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  1 / 1956 MEDLINE  
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[PMID]:29377949
[Au] Autor:Hibino A; Saito R; Taniguchi K; Zaraket H; Shobugawa Y; Matsui T; Suzuki H; Japanese HRSV Collaborative Study Group
[Ad] Endereço:Division of International Health (Public Health), Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
[Ti] Título:Molecular epidemiology of human respiratory syncytial virus among children in Japan during three seasons and hospitalization risk of genotype ON1.
[So] Source:PLoS One;13(1):e0192085, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We investigated the genetic diversity, the circulation patterns, and risk for hospital admission of human respiratory syncytial virus (HRSV) strains in Japan between 2012 through 2015. During the study period, 744 HRSV-positive cases were identified by rapid diagnostic test. Of these, 572 samples were positive by real-time PCR; 400 (69.9%) were HRSV-A, and 172 (30.1%) were HRSV-B. HRSV-A and -B alternated as the dominant strain in the subsequent seasons. Phylogenetic tree analysis of the second hyper-variable region of the G protein classified the HRSV-A specimens into NA1 (n = 242) and ON1 (n = 114) genotypes and the HRSV-B specimens into BA9 (n = 60), and BA10 (n = 27). The ON1 genotype, containing a 72-nucleotide duplication in the G protein's second hyper-variable region, was first detected in the 2012-2013 season but it predominated and replaced the older NA1 HRSV-A in the 2014-2015 season, which also coincided with a record number of HRSV cases reported to the National Infectious Disease Surveillance in Japan. The risk of hospitalization was 6.9 times higher for the ON1 genotype compared to NA1. In conclusion, our data showed that the emergence and predominance of the relatively new ON1 genotype in Japan was associated with a record high number of cases and increased risk for hospitalization.
[Mh] Termos MeSH primário: Hospitalização
Epidemiologia Molecular
Vírus Sincicial Respiratório Humano/genética
[Mh] Termos MeSH secundário: Genótipo
Seres Humanos
Japão
Reação em Cadeia da Polimerase em Tempo Real
Fatores de Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180130
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0192085


  2 / 1956 MEDLINE  
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[PMID]:29262797
[Au] Autor:Jiang W; Wu M; Zhou J; Wang Y; Hao C; Ji W; Zhang X; Gu W; Shao X
[Ad] Endereço:Department of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou, China.
[Ti] Título:Etiologic spectrum and occurrence of coinfections in children hospitalized with community-acquired pneumonia.
[So] Source:BMC Infect Dis;17(1):787, 2017 Dec 20.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Co-infections are common in childhood community acquired pneumonia (CAP). However, their etiological pattern and clinical impact remains inconclusive. METHODS: Eight hundred forty-six consecutive children with CAP were evaluated prospectively for the presence of viral and bacterial pathogens. Nasopharyngeal aspirates were examined by direct immunofluorescence assay or polymerase chain reaction (PCR) for viruses. PCR of nasopharyngeal aspirates and enzyme-linked immunosorbent assays were performed to detect M. pneumoniae. Bacteria was detected in blood, bronchoalveolar lavage specimen, or pleural fluid by culture. RESULTS: Causative pathogen was identified in 70.1% (593 of 846) of the patients. The most commonly detected pathogens were respiratory syncytial virus (RSV) (22.9%), human rhinovirus (HRV) (22.1%), M. pneumoniae (15.8%). Coinfection was identified in 34.6% (293 of 846) of the patients. The majority of these (209 [71.3%] of 293) were mixed viral-bacterial infections. Age < 6 months (odds ratio: 2.1; 95% confidence interval: 1.2-3.3) and admission of PICU (odds ratio: 12.5; 95% confidence interval: 1.6-97.4) were associated with mix infection. Patients with mix infection had a higher rate of PICU admission. CONCLUSIONS: The high mix infection burden in childhood CAP underscores a need for the enhancement of sensitive, inexpensive, and rapid diagnostics to accurately identify pneumonia pathogens.
[Mh] Termos MeSH primário: Coinfecção
Infecções Comunitárias Adquiridas
Pneumonia
[Mh] Termos MeSH secundário: Adolescente
Criança
Pré-Escolar
China/epidemiologia
Coinfecção/epidemiologia
Coinfecção/microbiologia
Coinfecção/virologia
Infecções Comunitárias Adquiridas/epidemiologia
Infecções Comunitárias Adquiridas/microbiologia
Infecções Comunitárias Adquiridas/virologia
Feminino
Seres Humanos
Lactente
Masculino
Mycoplasma pneumoniae
Pneumonia/epidemiologia
Pneumonia/microbiologia
Pneumonia/virologia
Vírus Sincicial Respiratório Humano
Rhinovirus
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2891-x


  3 / 1956 MEDLINE  
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[PMID]:29458556
[Au] Autor:Abdel-Moneim AS; Soliman MS; Kamel MM; El-Kholy AA
[Ad] Endereço:1​Microbiology Department, College of Medicine, Taif University, Al-Taif 21944, Saudi Arabia.
[Ti] Título:Sequence analysis of the G gene of hRSVA ON1 genotype from Egyptian children with acute respiratory tract infections.
[So] Source:J Med Microbiol;67(3):387-391, 2018 Mar.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Human respiratory syncytial virus causes severe lower respiratory tract infection in neonates and children. Genotype ON1, with duplication of 72-nt in the G gene, was first detected in Canada and then recorded in other countries. In the current study, we describe the first detection of the ON1 genotype among children in Egypt in 2014/2015. Sequence analysis of the full-attachment G gene revealed that the majority of the strains examined were related to the ON1 genotype and only one sample related to N1 genotype. The Egyptian ON1 strains showed unique non-silent mutations in addition to variable mutations near the antigenic sites in comparison to the original ON1 ancestor strain. Continuous surveillance of hRSV regionally and globally is needed to understand the evolutionary mechanisms and strategies adopted by hRSV and their inducers for better adaption to the host.
[Mh] Termos MeSH primário: Infecções por Vírus Respiratório Sincicial/virologia
Vírus Sincicial Respiratório Humano/genética
Infecções Respiratórias/virologia
Proteínas Virais de Fusão/genética
[Mh] Termos MeSH secundário: Canadá/epidemiologia
Pré-Escolar
Egito/epidemiologia
Evolução Molecular
Feminino
Genótipo
Seres Humanos
Lactente
Masculino
Mutação
Nasofaringe/virologia
Filogenia
Infecções por Vírus Respiratório Sincicial/epidemiologia
Vírus Sincicial Respiratório Humano/isolamento & purificação
Infecções Respiratórias/epidemiologia
Alinhamento de Sequência
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (G glycoprotein, Respiratory syncytial virus); 0 (Viral Fusion Proteins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000699


  4 / 1956 MEDLINE  
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[PMID]:29360861
[Au] Autor:Ogunsemowo O; Olaleye DO; Odaibo GN
[Ad] Endereço:Department of Virology, College of Medicine, University of Ibadan, Ibadan, Nigeria.
[Ti] Título:Genetic diversity of human respiratory syncytial virus circulating among children in Ibadan, Nigeria.
[So] Source:PLoS One;13(1):e0191494, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Human respiratory syncytial virus (HRSV) is the most common viral cause of acute lower respiratory tract infections (LRTIs) in infants and young children however, without an effective vaccine licensed for human use till date. Information on the circulating genotypes of HRSV from regions with high-burden of infection is vital in the global efforts towards the development of protective vaccine. We report here the genotypes of HRSV circulating among children in Ibadan, the first of such from Nigeria.Nasopharyngeal and oropharyngeal swabs collected from 231 children presenting with respiratory infections in some health facilities for care as well as those attending immunization centers for routine vaccination in Ibadan, Nigeria were used for the study. The 2nd hypervariable (HVR2) region of the glycoprotein (G) gene of HRSV was amplified and sequenced using HRSV group specific primers. HRSV was detected in 41 out of the 231 samples. Thirty-three of the isolates were successfully subtyped(22 subtype A and 11 subtype B). Fourteen of the subtype A and all the subtype B were successfully sequenced and genotyped. Phylogenetic analysis showed that genotype ON1 with 72 nucleotide (nt) duplication was the major subgroup A virus (11 of 14) detected together with genotype NA2. All the HRSV subtype B detected belong to the BA genotype with characteristic 60nt duplication. The ON1 genotypes vary considerably from the prototype strain due to amino acid substitutions including T292I which has not been reported elsewhere. The NA2 genotypes have mutations on four antigenic sites within the HVR2relative to the prototype A2. In conclusion, three genotypes of HRSV were found circulating in Ibadan, Nigeria. Additional study that will include isolates from other parts of the country will be done to determine the extent of genotype diversity of HRSV circulating in Nigeria.
[Mh] Termos MeSH primário: Variação Genética
Vírus Sincicial Respiratório Humano/genética
[Mh] Termos MeSH secundário: Criança
Pré-Escolar
Genes Virais
Genótipo
Seres Humanos
Nigéria
Filogenia
Reação em Cadeia da Polimerase
Vírus Sincicial Respiratório Humano/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191494


  5 / 1956 MEDLINE  
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[PMID]:29338045
[Au] Autor:Kim AR; Lee DH; Lee SH; Rubino I; Choi HJ; Quan FS
[Ad] Endereço:Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, Korea.
[Ti] Título:Protection induced by virus-like particle vaccine containing tandem repeat gene of respiratory syncytial virus G protein.
[So] Source:PLoS One;13(1):e0191277, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract illness in infants, young children and the elderly. However, there is no licensed vaccine available against RSV infection. In this study, we generated virus-like particle (VLP) vaccine and investigated the vaccine efficacy in a mouse model. For VLP vaccines, tandem gene (1-780 bp) for V1 VLPs and tandem repeat gene (repeated 450-780 bp) for V5 VLPs were constructed in pFastBacTM vectors, respectively. Influenza matrix protein 1 (M1) was used as a core protein in the VLPs. Notably, upon challenge infection, significantly lower virus loads were measured in the lung of mice immunized with V1 or V5 VLPs compared to those of naïve mice and formalin-inactivated RSV immunized control mice. In particular, V5 VLPs immunization showed significantly lower virus titers than V1 VLPs immunization. Furthermore, V5 VLPs immunization elicited increased memory B cells responses in the spleen. These results indicated that V5 VLP vaccine containing tandem repeat gene protein provided better protection than V1 VLPs with significantly decreased inflammation in the lungs. Thus, V5 VLPs could be a potential vaccine candidate against RSV.
[Mh] Termos MeSH primário: Infecções por Vírus Respiratório Sincicial/prevenção & controle
Vacinas contra Vírus Sincicial Respiratório/farmacologia
Vírus Sincicial Respiratório Humano/genética
Vírus Sincicial Respiratório Humano/imunologia
Vacinas de Partículas Semelhantes a Vírus/farmacologia
Proteínas do Envelope Viral/genética
Proteínas do Envelope Viral/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/sangue
Modelos Animais de Doenças
Feminino
Genes Virais
Seres Humanos
Pulmão/imunologia
Pulmão/patologia
Pulmão/virologia
Camundongos
Camundongos Endogâmicos BALB C
Infecções por Vírus Respiratório Sincicial/imunologia
Infecções por Vírus Respiratório Sincicial/virologia
Vacinas contra Vírus Sincicial Respiratório/genética
Sequências de Repetição em Tandem
Vacinas de Partículas Semelhantes a Vírus/genética
Carga Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Respiratory Syncytial Virus Vaccines); 0 (Vaccines, Virus-Like Particle); 0 (Viral Envelope Proteins); 0 (attachment protein G)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191277


  6 / 1956 MEDLINE  
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[PMID]:29346336
[Au] Autor:Rose EB; Wheatley A; Langley G; Gerber S; Haynes A
[Ti] Título:Respiratory Syncytial Virus Seasonality - United States, 2014-2017.
[So] Source:MMWR Morb Mortal Wkly Rep;67(2):71-76, 2018 Jan 19.
[Is] ISSN:1545-861X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract infection in young children worldwide (1-3). In the United States, RSV infection results in >57,000 hospitalizations and 2 million outpatient visits each year among children aged <5 years (3). Recent studies have highlighted the importance of RSV in adults as well as children (4). CDC reported RSV seasonality nationally, by U.S. Department of Health and Human Services (HHS) regions* and for the state of Florida, using a new statistical method that analyzes polymerase chain reaction (PCR) laboratory detections reported to the National Respiratory and Enteric Virus Surveillance System (NREVSS) (https://www.cdc.gov/surveillance/nrevss/index.html). Nationally, across three RSV seasons, lasting from the week ending July 5, 2014 through July 1, 2017, the median RSV onset occurred at week 41 (mid-October), and lasted 31 weeks until week 18 (early May). The median national peak occurred at week 5 (early February). Using these new methods, RSV season circulation patterns differed from those reported from previous seasons (5). Health care providers and public health officials use RSV circulation data to guide diagnostic testing and to time the administration of RSV immunoprophylaxis for populations at high risk for severe respiratory illness (6). With several vaccines and other immunoprophlyaxis products in development, estimates of RSV circulation are also important to the design of clinical trials and future vaccine effectiveness studies.
[Mh] Termos MeSH primário: Vigilância da População
Infecções por Vírus Respiratório Sincicial/epidemiologia
Vírus Sincicial Respiratório Humano/isolamento & purificação
[Mh] Termos MeSH secundário: Pré-Escolar
Seres Humanos
Incidência
Lactente
Infecções por Vírus Respiratório Sincicial/diagnóstico
Estações do Ano
Estados Unidos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180121
[Lr] Data última revisão:
180121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180119
[St] Status:MEDLINE
[do] DOI:10.15585/mmwr.mm6702a4


  7 / 1956 MEDLINE  
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[PMID]:29190684
[Au] Autor:Pale M; Nacoto A; Tivane A; Nguenha N; Machalele L; Gundane F; Muteto D; Chilundo J; Mavale S; Semá-Baltazar C; Pires G; Augusto O; Mussá T; Gudo E
[Ad] Endereço:Department of Technologic Platforms, National Institute of Health, Ministry of Health, Maputo, Mozambique.
[Ti] Título:Respiratory syncytial and influenza viruses in children under 2 years old with severe acute respiratory infection (SARI) in Maputo, 2015.
[So] Source:PLoS One;12(11):e0186735, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Although respiratory syncytial virus (RSV) and influenza virus (influenza) infections are one of the leading causes of Severe Acute Respiratory Infections (SARI) and death in young children worldwide, little is known about the burden of these pathogens in Mozambique. MATERIAL AND METHODS: From January 2015 to January 2016, nasopharyngeal swabs from 450 children, aged ≤2 years, who had been admitted to the Pediatric Department of the Maputo Central Hospital (HCM) in Mozambique, suffering with SARI were enrolled and tested for influenza and RSV using a real-time PCR assay. RESULTS: Influenza and RSV were detected in 2.4% (11/450) and 26.7% (113/424) of the participants. Children with influenza were slightly older than those infected with RSV (10 months in influenza-infected children compared to 3 months in RSV-infected children); male children were predominant in both groups (63.6% versus 54.9% in children with influenza and RSV, respectively). There was a trend towards a higher frequency of influenza (72.7%) and RSV (93.8%) cases in the dry season. Bronchopneumonia, bronchitis and respiratory distress were the most common diagnoses at admission. Antibiotics were administered to 27,3% and 15,9% of the children with influenza and RSV, respectively. Two children, of whom, one was positive for RSV (aged 6 months) and another was positive for Influenza (aged 3 months) died; both were children of HIV seropositive mothers and had bronchopneumonia. CONCLUSIONS: Our data demonstrated that RSV, and less frequently influenza, occurs in children with SARI in urban/sub-urban settings from southern Mozambique. The occurrence of deaths in small children suspected of being HIV-infected, suggests that particular attention should be given to this vulnerable population. Our data also provide evidence of antibiotics prescription in children with respiratory viral infection, which represents an important public health problem and calls for urgent interventions.
[Mh] Termos MeSH primário: Influenza Humana/epidemiologia
Orthomyxoviridae/isolamento & purificação
Infecções por Vírus Respiratório Sincicial/epidemiologia
Vírus Sincicial Respiratório Humano/isolamento & purificação
[Mh] Termos MeSH secundário: Pré-Escolar
Feminino
Seres Humanos
Lactente
Recém-Nascido
Masculino
Moçambique/epidemiologia
Orthomyxoviridae/genética
Vigilância da População
Reação em Cadeia da Polimerase em Tempo Real
Vírus Sincicial Respiratório Humano/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186735


  8 / 1956 MEDLINE  
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[PMID]:28468888
[Au] Autor:Leemans A; De Schryver M; Van der Gucht W; Heykers A; Pintelon I; Hotard AL; Moore ML; Melero JA; McLellan JS; Graham BS; Broadbent L; Power UF; Caljon G; Cos P; Maes L; Delputte P
[Ad] Endereço:Laboratory of Microbiology, Parasitology and Hygiene, University of Antwerp, Antwerp, Belgium.
[Ti] Título:Antibody-Induced Internalization of the Human Respiratory Syncytial Virus Fusion Protein.
[So] Source:J Virol;91(14), 2017 Jul 15.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Respiratory syncytial virus (RSV) infections remain a major cause of respiratory disease and hospitalizations among infants. Infection recurs frequently and establishes a weak and short-lived immunity. To date, RSV immunoprophylaxis and vaccine research is mainly focused on the RSV fusion (F) protein, but a vaccine remains elusive. The RSV F protein is a highly conserved surface glycoprotein and is the main target of neutralizing antibodies induced by natural infection. Here, we analyzed an internalization process of antigen-antibody complexes after binding of RSV-specific antibodies to RSV antigens expressed on the surface of infected cells. The RSV F protein and attachment (G) protein were found to be internalized in both infected and transfected cells after the addition of either RSV-specific polyclonal antibodies (PAbs) or RSV glycoprotein-specific monoclonal antibodies (MAbs), as determined by indirect immunofluorescence staining and flow-cytometric analysis. Internalization experiments with different cell lines, well-differentiated primary bronchial epithelial cells (WD-PBECs), and RSV isolates suggest that antibody internalization can be considered a general feature of RSV. More specifically for RSV F, the mechanism of internalization was shown to be clathrin dependent. All RSV F-targeted MAbs tested, regardless of their epitopes, induced internalization of RSV F. No differences could be observed between the different MAbs, indicating that RSV F internalization was epitope independent. Since this process can be either antiviral, by affecting virus assembly and production, or beneficial for the virus, by limiting the efficacy of antibodies and effector mechanism, further research is required to determine the extent to which this occurs and how this might impact RSV replication. Current research into the development of new immunoprophylaxis and vaccines is mainly focused on the RSV F protein since, among others, RSV F-specific antibodies are able to protect infants from severe disease, if administered prophylactically. However, antibody responses established after natural RSV infections are poorly protective against reinfection, and high levels of antibodies do not always correlate with protection. Therefore, RSV might be capable of interfering, at least partially, with antibody-induced neutralization. In this study, a process through which surface-expressed RSV F proteins are internalized after interaction with RSV-specific antibodies is described. One the one hand, this antigen-antibody complex internalization could result in an antiviral effect, since it may interfere with virus particle formation and virus production. On the other hand, this mechanism may also reduce the efficacy of antibody-mediated effector mechanisms toward infected cells.
[Mh] Termos MeSH primário: Anticorpos Antivirais/metabolismo
Endocitose
Vírus Sincicial Respiratório Humano/imunologia
Proteínas Virais de Fusão/metabolismo
[Mh] Termos MeSH secundário: Complexo Antígeno-Anticorpo/metabolismo
Células Cultivadas
Citometria de Fluxo
Técnica Indireta de Fluorescência para Anticorpo
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Antigen-Antibody Complex); 0 (Viral Fusion Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE


  9 / 1956 MEDLINE  
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[PMID]:29028839
[Au] Autor:Ward C; Maselko M; Lupfer C; Prescott M; Pastey MK
[Ad] Endereço:Department of Veterinary Biomedical Sciences, Oregon State University, Corvallis, Oregon, United States of America.
[Ti] Título:Interaction of the Human Respiratory Syncytial Virus matrix protein with cellular adaptor protein complex 3 plays a critical role in trafficking.
[So] Source:PLoS One;12(10):e0184629, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Human Respiratory Syncytial Virus (HRSV) is a leading cause of bronchopneumonia in infants and the elderly. To date, knowledge of viral and host protein interactions within HRSV is limited and are critical areas of research. Here, we show that HRSV Matrix (M) protein interacts with the cellular adaptor protein complex 3 specifically via its medium subunit (AP-3Mu3A). This novel protein-protein interaction was first detected via yeast-two hybrid screen and was further confirmed in a mammalian system by immunofluorescence colocalization and co-immunoprecipitation. This novel interaction is further substantiated by the presence of a known tyrosine-based adaptor protein MU subunit sorting signal sequence, YXXФ: where Ф is a bulky hydrophobic residue, which is conserved across the related RSV M proteins. Analysis of point-mutated HRSV M derivatives indicated that AP-3Mu3A- mediated trafficking is contingent on the presence of the tyrosine residue within the YXXL sorting sequence at amino acids 197-200 of the M protein. AP-3Mu3A is up regulated at 24 hours post-infection in infected cells versus mock-infected HEp2 cells. Together, our data suggests that the AP-3 complex plays a critical role in the trafficking of HRSV proteins specifically matrix in epithelial cells. The results of this study add new insights and targets that may lead to the development of potential antivirals and attenuating mutations suitable for candidate vaccines in the future.
[Mh] Termos MeSH primário: Complexo 3 de Proteínas Adaptadoras/metabolismo
Vírus Sincicial Respiratório Humano/metabolismo
Proteínas da Matriz Viral/metabolismo
[Mh] Termos MeSH secundário: Complexo 3 de Proteínas Adaptadoras/química
Motivos de Aminoácidos
Sequência de Aminoácidos
Sequência Conservada
Células HeLa
Seres Humanos
Ligação Proteica
Estabilidade Proteica
Subunidades Proteicas/química
Subunidades Proteicas/metabolismo
Transporte Proteico
Vírus Sincicial Respiratório Humano/fisiologia
Regulação para Cima
Proteínas da Matriz Viral/química
Montagem de Vírus
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adaptor Protein Complex 3); 0 (Protein Subunits); 0 (Viral Matrix Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171014
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184629


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[PMID]:28953993
[Au] Autor:Souza C; Zanchin NI; Krieger MA; Ludwig A
[Ad] Endereço:Fundação Oswaldo Cruz-Fiocruz, Instituto Carlos Chagas, Laboratório de Genômica Funcional, Curitiba, PR, Brasil.
[Ti] Título:In silico analysis of amino acid variation in human respiratory syncytial virus: insights into immunodiagnostics.
[So] Source:Mem Inst Oswaldo Cruz;112(10):655-663, 2017 Oct.
[Is] ISSN:1678-8060
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The highly contagious nature of human respiratory syncytial virus (HRSV) and the gravity of its infection in newborns and vulnerable adults pose a serious public health problem. Thus, a rapid and sensitive diagnostic test for viral detection that can be implemented upon the first appearance of symptoms is needed. The genetic variation of the virus must be considered for immunodiagnostic purposes. OBJECTIVES: To analyse HRSV genetic variation and discuss the possible consequences for capture immunoassay development. METHODS: We performed a wide analysis of N, F and G protein variation based on the HRSV sequences currently available in the GenBank database. We also evaluated their similarity with homologous proteins from other viruses. FINDINGS: The mean amino acid divergences for the N, F, and G proteins between HRSV-A and HRSV-B were determined to be approximately 4%, 10% and 47%, respectively. Due to their high conservation, assays based on the full-length N and F proteins may not distinguish HRSV from human metapneumovirus and other Mononegavirales viruses, and the full-length G protein would most likely produce false negative results due to its high divergence. MAIN CONCLUSIONS: We have identified specific regions in each of these three proteins that have higher potential to produce specific results, and their combined utilisation should be considered for immunoassay development.
[Mh] Termos MeSH primário: Variação Genética
Infecções por Vírus Respiratório Sincicial/virologia
Vírus Sincicial Respiratório Humano/genética
Proteínas Virais/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Genótipo
Seres Humanos
Testes Imunológicos
Filogenia
Infecções por Vírus Respiratório Sincicial/diagnóstico
Vírus Sincicial Respiratório Humano/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170928
[St] Status:MEDLINE



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