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  1 / 437 MEDLINE  
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[PMID]:28843856
[Au] Autor:Chen QZ; Luo F; Lu MX; Li N; Teng Y; Huang QL; Zhu N; Wang GY; Yue M; Zhang Y; Feng Y; Xiong HR; Hou W
[Ad] Endereço:State Key Laboratory of Virology, Institute of Medical Virology, Hubei Province Key Laboratory of Allergy and Immunology, School of Basic Medical Sciences, Wuhan University, 185 Donghu Road, Wuhan 430071, Hubei Province, China.
[Ti] Título:HTNV-induced upregulation of miR-146a in HUVECs promotes viral infection by modulating pro-inflammatory cytokine release.
[So] Source:Biochem Biophys Res Commun;493(1):807-813, 2017 Nov 04.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Increasing research has shown a link between viruses and miRNAs, such as miRNA-146a, in regulating virus infection and replication. In the current study, the association between miR-146a and hantaan virus (HTNV) infection in human umbilical vein endothelial cells (HUVECs) was investigated, with a focus on examining the expression of pro-inflammatory cytokines. The results showed that HTNV infection promoted the production of miR-146a in HUVECs and activated nuclear factor-κB (NF-κB) signaling, along with the upregulation of pro-inflammatory cytokines, including interleukin 8 (IL-8), C-C Motif Chemokine Ligand 5 (CCL5, also RANTES), interferon-inducible protein-10 (IP-10) and interferon beta (IFN-ß). Moreover, miR-146a exhibited a negative regulatory effect on the NF-κB pathway. Accordingly, a miR-146a inhibitor increased the expression of IL-8, CCL5, IP-10 and IFN-ß, whereas a miR-146a mimic reduced the levels of these cytokines. Consequently, exogenous transduction of miR-146a significantly enhanced HTNV replication in HUVEC cells. We also discovered that viral proteins (NP/GP) contributed to miR-146a expression via enhancement the activity of miR-146a promoter. In conclusion, these results imply the negative regulation of miR-146a on the production of HTNV-induced pro-inflammatory cytokines contributes to virus replication, which suggest that miR-146a may be regarded as a novel therapeutic target for HTNV infection.
[Mh] Termos MeSH primário: Citocinas/imunologia
Células Endoteliais/imunologia
Células Endoteliais/virologia
Vírus Hantaan/imunologia
Febre Hemorrágica com Síndrome Renal/imunologia
MicroRNAs/imunologia
Internalização do Vírus
[Mh] Termos MeSH secundário: Células Cultivadas
Febre Hemorrágica com Síndrome Renal/patologia
Febre Hemorrágica com Síndrome Renal/virologia
Seres Humanos
Mediadores da Inflamação/imunologia
Regulação para Cima/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Inflammation Mediators); 0 (MIRN146 microRNA, human); 0 (MicroRNAs)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170828
[St] Status:MEDLINE


  2 / 437 MEDLINE  
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[PMID]:28814703
[Au] Autor:Shin M
[Ad] Endereço:Dept. of Science Studies, Chonbuk National University, Jeonju-si, Jeollabuk-do, KOREA.
[Ti] Título:Becoming an International Scientist in South Korea: Ho Wang Lee's Research Activity about Epidemic Hemorrhagic Fever.
[So] Source:Uisahak;26(1):95-124, 2017 Apr.
[Is] ISSN:1225-505X
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:In the 1960-70s, South Korea was still in the position of a science latecomer. Although the scientific research environment in South Korea at that time was insufficient, there was a scientist who achieved outcomes that could be recognized internationally while acting in South Korea. He was Ho Wang Lee(1928~ ) who found Hantann Virus that causes epidemic hemorrhagic fever for the first time in the world. It became a clue to identify causative viruses of hemorrhagic diseases that were scattered here and there throughout the world. In addition, these outcomes put Ho Wang Lee on the global center of research into epidemic hemorrhagic fever. This paper examines how a Korean scientist who was in the periphery of virology could go into the central area of virology. Also this article shows the process through which the virus found by Ho Wang Lee was registered with the international academia and he proceeded with follow-up research based on this progress to reach the level at which he generalized epidemic hemorrhagic fever related studies throughout the world. While he was conducting the studies, experimental methods that he had never experienced encountered him as new difficulties. He tried to solve the new difficulties faced in his changed status through devices of cooperation and connection. Ho Wang Lee's growth as a researcher can be seen as well as a view of a researcher that grew from a regional level to an international level and could advance from the area of non-mainstream into the mainstream. This analytic tool is meaningful in that it can be another method of examining the growth process of scientists in South Korea or developing countries.
[Mh] Termos MeSH primário: Vírus Hantaan/fisiologia
Febre Hemorrágica com Síndrome Renal/história
Virologia/história
[Mh] Termos MeSH secundário: Febre Hemorrágica com Síndrome Renal/virologia
História do Século XX
História do Século XXI
República da Coreia
[Pt] Tipo de publicação:BIOGRAPHY; HISTORICAL ARTICLE; JOURNAL ARTICLE; PORTRAITS
[Ps] Nome de pessoa como assunto:Lee HW
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:QIS
[Da] Data de entrada para processamento:170818
[St] Status:MEDLINE
[do] DOI:10.13081/kjmh.2017.26.95


  3 / 437 MEDLINE  
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[PMID]:28685549
[Au] Autor:Tang Z; Xu XJ; He XJ; Liang ZS; Liang WB; Li Y; Gao K
[Ad] Endereço:School of Information Management, Guangxi Medical University, Nanning, Guangxi, China.
[Ti] Título:Analysis of hemorrhagic fever with renal syndrome and its pathogenic gene sequence based on geographic information system.
[So] Source:J Biol Regul Homeost Agents;31(2):431-438, 2017 Apr-Jun.
[Is] ISSN:0393-974X
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:This study analyzed the temporal-spatial distribution characteristics, epidemiological characteristics and gene sequences of hemorrhagic fever with renal syndrome (HFRS) in Guangxi, with the intention of providing a theoretical and technical support for the prevention of HFRS. A map of the incidence of HFRS of different cities in Guangxi was drawn up using the Geographic Information System (GIS) to investigate the epidemiological characteristics and infection source of HFRS between 2013 and 2016. Guangxi has a low incidence of HFRS, and autumn and winter are the main high-incidence seasons. Cases of HFRS were reported in all regions in Guangxi except Laibin city between 2013 and 2016. The distribution of cases in the four years suggested that Guilin, Nanning, Hechi and Wuzhou were the main infected regions, especially the local areas in the north of Guilin. The nucleotide and amino acid of S fragment and M fragment of Hantaviruses (HV) detected were highly homologous, and no obvious variation was found. Through analyzing the space-time characteristics, epidemiological characteristics and gene sequence of HFRS in Guangxi, it was found that areas rich in water, grass and moisture, such as paddy fields, are the main active areas for the host of HFRS.
[Mh] Termos MeSH primário: Genes Virais
Sistemas de Informação Geográfica
Vírus Hantaan
Febre Hemorrágica com Síndrome Renal
Reforma Urbana
[Mh] Termos MeSH secundário: Animais
China/epidemiologia
Feminino
Vírus Hantaan/genética
Vírus Hantaan/isolamento & purificação
Vírus Hantaan/patogenicidade
Febre Hemorrágica com Síndrome Renal/epidemiologia
Febre Hemorrágica com Síndrome Renal/genética
Seres Humanos
Masculino
Camundongos
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170708
[St] Status:MEDLINE


  4 / 437 MEDLINE  
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[PMID]:28202761
[Au] Autor:Ma H; Han P; Ye W; Chen H; Zheng X; Cheng L; Zhang L; Yu L; Wu X; Xu Z; Lei Y; Zhang F
[Ad] Endereço:Department of Microbiology, Fourth Military Medical University, Xi'an, China.
[Ti] Título:The Long Noncoding RNA NEAT1 Exerts Antihantaviral Effects by Acting as Positive Feedback for RIG-I Signaling.
[So] Source:J Virol;91(9), 2017 May 01.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hantavirus infection, which causes zoonotic diseases with a high mortality rate in humans, has long been a global public health concern. Over the past decades, accumulating evidence suggests that long noncoding RNAs (lncRNAs) play key regulatory roles in innate immunity. However, the involvement of host lncRNAs in hantaviral control remains uncharacterized. In this study, we identified the lncRNA NEAT1 as a vital antiviral modulator. NEAT1 was dramatically upregulated after Hantaan virus (HTNV) infection, whereas its downregulation or delayed host innate immune responses and aggravated HTNV replication. Ectopic expression of NEAT1 enhanced beta interferon (IFN-ß) production and suppressed HTNV infection. Further investigation suggested that NEAT1 served as positive feedback for RIG-I signaling. HTNV infection activated NEAT1 transcription through the RIG-I-IRF7 pathway, whereas NEAT1 removed the transcriptional inhibitory effects of the splicing factor proline- and glutamine-rich protein (SFPQ) by relocating SFPQ to paraspeckles, thus promoting the expression of RIG-I and DDX60. RIG-I and DDX60 had synergic effects on IFN production. Taken together, our findings demonstrate that NEAT1 modulates the innate immune response against HTNV infection, providing another layer of information about the role of lncRNAs in controlling viral infections. Hantaviruses have attracted worldwide attention as archetypal emerging pathogens. Recently, increasing evidence has highlighted long noncoding RNAs (lncRNAs) as key regulators of innate immunity; however, their roles in hantavirus infection remain unknown. In the present work, a new unexplored function of lncRNA NEAT1 in controlling HTNV replication was found. NEAT1 promoted interferon (IFN) responses by acting as positive feedback for RIG-I signaling. This lncRNA was induced by HTNV through the RIG-I-IRF7 pathway in a time- and dose-dependent manner and promoted HTNV-induced IFN production by facilitating RIG-I and DDX60 expression. Intriguingly, NEAT1 relocated SFPQ and formed paraspeckles after HTNV infection, which might reverse inhibitive effects of SFPQ on the transcription of RIG-I and DDX60. To the best of our knowledge, this is the first study to address the regulatory role of the lncRNA NEAT1 in host innate immunity after HTNV infection. In summary, our findings provide additional insights regarding the role of lncRNAs in controlling viral infections.
[Mh] Termos MeSH primário: Proteína DEAD-box 58/metabolismo
Vírus Hantaan/genética
Vírus Hantaan/imunologia
Infecções por Hantavirus/imunologia
Imunidade Inata/genética
RNA Longo não Codificante/genética
[Mh] Termos MeSH secundário: Células A549
Animais
Linhagem Celular Tumoral
Cercopithecus aethiops
RNA Helicases DEAD-box/metabolismo
Células HEK293
Vírus Hantaan/crescimento & desenvolvimento
Infecções por Hantavirus/virologia
Células HeLa
Células Endoteliais da Veia Umbilical Humana
Seres Humanos
Interferon beta/biossíntese
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Fator de Processamento Associado a PTB/metabolismo
Interferência de RNA
RNA Longo não Codificante/biossíntese
RNA Interferente Pequeno/genética
Transdução de Sinais/genética
Células Vero
Replicação Viral/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (NEAT1 long non-coding RNA, human); 0 (PTB-Associated Splicing Factor); 0 (RNA, Long Noncoding); 0 (RNA, Small Interfering); 77238-31-4 (Interferon-beta); EC 3.6.1.- (DDX58 protein, human); EC 3.6.1.- (DDX60 protein, human); EC 3.6.4.13 (DEAD Box Protein 58); EC 3.6.4.13 (DEAD-box RNA Helicases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170217
[St] Status:MEDLINE


  5 / 437 MEDLINE  
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[PMID]:28141833
[Au] Autor:Tian H; Yu P; Bjørnstad ON; Cazelles B; Yang J; Tan H; Huang S; Cui Y; Dong L; Ma C; Ma C; Zhou S; Laine M; Wu X; Zhang Y; Wang J; Yang R; Stenseth NC; Xu B
[Ad] Endereço:State Key Laboratory of Remote Sensing Science, College of Global Change and Earth System Science, Beijing Normal University, Beijing, China.
[Ti] Título:Anthropogenically driven environmental changes shift the ecological dynamics of hemorrhagic fever with renal syndrome.
[So] Source:PLoS Pathog;13(1):e1006198, 2017 Jan.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Zoonoses are increasingly recognized as an important burden on global public health in the 21st century. High-resolution, long-term field studies are critical for assessing both the baseline and future risk scenarios in a world of rapid changes. We have used a three-decade-long field study on hantavirus, a rodent-borne zoonotic pathogen distributed worldwide, coupled with epidemiological data from an endemic area of China, and show that the shift in the ecological dynamics of Hantaan virus was closely linked to environmental fluctuations at the human-wildlife interface. We reveal that environmental forcing, especially rainfall and resource availability, exert important cascading effects on intra-annual variability in the wildlife reservoir dynamics, leading to epidemics that shift between stable and chaotic regimes. Our models demonstrate that bimodal seasonal epidemics result from a powerful seasonality in transmission, generated from interlocking cycles of agricultural phenology and rodent behavior driven by the rainy seasons.
[Mh] Termos MeSH primário: Vírus Hantaan/fisiologia
Febre Hemorrágica com Síndrome Renal/epidemiologia
Interações Hospedeiro-Patógeno
Zoonoses/epidemiologia
[Mh] Termos MeSH secundário: Animais
Teorema de Bayes
China/epidemiologia
Ecologia
Meio Ambiente
Feminino
Geografia
Febre Hemorrágica com Síndrome Renal/transmissão
Febre Hemorrágica com Síndrome Renal/virologia
Seres Humanos
Filogenia
Gravidez
Chuvas
Risco
Roedores
Estações do Ano
Zoonoses/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006198


  6 / 437 MEDLINE  
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[PMID]:28122569
[Au] Autor:Shimizu K; Koma T; Yoshimatsu K; Tsuda Y; Isegawa Y; Arikawa J
[Ad] Endereço:Department of Microbiology, Hokkaido University Graduate School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo, 060-8638, Japan. kshimizu@med.hokudai.ac.jp.
[Ti] Título:Appearance of renal hemorrhage in adult mice after inoculation of patient-derived hantavirus.
[So] Source:Virol J;14(1):13, 2017 Jan 26.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Hemorrhagic fever with renal syndrome (HFRS) caused by hantavirus infection is characterized by fever, renal dysfunction and hemorrhage. An animal model mimicking symptoms of HFRS remains to be established. In this study, we evaluated the pathogenicity of an HFRS patient-derived Hantaan virus (HTNV) in adult mice. METHODS: Five clones of HTNV strain KHF 83-61 BL (KHFV) that was derived from blood of an HFRS patient were obtained by plaque cloning. The pathogenicity of the virus clones was evaluated by using 6-week-old female BALB/c mice. Sequence analysis of the viral genome was performed by conventional methods. RESULTS: All of the mice intravenously inoculated with KHFV clone (cl)-1, -2, -3 and -5 showed signs of disease such as transient body weight loss, ruffled fur, reduced activity and remarkably prominent hemorrhage in the renal medulla at 6 to 9 days post-inoculation (dpi) and then recovered. In contrast, mice intravenously inoculated with KHFV cl-4 did not show any signs of disease. We selected KHFV cl-5 and cl-4 as representative of high-pathogenic and low-pathogenic clones, respectively. Quantities of viral RNA in kidneys of KHFV cl-5-infected mice were larger than those in KHFV cl-4-infected mice at any time point examined (3, 6, 9 and 12 dpi). The quantities of viral RNA of KHFV cl-5 and cl-4 peaked at 3 dpi, which was before the onset of disease. Sequence analysis revealed that the amino acid at position 417 in the glycoprotein Gn was the sole difference in viral proteins between KHFV cl-5 and cl-4. The result suggests that amino acid at position 417 in Gn is related to the difference in pathogenicity between KHFV cl-5 and cl-4. When the inoculum of KHFV cl-5 was pretreated with a neutralizing antibody against HTNV strain 76-118, which belongs to the same serotype as KHFV clones, mice did not show any signs of disease, confirming that the disease was caused by KHFV infection. CONCLUSION: We found that an HFRS patient-derived HTNV caused renal hemorrhage in adult mice. We anticipate that this infection model will be a valuable tool for understanding the pathogenesis of HFRS.
[Mh] Termos MeSH primário: Modelos Animais de Doenças
Vírus Hantaan/patogenicidade
Hemorragia/patologia
Febre Hemorrágica com Síndrome Renal/patologia
Febre Hemorrágica com Síndrome Renal/virologia
Rim/patologia
[Mh] Termos MeSH secundário: Animais
Feminino
Genoma Viral
Vírus Hantaan/genética
Vírus Hantaan/isolamento & purificação
Seres Humanos
Camundongos Endogâmicos BALB C
Oxalobacteraceae
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170127
[St] Status:MEDLINE
[do] DOI:10.1186/s12985-017-0686-8


  7 / 437 MEDLINE  
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[PMID]:27895275
[Au] Autor:Song DH; Kim WK; Gu SH; Lee D; Kim JA; No JS; Lee SH; Wiley MR; Palacios G; Song JW; Jeong ST
[Ad] Endereço:The 5th R&D Institute, Agency for Defense Development, Daejeon, Republic of Korea.
[Ti] Título:Sequence-Independent, Single-Primer Amplification Next-Generation Sequencing of Hantaan Virus Cell Culture-Based Isolates.
[So] Source:Am J Trop Med Hyg;96(2):389-394, 2017 Feb 08.
[Is] ISSN:1476-1645
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hantaan virus (HTNV), identified in the striped field mouse ( ), belongs to the genus of the family and contains tripartite RNA genomes, small (S), medium (M), and large (L) segments. HTNV is a major causative for hemorrhagic fever with renal syndrome (HFRS) with fatality rates ranging from 1% to 15% in the Republic of Korea (ROK) and China. Defining of HTNV whole-genome sequences and isolation of the infectious particle play a critical role in the characterization and preventive and therapeutic strategies of hantavirus outbreaks. Next-generation sequencing (NGS) provides an advanced tool for massive genomic sequencing of viruses. However, the isolation of viral infectious particles is a huge obstacle to investigate and develop anti-virals for hantaviruses. Here, we report 12 HTNV isolates from lung tissues of the striped field mouse in the highly HFRS-endemic areas. Sequence-independent, single-primer amplification (SISPA) NGS was attempted to recover the genomic sequences of HTNV isolates. The nucleotide sequence of HTNV S, M, and L segments were covered up to 99.4-100%, 97.5-100%, and 95.6-99.8%, respectively, based on the full length of the prototype HTNV 76-118. The whole-genome sequencing of HTNV isolates was accomplished by additional reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification cDNA ends (RACE) PCR. In conclusion, this study will lead to the attempt and usage of SISPA NGS technologies to delineate the whole-genome sequence of hantaviruses, providing a new era of viral genomics for the surveillance, trace, and disease risk management of HFRS incidents.
[Mh] Termos MeSH primário: Variação Genética
Genoma Viral
Vírus Hantaan/genética
Febre Hemorrágica com Síndrome Renal/virologia
Análise de Sequência de DNA
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Primers do DNA
Estudo de Associação Genômica Ampla
Camundongos
Murinae/virologia
Filogenia
República da Coreia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:161130
[St] Status:MEDLINE
[do] DOI:10.4269/ajtmh.16-0683


  8 / 437 MEDLINE  
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[PMID]:27824286
[Au] Autor:Li Z; Zeng H; Wang Y; Zhang Y; Cheng L; Zhang F; Lei Y; Jin B; Ma Y; Chen L
[Ad] Endereço:a Department of Immunology , the Fourth Military Medical University , Xi'an , Shaanxi , China.
[Ti] Título:The assessment of Hantaan virus-specific antibody responses after the immunization program for hemorrhagic fever with renal syndrome in northwest China.
[So] Source:Hum Vaccin Immunother;13(4):802-807, 2017 Apr 03.
[Is] ISSN:2164-554X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Xianyang city is one of the main hemorrhagic fever with renal syndrome (HFRS) epidemic areas in northwest China. Although the HFRS immunity program has been provided in this city, HFRS is still occurred every year. In order to implement the vaccination program effectively and to control HFRS, the analysis of antibody responses specific to Hantaan virus (HTNV) in individuals after vaccination is essential. In this study, a total of 100 subjects were divided into 5 groups: unvaccinated, 1, 3, 29 and 33 months after boost vaccination. The levels and the positive rates of HTNV-NP-specific IgM and IgG antibodies as well as HTNV neutralizing antibodies were significantly increased in the serum of the vaccinated individuals. The positive rates and levels of HTNV-NP-specific IgG and HTNV neutralizing antibody reached their highest values at 3 months respectively and could be sustained up to 33 months after vaccination. Moreover, the titres of HTNV-NP-specific IgM or IgG antibody and the titres of HTNV neutralizing antibody at 1 month after vaccination have a positive correlation. The level of HTNV-NP-specific IgG antibody was much higher than that of HTNV-NP-specific IgM antibody or HTNV neutralizing antibody. In addition, the strongest responses of antibody-secreting cells were observed at 3 months after vaccination, which was consistent with the serum results. Therefore, the HFRS immunization program is effective to induce humoral immunity in the population of northwest China.
[Mh] Termos MeSH primário: Anticorpos Antivirais/sangue
Vírus Hantaan/imunologia
Febre Hemorrágica com Síndrome Renal/prevenção & controle
Programas de Imunização
Vacinas Virais/imunologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Anticorpos Neutralizantes/sangue
Formação de Anticorpos
China
Feminino
Seres Humanos
Imunoglobulina G/sangue
Imunoglobulina M/sangue
Masculino
Meia-Idade
Fatores de Tempo
Vacinas Virais/administração & dosagem
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Neutralizing); 0 (Antibodies, Viral); 0 (Immunoglobulin G); 0 (Immunoglobulin M); 0 (Viral Vaccines)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161109
[St] Status:MEDLINE
[do] DOI:10.1080/21645515.2016.1253645


  9 / 437 MEDLINE  
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[PMID]:27301695
[Au] Autor:Jiang W; Ma N; Hui Q; Chen B; Qiu Y; Sun H; Li Y
[Ad] Endereço:Changchun Institute of Biological Products Co. Ltd., Changchun, P.R. China.
[Ti] Título:Type inactivated bivalent hantavirus vaccines using dual fluorescence quantitative real-time PCR: Establishment and evaluation.
[So] Source:J Med Virol;89(1):10-16, 2017 Jan.
[Is] ISSN:1096-9071
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To establish a dual fluorescence quantitative real-time polymerase chain reaction (DFqRT-PCR) method for typing inactivated bivalent vaccines of Hantaan virus (HTNV, type I) and Seoul virus (SEOV, type II) found in China. Primers and probes were designed based on S segment of the Hantavirus. FAM and HEX were added to the probes for the type I and type II vaccines, respectively. The S fragments were cloned into the pMD18-T vector to create pMD18-T/HTNV and pMD18-T/SEOV plasmids, respectively. The DFqRT-PCR reaction conditions were optimized. The specificity, sensitivity, accuracy, and reproducibility of the DFqRT-PCR method within and among batches of the samples and labs were evaluated. The sensitivity of the DFqRT-PCR method for detecting type I and II vaccines was 4.68 × 10 copies/µL and 5 × 10 copies/µL of pMD18-T/HTNV and pMD18-T/SEOV, respectively. This method distinguished the hantavirus vaccines from several other virus genus and distinguished the type I and II vaccines from each other with coefficients of variation (CVs) of the intra- and inter-batch assay of 0.19-0.66% and 0.09-0.69%, respectively. For bivalent inactivated vaccine, the CVs of the intra- and inter-batch assays, and the intra- and inter-laboratory assays were 0.06-1.82% for the samples from the same batches and different batches from the same manufacturer or the samples from the different manufacturers. The DFqRT-PCR method was simple, fast, and sensitive for identifying and determining the inactivated hantavirus vaccine with high accuracy and reproducibility. Therefore, it can be used for quality control of hantavirus vaccines. J. Med. Virol. 89:10-16, 2017. © 2016 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Vírus Hantaan/genética
Reação em Cadeia da Polimerase em Tempo Real/métodos
Tecnologia Farmacêutica/métodos
Vacinas Virais/genética
[Mh] Termos MeSH secundário: China
Fluorescência
Seres Humanos
Controle de Qualidade
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Vacinas de Produtos Inativados/genética
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Vaccines, Inactivated); 0 (Viral Vaccines)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160616
[St] Status:MEDLINE
[do] DOI:10.1002/jmv.24599


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[PMID]:28066721
[Au] Autor:Cheng LF; Wang F; Zhang L; Yu L; Ye W; Liu ZY; Ying QK; Wu XA; Xu ZK; Zhang FL
[Ad] Endereço:Department of Microbiology, Fourth Military Medical University Xi'an, China.
[Ti] Título:Incorporation of GM-CSF or CD40L Enhances the Immunogenicity of Hantaan Virus-Like Particles.
[So] Source:Front Cell Infect Microbiol;6:185, 2016.
[Is] ISSN:2235-2988
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:A safe and effective Hantaan virus (HTNV) vaccine is highly desirable because HTNV causes an acute and often fatal disease (hemorrhagic fever with renal syndrome, HFRS). Since the immunity of the inactivated vaccine is weak and the safety is poor, HTNV virus-like particles (VLPs) offer an attractive and safe alternative. These particles lack the viral genome but are perceived by the immune system as virus particles. We hypothesized that adding immunostimulatory signals to VLPs would enhance their efficacy. To accomplish this enhancement, we generated chimeric HTNV VLPs containing glycosylphosphatidylinositol (GPI)-anchored granulocyte macrophage colony-stimulating factor (GM-CSF) or CD40 ligand (CD40L) and investigated their biological activity . The immunization of mice with chimeric HTNV VLPs containing GM-CSF or CD40L induced stronger humoral immune responses and cellular immune responses compared to the HTNV VLPs and Chinese commercial inactivated hantavirus vaccine. Chimeric HTNV VLPs containing GM-CSF or CD40L also protected mice from an HTNV challenge. Altogether, our results suggest that anchoring immunostimulatory molecules into HTNV VLPs can be a potential approach for the control and prevention of HFRS.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/administração & dosagem
Ligante de CD40/administração & dosagem
Fator Estimulador de Colônias de Granulócitos e Macrófagos/administração & dosagem
Vírus Hantaan/imunologia
Vacinas de Partículas Semelhantes a Vírus/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/sangue
Ligante de CD40/genética
Modelos Animais de Doenças
Fator Estimulador de Colônias de Granulócitos e Macrófagos/genética
Febre Hemorrágica com Síndrome Renal/prevenção & controle
Leucócitos Mononucleares/imunologia
Camundongos
Vacinas Sintéticas/administração & dosagem
Vacinas Sintéticas/genética
Vacinas Sintéticas/imunologia
Vacinas de Partículas Semelhantes a Vírus/administração & dosagem
Vacinas de Partículas Semelhantes a Vírus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Antibodies, Viral); 0 (Vaccines, Synthetic); 0 (Vaccines, Virus-Like Particle); 147205-72-9 (CD40 Ligand); 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170110
[St] Status:MEDLINE
[do] DOI:10.3389/fcimb.2016.00185



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