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[PMID]:28986291
[Au] Autor:Meli ML; Berger A; Willi B; Spiri AM; Riond B; Hofmann-Lehmann R
[Ad] Endereço:Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland; Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland. Electronic address: mmeli@vetclinics.uzh.ch.
[Ti] Título:Molecular detection of feline calicivirus in clinical samples: A study comparing its detection by RT-qPCR directly from swabs and after virus isolation.
[So] Source:J Virol Methods;251:54-60, 2018 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Feline caliciviruses (FCVs) are non-enveloped RNA viruses that exhibit high genetic variation. Two reverse transcription quantitative polymerase chain reaction (RT-qPCR) FCV assays (S1 and S2) were evaluated using samples from 300 field cats. The direct detection of FCV in swabs and after propagation in cell culture, as well as the influence of storage conditions, was assessed. FCV-RNA detectability on dry swabs was similar after storage at either 4°C or -20°C, but viral burdens were maintained for a longer time period when viral transport medium was used. A total of 97 (32%) samples was considered FCV PCR-positive. Of these, 81% and 77% tested positive directly from swabs using S1 and S2, respectively; 84% and 81% tested positive after enrichment in cell culture, respectively. Combined detection by RT-PCR directly from swabs and after VI was most sensitive (up to 96%). Neither of the methods alone were able to detect all FCV-positive samples. In conclusion, clinical samples should be collected in viral transport medium, stored at ≤4°C and processed as soon as possible. The combination of cell culture with RT-qPCR or detection directly from swabs using a combination of different RT-qPCR assays is recommended to reach a high sensitivity of FCV detection.
[Mh] Termos MeSH primário: Infecções por Caliciviridae/veterinária
Calicivirus Felino/isolamento & purificação
Doenças do Gato/diagnóstico
Doenças do Gato/virologia
Técnicas de Diagnóstico Molecular/métodos
Reação em Cadeia da Polimerase em Tempo Real/métodos
Manejo de Espécimes/métodos
[Mh] Termos MeSH secundário: Animais
Infecções por Caliciviridae/diagnóstico
Infecções por Caliciviridae/virologia
Gatos
Sensibilidade e Especificidade
Cultura de Vírus
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171008
[St] Status:MEDLINE


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[PMID]:28691897
[Au] Autor:Sato H; Sehata G; Okada N; Iwamoto K; Masubuchi K; Kainuma R; Noda T; Igarashi T; Sawada T; Noro T; Oishi E
[Ad] Endereço:1​Kyoto Biken Laboratories, Inc., 24-16 Makishima-cho, Uji-shi, Kyoto 611-0041, Japan.
[Ti] Título:Intranasal immunization with inactivated feline calicivirus particles confers robust protection against homologous virus and suppression against heterologous virus in cats.
[So] Source:J Gen Virol;98(7):1730-1738, 2017 Jul.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The protective efficacy of intranasal (IN) administration of inactivated feline calicivirus (FCV) vaccine against homologous or heterologous FCV infection was investigated. Groups of cats immunized with the experimental inactivated, non-adjuvanted FCV vaccine via either the IN or subcutaneous (SC) route were exposed to homologous or highly heterologous FCV. Both the IN and SC immunization protocols established robust protection against homologous FCV infection. Although neither immunization regimen conferred protection against the heterologous strain, clinical scores and virus titres of oral swabs were lower in cats in the IN group compared to those in the SC group, accompanying a faster neutralizing antibody response against the heterologous virus in cats in the IN group. The IN group secreted more IgA specific to FCV proteins in oral washes (lavage fluids from the oral cavity) than the SC group. IN immunization with an inactivated whole FCV particle, which protects cats from homologous virus exposure and shortens the period of heterologous virus shedding, may serve as a better platform for anti-FCV vaccine.
[Mh] Termos MeSH primário: Anticorpos Neutralizantes/imunologia
Anticorpos Antivirais/imunologia
Calicivirus Felino/imunologia
Vacinação/veterinária
Vacinas de Produtos Inativados/imunologia
Vacinas Virais/imunologia
[Mh] Termos MeSH secundário: Administração Intranasal
Animais
Infecções por Caliciviridae/imunologia
Infecções por Caliciviridae/prevenção & controle
Doenças do Gato/imunologia
Doenças do Gato/prevenção & controle
Doenças do Gato/virologia
Gatos
Imunoglobulina A/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Neutralizing); 0 (Antibodies, Viral); 0 (Immunoglobulin A); 0 (Vaccines, Inactivated); 0 (Viral Vaccines)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170804
[Lr] Data última revisão:
170804
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000827


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[PMID]:28472060
[Au] Autor:Goda H; Yamaoka H; Nakayama-Imaohji H; Kawata H; Horiuchi I; Fujita Y; Nagao T; Tada A; Terada A; Kuwahara T
[Ad] Endereço:Honbu Sankei Co. Ltd., 2-2-53 Shiromi, Chuou-ku, Osaka, Japan.
[Ti] Título:Microbicidal effects of weakly acidified chlorous acid water against feline calicivirus and Clostridium difficile spores under protein-rich conditions.
[So] Source:PLoS One;12(5):e0176718, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sanitation of environmental surfaces with chlorine based-disinfectants is a principal measure to control outbreaks of norovirus or Clostridium difficile. The microbicidal activity of chlorine-based disinfectants depends on the free available chlorine (FAC), but their oxidative potential is rapidly eliminated by organic matter. In this study, the microbicidal activities of weakly acidified chlorous acid water (WACAW) and sodium hypochlorite solution (NaClO) against feline calcivirus (FCV) and C. difficile spores were compared in protein-rich conditions. WACAW inactivated FCV and C. difficile spores better than NaClO under all experimental conditions used in this study. WACAW above 100 ppm FAC decreased FCV >4 log10 within 30 sec in the presence of 0.5% each of bovine serum albumin (BSA), polypeptone or meat extract. Even in the presence of 5% BSA, WACAW at 600 ppm FAC reduced FCV >4 log10 within 30 sec. Polypeptone inhibited the virucidal activity of WACAW against FCV more so than BSA or meat extract. WACAW at 200 ppm FAC decreased C. difficile spores >3 log10 within 1 min in the presence of 0.5% polypeptone. The microbicidal activity of NaClO was extensively diminished in the presence of organic matter. WACAW recovered its FAC to the initial level after partial neutralization by sodium thiosulfate, while no restoration of the FAC was observed in NaClO. These results indicate that WACAW is relatively stable under organic matter-rich conditions and therefore may be useful for treating environmental surfaces contaminated by human excretions.
[Mh] Termos MeSH primário: Calicivirus Felino/efeitos dos fármacos
Cloretos/farmacologia
Clostridium difficile/efeitos dos fármacos
Esporos Bacterianos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Gatos
Clostridium difficile/crescimento & desenvolvimento
Seres Humanos
Ratos
Soroalbumina Bovina/metabolismo
Espectrofotometria Ultravioleta
Tiossulfatos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chlorides); 0 (Thiosulfates); 27432CM55Q (Serum Albumin, Bovine); 7JRT833T5M (chlorous acid); HX1032V43M (sodium thiosulfate)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0176718


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[PMID]:28235708
[Au] Autor:Thomas S; Lappin DF; Spears J; Bennett D; Nile C; Riggio MP
[Ad] Endereço:Nestlé Purina Research, St. Louis, MO, USA.
[Ti] Título:Prevalence of feline calicivirus in cats with odontoclastic resorptive lesions and chronic gingivostomatitis.
[So] Source:Res Vet Sci;111:124-126, 2017 Apr.
[Is] ISSN:1532-2661
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Feline odontoclastic resorptive lesion (FORL) and feline chronic gingivostomatitis (FCGS) are two of the most common diseases of the feline oral cavity. While evidence is emerging that FCGS is caused by gingival inflammation initiated and perpetuated by the oral microbiota, little is known in this regard for FORL. Feline calicivirus (FCV) has been associated with the presence of FCGS and is thought to play a role in the initiation of this disease. In this study, the incidence of FCV was investigated in cats with FORL and FCGS, and compared to unaffected controls. FCV was detected by viral culture. The incidence of FCV was as follows: 6 (24.0%) of 24 control cats, 9 (22.5%) of 40 cats with FORL and 15 (60.0%) of 25 cats with FCGS were positive for FCV. There was a significant difference in FCV incidence between all the groups (p=0.003) but none between the control group and the FORL group. However, significant differences were observed in the incidence of FCV between control and FCGS (p=0.010) and between FORL and FCGS (p=0.006). It is concluded that although FCV may be associated with FCGS, it appears unlikely to play a role in FORL.
[Mh] Termos MeSH primário: Infecções por Caliciviridae/veterinária
Calicivirus Felino/isolamento & purificação
Doenças do Gato/epidemiologia
Reabsorção da Raiz/veterinária
Estomatite Herpética/veterinária
[Mh] Termos MeSH secundário: Animais
Infecções por Caliciviridae/epidemiologia
Infecções por Caliciviridae/virologia
Estudos de Casos e Controles
Doenças do Gato/virologia
Gatos
Feminino
Incidência
Masculino
Missouri/epidemiologia
Prevalência
Reabsorção da Raiz/epidemiologia
Reabsorção da Raiz/virologia
Estomatite Herpética/epidemiologia
Estomatite Herpética/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170226
[St] Status:MEDLINE


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[PMID]:28190489
[Au] Autor:Sun Y; Deng M; Peng Z; Hu R; Chen H; Wu B
[Ad] Endereço:Department of Preventive Veterinary Medicine, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
[Ti] Título:Genetic and phylogenetic analysis of feline calicivirus isolates in China.
[So] Source:Vet J;220:24-27, 2017 Feb.
[Is] ISSN:1532-2971
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to determine the genetic diversity of Chinese feline calicivirus (FCV) isolates and their phylogenetic relationship with isolates from elsewhere in the world. Phylogenetic analysis was performed based on the partial open reading frame (ORF) 2 sequences (regions B-F) of 21 Chinese FCV isolates and 30 global isolates. The Chinese isolates included 13 isolates from Wuhan, which were isolated in this study, and eight previously published isolates. Sixteen Chinese isolates and two Japanese isolates formed a distinct phylogenetic cluster. Phylogenetic analysis based on the sequences of the complete genome, ORF1, ORF2 and ORF3 of selected isolates supported the above findings. Genogroup analysis revealed that FCV genogroup II is present in China. These findings suggest that Chinese FCV isolates are closely related to Japanese FCV isolates.
[Mh] Termos MeSH primário: Infecções por Caliciviridae/veterinária
Calicivirus Felino/genética
Proteínas do Capsídeo/genética
Doenças do Gato/virologia
Variação Genética
[Mh] Termos MeSH secundário: Animais
Infecções por Caliciviridae/virologia
Gatos
China
Filogenia
Análise de Sequência de RNA/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsid Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170627
[Lr] Data última revisão:
170627
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170214
[St] Status:MEDLINE


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[PMID]:28045956
[Au] Autor:Wang J; Liu L; Wang J; Sun X; Yuan W
[Ad] Endereço:Inspection and Quarantine Technical Center of Hebei Entry-Exit Inspection and Quarantine Bureau, Xinhua District, Shijiazhuang, Hebei, China.
[Ti] Título:Recombinase Polymerase Amplification Assay-A Simple, Fast and Cost-Effective Alternative to Real Time PCR for Specific Detection of Feline Herpesvirus-1.
[So] Source:PLoS One;12(1):e0166903, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Feline herpesvirus 1 (FHV-1), an enveloped dsDNA virus, is one of the major pathogens of feline upper respiratory tract disease (URTD) and ocular disease. Currently, polymerase chain reaction (PCR) remains the gold standard diagnostic tool for FHV-1 infection but is relatively expensive, requires well-equipped laboratories and is not suitable for field tests. Recombinase polymerase amplification (RPA), an isothermal gene amplification technology, has been explored for the molecular diagnosis of infectious diseases. In this study, an exo-RPA assay for FHV-1 detection was developed and validated. Primers targeting specifically the thymidine kinase (TK) gene of FHV-1 were designed. The RPA reaction was performed successfully at 39°C and the results were obtained within 20 min. Using different copy numbers of recombinant plasmid DNA that contains the TK gene as template, we showed the detection limit of exo-RPA was 102 copies DNA/reaction, the same as that of real time PCR. The exo-RPA assay did not cross-detect feline panleukopenia virus, feline calicivirus, bovine herpesvirus-1, pseudorabies virus or chlamydia psittaci, a panel of pathogens important in feline URTD or other viruses in Alphaherpesvirinae, demonstrating high specificity. The assay was validated by testing 120 nasal and ocular conjunctival swabs of cats, and the results were compared with those obtained with real-time PCR. Both assays provided the same testing results in the clinical samples. Compared with real time PCR, the exo-RPA assay uses less-complex equipment that is portable and the reaction is completed much faster. Additionally, commercial RPA reagents in vacuum-sealed pouches can tolerate temperatures up to room temperature for days without loss of activity, suitable for shipment and storage for field tests. Taken together, the exo-RPA assay is a simple, fast and cost-effective alternative to real time PCR, suitable for use in less advanced laboratories and for field detection of FHV-1 infection.
[Mh] Termos MeSH primário: Calicivirus Felino/isolamento & purificação
Doenças do Gato/virologia
Reação em Cadeia da Polimerase em Tempo Real/economia
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Recombinases/metabolismo
[Mh] Termos MeSH secundário: Animais
Calicivirus Felino/genética
Gatos
Análise Custo-Benefício
Plasmídeos/genética
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Temperatura Ambiente
Timidina Quinase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Recombinases); EC 2.7.1.21 (Thymidine Kinase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170104
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0166903


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[PMID]:28040848
[Au] Autor:Tomb RM; Maclean M; Coia JE; Graham E; McDonald M; Atreya CD; MacGregor SJ; Anderson JG
[Ad] Endereço:The Robertson Trust Laboratory for Electronic Sterilisation Technologies (ROLEST), Department of Electronic & Electrical Engineering, University of Strathclyde, Royal College Building, 204 George Street, Glasgow, G1 1XW, Scotland, UK. rachael.tomb.2013@uni.strath.ac.uk.
[Ti] Título:New Proof-of-Concept in Viral Inactivation: Virucidal Efficacy of 405 nm Light Against Feline Calicivirus as a Model for Norovirus Decontamination.
[So] Source:Food Environ Virol;9(2):159-167, 2017 Jun.
[Is] ISSN:1867-0342
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The requirement for novel decontamination technologies for use in hospitals is ever present. One such system uses 405 nm visible light to inactivate microorganisms via ROS-generated oxidative damage. Although effective for bacterial and fungal inactivation, little is known about the virucidal effects of 405 nm light. Norovirus (NoV) gastroenteritis outbreaks often occur in the clinical setting, and this study was designed to investigate potential inactivation effects of 405 nm light on the NoV surrogate, feline calicivirus (FCV). FCV was exposed to 405 nm light whilst suspended in minimal and organically-rich media to establish the virucidal efficacy and the effect biologically-relevant material may play in viral susceptibility. Antiviral activity was successfully demonstrated with a 4 Log (99.99%) reduction in infectivity when suspended in minimal media evident after a dose of 2.8 kJ cm . FCV exposed in artificial faeces, artificial saliva, blood plasma and other organically rich media exhibited an equivalent level of inactivation using between 50-85% less dose of the light, indicating enhanced inactivation when the virus is present in organically-rich biologically-relevant media. Further research in this area could aid in the development of 405 nm light technology for effective NoV decontamination within the hospital environment.
[Mh] Termos MeSH primário: Calicivirus Felino/efeitos da radiação
Descontaminação/métodos
Desinfetantes/farmacologia
Norovirus/efeitos da radiação
Inativação de Vírus/efeitos da radiação
[Mh] Termos MeSH secundário: Animais
Infecções por Caliciviridae/prevenção & controle
Infecções por Caliciviridae/virologia
Calicivirus Felino/fisiologia
Gatos
Linhagem Celular
Descontaminação/instrumentação
Seres Humanos
Luz
Modelos Biológicos
Norovirus/fisiologia
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disinfectants)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170810
[Lr] Data última revisão:
170810
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170102
[St] Status:MEDLINE
[do] DOI:10.1007/s12560-016-9275-z


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[PMID]:28040178
[Au] Autor:Joshi S; Howell AB; D'Souza DH
[Ad] Endereço:The University of Tennessee-Knoxville, Department of Food Science and Technology, 2600 River Drive, Knoxville, TN 37966, USA.
[Ti] Título:Blueberry proanthocyanidins against human norovirus surrogates in model foods and under simulated gastric conditions.
[So] Source:Food Microbiol;63:263-267, 2017 May.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Blueberry proanthocyanidins (B-PAC) are known to decrease titers of human norovirus surrogates in vitro. The application of B-PAC as therapeutic or preventive options against foodborne viral illness needs to be determined using model foods and simulated gastric conditions in vitro. The objective of this study was to evaluate the antiviral effect of B-PAC in model foods (apple juice (AJ) and 2% reduced fat milk) and simulated gastrointestinal fluids against cultivable human norovirus surrogates (feline calicivirus; FCV-F9 and murine norovirus; MNV-1) over 24 h at 37 °C. Equal amounts of each virus (5 log PFU/ml) was mixed with B-PAC (1, 2 and 5 mg/ml) prepared either in AJ, or 2% milk, or simulated gastric fluids and incubated over 24 h at 37 °C. Controls included phosphate buffered saline, malic acid (pH 7.2), AJ, 2% milk or simulated gastric and intestinal fluids incubated with virus over 24 h at 37 °C. The tested viruses were reduced to undetectable levels within 15 min with B-PAC (1, 2 and 5 mg/ml) in AJ (pH 3.6). However, antiviral activity of B-PAC was reduced in milk. FCV-F9 was reduced by 0.4 and 1.09 log PFU/ml with 2 and 5 mg/ml B-PAC in milk, respectively and MNV-1 titers were reduced by 0.81 log PFU/ml with 5 mg/ml B-PAC in milk after 24 h. B-PAC at 5 mg/ml in simulated intestinal fluid reduced titers of the tested viruses to undetectable levels within 30 min. Overall, these results show the potential of B-PAC as preventive and therapeutic options for foodborne viral illnesses.
[Mh] Termos MeSH primário: Mirtilos Azuis (Planta)/química
Calicivirus Felino/crescimento & desenvolvimento
Norovirus/crescimento & desenvolvimento
Proantocianidinas/farmacologia
[Mh] Termos MeSH secundário: Animais
Antivirais/farmacologia
Calicivirus Felino/efeitos dos fármacos
Microbiologia de Alimentos
Doenças Transmitidas por Alimentos/prevenção & controle
Sucos de Frutas e Vegetais/análise
Sucos de Frutas e Vegetais/virologia
Ácido Gástrico/química
Trato Gastrointestinal/virologia
Seres Humanos
Concentração de Íons de Hidrogênio
Leite/virologia
Norovirus/classificação
Norovirus/efeitos dos fármacos
Ensaio de Placa Viral
Inativação de Vírus
Vírus/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Proanthocyanidins)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170307
[Lr] Data última revisão:
170307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170102
[St] Status:MEDLINE


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[PMID]:27865960
[Au] Autor:Tian J; Hu X; Liu D; Wu H; Qu L
[Ad] Endereço:Division of Zoonosis of Natural Foci, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, PR China. Electronic address: tj6049345@126.com.
[Ti] Título:Identification of Inonotus obliquus polysaccharide with broad-spectrum antiviral activity against multi-feline viruses.
[So] Source:Int J Biol Macromol;95:160-167, 2017 Feb.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Inonotus obliquus polysaccharides (IOPs) are a potential drug for the prevention and treatment of cancer, cardiopathy, diabetes, AIDs, pancreatitis and other diseases. In this study, we found that IOP can act as a broad-spectrum antiviral drug against feline viruses in the in vitro experiment. Using cell models of feline calicivirus (FCV), we demonstrated that IOP treatment was capable of exhibiting anti-FCV strain F9 activity in cell-based assays and also showed low cytotoxicity. Investigation of the mechanism of action of the compound revealed that IOP treatment induces its inhibitory actions directly on virus particles through blocking viral binding/absorpting. The inhibitory activity against other FCV isolates from China was also identified. More importantly, we found that IOP exhibited broad-spectrum antiviral activity against the feline herpesvirus 1, feline influenza virus H3N2 and H5N6, feline panleukopenia virus and feline infectious peritonitis virus that can contribute to respiratory and gastrointestinal diseases in cats. These findings suggest that IOP may be a potential broad-spectrum antiviral drug against feline viruses.
[Mh] Termos MeSH primário: Antivirais/farmacologia
Basidiomycota/química
Calicivirus Felino/efeitos dos fármacos
Polissacarídeos Fúngicos/farmacologia
[Mh] Termos MeSH secundário: Animais
Antivirais/química
Antivirais/isolamento & purificação
Calicivirus Felino/fisiologia
Gatos
Relação Dose-Resposta a Droga
Polissacarídeos Fúngicos/química
Polissacarídeos Fúngicos/isolamento & purificação
Internalização do Vírus/efeitos dos fármacos
Replicação Viral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Fungal Polysaccharides)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170403
[Lr] Data última revisão:
170403
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161121
[St] Status:MEDLINE


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[PMID]:27807684
[Au] Autor:Seo DJ; Choi C
[Ad] Endereço:Department of Food and Nutrition, College of Biotechnology and Natural Resources, Chung-Ang University, Anseong, Gyeonggi, 17546, South Korea.
[Ti] Título:Inhibition of Murine Norovirus and Feline Calicivirus by Edible Herbal Extracts.
[So] Source:Food Environ Virol;9(1):35-44, 2017 Mar.
[Is] ISSN:1867-0342
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Human noroviruses (HuNoVs) cause foodborne and waterborne viral gastroenteritis worldwide. Because HuNoV culture systems have not been developed thus far, no available medicines or vaccines preventing infection with HuNoVs exist. Some herbal extracts were considered as phytomedicines because of their bioactive components. In this study, the inhibitory effects of 29 edible herbal extracts against the norovirus surrogates murine norovirus (MNV) and feline calicivirus (FCV) were examined. FCV was significantly inhibited to 86.89 ± 2.01 and 48.71 ± 7.38% by 100 µg/mL of Camellia sinensis and Ficus carica, respectively. Similarly, ribavirin at a concentration of 100 µM significantly reduced the titer of FCV by 77.69 ± 10.40%. Pleuropterus multiflorus (20 µg/mL) showed antiviral activity of 53.33 ± 5.77, and 50.00 ± 16.67% inhibition was observed after treatment with 20 µg/mL of Alnus japonica. MNV was inhibited with ribavirin by 59.22 ± 16.28% at a concentration of 100 µM. Interestingly, MNV was significantly inhibited with 150 µg/mL Inonotus obliquus and 50 µg/mL Crataegus pinnatifida by 91.67 ± 5.05 and 57.66 ± 3.36%, respectively. Treatment with 20 µg/mL Coriandrum sativum slightly reduced MNV by 45.24 ± 4.12%. The seven herbal extracts of C. sinensis, F. carica, P. multiflorus, A. japonica, I. obliquus, C. pinnatifida, and C. sativum may have the potential to control noroviruses without cytotoxicity.
[Mh] Termos MeSH primário: Antivirais/farmacologia
Calicivirus Felino/efeitos dos fármacos
Norovirus/efeitos dos fármacos
Extratos Vegetais/farmacologia
Plantas/química
[Mh] Termos MeSH secundário: Animais
Antivirais/isolamento & purificação
Calicivirus Felino/crescimento & desenvolvimento
Norovirus/crescimento & desenvolvimento
Extratos Vegetais/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Plant Extracts)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161104
[St] Status:MEDLINE
[do] DOI:10.1007/s12560-016-9269-x



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