Base de dados : MEDLINE
Pesquisa : B04.820.095.500 [Categoria DeCS]
Referências encontradas : 19 [refinar]
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  1 / 19 MEDLINE  
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[PMID]:28714849
[Au] Autor:Le Pendu J; Abrantes J; Bertagnoli S; Guitton JS; Le Gall-Reculé G; Lopes AM; Marchandeau S; Alda F; Almeida T; Célio AP; Bárcena J; Burmakina G; Blanco E; Calvete C; Cavadini P; Cooke B; Dalton K; Delibes Mateos M; Deptula W; Eden JS; Wang F; Ferreira CC; Ferreira P; Foronda P; Gonçalves D; Gavier-Widén D; Hall R; Hukowska-Szematowicz B; Kerr P; Kovaliski J; Lavazza A; Mahar J; Malogolovkin A; Marques RM; Marques S; Martin-Alonso A; Monterroso P; Moreno S; Mutze G; Neimanis A; Niedzwiedzka-Rystwej P; Peacock D; Parra F; Rocchi M; Rouco C; Ruvoën-Clouet N; Silva E; Silvério D; Strive T; Thompson G
[Ad] Endereço:1​CRCINA, Inserm, Université d'Angers, Université de Nantes, Nantes, France.
[Ti] Título:Proposal for a unified classification system and nomenclature of lagoviruses.
[So] Source:J Gen Virol;98(7):1658-1666, 2017 Jul.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Lagoviruses belong to the Caliciviridae family. They were first recognized as highly pathogenic viruses of the European rabbit (Oryctolagus cuniculus) and European brown hare (Lepus europaeus) that emerged in the 1970-1980s, namely, rabbit haemorrhagic disease virus (RHDV) and European brown hare syndrome virus (EBHSV), according to the host species from which they had been first detected. However, the diversity of lagoviruses has recently expanded to include new related viruses with varying pathogenicity, geographic distribution and host ranges. Together with the frequent recombination observed amongst circulating viruses, there is a clear need to establish precise guidelines for classifying and naming lagovirus strains. Therefore, here we propose a new nomenclature based on phylogenetic relationships. In this new nomenclature, a single species of lagovirus would be recognized and called Lagovirus europaeus. The species would be divided into two genogroups that correspond to RHDV- and EBHSV-related viruses, respectively. Genogroups could be subdivided into genotypes, which could themselves be subdivided into phylogenetically well-supported variants. Based on available sequences, pairwise distance cutoffs have been defined, but with the accumulation of new sequences these cutoffs may need to be revised. We propose that an international working group could coordinate the nomenclature of lagoviruses and any proposals for revision.
[Mh] Termos MeSH primário: Lagovirus/classificação
RNA Viral/genética
Terminologia como Assunto
[Mh] Termos MeSH secundário: Animais
Infecções por Caliciviridae/virologia
Genótipo
Lebres
Lagovirus/genética
Lagovirus/patogenicidade
Filogenia
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170804
[Lr] Data última revisão:
170804
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000840


  2 / 19 MEDLINE  
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[PMID]:27902397
[Au] Autor:Conley M; Emmott E; Orton R; Taylor D; Carneiro DG; Murata K; Goodfellow IG; Hansman GS; Bhella D
[Ad] Endereço:1​Medical Research Council - University of Glasgow Centre for Virus Research, Sir Michael Stoker Building, Garscube Campus, 464 Bearsden Road, Glasgow G61 1QH, UK.
[Ti] Título:Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures.
[So] Source:J Gen Virol;98(1):68-76, 2017 Jan.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Vesivirus 2117 is an adventitious agent that, in 2009, was identified as a contaminant of Chinese hamster ovary cells propagated in bioreactors at a pharmaceutical manufacturing plant belonging to Genzyme. The consequent interruption in supply of Fabrazyme and Cerezyme (drugs used to treat Fabry and Gaucher diseases, respectively) caused significant economic losses. Vesivirus 2117 is a member of the Caliciviridae, a family of small icosahedral viruses encoding a positive-sense RNA genome. We have used cryo-electron microscopy and three-dimensional image reconstruction to calculate a structure of vesivirus 2117 virus-like particles as well as feline calicivirus and a chimeric sapovirus. We present a structural comparison of several members of the Caliciviridae, showing that the distal P domain of vesivirus 2117 is morphologically distinct from that seen in other known vesivirus structures. Furthermore, at intermediate resolutions, we found a high level of structural similarity between vesivirus 2117 and Caliciviridae from other genera: sapovirus and rabbit hemorrhagic disease virus. Phylogenetic analysis confirms vesivirus 2117 as a vesivirus closely related to canine vesiviruses. We postulate that morphological differences in virion structure seen between vesivirus clades may reflect differences in receptor usage.
[Mh] Termos MeSH primário: Capsídeo/ultraestrutura
Lagovirus/ultraestrutura
Sapovirus/ultraestrutura
Vesivirus/ultraestrutura
[Mh] Termos MeSH secundário: Animais
Cricetulus
Microscopia Crioeletrônica
Imagem Tridimensional
Filogenia
RNA Viral/genética
Análise de Sequência de DNA
Vesivirus/genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000658


  3 / 19 MEDLINE  
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[PMID]:26540662
[Au] Autor:Esteves PJ; Abrantes J; Bertagnoli S; Cavadini P; Gavier-Widén D; Guitton JS; Lavazza A; Lemaitre E; Letty J; Lopes AM; Neimanis AS; Ruvoën-Clouet N; Le Pendu J; Marchandeau S; Le Gall-Reculé G
[Ad] Endereço:InBIO-Research Network in Biodiversity and Evolutionary Biology, CIBIO, Campus de Vairão, Universidade do Porto, Vairão, Portugal.
[Ti] Título:Emergence of Pathogenicity in Lagoviruses: Evolution from Pre-existing Nonpathogenic Strains or through a Species Jump?
[So] Source:PLoS Pathog;11(11):e1005087, 2015.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Evolução Biológica
Genoma Bacteriano/genética
Lagovirus/isolamento & purificação
Virulência/genética
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Em] Mês de entrada:1604
[Cu] Atualização por classe:151116
[Lr] Data última revisão:
151116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1005087


  4 / 19 MEDLINE  
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[PMID]:26403184
[Au] Autor:Bárcena J; Guerra B; Angulo I; González J; Valcárcel F; Mata CP; Castón JR; Blanco E; Alejo A
[Ad] Endereço:Centro de Investigación en Sanidad Animal (INIA-CISA), Valdeolmos, Madrid, Spain. barcena@inia.es.
[Ti] Título:Comparative analysis of rabbit hemorrhagic disease virus (RHDV) and new RHDV2 virus antigenicity, using specific virus-like particles.
[So] Source:Vet Res;46:106, 2015 Sep 24.
[Is] ISSN:1297-9716
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In 2010 a new Lagovirus related to rabbit haemorrhagic disease virus (RHDV) emerged in France and has since rapidly spread throughout domestic and wild rabbit populations of several European countries. The new virus, termed RHDV2, exhibits distinctive genetic, antigenic and pathogenic features. Notably, RHDV2 kills rabbits previously vaccinated with RHDV vaccines. Here we report for the first time the generation and characterization of RHDV2-specific virus-like particles (VLPs). Our results further confirmed the differential antigenic properties exhibited by RHDV and RHDV2, highlighting the need of using RHDV2-specific diagnostic assays to monitor the spread of this new virus.
[Mh] Termos MeSH primário: Antígenos Virais/imunologia
Infecções por Caliciviridae/veterinária
Lagovirus/imunologia
Coelhos
[Mh] Termos MeSH secundário: Animais
Infecções por Caliciviridae/imunologia
Infecções por Caliciviridae/virologia
Vírus da Doença Hemorrágica de Coelhos/genética
Vírus da Doença Hemorrágica de Coelhos/imunologia
Lagovirus/genética
Filogenia
Análise de Sequência de DNA/veterinária
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, Viral)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:150929
[Lr] Data última revisão:
150929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150926
[St] Status:MEDLINE
[do] DOI:10.1186/s13567-015-0245-5


  5 / 19 MEDLINE  
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[PMID]:25828691
[Au] Autor:Lavazza A; Cavadini P; Barbieri I; Tizzani P; Pinheiro A; Abrantes J; Esteves PJ; Grilli G; Gioia E; Zanoni M; Meneguz P; Guitton JS; Marchandeau S; Chiari M; Capucci L
[Ti] Título:Field and experimental data indicate that the eastern cottontail (Sylvilagus floridanus) is susceptible to infection with European brown hare syndrome (EBHS) virus and not with rabbit haemorrhagic disease (RHD) virus.
[So] Source:Vet Res;46:13, 2015 Feb 24.
[Is] ISSN:1297-9716
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The eastern cottontail (Sylvilagus floridanus) is an American lagomorph. In 1966, it was introduced to Italy, where it is currently widespread. Its ecological niche is similar to those of native rabbits and hares and increasing overlap in distribution brings these species into ever closer contact. Therefore, cottontails are at risk of infection with the two lagoviruses endemically present in Italy: Rabbit Haemorrhagic Disease virus (RHDV) and European Brown Hare Syndrome Virus (EBHSV). To verify the susceptibility of Sylvilagus to these viruses, we analyzed 471 sera and 108 individuals from cottontail populations in 9 provinces of north-central Italy from 1999 to 2012. In total, 15-20% of the cottontails tested seropositive for EBHSV; most titres were low, but some were as high as 1/1280. All the cottontails virologically tested for RHDV and EBHSV were negative with the exception of one individual found dead with hares during a natural EBHS outbreak in December 2009. The cottontail and the hares showed typical EBHS lesions, and the EBHSV strain identified was the same in both species (99.9% identity). To experimentally confirm the diagnosis, we performed two trials in which we infected cottontails with both EBHSV and RHDV. One out of four cottontails infected with EBHSV died of an EBHS-like disease, and the three surviving animals developed high EBHSV antibody titres. In contrast, neither mortality nor seroconversion was detected after infection with RHDV. Taken together, these results suggest that Sylvilagus is susceptible to EBHSV infection, which occasionally evolves to EBHS-like disease; the eastern cottontail could therefore be considered a "spill over" or "dead end" host for EBHSV unless further evidence is found to confirm that it plays an active role in the epidemiology of EBHSV.
[Mh] Termos MeSH primário: Infecções por Caliciviridae/veterinária
Surtos de Doenças/veterinária
Lagomorpha
Lagovirus/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/sangue
Infecções por Caliciviridae/epidemiologia
Infecções por Caliciviridae/transmissão
Infecções por Caliciviridae/virologia
Ensaio de Imunoadsorção Enzimática/veterinária
Feminino
Vírus da Doença Hemorrágica de Coelhos/isolamento & purificação
Espécies Introduzidas
Itália/epidemiologia
Masculino
Estações do Ano
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:150404
[Lr] Data última revisão:
150404
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150402
[St] Status:MEDLINE
[do] DOI:10.1186/s13567-015-0149-4


  6 / 19 MEDLINE  
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[PMID]:25155199
[Au] Autor:Lopes AM; Capucci L; Gavier-Widén D; Le Gall-Reculé G; Brocchi E; Barbieri I; Quéméner A; Le Pendu J; Geoghegan JL; Holmes EC; Esteves PJ; Abrantes J
[Ad] Endereço:CIBIO-UP, Centro de Investigação em Biodiversidade e Recursos Genéticos-Universidade do Porto/InBIO, Laboratório Associado, Vairão, Portugal; Departamento de Biologia, Faculdade de Ciências da Universidade do Porto, Porto, Portugal; INSERM, UMR892, CNRS, UMR6299, Université de Nantes, Nantes, France
[Ti] Título:Molecular evolution and antigenic variation of European brown hare syndrome virus (EBHSV).
[So] Source:Virology;468-470:104-12, 2014 Nov.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:European brown hare syndrome virus (EBHSV) is the aetiological agent of European brown hare syndrome (EBHS), a disease affecting Lepus europaeus and Lepus timidus first diagnosed in Sweden in 1980. To characterize EBHSV evolution we studied hare samples collected in Sweden between 1982 and 2008. Our molecular clock dating is compatible with EBHSV emergence in the 1970s. Phylogenetic analysis revealed two lineages: Group A persisted until 1989 when it apparently suffered extinction; Group B emerged in the mid-1980s and contains the most recent strains. Antigenic differences exist between groups, with loss of reactivity of some MAbs over time, which are associated with amino acid substitutions in recognized epitopes. A role for immune selection is also supported by the presence of positively selected codons in exposed regions of the capsid. Hence, EBHSV evolution is characterized by replacement of Group A by Group B viruses, suggesting that the latter possess a selective advantage.
[Mh] Termos MeSH primário: Antígenos Virais/metabolismo
Evolução Molecular
Variação Genética
Lagovirus/genética
[Mh] Termos MeSH secundário: Animais
Anticorpos Monoclonais
Anticorpos Antivirais
Antígenos Virais/genética
Infecções por Bunyaviridae/epidemiologia
Infecções por Bunyaviridae/veterinária
Infecções por Bunyaviridae/virologia
Epitopos/genética
Regulação Viral da Expressão Gênica/fisiologia
Lebres/virologia
Fígado/virologia
Modelos Moleculares
Filogenia
Conformação Proteica
Suécia/epidemiologia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Antibodies, Viral); 0 (Antigens, Viral); 0 (Epitopes)
[Em] Mês de entrada:1502
[Cu] Atualização por classe:141202
[Lr] Data última revisão:
141202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140827
[St] Status:MEDLINE


  7 / 19 MEDLINE  
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[PMID]:24988863
[Au] Autor:Kwit E; Chrobocinska M; Gradzki Z; Jarosz L; Majer-Dziedzic B; Bigoraj E
[Ti] Título:The genetic analysis of new Polish strains of European brown hare syndrome virus.
[So] Source:Pol J Vet Sci;17(2):353-5, 2014.
[Is] ISSN:1505-1773
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In this paper we describe recently occurring outbreaks of European brown hare syndrome (EBHS) in a captive hare population. The aim of our study was to evaluate the phylogenetic position of detected Polish strains compared to other European strains of EBHSV. Investigations were undertaken in hares from different provinces of Poland. Liver or spleen samples were tested for viral RNA using the RT-nested PCR method and the products were subsequently sequenced. The genetic analysis was based on the fragment of gene encoding viral capsid protein; it revealed a high homology and close relationship between Polish and European EBHSV strains isolated between 2001 and 2011.
[Mh] Termos MeSH primário: Infecções por Bunyaviridae/veterinária
Lebres
Lagovirus/genética
[Mh] Termos MeSH secundário: Animais
Infecções por Bunyaviridae/epidemiologia
Infecções por Bunyaviridae/virologia
Lagovirus/isolamento & purificação
Filogenia
Polônia/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1407
[Cu] Atualização por classe:140703
[Lr] Data última revisão:
140703
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140704
[St] Status:MEDLINE


  8 / 19 MEDLINE  
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[PMID]:24069299
[Au] Autor:Iacovakis C; Mamuris Z; Moutou KA; Touloudi A; Hammer AS; Valiakos G; Giannoulis T; Stamatis C; Spyrou V; Athanasiou LV; Kantere M; Asferg T; Giannakopoulos A; Salomonsen CM; Bogdanos D; Birtsas P; Petrovska L; Hannant D; Billinis C
[Ad] Endereço:Faculty of Veterinary Medicine, University of Thessaly, Karditsa, Greece ; Institute for Research & Technology-Thessaly, Larissa, Greece.
[Ti] Título:Polarisation of major histocompatibility complex II host genotype with pathogenesis of European Brown Hare syndrome virus.
[So] Source:PLoS One;8(9):e74360, 2013.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A study was conducted in order to determine the occurrence of European Brown Hare Syndrome virus (EBHSV) in Denmark and possible relation between disease pathogenesis and Major Histocompatibility Complex (MHC) host genotype. Liver samples were examined from 170 brown hares (hunted, found sick or dead), collected between 2004 and 2009. Macroscopical and histopathological findings consistent with EBHS were detected in 24 (14.1%) hares; 35 (20.6%) had liver lesions not typical of the syndrome, 50 (29.4%) had lesions in other tissues and 61 (35.9%) had no lesions. Sixty five (38.2%) of 170 samples were found to be EBHSV-positive (RT-PCR, VP60 gene). In order to investigate associations between viral pathogenesis and host genotype, variation within the exon 2 DQA gene of MHC was assessed. DQA exon 2 analysis revealed the occurrence of seven different alleles in Denmark. Consistent with other populations examined so far in Europe, observed heterozygosity of DQA (H o = 0.1180) was lower than expected (H e = 0.5835). The overall variation for both nucleotide and amino acid differences (2.9% and 14.9%, respectively) were lower in Denmark than those assessed in other European countries (8.3% and 16.9%, respectively). Within the peptide binding region codons the number of nonsynonymous substitutions (dN) was much higher than synonymous substitutions (dS), which would be expected for MHC alleles under balancing selection. Allele frequencies did not significantly differ between EBHSV-positive and -negative hares. However, allele Leeu-DQA*30 was detected in significantly higher (P = 0.000006) frequency among the positive hares found dead with severe histopathological lesions than among those found sick or apparently healthy. In contrast, the latter group was characterized by a higher frequency of the allele Leeu-DQA*14 as well as the proportion of heterozygous individuals (P = 0.000006 and P = 0.027). These data reveal a polarisation between EBHSV pathogenesis and MHC class II genotype within the European brown hare in Denmark.
[Mh] Termos MeSH primário: Doenças dos Animais/genética
Infecções por Bunyaviridae/veterinária
Genes MHC Classe II
Genótipo
Lagovirus/classificação
[Mh] Termos MeSH secundário: Alelos
Sequência de Aminoácidos
Doenças dos Animais/epidemiologia
Doenças dos Animais/virologia
Animais
Dinamarca
Éxons
Genes Virais
Predisposição Genética para Doença
Variação Genética
Geografia
Lebres/genética
Lebres/virologia
Lagovirus/genética
Lagovirus/isolamento & purificação
Dados de Sequência Molecular
Filogenia
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1405
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130927
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0074360


  9 / 19 MEDLINE  
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[PMID]:23640583
[Au] Autor:Lopes AM; Gavier-Widén D; Le Gall-Reculé G; Esteves PJ; Abrantes J
[Ad] Endereço:CIBIO-UP, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, InBIO, Laboratório Associado, Campus Agrário de Vairão, Rua Padre Armando Quintas, Vairão, Portugal, analopes@cibio.up.pt.
[Ti] Título:Complete coding sequences of European brown hare syndrome virus (EBHSV) strains isolated in 1982 in Sweden.
[So] Source:Arch Virol;158(10):2193-6, 2013 Oct.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:European brown hare syndrome (EBHS) is characterised by high mortality of European brown hares (Lepus europaeus) and mountain hares (Lepus timidus). European brown hare syndrome virus (EBHSV) and the closely related rabbit haemorrhagic disease virus (RHDV) comprise the genus Lagovirus, family Caliciviridae. In contrast to RHDV, which is well studied, with more than 30 complete genome sequences available, the only complete genome sequence available for EBHSV was obtained from a strain isolated in 1989 in France. EBHS was originally diagnosed in Sweden in 1980. Here, we report the complete coding sequences of two EBHSV strains isolated from European brown hares that died with liver lesions characteristic of EBHS in Sweden in 1982. These sequences represent the oldest complete coding sequences of EBHSV isolated from the original area of virus diagnosis. The genomic organisation is similar to that of the published French sequence. Comparison with this sequence revealed several nucleotide substitutions, corresponding to 6 % divergence. At the amino acid level, the Swedish strains are 2 % different from the French strain. Most amino acid substitutions were located within the major capsid protein VP60, but when considering the amino acid sequence length of each protein, VP10 is the protein with the highest percentage of amino acid differences. The same result was obtained when Swedish strains were compared. This evolutionary pattern has not been described previously for members of the genus Lagovirus.
[Mh] Termos MeSH primário: Infecções por Bunyaviridae/veterinária
Lebres
Lagovirus/classificação
Lagovirus/genética
[Mh] Termos MeSH secundário: Animais
Infecções por Bunyaviridae/epidemiologia
Infecções por Bunyaviridae/virologia
Genoma Viral
Dados de Sequência Molecular
RNA Viral/genética
Suécia/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1311
[Cu] Atualização por classe:130927
[Lr] Data última revisão:
130927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130504
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-013-1714-7


  10 / 19 MEDLINE  
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[PMID]:23261802
[Au] Autor:Hoehn M; Kerr PJ; Strive T
[Ad] Endereço:Commonwealth Scientific and Industrial Research Organisation, Division of Ecosystem Sciences, Australia.
[Ti] Título:In situ hybridisation assay for localisation of rabbit calicivirus Australia-1 (RCV-A1) in European rabbit (Oryctolagus cuniculus) tissues.
[So] Source:J Virol Methods;188(1-2):148-52, 2013 Mar.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Recently, a new lagovirus enzootic in Australian wild rabbits was identified and described as rabbit calicivirus Australia-1 (RCV-A1). Unlike the closely related Rabbit Haemorrhagic Disease Virus (RHDV), which causes fulminant hepatitis and rabbit death, RCV-A1 does not appear to induce any clinical disease. RCV-A1 has been postulated to act as an imperfect natural vaccine to RHDV thus reducing RHDV-induced rabbit mortality, which is detrimental for bio-control of rabbits in Australia. This study was carried out to determine in which cells RCV-A1 replication occurs. An in situ hybridisation (ISH) protocol was developed using a RCV-A1 specific probe to localise the virus in rabbit tissues. The results were compared to those obtained with a quantitative RT-PCR assay that had previously been developed to measure RCV-A1 RNA in rabbit tissues. The histology of the tissues was also examined. ISH showed that virus replication, inferred by the presence of detectable RNA, was limited to a small number of epithelial cells towards the tip of the villi in the duodenum. Quantitative RT-PCR detected RCV-A1 RNA in jejunum, ileum and lymphoid tissue at day 3, 4 and 7 post-infection, but no hybridisation was detected in these tissues.
[Mh] Termos MeSH primário: Infecções por Bunyaviridae/veterinária
Hibridização In Situ
Lagovirus/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Infecções por Bunyaviridae/virologia
Histocitoquímica
Íleo/virologia
Jejuno/virologia
Lagovirus/genética
Tecido Linfoide/virologia
Coelhos
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1308
[Cu] Atualização por classe:130228
[Lr] Data última revisão:
130228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121225
[St] Status:MEDLINE



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