Base de dados : MEDLINE
Pesquisa : B04.820.455 [Categoria DeCS]
Referências encontradas : 43 [refinar]
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  1 / 43 MEDLINE  
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[PMID]:28389807
[Au] Autor:Amarasinghe GK; Bào Y; Basler CF; Bavari S; Beer M; Bejerman N; Blasdell KR; Bochnowski A; Briese T; Bukreyev A; Calisher CH; Chandran K; Collins PL; Dietzgen RG; Dolnik O; Dürrwald R; Dye JM; Easton AJ; Ebihara H; Fang Q; Formenty P; Fouchier RAM; Ghedin E; Harding RM; Hewson R; Higgins CM; Hong J; Horie M; James AP; Jiang D; Kobinger GP; Kondo H; Kurath G; Lamb RA; Lee B; Leroy EM; Li M; Maisner A; Mühlberger E; Netesov SV; Nowotny N; Patterson JL; Payne SL; Paweska JT; Pearson MN; Randall RE; Revill PA; Rima BK; Rota P; Rubbenstroth D
[Ad] Endereço:Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA.
[Ti] Título:Taxonomy of the order Mononegavirales: update 2017.
[So] Source:Arch Virol;162(8):2493-2504, 2017 Aug.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:In 2017, the order Mononegavirales was expanded by the inclusion of a total of 69 novel species. Five new rhabdovirus genera and one new nyamivirus genus were established to harbor 41 of these species, whereas the remaining new species were assigned to already established genera. Furthermore, non-Latinized binomial species names replaced all paramyxovirus and pneumovirus species names, thereby accomplishing application of binomial species names throughout the entire order. This article presents the updated taxonomy of the order Mononegavirales as now accepted by the International Committee on Taxonomy of Viruses (ICTV).
[Mh] Termos MeSH primário: Genoma Viral
Mononegavirais/classificação
[Mh] Termos MeSH secundário: Ordem dos Genes
Mononegavirais/genética
Filogenia
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170409
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3311-7


  2 / 43 MEDLINE  
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[PMID]:27487481
[Au] Autor:Chatterjee S; Basler CF; Amarasinghe GK; Leung DW
[Ad] Endereço:Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
[Ti] Título:Molecular Mechanisms of Innate Immune Inhibition by Non-Segmented Negative-Sense RNA Viruses.
[So] Source:J Mol Biol;428(17):3467-82, 2016 Aug 28.
[Is] ISSN:1089-8638
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The host innate immune system serves as the first line of defense against viral infections. Germline-encoded pattern recognition receptors detect molecular patterns associated with pathogens and activate innate immune responses. Of particular relevance to viral infections are those pattern recognition receptors that activate type I interferon responses, which establish an antiviral state. The order Mononegavirales is composed of viruses that possess single-stranded, non-segmented negative-sense (NNS) RNA genomes and are important human pathogens that consistently antagonize signaling related to type I interferon responses. NNS viruses have limited encoding capacity compared to many DNA viruses, and as a likely consequence, most open reading frames encode multifunctional viral proteins that interact with host factors in order to evade host cell defenses while promoting viral replication. In this review, we will discuss the molecular mechanisms of innate immune evasion by select NNS viruses. A greater understanding of these interactions will be critical in facilitating the development of effective therapeutics and viral countermeasures.
[Mh] Termos MeSH primário: Interações Hospedeiro-Patógeno
Evasão da Resposta Imune
Tolerância Imunológica
Imunidade Inata
Mononegavirais/imunologia
Mononegavirais/patogenicidade
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Modelos Biológicos
Modelos Moleculares
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160804
[St] Status:MEDLINE


  3 / 43 MEDLINE  
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[PMID]:27216929
[Au] Autor:Afonso CL; Amarasinghe GK; Bányai K; Bào Y; Basler CF; Bavari S; Bejerman N; Blasdell KR; Briand FX; Briese T; Bukreyev A; Calisher CH; Chandran K; Chéng J; Clawson AN; Collins PL; Dietzgen RG; Dolnik O; Domier LL; Dürrwald R; Dye JM; Easton AJ; Ebihara H; Farkas SL; Freitas-Astúa J; Formenty P; Fouchier RA; Fù Y; Ghedin E; Goodin MM; Hewson R; Horie M; Hyndman TH; Jiang D; Kitajima EW; Kobinger GP; Kondo H; Kurath G; Lamb RA; Lenardon S; Leroy EM; Li CX; Lin XD; Liú L; Longdon B; Marton S; Maisner A; Mühlberger E; Netesov SV; Nowotny N
[Ad] Endereço:Southeast Poultry Research Laboratory, Agricultural Research Service, US Department of Agriculture, Athens, GA, USA.
[Ti] Título:Taxonomy of the order Mononegavirales: update 2016.
[So] Source:Arch Virol;161(8):2351-60, 2016 Aug.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:In 2016, the order Mononegavirales was emended through the addition of two new families (Mymonaviridae and Sunviridae), the elevation of the paramyxoviral subfamily Pneumovirinae to family status (Pneumoviridae), the addition of five free-floating genera (Anphevirus, Arlivirus, Chengtivirus, Crustavirus, and Wastrivirus), and several other changes at the genus and species levels. This article presents the updated taxonomy of the order Mononegavirales as now accepted by the International Committee on Taxonomy of Viruses (ICTV).
[Mh] Termos MeSH primário: Genoma Viral
Mononegavirais/classificação
[Mh] Termos MeSH secundário: Mononegavirais/genética
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160525
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-016-2880-1


  4 / 43 MEDLINE  
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[PMID]:26498797
[Au] Autor:Gotesman M; Menanteau-Ledouble S; El-Matbouli M
[Ad] Endereço:Department of Biology, Technion - Israel Institute of Technology, Technion, Haifa, Israel.
[Ti] Título:Proteomic Analysis of Cytoskeleton Proteins in Fish.
[So] Source:Methods Mol Biol;1365:357-72, 2016.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this chapter, we describe laboratory protocols for rearing fish and a simple and efficient method of extracting and identifying pathogen and host proteins that may be involved in entry and replication of commercially important fish viruses. We have used the common carp (Cyprinus carpio L.) and goldfish (Cyprinus auratus) as a model system for studies of proteins involved in viral entry and replication. The chapter describes detailed protocols for maintenance of carp, cell culture, antibody purification of proteins, and use of electrospray-ionization mass spectrometry analysis to screen and identify cytoskeleton and other proteins that may be involved in viral infection and propagation in fish.
[Mh] Termos MeSH primário: Carpas/metabolismo
Proteínas do Citoesqueleto/metabolismo
Proteínas de Peixes/metabolismo
Carpa Dourada/metabolismo
Proteômica/métodos
[Mh] Termos MeSH secundário: Alphaherpesvirinae/fisiologia
Animais
Anticorpos Monoclonais/imunologia
Carpas/virologia
Células Cultivadas
Proteínas do Citoesqueleto/imunologia
Proteínas de Peixes/imunologia
Carpa Dourada/virologia
Injeções
Mononegavirais/fisiologia
Internalização do Vírus
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Cytoskeletal Proteins); 0 (Fish Proteins)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151027
[Lr] Data última revisão:
151027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151027
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-3124-8_21


  5 / 43 MEDLINE  
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[PMID]:26549102
[Au] Autor:Paesen GC; Collet A; Sallamand C; Debart F; Vasseur JJ; Canard B; Decroly E; Grimes JM
[Ad] Endereço:Division of Structural Biology, Wellcome Trust Centre for Human Genetics, Oxford OX3 7BN, UK.
[Ti] Título:X-ray structure and activities of an essential Mononegavirales L-protein domain.
[So] Source:Nat Commun;6:8749, 2015 Nov 09.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The L protein of mononegaviruses harbours all catalytic activities for genome replication and transcription. It contains six conserved domains (CR-I to -VI; Fig. 1a). CR-III has been linked to polymerase and polyadenylation activity, CR-V to mRNA capping and CR-VI to cap methylation. However, how these activities are choreographed is poorly understood. Here we present the 2.2-Å X-ray structure and activities of CR-VI+, a portion of human Metapneumovirus L consisting of CR-VI and the poorly conserved region at its C terminus, the +domain. The CR-VI domain has a methyltransferase fold, which besides the typical S-adenosylmethionine-binding site ((SAM)P) also contains a novel pocket ((NS)P) that can accommodate a nucleoside. CR-VI lacks an obvious cap-binding site, and the (SAM)P-adjoining site holding the nucleotides undergoing methylation ((SUB)P) is unusually narrow because of the overhanging +domain. CR-VI+ sequentially methylates caps at their 2'O and N7 positions, and also displays nucleotide triphosphatase activity.
[Mh] Termos MeSH primário: Metapneumovirus/metabolismo
Capuzes de RNA/metabolismo
RNA/metabolismo
S-Adenosilmetionina/metabolismo
[Mh] Termos MeSH secundário: Animais
Sítios de Ligação
Cromatografia em Camada Delgada
Cristalização
Cristalografia por Raios X
Metilação
Mononegavirais/metabolismo
Estrutura Terciária de Proteína
RNA Replicase/química
RNA Replicase/metabolismo
Células Sf9
Spodoptera
Proteínas Virais/química
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA Caps); 0 (Viral Proteins); 63231-63-0 (RNA); 7LP2MPO46S (S-Adenosylmethionine); EC 2.7.7.48 (RNA Replicase)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151110
[St] Status:MEDLINE
[do] DOI:10.1038/ncomms9749


  6 / 43 MEDLINE  
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[PMID]:25702088
[Au] Autor:Pfaller CK; Cattaneo R; Schnell MJ
[Ad] Endereço:Department of Molecular Medicine, Mayo Clinic, Rochester, MN 55905, USA.
[Ti] Título:Reverse genetics of Mononegavirales: How they work, new vaccines, and new cancer therapeutics.
[So] Source:Virology;479-480:331-44, 2015 May.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The order Mononegavirales includes five families: Bornaviridae, Filoviridae, Nyamaviridae, Paramyxoviridae, and Rhabdoviridae. The genome of these viruses is one molecule of negative-sense single strand RNA coding for five to ten genes in a conserved order. The RNA is not infectious until packaged by the nucleocapsid protein and transcribed by the polymerase and co-factors. Reverse genetics approaches have answered fundamental questions about the biology of Mononegavirales. The lack of icosahedral symmetry and modular organization in the genome of these viruses has facilitated engineering of viruses expressing fluorescent proteins, and these fluorescent proteins have provided important insights about the molecular and cellular basis of tissue tropism and pathogenesis. Studies have assessed the relevance for virulence of different receptors and the interactions with cellular proteins governing the innate immune responses. Research has also analyzed the mechanisms of attenuation. Based on these findings, ongoing clinical trials are exploring new live attenuated vaccines and the use of viruses re-engineered as cancer therapeutics.
[Mh] Termos MeSH primário: Mononegavirais/genética
Mononegavirais/fisiologia
Genética Reversa
[Mh] Termos MeSH secundário: Interações Hospedeiro-Patógeno
Seres Humanos
Mononegavirais/patogenicidade
Neoplasias/terapia
Terapia Viral Oncolítica/métodos
Vacinas Atenuadas/imunologia
Vacinas Atenuadas/isolamento & purificação
Tropismo Viral
Vacinas Virais/imunologia
Vacinas Virais/isolamento & purificação
Montagem de Vírus
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; REVIEW
[Nm] Nome de substância:
0 (Vaccines, Attenuated); 0 (Viral Vaccines)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150223
[St] Status:MEDLINE


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[PMID]:25056893
[Au] Autor:Tokarz R; Williams SH; Sameroff S; Sanchez Leon M; Jain K; Lipkin WI
[Ad] Endereço:Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, USA rt2249@cumc.columbia.edu.
[Ti] Título:Virome analysis of Amblyomma americanum, Dermacentor variabilis, and Ixodes scapularis ticks reveals novel highly divergent vertebrate and invertebrate viruses.
[So] Source:J Virol;88(19):11480-92, 2014 Oct.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: A wide range of bacterial pathogens have been identified in ticks, yet the diversity of viruses in ticks is largely unexplored. In the United States, Amblyomma americanum, Dermacentor variabilis, and Ixodes scapularis are among the principal tick species associated with pathogen transmission. We used high-throughput sequencing to characterize the viromes of these tick species and identified the presence of Powassan virus and eight novel viruses. These included the most divergent nairovirus described to date, two new clades of tick-borne phleboviruses, a mononegavirus, and viruses with similarity to plant and insect viruses. Our analysis revealed that ticks are reservoirs for a wide range of viruses and suggests that discovery and characterization of tick-borne viruses will have implications for viral taxonomy and may provide insight into tick-transmitted diseases. IMPORTANCE: Ticks are implicated as vectors of a wide array of human and animal pathogens. To better understand the extent of tick-borne diseases, it is crucial to uncover the full range of microbial agents associated with ticks. Our current knowledge of the diversity of tick-associated viruses is limited, in part due to the lack of investigation of tick viromes. In this study, we examined the viromes of three tick species from the United States. We found that ticks are hosts to highly divergent viruses across several taxa, including ones previously associated with human disease. Our data underscore the diversity of tick-associated viruses and provide the foundation for further studies into viral etiology of tick-borne diseases.
[Mh] Termos MeSH primário: Vetores Aracnídeos
RNA Polimerases Dirigidas por DNA/genética
Genoma Viral
Filogenia
Carrapatos
Proteínas Virais/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Dermacentor/classificação
Dermacentor/genética
Reservatórios de Doenças
Vírus da Encefalite Transmitidos por Carrapatos/classificação
Vírus da Encefalite Transmitidos por Carrapatos/genética
Vírus da Encefalite Transmitidos por Carrapatos/isolamento & purificação
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Ixodes/classificação
Ixodes/genética
Dados de Sequência Molecular
Mononegavirais/classificação
Mononegavirais/genética
Mononegavirais/isolamento & purificação
Nairovirus/classificação
Nairovirus/genética
Nairovirus/isolamento & purificação
Phlebovirus/classificação
Phlebovirus/genética
Phlebovirus/isolamento & purificação
Alinhamento de Sequência
Infestações por Carrapato/epidemiologia
Infestações por Carrapato/virologia
Carrapatos/classificação
Carrapatos/genética
Estados Unidos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Viral Proteins); EC 2.7.7.6 (DNA-Directed RNA Polymerases)
[Em] Mês de entrada:1411
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140725
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.01858-14


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[PMID]:24778199
[Au] Autor:Rogers MB; Cui L; Fitch A; Popov V; Travassos da Rosa AP; Vasilakis N; Tesh RB; Ghedin E
[Ad] Endereço:Center for Vaccine Research, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania; Department of Computational and Systems Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania; Tsinghua University School of Medicine, Beijing, China; Center for Biodefense an
[Ti] Título:Whole genome analysis of sierra nevada virus, a novel mononegavirus in the family nyamiviridae.
[So] Source:Am J Trop Med Hyg;91(1):159-64, 2014 Jul.
[Is] ISSN:1476-1645
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A novel mononegavirus was isolated in 1975 from ticks (Ornithodoros coriaceus) collected during investigation of an outbreak of epizootic bovine abortion (EBA) in northern California. It was originally designated "bovine abortion-tick virus" (BA-T virus). The EBA is now known to be associated with a deltaproteobacterium infection, and not a virus. The BA-T virus had remained uncharacterized until now. We have determined by electron microscopy, serology, and genome sequencing that the BA-T virus is a fourth member of the newly proposed family Nyamiviridae, and we have renamed it Sierra Nevada virus (SNVV). Although antigenically distinct, phylogenetically SNVV is basal to Nyamanini virus (NYMV) and Midway virus (MIDWV), two other tick-borne agents. Although NYMV was found to infect land birds, and MIDWV seabirds, it is presently unknown whether SNVV naturally infects birds or mammals.
[Mh] Termos MeSH primário: Doenças dos Bovinos/virologia
Genoma Viral
Infecções por Mononegavirales/veterinária
Mononegavirais/classificação
Mononegavirais/genética
Filogenia
[Mh] Termos MeSH secundário: Aborto Animal/virologia
Sequência de Aminoácidos
Animais
Vetores Aracnídeos/virologia
Sequência de Bases
Bovinos
Cercopithecus aethiops
Mapeamento Cromossômico
Feminino
Camundongos
Microscopia Eletrônica de Transmissão
Anotação de Sequência Molecular
Mononegavirais/isolamento & purificação
Infecções por Mononegavirales/virologia
Ornithodoros/virologia
Alinhamento de Sequência
Análise de Sequência de DNA
Homologia de Sequência de Aminoácidos
Células Vero
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1409
[Cu] Atualização por classe:161203
[Lr] Data última revisão:
161203
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:140430
[St] Status:MEDLINE
[do] DOI:10.4269/ajtmh.14-0076


  9 / 43 MEDLINE  
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[PMID]:24462694
[Au] Autor:Falzarano D; Groseth A; Hoenen T
[Ad] Endereço:Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.
[Ti] Título:Development and application of reporter-expressing mononegaviruses: current challenges and perspectives.
[So] Source:Antiviral Res;103:78-87, 2014 Mar.
[Is] ISSN:1872-9096
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Reverse genetics allows the generation of recombinant viruses entirely from cDNA. One application of this technology is the creation of reporter-expressing viruses, which greatly increase the detail and ease with which these viruses can be studied. However, there are a number of challenges when working with reporter-expressing viruses. Both the reporter protein itself as well as the genetic manipulations within the viral genome required for expression of this reporter can result in altered biological properties of the recombinant virus, and lead to attenuation in vitro and/or in vivo. Further, instability of reporter expression and purging of the genetic information encoding for the reporter from the viral genome can be an issue. Finally, a practical challenge for in vivo studies lies in the attenuation of light signals when traversing tissues. Novel expression strategies and the continued development of brighter, red and far-red shifted reporters and the increased use of bioluminescent reporters for in vivo applications promise to overcome some of these limitations in future. However, a "one size fits all" approach to the design of reporter-expressing viruses has thus far not been possible. Rather, a reporter suited to the intended application must be selected and an appropriate expression strategy and location for the reporter in the viral genome chosen. Still, attenuating effects of the reporter on viral fitness are difficult to predict and have to be carefully assessed with respect to the intended application. Despite these limitations the generation of suitable reporter-expressing viruses will become more common as technology and our understanding of the intricacies of viral gene expression and regulation improves, allowing deeper insight into virus biology both in living cells and in animals.
[Mh] Termos MeSH primário: Expressão Gênica
Genes Reporter
Mononegavirais/fisiologia
Genética Reversa/métodos
Coloração e Rotulagem/métodos
Virologia/métodos
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Mononegavirais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., INTRAMURAL; REVIEW
[Em] Mês de entrada:1410
[Cu] Atualização por classe:161025
[Lr] Data última revisão:
161025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140128
[St] Status:MEDLINE


  10 / 43 MEDLINE  
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[PMID]:24069485
[Au] Autor:Koehler JW; Smith JM; Ripoll DR; Spik KW; Taylor SL; Badger CV; Grant RJ; Ogg MM; Wallqvist A; Guttieri MC; Garry RF; Schmaljohn CS
[Ad] Endereço:United States Army Medical Research Institute of Infectious Diseases, Virology Division, Fort Detrick, Maryland, United States of America.
[Ti] Título:A fusion-inhibiting peptide against Rift Valley fever virus inhibits multiple, diverse viruses.
[So] Source:PLoS Negl Trop Dis;7(9):e2430, 2013.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:For enveloped viruses, fusion of the viral envelope with a cellular membrane is critical for a productive infection to occur. This fusion process is mediated by at least three classes of fusion proteins (Class I, II, and III) based on the protein sequence and structure. For Rift Valley fever virus (RVFV), the glycoprotein Gc (Class II fusion protein) mediates this fusion event following entry into the endocytic pathway, allowing the viral genome access to the cell cytoplasm. Here, we show that peptides analogous to the RVFV Gc stem region inhibited RVFV infectivity in cell culture by inhibiting the fusion process. Further, we show that infectivity can be inhibited for diverse, unrelated RNA viruses that have Class I (Ebola virus), Class II (Andes virus), or Class III (vesicular stomatitis virus) fusion proteins using this single peptide. Our findings are consistent with an inhibition mechanism similar to that proposed for stem peptide fusion inhibitors of dengue virus in which the RVFV inhibitory peptide first binds to both the virion and cell membranes, allowing it to traffic with the virus into the endocytic pathway. Upon acidification and rearrangement of Gc, the peptide is then able to specifically bind to Gc and prevent fusion of the viral and endocytic membranes, thus inhibiting viral infection. These results could provide novel insights into conserved features among the three classes of viral fusion proteins and offer direction for the future development of broadly active fusion inhibitors.
[Mh] Termos MeSH primário: Antivirais/metabolismo
Bunyaviridae/fisiologia
Mononegavirais/fisiologia
Proteínas Virais de Fusão/metabolismo
Internalização do Vírus
[Mh] Termos MeSH secundário: Animais
Bunyaviridae/efeitos dos fármacos
Cercopithecus aethiops
Ebolavirus/efeitos dos fármacos
Ebolavirus/fisiologia
Mononegavirais/efeitos dos fármacos
Células Vero
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Viral Fusion Proteins)
[Em] Mês de entrada:1403
[Cu] Atualização por classe:150422
[Lr] Data última revisão:
150422
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130927
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0002430



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