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  1 / 2518 MEDLINE  
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[PMID]:29382836
[Au] Autor:Füzik T; Formanová P; Ruzek D; Yoshii K; Niedrig M; Plevka P
[Ad] Endereço:Structural Virology, Central European Institute of Technology, Masaryk University, Kamenice 753/5, 62500, Brno, Czech Republic.
[Ti] Título:Structure of tick-borne encephalitis virus and its neutralization by a monoclonal antibody.
[So] Source:Nat Commun;9(1):436, 2018 01 30.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tick-borne encephalitis virus (TBEV) causes 13,000 cases of human meningitis and encephalitis annually. However, the structure of the TBEV virion and its interactions with antibodies are unknown. Here, we present cryo-EM structures of the native TBEV virion and its complex with Fab fragments of neutralizing antibody 19/1786. Flavivirus genome delivery depends on membrane fusion that is triggered at low pH. The virion structure indicates that the repulsive interactions of histidine side chains, which become protonated at low pH, may contribute to the disruption of heterotetramers of the TBEV envelope and membrane proteins and induce detachment of the envelope protein ectodomains from the virus membrane. The Fab fragments bind to 120 out of the 180 envelope glycoproteins of the TBEV virion. Unlike most of the previously studied flavivirus-neutralizing antibodies, the Fab fragments do not lock the E-proteins in the native-like arrangement, but interfere with the process of virus-induced membrane fusion.
[Mh] Termos MeSH primário: Anticorpos Neutralizantes/química
Anticorpos Antivirais/química
Vírus da Encefalite Transmitidos por Carrapatos/ultraestrutura
Fragmentos Fab das Imunoglobulinas/química
Proteínas Virais/química
Vírion/ultraestrutura
[Mh] Termos MeSH secundário: Anticorpos Neutralizantes/biossíntese
Anticorpos Antivirais/biossíntese
Linhagem Celular Tumoral
Microscopia Crioeletrônica
Vírus da Encefalite Transmitidos por Carrapatos/genética
Vírus da Encefalite Transmitidos por Carrapatos/metabolismo
Expressão Gênica
Seres Humanos
Concentração de Íons de Hidrogênio
Fragmentos Fab das Imunoglobulinas/biossíntese
Fusão de Membrana/genética
Neurônios/patologia
Neurônios/virologia
Domínios Proteicos
Multimerização Proteica
Proteínas Virais/genética
Proteínas Virais/metabolismo
Vírion/genética
Vírion/metabolismo
Internalização do Vírus
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Neutralizing); 0 (Antibodies, Viral); 0 (Immunoglobulin Fab Fragments); 0 (Viral Proteins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180201
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-018-02882-0


  2 / 2518 MEDLINE  
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[PMID]:28453842
[Au] Autor:Lipowski D; Popiel M; Perlejewski K; Nakamura S; Bukowska-Osko I; Rzadkiewicz E; Dzieciatkowski T; Milecka A; Wenski W; Ciszek M; Debska-Slizien A; Ignacak E; Cortes KC; Pawelczyk A; Horban A; Radkowski M; Laskus T
[Ad] Endereço:Department of Infectious Diseases, Warsaw Medical University, Warsaw, Poland.
[Ti] Título:A Cluster of Fatal Tick-borne Encephalitis Virus Infection in Organ Transplant Setting.
[So] Source:J Infect Dis;215(6):896-901, 2017 03 15.
[Is] ISSN:1537-6613
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Background: Tick-borne encephalitis virus (TBEV) infection has become a major health problem in Europe and is currently a common cause of viral brain infection in many countries. Encephalitis in transplant recipients, althrough rare, is becoming a recognized complication. Our study provides the first description of transmission of TBEV through transplantation of solid organs. Methods: Three patients who received solid organ transplants from a single donor (2 received kidney, and 1 received liver) developed encephalitis 17-49 days after transplantation and subsequently died. Blood and autopsy tissue samples were tested by next-generation sequencing (NGS) and reverse transcription polymerase chain reaction (RT-PCR). Results: All 3 recipients were first analyzed in autopsy brain tissue samples and/or cerebrospinal fluid by NGS, which yielded 24-52 million sequences per sample and 9-988 matched TBEV sequences in each patient. The presence of TBEV was confirmed by RT-PCR in all recipients and in the donor, and direct sequencing of amplification products corroborated the presence of the same viral strain. Conclusions: We demonstrated transmission of TBEV by transplantation of solid organs. In such a setting, TBEV infection may be fatal, probably due to pharmacological immunosuppression. Organ donors should be screened for TBEV when coming from or visiting endemic areas.
[Mh] Termos MeSH primário: Encéfalo/virologia
Vírus da Encefalite Transmitidos por Carrapatos/isolamento & purificação
Encefalite Transmitida por Carrapatos/transmissão
Transplante de Órgãos/efeitos adversos
Doadores de Tecidos
[Mh] Termos MeSH secundário: Adulto
Autopsia
Seleção do Doador
Encefalite Transmitida por Carrapatos/etiologia
Evolução Fatal
Seres Humanos
Masculino
Meia-Idade
Polônia
Complicações Pós-Operatórias/etiologia
RNA Viral/sangue
Análise de Sequência de RNA
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1093/infdis/jix040


  3 / 2518 MEDLINE  
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[PMID]:28456648
[Au] Autor:Al-Tawfiq JA; Memish ZA
[Ad] Endereço:Johns Hopkins Aramco Healthcare, Dhahran 31311, Saudi Arabia; Indiana University School of Medicine, Indianapolis, IN 46202, USA.
[Ti] Título:Alkhurma hemorrhagic fever virus.
[So] Source:Microbes Infect;19(6):305-310, 2017 06.
[Is] ISSN:1769-714X
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Alkhurma hemorrhagic fever virus (AHFV) was first isolated in Jeddah, Saudi Arabia, in the 1990s from the blood of a butcher. Subsequently, the virus was recognized in many patients in Saudi Arabia and rarely from Egypt and Djibouti. In this review, we summarize the current literature on AHFV globally with special focus on Saudi Arabia.
[Mh] Termos MeSH primário: Vírus da Encefalite Transmitidos por Carrapatos/isolamento & purificação
Encefalite Transmitida por Carrapatos/transmissão
[Mh] Termos MeSH secundário: Animais
Modelos Animais de Doenças
Vírus da Encefalite Transmitidos por Carrapatos/classificação
Encefalite Transmitida por Carrapatos/prevenção & controle
Seres Humanos
Arábia Saudita
Estações do Ano
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE


  4 / 2518 MEDLINE  
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[PMID]:27779467
[Au] Autor:Meltzer E; Paran Y; Lustig Y; Stienlauf S; Weinberger M; Schwartz E
[Ti] Título:Travel-Related Tick-Borne Encephalitis, Israel, 2006-2014.
[So] Source:Emerg Infect Dis;23(1):119-121, 2017 01.
[Is] ISSN:1080-6059
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:During 2006-2014, four tick-borne encephalitis (TBE) cases occurred among Israeli travelers. We calculated TBE incidence at 321.0, 45.0, 13.2, and 7.5 cases/100,000 travelers/year of travel to Sweden, Switzerland, Austria, and Germany, respectively. TBE incidence among travelers to these destinations appears to justify TBE vaccination in accordance with World Health Organization recommendations.
[Mh] Termos MeSH primário: Vetores Aracnídeos/virologia
Encefalite Transmitida por Carrapatos/epidemiologia
Encefalite Transmitida por Carrapatos/transmissão
Carrapatos/virologia
Viagem
[Mh] Termos MeSH secundário: Animais
Áustria/epidemiologia
Vírus da Encefalite Transmitidos por Carrapatos/patogenicidade
Vírus da Encefalite Transmitidos por Carrapatos/fisiologia
Encefalite Transmitida por Carrapatos/prevenção & controle
Alemanha/epidemiologia
Seres Humanos
Incidência
Israel/epidemiologia
Suécia/epidemiologia
Suíça/epidemiologia
Vacinação
Vacinas Virais/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Vaccines)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE
[do] DOI:10.3201/eid2301.160888


  5 / 2518 MEDLINE  
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[PMID]:29323856
[Au] Autor:Mel'nikova OV; Adel'shin RV; Korzun VM; Trushina YN; Andaev EI
[Ti] Título:Tick-borne encephalitis virus isolates from natural foci of the Irkutsk region: clarification of the genotype landscape.
[So] Source:Vopr Virusol;61(5):229-34, 2016.
[Is] ISSN:0507-4088
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:The Irkutsk region is the unique territory where all known subtypes of tick-borne encephalitis virus (TBEV) circulate. In the last years, the phenomenon of changes in TBEV subtypes (substitution of the Far-Eastern subtype by the Siberian one) was noted in some regions of the Russian Federation. The results of individual investigation of 11522 Ixodes persulcatus ticks and brain specimens from 81 small mammals collected in natural foci of the Irkutsk region during 2006-2014 are presented in the article. More than 60 TBEV strains have been isolated and studied by virological methods; E gene fragments (1193 b.p.) of 68 isolates have been typed. The majority of the strains (irrespective of subtype) were of high virulence for laboratory mice (LM) in case of both intracerebral and subcutaneous inoculation of virus. All isolates from warm-blooded small mammals and humans were of high virulence for LM, but placed in the same clusters of the phylogenetic tree with ticks collected in the same area. Tick-borne strains of different virulence also did not form separate clusters on the tree. Phylogenetic analysis showed that modern TBEV genotypic landscape of the studied territory is changing toward absolute predominance of the Siberian subtype (94.1%). This subtype is represented by two groups with prototype strains "Zausaev" and "Vasilchenko". The "Vasilchenko" group of strains is spread on the whole territory under study; the strains of "Zausaev" group were isolated previously in the Irkutsk suburbs. The European subtype of TBEV circulates in natural foci of Pribaikalie permanently (at least 5% of the random sampling); the strains are of high virulence for LM. The Far-Eastern TBEV subtype was not found within the group of isolates collected in 20062014. The phylogenetic relationship of the strains under study had a higher correlation with the place of isolation than with the year or source.
[Mh] Termos MeSH primário: Vírus da Encefalite Transmitidos por Carrapatos/genética
Encefalite Transmitida por Carrapatos/epidemiologia
Encefalite Transmitida por Carrapatos/veterinária
Genoma Viral
Genótipo
Ixodes/virologia
[Mh] Termos MeSH secundário: Animais
Vetores de Doenças
Vírus da Encefalite Transmitidos por Carrapatos/classificação
Vírus da Encefalite Transmitidos por Carrapatos/isolamento & purificação
Encefalite Transmitida por Carrapatos/transmissão
Encefalite Transmitida por Carrapatos/virologia
Feminino
Seres Humanos
Mamíferos/virologia
Camundongos
Filogenia
Sibéria/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE


  6 / 2518 MEDLINE  
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[PMID]:29323844
[Au] Autor:Pukhovskaya NM; Morozova OV; Belozerova NB; Bakhmetyeva SV; Vysochina NP; Zdanovskaya NI; Ivanov LI
[Ti] Título:Comparative analysis of genomes of tick-borne encephalitis virus strains isolated from mosquitoes and ticks.
[So] Source:Vopr Virusol;62(1):30-5, 2017.
[Is] ISSN:0507-4088
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:The tick-borne encephalitis virus (TBEV) strain Lazo MP36 was isolated from the pool of mosquitoes Aedes vexans collected in Lazo region of Khabarovsk territory in August 2014. Phylogenetic analysis of the strain Lazo MP36 complete genome (GenBank accession number KT001073) revealed its correspondence to the TBEV Far Eastern subtype and differences from the following strains: 1) from ticks Ixodes persulcatus P. Schulze, 1930 [vaccine strain 205 (JX498939) and strains Khekhtzir 1230 (KF880805), Chichagovka (KP844724), Birobidzhan 1354 (KF880805) isolated in 2012-2013]; 2) from mosquitoes [strain Malyshevo (KJ744034) isolated in 1978 from Aedes vexans nipponii in Khabarovsk territory; strain Sakhalin 6-11 isolated from the pool of mosquitoes in 2011 (KF826916)]; 3) from human brain [vaccine strain Sofjin (JN229223), Glubinnoe/2004(DQ862460). Kavalerovo (DQ862460), Svetlogorie (DQ862460)]. The fusion peptide necessary for flavivirus entry to cells of the three TBEV strains isolated from mosquitoes (Lazo MP36, Malyshevo and Sakhalin 6-11) has the canonical structure 98-DRGWGNHCGLFGKGSI-113 for the tick-borne flaviviruses. Amino acid transition H104G typical for the mosquito-borne flaviviruses was not found. Structures of 5'- and 3'-untranslated (UTR) regions of the TBEV strains from mosquitoes were 85-98% homologous to the TBEV strains of all subtypes without recombination with mosquito-borne flaviviruses found in the Far East of Russia. Secondary structures of 5'- and 3'-UTR as well as cyclization sequences (CS) of types a and B are highly homologous for all TBEV isolates independently of the biological hosts and vectors. similarity of the genomes of the TBEV isolates from mosquitoes, ticks and patients as well as pathogenicity of the isolates for new-borne laboratory mice and tissue cultures might suggest a possible role of mosquitoes in the TBEV circulation in natural foci as an accidental or additional virus carrier.
[Mh] Termos MeSH primário: Aedes/virologia
Vírus da Encefalite Transmitidos por Carrapatos/genética
Encefalite Transmitida por Carrapatos/epidemiologia
Genoma Viral
Ixodes/virologia
RNA Viral/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Animais Recém-Nascidos
Vetores de Doenças
Cães
Vírus da Encefalite Transmitidos por Carrapatos/classificação
Vírus da Encefalite Transmitidos por Carrapatos/isolamento & purificação
Encefalite Transmitida por Carrapatos/transmissão
Encefalite Transmitida por Carrapatos/virologia
Extremo Oriente/epidemiologia
Genótipo
Seres Humanos
Camundongos
Camundongos Endogâmicos ICR
Conformação de Ácido Nucleico
Filogenia
RNA Viral/química
RNA Viral/isolamento & purificação
Roedores/virologia
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Sibéria/epidemiologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE


  7 / 2518 MEDLINE  
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[PMID]:28915259
[Au] Autor:Haslwanter D; Blaas D; Heinz FX; Stiasny K
[Ad] Endereço:Center for Virology, Medical University of Vienna, Vienna, Austria.
[Ti] Título:A novel mechanism of antibody-mediated enhancement of flavivirus infection.
[So] Source:PLoS Pathog;13(9):e1006643, 2017 Sep.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Antibody-dependent enhancement of viral infection is a well-described phenomenon that is based on the cellular uptake of infectious virus-antibody complexes following their interaction with Fcγ receptors expressed on myeloid cells. Here we describe a novel mechanism of antibody-mediated enhancement of infection by a flavivirus (tick-borne encephalitis virus) in transformed and primary human cells, which is independent of the presence of Fcγ receptors. Using chemical cross-linking and immunoassays, we demonstrate that the monoclonal antibody (mab) A5, recognizing an epitope at the interface of the dimeric envelope protein E, causes dimer dissociation and leads to the exposure of the fusion loop (FL). Under normal conditions of infection, this process is triggered only after virus uptake by the acidic pH in endosomes, resulting in the initiation of membrane fusion through the interaction of the FL with the endosomal membrane. Analysis of virus binding and cellular infection, together with inhibition by the FL-specific mab 4G2, indicated that the FL, exposed after mab A5- induced dimer-dissociation, mediated attachment of the virus to the plasma membrane also at neutral pH, thereby increasing viral infectivity. Since antibody-induced enhancement of binding was not only observed with cells but also with liposomes, it is likely that increased infection was due to FL-lipid interactions and not to interactions with cellular plasma membrane proteins. The novel mechanism of antibody-induced infection enhancement adds a new facet to the complexity of antibody interactions with flaviviruses and may have implications for yet unresolved effects of polyclonal antibody responses on biological properties of these viruses.
[Mh] Termos MeSH primário: Anticorpos Antivirais/imunologia
Anticorpos Facilitadores
Vírus da Encefalite Transmitidos por Carrapatos/imunologia
Infecções por Flavivirus/virologia
Flavivirus/imunologia
[Mh] Termos MeSH secundário: Anticorpos Monoclonais/imunologia
Anticorpos Facilitadores/imunologia
Flavivirus/isolamento & purificação
Seres Humanos
Lipossomos/imunologia
Proteínas do Envelope Viral/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Antibodies, Viral); 0 (Liposomes); 0 (Viral Envelope Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006643


  8 / 2518 MEDLINE  
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[PMID]:28791846
[Au] Autor:Kepka A; Janas RM; Pancewicz SA; Swierzbinska R
[Ad] Endereço:Department of Biochemistry, Radioimmunology and Experimental Medicine, the Children's Memorial Health Institute, Warszawa, Poland.
[Ti] Título:Serum carnitine and acyl-carnitine in patients with meningitis due to tick-borne encephalitis virus infection.
[So] Source:Adv Clin Exp Med;26(2):277-280, 2017 Mar-Apr.
[Is] ISSN:1899-5276
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Hard ticks are the main vectors of tick-borne encephalitis virus (TBEV). Free carnitine (FC) and acylcarnitines (AC) have the basic role in ß-oxidation as well as the modulation of immune and nervous system. Homeostasis of carnitines in the TBE patients was not studied so far. OBJECTIVES: This study aimed to evaluate FC and AC serum concentrations in patients with meningitis due to TBEV infection before and after 14 ± 3 days of treatment. MATERIAL AND METHODS: The study was performed in 14 patients aged 48 ± 29 years that were divided a posteriori (based on their FC level before and after treatment) into 2 subgroups: 1-8 and 9-14. Diagnosis was based on the neurological, serological and pleocytosis evaluation. RESULTS: The FC level in patients 1-8 before treatment (24.1 ± 8.1) was significantly lower than in patients post-treatment (34.4 ± 8.3), lower than in the control group (40.5 ± 7.6), and lower than in patients 9-14 before treatment (40.0 ± 13.5) but not lower than in the patients 9-14 after treatment (24.7 ± 7.3 µmol/L), respectively, p < 0.05. AC concentration in the patients 1-8 before treatment (4.7 ± 2.2) was apparently lower than in patients post-treatment (9.5 ± 3.9 µmol/L) but the values were not significantly different. In patients 9-14 before treatment the AC concentration (16.3 ± 12.6) was higher than in patients after treatment (5.3 ± 4.0 µmol/L), but the difference was not statistically significant. CONCLUSIONS: FC and AC homeostasis in circulation was disturbed in the patients with meningitis due to TBEV infection patients. The mean levels of FC and AC in 60% of the patients were below the normal range but normalized after treatment whereas in 40% of the patients they were near or at a normal range and significantly decreased after treatment. Explanation of this intriguing finding and its clinical significance is not easy without further studies.
[Mh] Termos MeSH primário: Carnitina/análogos & derivados
Carnitina/sangue
Vírus da Encefalite Transmitidos por Carrapatos/isolamento & purificação
Encefalite Transmitida por Carrapatos/sangue
Meningite/sangue
[Mh] Termos MeSH secundário: Adulto
Idoso
Animais
Anticorpos Antivirais/sangue
Anticorpos Antivirais/imunologia
Vírus da Encefalite Transmitidos por Carrapatos/imunologia
Vírus da Encefalite Transmitidos por Carrapatos/fisiologia
Encefalite Transmitida por Carrapatos/complicações
Encefalite Transmitida por Carrapatos/virologia
Feminino
Seres Humanos
Imunoglobulina G/sangue
Imunoglobulina G/imunologia
Imunoglobulina M/sangue
Imunoglobulina M/imunologia
Masculino
Meningite/complicações
Meningite/terapia
Meia-Idade
Resultado do Tratamento
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Immunoglobulin G); 0 (Immunoglobulin M); 0 (acylcarnitine); S7UI8SM58A (Carnitine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170908
[Lr] Data última revisão:
170908
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.17219/acem/63006


  9 / 2518 MEDLINE  
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[PMID]:28786780
[Au] Autor:Selinger M; Wilkie GS; Tong L; Gu Q; Schnettler E; Grubhoffer L; Kohl A
[Ad] Endereço:1​Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, Branisovská 31, 370 05 Ceské Budejovice, Czech Republic 2​Faculty of Science, University of South Bohemia in Ceské Budejovice, Branisovská 31, 370 05 Ceské Budejovice, Czech Republic.
[Ti] Título:Analysis of tick-borne encephalitis virus-induced host responses in human cells of neuronal origin and interferon-mediated protection.
[So] Source:J Gen Virol;98(8):2043-2060, 2017 Aug.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tick-borne encephalitis virus (TBEV) is a member of the genus Flavivirus. It can cause serious infections in humans that may result in encephalitis/meningoencephalitis. Although several studies have described the involvement of specific genes in the host response to TBEV infection in the central nervous system (CNS), the overall network remains poorly characterized. Therefore, we investigated the response of DAOY cells (human medulloblastoma cells derived from cerebellar neurons) to TBEV (Neudoerfl strain, Western subtype) infection to characterize differentially expressed genes by transcriptome analysis. Our results revealed a wide panel of interferon-stimulated genes (ISGs) and pro-inflammatory cytokines, including type III but not type I (or II) interferons (IFNs), which are activated upon TBEV infection, as well as a number of non-coding RNAs, including long non-coding RNAs. To obtain a broader view of the pathways responsible for eliciting an antiviral state in DAOY cells we examined the effect of type I and III IFNs and found that only type I IFN pre-treatment inhibited TBEV production. The cellular response to TBEV showed only partial overlap with gene expression changes induced by IFN-ß treatment - suggesting a virus-specific signature - and we identified a group of ISGs that were highly up-regulated following IFN-ß treatment. Moreover, a high rate of down-regulation was observed for a wide panel of pro-inflammatory cytokines upon IFN-ß treatment. These data can serve as the basis for further studies of host-TBEV interactions and the identification of ISGs and/or lncRNAs with potent antiviral effects in cases of TBEV infection in human neuronal cells.
[Mh] Termos MeSH primário: Citocinas/genética
Vírus da Encefalite Transmitidos por Carrapatos/fisiologia
Encefalite Transmitida por Carrapatos/genética
Encefalite Transmitida por Carrapatos/virologia
Interferons/genética
[Mh] Termos MeSH secundário: Citocinas/imunologia
Vírus da Encefalite Transmitidos por Carrapatos/genética
Encefalite Transmitida por Carrapatos/imunologia
Interações Hospedeiro-Patógeno
Seres Humanos
Interferons/imunologia
Neurônios/imunologia
Neurônios/virologia
Ativação Transcricional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 9008-11-1 (Interferons)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170809
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000853


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[PMID]:28705180
[Au] Autor:Ai J; Xie Z; Liu G; Chen Z; Yang Y; Li Y; Chen J; Zheng G; Shen K
[Ad] Endereço:Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Virology Laboratory, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing,
[Ti] Título:Etiology and prognosis of acute viral encephalitis and meningitis in Chinese children: a multicentre prospective study.
[So] Source:BMC Infect Dis;17(1):494, 2017 Jul 14.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In China, there were few studies about the pathogens of acute viral encephalitis and meningitis in children in recent years. The aims of this study were to characterize the etiology and prognosis of acute viral encephalitis and meningitis in Chinese children. METHODS: This was a multicentre prospective study. Two hundred and sixty one viral encephalitis patients and 285 viral meningitis patients were enrolled. The mean age of viral encephalitis and meningitis were 5.88 ± 3.60 years and 6.39 ± 3.57 years, respectively. Real-time reverse transcription PCR and multiplex PCR were used to detect human enteroviruses and herpes viruses in cerebrospinal fluid (CSF) of patients with encephalitis or meningitis. The enzyme-linked immune absorbent assay (ELISA) was used for detecting IgM antibody against Japanese encephalitis virus (JEV) in CSF and against mumps virus, tick-borne encephalitis virus (TBEV), dengue virus and rubella virus in acute serum. The clinical and outcome data were collected during patients' hospitalization. RESULTS: The etiology of viral encephalitis was confirmed in 52.5% patients. The primary pathogen was human enteroviruses (27.7%) in viral encephalitis. The incidence of sequelae and the fatality rate of viral encephalitis with confirmed etiology were 7.5% and 0.8%, respectively. The etiology of viral meningitis was identified in 42.8% cases. The leading pathogen was also human enteroviruses (37.7%) in viral meningitis. The prognosis of viral meningitis was favorable with only 0.7% patients had neurological sequelae. CONCLUSIONS: Human enteroviruses were the leading cause both in acute viral encephalitis and viral meningitis in children. The incidence of sequelae and fatality rate of viral encephalitis with confirmed etiology were 7.5% and 0.8%, respectively. The prognosis of viral meningitis was favorable compared to viral encephalitis.
[Mh] Termos MeSH primário: Encefalite Viral/etiologia
Meningite Viral/etiologia
[Mh] Termos MeSH secundário: Adolescente
Criança
Pré-Escolar
China/epidemiologia
Vírus da Encefalite Japonesa (Espécie)/imunologia
Vírus da Encefalite Japonesa (Espécie)/patogenicidade
Vírus da Encefalite Transmitidos por Carrapatos/imunologia
Vírus da Encefalite Transmitidos por Carrapatos/patogenicidade
Encefalite Viral/epidemiologia
Enterovirus/genética
Enterovirus/imunologia
Enterovirus/patogenicidade
Feminino
Seres Humanos
Incidência
Lactente
Masculino
Meningite Viral/epidemiologia
Reação em Cadeia da Polimerase Multiplex
Prognóstico
Estudos Prospectivos
Vírus da Rubéola/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170715
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2572-9



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