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  1 / 397 MEDLINE  
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[PMID]:29256423
[Au] Autor:Ito T; Olesen NJ
[Ad] Endereço:Tamaki Laboratory, Research Centre for Fish Diseases, National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency, 224-1 Hiruda, Tamaki, Mie 519-0423, Japan.
[Ti] Título:Viral haemorrhagic septicaemia virus (VHSV) remains viable for several days but at low levels in the water flea Moina macrocopa.
[So] Source:Dis Aquat Organ;127(1):11-18, 2017 Dec 19.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Viral haemorrhagic septicaemia virus (VHSV) Genotype IVb has been isolated from amphipods belonging to the genus Diporeia, but it has yet to be established whether crustacean zooplankton act as vectors of this virus for fish species. Therefore, we evaluated the viability of infectious VHSV in the water flea Moina macrocopa. VHSV was re-isolated from replicate groups of M. macrocopa that had been immersed with 108.0, 107.0, and 105.0 TCID50 ml-1 of VHSV (DK-3592B, Genotype Ia). Furthermore, 40 M. macrocopa that had been immersed with 108.0 TCID50 ml-1 of VHSV for 72 h had VHSV titers of 102.7-104.3 TCID50. Thus, VHSV was clearly taken up by M. macrocopa and remained viable in this crustacean for several days. However, no mortality was observed over a 28 d period in rainbow trout Oncorhynchus mykiss that were fed VHSV-contaminated M. macrocopa for 14 d, and we found that the virus titer significantly decreased after a 4 h incubation with pyloric caecal extracts from rainbow trout, indicating that passage through the gut is likely to result in a significant decrease in viral titer. This may explain why consumption of prey containing low levels of VHSV did not result in clinical VHS.
[Mh] Termos MeSH primário: Cladóceros/virologia
Septicemia Hemorrágica Viral/virologia
Novirhabdovirus/fisiologia
Oncorhynchus mykiss
[Mh] Termos MeSH secundário: Animais
Reservatórios de Doenças
Conteúdo Gastrointestinal/virologia
Septicemia Hemorrágica Viral/transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.3354/dao03185


  2 / 397 MEDLINE  
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[PMID]:28640747
[Au] Autor:Baillon L; Mérour E; Cabon J; Louboutin L; Quenault H; Touzain F; Morin T; Blanchard Y; Biacchesi S; Brémont M
[Ad] Endereço:1​VIM, INRA, Université Paris-Saclay, Jouy-en-Josas, France.
[Ti] Título:A single amino acid change in the non-structural NV protein impacts the virulence phenotype of Viral hemorrhagic septicemia virus in trout.
[So] Source:J Gen Virol;98(6):1181-1184, 2017 Jun.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Novirhabdoviruses like the Viral hemorrhagic septicemia virus (VHSV) are rhabdoviruses infecting fish. In the current study, RNA genomes of different VHSV field isolates classified as high, medium or low virulent phenotypes have been sequenced by next-generation sequencing and compared. Various amino acid changes, depending on the VHSV phenotype, have been identified in all the VHSV proteins. As a starting point, we focused our study on the non-virion (NV) non-structural protein in which an arginine residue (R116) is present in all the virulent isolates and replaced by a serine/asparagine residue S/N116 in the attenuated isolates. A recombinant virus derived from a virulent VHSV strain in which the NV R116 residue has been replaced by a serine, rVHSVNVR116S, was generated by reverse genetics and used to infect juvenile trout. We showed that rVHSVNVR116S was highly attenuated and that surviving fish were almost completely protected from a challenge with the wild-type VHSV.
[Mh] Termos MeSH primário: Substituição de Aminoácidos
Doenças dos Peixes/patologia
Doenças dos Peixes/virologia
Novirhabdovirus/patogenicidade
Infecções por Rhabdoviridae/veterinária
Proteínas Virais/genética
Fatores de Virulência/genética
[Mh] Termos MeSH secundário: Animais
Genoma Viral
Novirhabdovirus/genética
Novirhabdovirus/isolamento & purificação
Fenótipo
Genética Reversa
Infecções por Rhabdoviridae/patologia
Infecções por Rhabdoviridae/virologia
Análise de Sequência de DNA
Truta
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (NV protein, Rhabdovirus); 0 (Viral Proteins); 0 (Virulence Factors)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170717
[Lr] Data última revisão:
170717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000830


  3 / 397 MEDLINE  
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[PMID]:28388934
[Au] Autor:Zhang J; Tang X; Sheng X; Xing J; Zhan W
[Ad] Endereço:Laboratory of Pathology and Immunology of Aquatic Animals, Ocean University of China, No.5 Yushan Road, Qingdao, 266003, China.
[Ti] Título:Isolation and identification of a new strain of hirame rhabdovirus (HIRRV) from Japanese flounder Paralichthys olivaceus in China.
[So] Source:Virol J;14(1):73, 2017 Apr 07.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Hirame rhabdovirus virus (HIRRV) is a rhabdovirus that causes acute hemorrhage disease in fish culture, resulting in a great economic loss in parts of Asia and Europe. METHODS: In this study, we isolated a virus strain named as CNPo2015 from cultured Japanese flounder in Shandong province, China. Cell isolation, electron microscopic observation, RT-PCR detection and phylogenetic analysis were used for virus identification. Further, artificial infection experiment was conducted for virulence testing. RESULTS: The gross signs included abdominal distension, fin reddening and yellow ascitic fluid in the abdominal cavity. Histopathological examination revealed marked cell degeneration and necrosis in the kidney. The tissue homogenates induced obvious cytopathic effects in EPC, FHM and FG cell lines. Electron microscopic observation showed the virus had a bullet-like shape with a capsule membrane. RT-PCR and sequencing analysis revealed that CNPo2015 belonged to the HIRRV with high sequence identity to HIRRV isolates. Infection experiment confirmed that the HIRRV CNPo2015 strain was virulent to flounder juveniles with a LD value of 1.0 × 10 TCID /fish. CONCLUSION: In conclusion, we described the first isolation and characterization of a HIRRV from Japanese flounder in China. This will provide a candidate material for further research on the infection mechanism and preventive strategies of HIRRV.
[Mh] Termos MeSH primário: Doenças dos Peixes/virologia
Linguado/virologia
Novirhabdovirus/genética
Novirhabdovirus/isolamento & purificação
Infecções por Rhabdoviridae/veterinária
[Mh] Termos MeSH secundário: Animais
Aquicultura
China
Microscopia Eletrônica
Novirhabdovirus/patogenicidade
Novirhabdovirus/ultraestrutura
Filogenia
RNA Viral/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Infecções por Rhabdoviridae/virologia
Análise de Sequência de DNA
Vírion/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170823
[Lr] Data última revisão:
170823
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170409
[St] Status:MEDLINE
[do] DOI:10.1186/s12985-017-0742-4


  4 / 397 MEDLINE  
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[PMID]:28302579
[Au] Autor:Soleto I; Abós B; Castro R; González L; Tafalla C; Granja AG
[Ad] Endereço:Centro de Investigación en Sanidad Animal (CISA-INIA), Madrid, Spain.
[Ti] Título:The BAFF / APRIL axis plays an important role in virus-induced peritoneal responses in rainbow trout.
[So] Source:Fish Shellfish Immunol;64:210-217, 2017 May.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:IgM B cells have been recently demonstrated to be key regulators of peritoneal inflammation in teleost, as a large number of them occupy the peritoneal cavity after 48 h of antigenic stimulation. Despite this, the number of studies addressing the mechanism through which this cell population expands and differentiates in response to stimuli has been scarcely addressed. Because the BAFF/APRIL axis is known to play a major role in B cell survival and differentiation in mammals, we hypothesized that it could be affected in the peritoneal cavity in response to an inflammatory stimulus. To verify this hypothesis, we studied how BAFF, APRIL and the fish-specific related cytokine BALM as well as their putative receptors are regulated in rainbow trout after intraperitoneal (i.p.) injection of viral hemorrhagic septicemia virus (VHSV). When the transcriptional analysis was performed in total cells from the peritoneum, we observed that VHSV provoked an up-regulation of both BAFF and BAFF receptor (BAFF-R) mRNA levels. However, when we examined how isolated peritoneal IgM B cells were transcriptionally affected by VHSV i.p. injection, we found that APRIL, BALM and the transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI) were also up-regulated in response to the virus. IgM cells, on the other hand, only up-regulated BALM transcription in response to VHSV. Finally, to gain further insight on the role that these cytokines play in the peritoneum, we have studied their effect on the survival of peritoneal IgM B cells. This work demonstrates a key role for the BAFF/APRIL axis in the peritoneal inflammatory response and contributes to further understanding how IgM B cells are regulated at this specific peripheral site.
[Mh] Termos MeSH primário: Doenças dos Peixes/genética
Proteínas de Peixes/genética
Novirhabdovirus/fisiologia
Oncorhynchus mykiss
Infecções por Rhabdoviridae/veterinária
[Mh] Termos MeSH secundário: Animais
Receptor do Fator Ativador de Células B/genética
Receptor do Fator Ativador de Células B/metabolismo
Linfócitos B/imunologia
Citocinas/genética
Citocinas/metabolismo
Doenças dos Peixes/imunologia
Doenças dos Peixes/virologia
Proteínas de Peixes/metabolismo
Peritônio/fisiopatologia
Peritônio/virologia
Infecções por Rhabdoviridae/genética
Infecções por Rhabdoviridae/imunologia
Infecções por Rhabdoviridae/virologia
Membro 13 da Superfamília de Ligantes de Fatores de Necrose Tumoral/genética
Membro 13 da Superfamília de Ligantes de Fatores de Necrose Tumoral/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (B-Cell Activation Factor Receptor); 0 (Cytokines); 0 (Fish Proteins); 0 (Tumor Necrosis Factor Ligand Superfamily Member 13)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170318
[St] Status:MEDLINE


  5 / 397 MEDLINE  
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[PMID]:28192199
[Au] Autor:Tien NQ; Kim TJ; Kim TG
[Ad] Endereço:Department of Bioactive Material Sciences, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeollabuk-do 54896, Republic of Korea.
[Ti] Título:Viral hemorrhagic septicemia virus glycoprotein production in tobacco.
[So] Source:Protein Expr Purif;133:170-176, 2017 May.
[Is] ISSN:1096-0279
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Viral hemorrhagic septicemia virus (VHSV) causes mortality in numerous marine and freshwater fish species resulting in heavy losses in fish farming. The glycoprotein gene of VHSV was fused with the cholera toxin B subunit (CTB) and expressed transiently in leaf tissues of Nicotiana benthamiana via the agroinfiltration method. The glycoprotein gene was divided into two parts to improve assembly of CTB fusion proteins (CTB-VHSV and CTB-VHSV ). Production of CTB fusion proteins was confirmed in the agroinfiltrated leaf tissue by western blot analysis. The plant-produced CTB fusion proteins showed biological activity to G -ganglioside, a receptor for biologically active CTB, on G -ELISA. The expression level of the CTB-VHSV fusion proteins was 0.86% (CTB-VHSV ) and 0.93% (CTB-VHSV ) of total proteins in agroinfiltrated leaf tissue, as determined by G -ELISA. These results suggest that Agrobacterium-mediated transient expression of CTB fusion antigens of VHSV is a rapid and convenient method and demonstrate the feasibility of using agroinfiltrated plant leaf tissues expressing CTB-fusion antigens as a plant-based vaccine to prevent VHSV infection.
[Mh] Termos MeSH primário: Glicoproteínas
Novirhabdovirus/genética
Folhas de Planta/metabolismo
Plantas Geneticamente Modificadas/metabolismo
Tabaco/metabolismo
Proteínas Virais
[Mh] Termos MeSH secundário: Toxina da Cólera/biossíntese
Toxina da Cólera/genética
Glicoproteínas/biossíntese
Glicoproteínas/genética
Novirhabdovirus/metabolismo
Folhas de Planta/genética
Plantas Geneticamente Modificadas/genética
Proteínas Recombinantes de Fusão/biossíntese
Proteínas Recombinantes de Fusão/genética
Tabaco/genética
Proteínas Virais/biossíntese
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycoproteins); 0 (Recombinant Fusion Proteins); 0 (Viral Proteins); 9012-63-9 (Cholera Toxin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170717
[Lr] Data última revisão:
170717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170214
[St] Status:MEDLINE


  6 / 397 MEDLINE  
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[PMID]:28190196
[Au] Autor:Park YJ; Moon C; Kang JH; Choi TJ
[Ad] Endereço:Department of Microbiology, Pukyong National University, 45, Yongso-ro, Nam-Gu, Busan, 48513, Republic of Korea.
[Ti] Título:Antiviral effects of extracts from Celosia cristata and Raphanus sativus roots against viral hemorrhagic septicemia virus.
[So] Source:Arch Virol;162(6):1711-1716, 2017 Jun.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:The antiviral activity of an extract mixture from Celosia cristata and Raphanus sativus was tested against viral hemorrhagic septicemia virus (VHSV). Pretreatment of EPC cells with this extract up to 72 h before VHSV infection markedly reduced the virus titer, but it had no effect when added after virus inoculation. In olive flounder that received 5 µg of extract per fish, Mx expression peaked at 48 h after treatment. In contrast, ISG15 and TLR2 expression peaked at 72 h, and that of TLR7 peaked at 48 h, followed by a slight decrease at 72 h, indicating that the antiviral activity was mediated by induction of gene expression involved in the innate immune response.
[Mh] Termos MeSH primário: Antivirais/farmacologia
Celosia/química
Doenças dos Peixes/virologia
Septicemia Hemorrágica Viral/virologia
Novirhabdovirus/efeitos dos fármacos
Extratos Vegetais/farmacologia
Raphanus/química
[Mh] Termos MeSH secundário: Animais
Antivirais/isolamento & purificação
Doenças dos Peixes/genética
Doenças dos Peixes/metabolismo
Proteínas de Peixes/genética
Proteínas de Peixes/metabolismo
Linguado/virologia
Septicemia Hemorrágica Viral/genética
Septicemia Hemorrágica Viral/metabolismo
Novirhabdovirus/fisiologia
Extratos Vegetais/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Fish Proteins); 0 (Plant Extracts)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170630
[Lr] Data última revisão:
170630
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170213
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3270-z


  7 / 397 MEDLINE  
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[PMID]:28155193
[Au] Autor:Kim MS; Lee SJ; Choi SH; Kang YJ; Kim KH
[Ad] Endereço:Graduate School of Integrated Bioindustry, Sejong University, Seoul, 05006, South Korea.
[Ti] Título:Dexamethasone treatment decreases replication of viral hemorrhagic septicemia virus in Epithelioma papulosum cyprini cells.
[So] Source:Arch Virol;162(5):1387-1392, 2017 May.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:The expression of Mx1 in EPC cells after treatment with poly(I:C) or infection with viral hemorrhagic septicemia virus (VHSV) was significantly suppressed by treatment with dexamethasone. However, the titer of VHSV did not increase but instead decreased after dexamethasone treatment. This suggests that dexamethasone not only downregulates type I IFN but also affects certain factors that are necessary for VHSV replication. An important effect of HSP90 on replication of RNA viruses and downregulation of HSP90 by glucocorticoids have been reported. In this study, dexamethasone downregulated HSP90α expression in EPC cells that were stimulated with poly(I:C) or infected with VHSV. Furthermore, cells treated with an HSP90 inhibitor, geldanamycin, showed significantly decreased titers of VHSV, suggesting that HSP90 may be an important host component involved in VHSV replication, and HSP90 inhibition might be one of the causes for the observed reduction in viral titer caused by dexamethasone treatment.
[Mh] Termos MeSH primário: Cyprinidae/virologia
Dexametasona/farmacologia
Doenças dos Peixes/virologia
Proteínas de Choque Térmico HSP90/biossíntese
Proteínas de Resistência a Myxovirus/biossíntese
Novirhabdovirus/genética
Poli I-C/farmacologia
[Mh] Termos MeSH secundário: Animais
Benzoquinonas/farmacologia
Carcinoma/metabolismo
Carcinoma/virologia
Linhagem Celular Tumoral
Replicação do DNA/efeitos dos fármacos
Regulação para Baixo
Proteínas de Choque Térmico HSP90/antagonistas & inibidores
Imunossupressão
Interferon Tipo I/biossíntese
Lactamas Macrocíclicas/farmacologia
Novirhabdovirus/imunologia
Replicação Viral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzoquinones); 0 (HSP90 Heat-Shock Proteins); 0 (Interferon Type I); 0 (Lactams, Macrocyclic); 0 (Myxovirus Resistance Proteins); 7S5I7G3JQL (Dexamethasone); O84C90HH2L (Poly I-C); Z3K3VJ16KU (geldanamycin)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170204
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3248-x


  8 / 397 MEDLINE  
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[PMID]:28126619
[Au] Autor:Lazarte JM; Kim YR; Lee JS; Im SP; Kim SW; Jung JW; Kim J; Lee WJ; Jung TS
[Ad] Endereço:Laboratory of Aquatic Animal Diseases, College of Veterinary Medicine, Gyeongsang National University, Jinju, South Korea.
[Ti] Título:Enhancement of glycoprotein-based DNA vaccine for viral hemorrhagic septicemia virus (VHSV) via addition of the molecular adjuvant, DDX41.
[So] Source:Fish Shellfish Immunol;62:356-365, 2017 Mar.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The use of molecular adjuvants to improve the immunogenicity of DNA vaccines has been thoroughly studied in recent years. Glycoprotein (G)-based DNA vaccines had been proven to be effective in combating infection against Rhabdovirus (especially infectious hematopoietic necrosis virus, IHNV) in salmonids. DDX41 is a helicase known to induce antiviral and inflammatory responses by inducing a type I IFN innate immune response. To gain more information regarding G-based DNA vaccines in olive flounder (Paralicthys olivaceus), we tried to develop a more efficient G-based DNA vaccine by adding a molecular adjuvant, DDX41. We designed a DNA vaccine in which the VHSV glycoprotein (G-protein) and DDX41 were driven by the EF-1α and CMV promoters, respectively. Olive flounders were intramuscularly immunized with 1 µg of plasmids encoding the G-based DNA vaccine alone (pEF-G), the molecular adjuvant alone (pEF-D), or the vaccine-adjuvant construct (pEF-GD). At two different time points, 15 and 30 days later, the fish were intraperitoneally infected with VHSV (100 µL; 1 × 10 TCID /mL). Our assays revealed that the plasmid constructs showed up-regulated expression of IFN-1 and its associated genes at day 3 post-vaccination in both kidney and spleen samples. Specifically, pEF-GD showed statistically higher expression of immune response genes than pEF-G and pEF-D treated group (p < 0.05/p < 0.001). After VHSV challenge, the fish group treated with pEF-GD showed higher survival rate than the pEF-G treated group, though difference was not statistically significant in the 15 dpv challenged group however in the 30 dpv challenged group, the difference was statistically significant (p < 0.05). Together, these results clearly demonstrate that DDX41 is an effective adjuvant for the G-based DNA vaccine in olive flounder. Our novel findings could facilitate the development of more effective DNA vaccines for the aquaculture industry.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/farmacologia
RNA Helicases DEAD-box/farmacologia
Proteínas de Peixes/farmacologia
Linguados
Septicemia Hemorrágica Viral/prevenção & controle
Novirhabdovirus/imunologia
Proteínas Virais/imunologia
Vacinas Virais/imunologia
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos/metabolismo
Animais
Glicoproteínas/imunologia
Septicemia Hemorrágica Viral/virologia
Imunidade/efeitos dos fármacos
Vacinas de DNA/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Fish Proteins); 0 (Glycoproteins); 0 (Vaccines, DNA); 0 (Viral Proteins); 0 (Viral Vaccines); EC 3.6.4.13 (DEAD-box RNA Helicases)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE


  9 / 397 MEDLINE  
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[PMID]:28108340
[Au] Autor:Hwang JY; Kwon MG; Jung SH; Park MA; Kim DW; Cho WS; Park JW; Son MH
[Ad] Endereço:Aquatic Disease Control Division, National Institute of Fisheries Science, 216 GijangHaean-Ro, Gijang-up, Gijang-Gun, Busan 46083, Republic of Korea. Electronic address: jinihwang@korea.kr.
[Ti] Título:RNA-Seq transcriptome analysis of the olive flounder (Paralichthys olivaceus) kidney response to vaccination with heat-inactivated viral hemorrhagic septicemia virus.
[So] Source:Fish Shellfish Immunol;62:221-226, 2017 Mar.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Viral hemorrhagic septicemia (VHS) is a highly contagious disease of cultured flounder caused by VHS virus (VHSV). To develop effective VHSV vaccines, it is essential to understand the molecular mechanisms underlying the host's protective response against VHSV. The purpose of this study is to clarify which genes are involved in the protective response of olive flounder after VHSV vaccination. We first injected olive flounder intraperitoneally with 10 TCID heat-inactivated VHSV vaccine and evaluated the vaccine efficacy at 20 °C. Fish vaccinated with heat-inactivated VHSV were significantly protected compared to non-vaccinated fish, with a relative percentage survival of 83%. To analyze the vaccination-induced changes in the expression profiles of genes, kidneys were collected from control and vaccinated fish at days 1, 3, and 7 after vaccination and global gene expression profiling was carried out by RNA sequencing. The analysis revealed that 15,001 genes were differentially expressed by at least 2-fold between vaccinated fish and non-vaccinated controls. Of these, 58 genes clustered into the acute phase response, Toll-like receptor, interferon-inducible/regulatory proteins, and apoptosis pathways. These data provided insights into the molecular mechanisms underlying the protective immune response of olive flounder against heat-inactivated VHSV vaccine and might aid future studies to develop a highly immunogenic vaccine against VHSV in flounder.
[Mh] Termos MeSH primário: Linguados
Novirhabdovirus/imunologia
Transcriptoma
Vacinação/veterinária
Vacinas Virais/imunologia
[Mh] Termos MeSH secundário: Animais
Doenças dos Peixes/imunologia
Doenças dos Peixes/prevenção & controle
Perfilação da Expressão Gênica/veterinária
Septicemia Hemorrágica Viral/imunologia
Septicemia Hemorrágica Viral/prevenção & controle
Sequenciamento de Nucleotídeos em Larga Escala/veterinária
Injeções Intraperitoneais/veterinária
Vacinas de Produtos Inativados/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Vaccines, Inactivated); 0 (Viral Vaccines)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170122
[St] Status:MEDLINE


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[PMID]:28007485
[Au] Autor:Najib A; Kim MS; Kim KH
[Ad] Endereço:Department of Aquatic Life Medicine, Pukyong National University, Busan 48513, Republic of Korea.
[Ti] Título:Viral hemorrhagic septicemia virus (VHSV) infection-mediated sequential changes in microRNAs profile of Epithelioma papulosum cyprini (EPC) cells.
[So] Source:Fish Shellfish Immunol;61:93-99, 2017 Feb.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:MicroRNAs are small non-coding RNAs and are involved in the regulation of wide biological processes. Viral hemorrhagic septicemia virus (VHSV) is the causative agent of viral hemorrhagic septicemia (VHS) disease causing a heavy loss in aquaculture farms. In this study, we tried to explore the effect of VHSV infection on microRNAs profile of Epithelioma papulosum cyprini (EPC) cells at different points of time (0, 3, 12, 24, and 48 h post infection). A total of 355 conserved microRNAs and 3 novel microRNAs were identified, and among them, 103 microRNAs were differentially expressed. The number of differentially expressed microRNAs was highly increased at 24 h.p.i compared to 3 h.p.i and 12 h.p.i., suggesting that EPC cells might not actively respond to VHSV infection at an early infection period, which can allow viruses to transcript and translate their genes enough to produce viral particles that can infect to another cells. Among the differentially expressed microRNAs, 2 miRNAs (miR-735 and miR-738) that were reported only in fish species were highly upregulated, and based on the target prediction, they could regulate several immune pathways. Furthermore, the present results showed the upregulation of representative immune regulating microRNAs such as miR-146a, miR-155, and miR-99. The target prediction of differentially expressed miRNAs, GO, and KEGG pathways analysis revealed that several biological processes and different pathways were affected by the viral infection. The present dynamical changing patterns of differentially expressed microRNAs in response to the progression of VHSV infection suggest that microRNA profile that was analyzed at one time point cannot provide enough information for the interpretation of the disease mechanism. Considering the wide and complex interactions between microRNAs and genes expression, the present results provide the basis for the understanding of VHSV infection-mediated cellular responses and for future investigations on the development of possible control measures.
[Mh] Termos MeSH primário: Cyprinidae
Doenças dos Peixes/genética
Expressão Gênica
Septicemia Hemorrágica Viral/genética
MicroRNAs/genética
Novirhabdovirus/fisiologia
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Tumoral
Doenças dos Peixes/metabolismo
Doenças dos Peixes/virologia
Septicemia Hemorrágica Viral/metabolismo
Septicemia Hemorrágica Viral/virologia
MicroRNAs/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161224
[St] Status:MEDLINE



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