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  1 / 3275 MEDLINE  
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[PMID]:29438433
[Au] Autor:Oka T; Stoltzfus GT; Zhu C; Jung K; Wang Q; Saif LJ
[Ad] Endereço:Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, United States of America.
[Ti] Título:Attempts to grow human noroviruses, a sapovirus, and a bovine norovirus in vitro.
[So] Source:PLoS One;13(2):e0178157, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Noroviruses (NoVs) and Sapoviruses (SaVs) are enteric caliciviruses that have been detected in multiple mammalian species, including humans. Currently, efficient cell culture systems have been established only for murine NoVs and porcine SaV Cowden strain. Establishment of an efficient in vitro cell culture system for other NoVs and SaVs remains challenging; however, human NoV (HuNoV) replication in 3D cultured Caco-2 cells and a clone of Caco-2 cells, C2BBe1, human enteroids and in human B cells has been reported. In this study, we tested various cells and culture conditions to grow HuNoVs and a human SaV (HuSaV) to test the possibility of the propagation in different cells and culture conditions. We also attempted to grow a bovine NoV (BoNoV) in ex vivo organ cultures. We did not observe significant RNA level increases for HuSaV and BoNoV under our test conditions. HuNoV RNA levels increased to a maximum of ~600-fold in long-term Caco-2 cells that were cultured for 1-2 months in multi-well plates and inoculated with HuNoV-positive and bacteria-free human stool suspensions using serum-free medium supplemented with the bile acid, GCDCA. However, this positive result was inconsistent. Our results demonstrated that HuNoVs, BoNoV and HuSaV largely failed to grow in vitro under our test conditions. Our purpose is to share our findings with other researchers with the goal to develop efficient, reproducible simplified and cost-effective culture systems for human and animal NoVs and SaVs in the future.
[Mh] Termos MeSH primário: Norovirus/fisiologia
Sapovirus/fisiologia
[Mh] Termos MeSH secundário: Células CACO-2
Seres Humanos
Técnicas In Vitro
Reação em Cadeia da Polimerase/métodos
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180214
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178157


  2 / 3275 MEDLINE  
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[PMID]:28461205
[Au] Autor:Supadej K; Khamrin P; Kumthip K; Kochjan P; Yodmeeklin A; Ushijima H; Maneekarn N
[Ad] Endereço:Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
[Ti] Título:Wide variety of recombinant strains of norovirus GII in pediatric patients hospitalized with acute gastroenteritis in Thailand during 2005 to 2015.
[So] Source:Infect Genet Evol;52:44-51, 2017 Aug.
[Is] ISSN:1567-7257
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Norovirus (NoV) has been reported as being a common cause of acute gastroenteritis both in children and adults worldwide. Of the many variants, NoV GII.4 is the most predominant genotype. One of the mechanisms that drives the evolution and emergence of new variants of NoV is homologous recombination. This study describes the genetic recombination involved in cases of NoV GII detected in pediatric patients with acute gastroenteritis in Chiang Mai, Thailand during 2005 to 2015. From a total of 1938 stool samples, 3 (0.15%) were positive for NoV GI and 298 (15.38%) were identified as NoV GII. The genotypes detected in this study were GI.6, GI.14, GII.1, GII.2, GII.3, GII.4, GII.6, GII.7, GII.12, GII.13, GII.14, GII.15, GII.16, GII.17, GII.20, and GII.21. The NoV recombinant strains were verified by analysis of the partial sequence of ORF1 (RdRp)/ORF2 (capsid) junction. Phylogenetic analyses of partial ORF1 and ORF2 regions resulted in the identification of 21 (6.98%) NoV recombinant strains. Among these, 9 recombination patterns were detected in this study; GII.Pe/GII.4, GII.Pg/GII.1, GII.Pg/GII.12, GII.P7/GII.6, GII.P7/GII.14, GII.P12/GII.4, GII.P16/GII.2, GII.P16/GII.13, and GII.P21/GII.3. The findings demonstrated the wide variety of recombinant strains of NoV GII strains detected in pediatric patients admitted to the hospitals with acute gastroenteritis in Chiang Mai, Thailand during the past decade.
[Mh] Termos MeSH primário: Infecções por Caliciviridae/diagnóstico
Gastroenterite/virologia
Norovirus/genética
[Mh] Termos MeSH secundário: Criança
Evolução Molecular
Genótipo
Hospitalização
Seres Humanos
Norovirus/classificação
Filogenia
Recombinação Genética
Tailândia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


  3 / 3275 MEDLINE  
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[PMID]:28450084
[Au] Autor:Bodnar L; Lorusso E; Di Martino B; Catella C; Lanave G; Elia G; Bányai K; Buonavoglia C; Martella V
[Ad] Endereço:University Aldo Moro of Bari, Valenzano, Italy.
[Ti] Título:Identification of a novel canine norovirus.
[So] Source:Infect Genet Evol;52:75-81, 2017 Aug.
[Is] ISSN:1567-7257
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:By screening a collection of fecal samples from young dogs from different European countries, noroviruses (NoVs) were found in 13/294 (4.4%) animals with signs of enteritis whilst they were not detected in healthy dogs (0/42). An informative portion of the genome (3.4kb at the 3' end) was generated for four NoV strains. In the capsid protein VP1 region, strains 63.15/2015/ITA and FD53/2007/ITA were genetically related to the canine GVI.2 strain C33/Viseu/2007/PRT (97.4-98.6% nt and 90.3-98.6% aa). Strain FD210/2007/ITA displayed the highest identity to the GVI.1 canine strain Bari/91/2007/ITA (88.0% nt and 95.0% aa). Strain 5010/2009/ITA displayed only 66.6-67.6% nt and 75.5-81.6% aa identities to the GVI.1 canine strains FD210/2007/ITA and Bari/91/2007/ITA and the GVI feline strain M49-1/2012/JPN. Identity to the other canine/feline NoVs strains in the VP1 was lower than 67.6% nt and 62.7% aa. Based on the full-length VP1 amino acid sequence and the criteria proposed for distinction of NoV genotypes, the canine NoV 5010/2009/ITA could represent the prototype of a third GVI genotype, thus providing further evidence for the genetic heterogeneity of NoVs in carnivores.
[Mh] Termos MeSH primário: Infecções por Caliciviridae/veterinária
Doenças do Cão/virologia
Gastroenterite/veterinária
Norovirus/classificação
[Mh] Termos MeSH secundário: Animais
Infecções por Caliciviridae/diagnóstico
Infecções por Caliciviridae/epidemiologia
Doenças do Cão/epidemiologia
Cães
Europa (Continente)/epidemiologia
Evolução Molecular
Fezes/virologia
Gastroenterite/virologia
Genoma Viral
Norovirus/genética
Norovirus/isolamento & purificação
Filogenia
Análise de Sequência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  4 / 3275 MEDLINE  
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[PMID]:28453838
[Au] Autor:Lindesmith LC; Mallory ML; Jones TA; Richardson C; Goodwin RR; Baehner F; Mendelman PM; Bargatze RF; Baric RS
[Ad] Endereço:Department of Epidemiology, University of North Carolina, Chapel Hill, NC, USA.
[Ti] Título:Impact of Pre-exposure History and Host Genetics on Antibody Avidity Following Norovirus Vaccination.
[So] Source:J Infect Dis;215(6):984-991, 2017 03 15.
[Is] ISSN:1537-6613
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Background: Development of high avidity, broadly neutralizing antibodies (Abs) is a priority after vaccination against rapidly evolving, widely disseminated viruses like human norovirus. After vaccination with a multivalent GI.1 and GII.4c norovirus virus-like particle (VLP) vaccine candidate adjuvanted with alum and monophosphoryl lipid A (MPL), blockade Ab titers peaked early, with no increase in titer following a second vaccine dose. Methods: Blockade Ab relative avidity was evaluated by measuring the slope of blockade Ab neutralization curves. Results: Blockade Ab avidity to the GI.1 vaccine component peaked at day 35 (7 days after dose 2). Avidities to heterotypic genogroup I VLPs were not sustained at day 35 after vaccination or GI.1 infection, as measured from archived sera. Only secretor-positive participants maintained high avidity blockade Ab to GI.1 at day 180. Avidity to the GII.4c vaccine component peaked at day 7, remained elevated through day 180, and was not secretor dependent. Avidity to an immunologically novel GII.4 strain VLP correlated with preexisting Ab titer to an ancestral strain Epitope A. Conclusions: Host genetics and pre-exposure history shape norovirus vaccine Ab responses, including blockade Ab avidity. Avidity of potentially neutralizing Ab may be an important metric for evaluating vaccine responses to highly penetrant viruses with cross-reactive serotypes.
[Mh] Termos MeSH primário: Afinidade de Anticorpos
Infecções por Caliciviridae/prevenção & controle
Vacinas de Partículas Semelhantes a Vírus/uso terapêutico
Vacinas Virais/uso terapêutico
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos/administração & dosagem
Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Anticorpos Neutralizantes/sangue
Anticorpos Antivirais/sangue
Infecções por Caliciviridae/genética
Reações Cruzadas
Método Duplo-Cego
Epitopos/imunologia
Feminino
Seres Humanos
Masculino
Meia-Idade
Norovirus
Estados Unidos
Vacinação
Vacinas de Partículas Semelhantes a Vírus/administração & dosagem
Vacinas Virais/administração & dosagem
Adulto Jovem
[Pt] Tipo de publicação:CLINICAL TRIAL, PHASE I; JOURNAL ARTICLE; MULTICENTER STUDY; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Antibodies, Neutralizing); 0 (Antibodies, Viral); 0 (Epitopes); 0 (Vaccines, Virus-Like Particle); 0 (Viral Vaccines)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1093/infdis/jix045


  5 / 3275 MEDLINE  
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[PMID]:28449729
[Au] Autor:Bidalot M; Théry L; Kaplon J; De Rougemont A; Ambert-Balay K
[Ad] Endereço:National Reference Centre for Gastroenteritis Viruses, Laboratory of Biology and Pathology, University Hospital Dijon Bourgogne, Dijon, France.
[Ti] Título:Emergence of new recombinant noroviruses GII.p16-GII.4 and GII.p16-GII.2, France, winter 2016 to 2017.
[So] Source:Euro Surveill;22(15), 2017 Apr 13.
[Is] ISSN:1560-7917
[Cp] País de publicação:Sweden
[La] Idioma:eng
[Ab] Resumo:An early increase in outbreaks of norovirus gastroenteritis characterised at the French National Reference Centre occurred this winter season. They were concurrent with an unusual pattern of circulating strains, with three predominant genotypes: the re-emergent variant GII.P4 2009-GII.4 2012 found in 28% of norovirus outbreaks and two new emergent recombinant strains GII.P16-GII.4 2012 and GII.P16-GII.2 never before observed in France, found in 24% and 14% of norovirus outbreaks, respectively.
[Mh] Termos MeSH primário: Infecções por Caliciviridae/virologia
Doenças Transmissíveis Emergentes/epidemiologia
Doenças Transmissíveis Emergentes/virologia
Gastroenterite/epidemiologia
Gastroenterite/virologia
Norovirus/genética
Estações do Ano
[Mh] Termos MeSH secundário: Infecções por Caliciviridae/epidemiologia
Fezes/virologia
França/epidemiologia
Seres Humanos
Norovirus/isolamento & purificação
Vírus Reordenados/genética
Vírus Reordenados/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  6 / 3275 MEDLINE  
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[PMID]:29054741
[Au] Autor:Sangsriratanakul N; Toyofuku C; Suzuki M; Komura M; Yamada M; Alam MS; Ruenphet S; Shoham D; Sakai K; Takehara K
[Ad] Endereço:Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8, Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan; The United Graduate School of Veterinary Science, Gifu University, 1-1, Yanagido, Gifu 501-1193, Japan.
[Ti] Título:Virucidal efficacy of food additive grade calcium hydroxide against surrogate of human norovirus.
[So] Source:J Virol Methods;251:83-87, 2018 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:An alkaline agent, namely, food additive grade calcium hydroxide (FdCa(OH) ) in the solution, powder and suspension forms was evaluated as a virucidal agent, using a murine norovirus (MNV) as the surrogate for human norovirus. The main constituent of FdCa(OH) is Ca(OH) , which has pH 13 in 0.17% solution. The results showed that 0.17% FdCa(OH) solution could inactivate MNV within 30s even in the presence of organic materials (5% fetal bovine serum (FBS)). In a contaminated surface experiment, MNV with 5% FBS was inoculated on rayon sheets, and the result showed FdCa(OH) solution could markedly reduce virus titer within 1min. When mouse feces were spiked with MNV and FdCa(OH) powder as 10% and 20% w/w was added to the feces, these concentrations could inactivate the virus within 30min and 15min, respectively. Whereas, FdCa(OH) suspension at 2.5% and 5% could inactivate the virus within 30min and at 1% within 45min. These and additional results obtained here indicate that FdCa(OH) is an effective virucidal agent against MNV, and can serve as a useful alternative disinfectant for inactivation and prevention of human norovirus in house and hospital.
[Mh] Termos MeSH primário: Hidróxido de Cálcio/farmacologia
Desinfetantes/farmacologia
Aditivos Alimentares/farmacologia
Viabilidade Microbiana/efeitos dos fármacos
Norovirus/efeitos dos fármacos
Norovirus/fisiologia
Inativação de Vírus
[Mh] Termos MeSH secundário: Animais
Fezes/virologia
Camundongos
Fatores de Tempo
Carga Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disinfectants); 0 (Food Additives); PF5DZW74VN (Calcium Hydroxide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171022
[St] Status:MEDLINE


  7 / 3275 MEDLINE  
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[PMID]:29284004
[Au] Autor:Fu JG; Shi C; Xu C; Lin Q; Zhang J; Yi QH; Zhang J; Bao CJ; Huo X; Zhu YF; Ai J; Xing Z
[Ad] Endereço:Medical School and Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing, China.
[Ti] Título:Outbreaks of acute gastroenteritis associated with a re-emerging GII.P16-GII.2 norovirus in the spring of 2017 in Jiangsu, China.
[So] Source:PLoS One;12(12):e0186090, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A total of 64 acute gastroenteritis outbreaks with 2,953 patients starting in December of 2016 and occurring mostly in the late spring of 2017 were reported in Jiangsu, China. A recombinant GII.P16-GII.2 norovirus variant was associated with 47 outbreaks (73.4%) for the gastroenteritis epidemic, predominantly occurring in February and March of 2017. Sequence analysis of the RNA-dependent RNA polymerase (RdRp) and capsid protein of the viral isolates from these outbreaks confirmed that this GII.P16-GII.2 strain was the GII.P16-GII.2 variant with the intergenotypic recombination, identified in Taiwan, Hong Kong, and other cities in China in 2016. This GII.P16-GII.2 recombinant variant appeared to a re-emerging strain, firstly identified in 2011-2012 from Japan and USA but might be independently originated from other GII.P16-GII.2 variants for sporadic and outbreaks of gastroenteritis in Japan and China before 2016. Further identification of unique amino acid mutations in both VP1 and RdRp of NoV strain as shown in this report may provide insight in explaining its structural and antigenic changes, potentially critical for the variant recombinant to gain its predominance in causing regional and worldwide epidemics.
[Mh] Termos MeSH primário: Infecções por Caliciviridae/epidemiologia
Surtos de Doenças
Gastroenterite/epidemiologia
Norovirus/isolamento & purificação
[Mh] Termos MeSH secundário: Doença Aguda
China/epidemiologia
Genes Virais
Seres Humanos
Norovirus/classificação
Norovirus/genética
Norovirus/patogenicidade
Filogenia
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171229
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186090


  8 / 3275 MEDLINE  
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[PMID]:29311445
[Au] Autor:Somura Y; Kimoto K; Oda M; Okutsu Y; Kato R; Suzuki Y; Siki D; Hirai A; Akiba T; Shinkai T; Sadamasu K
[Ad] Endereço:Tokyo Metropolitan Institute of Public Health.
[Ti] Título:[Serial Food Poisoning Outbreaks Caused by Norovirus-Contaminated Shredded Dried Laver Seaweed Provided at School Lunch, Tokyo, 2017].
[So] Source:Shokuhin Eiseigaku Zasshi;58(6):260-267, 2017.
[Is] ISSN:1882-1006
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:In February 2017, four food poisoning outbreaks occurred in Tokyo, involving ten schools. Shredded dried laver seaweed processed by a single food manufacturer in December 2016 was provided in common for the school meals that caused all four outbreaks. Of 4,209 persons exposed, 1,193 (28.3%) had symptoms of gastroenteritis. Norovirus (NoV) GII was detected in 207 (78.1%) of 265 cases by real-time RT-PCR. Thirty-one shredded dried laver seaweed samples were examined and seven (22.6%) of them were positive for NoV GII. PCR fragments of NoV ORF1/2 junction region (302 bp) from seven shredded dried laver seaweed samples and 20 clinical samples derived from the four outbreaks were sequenced. All of them displayed complete homology, and the genotype was classified as GII.17. A nearly full-length sequence (7,420 bp) of NoV RNA derived from a case was obtained by next-generation sequencer analysis and phylogenetic analysis indicated that this strain belongs to the same cluster as Hu/GII/JP/2015/GII.P17_GII.17/Kawasaki308. Thus, our investigation elucidated that the causative agent of these four serial food poisoning outbreaks was NoV GII.17 and the infectious source was a single batch of shredded dried laver seaweed. The water activity of the shredded dried laver seaweed was found to be 0.119 to 0.129. It was epidemiologically clarified that NoV does not lose infectivity for about two months even in the dry state. We conclude that a large diffuse outbreak of food poisoning caused by NoV GII.17 contamination of shredded dried laver seaweed had occurred in Tokyo. Our elucidation of the causative agent indicated that the food poisoning outbreaks in multiple areas of Japan, including Tokyo, during January to February 2017 were caused by the same contaminated food.
[Mh] Termos MeSH primário: Infecções por Caliciviridae/etiologia
Infecções por Caliciviridae/virologia
Surtos de Doenças/estatística & dados numéricos
Análise de Alimentos
Contaminação de Alimentos/análise
Doenças Transmitidas por Alimentos/etiologia
Doenças Transmitidas por Alimentos/virologia
Almoço
Norovirus/isolamento & purificação
Instituições Acadêmicas/estatística & dados numéricos
Alga Marinha/virologia
[Mh] Termos MeSH secundário: Infecções por Caliciviridae/epidemiologia
Cobicistat
Combinação de Medicamentos
Emtricitabina
Doenças Transmitidas por Alimentos/epidemiologia
Norovirus/classificação
Norovirus/genética
Quinolonas
RNA Viral/isolamento & purificação
Tenofovir/análogos & derivados
Fatores de Tempo
Tóquio/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drug Combinations); 0 (Quinolones); 0 (RNA, Viral); 0 (genovoya); 99YXE507IL (Tenofovir); G70B4ETF4S (Emtricitabine); LW2E03M5PG (Cobicistat)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180123
[Lr] Data última revisão:
180123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.3358/shokueishi.58.260


  9 / 3275 MEDLINE  
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[PMID]:28941906
[Au] Autor:Yang Z; Chambers H; DiCaprio E; Gao G; Li J
[Ad] Endereço:Department of Biology, College of Life Science, Huzhou University, Huzhou, Zhejiang, China.
[Ti] Título:Internalization and dissemination of human norovirus and Tulane virus in fresh produce is plant dependent.
[So] Source:Food Microbiol;69:25-32, 2018 Feb.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Human norovirus (NoV) is a leading cause of fresh produce associated outbreaks. Previous research indicates that the roots of growing leafy greens and berries internalize human NoV. However the effect of plant type and inoculum level on internalization rates has not been directly compared. In this study we compared the internalization and dissemination rates of human NoV and its surrogate, Tulane virus (TV) in green onion, radishes, and Romaine lettuce. We also evaluated the effect inoculum level and plant growth matrix on the rate of viral internalization. In the hydroponic growth system, we detected internalization and dissemination of human NoV RNA in green onions. In hydroponically growing green onions inoculated with high titer TV, we found higher rates of internalization and dissemination compared to green onions inoculated with low titer TV. In soil growth systems, no infectious TV was detected in either green onion or radishes. However, in Romaine lettuce plants grown in soil approximately 4 log PFU/g was recovered from all tissues on day 14 p.i. Overall, we found that the type of plant, growth matrix, and the inoculum level influences the internalization and dissemination of human NoV and TV.
[Mh] Termos MeSH primário: Caliciviridae/fisiologia
Contaminação de Alimentos/análise
Alface/virologia
Norovirus/fisiologia
Cebolas/virologia
Raphanus/virologia
Verduras/virologia
Internalização do Vírus
[Mh] Termos MeSH secundário: Caliciviridae/genética
Caliciviridae/isolamento & purificação
Seres Humanos
Alface/crescimento & desenvolvimento
Norovirus/genética
Norovirus/isolamento & purificação
Cebolas/crescimento & desenvolvimento
Raphanus/crescimento & desenvolvimento
Microbiologia do Solo
Verduras/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170925
[St] Status:MEDLINE


  10 / 3275 MEDLINE  
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Leite, José Paulo Gagliardi
Miagostovich, Marize Pereira
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[PMID]:29236779
[Au] Autor:Barreira DMPG; Fumian TM; Tonini MAL; Volpini LPB; Santos RP; Ribeiro ALC; Leite JPG; Souza MTBME; Brasil P; da Cunha DC; Miagostovich MP; Spano LC
[Ad] Endereço:Laboratory of Virology and Infectious Gastroenteritis, Pathology Department, Health Science Center, Federal University of Espírito Santo, Av. Marechal Campos 1468, Maruípe, Vitória, ES, Brazil.
[Ti] Título:Detection and molecular characterization of the novel recombinant norovirus GII.P16-GII.4 Sydney in southeastern Brazil in 2016.
[So] Source:PLoS One;12(12):e0189504, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Noroviruses are the leading cause of acute gastroenteritis (AGE) in all age groups worldwide. Despite the high genetic diversity of noroviruses, most AGE outbreaks are caused by a single norovirus genotype: GII.4. Since 1995, several different variants of norovirus GII.4 have been associated with pandemics, with each variant circulating for 3 to 8 years. The Sydney_2012 variant was first reported in Australia and then in other countries. A new variant, GII.P16-GII.4, was recently described in Japan and South Korea and then in the USA, France, Germany and England. In our study, 190 faecal specimens were collected from children admitted to a paediatric hospital and a public health facility during a surveillance study of sporadic cases of AGE conducted between January 2015 and July 2016. The norovirus was detected by RT-qPCR in 51 samples (26.8%), and in 37 of them (72.5%), the ORF1-2 junction was successfully sequenced. The new recombinant GII.P16-GII.4 Sydney was revealed for the first time in Brazil in 2016 and predominated among other strains (9 GII.Pe-GII.4, 3 GII.P17-GII.17, 1 GII.Pg-GII.1, 1 GII.P16-GII.3 and 1 GII.PNA-GII.4). The epidemiological significance of this new recombinant is still unknown, but continuous surveillance studies may evaluate its impact on the population, its potential to replace the first recombinant GII.Pe-GII.4 Sydney 2012 variant, and the emergence of new recombinant forms of GII.P16.
[Mh] Termos MeSH primário: Norovirus/genética
Recombinação Genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Brasil/epidemiologia
Infecções por Caliciviridae/epidemiologia
Criança
Pré-Escolar
Seres Humanos
Lactente
Norovirus/classificação
Filogenia
Homologia de Sequência de Aminoácidos
Proteínas Virais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189504



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