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[PMID]:28410608
[Au] Autor:Domanska-Blicharz K; Bocian L; Lisowska A; Jacukowicz A; Pikula A; Minta Z
[Ad] Endereço:Department of Poultry Diseases, National Veterinary Research Institute, Al. Partyzantów 57, 24-100, Pulawy, Poland. domanska@piwet.pulawy.pl.
[Ti] Título:Cross-sectional survey of selected enteric viruses in Polish turkey flocks between 2008 and 2011.
[So] Source:BMC Vet Res;13(1):108, 2017 Apr 14.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Enteric diseases are an important health problem for the intensive poultry industry, resulting in considerable economic losses. Apart from such microbiological agents associated with enteritis as bacteria and parasites, a lot of research has been recently conducted on viral origin of enteric diseases. However, enteric viruses have been identified in intestinal tract of not only diseased but also healthy poultry, so their role in enteritis is still unclear. The present study aimed at determination of the prevalence of four enteric viruses, namely astrovirus, coronavirus, parvovirus and rotavirus in meat-type turkey flocks in Poland as well as at statistical evaluation of the occurrence of the studied viruses and their relationships with the health status and the age of birds. Two hundred and seven flocks of birds aged 1-20 weeks originating from different regions of the country were investigated between 2008 and 2011. Clinical samples (10 individual faecal swabs/flock) were duly processed and examined using molecular methods targeting the conservative regions of viral genomes: RNA-dependent RNA polymerase gene of astrovirus, non-structural 1 gene of parvovirus, non-structural protein 4 gene of rotavirus, and 5' untranslated region fragment of turkey coronavirus. Different statistical methods (i.e. the independence chi-square test, the correspondence analysis and the logistic regression model) were used to establish any relationships between the analyzed data. RESULTS: Overall, 137 (66.2%, 95% CI: 59.3-72.6) of the 207 turkey flocks sampled were infected with one or more enteric viruses. Among the 137 flocks, 74 (54%, 95% CI: 45.3-62.6) were positive for one virus, whereas 54 (39.4%, 9 5% CI: 31.2-48.1) and 9 (6.6%, 95% CI: 3.1-12.1) were co-infected with two or three different enteric viruses, respectively. No flock was simultaneously infected with all four viruses studied. The prevalence of astrovirus infection was 44.9% (95% CI: 38.0-52.0), parvovirus 27.5% (95% CI: 21.6-34.2), rotavirus 18.8% (95% CI: 13.8-24.8), and coronavirus 9.7% (95% CI: 6.0-14.5). Young turkeys aged 1-4 weeks old had the highest (82.1%, 95% CI:71.7-89.8) prevalence of viral infection. Applied statistical methods have indicated the dependence of rotavirus infection as well as the co-infection with multiple viruses and the health status of turkeys. Furthermore, our results statistically confirm that especially young birds are susceptible to infection with rotavirus and astrovirus. CONCLUSIONS: The study demonstrated the presence of astrovirus, coronavirus, parvovirus and rotavirus infections in Polish turkey farms. These viruses were detected in both healthy and diseased birds. However, the presented results provide valuable feedback which could help to evaluate the role of some enteric viruses in the etiology of enteritis in turkey.
[Mh] Termos MeSH primário: Fezes/virologia
Doenças das Aves Domésticas/virologia
Perus/virologia
[Mh] Termos MeSH secundário: Fatores Etários
Animais
Avastrovirus/genética
Avastrovirus/isolamento & purificação
Coinfecção/veterinária
Coinfecção/virologia
Coronavirus/genética
Coronavirus/isolamento & purificação
Estudos Transversais
Enterite/veterinária
Enterite/virologia
Parvovirus/genética
Parvovirus/isolamento & purificação
Polônia/epidemiologia
Rotavirus/genética
Rotavirus/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170416
[St] Status:MEDLINE
[do] DOI:10.1186/s12917-017-1013-8


  2 / 81 MEDLINE  
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[PMID]:28316017
[Au] Autor:Zhang Y; Wang F; Liu N; Yang L; Zhang D
[Ad] Endereço:College of Life Science, South China Agricultural University, Guangzhou, People's Republic of China.
[Ti] Título:Complete genome sequence of a novel avastrovirus in goose.
[So] Source:Arch Virol;162(7):2135-2139, 2017 Jul.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:We report the complete genome sequence of a new avastrovirus of goose-origin (FLX). The 7299-nt-long genome consisted of three overlapping open reading frames (ORFs) that were in different reading frames. Pairwise comparisons showed that the FLX genome was 59% identical to its closest relatives and that the levels of amino acid identity shared by FLX with other astroviruses did not exceed 54% in ORF1a, 66% in ORF1b, and 50% in ORF2, respectively. Phylogenetic analysis based on the amino acid sequence of the full-length ORF2 demonstrated that FLX was highly divergent from all other avastroviruses. At the amino acid level the complete capsid region of FLX shared genetic distances of 0.574-0.719 with three official avastrovirus species, suggesting that it can be classified as a member of a novel species in the genus Avastrovirus.
[Mh] Termos MeSH primário: Infecções por Astroviridae/virologia
Avastrovirus/classificação
Gansos/virologia
Filogenia
Doenças das Aves Domésticas/virologia
[Mh] Termos MeSH secundário: Animais
Avastrovirus/genética
Avastrovirus/isolamento & purificação
Proteínas do Capsídeo/genética
China
Genoma Viral
Fases de Leitura Aberta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsid Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170320
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3297-1


  3 / 81 MEDLINE  
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[PMID]:28208602
[Au] Autor:Smyth VJ
[Ad] Endereço:Agri-Food and Biosciences Institute, Stormont Laboratory, Stoney Road, Belfast, BT4 3SD, UK. victoria.smyth@afbini.gov.uk.
[Ti] Título:A Review of the Strain Diversity and Pathogenesis of Chicken Astrovirus.
[So] Source:Viruses;9(2), 2017 Feb 10.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Although a relatively recently emerged virus, identified only in 2004 as a separate species of avian astrovirus, chicken astrovirus (CAstV) has been associated with poor growth of broiler flocks, enteritis and diarrhea and is a candidate pathogen in cases of runting stunting syndrome. More recently CAstV has been implicated in cases of two other diseases of broilers as the sole etiological agent, namely severe kidney disease of young broilers with visceral gout and the "White Chicks" hatchery disease. Examination of the strains of CAstV associated with the two latter diseases reveals they are closely related genetically. This review will discuss the pathogenesis of CAstV in relation to strain diversity and the effects of vertical versus horizontal transmission, virus load, co-infections and age of bird at infection, all factors that may impact upon disease severity.
[Mh] Termos MeSH primário: Avastrovirus/classificação
Avastrovirus/patogenicidade
Variação Genética
Doenças das Aves Domésticas/patologia
Doenças das Aves Domésticas/virologia
[Mh] Termos MeSH secundário: Animais
Galinhas
Transmissão de Doença Infecciosa
Doenças das Aves Domésticas/transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170218
[St] Status:MEDLINE


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[PMID]:28012117
[Au] Autor:Patel AK; Pandit RJ; Thakkar JR; Hinsu AT; Pandey VC; Pal JK; Prajapati KS; Jakhesara SJ; Joshi CG
[Ad] Endereço:Research and Development, Hester Biosciences Ltd, Ahmedabad, 380006, Gujarat, India. amrutkpatel@gmail.com.
[Ti] Título:Complete genome sequence analysis of chicken astrovirus isolate from India.
[So] Source:Vet Res Commun;41(1):67-75, 2017 Mar.
[Is] ISSN:1573-7446
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Chicken astroviruses have been known to cause severe disease in chickens leading to increased mortality and "white chicks" condition. Here we aim to characterize the causative agent of visceral gout suspected for astrovirus infection in broiler breeder chickens. METHODS: Total RNA isolated from allantoic fluid of SPF embryo passaged with infected chicken sample was sequenced by whole genome shotgun sequencing using ion-torrent PGM platform. The sequence was analysed for the presence of coding and non-coding features, its similarity with reported isolates and epitope analysis of capsid structural protein. RESULTS: The consensus length of 7513 bp genome sequence of Indian isolate of chicken astrovirus was obtained after assembly of 14,121 high quality reads. The genome was comprised of 13 bp 5'-UTR, three open reading frames (ORFs) including ORF1a encoding serine protease, ORF1b encoding RNA dependent RNA polymerase (RdRp) and ORF2 encoding capsid protein, and 298 bp of 3'-UTR which harboured two corona virus stem loop II like "s2m" motifs and a poly A stretch of 19 nucleotides. The genetic analysis of CAstV/INDIA/ANAND/2016 suggested highest sequence similarity of 86.94% with the chicken astrovirus isolate CAstV/GA2011 followed by 84.76% with CAstV/4175 and 74.48%% with CAstV/Poland/G059/2014 isolates. The capsid structural protein of CAstV/INDIA/ANAND/2016 showed 84.67% similarity with chicken astrovirus isolate CAstV/GA2011, 81.06% with CAstV/4175 and 41.18% with CAstV/Poland/G059/2014 isolates. However, the capsid protein sequence showed high degree of sequence identity at nucleotide level (98.64-99.32%) and at amino acids level (97.74-98.69%) with reported sequences of Indian isolates suggesting their common origin and limited sequence divergence. The epitope analysis by SVMTriP identified two unique epitopes in our isolate, seven shared epitopes among Indian isolates and two shared epitopes among all isolates except Poland isolate which carried all distinct epitopes.
[Mh] Termos MeSH primário: Avastrovirus/genética
Genoma Viral/genética
[Mh] Termos MeSH secundário: Animais
Avastrovirus/classificação
Avastrovirus/isolamento & purificação
Galinhas
Epitopos/genética
Índia
Homologia de Sequência do Ácido Nucleico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Epitopes)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161225
[St] Status:MEDLINE
[do] DOI:10.1007/s11259-016-9673-6


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[PMID]:27928915
[Au] Autor:Kaithal B; Jindal N; Kumar P; Mor SK
[Ti] Título:Detection and molecular characterization of enteric viruses in enteritis-affected commercial broiler chickens in India.
[So] Source:Acta Virol;60(4):361-371, 2016.
[Is] ISSN:0001-723X
[Cp] País de publicação:Slovakia
[La] Idioma:eng
[Ab] Resumo:A study was conducted to detect and characterize the enteric viruses (chicken astrovirus, avian nephritis virus and avian orthoreovirus) present in flocks of commercial broiler chickens suffering from enteritis in Haryana, India. The intestinal contents were collected from 65 enteritis-affected flocks (cases) and tested by reverse transcription PCR (RT-PCR). Of these 65 cases, 35 (53.80%) were positive for a single virus and 26 (40.00%) for two viruses. The remaining four samples were negative for all three viruses tested. Of the 65 cases, 57 were positive for chicken astrovirus (CAstV) while 30 cases had avian nephritis virus (ANV). None of the cases were positive for orthoreovirus. Comparison of 12 CAstVs of this study with previously published CAstV sequences revealed nucleotide identities ranging from 73.20 to 98.00%. The nucleotide identities ranged between 83.10-95.50% when nine ANVs of this study were compared with previously reported ANV sequences. The amino acid sequences of CAstVs in comparison to previously published sequences revealed certain unique changes. Phylogeny based on polymerase gene revealed that CAstVs and ANVs of this study were under the same monophyletic clade. In conclusion, a large number of broiler chicken flocks experiencing enteritis were positive for CAstV and ANV by RT-PCR. The presence of more than one enteric virus in enteritis-affected flocks and changes at the genetic level in these viruses may affect the severity of disease.
[Mh] Termos MeSH primário: Infecções por Astroviridae/veterinária
Avastrovirus/isolamento & purificação
Enterite/veterinária
Orthoreovirus Aviário/isolamento & purificação
Doenças das Aves Domésticas/virologia
Infecções por Reoviridae/veterinária
[Mh] Termos MeSH secundário: Animais
Infecções por Astroviridae/epidemiologia
Infecções por Astroviridae/virologia
Avastrovirus/classificação
Avastrovirus/genética
Galinhas/virologia
Enterite/virologia
Índia/epidemiologia
Orthoreovirus Aviário/classificação
Orthoreovirus Aviário/genética
Filogenia
Doenças das Aves Domésticas/epidemiologia
Infecções por Reoviridae/epidemiologia
Infecções por Reoviridae/virologia
Perus/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170627
[Lr] Data última revisão:
170627
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161209
[St] Status:MEDLINE


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[PMID]:27803180
[Au] Autor:Meliopoulos VA; Marvin SA; Freiden P; Moser LA; Nighot P; Ali R; Blikslager A; Reddivari M; Heath RJ; Koci MD; Schultz-Cherry S
[Ad] Endereço:Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
[Ti] Título:Oral Administration of Astrovirus Capsid Protein Is Sufficient To Induce Acute Diarrhea In Vivo.
[So] Source:MBio;7(6), 2016 Nov 01.
[Is] ISSN:2150-7511
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The disease mechanisms associated with the onset of astrovirus diarrhea are unknown. Unlike other enteric virus infections, astrovirus infection is not associated with an inflammatory response or cellular damage. In vitro studies in differentiated Caco-2 cells demonstrated that human astrovirus serotype 1 (HAstV-1) capsid protein alone disrupts the actin cytoskeleton and tight junction complex, leading to increased epithelial barrier permeability. In this study, we show that oral administration of purified recombinant turkey astrovirus 2 (TAstV-2) capsid protein results in acute diarrhea in a dose- and time-dependent manner in turkey poults. Similarly to that induced by infectious virus, TAstV-2 capsid-induced diarrhea was independent of inflammation or histological changes but was associated with increased intestinal barrier permeability, as well as redistribution of sodium hydrogen exchanger 3 (NHE3) from the membrane to the cytoplasm of the intestinal epithelium. Unlike other viral enterotoxins that have been identified, astrovirus capsid induces diarrhea after oral administration, reproducing the natural route of infection and demonstrating that ingestion of intact noninfectious capsid protein may be sufficient to provoke acute diarrhea. Based on these data, we hypothesize that the astrovirus capsid acts like an enterotoxin and induces intestinal epithelial barrier dysfunction. IMPORTANCE: Acute gastroenteritis, with its sequela diarrhea, is one of the most important causes of childhood morbidity and mortality worldwide. A variety of infectious agents cause gastroenteritis, and in many cases, an enterotoxin produced by the agent is involved in disease manifestations. Although we commonly think of bacteria as a source of toxins, at least one enteric virus, rotavirus, produces a protein with enterotoxigenic activity during viral replication. In these studies, we demonstrate that oral administration of the turkey astrovirus 2 (TAstV-2) structural (capsid) protein induces acute diarrhea, increases barrier permeability, and causes relocalization of NHE3 in the small intestine, suggesting that rotavirus may not be alone in possessing enterotoxigenic activity.
[Mh] Termos MeSH primário: Avastrovirus/patogenicidade
Proteínas do Capsídeo/administração & dosagem
Proteínas do Capsídeo/toxicidade
Diarreia/induzido quimicamente
Diarreia/patologia
[Mh] Termos MeSH secundário: Administração Oral
Membrana Celular/química
Citoplasma/química
Mucosa Intestinal/patologia
Trocadores de Sódio-Hidrogênio/análise
Turquia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (Sodium-Hydrogen Exchangers)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161103
[St] Status:MEDLINE


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[PMID]:27638777
[Au] Autor:Espinoza LL; Beserra LA; Soares RM; Gregori F
[Ad] Endereço:Department of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo, Av. Professor Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, SP, Brazil. lunaluis@usp.br.
[Ti] Título:Avian nephritis virus (ANV) on Brazilian chickens farms: circulating genotypes and intra-genotypic diversity.
[So] Source:Arch Virol;161(12):3455-3462, 2016 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Avian nephritis virus (ANV), which belongs to the family Astroviridae, is associated with different clinical manifestations (including enteric disorders). Despite being frequently found in the avian industry worldwide, information regarding genetic features of these viruses in Brazil is scarce. Therefore, sixty fecal sample pools (5-6 birds of the same flock), representing 60 poultry farms from six Brazilian States, were screened using an astrovirus-specific hemi-nested-PCR assay targeting the conserved ORF1b gene, followed by nucleotide sequencing of amplified products. PCR and phylogenetic analysis confirmed the detection of 21 positive samples to ANV (35 %). In order to investigate the genetic diversity represented by these viruses, amplification, cloning and phylogenetic analysis of the deduced amino acid sequence of ORF2 gene were attempted. Eight samples were successfully cloned (generating 32 clones in total) and sequenced. Based on phylogenetic analysis of ORF2, sequences defined in this study were classified into three genotypes: genotype 5, which has already been described in birds, and two other novel genotypes, tentatively named genotype 8 and 9, all of which occurred in single or mixed infections. Moreover, high intra-genotypic diversity and co-circulation of distinct strains in a same host population were observed. This study revealed the presence of new strains of ANV in Brazilian poultry and their circulation in commercial chicken flocks.
[Mh] Termos MeSH primário: Infecções por Astroviridae/veterinária
Avastrovirus/classificação
Avastrovirus/genética
Variação Genética
Genótipo
Doenças das Aves Domésticas/virologia
[Mh] Termos MeSH secundário: Animais
Infecções por Astroviridae/virologia
Brasil
Galinhas
Análise por Conglomerados
Fazendas
Fases de Leitura Aberta
Filogenia
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Homologia de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170126
[Lr] Data última revisão:
170126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160918
[St] Status:MEDLINE


  8 / 81 MEDLINE  
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[PMID]:27610731
[Au] Autor:Espinoza LL; Beserra LA; Soares RM; Gregori F
[Ad] Endereço:Department of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo, Av. Professor Dr. Orlando Marques de Paiva, 87, CEP: 05508-270, Cidade Universitária, São Paulo, SP, Brazil.
[Ti] Título:Turkey Astrovirus Type 1 (TAstV-1) and Chicken Astrovirus (CAstV) Detection in Brazilian Chicken Flocks.
[So] Source:Avian Dis;60(3):681-7, 2016 Sep.
[Is] ISSN:1938-4351
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Astrovirus is a common cause of enteritis in humans and domestic animals. Here we report the detection of turkey astrovirus type 1 (TAstV-1) and chicken astrovirus (CAstV) in avian farms. Sixty fecal sample pools (five or six birds of the same flock), from chickens without apparent clinical symptoms of enteric disease from farms located in six Brazilian states, were screened by an ORF1b PCR, followed by nucleotide sequencing of amplified products and phylogenetic analysis. Six samples tested positive for TAstV-1 and two for CAstV. One positive sample of each detected virus (TAstV-1 and CAstV) had the complete ORF2 sequenced. Data for the ORF2 sequence indicate that Brazilian TAstV-1 was divergent from TAstV-1 (United States), previously described infecting turkeys, and Brazilian CAstV clustered together with the U.K. group, subgroup B-II, associated with enteritis and growth retardation in chicks. This study provides updated information about CAstV and the first report of detection of TAstV-1 in Brazilian chickens, supporting the diagnostic of enteritis and epidemiologic surveillance in poultry health.
[Mh] Termos MeSH primário: Infecções por Astroviridae/veterinária
Avastrovirus/isolamento & purificação
Galinhas
Fases de Leitura Aberta
Doenças das Aves Domésticas/epidemiologia
[Mh] Termos MeSH secundário: Animais
Infecções por Astroviridae/epidemiologia
Infecções por Astroviridae/virologia
Avastrovirus/genética
Brasil/epidemiologia
Filogenia
Doenças das Aves Domésticas/virologia
Análise de Sequência de RNA/veterinária
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160910
[St] Status:MEDLINE
[do] DOI:10.1637/11403-030816-ResNote.1


  9 / 81 MEDLINE  
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[PMID]:27339687
[Au] Autor:Sajewicz-Krukowska J; Domanska-Blicharz K
[Ad] Endereço:Department of Poultry Diseases, National Veterinary Research Institute, Al. Partyzantow 57, 24-100, Pulawy, Poland.
[Ti] Título:Nearly full-length genome sequence of a novel astrovirus isolated from chickens with 'white chicks' condition.
[So] Source:Arch Virol;161(9):2581-7, 2016 Sep.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Avian astroviruses (aAstVs) are divided into three species, Avastrovirus 1, Avastrovirus 2, and Avastrovirus 3, but there are a few strains are waiting to be assigned to an official taxonomic group. This study presents the molecular characterization of chicken astrovirus (CAstV), PL/G059/2014, which is involved in the induction of "white chicks" condition. The 7382-nucleotide-long genome sequence was determined by next-generation sequencing using an Illumina MiSeq System. Phylogenetic analysis showed that it has the characteristics that are typical of avian astroviruses. However, overall degree of nucleotide sequence identity was 43.6 % to 73.7 % between PL/G059/2014 and other available genome sequences of aAstV strains. The amino acid sequences of the proteins encoded by ORF1a and ORF1b of the studied strain were very similar (86.5-93.8 % identity) to those of CAstVs 4175 and GA2011, but they were only 32.7-35.2 % identical in the case of ORF2, which is used officially for astrovirus species demarcation. These features could suggest that the PL/G059/2014 strain should be assigned to a new species in the genus Avastrovirus. Moreover, the different phylogenetic topology of PL/G059/2014 and its nucleotide sequence similarity in different genomic regions could suggest that a recombination event occurred during its evolution and that it has ancestors in common with duck astroviruses.
[Mh] Termos MeSH primário: Infecções por Astroviridae/veterinária
Avastrovirus/genética
Avastrovirus/isolamento & purificação
Genoma Viral
Doenças das Aves Domésticas/virologia
[Mh] Termos MeSH secundário: Animais
Infecções por Astroviridae/virologia
Galinhas
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160625
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-016-2940-6


  10 / 81 MEDLINE  
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[PMID]:26976903
[Au] Autor:Nuñez LF; Santander Parra SH; Carranza C; Astolfi-Ferreira CS; Buim MR; Piantino Ferreira AJ
[Ad] Endereço:Department of Pathology, School of Veterinary Medicine, University of São Paulo (USP), Av. Prof. Dr. Orlando M. Paiva, 87, CEP 05508-270, São Paulo, SP, Brazil.
[Ti] Título:Detection and molecular characterization of chicken astrovirus associated with chicks that have an unusual condition known as "white chicks" in Brazil.
[So] Source:Poult Sci;95(6):1262-70, 2016 Jun 01.
[Is] ISSN:1525-3171
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chicken astrovirus (CAstV) is one of many viruses related to enteric diseases in poultry that are associated with Runting-Stunting Syndrome (RSS), which affects young chickens. CAstV was also recently associated with an unusual condition in chicks called "white chicks." Some hatcheries in certain states of Brazil have reported several incubation problems, mortality, and the presence of chicks with white plumages over the past several months. These chicks were termed locally as "white chicks." The present work investigated 30 chicks with this unusual condition using a multidisciplinary approach. Postmortem examination of each chick showed enlarged livers and intestines that were full of liquid and gas (30/30). The pancreas, kidneys, and spleen were pale (30/30). The other organs did not show any macroscopic alterations. CAstV, chicken parvovirus (ChPV), avian nephritis virus (ANV), avian rotavirus (ARtV), avian reovirus (AReoV), infectious bronchitis virus (IBV), and fowl adenovirus group I (FAdV-1) were tested in the intestines, pancreas, proventriculus, gizzard, liver, spleen, bursa, kidneys, thymus, lung, heart, brain, and yolk sac in each chick. All organs and yolk sacs were positive for CAstV in different titres and negative for the other tested viruses. The partial molecular characterization of the ORF 1b gene of CAstV using 28 sequences revealed a high similarity of the nucleotides and amino acids with sequences of CAstV from North America, Europe, and Asia, and our CAstV sequences clustered into a unique group that was separate from the other sequences. These results demonstrated that CAstV was associated with the white chick condition in Brazil. The virus was distributed in most organs, including the brain and yolk sac. These results suggest that the virus could be transmitted vertically. The molecular characterization also revealed that the CAstV associated with white chick condition was molecularly related to other CAstV sequences found worldwide.
[Mh] Termos MeSH primário: Infecções por Astroviridae/veterinária
Avastrovirus/fisiologia
Galinhas
Genes Virais
Fases de Leitura Aberta
Doenças das Aves Domésticas/virologia
[Mh] Termos MeSH secundário: Animais
Infecções por Astroviridae/classificação
Infecções por Astroviridae/virologia
Avastrovirus/genética
Brasil
Filogenia
Doenças das Aves Domésticas/classificação
Análise de Sequência de RNA/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160316
[St] Status:MEDLINE
[do] DOI:10.3382/ps/pew062



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