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Pesquisa : B04.820.095 [Categoria DeCS]
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[PMID]:28941906
[Au] Autor:Yang Z; Chambers H; DiCaprio E; Gao G; Li J
[Ad] Endereço:Department of Biology, College of Life Science, Huzhou University, Huzhou, Zhejiang, China.
[Ti] Título:Internalization and dissemination of human norovirus and Tulane virus in fresh produce is plant dependent.
[So] Source:Food Microbiol;69:25-32, 2018 Feb.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Human norovirus (NoV) is a leading cause of fresh produce associated outbreaks. Previous research indicates that the roots of growing leafy greens and berries internalize human NoV. However the effect of plant type and inoculum level on internalization rates has not been directly compared. In this study we compared the internalization and dissemination rates of human NoV and its surrogate, Tulane virus (TV) in green onion, radishes, and Romaine lettuce. We also evaluated the effect inoculum level and plant growth matrix on the rate of viral internalization. In the hydroponic growth system, we detected internalization and dissemination of human NoV RNA in green onions. In hydroponically growing green onions inoculated with high titer TV, we found higher rates of internalization and dissemination compared to green onions inoculated with low titer TV. In soil growth systems, no infectious TV was detected in either green onion or radishes. However, in Romaine lettuce plants grown in soil approximately 4 log PFU/g was recovered from all tissues on day 14 p.i. Overall, we found that the type of plant, growth matrix, and the inoculum level influences the internalization and dissemination of human NoV and TV.
[Mh] Termos MeSH primário: Caliciviridae/fisiologia
Contaminação de Alimentos/análise
Alface/virologia
Norovirus/fisiologia
Cebolas/virologia
Raphanus/virologia
Verduras/virologia
Internalização do Vírus
[Mh] Termos MeSH secundário: Caliciviridae/genética
Caliciviridae/isolamento & purificação
Seres Humanos
Alface/crescimento & desenvolvimento
Norovirus/genética
Norovirus/isolamento & purificação
Cebolas/crescimento & desenvolvimento
Raphanus/crescimento & desenvolvimento
Microbiologia do Solo
Verduras/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170925
[St] Status:MEDLINE


  2 / 714 MEDLINE  
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[PMID]:28815386
[Au] Autor:Mor SK; Phelps NBD; Ng TFF; Subramaniam K; Primus A; Armien AG; McCann R; Puzach C; Waltzek TB; Goyal SM
[Ad] Endereço:Minnesota Veterinary Diagnostic Laboratory, Department of Veterinary Population Medicine, University of Minnesota, 1333 Gortner Avenue, St. Paul, MN, 55108, USA. kumars@umn.edu.
[Ti] Título:Genomic characterization of a novel calicivirus, FHMCV-2012, from baitfish in the USA.
[So] Source:Arch Virol;162(12):3619-3627, 2017 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:During regulatory sampling of fathead minnows (Pimephales promelas), a novel calicivirus was isolated from homogenates of kidney and spleen inoculated into bluegill fry (BF-2) cells. Infected cell cultures exhibiting cytopathic effects were screened by PCR-based methods for selected fish viral pathogens. Illumina HiSeq next generation sequencing of the total RNA revealed a novel calicivirus genome that showed limited protein sequence similarity to known homologs in a BLASTp search. The complete genome of this fathead minnow calicivirus (FHMCV) is 6564 nt long, encoding a polyprotein of 2114 aa in length. The complete polyprotein shared only 21% identity with Atlantic salmon calicivirus,followed by 11% to 14% identity with mammalian caliciviruses. A molecular detection assay (RT-PCR) was designed from this sequence for screening of field samples for FHMCV in the future. This virus likely represents a prototype species of a novel genus in the family Caliciviridae, tentatively named "Minovirus".
[Mh] Termos MeSH primário: Infecções por Caliciviridae/veterinária
Caliciviridae/classificação
Caliciviridae/isolamento & purificação
Cyprinidae/virologia
Genoma Viral
Filogenia
[Mh] Termos MeSH secundário: Estruturas Animais/virologia
Animais
Caliciviridae/genética
Infecções por Caliciviridae/virologia
Células Cultivadas
Efeito Citopatogênico Viral
Genômica
Rim/virologia
Reação em Cadeia da Polimerase
RNA Viral/genética
Análise de Sequência de DNA
Homologia de Sequência
Baço/virologia
Estados Unidos
Proteínas Virais/genética
Cultura de Vírus
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral); 0 (Viral Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170818
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3519-6


  3 / 714 MEDLINE  
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[PMID]:28530548
[Au] Autor:Chhabra P; Ranjan P; Cromeans T; Sambhara S; Vinjé J
[Ad] Endereço:1​Gastroenteritis and Respiratory Viruses Laboratory Branch, Division of Viral Diseases, National Center for Immunizations and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, GA, 30329, USA.
[Ti] Título:Critical role of RIG-I and MDA5 in early and late stages of Tulane virus infection.
[So] Source:J Gen Virol;98(5):1016-1026, 2017 May.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Human noroviruses are a major cause of acute gastroenteritis worldwide, but the lack of a robust cell culture system or small animal model have hampered a better understanding of innate immunity against these viruses. Tulane virus (TV) is the prototype virus of a tentative new genus, Recovirus, in the family Caliciviridae. Its epidemiology and biological properties most closely resemble human norovirus. The host innate immune response to RNA virus infection primarily involves pathogen-sensing toll-like receptors (TLRs) TLR3 and TLR7 and retinoic acid-inducible gene I-like receptor RIG-I and melanoma differentiation associated gene 5 (MDA5). In this study, by using siRNA knockdown, we report that TV infection in LLC-MK2 cells results in an early [3 h post infection (h p.i.), P<0.05] RIG-I-dependent and type I interferon-mediated antiviral response, whereas an MDA5-mediated antiviral effect was observed at later (12 h p.i.; P<0.05) stages of TV replication. Induction of RIG-I and MDA5 was critical for inhibition of TV replication. Furthermore, pre-activation of the RIG-I/MDA5 pathway prevented TV replication (>900-fold decrease; P<0.05), suggesting that RIG-I and MDA5 ligands could be used to develop novel preventive and therapeutic measures against norovirus.
[Mh] Termos MeSH primário: Infecções por Caliciviridae/imunologia
Caliciviridae/imunologia
Proteína DEAD-box 58/metabolismo
Interações Hospedeiro-Patógeno
Imunidade Inata
Helicase IFIH1 Induzida por Interferon/metabolismo
Replicação Viral
[Mh] Termos MeSH secundário: Animais
Técnicas de Silenciamento de Genes
Macaca mulatta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.6.4.13 (DEAD Box Protein 58); EC 3.6.4.13 (Interferon-Induced Helicase, IFIH1)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170523
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000769


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[PMID]:28289976
[Au] Autor:Wang F; Wang M; Dong Y; Zhang B; Zhang D
[Ad] Endereço:Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, People's Republic of China.
[Ti] Título:Genetic characterization of a novel calicivirus from a goose.
[So] Source:Arch Virol;162(7):2115-2118, 2017 Jul.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:A novel calicivirus (strain H146) was detected in a goose and sequenced. The H146 genome consisted of two open reading frames (ORFs) with an 8-nucleotide (nt) overlap between the two ORFs, similar to what has been found in the bat sapovirus TLC58. The virus was most closely related to nacoviruses when comparing the complete genome sequence (49% identity), non-structural region (NS; 31-34% amino acid [aa] sequence identity), and major structural VP1 region (28-30% aa identity), whereas both goose calicivirus N and feline calicivirus were the closest relatives of H146 in the VP2 region (20% aa sequence identity). The levels of divergence between H146 and its closest relatives in different genomic regions are comparable to those between some members of different genera. Phylogenetic analysis based on the NS and VP1 amino acid sequences clearly demonstrated that H146 formed a separate clade. Thus, calicivirus H146 was identified as a founding member of a novel genus for which we propose the name "Sanovirus".
[Mh] Termos MeSH primário: Caliciviridae/classificação
Gansos/virologia
Filogenia
[Mh] Termos MeSH secundário: Animais
Caliciviridae/genética
Caliciviridae/isolamento & purificação
Genoma Viral
Fases de Leitura Aberta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170315
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3302-8


  5 / 714 MEDLINE  
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[PMID]:28040514
[Au] Autor:Bennett S; Gunson RN
[Ad] Endereço:West of Scotland Specialist Virology Centre, United Kingdom. Electronic address: susan.bennett@ggc.scot.nhs.uk.
[Ti] Título:The development of a multiplex real-time RT-PCR for the detection of adenovirus, astrovirus, rotavirus and sapovirus from stool samples.
[So] Source:J Virol Methods;242:30-34, 2017 Apr.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Viral gastroenteritis is a major health problem with significant morbidity and economic consequences. Viral gastroenteritis is caused by a number of viruses, including norovirus, rotavirus, adenovirus, astrovirus, and sapovirus. Conventional diagnosis is based on direct antigen detection and electron microscopy, however enzyme immunoassay's are insensitive and not available for all relevant pathogens, and electron microscope (EM) is no longer routinely carried out in most laboratories. Most laboratories now offer norovirus real-time PCR testing however the availability of other assays is variable. Commercial methods for the detection of inflectional intestinal disease (IID) are available but these can be expensive and are not commonly used. This paper describes the development of a single multiplex assay for the simultaneous detection of adenovirus, astrovirus, rotavirus and sapovirus from stool samples. The multiplex was evaluated by assessing endpoint sensitivity, specificity, panel of clinical samples, quality control (QC) panel and the robustness and reproducibility of the multiplex.
[Mh] Termos MeSH primário: Adenovírus Humanos/isolamento & purificação
Caliciviridae/isolamento & purificação
Fezes/virologia
Gastroenterite/diagnóstico
Mamastrovirus/isolamento & purificação
Reação em Cadeia da Polimerase Multiplex/métodos
Rotavirus/isolamento & purificação
[Mh] Termos MeSH secundário: Infecções por Adenovirus Humanos/diagnóstico
Infecções por Adenovirus Humanos/virologia
Adenovírus Humanos/genética
Caliciviridae/genética
Infecções por Caliciviridae/diagnóstico
Infecções por Caliciviridae/virologia
Primers do DNA
Sondas de DNA
Gastroenterite/virologia
Seres Humanos
Técnicas Imunoenzimáticas
Limite de Detecção
Mamastrovirus/genética
Norovirus/genética
Norovirus/isolamento & purificação
Reação em Cadeia da Polimerase em Tempo Real
Reprodutibilidade dos Testes
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Rotavirus/genética
Infecções por Rotavirus/diagnóstico
Infecções por Rotavirus/virologia
Sapovirus/genética
Sapovirus/isolamento & purificação
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA Probes)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170102
[St] Status:MEDLINE


  6 / 714 MEDLINE  
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[PMID]:28040155
[Au] Autor:Lacombe A; Niemira BA; Gurtler JB; Sites J; Boyd G; Kingsley DH; Li X; Chen H
[Ad] Endereço:National College of Natural Medicine, 014 SE Porter St., Portland, OR 97201, USA.
[Ti] Título:Nonthermal inactivation of norovirus surrogates on blueberries using atmospheric cold plasma.
[So] Source:Food Microbiol;63:1-5, 2017 May.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Viruses are currently the leading cause of foodborne outbreaks, most of which are associated with foods consumed raw. Cold plasma (CP) is an emerging novel nonthermal technology that can be used to surface decontaminate foods. This study investigated CP technology for the nonthermal inactivation of human norovirus surrogates, Tulane virus (TV) and murine norovirus (MNV), on the surface of blueberries. Blueberries (5 g) were weighed into sterile 4 oz. glass jars and inoculated with TV, 5 log PFU/g. Samples were treated with atmospheric CP for 0, 15, 30, 45, and 60 s at a working distance of 7.5 cm with 4 cubic feet/minute (cfm) of CP jet. Temperature readings were taken with an infrared camera prior to, and immediately following, CP treatments. In order to establish the impact of air flow during CP treatment (4 cfm), an additional 7 cfm jet of room temperature air was introduced from a separate nozzle. The experiment was repeated with 90 and 120 s as additional treatment time points. Viral titers were measured immediately after each treatment with a plaque assay using LLC-MK2 cells (TV) or RAW 264.7 cells (MNV). TV was significantly reduced 1.5 PFU/g compared to the control after treatment time of 45s, which was achieved regardless of temperature conditions. With the addition of 7 cfm of ambient air, the maximum log reduction for TV was 3.5 log PFU/g after 120s of treatment. MNV was significantly reduced by 0.5 log PFU/g compare to the control at 15s, and further treatment of MNV with ambient air brought the log reduction to greater than 5 log PFU/g at 90 s of treatment (Fig. 3). These results demonstrate that CP viral inactivation does not rely on thermal inactivation, and is therefore nonthermal in nature. With further optimization, CP may be used by food processors as a means of nonthermal inactivation of foodborne viruses.
[Mh] Termos MeSH primário: Mirtilos Azuis (Planta)/virologia
Caliciviridae/fisiologia
Norovirus/fisiologia
Gases em Plasma
Temperatura Ambiente
Inativação de Vírus
[Mh] Termos MeSH secundário: Animais
Microbiologia de Alimentos
Inocuidade dos Alimentos/métodos
Seres Humanos
Camundongos
Ensaio de Placa Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plasma Gases)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170307
[Lr] Data última revisão:
170307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170102
[St] Status:MEDLINE


  7 / 714 MEDLINE  
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[PMID]:27756911
[Au] Autor:Ohba M; Oka T; Ando T; Arahata S; Ikegaya A; Takagi H; Ogo N; Zhu C; Owada K; Kawamori F; Wang Q; Saif LJ; Asai A
[Ad] Endereço:Center for Drug Discovery, Graduate School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan.
[Ti] Título:Antiviral effect of theaflavins against caliciviruses.
[So] Source:J Antibiot (Tokyo);70(4):443-447, 2017 Apr.
[Is] ISSN:0021-8820
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Caliciviruses are contagious pathogens of humans and various animals. They are the most common cause of viral gastroenteritis in humans, and can cause lethal diseases in domestic animals such as cats, rabbits and immunocompromised mice. In this study, we conducted cytopathic effect-based screening of 2080 selected compounds from our in-house library to find antiviral compounds against three culturable caliciviruses: feline calicivirus, murine norovirus (MNV) and porcine sapovirus (PoSaV). We identified active six compounds, of which two compounds, both related to theaflavins, showed broad antiviral activities against all three caliciviruses; three compounds (abamectin, a mixture of avermectin B1a and B1b; avermectin B1a; and (-)-epigallocatechin gallate hydrate) were effective against PoSaV only; and a heterocyclic carboxamide derivative (BFTC) specifically inhibited MNV infectivity in cell cultures. Further studies of the antiviral mechanism and structure-activity relationship of theaflavins suggested the following: (1) theaflavins worked before the viral entry step; (2) the effect of theaflavins was time- and concentration-dependent; and (3) the hydroxyl groups of the benzocycloheptenone ring were probably important for the anti-calicivirus activity of theaflavins. Theaflavins could be used for the calicivirus research, and as potential disinfectants and antiviral reagents to prevent and control calicivirus infections in animals and humans.
[Mh] Termos MeSH primário: Antivirais/farmacologia
Biflavonoides/farmacologia
Caliciviridae/efeitos dos fármacos
Catequina/farmacologia
Flavinas/farmacologia
[Mh] Termos MeSH secundário: Animais
Infecções por Caliciviridae
Calicivirus Felino/efeitos dos fármacos
Catequina/análogos & derivados
Gatos
Efeito Citopatogênico Viral/efeitos dos fármacos
Avaliação Pré-Clínica de Medicamentos
Seres Humanos
Ivermectina/análogos & derivados
Ivermectina/farmacologia
Camundongos
Norovirus/efeitos dos fármacos
Estrutura Quaternária de Proteína
Sapovirus/efeitos dos fármacos
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Biflavonoids); 0 (Flavins); 1IA46M0D13 (theaflavin); 5U8924T11H (abamectin); 70288-86-7 (Ivermectin); 73989-17-0 (avermectin); 8R1V1STN48 (Catechin); BQM438CTEL (epigallocatechin gallate)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170504
[Lr] Data última revisão:
170504
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161021
[St] Status:MEDLINE
[do] DOI:10.1038/ja.2016.128


  8 / 714 MEDLINE  
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[PMID]:27697158
[Au] Autor:Huang Y; Ye M; Cao X; Chen H
[Ad] Endereço:Department of Animal and Food Sciences, University of Delaware, Newark, DE 19716-2150, USA.
[Ti] Título:Pulsed light inactivation of murine norovirus, Tulane virus, Escherichia coli O157:H7 and Salmonella in suspension and on berry surfaces.
[So] Source:Food Microbiol;61:1-4, 2017 Feb.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pulsed light (PL) inactivation of two human norovirus (HuNoV) surrogates, murine norovirus (MNV-1) and Tulane virus (TV), and two bacterial pathogens, Escherichia coli O157:H7 and Salmonella, were evaluated. The viruses and bacteria were suspended in phosphate buffered saline (PBS) to final populations of ∼6 log PFU/mL and ∼6 log CFU/mL, respectively. Both viral and bacterial suspensions were then irradiated by PL for different durations and the reductions of each microorganisms were determined. MNV-1 and TV were significantly (P < 0.05) more resistant to PL treatment than Salmonella and E. coli O157:H7 in PBS suspension. MNV-1, Salmonella and E. coli O157:H7 were also inoculated on strawberries and blueberries and the PL inactivation of each microorganism was determined. Lower inactivation of each microorganism was achieved on berry surfaces than in PBS suspension. This study shows that PL can induce rapid inactivation of MNV-1, TV, Salmonella and E. coli O157:H7 in clear suspension with viruses more resistant to PL treatment than bacteria. The efficacy of PL treatment is substantially influenced by food surface structure.
[Mh] Termos MeSH primário: Caliciviridae/efeitos da radiação
Escherichia coli O157/efeitos da radiação
Frutas/microbiologia
Luz
Viabilidade Microbiana
Norovirus/efeitos da radiação
Salmonella/efeitos da radiação
[Mh] Termos MeSH secundário: Animais
Mirtilos Azuis (Planta)/microbiologia
Mirtilos Azuis (Planta)/virologia
Microbiologia de Alimentos
Fragaria/microbiologia
Fragaria/virologia
Frutas/virologia
Seres Humanos
Camundongos
Suspensões
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Suspensions)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161005
[St] Status:MEDLINE


  9 / 714 MEDLINE  
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[PMID]:26965677
[Ti] Título:Clinical, pathological, immunohistochemical and molecular characterization of feline chronic gingivostomatitis.
[So] Source:J Feline Med Surg;19(4):NP1, 2017 Apr.
[Is] ISSN:1532-2750
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Veronica Machado Rolim, Saulo Petinatti Pavarini, Fabrício Souza Campos, Viviam Pignone, Cláudia Faraco, Marcelo de Souza Muccillo, Paulo Michel Roehe, Fernanda Viera Amorim da Costa, and David Driemeier J Feline Med Surg. Epub ahead of print 8 February 2016. DOI: 10.1177/1098612X16628578.
[Mh] Termos MeSH primário: Doenças do Gato/diagnóstico
Gengivite/veterinária
[Mh] Termos MeSH secundário: Animais
Caliciviridae/isolamento & purificação
Doenças do Gato/patologia
Doenças do Gato/virologia
Gatos
DNA Viral/análise
Feminino
Gengivite/diagnóstico
Imuno-Histoquímica/veterinária
Vírus da Leucemia Felina/isolamento & purificação
Masculino
Reação em Cadeia da Polimerase/veterinária
Estomatite/diagnóstico
Estomatite/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160312
[St] Status:MEDLINE
[do] DOI:10.1177/1098612X16639004


  10 / 714 MEDLINE  
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Roehe, Paulo Michel
Texto completo
[PMID]:26858258
[Au] Autor:Rolim VM; Pavarini SP; Campos FS; Pignone V; Faraco C; Muccillo MS; Roehe PM; da Costa FV; Driemeier D
[Ad] Endereço:1 Department of Veterinary Pathology of the Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
[Ti] Título:Clinical, pathological, immunohistochemical and molecular characterization of feline chronic gingivostomatitis.
[So] Source:J Feline Med Surg;19(4):403-409, 2017 Apr.
[Is] ISSN:1532-2750
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Objectives This study presents the clinical, pathological, immunohistochemical and molecular characterization of 26 cats with feline chronic gingivostomatitis (FCG). Methods Oral mucosal biopsies, blood and swabs were collected from cats presenting with oral lesions. The tissue sections were submitted for histopathology and immunohistochemical analysis for feline calicivirus (FCV), feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV). The swabs were subjected to PCR analysis for FCV, and blood for FeLV and FIV. Results The main clinical findings were dysphagia (88.2%), halitosis (76.5%), sialorrhea (47.1%), weight loss (41.2%), intense oral discomfort (35.3%), oral hemorrhage (17.6%), and lackluster and fragile coat (11.8%). Gross inspection revealed bilateral lesions across the palatoglossal fold to the lateral tongue base. The lesions were diffuse, proliferative, intensely red and friable, and bled easily upon examination in 80.8% of cases. In 23.1% of cases, the lesions were multifocal to coalescent, at times forming multiple vesicles on a reddened, edematous palatoglossal fold. Microscopic examination showed that 15.4% of lesions had moderate (grade 2) and 84.6% had severe (grade 3) inflammation. Immunohistochemistry revealed the presence of FeLV antigens in the epithelium and the inflammatory infiltrate of 30.8% of the cats with FCG. FCV antigens were not detected in the FCG lesions. Conclusions and relevance The FCG cases analyzed could not be correlated with FCV. It is possible that FeLV plays a role as a causal agent of lesions in cases where the presence of the virus has been confirmed by immunohistochemistry in epithelial samples.
[Mh] Termos MeSH primário: Doenças do Gato/diagnóstico
Gengivite/veterinária
Estomatite/veterinária
[Mh] Termos MeSH secundário: Animais
Caliciviridae/isolamento & purificação
Doenças do Gato/sangue
Doenças do Gato/patologia
Doenças do Gato/virologia
Gatos
Feminino
Gengivite/diagnóstico
Vírus da Imunodeficiência Felina/isolamento & purificação
Imuno-Histoquímica/veterinária
Vírus da Leucemia Felina/isolamento & purificação
Masculino
Reação em Cadeia da Polimerase/veterinária
Estomatite/diagnóstico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160210
[St] Status:MEDLINE
[do] DOI:10.1177/1098612X16628578



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