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[PMID]: 27775612 [Au] Autor: von Nordheim M; Boinay M; Leisi R; Kempf C; Ros C [Ad] Endereço: Department of Chemistry and Biochemistry, University of Bern, Bern 3012, Switzerland. marcus.vonnordheim@dcb.unibe.ch. [Ti] Título: Cutthroat Trout Virus-Towards a Virus Model to Support Hepatitis E Research. [So] Source: Viruses;8(10), 2016 10 20. [Is] ISSN: 1999-4915 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: Cutthroat trout virus (CTV) is a non-pathogenic fish virus belonging to the family, and it is distantly related to hepatitis E virus (HEV). Here, we report the development of an efficient cell culture system where CTV can consistently replicate to titers never observed before with a hepevirus. By using the rainbow trout gill (RTGill-W1) cell line, CTV reaches 10 geq/mL intracellularly and 108 geq/mL extracellularly within 5-6 days in culture. We additionally established a qPCR system to investigate CTV infectivity, and developed a specific antibody directed against the viral capsid protein encoded by ORF2. With these methods, we were able to follow the progressive accumulation of viral RNA and the capsid protein, and their intracellular distribution during virus replication. Virus progeny purified through iodixanol density gradients indicated-that similar to HEV-CTV produced in cell culture is also lipid-associated. The lack of an efficient cell culture system has greatly impeded studies with HEV, a major human pathogen that causes hepatitis worldwide. Although several cell culture systems have recently been established, the replication efficiency of HEV is not robust enough to allow studies on different aspects of the virus replication cycle. Therefore, a surrogate virus that can replicate easily and efficiently in cultured cells would be helpful to boost research studies with hepeviruses. Due to its similarities, but also its key differences to HEV, CTV represents a promising tool to elucidate aspects of the replication cycle of in general, and HEV in particular. [Mh] Termos MeSH primário: Hepevirus/fisiologia Cultura de Vírus/métodos Replicação Viral [Mh] Termos MeSH secundário: Animais Antígenos Virais/análise Linhagem Celular Imunoensaio/métodos Oncorhynchus mykiss RNA Viral/análise Reação em Cadeia da Polimerase em Tempo Real [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Antigens, Viral); 0 (RNA, Viral) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 171212 [Lr] Data última revisão: 171212 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161025 [St] Status: MEDLINE

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[PMID]: 29022866 [Au] Autor: Purdy MA; Harrison TJ; Jameel S; Meng XJ; Okamoto H; Van der Poel WHM; Smith DB; Ictv Report Consortium [Ad] Endereço: 1​Division of Viral Hepatitis, Centers for Disease Control and Prevention, MS-A33, 1600 Clifton Rd NE, Atlanta, GA 30329, USA. [Ti] Título: ICTV Virus Taxonomy Profile: Hepeviridae. [So] Source: J Gen Virol;98(11):2645-2646, 2017 Nov. [Is] ISSN: 1465-2099 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The family Hepeviridae includes enterically transmitted small non-enveloped positive-sense RNA viruses. It includes the genera Piscihepevirus, whose members infect fish, and Orthohepevirus, whose members infect mammals and birds. Members of the genus Orthohepevirus include hepatitis E virus, which is responsible for self-limiting acute hepatitis in humans and several mammalian species; the infection may become chronic in immunocompromised individuals. Extrahepatic manifestations of Guillain-Barré syndrome, neuralgic amyotrophy, glomerulonephritis and pancreatitis have been described in humans. Avian hepatitis E virus causes hepatitis-splenomegaly syndrome in chickens. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Hepeviridae, which is available at www.ictv.global/report/hepeviridae. [Mh] Termos MeSH primário: Hepatite Viral Animal/virologia Hepatite Viral Humana/virologia Hepevirus/classificação [Mh] Termos MeSH secundário: Animais Seres Humanos [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171113 [Lr] Data última revisão: 171113 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171013 [St] Status: MEDLINE [do] DOI: 10.1099/jgv.0.000940

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[PMID]: 28586923 [Au] Autor: Kandathil AJ; Breitwieser FP; Sachithanandham J; Robinson M; Mehta SH; Timp W; Salzberg SL; Thomas DL; Balagopal A [Ad] Endereço: From Johns Hopkins University and Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland. [Ti] Título: Presence of Human Hepegivirus-1 in a Cohort of People Who Inject Drugs. [So] Source: Ann Intern Med;167(1):1-7, 2017 Jul 04. [Is] ISSN: 1539-3704 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Background: Next-generation metagenomic sequencing (NGMS) has opened new frontiers in microbial discovery but has been clinically characterized in only a few settings. Objective: To explore the plasma virome of persons who inject drugs and to characterize the sensitivity and accuracy of NGMS compared with quantitative clinical standards. Design: Longitudinal and cross-sectional studies. Setting: A clinical trial (ClinicalTrials.gov: NCT01285050) and a well-characterized cohort study of persons who have injected drugs. Participants: Persons co-infected with hepatitis C virus (HCV) and HIV. Measurements: Viral nucleic acid in plasma by NGMS and quantitative polymerase chain reaction (PCR). Results: Next-generation metagenomic sequencing generated a total of 600 million reads, which included the expected HIV and HCV RNA sequences. HIV and HCV reads were consistently identified only when samples contained more than 10 000 copies/mL or IU/mL, respectively, as determined by quantitative PCR. A novel RNA virus, human hepegivirus-1 (HHpgV-1), was also detected by NGMS in 4 samples from 2 persons in the clinical trial. Through use of a quantitative PCR assay for HHpgV-1, infection was also detected in 17 (10.9%) of 156 members of a cohort of persons who injected drugs. In these persons, HHpgV-1 viremia persisted for a median of at least 4538 days and was associated with detection of other bloodborne viruses, such as HCV RNA and SEN virus D. Limitation: The medical importance of HHpgV-1 infection is unknown. Conclusion: Although NGMS is insensitive for detection of viruses with relatively low plasma nucleic acid concentrations, it may have broad potential for discovery of new viral infections of possible medical importance, such as HHpgV-1. Primary Funding Source: National Institutes of Health. [Mh] Termos MeSH primário: Infecções por HIV/virologia Hepatite C/virologia Hepevirus/isolamento & purificação Abuso de Substâncias por Via Intravenosa/virologia Viremia/diagnóstico [Mh] Termos MeSH secundário: Coinfecção Estudos Transversais Feminino Biblioteca Genômica Infecções por HIV/complicações Hepatite C/complicações Hepevirus/genética Seres Humanos Estudos Longitudinais Masculino Reação em Cadeia da Polimerase Análise de Sequência de DNA Análise de Sequência de RNA [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170828 [Lr] Data última revisão: 170828 [Sb] Subgrupo de revista: AIM; IM [Da] Data de entrada para processamento: 170607 [St] Status: MEDLINE [do] DOI: 10.7326/M17-0085

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[PMID]: 28222808 [Au] Autor: Wang B; Yang XL; Li W; Zhu Y; Ge XY; Zhang LB; Zhang YZ; Bock CT; Shi ZL [Ad] Endereço: CAS Key Laboratory of Special Pathogens and Biosafety and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. [Ti] Título: Detection and genome characterization of four novel bat hepadnaviruses and a hepevirus in China. [So] Source: Virol J;14(1):40, 2017 Feb 22. [Is] ISSN: 1743-422X [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: In recent years, novel hepadnaviruses, hepeviruses, hepatoviruses, and hepaciviruses have been discovered in various species of bat around the world, indicating that bats may act as natural reservoirs for these hepatitis viruses. In order to further assess the distribution of hepatitis viruses in bat populations in China, we tested the presence of these hepatitis viruses in our archived bat liver samples that originated from several bat species and various geographical regions in China. METHODS: A total of 78 bat liver samples (involving two families, five genera, and 17 species of bat) were examined using nested or heminested reverse transcription PCR (RT-PCR) with degenerate primers. Full-length genomic sequences of two virus strains were sequenced followed by phylogenetic analyses. RESULTS: Four samples were positive for hepadnavirus, only one was positive for hepevirus, and none of the samples were positive for hepatovirus or hepacivirus. The hepadnaviruses were discovered in the horseshoe bats, Rhinolophus sinicus and Rhinolophus affinis, and the hepevirus was found in the whiskered bat Myotis davidii. The full-length genomic sequences were determined for one of the two hepadnaviruses identified in R. sinicus (designated BtHBVRs3364) and the hepevirus (designated BtHEVMd2350). A sequence identity analysis indicated that BtHBVRs3364 had the highest degree of identity with a previously reported hepadnavirus from the roundleaf bat, Hipposideros pomona, from China, and BtHEVMd2350 had the highest degree of identity with a hepevirus found in the serotine bat, Eptesicus serotinus, from Germany, but it exhibited high levels of divergence at both the nucleotide and the amino acid levels. CONCLUSIONS: This is the first study to report that the Chinese horseshoe bat and the Chinese whiskered bat have been found to carry novel hepadnaviruses and a novel hepevirus, respectively. The discovery of BtHBVRs3364 further supports the significance of host switches evolution while opposing the co-evolutionary theory associated with hepadnaviruses. According to the latest criterion of the International Committee on Taxonomy of Viruses (ICTV), we hypothesize that BtHEVMd2350 represents an independent genotype within the species Orthohepevirus D of the family Hepeviridae. [Mh] Termos MeSH primário: Quirópteros/virologia Hepadnaviridae/classificação Hepadnaviridae/isolamento & purificação Hepevirus/classificação Hepevirus/isolamento & purificação Fígado/virologia Filogenia [Mh] Termos MeSH secundário: Animais China Análise por Conglomerados Genoma Viral Hepadnaviridae/genética Hepevirus/genética Reação em Cadeia da Polimerase Reação em Cadeia da Polimerase Via Transcriptase Reversa Análise de Sequência de DNA [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170823 [Lr] Data última revisão: 170823 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170223 [St] Status: MEDLINE [do] DOI: 10.1186/s12985-017-0706-8

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[PMID]: 27876615 [Au] Autor: Reuter G; Boros Á; Mátics R; Kapusinszky B; Delwart E; Pankovics P [Ad] Endereço: Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Pécs, Hungary; Department of Medical Microbiology and Immunology, Medical Center, University of Pécs, Pécs, Hungary. Electronic address: reuter.gabor@gmai [Ti] Título: A novel avian-like hepatitis E virus in wild aquatic bird, little egret (Egretta garzetta), in Hungary. [So] Source: Infect Genet Evol;46:74-77, 2016 Dec. [Is] ISSN: 1567-7257 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Hepatitis E virus (HEV), family Hepeviridae, has public health concerns because of its zoonotic potential; however, the host species spectrum, animal to animal transmissions, the natural chain of hepevirus infections and the genetic diversity of HEV in wildlife especially in birds are less known. Using random amplification and next generation sequencing technology a genetically divergent avian HEV was serendipitously identified in wild bird in Hungary. HEV RNA was detected with high faecal viral load (1.33×10 genomiccopies/ml) measured by real-time PCR in faecal sample from a little egret (Egretta garzetta). The complete genome of HEV strain little egret/kocsag02/2014/HUN (KX589065) is 6660-nt long including a 18-nt 5' end and a 103-nt 3' end (excluding the poly(A)-tail). Sequence analyses indicated that the ORF1 (4554nt/1517aa), ORF2 (1728nt/593aa) and ORF3 (339nt/112aa) encoded proteins of little egret/kocsag02/2014/HUN shared the highest identity (62.8%, 71% and 61.5%) to the corresponding proteins of genotype 1 avian (chicken) HEV in species Orthohepevirus B, respectively. This study reports the identification and complete genome characterization of a novel orthohepevirus distantly related to avian (chicken) HEVs at the first time in wild bird. It is important to recognize all potential hosts, reservoirs and spreaders in nature and to reconstruct the phylogenetic history of hepeviruses. Birds could be an important reservoir of HEV generally and could be infected with genetically highly divergent strains of HEV. [Mh] Termos MeSH primário: Aves/virologia Hepatite Viral Animal/virologia Hepevirus/genética Infecções por Vírus de RNA/virologia [Mh] Termos MeSH secundário: Animais Cloaca/virologia Genoma Viral/genética Hepevirus/classificação Hungria Filogenia RNA Viral/análise RNA Viral/genética Análise de Sequência de RNA Esplenomegalia/virologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (RNA, Viral) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171010 [Lr] Data última revisão: 171010 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161124 [St] Status: MEDLINE

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[PMID]: 27876045 [Au] Autor: Sun Y; Du T; Liu B; Syed SF; Chen Y; Li H; Wang X; Zhang G; Zhou EM; Zhao Q [Ad] Endereço: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China. [Ti] Título: Seroprevalence of avian hepatitis E virus and avian leucosis virus subgroup J in chicken flocks with hepatitis syndrome, China. [So] Source: BMC Vet Res;12(1):261, 2016 Nov 22. [Is] ISSN: 1746-6148 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: From 2014 to 2015 in China, many broiler breeder and layer hen flocks exhibited a decrease in egg production and some chickens developed hepatitis syndrome including hepatomegaly, hepatic necrosis and hemorrhage. Avian hepatitis E virus (HEV) and avian leucosis virus subgroup J (ALV-J) both cause decreasing in egg production, hepatomegaly and hepatic hemorrhage in broiler breeder and layer hens. In the study, the seroprevalence of avian HEV and ALV-J in these flocks emerging the disease from Shandong and Shaanxi provinces were investigated. RESULTS: A total of 1995 serum samples were collected from 14 flocks with hepatitis syndrome in Shandong and Shaanxi provinces, China. Antibodies against avian HEV and ALV-J in these serum samples were detected using iELISAs. The seroprevalence of anti-avian HEV antibodies (35.09%) was significantly higher than that of anti-ALV-J antibodies (2.16%) (p = 0.00). Moreover, the 43 serum samples positive for anti-ALV-J antibodies were all also positive for anti-avian HEV antibodies. In a comparison of both provinces, Shandong chickens exhibited a significantly higher seroprevalence of anti-avian HEV antibodies (42.16%) than Shaanxi chickens (26%) (p = 0.00). In addition, the detection of avian HEV RNA and ALV-J cDNA in the liver samples from the flocks of two provinces also showed the same results of the seroprevalence. CONCLUSIONS: In the present study, the results showed that avian HEV infection is widely prevalent and ALV-J infection is endemic in the flocks with hepatitis syndrome from Shandong and Shaanxi provinces of China. These results suggested that avian HEV infection may be the major cause of increased egg drop and hepatitis syndrome observed during the last 2 years in China. These results should be useful to guide development of prevention and control measures to control the diseases within chicken flocks in China. [Mh] Termos MeSH primário: Leucose Aviária/epidemiologia Hepatite E/veterinária Doenças das Aves Domésticas/epidemiologia [Mh] Termos MeSH secundário: Animais Anticorpos Antivirais/sangue Leucose Aviária/patologia Vírus da Leucose Aviária/genética Vírus da Leucose Aviária/fisiologia Galinhas China/epidemiologia DNA Complementar/análise Ensaio de Imunoadsorção Enzimática/veterinária Hepatite E/epidemiologia Hepevirus/genética Hepevirus/fisiologia Fígado/virologia Doenças das Aves Domésticas/patologia RNA Viral/análise Estudos Soroepidemiológicos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antibodies, Viral); 0 (DNA, Complementary); 0 (RNA, Viral) [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 170220 [Lr] Data última revisão: 170220 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161124 [St] Status: MEDLINE

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[PMID]: 27610734 [Au] Autor: Yang S; Wang L; Sun S [Ad] Endereço: Shandong Agricultural University, Tai An, Shandong Province, 271000, People's Republic of China. [Ti] Título: Natural Infection with Avian Hepatitis E Virus and Marek's Disease Virus in Brown Layer Chickens in China. [So] Source: Avian Dis;60(3):698-704, 2016 Sep. [Is] ISSN: 1938-4351 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: In the present study, avian hepatitis E virus (HEV) and serotype-1 strains of Marek's disease virus (MDV-1) were detected from a flock of 27-wk-old brown layer hens in China, accompanied by an average daily mortality of 0.44%. Postmortem examination of 25 sick hens and five apparently healthy hens selected randomly from the flock showed significant pathologic changes consistent with hepatitis-splenomegaly syndrome (HSS), including hepatomegaly, peritoneal fluid, and hepatic subcapsular hemorrhages. Microscopic examination of these livers showed multifocal necrotizing hepatitis and mild lymphocytic infiltration. These liver samples were investigated for HEV by reverse-transcription PCR. The overall detection rate of HEV RNA in samples of sick chickens was about 56% (14/25), while in samples from apparently healthy hens, it was 80% (4/5). Sequencing analysis of three 242-base-pair fragments of the helicase gene revealed 95.5% to 97.9% nucleotide identity compared with published avian HEV genotype 3, whereas identities demonstrated only 77.3% to 86.0% similarity when compared with genotypes 1, 2, and 4. Unexpectedly, the MDV meq gene was detected in livers from both apparently healthy chickens (2/5) and sick chickens (12/25) by PCR analysis. The meq gene (396 base pairs) was determined to belong to MDV-1 by further sequencing. The co-infection rate of avian HEV and MDV in this flock was 30% (9/30). This is the first report of dual infection of a nonenvelope RNA virus (HEV) with a herpesvirus (MDV) in chickens in China. [Mh] Termos MeSH primário: Galinhas Coinfecção/veterinária Doenças das Aves Domésticas/epidemiologia [Mh] Termos MeSH secundário: Animais Sequência de Bases China/epidemiologia Coinfecção/epidemiologia Coinfecção/virologia Feminino Hepatite Viral Animal/epidemiologia Hepatite Viral Animal/virologia Hepevirus/isolamento & purificação Herpesvirus Galináceo 2/isolamento & purificação Doença de Marek/epidemiologia Doença de Marek/virologia Doenças das Aves Domésticas/virologia Infecções por Vírus de RNA/epidemiologia Infecções por Vírus de RNA/veterinária Infecções por Vírus de RNA/virologia Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária [Pt] Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170901 [Lr] Data última revisão: 170901 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160910 [St] Status: MEDLINE [do] DOI: 10.1637/11386-013016-Reg.1

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[PMID]: 27164843 [Au] Autor: Moon HW; Lee BW; Sung HW; Yoon BI; Kwon HM [Ad] Endereço: College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon, Gangwon, 200-701, Republic of Korea. [Ti] Título: Identification and characterization of avian hepatitis E virus genotype 2 from chickens with hepatitis-splenomegaly syndrome in Korea. [So] Source: Virus Genes;52(5):738-42, 2016 Oct. [Is] ISSN: 1572-994X [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: A new avian hepatitis E virus (HEV) GI-B was identified in broiler breeders with hematomas, liver rupture, and splenomegaly, along with excessive abdominal fat, in Korea. Previously, genotype 1 had been identified in avian HEV strains in Korea. Complete sequence analyses revealed that the new avian HEV clustered in genotype 2, which has been identified in the USA and Spain; the GI-B isolate was closely related to the USA prototype avian HEV isolated from a chicken with hepatitis-splenomegaly syndrome. Although some HEV genotypes show a geographical distribution pattern, the discovery of genotype 2 in addition to genotype 1 in Korea suggests that the geographical grouping might be reconsidered. These findings have important implications for understanding the global epidemiology and spread of avian HEV. [Mh] Termos MeSH primário: Galinhas/virologia Hepatite E/virologia Hepatite Viral Animal/virologia Hepevirus/genética Doenças das Aves Domésticas/virologia Esplenomegalia/virologia [Mh] Termos MeSH secundário: Sequência de Aminoácidos Animais Genótipo Filogenia República da Coreia Espanha [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 171102 [Lr] Data última revisão: 171102 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160512 [St] Status: MEDLINE [do] DOI: 10.1007/s11262-016-1351-9

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[PMID]: 27073893 [Au] Autor: Zhang X; Bilic I; Marek A; Glösmann M; Hess M [Ad] Endereço: Clinic for Poultry and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria. [Ti] Título: C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells. [So] Source: PLoS One;11(4):e0153723, 2016. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The infection of chickens with avian Hepatitis E virus (avian HEV) can be asymptomatic or induces clinical signs characterized by increased mortality and decreased egg production in adult birds. Due to the lack of an efficient cell culture system for avian HEV, the interaction between virus and host cells is still barely understood. In this study, four truncated avian HEV capsid proteins (ORF2-1 - ORF2-4) with an identical 338aa deletion at the N-terminus and gradual deletions from 0, 42, 99 and 136aa at the C-terminus, respectively, were expressed and used to map the possible binding site within avian HEV capsid protein. Results from the binding assay showed that three truncated capsid proteins attached to avian LMH cells, but did not penetrate into cells. However, the shortest construct, ORF2-4, lost the capability of binding to cells suggesting that the presence of amino acids 471 to 507 of the capsid protein is crucial for the attachment. The construct ORF2-3 (aa339-507) was used to study the potential binding of avian HEV capsid protein to human and other avian species. It could be demonstrated that ORF2-3 was capable of binding to QT-35 cells from Japanese quail and human HepG2 cells but failed to bind to P815 cells. Additionally, chicken serum raised against ORF2-3 successfully blocked the binding to LMH cells. Treatment with heparin sodium salt or sodium chlorate significantly reduced binding of ORF2-3 to LMH cells. However, heparinase II treatment of LMH cells had no effect on binding of the ORF2-3 construct, suggesting a possible distinct attachment mechanism of avian as compared to human HEV. For the first time, interactions between avian HEV capsid protein and host cells were investigated demonstrating that aa471 to 507 of the capsid protein are needed to facilitate interaction with different kind of cells from different species. [Mh] Termos MeSH primário: Proteínas do Capsídeo/metabolismo Hepatite Viral Animal/metabolismo Hepevirus/metabolismo Infecções por Vírus de RNA/metabolismo [Mh] Termos MeSH secundário: Animais Galinhas/virologia Hepatite Viral Animal/virologia Interações Hospedeiro-Patógeno Seres Humanos Infecções por Vírus de RNA/virologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Capsid Proteins) [Em] Mês de entrada: 1608 [Cu] Atualização por classe: 160423 [Lr] Data última revisão: 160423 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160414 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0153723

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[PMID]: 26605326 [Au] Autor: Li H; Zhu R; She R; Zhang C; Shi R; Li W; Du F; Wu Q; Hu F; Zhang Y; Soomro MH; Zheng C [Ad] Endereço: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. [Ti] Título: Case Report Associated with Aspergillosis and Hepatitis E Virus Coinfection in Himalayan Griffons. [So] Source: Biomed Res Int;2015:287315, 2015. [Is] ISSN: 2314-6141 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: This study involved a death which occurred in four Himalayan griffons housed in Beijing zoo, China. Based on pathogen identification and the pathological changes observed, we did characterize the fungi and Hepatitis E virus (HEV) in four dead Himalayan griffons. Pathological changes were severe. Membranous-like material was observed on the surface of the internal organs. Spleen was necrotic. Focal lymphocyte infiltration in the liver and many sunflower-like fungi nodules were evident in the tissues, especially in the kidney. PCR was used to identify the pathogen. Based on the 18SrRNA genomic sequence of known fungi, the results confirmed that all four dead Himalayan griffons were infected with Aspergillus. At the same time the detection of HEV also showed positive results. To the best of our knowledge, this work appears to be the first report of concurrent presence of Aspergillosis and Hepatitis E virus in rare avian species. [Mh] Termos MeSH primário: Aspergilose Coinfecção Falconiformes Hepatite E Hepevirus [Mh] Termos MeSH secundário: Animais Aspergilose/veterinária Aspergilose/virologia Doenças das Aves/microbiologia Doenças das Aves/virologia China Hepatite E/microbiologia Hepatite E/veterinária [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Em] Mês de entrada: 1609 [Cu] Atualização por classe: 170220 [Lr] Data última revisão: 170220 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 151126 [St] Status: MEDLINE [do] DOI: 10.1155/2015/287315

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2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

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