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  1 / 119 MEDLINE  
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[PMID]:28185101
[Au] Autor:Garcia-Barrera AA; Del Valle A; Montaño-Hirose JA; Barrón BL; Salinas-Trujano J; Torres-Flores J
[Ad] Endereço:Laboratorio de Virología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala S/N, Casco de Santo Tomás, 11340, Mexico City, Mexico.
[Ti] Título:Full-genome sequencing and phylogenetic analysis of four neurovirulent Mexican isolates of porcine rubulavirus.
[So] Source:Arch Virol;162(6):1765-1768, 2017 Jun.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:We report the complete genome sequences of four neurovirulent isolates of porcine rubulavirus (PorPV) from 2015 and one historical PorPV isolate from 1984 obtained by next-generation sequencing. A phylogenetic tree constructed using the individual sequences of the complete HN genes of the 2015 isolates and other historical sequences deposited in the GenBank database revealed that several recent neurovirulent isolates of PorPV (2008-2015) cluster together in a separate clade. Phylogenetic analysis of the complete genome sequences revealed that the neurovirulent strains of PorPV that circulated in Mexico during 2015 are genetically different from the PorPV strains that circulated during the 1980s.
[Mh] Termos MeSH primário: Genoma Viral
Filogenia
Infecções por Rubulavirus/veterinária
Rubulavirus/isolamento & purificação
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
México
Dados de Sequência Molecular
RNA Viral/genética
Rubulavirus/classificação
Rubulavirus/genética
Infecções por Rubulavirus/virologia
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170630
[Lr] Data última revisão:
170630
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3267-7


  2 / 119 MEDLINE  
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[PMID]:27619056
[Au] Autor:Yadav P; Sarkale P; Patil D; Shete A; Kokate P; Kumar V; Jain R; Jadhav S; Basu A; Pawar S; Sudeep A; Gokhale M; Lakra R; Mourya D
[Ad] Endereço:National Institute of Virology, Pune, 20-A, Dr. Ambedkar Road, Pune, Maharashtra Pin 411001, India.
[Ti] Título:Isolation of Tioman virus from Pteropus giganteus bat in North-East region of India.
[So] Source:Infect Genet Evol;45:224-229, 2016 Nov.
[Is] ISSN:1567-7257
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Bat-borne viral diseases are a major public health concern among newly emerging infectious diseases which includes severe acute respiratory syndrome, Nipah, Marburg and Ebola virus disease. During the survey for Nipah virus among bats at North-East region of India; Tioman virus (TioV), a new member of the Paramyxoviridae family was isolated from tissues of Pteropus giganteus bats for the first time in India. This isolate was identified and confirmed by RT-PCR, sequence analysis and electron microscopy. A range of vertebrate cell lines were shown to be susceptible to Tioman virus. Negative electron microscopy study revealed the "herringbone" morphology of the nucleocapsid filaments and enveloped particles with distinct envelope projections a characteristic of the Paramyxoviridae family. Sequence analysis of Nucleocapsid gene of TioV demonstrated sequence identity of 99.87% and 99.99% nucleotide and amino acid respectively with of TioV strain isolated in Malaysia, 2001. This report demonstrates the first isolation of Tioman virus from a region where Nipah virus activity has been noticed in the past and recent years. Bat-borne viruses have become serious concern world-wide. A Survey of bats for novel viruses in this region would help in recognizing emerging viruses and combating diseases caused by them.
[Mh] Termos MeSH primário: Quirópteros/virologia
Infecções por Rubulavirus
Rubulavirus
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Embrião de Galinha
Índia
Rubulavirus/classificação
Rubulavirus/genética
Rubulavirus/isolamento & purificação
Infecções por Rubulavirus/veterinária
Infecções por Rubulavirus/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160914
[St] Status:MEDLINE


  3 / 119 MEDLINE  
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[PMID]:27512068
[Au] Autor:Young DF; Andrejeva J; Li X; Inesta-Vaquera F; Dong C; Cowling VH; Goodbourn S; Randall RE
[Ad] Endereço:School of Biology, Centre for Biomolecular Sciences, University of St. Andrews, St. Andrews, Fife, United Kingdom.
[Ti] Título:Human IFIT1 Inhibits mRNA Translation of Rubulaviruses but Not Other Members of the Paramyxoviridae Family.
[So] Source:J Virol;90(20):9446-56, 2016 Oct 15.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: We have previously shown that IFIT1 is primarily responsible for the antiviral action of interferon (IFN) alpha/beta against parainfluenza virus type 5 (PIV5), selectively inhibiting the translation of PIV5 mRNAs. Here we report that while PIV2, PIV5, and mumps virus (MuV) are sensitive to IFIT1, nonrubulavirus members of the paramyxoviridae such as PIV3, Sendai virus (SeV), and canine distemper virus (CDV) are resistant. The IFIT1 sensitivity of PIV5 was not rescued by coinfection with an IFIT1-resistant virus (PIV3), demonstrating that PIV3 does not specifically inhibit the antiviral activity of IFIT1 and that the inhibition of PIV5 mRNAs is regulated by cis-acting elements. We developed an in vitro translation system using purified human IFIT1 to further investigate the mechanism of action of IFIT1. While the translations of PIV2, PIV5, and MuV mRNAs were directly inhibited by IFIT1, the translations of PIV3, SeV, and CDV mRNAs were not. Using purified human mRNA-capping enzymes, we show biochemically that efficient inhibition by IFIT1 is dependent upon a 5' guanosine nucleoside cap (which need not be N7 methylated) and that this sensitivity is partly abrogated by 2'O methylation of the cap 1 ribose. Intriguingly, PIV5 M mRNA, in contrast to NP mRNA, remained sensitive to inhibition by IFIT1 following in vitro 2'O methylation, suggesting that other structural features of mRNAs may influence their sensitivity to IFIT1. Thus, surprisingly, the viral polymerases (which have 2'-O-methyltransferase activity) of rubulaviruses do not protect these viruses from inhibition by IFIT1. Possible biological consequences of this are discussed. IMPORTANCE: Paramyxoviruses cause a wide variety of diseases, and yet most of their genes encode structural proteins and proteins involved in their replication cycle. Thus, the amount of genetic information that determines the type of disease that paramyxoviruses cause is relatively small. One factor that will influence disease outcomes is how they interact with innate host cell defenses, including the interferon (IFN) system. Here we show that different paramyxoviruses interact in distinct ways with cells in a preexisting IFN-induced antiviral state. Strikingly, all the rubulaviruses tested were sensitive to the antiviral action of ISG56/IFIT1, while all the other paramyxoviruses tested were resistant. We developed novel in vitro biochemical assays to investigate the mechanism of action of IFIT1, demonstrating that the mRNAs of rubulaviruses can be directly inhibited by IFIT1 and that this is at least partially because their mRNAs are not correctly methylated.
[Mh] Termos MeSH primário: Proteínas de Transporte/farmacologia
Paramyxoviridae/genética
Biossíntese de Proteínas/genética
RNA Mensageiro/genética
Rubulavirus/genética
[Mh] Termos MeSH secundário: Células A549
Linhagem Celular Tumoral
Seres Humanos
Interferon-alfa/metabolismo
Metilação
Vírus da Caxumba/genética
Vírus da Parainfluenza 5/genética
Capuzes de RNA/genética
RNA Viral/genética
Vírus Sendai/genética
Replicação Viral/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (IFIT1 protein, human); 0 (Interferon-alpha); 0 (RNA Caps); 0 (RNA, Messenger); 0 (RNA, Viral)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160812
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.01056-16


  4 / 119 MEDLINE  
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[PMID]:27496728
[Au] Autor:Cuevas-Romero JS; Rivera-Benítez JF; Hernández-Baumgarten E; Hernández-Jaúregui P; Vega M; Blomström AL; Berg M; Baule C
[Ad] Endereço:Centro Nacional de Investigación Disciplinaria en Microbiología Animal, INIFAP, CP. 05110, Mexico City, Mexico; Division of Virology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, S-751 89, Uppsala, Sweden. Electronic address: cuevas.jul
[Ti] Título:Cloning, expression and characterization of potential immunogenic recombinant hemagglutinin-neuraminidase protein of Porcine rubulavirus.
[So] Source:Protein Expr Purif;128:1-7, 2016 Dec.
[Is] ISSN:1096-0279
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Blue eye disease caused by Porcine rubulavirus (PorPV) is an endemic viral infection of swine causing neurological and respiratory disease in piglets, and reproductive failure in sows and boars. The hemagglutinin-neuraminidase (HN) glycoprotein of PorPV is the most abundant component in the viral envelope and the main target of the immune response in infected animals. In this study, we expressed the HN-PorPV-recombinant (rHN-PorPV) protein in an Escherichia coli system and analyzed the immune responses in mice. The HN gene was cloned from the reference strain PorPV-La Piedad Michoacan Virus (GenBank accession number BK005918), into the pDual expression vector. The expressed protein was identified at a molecular weight of 61.7 kDa. Three-dimensional modeling showed that the main conformational and functional domains of the rHN-PorPV protein were preserved. The antigenicity of the expressed protein was confirmed by Western blot with a monoclonal antibody recognizing the HN, and by testing against serum samples from pigs experimentally infected with PorPV. The immunogenicity of the rHN-PorPV protein was tested by inoculation of BALB/c mice with AbISCO-100(®) as adjuvant. Analysis of the humoral immune responses in mice showed an increased level of specific antibodies 14 days after the first immunization, compared to the control group (P < 0.0005). The results show the ability of the rHN-PorPV protein to induce an antibody response in mice. Due to its immunogenic potential, the rHN-PorPV protein will be further evaluated in pig trials for its suitability for prevention and control of blue eye disease.
[Mh] Termos MeSH primário: Clonagem Molecular
Expressão Gênica
Proteína HN
Imunogenicidade da Vacina
Rubulavirus
Vacinas Virais
[Mh] Termos MeSH secundário: Animais
Escherichia coli
Feminino
Proteína HN/biossíntese
Proteína HN/imunologia
Proteína HN/isolamento & purificação
Proteína HN/farmacologia
Camundongos
Camundongos Endogâmicos BALB C
Rubulavirus/enzimologia
Rubulavirus/imunologia
Suínos
Vacinas Virais/biossíntese
Vacinas Virais/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (HN Protein); 0 (Viral Vaccines)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170630
[Lr] Data última revisão:
170630
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160807
[St] Status:MEDLINE


  5 / 119 MEDLINE  
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[PMID]:27479120
[Au] Autor:Peel AJ; Baker KS; Hayman DT; Suu-Ire R; Breed AC; Gembu GC; Lembo T; Fernández-Loras A; Sargan DR; Fooks AR; Cunningham AA; Wood JL
[Ad] Endereço:Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, UK.
[Ti] Título:Bat trait, genetic and pathogen data from large-scale investigations of African fruit bats, Eidolon helvum.
[So] Source:Sci Data;3:160049, 2016 08 01.
[Is] ISSN:2052-4463
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bats, including African straw-coloured fruit bats (Eidolon helvum), have been highlighted as reservoirs of many recently emerged zoonotic viruses. This common, widespread and ecologically important species was the focus of longitudinal and continent-wide studies of the epidemiological and ecology of Lagos bat virus, henipaviruses and Achimota viruses. Here we present a spatial, morphological, demographic, genetic and serological dataset encompassing 2827 bats from nine countries over an 8-year period. Genetic data comprises cytochrome b mitochondrial sequences (n=608) and microsatellite genotypes from 18 loci (n=544). Tooth-cementum analyses (n=316) allowed derivation of rare age-specific serologic data for a lyssavirus, a henipavirus and two rubulaviruses. This dataset contributes a substantial volume of data on the ecology of E. helvum and its viruses and will be valuable for a wide range of studies, including viral transmission dynamic modelling in age-structured populations, investigation of seasonal reproductive asynchrony in wide-ranging species, ecological niche modelling, inference of island colonisation history, exploration of relationships between island and body size, and various spatial analyses of demographic, morphometric or serological data.
[Mh] Termos MeSH primário: Quirópteros/imunologia
Lyssavirus
[Mh] Termos MeSH secundário: Animais
Henipavirus
Nigéria
Rubulavirus
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; COMMENT
[Em] Mês de entrada:1609
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160802
[St] Status:MEDLINE
[do] DOI:10.1038/sdata.2016.49


  6 / 119 MEDLINE  
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[PMID]:26940828
[Au] Autor:Cerriteño-Sánchez JL; Santos-López G; Rosas-Murrieta NH; Reyes-Leyva J; Cuevas-Romero S; Herrera-Camacho I
[Ad] Endereço:Laboratorio de Bioquímica y Biología Molecular, Centro de Química del Instituto de Ciencias (ICUAP), Edificio 103F, Ciudad Universitaria, Benemérita Universidad Autónoma de Puebla, Puebla, Puebla, Mexico; Posgrado en Ciencias Químicas, Edificio FCQ10, Ciudad Universitaria, Benemérita Universidad Aut
[Ti] Título:Production of an enzymatically active and immunogenic form of ectodomain of Porcine rubulavirus hemagglutinin-neuraminidase in the yeast Pichia pastoris.
[So] Source:J Biotechnol;223:52-61, 2016 Apr 10.
[Is] ISSN:1873-4863
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Blue-eye disease (BED) of swine is a viral disease endemic in Mexico. The etiological agent is a paramyxovirus classified as Porcine rubulavirus (PoRV-LPMV), which exhibits in its envelope the hemagglutinin-neuraminidase (HN) glycoprotein, the most immunogenic and a major target for vaccine development. We report in this study the obtaining of ectodomain of PoRV HN (eHN) through the Pichia pastoris expression system. The expression vector (pPICZαB-HN) was integrated by displacement into the yeast chromosome and resulted in a Mut(+) phenotype. Expressed eHN in the P. pastoris X33 strain was recovered from cell-free medium, featuring up to 67 nmol/min/mg after 6 days of expression. eHN was recognized by the serum of infected pigs with strains currently circulating in the Mexican Bajio region. eHN induces antibodies in mice after 28 days of immunization with specific recognition in ELISA test. These antibodies were able to inhibit >80% replication by viral neutralization assays in cell culture. These studies show the obtaining of a protein with similar characteristics to the native HN and which may be a candidate to propose a vaccine or to use the antigen in a serologic diagnostic test.
[Mh] Termos MeSH primário: Proteína HN/química
Pichia/crescimento & desenvolvimento
Proteínas Recombinantes/metabolismo
Rubulavirus/metabolismo
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/metabolismo
Sistema Livre de Células
Proteína HN/genética
Proteína HN/imunologia
Proteína HN/metabolismo
Imunização
Camundongos
Pichia/genética
Domínios Proteicos
Proteínas Recombinantes/genética
Proteínas Recombinantes/imunologia
Rubulavirus/genética
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (HN Protein); 0 (Recombinant Proteins)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160305
[St] Status:MEDLINE


  7 / 119 MEDLINE  
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[PMID]:26854342
[Au] Autor:Rivera-Benitez JF; De la Luz-Armendáriz J; Saavedra-Montañez M; Jasso-Escutia MÁ; Sánchez-Betancourt I; Pérez-Torres A; Reyes-Leyva J; Hernández J; Martínez-Lara A; Ramírez-Mendoza H
[Ad] Endereço:Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Distrito Federal, Mexico; Centro Nacional de Investigación Disciplinaria en Microbiología Animal, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuaria
[Ti] Título:Co-infection of classic swine H1N1 influenza virus in pigs persistently infected with porcine rubulavirus.
[So] Source:Vet Microbiol;184:31-9, 2016 Feb 29.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Porcine rubulavirus (PorPV) and swine influenza virus infection causes respiratory disease in pigs. PorPV persistent infection could facilitate the establishment of secondary infections. The aim of this study was to analyse the pathogenicity of classic swine H1N1 influenza virus (swH1N1) in growing pigs persistently infected with porcine rubulavirus. Conventional six-week-old pigs were intranasally inoculated with PorPV, swH1N1, or PorPV/swH1N1. A mock-infected group was included. The co-infection with swH1N1 was at 44 days post-infection (DPI), right after clinical signs of PorPV infection had stopped. The pigs of the co-infection group presented an increase of clinical signs compared to the simple infection groups. In all infected groups, the most recurrent lung lesion was hyperplasia of the bronchiolar-associated lymphoid tissue and interstitial pneumonia. By means of immunohistochemical evaluation it was possible to demonstrate the presence of the two viral agents infecting simultaneously the bronchiolar epithelium. Viral excretion of PorPV in nasal and oral fluid was recorded at 28 and 52 DPI, respectively. PorPV persisted in several samples from respiratory tissues (RT), secondary lymphoid organs (SLO), and bronchoalveolar lavage fluid (BALF). For swH1N1, the viral excretion in nasal fluids was significantly higher in single-infected swH1N1 pigs than in the co-infected group. However, the co-infection group exhibited an increase in the presence of swH1N1 in RT, SLO, and BALF at two days after co-infection. In conclusion, the results obtained confirm an increase in the clinical signs of infection, and PorPV was observed to impact the spread of swH1N1 in analysed tissues in the early stage of co-infection, although viral shedding was not enhanced. In the present study, the interaction of swH1N1 infection is demonstrated in pigs persistently infected with PorPV.
[Mh] Termos MeSH primário: Coinfecção/patologia
Vírus da Influenza A Subtipo H1N1/patogenicidade
Infecções por Orthomyxoviridae/veterinária
Infecções por Rubulavirus/veterinária
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/sangue
Vírus da Influenza A Subtipo H1N1/isolamento & purificação
Infecções por Orthomyxoviridae/complicações
Infecções por Orthomyxoviridae/patologia
Infecções por Orthomyxoviridae/virologia
Rubulavirus/isolamento & purificação
Rubulavirus/fisiologia
Infecções por Rubulavirus/complicações
Infecções por Rubulavirus/patologia
Infecções por Rubulavirus/virologia
Suínos
Doenças dos Suínos/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160209
[St] Status:MEDLINE


  8 / 119 MEDLINE  
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[PMID]:26728078
[Au] Autor:Cuevas-Romero S; Rivera-Benítez JF; Blomström AL; Ramliden M; Hernández-Baumgarten E; Hernández-Jáuregui P; Ramírez-Mendoza H; Berg M
[Ad] Endereço:Centro Nacional de Investigación Disciplinaria en Microbiología Animal, INIFAP, Mexico City, Mexico. cuevas.julieta@inifap.gob.mx.
[Ti] Título:Molecular characterisation of Porcine rubulavirus (PorPV) isolates from different outbreaks in Mexico.
[So] Source:Virus Genes;52(1):81-90, 2016 Feb.
[Is] ISSN:1572-994X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Since the report of the initial outbreak of Porcine rubulavirus (PorPV) infection in pigs, only one full-length genome from 1984 (PorPV-LPMV/1984) has been characterised. To investigate the overall genetic variation, full-length gene nucleotide sequences of current PorPV isolates were obtained from different clinical cases of infected swine. Genome organisation and sequence analysis of the encoded proteins (NP, P, F, M, HN and L) revealed high sequence conservation of the NP protein and the expression of the P and V proteins in all PorPV isolates. The V protein of one isolate displayed a mutation that has been implicated to antagonise the antiviral immune responses of the host. The M protein indicated a variation in a short region that could affect the electrostatic charge and the interaction with the membrane. One PorPV isolate recovered from the lungs showed a mutation at the cleavage site (HRKKR) of the F protein that could represent an important factor to determine the tissue tropism and pathogenicity of this virus. The HN protein showed high sequence identity through the years (up to 2013). Additionally, a number of sequence motifs of very high amino acid conservation among the PorPV isolates important for polymerase activity of the L protein have been identified. In summary, genetic comparisons and phylogenetic analyses indicated that three different genetic variants of PorPV are currently spreading within the swine population, and a new generation of circulating virus with different characteristics has begun to emerge.
[Mh] Termos MeSH primário: Infecções por Rubulavirus/veterinária
Rubulavirus/genética
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Animais
DNA Complementar
Surtos de Doenças/veterinária
Genes Virais
Variação Genética
México/epidemiologia
Filogenia
RNA Viral
Rubulavirus/classificação
Infecções por Rubulavirus/epidemiologia
Infecções por Rubulavirus/virologia
Análise de Sequência de RNA
Suínos
Doenças dos Suínos/epidemiologia
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (RNA, Viral); 0 (Viral Proteins)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160106
[St] Status:MEDLINE
[do] DOI:10.1007/s11262-015-1281-y


  9 / 119 MEDLINE  
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[PMID]:26546155
[Au] Autor:Pisanelli G; Laurent-Rolle M; Manicassamy B; Belicha-Villanueva A; Morrison J; Lozano-Dubernard B; Castro-Peralta F; Iovane G; García-Sastre A
[Ad] Endereço:Department of Microbiology, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, NY 10029, United States; Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, NY 10029, United States; Department of Vete
[Ti] Título:La Piedad Michoacán Mexico Virus V protein antagonizes type I interferon response by binding STAT2 protein and preventing STATs nuclear translocation.
[So] Source:Virus Res;213:11-22, 2016 Feb 02.
[Is] ISSN:1872-7492
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:La Piedad Michoacán Mexico Virus (LPMV) is a member of the Rubulavirus genus within the Paramyxoviridae family. LPMV is the etiologic agent of "blue eye disease", causing a significant disease burden in swine in Mexico with long-term implications for the agricultural industry. This virus mainly affects piglets and is characterized by meningoencephalitis and respiratory distress. It also affects adult pigs, causing reduced fertility and abortions in females, and orchitis and epididymitis in males. Viruses of the Paramyxoviridae family evade the innate immune response by targeting components of the interferon (IFN) signaling pathway. The V protein, expressed by most paramyxoviruses, is a well-characterized IFN signaling antagonist. Until now, there were no reports on the role of the LPMV-V protein in inhibiting the IFN response. In this study we demonstrate that LPMV-V protein antagonizes type I but not type II IFN signaling by binding STAT2, a component of the type I IFN cascade. Our results indicate that the last 18 amino acids of LPMV-V protein are required for binding to STAT2 in human and swine cells. While LPMV-V protein does not affect the protein levels of STAT1 or STAT2, it does prevent the IFN-induced phosphorylation and nuclear translocation of STAT1 and STAT2 thereby inhibiting cellular responses to IFN α/ß.
[Mh] Termos MeSH primário: Interferon Tipo I/antagonistas & inibidores
Rubulavirus/imunologia
Fator de Transcrição STAT2/metabolismo
Proteínas Virais/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Seres Humanos
Ligação Proteica
Transporte Proteico
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Interferon Type I); 0 (STAT2 Transcription Factor); 0 (Viral Proteins); 147652-41-3 (V protein, Porcine rubulavirus)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:170812
[Lr] Data última revisão:
170812
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151108
[St] Status:MEDLINE


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[PMID]:26334289
[Au] Autor:Arvia R; Corcioli F; Ciccone N; Della Malva N; Azzi A
[Ad] Endereço:Department of Experimental and Clinical Medicine, University of Florence, Viale Morgagni 48, 50134 Florence, Italy. Electronic address: rosaria.arvia@libero.it.
[Ti] Título:Detection of 12 respiratory viruses by duplex real time PCR assays in respiratory samples.
[So] Source:Mol Cell Probes;29(6):408-413, 2015 Dec.
[Is] ISSN:1096-1194
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Different viruses can be responsible for similar clinical manifestations of respiratory infections. Thus, the etiological diagnosis of respiratory viral diseases requires the detection of a large number of viruses. In this study, 6 duplex real-time PCR assays, using EvaGreen intercalating dye, were developed to detect 12 major viruses responsible for respiratory diseases: influenza A and B viruses, enteroviruses (including enterovirus spp, and rhinovirus spp), respiratory syncytial virus, human metapneumovirus, coronaviruses group I (of which CoV 229E and CoV NL63 are part) and II (including CoV OC43 and CoV HKU1), parainfluenza viruses type 1, 2, 3 and 4, human adenoviruses and human bocaviruses. The 2 target viruses of each duplex reaction were distinguishable by the melting temperatures of their amplicons. The 6 duplex real time PCR assays were applied for diagnostic purpose on 202 respiratory samples from 157 patients. One hundred fifty-seven samples were throat swabs and 45 were bronchoalveolar lavages. The results of the duplex PCR assays were confirmed by comparison with a commercial, validated, assay; in addition, the positive results were confirmed by sequencing. The analytical sensitivity of the duplex PCR assays varied from 10(3) copies/ml to 10(4) copies/ml. For parainfluenza virus 2 only it was 10(5) copies/ml. Seventy clinical samples (35%) from 55 patients (30 children and 25 adults) were positive for 1 or more viruses. In adult patients, influenza A virus was the most frequently detected respiratory virus followed by rhinoviruses. In contrast, respiratory syncytial virus was the most common virus in children, followed by enteroviruses, influenza A virus and coronavirus NL63. The small number of samples/patients does not allow us to draw any epidemiological conclusion. Altogether, the results of this study indicate that the 6 duplex PCR assays described in this study are sensitive, specific and cost-effective. Thus, this assay could be particularly useful to identify the main respiratory viruses directly from clinical samples, after nucleic acid extraction, and, also, to screen a large number of patients for epidemiological studies.
[Mh] Termos MeSH primário: Adenoviridae/isolamento & purificação
Bocavirus Humano/isolamento & purificação
Reação em Cadeia da Polimerase Multiplex/métodos
Vírus de RNA/isolamento & purificação
Infecções Respiratórias/virologia
[Mh] Termos MeSH secundário: Adenoviridae/classificação
Coronavirus/classificação
Coronavirus/isolamento & purificação
Enterovirus/classificação
Enterovirus/isolamento & purificação
Bocavirus Humano/classificação
Seres Humanos
Vírus da Influenza A/classificação
Vírus da Influenza A/isolamento & purificação
Vírus da Influenza B/classificação
Vírus da Influenza B/isolamento & purificação
Metapneumovirus/classificação
Metapneumovirus/isolamento & purificação
Vírus de RNA/classificação
Vírus Sinciciais Respiratórios/classificação
Vírus Sinciciais Respiratórios/isolamento & purificação
Respirovirus/classificação
Respirovirus/isolamento & purificação
Rubulavirus/classificação
Rubulavirus/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1610
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150904
[St] Status:MEDLINE



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