Base de dados : MEDLINE
Pesquisa : B04.820.069.500 [Categoria DeCS]
Referências encontradas : 605 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 61 ir para página                         

  1 / 605 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28982120
[Au] Autor:Gonzales-Gustavson E; Timoneda N; Fernandez-Cassi X; Caballero A; Abril JF; Buti M; Rodriguez-Frias F; Girones R
[Ad] Endereço:Laboratory of Virus Contaminants of Water and Food, Department of Genetics, Microbiology and Statistics, Faculty of Biology, University of Barcelona, Barcelona, Catalonia, Spain.
[Ti] Título:Identification of sapovirus GV.2, astrovirus VA3 and novel anelloviruses in serum from patients with acute hepatitis of unknown aetiology.
[So] Source:PLoS One;12(10):e0185911, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hepatitis is a general term meaning inflammation of the liver, which can be caused by a variety of viruses. However, a substantial number of cases remain with unknown aetiology. We analysed the serum of patients with clinical signs of hepatitis using a metagenomics approach to characterize their viral species composition. Four pools of patients with hepatitis without identified aetiological agents were evaluated. Additionally, one pool of patients with hepatitis E (HEV) and pools of healthy volunteers were included as controls. A high diversity of anelloviruses, including novel sequences, was found in pools from patients with hepatitis of unknown aetiology. Moreover, viruses recently associated with gastroenteritis as sapovirus GV.2 and astrovirus VA3 were also detected only in those pools. Besides, most of the HEV genome was recovered from the HEV pool. Finally, GB virus C and human endogenous retrovirus were found in the HEV and healthy pools. Our study provides an overview of the virome in serum from hepatitis patients suggesting a potential role of these viruses not previously described in cases of hepatitis. However, further epidemiologic studies are necessary to confirm their contribution to the development of hepatitis.
[Mh] Termos MeSH primário: Anelloviridae/isolamento & purificação
Hepatite Viral Humana/virologia
Mamastrovirus/isolamento & purificação
Sapovirus/isolamento & purificação
Viremia/sangue
[Mh] Termos MeSH secundário: Doença Aguda
Anelloviridae/classificação
Estudos de Casos e Controles
Hepatite Viral Humana/sangue
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Mamastrovirus/classificação
Filogenia
Viremia/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185911


  2 / 605 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Miagostovich, Marize Pereira
Leite, José Paulo Gagliardi
Texto completo
[PMID]:28854225
[Au] Autor:Portes SAR; Carvalho-Costa FA; Rocha MS; Fumian TM; Maranhão AG; de Assis RM; Xavier MDPTP; Rocha MS; Miagostovich MP; Leite JPG; Volotão EM
[Ad] Endereço:Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Rio de Janeiro, Brazil.
[Ti] Título:Enteric viruses in HIV-1 seropositive and HIV-1 seronegative children with diarrheal diseases in Brazil.
[So] Source:PLoS One;12(8):e0183196, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Diarrheal diseases (DD) have distinct etiological profiles in immune-deficient and immune-competent patients. This study compares detection rates, genotype distribution and viral loads of different enteric viral agents in HIV-1 seropositive (n = 200) and HIV-1 seronegative (n = 125) children hospitalized with DD in Rio de Janeiro, Brazil. Except for group A rotavirus (RVA), which were detected through enzyme immunoassay, the other enteric viruses (norovirus [NoV], astrovirus [HAstV], adenovirus [HAdV] and bocavirus [HBoV]) were detected through PCR or RT-PCR. A quantitative PCR was performed for RVA, NoV, HAstV, HAdV and HBoV. Infections with NoV (19% vs. 9.6%; p<0.001), HBoV (14% vs. 7.2%; p = 0.042) and HAdV (30.5% vs. 14.4%; p<0.001) were significantly more frequent among HIV-1 seropositive children. RVA was significantly less frequent among HIV-1 seropositive patients (6.5% vs. 20%; p<0.001). Similarly, frequency of infection with HAstV was lower among HIV-1 seropositive children (5.5% vs. 12.8%; p = 0.018). Among HIV-1 seropositive children 33 (16.5%) had co-infections, including three enteric viruses, such as NoV, HBoV and HAdV (n = 2) and NoV, HAstV and HAdV (n = 2). The frequency of infection with more than one virus was 17 (13.6%) in the HIV-1 negative group, triple infection (NoV + HAstV + HBoV) being observed in only one patient. The median viral load of HAstV in feces was significantly higher among HIV-1 positive children compared to HIV-1 negative children. Concerning children infected with RVA, NoV, HBoV and HAdV, no statistically significant differences were observed in the medians of viral loads in feces, comparing HIV-1 seropositive and HIV-1 seronegative children. Similar detection rates were observed for RVA, HAstV and HAdV, whilst NoV and HBoV were significantly more prevalent among children with CD4+ T lymphocyte count below 200 cells/mm3. Enteric viruses should be considered an important cause of DD in HIV-1 seropositive children, along with pathogens more classically associated with intestinal infections in immunocompromised hosts.
[Mh] Termos MeSH primário: Infecções por Adenoviridae/epidemiologia
Infecções por Astroviridae/epidemiologia
Infecções por Caliciviridae/epidemiologia
Diarreia/epidemiologia
Gastroenterite/epidemiologia
Infecções por HIV/epidemiologia
Infecções por Parvoviridae/epidemiologia
Infecções por Rotavirus/epidemiologia
[Mh] Termos MeSH secundário: Adenoviridae/crescimento & desenvolvimento
Adenoviridae/isolamento & purificação
Infecções por Adenoviridae/imunologia
Infecções por Adenoviridae/virologia
Infecções por Astroviridae/imunologia
Infecções por Astroviridae/virologia
Brasil/epidemiologia
Contagem de Linfócito CD4
Linfócitos T CD4-Positivos/imunologia
Linfócitos T CD4-Positivos/virologia
Infecções por Caliciviridae/imunologia
Infecções por Caliciviridae/virologia
Criança
Pré-Escolar
Coinfecção
Diarreia/imunologia
Diarreia/virologia
Fezes/virologia
Feminino
Gastroenterite/imunologia
Gastroenterite/virologia
Infecções por HIV/imunologia
Infecções por HIV/virologia
HIV-1/crescimento & desenvolvimento
HIV-1/isolamento & purificação
Bocavirus Humano/crescimento & desenvolvimento
Bocavirus Humano/isolamento & purificação
Seres Humanos
Lactente
Masculino
Mamastrovirus/crescimento & desenvolvimento
Mamastrovirus/isolamento & purificação
Norovirus/crescimento & desenvolvimento
Norovirus/isolamento & purificação
Infecções por Parvoviridae/imunologia
Infecções por Parvoviridae/virologia
Prevalência
Rotavirus/crescimento & desenvolvimento
Rotavirus/isolamento & purificação
Infecções por Rotavirus/imunologia
Infecções por Rotavirus/virologia
Carga Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170831
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183196


  3 / 605 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28715976
[Au] Autor:Cortez V; Meliopoulos VA; Karlsson EA; Hargest V; Johnson C; Schultz-Cherry S
[Ad] Endereço:Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38105; email: valerie.cortez@stjude.org , victoria.meliopoulos@stjude.org , erik.karlsson@stjude.org , virginia.hargest@stjude.org , cydney.johnson@stjude.org , stacey.schultz-cherry@stjude.org.
[Ti] Título:Astrovirus Biology and Pathogenesis.
[So] Source:Annu Rev Virol;4(1):327-348, 2017 Sep 29.
[Is] ISSN:2327-0578
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Astroviruses are nonenveloped, positive-sense single-stranded RNA viruses that cause gastrointestinal illness. Although a leading cause of pediatric diarrhea, human astroviruses are among the least characterized enteric RNA viruses. However, by using in vitro methods and animal models to characterize virus-host interactions, researchers have discovered several important properties of astroviruses, including the ability of the astrovirus capsid to act as an enterotoxin, disrupting the gut epithelial barrier. Improved animal models are needed to study this phenomenon, along with the pathogenesis of astroviruses, particularly in those strains that can cause extraintestinal disease. Much like for other enteric viruses, the current dogma states that astroviruses infect in a species-specific manner; however, this assumption is being challenged by growing evidence that these viruses have potential to cross species barriers. This review summarizes these remarkable facets of astrovirus biology, highlighting critical steps toward increasing our understanding of this unique enteric pathogen.
[Mh] Termos MeSH primário: Infecções por Astroviridae/virologia
Gastroenterite/virologia
Mamastrovirus/fisiologia
Mamastrovirus/patogenicidade
[Mh] Termos MeSH secundário: Animais
Infecções por Astroviridae/fisiopatologia
Infecções por Astroviridae/veterinária
Capsídeo/metabolismo
Galinhas/virologia
Diarreia/virologia
Modelos Animais de Doenças
Gastroenterite/fisiopatologia
Seres Humanos
Mucosa Intestinal/fisiopatologia
Mucosa Intestinal/virologia
Mamastrovirus/genética
Camundongos
Permeabilidade
Filogenia
Especificidade da Espécie
Suínos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE
[do] DOI:10.1146/annurev-virology-101416-041742


  4 / 605 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28497550
[Au] Autor:Lau P; Cordey S; Brito F; Tirefort D; Petty TJ; Turin L; Guichebaron A; Docquier M; Zdobnov EM; Waldvogel-Abramowski S; Lecompte T; Kaiser L; Preynat-Seauve O
[Ad] Endereço:Department of Medical Specialties, Division of Infectious Diseases and Division of Laboratory Medicine, University Hospitals of Geneva, Geneva.
[Ti] Título:Metagenomics analysis of red blood cell and fresh-frozen plasma units.
[So] Source:Transfusion;57(7):1787-1800, 2017 Jul.
[Is] ISSN:1537-2995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Although the risk of transmitting infectious agents by blood transfusion is dramatically reduced after donor selection, leukoreduction, and laboratory testing, some could still be present in donor's blood. A description of metagenomes in blood products eligible for transfusion represents relevant information to evaluate the risk of pathogen transmission by transfusion. STUDY DESIGN AND METHODS: Detection of viruses, bacteria, and fungi genomes was made by high-throughput sequencing (HTS) of 600 manufactured blood products eligible for transfusion: 300 red blood cell (RBC) and 300 fresh-frozen plasma (FFP) units. RESULTS: Anelloviruses and human pegivirus, frequent in the blood of healthy individuals, were found. Human papillomavirus type 27 and Merkel cell polyomavirus, present on the skin, were also detected. Unexpectedly, astrovirus MLB2 was identified and characterized in a FFP unit. The presence of astrovirus MLB2 was confirmed in donor's blood and corresponded to an asymptomatic acute viremia. Sequences of bacteria and fungi were also detected; they are likely the result of environmental contamination. CONCLUSION: This study demonstrates that HTS is a promising tool for detecting common and less frequent infectious pathogens in blood products.
[Mh] Termos MeSH primário: Eritrócitos/microbiologia
Eritrócitos/virologia
Metagenômica/métodos
Plasma/microbiologia
Plasma/virologia
[Mh] Termos MeSH secundário: Bancos de Sangue
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Mamastrovirus/isolamento & purificação
Análise de Sequência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE
[do] DOI:10.1111/trf.14148


  5 / 605 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28230787
[Au] Autor:Fischer K; Pinho Dos Reis V; Balkema-Buschmann A
[Ad] Endereço:Friedrich Loeffler Institut, Institute of Novel and Emerging Infectious Diseases, Suedufer 10, 17493 Greifswald-Insel Riems, Germany. Kerstin.fischer@fli.de.
[Ti] Título:Bat Astroviruses: Towards Understanding the Transmission Dynamics of a Neglected Virus Family.
[So] Source:Viruses;9(2), 2017 Feb 21.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Bats belong to the order Chiroptera that represents the second largest order of mammals with more than 1200 species and an almost global distribution. Environmental changes and deforestation have severely influenced many ecosystems, intensifying the contact between wildlife and humans. In recent years, bats have been found to harbor a number of different viruses with zoonotic potential, as well as a great diversity of astroviruses, for which the question of zoonotic potential remains unanswered to date. Human astroviruses have been identified as the causative agent for diarrhea in children and immunocompromised patients. For a long time, astroviruses have been considered to be strictly species-specific. However, a great genetic diversity has recently been discovered among animal and human astroviruses that might indicate the potential of these viruses to cross species barriers. Furthermore, our knowledge about the tissue tropism of astroviruses has been expanded to some neurotropic strains that have recently been shown to be responsible for encephalitis in humans and livestock. This review gives an overview on what is known about astroviruses in bats, humans and livestock, especially bovines and pigs. Future research activities are suggested to unravel astrovirus infection dynamics in bat populations to further assess the zoonotic potential of these viruses.
[Mh] Termos MeSH primário: Infecções por Astroviridae/transmissão
Infecções por Astroviridae/veterinária
Quirópteros/virologia
Mamastrovirus/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Infecções por Astroviridae/virologia
Seres Humanos
Gado
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170224
[St] Status:MEDLINE


  6 / 605 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28224712
[Au] Autor:Pfaff F; Schlottau K; Scholes S; Courtenay A; Hoffmann B; Höper D; Beer M
[Ad] Endereço:Friedrich-Loeffler-Institut, Greifswald - Insel Riems, Germany.
[Ti] Título:A novel astrovirus associated with encephalitis and ganglionitis in domestic sheep.
[So] Source:Transbound Emerg Dis;64(3):677-682, 2017 Jun.
[Is] ISSN:1865-1682
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In June 2013, a 4-year-old Welsh Mountain ewe and in March 2014 a 10-day-old lamb of the same breed and the same flock presented progressive neurological signs including depressed sensorium, tremor, and unusual behaviour. Neuropathological examination of the brain and spinal cord detected non-suppurative polioencephalomyelitis and dorsal root ganglionitis, characteristic of a neurotropic viral agent in both sheep. Metagenomic analysis of different tissue samples from both animals identified a novel Ovine Astrovirus (OvAstV). The presence of viral genome in the central nervous system was confirmed by RT-qPCR. Although the cases presented nine months apart, the identified OvAstV shared nearly identical sequences, differing in only three nucleotide positions across the complete genome. Phylogenetic analysis revealed a close relation of OvAstV to neurotropic bovine astroviruses and an enteric OvAstV. In conclusion, these are the first reported cases of astrovirus infection in domestic sheep that were associated with encephalitis and ganglionitis.
[Mh] Termos MeSH primário: Infecções por Astroviridae/veterinária
Encefalite Viral/veterinária
Mamastrovirus
Doenças dos Ovinos/virologia
[Mh] Termos MeSH secundário: Animais
Infecções por Astroviridae/patologia
Infecções por Astroviridae/virologia
Encéfalo/virologia
Encefalite Viral/virologia
Feminino
Genoma Viral
Mamastrovirus/genética
Metagenômica
Filogenia
Ovinos
Doenças dos Ovinos/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170223
[St] Status:MEDLINE
[do] DOI:10.1111/tbed.12623


  7 / 605 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28218712
[Au] Autor:Vu DL; Bosch A; Pintó RM; Guix S
[Ad] Endereço:Enteric Virus Laboratory, Department of Genetics, Microbiology and Statistics, University of Barcelona, Barcelona 08028, Spain. Diem-Lan.Vu@hcuge.ch.
[Ti] Título:Epidemiology of Classic and Novel Human Astrovirus: Gastroenteritis and Beyond.
[So] Source:Viruses;9(2), 2017 Feb 18.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Since they were identified in 1975, human astroviruses have been considered one of the most important agents of viral acute gastroenteritis in children. However, highly divergent astroviruses infecting humans have been recently discovered and associated with extra-intestinal infections. The report of cases of fatal meningitis and encephalitis, especially in immunocompromised individuals, has broadened their disease spectrum. Although zoonotic transmission among animal and human astroviruses has not been clearly recognized, the genetic similarity between some human and animal viruses makes it likely to occur. This review provides an update on the epidemiology of both classic and novel human astroviruses, and a comprehensive view on confirmed or potential association between astrovirus and human disease.
[Mh] Termos MeSH primário: Gastroenterite/virologia
Mamastrovirus/classificação
Mamastrovirus/isolamento & purificação
[Mh] Termos MeSH secundário: Encefalite/epidemiologia
Encefalite/virologia
Gastroenterite/epidemiologia
Seres Humanos
Meningite/epidemiologia
Meningite/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE


  8 / 605 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28189887
[Au] Autor:Ito M; Kuroda M; Masuda T; Akagami M; Haga K; Tsuchiaka S; Kishimoto M; Naoi Y; Sano K; Omatsu T; Katayama Y; Oba M; Aoki H; Ichimaru T; Mukono I; Ouchi Y; Yamasato H; Shirai J; Katayama K; Mizutani T; Nagai M
[Ad] Endereço:Ishikawa Nanbu Livestock Hygiene Service Center, Kanazawa, Ishikawa 920-3101, Japan.
[Ti] Título:Whole genome analysis of porcine astroviruses detected in Japanese pigs reveals genetic diversity and possible intra-genotypic recombination.
[So] Source:Infect Genet Evol;50:38-48, 2017 Jun.
[Is] ISSN:1567-7257
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Porcine astroviruses (PoAstVs) are ubiquitous enteric virus of pigs that are distributed in several countries throughout the world. Since PoAstVs are detected in apparent healthy pigs, the clinical significance of infection is unknown. However, AstVs have recently been associated with a severe neurological disorder in animals, including humans, and zoonotic potential has been suggested. To date, little is known about the epidemiology of PoAstVs among the pig population in Japan. In this report, we present an analysis of nearly complete genomes of 36 PoAstVs detected by a metagenomics approach in the feces of Japanese pigs. Based on a phylogenetic analysis and pairwise sequence comparison, 10, 5, 15, and 6 sequences were classified as PoAstV2, PoAstV3, PoAstV4, and PoAstV5, respectively. Co-infection with two or three strains was found in individual fecal samples from eight pigs. The phylogenetic trees of ORF1a, ORF1b, and ORF2 of PoAstV2 and PoAstV4 showed differences in their topologies. The PoAstV3 and PoAstV5 strains shared high sequence identities within each genotype in all ORFs; however, one PoAstV3 strain and one PoAstV5 strain showed considerable sequence divergence from the other PoAstV3 and PoAstV5 strains, respectively, in ORF2. Recombination analysis using whole genomes revealed evidence of multiple possible intra-genotype recombination events in PoAstV2 and PoAstV4, suggesting that recombination might have contributed to the genetic diversity and played an important role in the evolution of Japanese PoAstVs.
[Mh] Termos MeSH primário: Infecções por Astroviridae/veterinária
Diarreia/veterinária
Genoma Viral
Mamastrovirus/genética
Filogenia
Doenças dos Suínos/virologia
Proteínas Virais/genética
[Mh] Termos MeSH secundário: Animais
Infecções por Astroviridae/virologia
Evolução Biológica
Diarreia/virologia
Fezes/virologia
Variação Genética
Genótipo
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Japão
Mamastrovirus/classificação
Fases de Leitura Aberta
Recombinação Genética
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170213
[St] Status:MEDLINE


  9 / 605 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28106836
[Au] Autor:Arias CF; DuBois RM
[Ad] Endereço:Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62210, Mexico. arias@ibt.unam.mx.
[Ti] Título:The Astrovirus Capsid: A Review.
[So] Source:Viruses;9(1), 2017 Jan 19.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Astroviruses are enterically transmitted viruses that cause infections in mammalian and avian species. Astroviruses are nonenveloped, icosahedral viruses comprised of a capsid protein shell and a positive-sense, single-stranded RNA genome. The capsid protein undergoes dramatic proteolytic processing both inside and outside of the host cell, resulting in a coordinated maturation process that affects cellular localization, virus structure, and infectivity. After maturation, the capsid protein controls the initial phases of virus infection, including virus attachment, endocytosis, and genome release into the host cell. The astrovirus capsid is the target of host antibodies including virus-neutralizing antibodies. The capsid protein also mediates the binding of host complement proteins and inhibits complement activation. Here, we will review our knowledge on the astrovirus capsid protein (CP), with particular attention to the recent structural, biochemical, and virological studies that have advanced our understanding of the astrovirus life cycle.
[Mh] Termos MeSH primário: Infecções por Astroviridae/virologia
Proteínas do Capsídeo/metabolismo
Mamastrovirus/metabolismo
[Mh] Termos MeSH secundário: Animais
Capsídeo/metabolismo
Proteínas do Capsídeo/genética
Seres Humanos
Mamastrovirus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Capsid Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170508
[Lr] Data última revisão:
170508
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170121
[St] Status:MEDLINE


  10 / 605 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28040514
[Au] Autor:Bennett S; Gunson RN
[Ad] Endereço:West of Scotland Specialist Virology Centre, United Kingdom. Electronic address: susan.bennett@ggc.scot.nhs.uk.
[Ti] Título:The development of a multiplex real-time RT-PCR for the detection of adenovirus, astrovirus, rotavirus and sapovirus from stool samples.
[So] Source:J Virol Methods;242:30-34, 2017 Apr.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Viral gastroenteritis is a major health problem with significant morbidity and economic consequences. Viral gastroenteritis is caused by a number of viruses, including norovirus, rotavirus, adenovirus, astrovirus, and sapovirus. Conventional diagnosis is based on direct antigen detection and electron microscopy, however enzyme immunoassay's are insensitive and not available for all relevant pathogens, and electron microscope (EM) is no longer routinely carried out in most laboratories. Most laboratories now offer norovirus real-time PCR testing however the availability of other assays is variable. Commercial methods for the detection of inflectional intestinal disease (IID) are available but these can be expensive and are not commonly used. This paper describes the development of a single multiplex assay for the simultaneous detection of adenovirus, astrovirus, rotavirus and sapovirus from stool samples. The multiplex was evaluated by assessing endpoint sensitivity, specificity, panel of clinical samples, quality control (QC) panel and the robustness and reproducibility of the multiplex.
[Mh] Termos MeSH primário: Adenovírus Humanos/isolamento & purificação
Caliciviridae/isolamento & purificação
Fezes/virologia
Gastroenterite/diagnóstico
Mamastrovirus/isolamento & purificação
Reação em Cadeia da Polimerase Multiplex/métodos
Rotavirus/isolamento & purificação
[Mh] Termos MeSH secundário: Infecções por Adenovirus Humanos/diagnóstico
Infecções por Adenovirus Humanos/virologia
Adenovírus Humanos/genética
Caliciviridae/genética
Infecções por Caliciviridae/diagnóstico
Infecções por Caliciviridae/virologia
Primers do DNA
Sondas de DNA
Gastroenterite/virologia
Seres Humanos
Técnicas Imunoenzimáticas
Limite de Detecção
Mamastrovirus/genética
Norovirus/genética
Norovirus/isolamento & purificação
Reação em Cadeia da Polimerase em Tempo Real
Reprodutibilidade dos Testes
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Rotavirus/genética
Infecções por Rotavirus/diagnóstico
Infecções por Rotavirus/virologia
Sapovirus/genética
Sapovirus/isolamento & purificação
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA Probes)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170102
[St] Status:MEDLINE



página 1 de 61 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde