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[PMID]:29036182
[Au] Autor:Hägglund S; Blodörn K; Näslund K; Vargmar K; Lind SB; Mi J; Araínga M; Riffault S; Taylor G; Pringle J; Valarcher JF
[Ad] Endereço:Swedish University of Agricultural Sciences, Host Pathogen Interaction Group, Dept. of Clinical Sciences, Uppsala, Sweden.
[Ti] Título:Proteome analysis of bronchoalveolar lavage from calves infected with bovine respiratory syncytial virus-Insights in pathogenesis and perspectives for new treatments.
[So] Source:PLoS One;12(10):e0186594, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Human and bovine respiratory syncytial viruses (HRSV/BRSV) are major causes of severe lower respiratory tract infections in children and calves, respectively. Shared epidemiological, clinical, pathological and genetic characteristics of these viruses make comparative research highly relevant. To characterise the host response against BRSV infection, bronchoalveolar lavage supernatant (BAL) from i) non-vaccinated, BRSV-infected ii) vaccinated, BRSV-infected and iii) non-infected calves was analysed by tandem mass spectrometry. Proteins were semi-quantified and protein expression was validated by immunoblotting. Correlations between selected proteins and pathology, clinical signs and virus shedding were investigated. Calves with BRSV-induced disease had increased total protein concentrations and a decreased number of proteins identified in BAL. The protein profile was characterised by neutrophil activation and a reduction in identified antioxidant enzymes. The presence of neutrophils in alveolar septa, the expression level of neutrophil-related or antioxidant proteins and LZTFL1 correlated significantly with disease. Citrullinated histone 3, an indicator of extracellular traps (ETs), was only detected in non-vaccinated, BRSV-infected animals. By bringing disequilibrium in the release and detoxification of reactive oxygen species, generating ETs and causing elastine degradation, exaggerated neutrophil responses might exacerbate RSV-induced disease. Neutrophil-mitigating or antioxidant treatments should be further explored.
[Mh] Termos MeSH primário: Lavagem Broncoalveolar
Proteômica
Infecções por Vírus Respiratório Sincicial/metabolismo
Infecções por Vírus Respiratório Sincicial/terapia
Vírus Sincicial Respiratório Bovino/fisiologia
[Mh] Termos MeSH secundário: Animais
Bovinos
Ativação de Neutrófilo
Neutrófilos/imunologia
Espécies Reativas de Oxigênio/metabolismo
Infecções por Vírus Respiratório Sincicial/etiologia
Infecções por Vírus Respiratório Sincicial/imunologia
Sistema Respiratório/virologia
Transcriptoma
Eliminação de Partículas Virais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Reactive Oxygen Species)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171017
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186594


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[PMID]:28714847
[Au] Autor:Pollock N; Taylor G; Jobe F; Guzman E
[Ad] Endereço:1​School of Biosciences, Cardiff University, Cardiff, CF10 3AX, UK 2​The Pirbright Institute, Ash Road, Pirbright, Woking, RG8 0JU, UK.
[Ti] Título:Modulation of the transcription factor NF-κB in antigen-presenting cells by bovine respiratory syncytial virus small hydrophobic protein.
[So] Source:J Gen Virol;98(7):1587-1599, 2017 Jul.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bovine respiratory syncytial virus (BRSV) is an important cause of respiratory disease in young cattle and is closely related to human RSV (HRSV), which causes severe respiratory disease in infants and the elderly. The RSV genome encodes a small hydrophobic (SH) protein with viroporin activity. Previous studies have shown that recombinant BRSV lacking the SH gene (rBRSVΔSH) is attenuated in the lungs, but not in the upper respiratory tract, of calves and mucosal vaccination with rBRSVΔSH induced long-lasting protective immunity. Attenuation of rBRSVΔSH may be due to the ability of this virus to induce an early innate response as rBRSVΔSH induces higher levels of pro-inflammatory cytokines than wild-type (wt) rBRSV. In this study, we investigated the effects of the BRSV SH protein on NF-κB p65 phosphorylation, a master step in the regulation of pro-inflammatory cytokines. Expression of SH resulted in the inhibition of NF-κB p65 phosphorylation in response to BRSV infection and extracellular lipopolysaccharide, and a reduction in the production of pro-inflammatory cytokines. In contrast, rBRSVΔSH does not inhibit NF-κB p65 phosphorylation in bovine antigen-presenting cells, including monocytes, macrophages and dendritic cells, resulting in increased expression of pro-inflammatory cytokines and increased activation of T cells compared to cells infected with wt BRSV. These findings highlight an important role for the BRSV SH protein in immune modulation.
[Mh] Termos MeSH primário: Citocinas/metabolismo
Células Dendríticas/imunologia
Macrófagos/imunologia
Monócitos/imunologia
Vírus Sincicial Respiratório Bovino/metabolismo
Proteínas Oncogênicas de Retroviridae/imunologia
Fator de Transcrição RelA/metabolismo
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos/imunologia
Doenças dos Bovinos/virologia
Linhagem Celular
Células Dendríticas/metabolismo
Células Dendríticas/virologia
Seres Humanos
Lipopolissacarídeos/metabolismo
Ativação Linfocitária/imunologia
Macrófagos/metabolismo
Macrófagos/virologia
Camundongos
Monócitos/metabolismo
Monócitos/virologia
Inibidor de NF-kappaB alfa/metabolismo
Fosforilação
Células RAW 264.7
Vírus Sincicial Respiratório Bovino/genética
Vírus Sincicial Respiratório Bovino/imunologia
Proteínas Oncogênicas de Retroviridae/genética
Proteínas Oncogênicas de Retroviridae/metabolismo
Linfócitos T/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Lipopolysaccharides); 0 (Retroviridae Proteins, Oncogenic); 0 (Transcription Factor RelA); 0 (small hydrophobic protein, virus); 139874-52-5 (NF-KappaB Inhibitor alpha)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171115
[Lr] Data última revisão:
171115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000855


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[PMID]:28432927
[Au] Autor:Alkheraif AA; Topliff CL; Reddy J; Massilamany C; Donis RO; Meyers G; Eskridge KM; Kelling CL
[Ad] Endereço:University of Nebraska, School of Veterinary Medicine and Biomedical Sciences, 1880 North 42nd Street, Lincoln, NE, 68583, United States.
[Ti] Título:Type 2 BVDV N suppresses IFN-1 pathway signaling in bovine cells and augments BRSV replication.
[So] Source:Virology;507:123-134, 2017 Jul.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bovine viral diarrhea virus (BVDV) infection induces immunosuppression and in conjunction with bovine respiratory syncytial virus (BRSV) contributes to the bovine respiratory disease complex. Bovine turbinate cells were single or co-infected with type 2 BVDV wild-type (BVDV2-wt), its dysfunctional N mutant (BVDV2-E), and/or BRSV. BVDV2-E significantly up-regulated PKR, IRF-7, TBK-1, IRF-3, and IFN-ß mRNAs based on real-time Q-RT-PCR. BRSV-infected cells expressed significantly up-regulated PKR, IRF-3, IRF-7, and IFN-ß mRNAs, whereas BVDV2-wt, but not BVDV2-E, abolished this up-regulation in co-infection. No significant differences were observed in MAVS, NF-κB, and PIN-1 mRNAs. A dual-luciferase reporter assay showed that BVDV2-wt significantly increased NF-κB activity compared to BVDV2-E, while BVDV2-E significantly increased IFN-ß activity compared to BVDV2-wt. The BRSV titer and RNA levels significantly increased in cells co-infected with BRSV/BVDV2-wt compared to cells co-infected with BRSV/BVDV2-E or infected with BRSV alone. This data supports the synergistic action of BVDV2-wt and BRSV inhibition of IFN-1.
[Mh] Termos MeSH primário: Doença das Mucosas por Vírus da Diarreia Viral Bovina/metabolismo
Vírus da Diarreia Viral Bovina Tipo 2/fisiologia
Interferon beta/metabolismo
Infecções por Vírus Respiratório Sincicial/veterinária
Transdução de Sinais
[Mh] Termos MeSH secundário: Animais
Doença das Mucosas por Vírus da Diarreia Viral Bovina/genética
Doença das Mucosas por Vírus da Diarreia Viral Bovina/virologia
Bovinos
Coinfecção/genética
Coinfecção/imunologia
Coinfecção/virologia
Vírus da Diarreia Viral Bovina Tipo 2/genética
Interferon beta/genética
NF-kappa B/genética
NF-kappa B/metabolismo
Infecções por Vírus Respiratório Sincicial/genética
Infecções por Vírus Respiratório Sincicial/imunologia
Infecções por Vírus Respiratório Sincicial/virologia
Vírus Sincicial Respiratório Bovino/genética
Vírus Sincicial Respiratório Bovino/fisiologia
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (NF-kappa B); 77238-31-4 (Interferon-beta)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170717
[Lr] Data última revisão:
170717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170423
[St] Status:MEDLINE


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[PMID]:28295570
[Au] Autor:Doyle D; Credille B; Lehenbauer TW; Berghaus R; Aly SS; Champagne J; Blanchard P; Crossley B; Berghaus L; Cochran S; Woolums A
[Ad] Endereço:Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA.
[Ti] Título:Agreement Among 4 Sampling Methods to Identify Respiratory Pathogens in Dairy Calves with Acute Bovine Respiratory Disease.
[So] Source:J Vet Intern Med;31(3):954-959, 2017 May.
[Is] ISSN:1939-1676
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Four sampling techniques commonly are used for antemortem identification of pathogens from cattle with bovine respiratory disease (BRD): the nasal swab (NS), guarded nasopharyngeal swab (NPS), bronchoalveolar lavage (BAL), and transtracheal wash (TTW). Agreement among these methods has not been well characterized. OBJECTIVE: To evaluate agreement among TTW and NS, NPS, or BAL for identification of viral and bacterial pathogens in dairy calves with BRD. ANIMALS: One hundred dairy calves with naturally acquired BRD. METHODS: Calves were sampled by all 4 methods. Viral agents were identified by real-time RT-PCR, bacteria were identified by aerobic culture, and Mycoplasma bovis (M. bovis) isolates were speciated by PCR. Agreement among TTW and NS, NPS, or BAL was evaluated by calculating the kappa statistic and percent positive agreement. McNemar's exact test was used to compare the proportions of positive results. RESULTS: Agreement among TTW and NS, TTW and NPS, and TTW and BAL, was very good for identification of P. multocida, M. haemolytica, and M. bovis. For bovine respiratory syncytial virus (BRSV), agreement with TTW was moderate for NS, good for NPS, and very good for BAL. For bovine coronavirus (BCV), agreement with TTW was moderate for NS and NPS, and good for BAL. McNemar's test was significant only for BCV, indicating that for this pathogen the proportion of positive results from NS and NPS could not be considered comparable to TTW. CONCLUSIONS AND CLINICAL IMPORTANCE: This study provides guidance for veterinarians selecting diagnostic tests for antemortem identification of pathogens associated with BRD.
[Mh] Termos MeSH primário: Doenças dos Bovinos/diagnóstico
Infecções Respiratórias/veterinária
[Mh] Termos MeSH secundário: Doença Aguda
Animais
Lavagem Broncoalveolar/veterinária
Bovinos
Doenças dos Bovinos/microbiologia
Doenças dos Bovinos/virologia
Coronavirus
Feminino
Mycoplasma bovis
Mucosa Nasal/microbiologia
Nasofaringe/microbiologia
Vírus Sincicial Respiratório Bovino
Infecções Respiratórias/diagnóstico
Infecções Respiratórias/microbiologia
Infecções Respiratórias/virologia
Traqueia/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170316
[St] Status:MEDLINE
[do] DOI:10.1111/jvim.14683


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[PMID]:28282443
[Au] Autor:Murray GM; O'Neill RG; Lee AM; McElroy MC; More SJ; Monagle A; Earley B; Cassidy JP
[Ad] Endereço:Sligo Regional Veterinary Laboratory, Department of Agriculture, Food and Marine, Doonally, Sligo, Ireland.
[Ti] Título:The bovine paranasal sinuses: Bacterial flora, epithelial expression of nitric oxide and potential role in the in-herd persistence of respiratory disease pathogens.
[So] Source:PLoS One;12(3):e0173845, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The bovine paranasal sinuses are a group of complex cavernous air-filled spaces, lined by respiratory epithelium, the exact function of which is unclear. While lesions affecting these sinuses are occasionally reported in cattle, their microbial flora has not been defined. Furthermore, given that the various bacterial and viral pathogens causing bovine respiratory disease (BRD) persist within herds, we speculated that the paranasal sinuses may serve as a refuge for such infectious agents. The paranasal sinuses of clinically normal cattle (n = 99) and of cattle submitted for post-mortem examination (PME: n = 34) were examined by microbial culture, PCR and serology to include bacterial and viral pathogens typically associated with BRD: Mycoplasma bovis, Histophilus somni, Mannheimia haemolytica and Pasteurella multocida, bovine respiratory syncytial virus (BRSV) and bovine parainfluenza-3 virus (BPIV-3). Overall, the paranasal sinuses were either predominantly sterile or did not contain detectable microbes (83.5%: 94.9% of clinically normal and 50.0% of cattle submitted for PME). Bacteria, including BRD causing pathogens, were identified in relatively small numbers of cattle (<10%). While serology indicated widespread exposure of both clinically normal and cattle submitted for PME to BPIV-3 and BRSV (seroprevalences of 91.6% and 84.7%, respectively), PCR identified BPIV-3 in only one animal. To further explore these findings we investigated the potential role of the antimicrobial molecule nitric oxide (NO) within paranasal sinus epithelium using immunohistochemistry. Expression of the enzyme responsible for NO synthesis, inducible nitric oxide synthase (iNOS), was detected to varying degrees in 76.5% of a sub-sample of animals suggesting production of this compound plays a similar protective role in the bovine sinus as it does in humans.
[Mh] Termos MeSH primário: Complexo Respiratório Bovino/virologia
Microbiota
Óxido Nítrico/metabolismo
Seios Paranasais/microbiologia
[Mh] Termos MeSH secundário: Animais
Bactérias/genética
Bactérias/patogenicidade
Complexo Respiratório Bovino/microbiologia
Bovinos
Estudos Transversais
Células Epiteliais/metabolismo
Feminino
Masculino
Microbiota/genética
Óxido Nítrico Sintase Tipo II/metabolismo
Vírus da Parainfluenza 3 Bovina/genética
Vírus da Parainfluenza 3 Bovina/isolamento & purificação
Vírus da Parainfluenza 3 Bovina/patogenicidade
Seios Paranasais/metabolismo
Vírus Sincicial Respiratório Bovino/genética
Vírus Sincicial Respiratório Bovino/isolamento & purificação
Vírus Sincicial Respiratório Bovino/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
31C4KY9ESH (Nitric Oxide); EC 1.14.13.39 (Nitric Oxide Synthase Type II)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173845


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[PMID]:28074713
[Au] Autor:Murray GM; More SJ; Sammin D; Casey MJ; McElroy MC; O'Neill RG; Byrne WJ; Earley B; Clegg TA; Ball H; Bell CJ; Cassidy JP
[Ad] Endereço:Sligo Regional Veterinary Laboratory, Department of Agriculture, Food and Marine, Doonally, Sligo, Ireland (Murray).
[Ti] Título:Pathogens, patterns of pneumonia, and epidemiologic risk factors associated with respiratory disease in recently weaned cattle in Ireland.
[So] Source:J Vet Diagn Invest;29(1):20-34, 2017 Jan.
[Is] ISSN:1943-4936
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We examined the pathogens, morphologic patterns, and risk factors associated with bovine respiratory disease (BRD) in 136 recently weaned cattle ("weanlings"), 6-12 mo of age, that were submitted for postmortem examination to regional veterinary laboratories in Ireland. A standardized sampling protocol included routine microbiologic investigations as well as polymerase chain reaction and immunohistochemistry. Lungs with histologic lesions were categorized into 1 of 5 morphologic patterns of pneumonia. Fibrinosuppurative bronchopneumonia (49%) and interstitial pneumonia (48%) were the morphologic patterns recorded most frequently. The various morphologic patterns of pulmonary lesions suggest the involvement of variable combinations of initiating and compounding infectious agents that hindered any simple classification of the etiopathogenesis of the pneumonias. Dual infections were detected in 58% of lungs, with Mannheimia haemolytica and Histophilus somni most frequently recorded in concert. M. haemolytica (43%) was the most frequently detected respiratory pathogen; H. somni was also shown to be frequently implicated in pneumonia in this age group of cattle. Bovine parainfluenza virus 3 (BPIV-3) and Bovine respiratory syncytial virus (16% each) were the viral agents detected most frequently. Potential respiratory pathogens (particularly Pasteurella multocida, BPIV-3, and H. somni) were frequently detected (64%) in lungs that had neither gross nor histologic pulmonary lesions, raising questions regarding their role in the pathogenesis of BRD. The breadth of respiratory pathogens detected in bovine lungs by various detection methods highlights the diagnostic value of parallel analyses in respiratory disease postmortem investigation.
[Mh] Termos MeSH primário: Broncopneumonia/veterinária
Doenças dos Bovinos/epidemiologia
[Mh] Termos MeSH secundário: Animais
Animais Lactentes
Autopsia/veterinária
Broncopneumonia/epidemiologia
Bovinos
Doenças dos Bovinos/diagnóstico
Doenças dos Bovinos/microbiologia
Imuno-Histoquímica/veterinária
Irlanda/epidemiologia
Mannheimia haemolytica/isolamento & purificação
Vírus da Parainfluenza 3 Bovina/isolamento & purificação
Pasteurella multocida/isolamento & purificação
Reação em Cadeia da Polimerase/veterinária
Vírus Sincicial Respiratório Bovino/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170112
[St] Status:MEDLINE
[do] DOI:10.1177/1040638716674757


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[PMID]:27720029
[Au] Autor:Toftaker I; Sanchez J; Stokstad M; Nødtvedt A
[Ad] Endereço:Department of Production Animal Clinical Sciences, Faculty of Veterinary Medicine and Biosciences, Norwegian University of Life Sciences, P.O. Box 8146 Dep, Oslo, Norway. Electronic address: ingrid.toftaker@nmbu.no.
[Ti] Título:Bovine respiratory syncytial virus and bovine coronavirus antibodies in bulk tank milk - risk factors and spatial analysis.
[So] Source:Prev Vet Med;133:73-83, 2016 Oct 01.
[Is] ISSN:1873-1716
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Bovine respiratory syncytial virus (BRSV) and bovine coronavirus (BCoV) are considered widespread among cattle in Norway and worldwide. This cross-sectional study was conducted based on antibody-ELISA of bulk tank milk (BTM) from 1347 herds in two neighboring counties in western Norway. The study aims were to determine the seroprevalence at herd level, to evaluate risk factors for BRSV and BCoV seropositivity, and to assess how these factors were associated with the spatial distribution of positive herds. The overall prevalence of BRSV and BCoV positive herds in the region was 46.2% and 72.2%, respectively. Isopleth maps of the prevalence risk distribution showed large differences in prevalence risk across the study area, with the highest prevalence in the northern region. Common risk factors of importance for both viruses were herd size, geographic location, and proximity to neighbors. Seropositivity for one virus was associated with increased odds of seropositivity for the other virus. Purchase of livestock was an additional risk factor for BCoV seropositivity, included in the model as in-degree, which was defined as the number of incoming movements from individual herds, through animal purchase, over a period of five years. Local dependence and the contribution of risk factors to this effect were assessed using the residuals from two logistic regression models for each virus. One model contained only the x- and y- coordinates as predictors, the other had all significant predictors included. Spatial clusters of high values of residuals were detected using the normal model of the spatial scan statistic and visualized on maps. Adjusting for the risk factors in the final models had different impact on the spatial clusters for the two viruses: For BRSV the number of clusters was reduced from six to four, for BCoV the number of clusters remained the same, however the log-likelihood ratios changed notably. This indicates that geographical differences in proximity to neighbors, herd size and animal movements explain some of the spatial clusters of BRSV- and BCoV seropositivity, but far from all. The remaining local dependence in the residuals show that the antibody status of one herd is influenced by the antibody status of its neighbors, indicating the importance of indirect transmission and that increased biosecurity routines might be an important preventive strategy.
[Mh] Termos MeSH primário: Doenças dos Bovinos/epidemiologia
Infecções por Coronavirus/veterinária
Coronavirus Bovino/isolamento & purificação
Leite/virologia
Infecções por Vírus Respiratório Sincicial/veterinária
Vírus Sincicial Respiratório Bovino/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/análise
Bovinos
Doenças dos Bovinos/virologia
Infecções por Coronavirus/epidemiologia
Infecções por Coronavirus/virologia
Estudos Transversais
Ensaio de Imunoadsorção Enzimática/veterinária
Modelos Logísticos
Noruega/epidemiologia
Prevalência
Infecções por Vírus Respiratório Sincicial/epidemiologia
Infecções por Vírus Respiratório Sincicial/virologia
Fatores de Risco
Estudos Soroepidemiológicos
Análise Espacial
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170412
[Lr] Data última revisão:
170412
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161011
[St] Status:MEDLINE


  8 / 375 MEDLINE  
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[PMID]:27496747
[Au] Autor:Palomares RA; Hurley DJ; Bittar JH; Saliki JT; Woolums AR; Moliere F; Havenga LJ; Norton NA; Clifton SJ; Sigmund AB; Barber CE; Berger ML; Clark MJ; Fratto MA
[Ad] Endereço:Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA 30602 United States; Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens GA 30602 United States. Electronic address: palomnr@uga.edu.
[Ti] Título:Effects of injectable trace minerals on humoral and cell-mediated immune responses to Bovine viral diarrhea virus, Bovine herpes virus 1 and Bovine respiratory syncytial virus following administration of a modified-live virus vaccine in dairy calves.
[So] Source:Vet Immunol Immunopathol;178:88-98, 2016 Oct 01.
[Is] ISSN:1873-2534
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Our objective was to evaluate the effect of an injectable trace mineral (ITM) supplement containing zinc, manganese, selenium, and copper on the humoral and cell mediated immune (CMI) responses to vaccine antigens in dairy calves receiving a modified-live viral (MLV) vaccine containing BVDV, BHV1, PI3V and BRSV. A total of 30 dairy calves (3.5 months of age) were administered a priming dose of the MLV vaccine containing BHV1, BVDV1 & 2, BRSV, PI3V, and an attenuated-live Mannheimia-Pasteurella bacterin subcutaneously (SQ). Calves were randomly assigned to 1 of 2 groups: (1) administration of ITM SQ (ITM, n=15) or (2) injection of sterile saline SQ (Control; n=15). Three weeks later, calves received a booster of the same vaccine combination SQ, and a second administration of ITM, or sterile saline, according to the treatment group. Blood samples were collected on days 0, 7, 14, 21, 28, 42, 56, and 90 post-vaccination for determination of antibody titer, viral recall antigen-induced IFN-γ production, and viral antigen-induced proliferation by peripheral blood mononuclear cells (PBMC). Administration of ITM concurrently with MLV vaccination resulted in higher antibody titers to BVDV1 on day 28 after priming vaccination compared to the control group (P=0.03). Calves treated with ITM showed an earlier enhancement in PBMC proliferation to BVDV1 following vaccination compared to the control group. Proliferation of PBMC after BVDV stimulation tended to be higher on day 14 after priming vaccination in calves treated with ITM than in the control group (P=0.08). Calves that received ITM showed higher PBMC proliferation to BRSV stimulation on day 7 after priming vaccination compared to the control group (P=0.01). Moreover, calves in the ITM group also had an enhanced production IFN-γ by PBMC after stimulation with BRSV on day 21 after priming vaccination compared to day 0 (P<0.01). In conclusion, administration of ITM concurrently with MLV vaccination in dairy calves resulted in increased antibody titer to BVDV1, and greater PBMC proliferation to BVDV1 and BRSV recall stimulation compared to the control group, suggesting that ITM might represent a promising tool to enhance the humoral and CMI responses to MLV vaccines in cattle.
[Mh] Termos MeSH primário: Doenças dos Bovinos/imunologia
Doenças dos Bovinos/prevenção & controle
Vírus da Diarreia Viral Bovina/imunologia
Herpesvirus Bovino 1/imunologia
Vírus Sincicial Respiratório Bovino/imunologia
Oligoelementos/administração & dosagem
Vacinas Virais/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Anticorpos Neutralizantes/sangue
Anticorpos Antivirais/sangue
Doença das Mucosas por Vírus da Diarreia Viral Bovina/imunologia
Doença das Mucosas por Vírus da Diarreia Viral Bovina/prevenção & controle
Bovinos
Infecções por Herpesviridae/imunologia
Infecções por Herpesviridae/prevenção & controle
Infecções por Herpesviridae/veterinária
Imunidade Celular/efeitos dos fármacos
Imunidade Humoral/efeitos dos fármacos
Masculino
Infecções por Vírus Respiratório Sincicial/imunologia
Infecções por Vírus Respiratório Sincicial/prevenção & controle
Infecções por Vírus Respiratório Sincicial/veterinária
Vacinas Atenuadas/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Antibodies, Neutralizing); 0 (Antibodies, Viral); 0 (Trace Elements); 0 (Vaccines, Attenuated); 0 (Viral Vaccines)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160807
[St] Status:MEDLINE


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[PMID]:27108192
[Au] Autor:Fulton RW; d'Offay JM; Landis C; Miles DG; Smith RA; Saliki JT; Ridpath JF; Confer AW; Neill JD; Eberle R; Clement TJ; Chase CC; Burge LJ; Payton ME
[Ad] Endereço:Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK 74078, USA. Electronic address: Robert.fulton@okstate.edu.
[Ti] Título:Detection and characterization of viruses as field and vaccine strains in feedlot cattle with bovine respiratory disease.
[So] Source:Vaccine;34(30):3478-92, 2016 Jun 24.
[Is] ISSN:1873-2518
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This study investigated viruses in bovine respiratory disease (BRD) cases in feedlots, including bovine herpesvirus-1 (BoHV-1), bovine viral diarrhea virus (BVDV), bovine respiratory syncytial virus (BRSV), bovine coronaviruses (BoCV) and parainfluenza-3 virus (PI3V). Nasal swabs were collected from 114 cattle on initial BRD treatment. Processing included modified live virus (MLV) vaccination. Seven BRD necropsy cases were included for 121 total cases. Mean number of days on feed before first sample was 14.9 days. Swabs and tissue homogenates were tested by gel based PCR (G-PCR), quantitative-PCR (qPCR) and quantitative real time reverse transcriptase PCR (qRT-PCR) and viral culture. There were 87/114 (76.3%) swabs positive for at least one virus by at least one test. All necropsy cases were positive for at least one virus. Of 121 cases, positives included 18/121 (14.9%) BoHV-1; 19/121 (15.7%) BVDV; 76/121 (62.8%) BoCV; 11/121 (9.1%) BRSV; and 10/121 (8.3%) PI3V. For nasal swabs, G-PCR (5 viruses) detected 44/114 (38.6%); q-PCR and qRT-PCR (4 viruses) detected 81/114 (71.6%); and virus isolation detected 40/114 (35.1%). Most were positive for only one or two tests, but not all three tests. Necropsy cases had positives: 5/7 G-PCR, 5/7 q-PCR and qRT-PCR, and all were positive by cell culture. In some cases, G-PCR and both real time PCR were negative for BoHV-1, BVDV, and PI3V in samples positive by culture. PCR did not differentiate field from vaccines strains of BoHV-1, BVDV, and PI3V. However based on sequencing and analysis, field and vaccine strains of culture positive BoHV-1, BoCV, BVDV, and PI3V, 11/18 (61.1%) of BoHV-1 isolates, 6/17 (35.3%) BVDV isolates, and 1/10 (10.0%) PI3V identified as vaccine. BRSV was only identified by PCR testing. Interpretation of laboratory tests is appropriate as molecular based tests and virus isolation cannot separate field from vaccine strains. Additional testing using sequencing appears appropriate for identifying vaccine strains.
[Mh] Termos MeSH primário: Doenças dos Bovinos/diagnóstico
Doenças dos Bovinos/virologia
Infecções Respiratórias/veterinária
[Mh] Termos MeSH secundário: Animais
Bovinos
Coronavirus Bovino/isolamento & purificação
Vírus da Diarreia Viral Bovina Tipo 1/isolamento & purificação
Herpesvirus Bovino 1/isolamento & purificação
Nariz/virologia
Vírus da Parainfluenza 3 Bovina/isolamento & purificação
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Vírus Sincicial Respiratório Bovino/isolamento & purificação
Infecções Respiratórias/virologia
Estados Unidos
Vacinas Atenuadas
Vacinas Virais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Vaccines, Attenuated); 0 (Viral Vaccines)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160425
[St] Status:MEDLINE


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[PMID]:27073858
[Au] Autor:Walsh P; Behrens N; Carvallo Chaigneau FR; McEligot H; Agrawal K; Newman JW; Anderson M; Gershwin LJ
[Ad] Endereço:Department of Emergency Medicine, Division of Pediatric Emergency Medicine, Sutter Medical Center Sacramento, Sacramento, California, United States of America.
[Ti] Título:A Randomized Placebo Controlled Trial of Ibuprofen for Respiratory Syncytial Virus Infection in a Bovine Model.
[So] Source:PLoS One;11(4):e0152913, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Respiratory syncytial virus (RSV) is the most common cause of bronchiolitis and hospital admission in infants. An analogous disease occurs in cattle and costs US agriculture a billion dollars a year. RSV causes much of its morbidity indirectly via adverse effects of the host response to the virus. RSV is accompanied by elevated prostaglandin E2 (PGE2) which is followed by neutrophil led inflammation in the lung. Ibuprofen is a prototypical non-steroidal anti-inflammatory drug that decreases PGE2 levels by inhibiting cyclooxygenase. HYPOTHESES: We hypothesized that treatment of RSV with ibuprofen would decrease PGE2 levels, modulate the immune response, decrease clinical illness, and decrease the histopathological lung changes in a bovine model of RSV. We further hypothesized that viral replication would be unaffected. METHODS: We performed a randomized placebo controlled trial of ibuprofen in 16 outbred Holstein calves that we infected with RSV. We measured clinical scores, cyclooxygenase, lipoxygenase and endocannabinoid products in plasma and mediastinal lymph nodes and interleukin (Il)-4, Il-13, Il-17 and interferon-γ in mediastinal lymph nodes. RSV shedding was measured daily and nasal Il-6, Il-8 and Il-17 every other day. The calves were necropsied on Day 10 post inoculation and histology performed. RESULTS: One calf in the ibuprofen group required euthanasia on Day 8 of infection for respiratory distress. Clinical scores (p<0.01) and weight gain (p = 0.08) seemed better in the ibuprofen group. Ibuprofen decreased cyclooxygenase, lipoxygenase, and cytochrome P450 products, and increased monoacylglycerols in lung lymph nodes. Ibuprofen modulated the immune response as measured by narrowed range of observed Il-13, Il-17 and IFN-γ gene expression in mediastinal lymph nodes. Lung histology was not different between groups, and viral shedding was increased in calves randomized to ibuprofen. CONCLUSIONS: Ibuprofen decreased PGE2, modulated the immune response, and improved clinical outcomes. However lung histopathology was not affected and viral shedding was increased.
[Mh] Termos MeSH primário: Doenças dos Bovinos/tratamento farmacológico
Modelos Animais de Doenças
Ibuprofeno/uso terapêutico
Pulmão/efeitos dos fármacos
Infecções por Vírus Respiratório Sincicial/tratamento farmacológico
Vírus Sincicial Respiratório Bovino/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios não Esteroides/uso terapêutico
Bovinos
Doenças dos Bovinos/patologia
Doenças dos Bovinos/virologia
Citocinas/metabolismo
Técnicas Imunoenzimáticas
Pulmão/patologia
Pulmão/virologia
Masculino
Infecções por Vírus Respiratório Sincicial/patologia
Infecções por Vírus Respiratório Sincicial/virologia
Carga Viral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Cytokines); WK2XYI10QM (Ibuprofen)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160414
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0152913



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