisition by feeding the insects with a virus-containing artificial diet. Virus particles were partially purified from frozen infected rice leaves. A series of RSV concentrations in a 5% sucrose solution were tested in the feed of L. striatellus nymphs. The percentage of infected insects increased along with the increasing viral concentration, and the highest infection percentage 96% was achieved using a 1200ngµL(-1) crude RSV suspension after 48h feeding. RSV particles acquired in this manner were able to spread to L. striatellus salivary glands. This improved method of obtaining viruliferous insects should assist the study of RSV transmission mechanisms in L. striatellus.
[Mh] Termos MeSH primário: Hemípteros/virologia
Insetos Vetores/virologia
Doenças das Plantas/virologia
Tenuivirus/fisiologia
Vírion/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Apoio Nutricional
Oryza/virologia
Folhas de Planta/virologia
Tenuivirus/química
Tenuivirus/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160611
[St] Status:MEDLINE


  10 / 115 MEDLINE  
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[PMID]:26991800
[Au] Autor:Zhang F; Li Q; Chen X; Huo Y; Guo H; Song Z; Cui F; Zhang L; Fang R
[Ad] Endereço:State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
[Ti] Título:Roles of the Laodelphax striatellus Down syndrome cell adhesion molecule in Rice stripe virus infection of its insect vector.
[So] Source:Insect Mol Biol;25(4):413-21, 2016 Aug.
[Is] ISSN:1365-2583
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The arthropod Down syndrome cell adhesion molecule (Dscam) mediates pathogen-specific recognition via an extensive protein isoform repertoire produced by alternative splicing. To date, most studies have focused on the subsequent pathogen-specific immune response, and few have investigated the entry into cells of viruses or endosymbionts. In the present study, we cloned and characterized the cDNA of Laodelphax striatellus Dscam (LsDscam) and investigated the function of LsDscam in rice stripe virus (RSV) infection and the influence on the endosymbiont Wolbachia. LsDscam displayed a typical Dscam domain architecture, including 10 immunoglobulin (Ig) domains, six fibronectin type III domains, one transmembrane domain and a cytoplasmic tail. Alternative splicing occurred at the N-termini of the Ig2 and Ig3 domains, the complete Ig7 domain, the transmembrane domain and the C-terminus, comprising 10, 51, 35, two and two variable exons, respectively. Potentially LsDscam could encode at least 71 400 unique isoforms and 17 850 types of extracellular regions. LsDscam was expressed in various L. striatellus tissues. Knockdown of LsDscam mRNA via RNA interference decreased the titres of both RSV and Wolbachia, but did not change the numbers of the extracellular symbiotic bacterium Acinetobacter rhizosphaerae. Specific Dscam isoforms may play roles in enhancing the infection of vector-borne viruses or endosymbionts.
[Mh] Termos MeSH primário: Hemípteros/microbiologia
Hemípteros/fisiologia
Proteínas de Insetos/genética
Moléculas de Adesão de Célula Nervosa/genética
Oryza/virologia
Doenças das Plantas/virologia
Tenuivirus/fisiologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Clonagem Molecular
DNA Complementar/genética
DNA Complementar/metabolismo
Hemípteros/genética
Hemípteros/crescimento & desenvolvimento
Proteínas de Insetos/química
Proteínas de Insetos/metabolismo
Insetos Vetores/genética
Insetos Vetores/crescimento & desenvolvimento
Insetos Vetores/microbiologia
Insetos Vetores/fisiologia
Moléculas de Adesão de Célula Nervosa/química
Moléculas de Adesão de Célula Nervosa/metabolismo
Ninfa/genética
Ninfa/crescimento & desenvolvimento
Ninfa/microbiologia
Ninfa/fisiologia
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Alinhamento de Sequência
Simbiose
Wolbachia/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Insect Proteins); 0 (Neural Cell Adhesion Molecules); 0 (RNA, Messenger)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160319
[St] Status:MEDLINE
[do] DOI:10.1111/imb.12226



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