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  1 / 2027 MEDLINE  
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[PMID]:28450492
[Ti] Título:Mucosal disease in a group of persistently infected cattle.
[So] Source:Vet Rec;180(17):429, 2017 04 29.
[Is] ISSN:2042-7670
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Doença das Mucosas por Vírus da Diarreia Viral Bovina
Vírus da Diarreia Viral Bovina/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais
Bovinos
Doenças dos Bovinos
[Pt] Tipo de publicação:LETTER; COMMENT
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180109
[Lr] Data última revisão:
180109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1136/vr.j2064


  2 / 2027 MEDLINE  
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[PMID]:28874234
[Au] Autor:Radtke C; Tews BA
[Ad] Endereço:Institute of Immunology, Friedrich-Loeffler-Institut, Federal Research Institute of Animal Health, Südufer 10, 17493 Greifswald - Insel Riems, Germany.
[Ti] Título:Retention and topology of the bovine viral diarrhea virus glycoprotein E2.
[So] Source:J Gen Virol;98(10):2482-2494, 2017 Oct.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pestiviruses are enveloped viruses that bud intracellularly. They have three envelope glycoproteins, E , E1, and E2. E2 is the receptor binding protein and the main target for neutralizing antibodies. Both E and E2 are retained intracellularly. Here, E2 of the bovine viral diarrhea virus (BVDV) strain CP7 was used to study the membrane topology and intracellular localization of the protein. E2 is localized in the ER and there was no difference between E2 expressed alone or in the context of the viral polyprotein. The mature E2 protein was found to possess a single span transmembrane anchor. For the mapping of a retention signal CD72-E2 fusion proteins, as well as E2 alone were analysed. This confirmed the importance of the transmembrane domain and arginine 355 for intracellular retention, but also revealed a modulating effect on retention through the cytoplasmic tail of the E2 protein, especially through glutamine 370. Mutants with a strong impact on retention were tested in the viral context and we were able to rescue BVDV with certain mutations that in E2 alone impaired intracellular retention and lead to export of E2 to the cells surface.
[Mh] Termos MeSH primário: Anticorpos Antivirais/imunologia
Vírus da Diarreia Viral Bovina/genética
Vírus da Diarreia Viral Bovina/imunologia
Proteínas do Envelope Viral/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Anticorpos Neutralizantes/imunologia
Células CHO
Bovinos
Linhagem Celular
Cricetinae
Cricetulus
Estrutura Secundária de Proteína
Coelhos
Proteínas do Envelope Viral/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Neutralizing); 0 (Antibodies, Viral); 0 (Viral Envelope Proteins); 0 (glycoprotein E2, bovine viral diarrhea virus)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000912


  3 / 2027 MEDLINE  
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[PMID]:28854799
[Au] Autor:Nikolaeva-Glomb L; Mukova L; Nikolova N; Kussovski V; Doumanova L; Mantareva V; Angelov I; Wöhrle D; Galabov AS
[Ti] Título:Photodynamic Effect of some Phthalocyanines on Enveloped and Naked Viruses.
[So] Source:Acta Virol;61(3):341-346, 2017.
[Is] ISSN:0001-723X
[Cp] País de publicação:Slovakia
[La] Idioma:eng
[Ab] Resumo:Activity of three photosensitizing phthalocyanine derivatives was tested for photodynamic inactivation towards two coated and two non-enveloped viruses - bovine viral diarrhea virus (BVDV), influenza virus A(H3N2), poliovirus type 1 (PV-1) and human adenovirus type 5 (HAdV5). In the case of coated viruses, a combination of virucidal and irradiation effects was registered by octa-methylpyridyloxy-substituted Ga phthalocyanine (Ga8) toward BVDV, whereas tetra-methylpyridyloxy-substituted Ga phthalocyanine (Ga4) revealed a marked photoinactivation only. No such effect was observed towards influenza A virus. In contrast, the photoinactivating potential of Ga4 and Ga8 marked very high values on naked viruses, especially on HAdV5, at which both the virucidal as well as the irradiation effects became combined.
[Mh] Termos MeSH primário: Adenovírus Humanos/efeitos dos fármacos
Vírus da Diarreia Viral Bovina/efeitos dos fármacos
Indóis/farmacologia
Vírus da Influenza A Subtipo H3N2/efeitos dos fármacos
Fármacos Fotossensibilizantes/farmacologia
Poliovirus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Bovinos
Linhagem Celular
Cães
Seres Humanos
Células Madin Darby de Rim Canino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indoles); 0 (Photosensitizing Agents); V5PUF4VLGY (phthalocyanine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.4149/av_2017_313


  4 / 2027 MEDLINE  
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[PMID]:28688346
[Au] Autor:Falkenberg SM; Dassanayake RP; Neill JD; Ridpath JF
[Ad] Endereço:Ruminant Disease and Immunology Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, United States. Electronic address: Shollie.Falkenberg@ars.usda.gov.
[Ti] Título:Improved detection of bovine viral diarrhea virus in bovine lymphoid cell lines using PrimeFlow RNA assay.
[So] Source:Virology;509:260-265, 2017 Sep.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bovine viral diarrhea virus (BVDV) infections, whether as acute, persistent or contributing to co-infections, result in significant losses for cattle producers. Although, BVDV can be identified readily by real-time PCR and ELISA, detection and quantification of viral infection at the single cell level is extremely difficult. Detection at the single lymphoid cell level is important due to the immunomodulation that accompanies BVDV infection. A novel PrimeFlow RNA assay using in-situ detection of BVDV was evaluated. The model used to develop this technique included three BL-3 cell lines with different infection statuses, one not infected with BVDV, one infected with BVDV and one dual infected with BVDV and bovine leukosis virus. Using RNA probes specific for the BVDV-2a N -E coding region, BVDV RNA was detected from both contaminated BL-3 cell lines by flow cytometry and fluorescent microscopy. This is the first report on in-situ detection of BVDV at the single-cell level.
[Mh] Termos MeSH primário: Vírus da Diarreia Viral Bovina/isolamento & purificação
Citometria de Fluxo/métodos
Linfócitos/virologia
Microscopia de Fluorescência/métodos
RNA Viral/análise
Análise de Célula Única/métodos
[Mh] Termos MeSH secundário: Animais
Bovinos
Vírus da Diarreia Viral Bovina/genética
Virologia/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170721
[Lr] Data última revisão:
170721
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170709
[St] Status:MEDLINE


  5 / 2027 MEDLINE  
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[PMID]:28671068
[Au] Autor:Laureyns J
[Ad] Endereço:Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. Electronic address: Jozef.Laureyns@UGent.be.
[Ti] Título:What are the monetary losses by BVDV infection and is control cost-effective?
[So] Source:Vet J;223:32-33, 2017 05.
[Is] ISSN:1532-2971
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Doença das Mucosas por Vírus da Diarreia Viral Bovina
Vírus da Diarreia Viral Bovina
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:EDITORIAL; COMMENT
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170815
[Lr] Data última revisão:
170815
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE


  6 / 2027 MEDLINE  
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[PMID]:28668732
[Au] Autor:Chernick A; van der Meer F
[Ad] Endereço:Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, 3330 Hospital Dr NW, Calgary, AB T2N 1N4, Canada. Electronic address: a.chernick@ucalgary.ca.
[Ti] Título:Evolution of Bovine viral diarrhea virus in Canada from 1997 to 2013.
[So] Source:Virology;509:232-238, 2017 Sep.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bovine viral diarrhea virus (BVDV) is a rapidly evolving, single-stranded RNA virus and a production limiting pathogen of cattle worldwide. 79 viral isolates collected between 1997 and 2013 in Canada were subjected to next-generation sequencing. Bayesian phylogenetics was used to assess the evolution of this virus. A mean substitution rate of 1.4×10 substitutions/site/year was found across both BVDV1 and BVDV2. Evolutionary rates in the E2 gene were slightly faster than other regions. We also identified population structures below the sub-genotype level that likely have phenotypic implications. Two distinct clusters within BVDV2a are present and can be differentiated, in part, by a tyrosine to isoleucine mutation at position 963 in the E2 protein, a position implicated in the antigenicity of BVDV1 isolates. Distinct clustering within all sub-genotypes, particularly BVDV2a, is apparent and could lead to new levels of genotypic classification. Continuous monitoring of emerging variants is therefore necessary.
[Mh] Termos MeSH primário: Doença das Mucosas por Vírus da Diarreia Viral Bovina/epidemiologia
Doença das Mucosas por Vírus da Diarreia Viral Bovina/virologia
Vírus da Diarreia Viral Bovina/classificação
Vírus da Diarreia Viral Bovina/genética
Evolução Molecular
Variação Genética
[Mh] Termos MeSH secundário: Substituição de Aminoácidos
Animais
Canadá/epidemiologia
Bovinos
Análise por Conglomerados
Biologia Computacional
Vírus da Diarreia Viral Bovina/isolamento & purificação
Genótipo
Sequenciamento de Nucleotídeos em Larga Escala
Taxa de Mutação
Filogenia
RNA Viral/genética
Análise de Sequência de DNA
Homologia de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170721
[Lr] Data última revisão:
170721
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170703
[St] Status:MEDLINE


  7 / 2027 MEDLINE  
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[PMID]:28582444
[Au] Autor:Li T; Huang M; Xiao H; Zhang G; Ding J; Wu P; Zhang H; Sheng J; Chen C
[Ad] Endereço:College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
[Ti] Título:Selection and characterization of specific nanobody against bovine virus diarrhea virus (BVDV) E2 protein.
[So] Source:PLoS One;12(6):e0178469, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bovine viral diarrhea-mucosal disease (BVD-MD) is caused by bovine viral diarrhea virus (BVDV), and results in abortion, stillbirth, and fetal malformation in cows. Here, we constructed the phage display vector pCANTAB 5E-VHH and then transformed it into Escherichia coli TG1-competent cells, to construct an initial anti-BVDV nanobody gene library. We obtained a BVDV-E2 antigen epitope bait protein by prokaryotic expression using the nucleotide sequence of the E2 gene of the BVDV-NADL strain published in GenBank. Phage display was used to screen the anti-BVDV nanobody gene library. We successfully constructed a high quality phage display nanobody library, with an initial library capacity of 4.32×105. After the rescue of helper phage, the titer of the phage display nanobody library was 1.3×1011. The BVDV-E2 protein was then expressed in Escherichia coli (DE3), and a 49.5 kDa band was observed with SDS-PAGE analysis that was consistent with the expected nanobody size. Thus, we were able to isolate one nanobody that exhibits high affinity and specificity against BVDV using phage display techniques. This isolated nanobody was then used in Enzyme Linked Immunosorbent Assay and qRT-PCR, and ELISA analyses of BVDV infection of MDBK cells indicated that the nanobodies exhibited good antiviral effect.
[Mh] Termos MeSH primário: Anticorpos Antivirais/biossíntese
Antígenos Virais/genética
Vírus da Diarreia Viral Bovina/genética
Epitopos/genética
Anticorpos de Domínio Único/biossíntese
Proteínas do Envelope Viral/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Anticorpos Antivirais/genética
Afinidade de Anticorpos
Especificidade de Anticorpos
Antígenos Virais/imunologia
Sequência de Bases
Bovinos
Linhagem Celular
Vírus da Diarreia Viral Bovina/imunologia
Ensaio de Imunoadsorção Enzimática
Células Epiteliais/imunologia
Células Epiteliais/patologia
Células Epiteliais/virologia
Epitopos/imunologia
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Rim/imunologia
Rim/patologia
Rim/virologia
Biblioteca de Peptídeos
Proteínas Recombinantes/genética
Proteínas Recombinantes/imunologia
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Anticorpos de Domínio Único/genética
Proteínas do Envelope Viral/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Antigens, Viral); 0 (Epitopes); 0 (Peptide Library); 0 (Recombinant Proteins); 0 (Single-Domain Antibodies); 0 (Viral Envelope Proteins); 0 (gp53, bovine viral diarrhea virus)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170606
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178469


  8 / 2027 MEDLINE  
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[PMID]:28537239
[Au] Autor:Ghassemi F; Madadgar O; Roohvand F; Rasekhian M; Etemadzadeh MH; Boroujeni GRN; Langroudi AG; Azadmanesh K
[Ad] Endereço:Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
[Ti] Título:[Translational efficiency of BVDV IRES and EMCV IRES for T7 RNA polymerase driven cytoplasmic expression in mammalian cell lines].
[So] Source:Mol Biol (Mosk);51(2):324-333, 2017 Mar-Apr.
[Is] ISSN:0026-8984
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Mammalian T7 polymerase-based cytoplasmic expression systems are common tool for molecular studies. The majority of these systems include the internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV). To carry out a cap-independent translation process, this type of IRES might require the expression of an extensive array of host factors, what is a disadvantage. Other IRESes might be less dependent on the host cell factors, but their biology is characterized to a lesser degree. Here, we compare the translational efficiencies of bovine viral diarrhea virus (BVDV) IRES with that of ECMV. Both IRESes were tested in reporter vectors containing the T7 promoter, an IRES of choice and the coding sequence of the enhanced green fluorescent protein (EGFP). To provide for the expression of T7 RNA polymerase, the corresponding gene was isolated from Escherichia coli and inserted into pCDNA3.1-Hygro(+). After co-transfection of the T7 RNA polymerase encoding vector with either of the two IRES-containing reporter vectors into T7 baby hamster kidney (T7-BHK), human embryonic kidney (HEK) 293T, chinese hamster ovary (CHO) and HeLa cells, the translational efficiency of the reporter construct was studied by fluorescence microscopy and flow cytometry. In T7-BHK, HEK 293T and HeLa cells the translational efficiency of BVDV IRES was two to three times higher than that of EMCV IRES. In CHO cells, BVDV IRES and EMCV IRES were equally efficient. An analysis of the secondary structure of respective mRNAs showed that their ΔG values were -544.00 and -469.40 kcal/mol for EMCV IRES and BVDV IRES harboring molecules, respectively. As EMCV IRES-containing mRNA is more stable, it is evident that other, still unidentified factors should be held responsible for the enhanced translational efficiency of BDVD IRES. Taken together, our results indicate the potential of BVDV IRES as a replacement for EMCV IRES, which is now commonly used for T7 polymerase driven cytoplasmic expression of genes of interest or virus cDNA rescue experiments.
[Mh] Termos MeSH primário: Citoplasma/metabolismo
RNA Polimerases Dirigidas por DNA/metabolismo
Vírus da Diarreia Viral Bovina/genética
Vírus da Encefalomiocardite/genética
Expressão Gênica
Sítios Internos de Entrada Ribossomal
Proteínas Virais/metabolismo
[Mh] Termos MeSH secundário: Citoplasma/genética
RNA Polimerases Dirigidas por DNA/genética
Células HEK293
Células HeLa
Seres Humanos
Proteínas Recombinantes/biossíntese
Proteínas Recombinantes/genética
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Internal Ribosome Entry Sites); 0 (Recombinant Proteins); 0 (Viral Proteins); EC 2.7.7.- (bacteriophage T7 RNA polymerase); EC 2.7.7.6 (DNA-Directed RNA Polymerases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170525
[St] Status:MEDLINE
[do] DOI:10.7868/S0026898417020112


  9 / 2027 MEDLINE  
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[PMID]:28532992
[Au] Autor:Byrne AW; Guelbenzu-Gonzalo M; Strain SAJ; McBride S; Graham J; Lahuerta-Marin A; Harwood R; Graham DA; McDowell S
[Ad] Endereço:Agri-Food and Biosciences Institute, Veterinary Science Division, Stormont, Belfast BT43SD, United Kingdom; School of Biological Sciences, Queen's University Belfast, Belfast, United Kingdom. Electronic address: andrew.byrne@afbini.gov.uk.
[Ti] Título:Assessment of concurrent infection with bovine viral diarrhoea virus (BVDV) and Mycobacterium bovis: A herd-level risk factor analysis from Northern Ireland.
[So] Source:Prev Vet Med;141:38-47, 2017 Jun 01.
[Is] ISSN:1873-1716
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Bovine viral diarrhoea virus (BVDV) is a significant pathogen of cattle, leading to severe economic and animal-welfare impacts. Furthermore, the pathogen has been associated with impacting the progression or spread of other pathogens (e.g. Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB)). During this study we investigated (i) risk factors for BVDV at a herd-level and (ii) whether there was any association between BVDV and herd-level bTB risk. The data for this study were gathered from a voluntary BVDV control programme in Northern Ireland (2013-2015) based on the identification of virus positive animals through tissue tag testing of calves. We assigned a herd-level BVDV status to 2827 participating herds, where a herd was assumed "infected" if one or more animals tested positive for BVDV. Two model suites were developed. Firstly, we assessed risk factors for BVDV herd status using multivariable logit random-effects modelling, aggregating to the calendar year level (2013-2015; n=4828; model 1). Secondly, we aggregated data across the three years of the study to give an overall status for the whole study period (n=2827; logistic model 2). Risk factors included year, herd-type, herd size, number of births, inward trade moves, calf mortality, and region. Furthermore, the herd-level bovine tuberculosis status (based on the single intradermal comparative cervical tuberculin (SICCT) test outcomes, or confirmation at post-mortem), or the size of bTB breakdowns (number of SICCT test positive animals), of herds was also investigated to assess whether there was an association (co-infection) with herd BVDV status. The final models suggested that BVDV herd status was positively associated with increased levels of calf mortality, herd size, number of births, the number of BVDV tests undertaken and the number of animals introduced to the herd. There was a significant univariable positive association between BVDV status, and SICCT breakdown risk, breakdown size and confirmed bTB status in model 2. However, there was no evidence of significant associations between bTB status (using SICTT status, confirmed status or herd breakdown size) and BVDV status in final multivariable models when controlling for other significant confounders. These results provide information for action for the future control and eradication of BVDV in Northern Ireland, though these data provide little support for the hypothesised association between BVDV and bTB status at herd-level. Further animal-level analyses are necessary to investigate whether there is support for a BVD-bTB co-infection association, including the impact of co-infection on the severity of infection.
[Mh] Termos MeSH primário: Doença das Mucosas por Vírus da Diarreia Viral Bovina/complicações
Doenças dos Bovinos
Coinfecção/veterinária
Tuberculose Bovina/complicações
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos/microbiologia
Doenças dos Bovinos/virologia
Coinfecção/microbiologia
Coinfecção/virologia
Indústria de Laticínios
Vírus da Diarreia Viral Bovina
Feminino
Irlanda
Masculino
Mycobacterium bovis
Fatores de Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170524
[St] Status:MEDLINE


  10 / 2027 MEDLINE  
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[PMID]:28494925
[Au] Autor:Platt R; Kesl L; Guidarini C; Wang C; Roth JA
[Ad] Endereço:College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
[Ti] Título:Comparison of humoral and T-cell-mediated immune responses to a single dose of Bovela live double deleted BVDV vaccine or to a field BVDV strain.
[So] Source:Vet Immunol Immunopathol;187:20-27, 2017 May.
[Is] ISSN:1873-2534
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to determine and compare the humoral and cellular immune responses of calves exposed to a single dose of Bovela bovine viral diarrhea virus (BVDV) live double deleted vaccine or a field strain virus (FSV) of BVDV type 2 (strain 890). Thirty seronegative, colostrum-deprived 5 month-old Holstein steer calves that tested negative for persistent BVDV by ear notch immunohistochemistry and seronegative to BVDV types 1 and 2 were used. Calves were screened by multi-parameter flow cytometry (MP-FCM) 1 week before vaccination to ensure that they were negative for T cell responses to the BVDV types 1 and 2 viruses in the Bovela vaccine. Calves were assigned to 3 treatment groups: control (PBS), FSV inoculated, and Bovela vaccinated. The humoral response was tested by standard serum virus neutralization (SVN) test to BVDV types 1 (Singer strain) and 2 (strain 125). The response by CD4, CD8, and gamma delta (γδ TCR) T cells was evaluated by MP-FCM using individual BVDV types 1 and 2 from Bovela vaccine as recall antigens at 5, 6, and 7 weeks after vaccination. Activation markers used were upregulation of surface CD25 (IL-2R), intracellular interferon gamma (IFNγ) and intracellular interleukin 4 (IL-4). Each T cell subset was evaluated for increased expression of each activation marker compared to non-antigen stimulated cells of the same animal. All Bovela vaccinated and FSV inoculated calves produced SVN antibodies to both BVDV types 1 and 2 while control animals remained seronegative throughout the study. The mean (weeks 5, 6, and 7) T cell recall responses to Bovela BVDV type 1 and type 2 recall antigens were numerically higher in all three T cell subsets (CD4, CD8, and γδ TCR) for all three activation markers (CD25, IFNγ, and IL-4) when compared to either the control animals or to the FSV inoculated animals. These differences were often, but not always, statistically significant (P<0.05).
[Mh] Termos MeSH primário: Doença das Mucosas por Vírus da Diarreia Viral Bovina/prevenção & controle
Vírus da Diarreia Viral Bovina/imunologia
Vacinas Virais/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/imunologia
Doença das Mucosas por Vírus da Diarreia Viral Bovina/imunologia
Bovinos
Vírus da Diarreia Viral Bovina Tipo 1/imunologia
Vírus da Diarreia Viral Bovina Tipo 2/imunologia
Imunidade Celular
Masculino
Testes de Neutralização/veterinária
Linfócitos T/imunologia
Vacinas Virais/imunologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Viral Vaccines)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE



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