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[PMID]:29256424
[Au] Autor:Bigarré L; Plassiart G; de Boisséson C; Pallandre L; Pozet F; Ledoré Y; Fontaine P; Lieffrig F
[Ad] Endereço:ANSES, Laboratoire Ploufragan-Plouzané, 29280 Plouzané, France.
[Ti] Título:Molecular investigations of outbreaks of Perch perhabdovirus infections in pike-perch.
[So] Source:Dis Aquat Organ;127(1):19-27, 2017 Dec 19.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In 2016, a total of 5 massive mortality episodes each affecting hundreds of thousands of pike-perch Sander lucioperca larvae occurred at 2 sites in 2 Western European countries. For each episode, perhabdoviruses related to the perch rhabdovirus (PRV) were detected in samples, using either PCR or cell culture combined with PCR. The sequences of the glycoprotein (g), phosphoprotein (p) and nucleoprotein (n) genes of these samples demonstrated that 2 different genotypes were present at 1 site, each associated with 1 of the 3 episodes. At the other site, a single genotype was associated with the 2 outbreaks. Furthermore, this genotype was strictly identical to 1 genotype involved in the outbreaks of the first site, strongly suggesting a common origin for these 2 viruses. The common origin was confirmed a posteriori because some larvae introduced to both sites had exactly the same geographic origin in Eastern Europe. Taken together, the molecular and epidemiological data suggest that both horizontal and vertical transmission of 2 distinct strains of perhabdoviruses were involved in the various outbreaks affecting pike-perch.
[Mh] Termos MeSH primário: Surtos de Doenças/veterinária
Doenças dos Peixes/virologia
Perciformes/virologia
Infecções por Rhabdoviridae/veterinária
Rhabdoviridae
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Europa (Continente)/epidemiologia
Doenças dos Peixes/epidemiologia
Larva/virologia
Rhabdoviridae/genética
Infecções por Rhabdoviridae/epidemiologia
Infecções por Rhabdoviridae/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.3354/dao03177


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[PMID]:27773769
[Au] Autor:Dietzgen RG; Kondo H; Goodin MM; Kurath G; Vasilakis N
[Ad] Endereço:Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, Queensland 4072, Australia. Electronic address: r.dietzgen@uq.edu.au.
[Ti] Título:The family Rhabdoviridae: mono- and bipartite negative-sense RNA viruses with diverse genome organization and common evolutionary origins.
[So] Source:Virus Res;227:158-170, 2017 01 02.
[Is] ISSN:1872-7492
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The family Rhabdoviridae consists of mostly enveloped, bullet-shaped or bacilliform viruses with a negative-sense, single-stranded RNA genome that infect vertebrates, invertebrates or plants. This ecological diversity is reflected by the diversity and complexity of their genomes. Five canonical structural protein genes are conserved in all rhabdoviruses, but may be overprinted, overlapped or interspersed with several novel and diverse accessory genes. This review gives an overview of the characteristics and diversity of rhabdoviruses, their taxonomic classification, replication mechanism, properties of classical rhabdoviruses such as rabies virus and rhabdoviruses with complex genomes, rhabdoviruses infecting aquatic species, and plant rhabdoviruses with both mono- and bipartite genomes.
[Mh] Termos MeSH primário: Evolução Molecular
Variação Genética
Genoma Viral
RNA Viral
Rhabdoviridae/classificação
Rhabdoviridae/fisiologia
[Mh] Termos MeSH secundário: Animais
Código de Barras de DNA Taxonômico
Interações Hospedeiro-Patógeno
Filogenia
Vírus de Plantas/classificação
Vírus de Plantas/fisiologia
Biossíntese de Proteínas
Vírus de RNA/classificação
Vírus de RNA/fisiologia
Transcrição Genética
Vertebrados
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE


  3 / 929 MEDLINE  
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[PMID]:28461211
[Au] Autor:Luo K; Li Y; Xia L; Hu W; Gao W; Guo L; Tian G; Qi Z; Yuan H; Xu Q
[Ad] Endereço:Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Jingzhou 434020, China.
[Ti] Título:Analysis of the expression patterns of the novel large multigene TRIM gene family (finTRIM) in zebrafish.
[So] Source:Fish Shellfish Immunol;66:224-230, 2017 Jul.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tripartite motif (TRIM) proteins are receiving increased research interest because of their roles in a wide range of cellular biological processes in innate immunity. In zebrafish (Danio rerio), the functions of the finTRIM (ftr) family are unclear. In the present study, we investigated the expression pattern of ftr12, ftr51, ftr67, ftr82, ftr83, and ftr84 in zebrafish for the first time. The results showed that ftr12, ftr67, and ftr84 are maternally expressed in the oocyte and highly expressed at the early stage (0-4 hpf) of embryo (P < 0.05), suggesting their involvement in the embryonic innate defense system. The ftr82 gene was highly expressed at 8 hpf (P < 0.05), which implied that the embryos could synthesize their own immunity-related mRNAs. However, ftr51 and ftr83 were highest at 8 hpf (2.33 and 51.53 relative to ß-actin respectively) and might mediate embryonic development. The expression levels of ftr12, ftr51, and ftr67 were highest in the gill, intestines, and liver, respectively. Ftr82, ftr83, and ftr84 were predominantly expressed in the kidney, suggesting that these finTRIMs might play roles in both immunity and non-immunity-related tissue compartments. Zebrafish embryonic fibroblast (ZF4) cells were infected with Grass carp reovirus (GCRV) and Spring viremia of carp virus (SVCV). During GCRV infection, the expression of ftr12 was significantly upregulated from 12 h to 24 h; and ftr51 and ftr67 increased from 3 h to 12 h. The expressions of ftr82, ftr83, and ftr84 were only upregulated at 12 h, 12 h, and 24 h, respectively. All of these genes were significantly downregulated at 48 h (P < 0.05). Challenge with SVCV upregulated the expressions of ftr12 and ftr51 at 12 h and 48 h (P < 0.05), respectively, and ftr67 reached its highest expression level at 3 h. ftr82 showed only a slight upregulation at 6 h and 48 h, and ftr83 and ftr84 were consecutively increased, reaching their highest levels at 12 h (P < 0.05). Meanwhile, ftr67 and ftr83 were significantly downregulated at 48 h (P < 0.05). Our research demonstrated that ftr12, ftr51, ftr67, ftr82, ftr83, and ftr84 probably have important roles in innate immune responses and in non-immunity-related tissues.
[Mh] Termos MeSH primário: Doenças dos Peixes/genética
Expressão Gênica
Imunidade Inata/genética
Família Multigênica
Proteínas com Motivo Tripartido/genética
Proteínas de Peixe-Zebra/genética
Peixe-Zebra
[Mh] Termos MeSH secundário: Animais
Doenças dos Peixes/imunologia
Doenças dos Peixes/virologia
Expressão Gênica/imunologia
Perfilação da Expressão Gênica/veterinária
Reoviridae/fisiologia
Infecções por Reoviridae/genética
Infecções por Reoviridae/imunologia
Infecções por Reoviridae/veterinária
Rhabdoviridae/fisiologia
Infecções por Rhabdoviridae/genética
Infecções por Rhabdoviridae/imunologia
Infecções por Rhabdoviridae/veterinária
Análise de Sequência de DNA/veterinária
Proteínas com Motivo Tripartido/metabolismo
Proteínas de Peixe-Zebra/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Tripartite Motif Proteins); 0 (Zebrafish Proteins)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171215
[Lr] Data última revisão:
171215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


  4 / 929 MEDLINE  
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[PMID]:29024851
[Au] Autor:Maghodia AB; Jarvis DL
[Ad] Endereço:GlycoBac, LLC, Laramie, WY 82072, USA.
[Ti] Título:Infectivity of Sf-rhabdovirus variants in insect and mammalian cell lines.
[So] Source:Virology;512:234-245, 2017 Dec.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sf-rhabdovirus was only recently identified as an adventitious agent of Spodoptera frugiperda (Sf) cell lines used as hosts for baculovirus vectors. As such, we still know little about its genetic variation, infectivity, and the potential impact of variation on the Sf-rhabdovirus-host interaction. Here, we characterized Sf-rhabdoviruses from two widely used Sf cell lines to confirm and extend information on Sf-rhabdovirus variation. We then used our novel Sf-rhabdovirus-negative (Sf-RVN) Sf cell line to assess the infectivity of variants with and without a 320bp X/L deletion and found both established productive persistent infections in Sf-RVN cells. We also assessed their infectivity using heterologous insect and mammalian cell lines and found neither established productive persistent infections in these cells. These results are the first to directly demonstrate Sf-rhabdoviruses are infectious for Sf cells, irrespective of the X/L deletion. They also confirm and extend previous results indicating Sf-rhabdoviruses have a narrow host range.
[Mh] Termos MeSH primário: Insetos
Mamíferos
Rhabdoviridae/classificação
Rhabdoviridae/genética
[Mh] Termos MeSH secundário: Animais
Bovinos
Linhagem Celular
Regulação Viral da Expressão Gênica
Células HeLa
Especificidade de Hospedeiro
Seres Humanos
Rim/citologia
Rim/virologia
Rhabdoviridae/fisiologia
Proteínas Virais/genética
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE


  5 / 929 MEDLINE  
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[PMID]:28915434
[Au] Autor:Bello-Perez M; Falco A; Medina-Gali R; Pereiro P; Encinar JA; Novoa B; Perez L; Coll J
[Ad] Endereço:Instituto de Biología Molecular y Celular, Universidad Miguel Hernández (IBMC-UMH). Elche, Spain. Electronic address: melissa.bello@goumh.umh.es.
[Ti] Título:Neutralization of viral infectivity by zebrafish c-reactive protein isoforms.
[So] Source:Mol Immunol;91:145-155, 2017 11.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This work explores the unexpected in vivo and in vitro anti-viral functions of the seven c-reactive protein (crp1-7) genes of zebrafish (Danio rerio). First results showed heterogeneous crp1-7 transcript levels in healthy wild-type zebrafish tissues and organs and how those levels heterogeneously changed not only after bacterial but also after viral infections, including those in adaptive immunity-deficient rag1 mutants. As shown by microarray hybridization and proteomic techniques, crp2/CRP2 and crp5/CRP5 transcripts/proteins were among the most modulated during in vivo viral infection situations including the highest responses in the absence of adaptive immunity. In contrast crp1/CRP1/and crp7/CRP7 very often remained unmodulated. All evidences suggested that zebrafish crp2-6/CRP2-6 may have in vivo anti-viral activities in addition to their well known anti-bacterial and/or physiological functions in mammalians. Confirming those expectations, in vitro neutralization and in vivo protection against spring viremia carp virus (SVCV) infections were demonstrated by crp2-6/CRP2-6 using crp1-7 transfected and/or CRP1-7-enriched supernatant-treated fish cells and crp2-5-injected one-cell stage embryo eggs, respectively. All these findings discovered a crp1-7/CRP1-7 primitive anti-viral functional diversity.These findings may help to study similar functions on the one-gene-coded human CRP, which is widely used as a clinical biomarker for bacterial infections, tissue inflammation and coronary heart diseases.
[Mh] Termos MeSH primário: Proteína C-Reativa
Doenças dos Peixes
Rhabdoviridae/imunologia
Peixe-Zebra
[Mh] Termos MeSH secundário: Animais
Proteína C-Reativa/genética
Proteína C-Reativa/imunologia
Doenças dos Peixes/genética
Doenças dos Peixes/imunologia
Doenças dos Peixes/virologia
Proteínas de Homeodomínio/genética
Proteínas de Homeodomínio/imunologia
Mutação
Isoformas de Proteínas/genética
Isoformas de Proteínas/imunologia
Peixe-Zebra/genética
Peixe-Zebra/imunologia
Peixe-Zebra/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Homeodomain Proteins); 0 (Protein Isoforms); 128559-51-3 (RAG-1 protein); 9007-41-4 (C-Reactive Protein)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE


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[PMID]:28768857
[Au] Autor:Nakagawa K; Kobayashi Y; Ito N; Suzuki Y; Okada K; Makino M; Goto H; Takahashi T; Sugiyama M
[Ad] Endereço:The United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.
[Ti] Título:Molecular Function Analysis of Rabies Virus RNA Polymerase L Protein by Using an L Gene-Deficient Virus.
[So] Source:J Virol;91(20), 2017 Oct 15.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:While the RNA-dependent RNA polymerase L protein of rabies virus (RABV), a member of the genus of the family , has potential to be a therapeutic target for rabies, the molecular functions of this protein have remained largely unknown. In this study, to obtain a novel experimental tool for molecular function analysis of the RABV L protein, we established by using a reverse genetics approach an L gene-deficient RABV (Nishi-ΔL/Nluc), which infects, propagates, and correspondingly produces NanoLuc luciferase in cultured neuroblastoma cells transfected to express the L protein. -Complementation with wild-type L protein, but not that with a functionally defective L protein mutant, efficiently supported luciferase production by Nishi-ΔL/Nluc, confirming its potential for function analysis of the L protein. Based on the findings obtained from comprehensive genetic analyses of L genes from various RABV and other lyssavirus species, we examined the functional importance of a highly conserved L protein region at positions 1914 to 1933 by a -complementation assay with Nishi-ΔL/Nluc and a series of L protein mutants. The results revealed that the amino acid sequence at positions 1929 to 1933 (NPYNE) is functionally important, and this was supported by other findings that this sequence is critical for binding of the L protein with its essential cofactor, P protein, and thus also for L protein's RNA polymerase activity. Our findings provide useful information for the development of an anti-RABV drug targeting the L-P protein interaction. To the best of our knowledge, this is the first report on the establishment of an L gene-deficient, reporter gene-expressing virus in all species of the order , also highlighting its applicability to a -complementation assay, which is useful for molecular function analyses of their L proteins. Moreover, this study revealed for the first time that the NPYNE sequence at positions 1929 to 1933 in the RABV L protein is important for L protein's interaction with the P protein, consistent with and extending the results of a previous study showing that the P protein-binding domain in the L protein is located in its C-terminal region, at positions 1562 to 2127. This study indicates that the NPYNE sequence is a promising target for the development of an inhibitor of viral RNA synthesis, which has high potential as a therapeutic drug for rabies.
[Mh] Termos MeSH primário: RNA Polimerases Dirigidas por DNA/genética
RNA Polimerases Dirigidas por DNA/metabolismo
Genes Virais
Vírus da Raiva/enzimologia
Proteínas Virais/genética
Proteínas Virais/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
RNA Polimerases Dirigidas por DNA/química
Teste de Complementação Genética
Luciferases/biossíntese
Luciferases/genética
Lyssavirus/genética
Mutação
Fosfoproteínas/metabolismo
RNA Viral/genética
Vírus da Raiva/genética
Genética Reversa
Rhabdoviridae/genética
Proteínas Virais/química
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phosphoproteins); 0 (RNA, Viral); 0 (Viral Proteins); EC 1.13.12.- (Luciferases); EC 2.7.7.48 (L protein, Rabies virus); EC 2.7.7.6 (DNA-Directed RNA Polymerases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE


  7 / 929 MEDLINE  
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[PMID]:28635588
[Au] Autor:Jang C; Wang R; Wells J; Leon F; Farman M; Hammond J; Goodin MM
[Ad] Endereço:1​Department of Plant Pathology, University of Kentucky, Lexington, KY, USA.
[Ti] Título:Genome sequence variation in the constricta strain dramatically alters the protein interaction and localization map of Potato yellow dwarf virus.
[So] Source:J Gen Virol;98(6):1526-1536, 2017 Jun.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The genome sequence of the constricta strain of Potato yellow dwarf virus (CYDV) was determined to be 12 792 nt long and organized into seven ORFs with the gene order 3'-N-X-P-Y-M-G-L-5', which encodes the nucleocapsid, phospho, movement, matrix, glyco, and RNA-dependent RNA polymerase proteins, respectively, except for X, which is of unknown function. Cloned ORFs for each gene, except L, were used to construct a protein interaction and localization map (PILM) for this virus, which shares greater than 80 % amino acid similarity in all ORFs except X and P with the sanguinolenta strain of this species (SYDV). Protein localization patterns and interactions unique to each viral strain were identified, resulting in strain-specific PILMs. Localization of CYDV and SYDV proteins in virus-infected cells mapped subcellular loci likely to be sites of replication, morphogenesis and movement.
[Mh] Termos MeSH primário: Variação Genética
Interações Hospedeiro-Patógeno
Rhabdoviridae/genética
Rhabdoviridae/fisiologia
Proteínas Virais/análise
Proteínas Virais/genética
[Mh] Termos MeSH secundário: Capsicum/virologia
Ordem dos Genes
Genoma Viral
Lycopersicon esculentum/virologia
Microscopia Confocal
Fases de Leitura Aberta
Análise de Sequência de DNA
Solanum tuberosum/virologia
Tabaco/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171115
[Lr] Data última revisão:
171115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170622
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000771


  8 / 929 MEDLINE  
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[PMID]:28618303
[Au] Autor:Martin KM; Barandoc-Alviar K; Schneweis DJ; Stewart CL; Rotenberg D; Whitfield AE
[Ad] Endereço:Department of Plant Pathology, Kansas State University, Manhattan, KS, USA.
[Ti] Título:Transcriptomic response of the insect vector, Peregrinus maidis, to Maize mosaic rhabdovirus and identification of conserved responses to propagative viruses in hopper vectors.
[So] Source:Virology;509:71-81, 2017 Sep.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Maize mosaic virus (MMV) is a plant-pathogenic rhabdovirus that is transmitted by the corn planthopper, Peregrinus maidis, in a propagative manner. P. maidis supports long-term MMV infections with no negative effects on insect performance. To elucidate whole-body transcriptome responses to virus infection, RNA-Seq was used to examine differential gene expression of virus-infected adult insects, and libraries were prepared from replicated groups of virus-exposed insects and non-exposed insects. From the 68,003 de novo-assembled transcripts, 144 were differentially-expressed (DE) during viral infection with comparable numbers up- and down-regulated. DE transcripts with similarity to genes associated with transposable elements (i.e., RNA-directed DNA polymerases) were enriched and may represent a mechanisim for modulating virus infection. Comparison of the P. maidis DE transcripts to published propagative virus-responsive transcript databases for two other hopper vectors revealed that 16% of the DE transcripts were shared across the three systems and may represent conserved responses to propagative viruses.
[Mh] Termos MeSH primário: Perfilação da Expressão Gênica
Hemípteros/genética
Hemípteros/virologia
Insetos Vetores/genética
Insetos Vetores/virologia
Rhabdoviridae/imunologia
[Mh] Termos MeSH secundário: Animais
Zea mays/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170721
[Lr] Data última revisão:
170721
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170616
[St] Status:MEDLINE


  9 / 929 MEDLINE  
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[PMID]:28568891
[Au] Autor:Le Boeuf F; Selman M; Son HH; Bergeron A; Chen A; Tsang J; Butterwick D; Arulanandam R; Forbes NE; Tzelepis F; Bell JC; Werier J; Abdelbary H; Diallo JS
[Ad] Endereço:Centre for Innovative Cancer research, Ottawa Hospital Research Institute, Ottawa, ON, KlH 8L6, Canada.
[Ti] Título:Oncolytic Maraba Virus MG1 as a Treatment for Sarcoma.
[So] Source:Int J Cancer;141(6):1257-1264, 2017 Sep 15.
[Is] ISSN:1097-0215
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The poor prognosis of patients with advanced bone and soft-tissue sarcoma has not changed in the past several decades, highlighting the necessity for new therapeutic approaches. Immunotherapies, including oncolytic viral (OV) therapy, have shown great promise in a number of clinical trials for a variety of tumor types. However, the effective application of OV in treating sarcoma still remains to be demonstrated. Although few pre-clinical studies using distinct OVs have been performed and demonstrated therapeutic benefit in sarcoma models, a side-by-side comparison of clinically relevant OV platforms has not been performed. Four clinically relevant OV platforms (Reovirus, Vaccinia virus, Herpes-simplex virus and Rhabdovirus) were screened for their ability to infect and kill human and canine sarcoma cell lines in vitro, and human sarcoma specimens ex vivo. In vivo treatment efficacy was tested in a murine model. The rhabdovirus MG1 demonstrated the highest potency in vitro. Ex vivo, MG1 productively infected more than 80% of human sarcoma tissues tested, and treatment in vivo led to a significant increase in long-lasting cures in sarcoma-bearing mice. Importantly, MG1 treatment induced the generation of memory immune response that provided protection against a subsequent tumor challenge. This study opens the door for the use of MG1-based oncolytic immunotherapy strategies as treatment for sarcoma or as a component of a combined therapy.
[Mh] Termos MeSH primário: Terapia Viral Oncolítica/métodos
Rhabdoviridae/fisiologia
Sarcoma/terapia
Sarcoma/virologia
[Mh] Termos MeSH secundário: Animais
Neoplasias Ósseas/terapia
Neoplasias Ósseas/virologia
Linhagem Celular Tumoral
Cães
Feminino
Seres Humanos
Camundongos
Camundongos Endogâmicos BALB C
Osteossarcoma/terapia
Osteossarcoma/virologia
Sarcoma de Ewing/terapia
Sarcoma de Ewing/virologia
Sarcoma Sinovial/terapia
Sarcoma Sinovial/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170602
[St] Status:MEDLINE
[do] DOI:10.1002/ijc.30813


  10 / 929 MEDLINE  
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[PMID]:28478311
[Au] Autor:Kondo H; Hirota K; Maruyama K; Andika IB; Suzuki N
[Ad] Endereço:Institute of Plant Science and Resources (IPSR), Okayama University, Kurashiki 710-0046, Japan. Electronic address: hkondo@okayama-u.ac.jp.
[Ti] Título:A possible occurrence of genome reassortment among bipartite rhabdoviruses.
[So] Source:Virology;508:18-25, 2017 Aug.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Orchid fleck virus (OFV) represents a rhabdovirus with a unique bipartite genome. OFV genetic diversity at the whole genome level has not been described. Using the partial genome sequence of RNA1, we have determined that several OFV isolates derived from orchids in Japan belong to two genetically distant subgroups: subgroup I, the members of which are distributed worldwide but previously not known in Asia, and subgroup II, which is commonly distributed in Japan. However, complete genome sequence analysis of a novel Japanese subgroup I isolate revealed that although its RNA1 sequence differs considerably from those of subgroup II isolates, its RNA2 sequence is almost identical to them. Based on phylogenetic and recombination analyses, the genome reassortment events were predicted to occur between OFV subgroups including other unseen strains. Our data show that genome reassortment contributes to the genetic diversities of the bipartite rhabdoviruses and its occurrence may be geographically constrained.
[Mh] Termos MeSH primário: Genoma Viral
Orchidaceae/virologia
Doenças das Plantas/virologia
Vírus Reordenados/genética
Rhabdoviridae/genética
[Mh] Termos MeSH secundário: Ásia
Fases de Leitura Aberta
Filogenia
Vírus Reordenados/classificação
Vírus Reordenados/fisiologia
Recombinação Genética
Rhabdoviridae/classificação
Rhabdoviridae/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170508
[St] Status:MEDLINE



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