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  1 / 11 MEDLINE  
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[PMID]:26647464
[Au] Autor:M D B; B H
[Ti] Título:Nairobi sheep disease virus/Ganjam virus.
[So] Source:Rev Sci Tech;34(2):411-7, 2015 Aug.
[Is] ISSN:0253-1933
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Nairobi sheep disease virus (NSDV) is a tick-borne virus which causes a severe disease in sheep and goats, and has been responsible for several outbreaks of disease in East Africa. The virus is also found in the Indian subcontinent, where it is known as Ganjam virus. The virus only spreads through the feeding of competent infected ticks, and is therefore limited in its geographic distribution by the distribution of those ticks, Rhipicephalus appendiculata in Africa and Haemaphysalis intermedia in India. Animals bred in endemic areas do not normally develop disease, and the impact is therefore primarily on animals being moved for trade or breeding purposes. The disease caused by NSDV has similarities to several other ruminant diseases, and laboratory diagnosis is necessary for confirmation. There are published methods for diagnosis based on polymerase chain reaction, for virus growth in cell culture and for other simple diagnostic tests, though none has been commercialised. There is no established vaccine against NSDV, although cell-culture attenuated strains have been developed which show promise and could be put into field trials if it were deemed necessary. The virus is closely related to Crimean-Congo haemorrhagic fever virus, and studies on NSDV may therefore be useful in understanding this important human pathogen.
[Mh] Termos MeSH primário: Doença dos Ovinos de Nairobi/virologia
Vírus da Doença do Carneiro de Nairobi/genética
[Mh] Termos MeSH secundário: África/epidemiologia
Animais
Índia/epidemiologia
Doença dos Ovinos de Nairobi/epidemiologia
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1512
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151210
[St] Status:MEDLINE


  2 / 11 MEDLINE  
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[PMID]:25905707
[Au] Autor:Lasecka L; Bin-Tarif A; Bridgen A; Juleff N; Waters RA; Baron MD
[Ad] Endereço:The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, United Kingdom.
[Ti] Título:Antibodies to the core proteins of Nairobi sheep disease virus/Ganjam virus reveal details of the distribution of the proteins in infected cells and tissues.
[So] Source:PLoS One;10(4):e0124966, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nairobi sheep disease virus (NSDV; also called Ganjam virus in India) is a bunyavirus of the genus Nairovirus. It causes a haemorrhagic gastroenteritis in sheep and goats with mortality up to 90%. The virus is closely related to the human pathogen Crimean-Congo haemorrhagic fever virus (CCHFV). Little is currently known about the biology of NSDV. We have generated specific antibodies against the virus nucleocapsid protein (N) and polymerase (L) and used these to characterise NSDV in infected cells and to study its distribution during infection in a natural host. Due to its large size and the presence of a papain-like protease (the OTU-like domain) it has been suggested that the L protein of nairoviruses undergoes an autoproteolytic cleavage into polymerase and one or more accessory proteins. Specific antibodies which recognise either the N-terminus or the C-terminus of the NSDV L protein showed no evidence of L protein cleavage in NSDV-infected cells. Using the specific anti-N and anti-L antibodies, it was found that these viral proteins do not fully colocalise in infected cells; the N protein accumulated near the Golgi at early stages of infection while the L protein was distributed throughout the cytoplasm, further supporting the multifunctional nature of the L protein. These antibodies also allowed us to gain information about the organs and cell types targeted by the virus in vivo. We could detect NSDV in cryosections prepared from various tissues collected post-mortem from experimentally inoculated animals; the virus was found in the mucosal lining of the small and large intestine, in the lungs, and in mesenteric lymph nodes (MLN), where NSDV appeared to target monocytes and/or macrophages.
[Mh] Termos MeSH primário: Anticorpos Antivirais/imunologia
Doença dos Ovinos de Nairobi/imunologia
Vírus da Doença do Carneiro de Nairobi/imunologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Ovinos
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150424
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0124966


  3 / 11 MEDLINE  
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[PMID]:25811222
[Au] Autor:Gong S; He B; Wang Z; Shang L; Wei F; Liu Q; Tu C
[Ti] Título:Nairobi sheep disease virus RNA in ixodid ticks, China, 2013.
[So] Source:Emerg Infect Dis;21(4):718-20, 2015 Apr.
[Is] ISSN:1080-6059
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Ixodidae/virologia
Vírus da Doença do Carneiro de Nairobi/genética
RNA Viral
[Mh] Termos MeSH secundário: Animais
Vetores Aracnídeos/virologia
China
Dados de Sequência Molecular
Vírus da Doença do Carneiro de Nairobi/classificação
Vírus da Doença do Carneiro de Nairobi/isolamento & purificação
Filogenia
[Pt] Tipo de publicação:LETTER; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:150401
[Lr] Data última revisão:
150401
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150327
[St] Status:MEDLINE
[do] DOI:10.3201/eid2104.141602


  4 / 11 MEDLINE  
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[PMID]:24714576
[Au] Autor:Lasecka L; Baron MD
[Ad] Endereço:The Pirbright Institute, Pirbright, Woking, Surrey, United Kingdom.
[Ti] Título:The nairovirus nairobi sheep disease virus/ganjam virus induces the translocation of protein disulphide isomerase-like oxidoreductases from the endoplasmic reticulum to the cell surface and the extracellular space.
[So] Source:PLoS One;9(4):e94656, 2014.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nairobi sheep disease virus (NSDV) of the genus Nairovirus causes a haemorrhagic gastroenteritis in sheep and goats with mortality up to 90%; the virus is found in East and Central Africa, and in India, where the virus is called Ganjam virus. NSDV is closely related to the human pathogen Crimean-Congo haemorrhagic fever virus, which also causes a haemorrhagic disease. As with other nairoviruses, replication of NSDV takes place in the cytoplasm and the new virus particles bud into the Golgi apparatus; however, the effect of viral replication on cellular compartments has not been studied extensively. We have found that the overall structure of the endoplasmic reticulum (ER), the ER-Golgi intermediate compartment and the Golgi were unaffected by infection with NSDV. However, we observed that NSDV infection led to the loss of protein disulphide isomerase (PDI), an oxidoreductase present in the lumen of the endoplasmic reticulum (ER) and which assists during protein folding, from the ER. Further investigation showed that NSDV-infected cells have high levels of PDI at their surface, and PDI is also secreted into the culture medium of infected cells. Another chaperone from the PDI family, ERp57, was found to be similarly affected. Analysis of infected cells and expression of individual viral glycoproteins indicated that the NSDV PreGn glycoprotein is involved in redistribution of these soluble ER oxidoreductases. It has been suggested that extracellular PDI can activate integrins and tissue factor, which are involved respectively in pro-inflammatory responses and disseminated intravascular coagulation, both of which manifest in many viral haemorrhagic fevers. The discovery of enhanced PDI secretion from NSDV-infected cells may be an important finding for understanding the mechanisms underlying the pathogenicity of haemorrhagic nairoviruses.
[Mh] Termos MeSH primário: Membrana Celular/metabolismo
Retículo Endoplasmático/metabolismo
Espaço Extracelular/metabolismo
Doença dos Ovinos de Nairobi/metabolismo
Vírus da Doença do Carneiro de Nairobi/fisiologia
Isomerases de Dissulfetos de Proteínas/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Cercopithecus aethiops
Glicoproteínas/metabolismo
Cabras
Complexo de Golgi/metabolismo
Ligação Proteica
Transporte Proteico
Células Vero
Proteínas Virais/metabolismo
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Glycoproteins); 0 (Viral Proteins); EC 5.3.4.1 (Protein Disulfide-Isomerases)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140410
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0094656


  5 / 11 MEDLINE  
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[PMID]:23083136
[Au] Autor:Bin Tarif A; Lasecka L; Holzer B; Baron MD
[Ad] Endereço:The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey, GU24 0NF, United Kingdom. michael.baron@pirbright.ac.uk.
[Ti] Título:Ganjam virus/Nairobi sheep disease virus induces a pro-inflammatory response in infected sheep.
[So] Source:Vet Res;43:71, 2012 Oct 19.
[Is] ISSN:1297-9716
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Partly due to climate change, and partly due to changes of human habitat occupation, the impact of tick-borne viruses is increasing. Nairobi sheep disease virus (NSDV) and Ganjam virus (GV) are two names for the same virus, which causes disease in sheep and goats and is currently known to be circulating in India and East Africa. The virus is transmitted by ixodid ticks and causes a severe hemorrhagic disease. We have developed a real-time PCR assay for the virus genome and validated it in a pilot study of the pathogenicity induced by two different isolates of NSDV/GV. One isolate was highly adapted to tissue culture, grew in most cell lines tested, and was essentially apathogenic in sheep. The second isolate appeared to be poorly adapted to cell culture and retained pathogenicity in sheep. The real-time PCR assay for virus easily detected 4 copies or less of the viral genome, and allowed a quantitative measure of the virus in whole blood. Measurement of the changes in cytokine mRNAs showed similar changes to those observed in humans infected by the closely related virus Crimean Congo hemorrhagic fever virus.
[Mh] Termos MeSH primário: Citocinas/genética
Doenças das Cabras/genética
Doença dos Ovinos de Nairobi/genética
Vírus da Doença do Carneiro de Nairobi/patogenicidade
Reação em Cadeia da Polimerase em Tempo Real/métodos
[Mh] Termos MeSH secundário: Animais
Citocinas/metabolismo
Doenças das Cabras/imunologia
Doenças das Cabras/virologia
Cabras
Doença dos Ovinos de Nairobi/imunologia
Doença dos Ovinos de Nairobi/virologia
Vírus da Doença do Carneiro de Nairobi/classificação
Vírus da Doença do Carneiro de Nairobi/genética
Vírus da Doença do Carneiro de Nairobi/isolamento & purificação
RNA Viral/análise
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Ovinos
Virulência
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Cytokines); 0 (RNA, Viral)
[Em] Mês de entrada:1406
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121023
[St] Status:MEDLINE
[do] DOI:10.1186/1297-9716-43-71


  6 / 11 MEDLINE  
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[PMID]:22163042
[Au] Autor:Holzer B; Bakshi S; Bridgen A; Baron MD
[Ad] Endereço:Institute for Animal Health, Pirbright, Surrey, United Kingdom.
[Ti] Título:Inhibition of interferon induction and action by the nairovirus Nairobi sheep disease virus/Ganjam virus.
[So] Source:PLoS One;6(12):e28594, 2011.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Nairoviruses are an important group of tick-borne viruses that includes pathogens of man (Crimean Congo hemorrhagic fever virus) and livestock animals (Dugbe virus, Nairobi sheep disease virus (NSDV)). NSDV is found in large parts of East Africa and the Indian subcontinent (where it is known as Ganjam virus). We have investigated the ability of NSDV to antagonise the induction and actions of interferon. Both pathogenic and apathogenic isolates could actively inhibit the induction of type 1 interferon, and also blocked the signalling pathways of both type 1 and type 2 interferons. Using transient expression of viral proteins or sections of viral proteins, these activities all mapped to the ovarian tumour-like protease domain (OTU) found in the viral RNA polymerase. Virus infection, or expression of this OTU domain in transfected cells, led to a great reduction in the incorporation of ubiquitin or ISG15 protein into host cell proteins. Point mutations in the OTU that inhibited the protease activity also prevented it from antagonising interferon induction and action. Interestingly, a mutation at a peripheral site, which had little apparent effect on the ability of the OTU to inhibit ubiquitination and ISG15ylation, removed the ability of the OTU to block the induction of type 1 and the action of type 2 interferons, but had a lesser effect on the ability to block type 1 interferon action, suggesting that targets other than ubiquitin and ISG15 may be involved in the actions of the viral OTU.
[Mh] Termos MeSH primário: Interferons/metabolismo
Vírus da Doença do Carneiro de Nairobi/genética
[Mh] Termos MeSH secundário: Animais
Cercopithecus aethiops
RNA Polimerases Dirigidas por DNA/química
Genes Reporter
Cabras
Células HEK293
Seres Humanos
Imunidade Inata
Interferon beta/metabolismo
Interferon gama/metabolismo
Vírus da Doença do Carneiro de Nairobi/metabolismo
Fosforilação
Plasmídeos/metabolismo
Regiões Promotoras Genéticas
Estrutura Terciária de Proteína
Ovinos
Transcrição Genética
Ubiquitina/química
Células Vero
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ubiquitin); 77238-31-4 (Interferon-beta); 82115-62-6 (Interferon-gamma); 9008-11-1 (Interferons); EC 2.7.7.6 (DNA-Directed RNA Polymerases)
[Em] Mês de entrada:1207
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:111214
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0028594


  7 / 11 MEDLINE  
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[PMID]:21511058
[Au] Autor:Yadav PD; Vincent MJ; Khristova M; Kale C; Nichol ST; Mishra AC; Mourya DT
[Ad] Endereço:Microbial Containement Complex, National Institute of Virology, Sus Road, Pashan, Pune 410021, India.
[Ti] Título:Genomic analysis reveals Nairobi sheep disease virus to be highly diverse and present in both Africa, and in India in the form of the Ganjam virus variant.
[So] Source:Infect Genet Evol;11(5):1111-20, 2011 Jul.
[Is] ISSN:1567-7257
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Nairobi sheep disease (NSD) virus, the prototype tick-borne virus of the genus Nairovirus, family Bunyaviridae is associated with acute hemorrhagic gastroenteritis in sheep and goats in East and Central Africa. The closely related Ganjam virus found in India is associated with febrile illness in humans and disease in livestock. The complete S, M and L segment sequences of Ganjam and NSD virus and partial sequence analysis of Ganjam viral RNA genome S, M and L segments encoding regions (396 bp, 701 bp and 425 bp) of the viral nucleocapsid (N), glycoprotein precursor (GPC) and L polymerase (L) proteins, respectively, was carried out for multiple Ganjam virus isolates obtained from 1954 to 2002 and from various regions of India. M segments of NSD and Ganjam virus encode a large ORF for the glycoprotein precursor (GPC), (1627 and 1624 amino acids in length, respectively) and their L segments encode a very large L polymerase (3991 amino acids). The complete S, M and L segments of NSD and Ganjam viruses were more closely related to one another than to other characterized nairoviruses, and no evidence of reassortment was found. However, the NSD and Ganjam virus complete M segment differed by 22.90% and 14.70%, for nucleotide and amino acid respectively, and the complete L segment nucleotide and protein differing by 9.90% and 2.70%, respectively among themselves. Ganjam and NSD virus, complete S segment differed by 9.40-10.40% and 3.2-4.10 for nucleotide and proteins while among Ganjam viruses 0.0-6.20% and 0.0-1.4%, variation was found for nucleotide and amino acids. Ganjam virus isolates differed by up to 17% and 11% at the nucleotide level for the partial S and L gene fragments, respectively, with less variation observed at the deduced amino acid level (10.5 and 2%, S and L, respectively). However, the virus partial M gene fragment (which encodes the hypervariable mucin-like domain) of these viruses differed by as much as 56% at the nucleotide level. Phylogenetic analysis of partial sequence differences suggests considerable mixing and movement of Ganjam virus strains within India, with no clear relationship between genetic lineages and virus geographic origin or year of isolation. Surprisingly, NSD virus does not represent a distinct lineage, but appears as a variant with other Ganjam virus among NSD virus group.
[Mh] Termos MeSH primário: Vírus da Doença do Carneiro de Nairobi/genética
[Mh] Termos MeSH secundário: África/epidemiologia
Demografia
Variação Genética
Genoma Viral
Índia/epidemiologia
Filogenia
RNA Viral/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1109
[Cu] Atualização por classe:110530
[Lr] Data última revisão:
110530
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110423
[St] Status:MEDLINE
[do] DOI:10.1016/j.meegid.2011.04.001


  8 / 11 MEDLINE  
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[PMID]:20090098
[Au] Autor:Sudeep AB; Jadi RS; Mishra AC
[Ad] Endereço:Microbial Containment Complex, National Institute of Virology (ICMR), Pune, India. sudeepab@icmr.org.in
[Ti] Título:Ganjam virus.
[So] Source:Indian J Med Res;130(5):514-9, 2009 Nov.
[Is] ISSN:0971-5916
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:Ganjam virus (GANV), a member of genus Nairovirus of family Bunyavirdae is of considerable veterinary importance in India. Though, predominantly tick borne, GANV was also isolated from mosquitoes, man and sheep. Neutralizing and complement fixing antibodies to GANV have been detected in animal and human sera collected from different parts of the country. Thirty three strains of GANV have been isolated from India, mainly from Haemaphysalis ticks. The virus replicated in certain vertebrate and mosquito cell lines and found pathogenic to laboratory animals. One natural infection and five laboratory-acquired infections in men were also reported. GANV is antigenically related to Nairobi sheep disease virus (NSDV) of Africa, which is highly pathogenic for sheep and goats causing 70-90 per cent mortality among the susceptible population. Recent molecular studies have demonstrated that GANV is an Asian variant of NSDV and both these viruses are related to the dreaded Crimean Congo haemorrhagic fever (CCHF) group viruses. The versatility of the virus to replicate in different arthropod species, its ability to infect sheep, goat and man makes it an important zoonotic agent.
[Mh] Termos MeSH primário: Vírus da Doença do Carneiro de Nairobi/patogenicidade
[Mh] Termos MeSH secundário: Animais
Infecções por Bunyaviridae/transmissão
Infecções por Bunyaviridae/virologia
Feminino
Cabras
Seres Humanos
Índia
Masculino
Camundongos
Doença dos Ovinos de Nairobi/virologia
Vírus da Doença do Carneiro de Nairobi/genética
Vírus da Doença do Carneiro de Nairobi/isolamento & purificação
Vírus da Doença do Carneiro de Nairobi/fisiologia
Ovinos
Carrapatos/virologia
Replicação Viral
Zoonoses/transmissão
Zoonoses/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1004
[Cu] Atualização por classe:130418
[Lr] Data última revisão:
130418
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100122
[St] Status:MEDLINE


  9 / 11 MEDLINE  
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[PMID]:19193256
[Au] Autor:Crabtree MB; Sang R; Miller BR
[Ad] Endereço:Centers for Disease Control and Prevention, Fort Collins, Colorado, USA. mcrabtree@cdc.gov
[Ti] Título:Kupe virus, a new virus in the family bunyaviridae, genus nairovirus, kenya.
[So] Source:Emerg Infect Dis;15(2):147-54, 2009 Feb.
[Is] ISSN:1080-6059
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We have previously described isolation and preliminary identification of a virus related to Dugbe virus (DUGV), family Bunyaviridae, genus Nairovirus. Six isolates of the virus were obtained from pools of Amblyomma gemma and Rhipicephalus pulchellus ticks collected from hides of cattle in Nairobi, Kenya, in October 1999. We report results of further characterization of this virus, including growth kinetics in cell culture and full-length genome sequencing and genetic characterization, which show it to be distinct from DUGV. We suggest that this is a new virus in the family Bunyaviridae, genus Nairovirus, and we propose that it be designated Kupe virus.
[Mh] Termos MeSH primário: Ixodidae/virologia
Vírus da Doença do Carneiro de Nairobi/classificação
Vírus da Doença do Carneiro de Nairobi/genética
Rhipicephalus/virologia
[Mh] Termos MeSH secundário: Aedes/virologia
Animais
Bovinos
Doenças dos Bovinos/parasitologia
Linhagem Celular
Cercopithecus aethiops
Seres Humanos
Quênia
Vírus da Doença do Carneiro de Nairobi/crescimento & desenvolvimento
Vírus da Doença do Carneiro de Nairobi/isolamento & purificação
Filogenia
Análise de Sequência de DNA
Especificidade da Espécie
Infestações por Carrapato/parasitologia
Infestações por Carrapato/veterinária
Células Vero
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:0906
[Cu] Atualização por classe:141210
[Lr] Data última revisão:
141210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090206
[St] Status:MEDLINE


  10 / 11 MEDLINE  
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[PMID]:15799531
[Au] Autor:Joshi MV; Geevarghese G; Joshi GD; Ghodke YS; Mourya DT; Mishra AC
[Ad] Endereço:Division of Epidemiology National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411 001, Maharashtra, India. mvjoshi46@rediffmail.com
[Ti] Título:Isolation of Ganjam virus from ticks collected off domestic animals around Pune, Maharashtra, India.
[So] Source:J Med Entomol;42(2):204-6, 2005 Mar.
[Is] ISSN:0022-2585
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Studies on viruses of zoonotic importance in certain villages around Pune were undertaken between December 2000 and January 2002. A total of 1,138 adult ticks belonging to six different species were collected off domestic animals and processed for virus isolation. Six virus isolates were obtained. All six isolates were identified as Ganjam virus by Quick Complement Fixation test and reverse transcriptase-polymerase chain reaction using RNA nucleocapsid gene amplification. Five isolates were from the pools of adult Hemaphysalis intermedia ticks, and one isolate was from a pool of adult Rhipecephalus hemaphysaloides. This is the first report of isolation of Ganjam virus from Maharashtra state of India.
[Mh] Termos MeSH primário: Animais Domésticos
Vírus da Doença do Carneiro de Nairobi/isolamento & purificação
Carrapatos/virologia
[Mh] Termos MeSH secundário: Animais
Bovinos
Cabras
Índia
Rhipicephalus/virologia
População Rural
Ovinos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:0504
[Cu] Atualização por classe:050331
[Lr] Data última revisão:
050331
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:050401
[St] Status:MEDLINE



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde