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  1 / 161 MEDLINE  
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[PMID]:27605372
[Au] Autor:Bara JJ; Parker AT; Muturi EJ
[Ad] Endereço:Current address: Department of Biology, University of Louisville, Louisville KY 40209 (jay.bara@gmail.com) jay.bara@gmail.com.
[Ti] Título:Comparative Susceptibility of Ochlerotatus japonicus, Ochlerotatus triseriatus, Aedes albopictus, and Aedes aegypti (Diptera: Culicidae) to La Crosse Virus.
[So] Source:J Med Entomol;53(6):1415-1421, 2016 11.
[Is] ISSN:1938-2928
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Invasive mosquito species can increase the transmission risk of native mosquito-borne diseases by acting as novel vectors. In this study, we examined the susceptibility of three exotic invasive mosquito species Aedes aegypti (L.), Ae. albopictus (Skuse), and Ochlerotatus japonicus (Theobald) to La Crosse virus (LACV) relative to the native primary vector Ochlerotatus triseriatus (Say). Adult females of the four mosquito species were orally challenged with LACV; incubated for 3, 5, 7, 9, or 11 d; and their midgut infection rates, dissemination rates, and effective vector competence were determined. Overall, Oc. japonicus (2.92) had the highest effective vector competence values, followed by Ae. albopictus (1.55), Ae. aegypti (0.88), and Oc. triseriatus (0.64). In addition, we assessed the relationship between mosquito size and LACV susceptibility for field-collected Oc. triseriatus and Oc. japonicus We hypothesized that smaller adults would be more susceptible to LACV; however, our results did not support this hypothesis. Infected Oc. triseriatus tended to be larger than exposed but uninfected females, while infected and uninfected Oc. japonicus were similarly sized. These findings suggest that Oc. japonicus, Ae. albopictus, and Ae. aegypti have significant potential to transmit LACV and more research is needed to uncover their potential role in LACV epidemiology.
[Mh] Termos MeSH primário: Aedes/virologia
Insetos Vetores/virologia
Vírus La Crosse/fisiologia
Ochlerotatus/virologia
[Mh] Termos MeSH secundário: Animais
Feminino
Espécies Introduzidas
Estados Unidos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160909
[St] Status:MEDLINE


  2 / 161 MEDLINE  
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[PMID]:27357374
[Au] Autor:Muturi EJ; Bara JJ; Rooney AP; Hansen AK
[Ad] Endereço:Illinois Natural History Survey, University of Illinois at Urbana-Champaign, 1816 S. Oak St., Champaign, IL, 61820, USA.
[Ti] Título:Midgut fungal and bacterial microbiota of Aedes triseriatus and Aedes japonicus shift in response to La Crosse virus infection.
[So] Source:Mol Ecol;25(16):4075-90, 2016 Aug.
[Is] ISSN:1365-294X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Understanding how midgut microbial communities of field-collected mosquitoes interact with pathogens is critical for controlling vector infection and disease. We used 16S rRNA and internal transcribed spacer sequencing to characterize the midgut bacterial and fungal communities of adult females of Aedes triseriatus and Aedes japonicus collected as pupae in tree holes, plastic bins and waste tires and their response to La Crosse virus (LACV) infection. For both mosquito species and across all habitat and virus treatments, a total of 62 bacterial operational taxonomic units (OTUs) from six phyla and 21 fungal OTUs from two phyla were identified. The majority of bacterial (92%) and fungal (71%) OTUs were shared between the mosquito species; however, several OTUs were unique to each species. Bacterial and fungal communities of individuals that took either infectious or noninfectious bloodmeals were less diverse and more homogeneous compared to those of newly emerged adults. Interestingly, LACV-infected A. triseriatus and A. japonicus had higher bacterial richness and lower fungal richness compared to individuals that took a noninfectious bloodmeal, suggesting that viral infection was associated with an increase in bacterial OTUs and a decrease in fungal OTUs. For both mosquito species, several OTUs were identified that had both high fidelity and specificity to mosquito midguts that were infected with LACV. Overall, these findings demonstrate that bacterial and fungal communities that reside in mosquito midguts respond to host diet and viral infection and could play a role in modulating vector susceptibility to LACV.
[Mh] Termos MeSH primário: Aedes/microbiologia
Aedes/virologia
Vírus La Crosse
Microbiota
[Mh] Termos MeSH secundário: Animais
Bactérias/classificação
DNA Espaçador Ribossômico/genética
Feminino
Fungos/classificação
Interações Hospedeiro-Patógeno
Mosquitos Vetores/microbiologia
Mosquitos Vetores/virologia
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Ribosomal Spacer); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160701
[St] Status:MEDLINE
[do] DOI:10.1111/mec.13741


  3 / 161 MEDLINE  
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[PMID]:27105213
[Au] Autor:Urquhart C; Paulsen D; Moncayo A; Trout Fryxell RT
[Ad] Endereço:1 Department of Entomology and Plant Pathology, University of Tennessee, 2505 EJ Chapman Drive, Knoxville, TN 37996.
[Ti] Título:Evaluating Surveillance Methods for Arboviral Vectors of La Crosse Virus and West Nile Virus of Southern Appalachia.
[So] Source:J Am Mosq Control Assoc;32(1):24-33, 2016 Mar.
[Is] ISSN:8756-971X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To monitor mosquito-borne diseases, public health departments conduct mosquito and pathogen surveillance. Our objective was to evaluate mosquito monitoring methods for collecting La Crosse virus (LACV) and West Nile virus (WNV) vectors (Aedes and Culex mosquitoes, respectively) in southern Appalachia. Centers for Disease Control and Prevention (CDC) light traps baited with carbon dioxide (CO(2)), CDC light traps baited with CO(2) and BG lure, BG-Sentinel traps baited with CO(2), gravid traps baited with oak (Quercus)-water infusion, and resting traps were compared in eastern Tennessee in 2013. Traps operated at 8 different urban sites throughout Knox County were randomly assigned to and rotated among 6 plots within each site. Results were specific for each vector; the BG-Sentinel trap was the best method for Aedes triseriatus, the CDC trap baited with CO(2) and BG lure was the best method for Ae. albopictus, and the gravid trap was the best method for Ae. japonicus. Culex erraticus collections varied by week and trapping method, indicating no single method was best, but the questing traps collected more mosquitoes. There was no significant trapping difference for Cx. pipiens complex in this region using the methods tested. The results suggest using a combination of trapping methods when sampling for LACV and/or WNV mosquito vectors in southern Appalachia. Effective trapping methods are necessary to enable accurate surveillance, improve control methods, enhance understanding of dispersal, and use for early detection of vectors and pathogens.
[Mh] Termos MeSH primário: Aedes/fisiologia
Culex/fisiologia
Insetos Vetores/fisiologia
Vírus La Crosse/isolamento & purificação
Vírus do Nilo Ocidental/isolamento & purificação
[Mh] Termos MeSH secundário: Aedes/virologia
Distribuição Animal
Animais
Região dos Apalaches
Culex/virologia
Insetos Vetores/virologia
Densidade Demográfica
Tennessee
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1607
[Cu] Atualização por classe:160423
[Lr] Data última revisão:
160423
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160423
[St] Status:MEDLINE
[do] DOI:10.2987/8756-971X-32.1.24


  4 / 161 MEDLINE  
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[PMID]:27086496
[Au] Autor:Teleron AL; Rose BK; Williams DM; Kemper SE; McJunkin JE
[Ad] Endereço:Department of Internal Medicine, West Virginia University Charleston Division. Electronic address: amylynn.teleron@uvmhealth.org.
[Ti] Título:La Crosse Encephalitis: An Adult Case Series.
[So] Source:Am J Med;129(8):881-4, 2016 Aug.
[Is] ISSN:1555-7162
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: La Crosse viral encephalitis is well described in children, but to date, there are only 2 adult cases described in the literature. Despite the fact that pediatric infection can be life threatening and typically presents as a febrile meningoencephalitis often complicated by seizures and mental status changes, little is known about the presentation and course of adult infection. We report the largest case series of adult La Crosse encephalitis. METHODS: Inpatient data were reviewed between 2001 and 2012 to identify adults (≥18 years of age) with possible La Crosse encephalitis. Subsequent review of serologic testing was followed by a comprehensive chart review. RESULTS: Ten cases were identified, with ages ranging from 20 to 80 years. Fever, headache, and hyponatremia were seen in the majority, while mental status changes occurred in 5 patients and seizures in 2 patients. The mean length of stay was 8.4 days (± 8.4); 3 patients required intensive care unit admission, 2 of them were intubated, and 4 patients required discharge to a rehabilitation facility. CONCLUSIONS: La Crosse Virus produces a clinically significant encephalitis in adults, and a high level of suspicion should be maintained, particularly in endemic areas. There were no deaths, but La Crosse encephalitis in adults remained a morbid illness often associated with mental status changes, prolonged length of stay or intensive care unit admission, and frequent need for postdischarge rehabilitation.
[Mh] Termos MeSH primário: Encefalite da Califórnia/epidemiologia
Vírus La Crosse
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Animais
Feminino
Seres Humanos
Masculino
Meia-Idade
Estudos Retrospectivos
West Virginia/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170508
[Lr] Data última revisão:
170508
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160419
[St] Status:MEDLINE


  5 / 161 MEDLINE  
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[PMID]:26906411
[Au] Autor:D'Angelo W; Acharya D; Wang R; Wang J; Gurung C; Chen B; Bai F; Guo YL
[Ad] Endereço:1 Department of Biological Sciences, the University of Southern Mississippi , Hattiesburg, Mississippi.
[Ti] Título:Development of Antiviral Innate Immunity During In Vitro Differentiation of Mouse Embryonic Stem Cells.
[So] Source:Stem Cells Dev;25(8):648-59, 2016 Apr 15.
[Is] ISSN:1557-8534
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The innate immunity of embryonic stem cells (ESCs) has recently emerged as an important issue in ESC biology and in ESC-based regenerative medicine. We have recently reported that mouse ESCs (mESCs) do not have a functional type I interferon (IFN)-based antiviral innate immunity. They are deficient in expressing IFN in response to viral infection and have limited ability to respond to IFN. Using fibroblasts (FBs) as a cell model, the current study investigated the development of antiviral mechanisms during in vitro differentiation of mESCs. We demonstrate that mESC-differentiated FBs (mESC-FBs) share extensive similarities with naturally differentiated FBs in morphology, marker expression, and growth pattern, but their development of antiviral mechanisms lags behind. Nonetheless, the antiviral mechanisms are inducible during mESC differentiation as demonstrated by the transition of nuclear factor kappa B (NFκB), a key transcription factor for IFN expression, from its inactive state in mESCs to its active state in mESC-FBs and by increased responses of mESC-FBs to viral stimuli and IFN during their continued in vitro propagation. Together with our previously published study, the current data provide important insights into molecular basis for the deficiency of IFN expression in mESCs and the development of antiviral innate immunity during mESC differentiation.
[Mh] Termos MeSH primário: Diferenciação Celular
Imunidade Inata
Células-Tronco Embrionárias Murinas/imunologia
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Cercopithecus aethiops
Vírus Chikungunya/imunologia
Técnicas de Cocultura
Interferon Tipo I/metabolismo
Vírus La Crosse/imunologia
Camundongos
Células-Tronco Embrionárias Murinas/fisiologia
Células-Tronco Embrionárias Murinas/virologia
NF-kappa B/metabolismo
Medicina Regenerativa
Células Vero
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Interferon Type I); 0 (NF-kappa B)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170415
[Lr] Data última revisão:
170415
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160225
[St] Status:MEDLINE
[do] DOI:10.1089/scd.2015.0377


  6 / 161 MEDLINE  
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[PMID]:26477049
[Au] Autor:Westby KM; Muturi EJ; Juliano SA
[Ad] Endereço:School of Biological Sciences, Illinois State University, Normal, IL 61790-4120 (katiewestby206@gmail.com; sajulian@ilstu.edu), Current address: Washington University in St. Louis, Tyson Research Center, 6750 Tyson Valley Rd., Eureka, MO 63025, katiewestby206@gmail.com.
[Ti] Título:How do Nutritional Stress and La Crosse Virus Infection Interact? Tests for Effects on Willingness to Blood Feed and Fecundity in Aedes albopictus (Diptera: Culicidae).
[So] Source:J Med Entomol;53(1):166-71, 2016 Jan.
[Is] ISSN:0022-2585
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Evolutionary theory predicts that vector-borne pathogens should have low virulence for their vector because of selection against pathogens that harm the vector sufficiently to reduce transmission. Environmental factors such as nutritional stress can alter vector-pathogen associations by making the vectors more susceptible to pathogens (condition-dependent competence) and vulnerable to the harm caused by pathogen replication (condition-dependent virulence). We tested the hypotheses of condition-dependent competence and condition-dependent virulence by examining the interactive effects of short-term sugar deprivation and exposure to La Crosse virus (LACV) in female Aedes albopictus (Skuse). We predicted that infection status interacts with sugar deprivation to alter willingness to blood feed and fecundity in the second gonotrophic cycle (condition-dependent virulence). Sugar deprivation had no effect on body infection or disseminated infection rates. Infection status, sugar treatment, and their interaction had no effect on fecundity. Mosquitoes that had intermittent access to sugar were significantly more willing to take a second bloodmeal compared with those that had continuous access to sugar. Infection status and the interaction with sugar treatment had no effect on blood-feeding behavior. Thus, we found no evidence of short-term sugar deprivation leading to condition-dependent competence for, or condition-dependent virulence of, LACV in Ae. albopictus.
[Mh] Termos MeSH primário: Aedes/virologia
Privação de Alimentos/fisiologia
Interações Hospedeiro-Patógeno
Vírus La Crosse/fisiologia
[Mh] Termos MeSH secundário: Aedes/fisiologia
Animais
Encefalite da Califórnia/transmissão
Comportamento Alimentar
Feminino
Fertilidade
Insetos Vetores/fisiologia
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1604
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151019
[St] Status:MEDLINE
[do] DOI:10.1093/jme/tjv146


  7 / 161 MEDLINE  
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[PMID]:26523970
[Au] Autor:Miner JJ; Daniels BP; Shrestha B; Proenca-Modena JL; Lew ED; Lazear HM; Gorman MJ; Lemke G; Klein RS; Diamond MS
[Ad] Endereço:Department of Medicine, Washington University School of Medicine, Saint Louis, Missouri, USA.
[Ti] Título:The TAM receptor Mertk protects against neuroinvasive viral infection by maintaining blood-brain barrier integrity.
[So] Source:Nat Med;21(12):1464-72, 2015 Dec.
[Is] ISSN:1546-170X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The TAM receptors Tyro3, Axl and Mertk are receptor tyrosine kinases that dampen host innate immune responses following engagement with their ligands Gas6 and Protein S, which recognize phosphatidylserine on apoptotic cells. In a form of apoptotic mimicry, many enveloped viruses display phosphatidylserine on the outer leaflet of their membranes, enabling TAM receptor activation and downregulation of antiviral responses. Accordingly, we hypothesized that a deficiency of TAM receptors would enhance antiviral responses and protect against viral infection. Unexpectedly, mice lacking Mertk and/or Axl, but not Tyro3, exhibited greater vulnerability to infection with neuroinvasive West Nile and La Crosse encephalitis viruses. This phenotype was associated with increased blood-brain barrier permeability, which enhanced virus entry into and infection of the brain. Activation of Mertk synergized with interferon-ß to tighten cell junctions and prevent virus transit across brain microvascular endothelial cells. Because TAM receptors restrict pathogenesis of neuroinvasive viruses, these findings have implications for TAM antagonists that are currently in clinical development.
[Mh] Termos MeSH primário: Barreira Hematoencefálica/enzimologia
Barreira Hematoencefálica/virologia
Encefalite da Califórnia/enzimologia
Proteínas Proto-Oncogênicas/metabolismo
Receptores Proteína Tirosina Quinases/metabolismo
Febre do Nilo Ocidental/enzimologia
[Mh] Termos MeSH secundário: Imunidade Adaptativa
Animais
Astrócitos/metabolismo
Astrócitos/patologia
Barreira Hematoencefálica/patologia
Quimiocinas/sangue
Encefalite da Califórnia/patologia
Encefalite da Califórnia/virologia
Células Endoteliais/metabolismo
Células Endoteliais/patologia
Interferon beta/metabolismo
Vírus La Crosse/fisiologia
Camundongos Endogâmicos C57BL
Camundongos Knockout
Microvasos/patologia
Permeabilidade
Substâncias Protetoras
Proteínas Proto-Oncogênicas/antagonistas & inibidores
Proteínas Proto-Oncogênicas/deficiência
Tolerância a Radiação
Receptores Proteína Tirosina Quinases/antagonistas & inibidores
Receptores Proteína Tirosina Quinases/deficiência
Transdução de Sinais
Análise de Sobrevida
Carga Viral
Febre do Nilo Ocidental/patologia
Febre do Nilo Ocidental/virologia
Vírus do Nilo Ocidental/fisiologia
c-Mer Tirosina Quinase
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Chemokines); 0 (Protective Agents); 0 (Proto-Oncogene Proteins); 77238-31-4 (Interferon-beta); EC 2.7.10.1 (Mertk protein, mouse); EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases); EC 2.7.10.1 (Tyro3 protein, mouse); EC 2.7.10.1 (axl receptor tyrosine kinase); EC 2.7.10.1 (c-Mer Tyrosine Kinase)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151103
[St] Status:MEDLINE
[do] DOI:10.1038/nm.3974


  8 / 161 MEDLINE  
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[PMID]:26375904
[Au] Autor:Westby KM; Fritzen C; Paulsen D; Poindexter S; Moncayo AC
[Ad] Endereço:1 Vector-Borne Diseases Section, Tennessee Department of Health, 630 Hart Lane, Nashville, TN 37216.
[Ti] Título:La Crosse Encephalitis Virus Infection in Field-Collected Aedes albopictus, Aedes japonicus, and Aedes triseriatus in Tennessee.
[So] Source:J Am Mosq Control Assoc;31(3):233-41, 2015 Sep.
[Is] ISSN:8756-971X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:La Crosse virus (LACV) is a mosquito-borne virus and a major cause of pediatric encephalitis in the USA. La Crosse virus emerged in Tennessee and other states in the Appalachian region in 1997. We investigated LACV infection rates and seasonal abundances of the native mosquito vector, Aedes triseriatus, and 2 recently introduced mosquito species, Ae. albopictus and Ae. japonicus, in an emerging disease focus in Tennessee. Mosquitoes were collected using multiple trapping methods specific for Aedes mosquitoes at recent human case sites. Mosquito pools were tested via reverse transcriptase-polymerase chain reaction (RT-PCR) of the S segment to detect multiple Bunyamwera and California serogroup viruses, including LACV, as well as real-time RT-PCR of the M segment. A total of 54 mosquito pools were positive, including wild-caught adult females and laboratory-reared adults, demonstrating transovarial transmission in all 3 species. Maximum likelihood estimates (per 1,000 mosquitoes) were 2.72 for Ae. triseriatus, 3.01 for Ae. albopictus, and 0.63 for Ae. japonicus. We conclude that Ae. triseriatus and Ae. albopictus are important LACV vectors and that Ae. japonicus also may be involved in virus maintenance and transmission.
[Mh] Termos MeSH primário: Aedes/virologia
Insetos Vetores/virologia
Vírus La Crosse/isolamento & purificação
[Mh] Termos MeSH secundário: Aedes/fisiologia
Animais
Feminino
Seres Humanos
Insetos Vetores/fisiologia
Funções Verossimilhança
Dinâmica Populacional
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Estações do Ano
Especificidade da Espécie
Tennessee
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150917
[Lr] Data última revisão:
150917
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150917
[St] Status:MEDLINE
[do] DOI:10.2987/moco-31-03-233-241.1


  9 / 161 MEDLINE  
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[PMID]:26334821
[Au] Autor:Bara JJ; Muturi EJ
[Ad] Endereço:Illinois Natural History Survey, University of Illinois, Urbana, IL 61820. jjbara@illinois.edu.
[Ti] Título:Container Type Influences the Relative Abundance, Body Size, and Susceptibility of Ochlerotatus triseriatus (Diptera: Culicidae) to La Crosse Virus.
[So] Source:J Med Entomol;52(3):452-60, 2015 May.
[Is] ISSN:0022-2585
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ochlerotatus triseriatus (Say), the primary vector of La Crosse virus (LAC), develops in a variety of natural and artificial aquatic containers where it often co-occurs with larvae of other mosquito species. We conducted a field study at two woodlots (South Farms and Trelease Woods) in Urbana, IL, to examine how container type influences vector abundance, body size, and susceptibility to LAC. Mosquito pupae were collected from tree holes, plastic bins, and waste tires, and eclosing adults were identified to species morphologically. Oc. triseriatus and Ochlerotatus japonicus (Theobald) females were orally challenged with LAC and midgut infection rate, disseminated infection rate, and body titer were determined by reverse-transcriptase real-time PCR. Oc. triseriatus was the dominant species collected in tree holes while Oc. japonicus and Culex restuans (Theobald) were mostly dominant in artificial containers. Female Oc. triseriatus and Oc. japonicus collected from plastic bins were significantly larger than those collected from tree holes or waste tires. Oc. japonicus females from South Farms were also significantly larger than those from Trelease Woods. Oc. triseriatus females collected from plastic bins and waste tires were significantly more susceptible to LAC infection relative to females collected from tree holes. In addition, Oc. triseriatus females from waste tires had significantly higher LAC titer relative to Oc. triseriatus from tree holes. For each container type and study site, wing length was not correlated to infection or dissemination rates. These findings suggest that the container type in which Oc.triseriatus develop may contribute to the spatial and temporal dynamics of LAC transmission.
[Mh] Termos MeSH primário: Insetos Vetores/fisiologia
Insetos Vetores/virologia
Vírus La Crosse/fisiologia
Ochlerotatus/fisiologia
Ochlerotatus/virologia
[Mh] Termos MeSH secundário: Animais
Tamanho Corporal
Encefalite da Califórnia/transmissão
Encefalite da Califórnia/virologia
Feminino
Illinois
Insetos Vetores/crescimento & desenvolvimento
Larva/fisiologia
Larva/virologia
Masculino
Ochlerotatus/crescimento & desenvolvimento
Reação em Cadeia da Polimerase
Densidade Demográfica
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1510
[Cu] Atualização por classe:150904
[Lr] Data última revisão:
150904
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150904
[St] Status:MEDLINE
[do] DOI:10.1093/jme/tjv025


  10 / 161 MEDLINE  
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[PMID]:26175029
[Au] Autor:Harris MC; Yang F; Jackson DM; Dotseth EJ; Paulson SL; Hawley DM
[Ad] Endereço:Department of Biological Sciences, Virginia Tech, Blacksburg, Virginia; Department of Entomology, Virginia Tech, Blacksburg, Virginia; Division of Infectious Disease Epidemiology, Office of Epidemiology and Prevention Services, West Virginia Department of Health and Human Resources, Charleston, West
[Ti] Título:La Crosse Virus Field Detection and Vector Competence of Culex Mosquitoes.
[So] Source:Am J Trop Med Hyg;93(3):461-7, 2015 Sep.
[Is] ISSN:1476-1645
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:La Crosse virus (LACV), a leading cause of arboviral pediatric encephalitis in the United States, is emerging in Appalachia. Here, we report field and laboratory evidence that suggest LACV may be using Culex mosquitoes as additional vectors in this region. This bunyavirus was detected by reverse-transcriptase polymerase chain reaction in two pools of Culex mosquitoes in southwestern Virginia and in six pools in West Virginia. To assess vector competence, we offered LACV blood meals to field-collected Culex restuans Theobald, Cx. pipiens L., and Aedes triseriatus (Say). Both Culex species were susceptible to infection. LACV-positive salivary expectorate, indicative of the ability to transmit, was detected in a small proportion of Cx. restuans (9%) and Cx. pipiens (4%) compared with Ae. triseriatus (40%). In a companion study of Cx. restuans only, we found that adults derived from nutritionally stressed larvae were significantly more likely to disseminate and transmit LACV. Our results indicate a potential role of Culex spp. in LACV dynamics that should be explored further in endemic areas.
[Mh] Termos MeSH primário: Culex/virologia
Encefalite da Califórnia/transmissão
Insetos Vetores/virologia
Vírus La Crosse/fisiologia
[Mh] Termos MeSH secundário: Aedes/virologia
Animais
Feminino
Vigilância da População
RNA Viral/isolamento & purificação
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Virginia
West Virginia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:150716
[St] Status:MEDLINE
[do] DOI:10.4269/ajtmh.14-0128



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