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[PMID]:29091706
[Au] Autor:Pauvolid-Corrêa A; Campos Z; Soares R; Nogueira RMR; Komar N
[Ad] Endereço:Arbovirus Diseases Branch, Centers for Disease Control and Prevention (CDC), Fort Collins, CO, United States of America.
[Ti] Título:Neutralizing antibodies for orthobunyaviruses in Pantanal, Brazil.
[So] Source:PLoS Negl Trop Dis;11(11):e0006014, 2017 Nov.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Pantanal is a hotspot for arbovirus studies in South America. Various medically important flaviviruses and alphaviruses have been reported in domestic and wild animals in the region. To expand the knowledge of local arbovirus circulation, a serosurvey for 14 Brazilian orthobunyaviruses was conducted with equines, sheep and free-ranging caimans. Sera were tested for specific viral antibodies using plaque-reduction neutralization test (PRNT). Monotypic reactions were detected for Maguari, Xingu, Apeu, Guaroa, Murutucu, Oriboca, Oropouche and Nepuyo viruses. Despite the low titers for most of the orthobunyaviruses tested, the detection of monotypic reactions for eight orthobunyaviruses suggests the Pantanal as a region of great orthobunyavirus diversity. The present data, in conjunction with previous studies that detected a high diversity of other arboviruses, ratify the Pantanal as an important natural reservoir for sylvatic and medically important arboviruses in Brazil.
[Mh] Termos MeSH primário: Jacarés e Crocodilos
Anticorpos Neutralizantes/sangue
Anticorpos Antivirais/sangue
Infecções por Bunyaviridae/veterinária
Doenças dos Cavalos/epidemiologia
Orthobunyavirus/imunologia
Doenças dos Ovinos/epidemiologia
[Mh] Termos MeSH secundário: Jacarés e Crocodilos/virologia
Animais
Animais Domésticos/virologia
Animais Selvagens/virologia
Brasil/epidemiologia
Infecções por Bunyaviridae/epidemiologia
Infecções por Bunyaviridae/imunologia
Infecções por Bunyaviridae/virologia
Doenças dos Cavalos/imunologia
Doenças dos Cavalos/virologia
Cavalos/virologia
Orthobunyavirus/isolamento & purificação
Estudos Soroepidemiológicos
Ovinos/virologia
Doenças dos Ovinos/imunologia
Doenças dos Ovinos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Neutralizing); 0 (Antibodies, Viral)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171102
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0006014


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[PMID]:28937979
[Au] Autor:Yun SM; Park SJ; Park SW; Choi W; Jeong HW; Choi YK; Lee WJ
[Ad] Endereço:Division of Arboviruses, National Research Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju-si, Republic of Korea.
[Ti] Título:Molecular genomic characterization of tick- and human-derived severe fever with thrombocytopenia syndrome virus isolates from South Korea.
[So] Source:PLoS Negl Trop Dis;11(9):e0005893, 2017 Sep.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne viral disease caused by the SFTS virus (SFTSV) from Bunyaviridae that is endemic in East Asia. However, the genetic and evolutionary characteristics shared between tick- and human-derived Korean SFTSV strains are still limited. METHODOLOGY/PRINCIPAL FINDINGS: In this study we identify, for the first time, the genome sequence of a tick (Haemaphysalis longicornis)-derived Korean SFTSV strain (designated as KAGWT) and compare this virus with recent human SFTSV isolates to identify the genetic variations and relationships among SFTSV strains. The genome of the KAGWT strain is consistent with the described genome of other members of the genus Phlebovirus with 6,368 nucleotides (nt), 3,378 nt, and 1,746 nt in the Large (L), Medium (M) and Small (S) segments, respectively. Compared with other completely sequenced human-derived Korean SFTSV strains, the KAGWT strain had highest sequence identities at the nucleotide and deduced amino acid level in each segment with the KAGWH3 strain which was isolated from SFTS patient within the same region, although there is one unique amino acid substitution in the Gn protein (A66S). Phylogenetic analyses of complete genome sequences revealed that at least four different genotypes of SFTSV are co-circulating in South Korea, and that the tick- and human-derived Korean SFTSV strains (genotype B) are closely related to one another. Although we could not detect reassortant, which are commonly observed in segmented viruses, further large-scale surveillance and detailed genomic analysis studies are needed to better understand the molecular epidemiology, genetic diversity, and evolution of SFTSV. CONCLUSIONS/SIGNIFICANCE: Full-length sequence analysis revealed a clear association between the genetic origins of tick- and human-derived SFTSV strains. While the most prevalent Korean SFTSV is genotype B, at least four different genotypes of SFTSV strains are co-circulating in South Korea. These findings provide information regarding the molecular epidemiology, genetic diversity, and evolution of SFTSV in East Asia.
[Mh] Termos MeSH primário: Genoma Viral
Phlebovirus/genética
Phlebovirus/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Infecções por Bunyaviridae/complicações
Infecções por Bunyaviridae/epidemiologia
Infecções por Bunyaviridae/virologia
Variação Genética
Genótipo
Seres Humanos
Ixodidae/virologia
Orthobunyavirus/genética
Phlebovirus/patogenicidade
Filogenia
República da Coreia/epidemiologia
Análise de Sequência de DNA
Síndrome
Trombocitopenia/complicações
Trombocitopenia/epidemiologia
Trombocitopenia/virologia
Doenças Transmitidas por Carrapatos/epidemiologia
Doenças Transmitidas por Carrapatos/virologia
Carrapatos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170923
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005893


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[PMID]:28821698
[Ti] Título:Schmallenberg virus infection in Scottish cattle.
[So] Source:Vet Rec;181(8):189-192, 2017 Aug 19.
[Is] ISSN:2042-7670
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Schmallenberg virus infection on cattle farms in southern ScotlandOutbreaks of infectious bovine rhinotracheitis Bovismorbificans infection in cattle and sheepGastric ulceration in pigsTrichomonosis in a sparrowhawk and a hen harrierThese are among matters discussed in the disease surveillance report for April 2017 from SAC Consulting: Veterinary Services (SAC C VS).
[Mh] Termos MeSH primário: Doenças dos Animais/epidemiologia
Surtos de Doenças/veterinária
Vigilância de Evento Sentinela/veterinária
[Mh] Termos MeSH secundário: Animais
Doenças das Aves/epidemiologia
Aves
Infecções por Bunyaviridae/epidemiologia
Infecções por Bunyaviridae/veterinária
Bovinos
Doenças dos Bovinos/epidemiologia
Doenças dos Bovinos/virologia
Cervos
Feminino
Orthobunyavirus/isolamento & purificação
Gravidez
Escócia/epidemiologia
Ovinos
Doenças dos Ovinos/epidemiologia
Suínos
Doenças dos Suínos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170820
[St] Status:MEDLINE
[do] DOI:10.1136/vr.j3771


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[PMID]:28813443
[Au] Autor:García-Bocanegra I; Cano-Terriza D; Vidal G; Rosell R; Paniagua J; Jiménez-Ruiz S; Expósito C; Rivero-Juarez A; Arenas A; Pujols J
[Ad] Endereço:Departamento de Sanidad Animal, Universidad de Córdoba-Agrifood Excellence International Campus (ceiA3), Córdoba, Spain.
[Ti] Título:Monitoring of Schmallenberg virus in Spanish wild artiodactyls, 2006-2015.
[So] Source:PLoS One;12(8):e0182212, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Schmallenberg disease is an emerging disease that affects domestic and wild ruminants in Europe. An epidemiological survey was carried out to assess exposure to Schmallenberg virus (SBV) in wild artiodactyls in Spain between 2006 and 2015. A total of 1751 sera from wild artiodactyls, including 1066 red deer, 304 fallow deer, 192 mouflon, 109 wild boar, 49 roe deer and 31 Spanish ibex were tested for antibodies against SBV by ELISA and confirmed by virus neutralization test. SBV was not detected between the 2006/2007 and the 2010/2011 hunting seasons. Overall seroprevalence (including samples collected between the 2011/2012 and 2014/2015 hunting seasons) was 14.6% (160/1099; 95%CI: 12.7-16.6). Mean SBV seroprevalence was 13.3±2.6% in red deer, 23.9±4.2% in fallow deer, 16.4±6.1% in mouflon and 2.8±3.1% in wild boar. No antibodies against SBV were found in roe deer or Spanish ibex. The presence of SBV RNA was confirmed in three of 255 (1.2%) spleen samples from wild ruminants analysed by rRT-PCR. In a multivariate mixed-effects logistic regression model, the main risk factors associated with SBV seroprevalence were: species (fallow deer, red deer and mouflon), age (adults) and interactions between hunting areas of more than 1000 hectares and hunting season (2012/2013, 2013/2014 and 2014/2015). The hypothesis of endemic circulation of SBV in the last few years is supported by the detection of SBV RNA in animals sampled in 2011 and 2015, as well as antibodies detected at low level in juveniles in 2012, 2013 and 2014. The results indicate that SBV circulated in wild ruminant populations in Spain during the same period when the virus was first reported in northern Europe, and at least five months before the first case was officially reported in livestock in Spain.
[Mh] Termos MeSH primário: Doenças dos Animais/epidemiologia
Doenças dos Animais/virologia
Animais Selvagens
Infecções por Bunyaviridae/veterinária
Orthobunyavirus
Vigilância em Saúde Pública
Ruminantes
[Mh] Termos MeSH secundário: Doenças dos Animais/história
Animais
Geografia Médica
História do Século XXI
Orthobunyavirus/genética
Orthobunyavirus/imunologia
Prevalência
Fatores de Risco
Estudos Soroepidemiológicos
Espanha/epidemiologia
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170817
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182212


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[PMID]:28699878
[Au] Autor:Poskin A; Martinelle L; Van der Stede Y; Saegerman C; Cay B; De Regge N
[Ad] Endereço:1​CODA-CERVA, Operational Directorate Viral Diseases, Groeselenberg 99, 1180 Brussels, Belgium.
[Ti] Título:Genetically stable infectious Schmallenberg virus persists in foetal envelopes of pregnant ewes.
[So] Source:J Gen Virol;98(7):1630-1635, 2017 Jul.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Schmallenberg virus (SBV) is a recently emerged vector-borne virus, inducing congenital defects in bovines, ovines and caprines. Here we have shown that infectious SBV is capable of persisting until the moment of birth in the foetal envelopes of ewes infected with SBV-infectious serum at day 45 (1/5 positive) and 60 (4/6 positive) of gestation. This persistence of at least 100 days is a new aspect of the SBV pathogenesis that could help to explain how SBV overwinters the cold season in temperate climate zones. Furthermore, sequencing of the M segment shows that the persisting virus in the foetal envelopes is genetically stable since only a few mutations compared to the inoculum were found. This supports the hypothesis that persisting virus could start the infection of new hosts. Finally, neutralization tests showed that infectious SBV present in the foetal envelopes at birth can be neutralized by the humoral immunity present in the infected ewes.
[Mh] Termos MeSH primário: Infecções por Bunyaviridae/virologia
Córion/virologia
Orthobunyavirus/genética
Placenta/virologia
RNA Viral/genética
Doenças dos Ovinos/virologia
Ovinos/virologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/imunologia
Sequência de Bases
Infecções por Bunyaviridae/imunologia
Feminino
Orthobunyavirus/imunologia
Orthobunyavirus/isolamento & purificação
Gravidez
Análise de Sequência de RNA
Doenças dos Ovinos/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (RNA, Viral)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170804
[Lr] Data última revisão:
170804
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000841


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[PMID]:28640745
[Au] Autor:Roman-Sosa G; Karger A; Kraatz F; Aebischer A; Wernike K; Maksimov P; Lillig CH; Reimann I; Brocchi E; Keller M; Beer M
[Ad] Endereço:†â€‹Present address: Unité de Virologie Structurale, Institut Pasteur, France. 1​Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
[Ti] Título:The amino terminal subdomain of glycoprotein Gc of Schmallenberg virus: disulfide bonding and structural determinants of neutralization.
[So] Source:J Gen Virol;98(6):1259-1273, 2017 Jun.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Orthobunyaviruses are enveloped viruses that can cause human and animal diseases. A novel and major member is the Schmallenberg virus (SBV), the etiological agent of an emerging disease of ruminants that has been spreading all over Europe since 2011. The glycoproteins Gn and Gc of orthobunyaviruses mediate the viral entry, and specifically Gc is a major target for the humoral immune response. For example, the N terminal subdomain of the SBV glycoprotein Gc is targeted by neutralizing monoclonal antibodies that recognize conformational epitopes. Here, we determined the structural features of the N terminus of Gc, and analysed its interaction with monoclonal antibodies. We were able to demonstrate that one of two N-glycosylation sites is essential for secretion and interaction with a subset of Gc-specific monoclonal antibodies. Furthermore, four disulfide bonds (S-S) were identified and the deletion of the third S-S blocked reactivity with another subset of mAbs with virus-neutralizing and non-neutralizing activity. The mutagenesis of the N-glycosylation sites and the disulfide bonds strongly indicated the independent folding of two subdomains within the SBV Gc N terminus. Further, the epitopes recognized by a panel of mAbs could be grouped into two clusters, as revealed by fine mapping using chimeric proteins. Combining the disulfide bonding and epitope mapping allowed us to generate a structural model of the SBV Gc N-terminus. This novel information about the role and structure of the amino terminal region of SBV Gc is of general relevance for the design of antivirals and vaccines against this virus.
[Mh] Termos MeSH primário: Glicoproteínas/química
Glicoproteínas/imunologia
Orthobunyavirus/química
Orthobunyavirus/imunologia
Proteínas Virais/química
Proteínas Virais/imunologia
Internalização do Vírus
[Mh] Termos MeSH secundário: Animais
Anticorpos Monoclonais/imunologia
Anticorpos Monoclonais/metabolismo
Anticorpos Neutralizantes/imunologia
Anticorpos Neutralizantes/metabolismo
Linhagem Celular
Dicroísmo Circular
Análise Mutacional de DNA
Dissulfetos
Glicoproteínas/genética
Seres Humanos
Modelos Biológicos
Modelos Moleculares
Testes de Neutralização
Orthobunyavirus/genética
Orthobunyavirus/fisiologia
Ligação Proteica
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Antibodies, Neutralizing); 0 (Disulfides); 0 (Glycoproteins); 0 (Viral Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170717
[Lr] Data última revisão:
170717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000810


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[PMID]:28628828
[Au] Autor:Varela M; Piras IM; Mullan C; Shi X; Tilston-Lunel NL; Pinto RM; Taggart A; Welch SR; Neil SJD; Kreher F; Elliott RM; Palmarini M
[Ad] Endereço:MRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, Scotland, United Kingdom. Electronic address: mariana.varela@glasgow.ac.uk.
[Ti] Título:Sensitivity to BST-2 restriction correlates with Orthobunyavirus host range.
[So] Source:Virology;509:121-130, 2017 Sep.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Orthobunyaviruses include several recently emerging viruses of significant medical and veterinary importance. There is currently very limited understanding on what determines the host species range of these pathogens. In this study we discovered that BST-2/tetherin restricts orthobunyavirus replication in a host-specific manner. We show that viruses with human tropism (Oropouche virus and La Crosse virus) are restricted by sheep BST-2 but not by the human orthologue, while viruses with ruminant tropism (Schmallenberg virus and others) are restricted by human BST-2 but not by the sheep orthologue. We also show that BST-2 blocks orthobunyaviruses replication by reducing the amount of envelope glycoprotein into viral particles egressing from infected cells. This is the first study identifying a restriction factor that correlates with species susceptibility to orthobunyavirus infection. This work provides insight to help us dissect the adaptive changes that bunyaviruses require to cross the species barrier and emerge into new species.
[Mh] Termos MeSH primário: Antígenos CD/metabolismo
Antígenos de Diferenciação de Linfócitos B/metabolismo
Especificidade de Hospedeiro
Interações Hospedeiro-Patógeno
Orthobunyavirus/imunologia
Orthobunyavirus/fisiologia
Proteínas do Envelope Viral/antagonistas & inibidores
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Proteínas Ligadas por GPI/metabolismo
Seres Humanos
Ovinos
Liberação de Vírus
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (Antigens, Differentiation, B-Lymphocyte); 0 (BST2 protein, human); 0 (GPI-Linked Proteins); 0 (Viral Envelope Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170902
[Lr] Data última revisão:
170902
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE


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[PMID]:28600415
[Ti] Título:Schmallenberg virus infection confirmed in Scotland.
[So] Source:Vet Rec;180(23):562-565, 2017 Jun 10.
[Is] ISSN:2042-7670
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Schmallenberg virus infection in malformed lambsFood chain issues after lead poisoning incidentSuspected ragwort poisoning in heifersEwe abortions due to serovar Urbana detected in aborted lambsThese are among matters discussed in the disease surveillance report for February 2017 from SAC Consulting: Veterinary Services (SAC C VS).
[Mh] Termos MeSH primário: Doenças dos Bovinos/epidemiologia
Vigilância de Evento Sentinela/veterinária
Doenças dos Ovinos/epidemiologia
[Mh] Termos MeSH secundário: Animais
Infecções por Bunyaviridae/epidemiologia
Infecções por Bunyaviridae/veterinária
Bovinos
Doenças dos Bovinos/virologia
Feminino
Masculino
Orthobunyavirus/isolamento & purificação
Gravidez
Escócia/epidemiologia
Ovinos
Doenças dos Ovinos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170615
[Lr] Data última revisão:
170615
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170611
[St] Status:MEDLINE
[do] DOI:10.1136/vr.j2602


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Vasconcelos, Pedro Fernando da Costa
Texto completo SciELO Brasil
[PMID]:28591313
[Au] Autor:Naveca FG; Nascimento VAD; Souza VC; Nunes BTD; Rodrigues DSG; Vasconcelos PFDC
[Ad] Endereço:Fundação Oswaldo Cruz-Fiocruz, Instituto Leônidas e Maria Deane, Manaus, AM, Brasil.
[Ti] Título:Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses.
[So] Source:Mem Inst Oswaldo Cruz;112(7):510-513, 2017 Jul.
[Is] ISSN:1678-8060
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:We describe a sensitive method for simultaneous detection of Oropouche and Oropouche-like viruses carrying the Oropouche S segment, as well as the Mayaro virus, using a multiplexed one-step reverse transcription real-time polymerase chain reaction (RT-qPCR). A chimeric plasmid containing both Mayaro and Oropouche targets was designed and evaluated for the in vitro production of transcribed RNA, which could be easily used as a non-infectious external control. To track false-negative results due to PCR inhibition or equipment malfunction, the MS2 bacteriophage was also included in the multiplex assay as an internal positive control. The specificity of the multiplex assay was evaluated by Primer-Blast analysis against the entire GenBank database, and further against a panel of 17 RNA arboviruses. The results indicated an accurate and highly sensitive assay with amplification efficiency greater than 98% for both targets, and a limit of detection between two and 20 copies per reaction. We believe that the assay described here will provide a tool for Mayaro and Oropouche virus detection, especially in areas where differential diagnosis of Dengue, Zika and Chikungunya viruses should be performed.
[Mh] Termos MeSH primário: Infecções por Alphavirus/diagnóstico
Alphavirus/genética
Infecções por Bunyaviridae/diagnóstico
Orthobunyavirus/genética
[Mh] Termos MeSH secundário: Alphavirus/classificação
Infecções por Alphavirus/virologia
Infecções por Bunyaviridae/virologia
Seres Humanos
Reação em Cadeia da Polimerase Multiplex
Orthobunyavirus/classificação
Reprodutibilidade dos Testes
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170824
[Lr] Data última revisão:
170824
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE


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[PMID]:28539443
[Au] Autor:Murakami S; Takenaka-Uema A; Kobayashi T; Kato K; Shimojima M; Palmarini M; Horimoto T
[Ad] Endereço:Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan amurakam@mail.ecc.u-tokyo.ac.jp ahorimo@mail.ecc.u-tokyo.ac.jp.
[Ti] Título:Heparan Sulfate Proteoglycan Is an Important Attachment Factor for Cell Entry of Akabane and Schmallenberg Viruses.
[So] Source:J Virol;91(15), 2017 Aug 01.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:(AKAV) and Schmallenberg virus (SBV) are members of the genus , which are transmitted by arthropod vectors with a broad cellular tropism as well as Both AKAV and SBV cause arthrogryposis-hydranencephaly syndrome in ruminants. The main cellular receptor and attachment factor for entry of these orthobunyaviruses are unknown. Here, we found that AKAV and SBV infections were inhibited by the addition of heparin or enzymatic removal of cell surface heparan sulfates. To confirm this finding, we prepared heparan sulfate proteoglycan (HSPG)-knockout (KO) cells by using a clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system and measured the quantities of binding of these viruses to cell surfaces. We observed a substantial reduction in AKAV and SBV binding to cells, limiting the infections by these viruses. These data demonstrate that HSPGs are important cellular attachment factors for AKAV and SBV, at least , to promote virus replication in susceptible cells. AKAV and SBV are the etiological agents of arthrogryposis-hydranencephaly syndrome in ruminants, which causes considerable economic losses in the livestock industry. Here, we identified heparan sulfate proteoglycan as a major cellular attachment factor for the entry of AKAV and SBV. Moreover, we found that heparin is a strong inhibitor of AKAV and SBV infections. Revealing the molecular mechanisms of virus-host interactions is critical in order to understand virus biology and develop novel live attenuated vaccines.
[Mh] Termos MeSH primário: Proteoglicanas de Heparan Sulfato/metabolismo
Orthobunyavirus/fisiologia
Receptores Virais/metabolismo
Ligação Viral
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Cricetinae
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Heparan Sulfate Proteoglycans); 0 (Receptors, Virus)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE



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