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  1 / 1706 MEDLINE  
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[PMID]:28902913
[Au] Autor:Li Y; Khalafalla AI; Paden CR; Yusof MF; Eltahir YM; Al Hammadi ZM; Tao Y; Queen K; Hosani FA; Gerber SI; Hall AJ; Al Muhairi S; Tong S
[Ad] Endereço:Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.
[Ti] Título:Identification of diverse viruses in upper respiratory samples in dromedary camels from United Arab Emirates.
[So] Source:PLoS One;12(9):e0184718, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Camels are known carriers for many viral pathogens, including Middle East respiratory syndrome coronavirus (MERS-CoV). It is likely that there are additional, as yet unidentified viruses in camels with the potential to cause disease in humans. In this study, we performed metagenomic sequencing analysis on nasopharyngeal swab samples from 108 MERS-CoV-positive dromedary camels from a live animal market in Abu Dhabi, United Arab Emirates. We obtained a total of 846.72 million high-quality reads from these nasopharyngeal swab samples, of which 2.88 million (0.34%) were related to viral sequences while 512.63 million (60.5%) and 50.87 million (6%) matched bacterial and eukaryotic sequences, respectively. Among the viral reads, sequences related to mammalian viruses from 13 genera in 10 viral families were identified, including Coronaviridae, Nairoviridae, Paramyxoviridae, Parvoviridae, Polyomaviridae, Papillomaviridae, Astroviridae, Picornaviridae, Poxviridae, and Genomoviridae. Some viral sequences belong to known camel or human viruses and others are from potentially novel camel viruses with only limited sequence similarity to virus sequences in GenBank. A total of five potentially novel virus species or strains were identified. Co-infection of at least two recently identified camel coronaviruses was detected in 92.6% of the camels in the study. This study provides a comprehensive survey of viruses in the virome of upper respiratory samples in camels that have extensive contact with the human population.
[Mh] Termos MeSH primário: Camelus/virologia
Viroses/veterinária
Zoonoses/virologia
[Mh] Termos MeSH secundário: Animais
Coronaviridae/classificação
Coronaviridae/genética
Coronaviridae/isolamento & purificação
Seres Humanos
Metagenômica
Filogenia
Análise de Sequência de DNA
Emirados Árabes Unidos/epidemiologia
Viroses/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184718


  2 / 1706 MEDLINE  
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[PMID]:28850669
[Au] Autor:Lei J; Hilgenfeld R
[Ad] Endereço:Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Germany.
[Ti] Título:RNA-virus proteases counteracting host innate immunity.
[So] Source:FEBS Lett;591(20):3190-3210, 2017 Oct.
[Is] ISSN:1873-3468
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Virus invasion triggers host immune responses, in particular, innate immune responses. Pathogen-associated molecular patterns of viruses (such as dsRNA, ssRNA, or viral proteins) released during virus replication are detected by the corresponding pattern-recognition receptors of the host, and innate immune responses are induced. Through production of type-I and type-III interferons as well as various other cytokines, the host innate immune system forms the frontline to protect host cells and inhibit virus infection. Not surprisingly, viruses have evolved diverse strategies to counter this antiviral system. In this review, we discuss the multiple strategies used by proteases of positive-sense single-stranded RNA viruses of the families Picornaviridae, Coronaviridae, and Flaviviridae, when counteracting host innate immune responses.
[Mh] Termos MeSH primário: Evasão da Resposta Imune
Imunidade Inata
Interferons/imunologia
Receptores Toll-Like/imunologia
Proteínas Virais/imunologia
Viroses/imunologia
[Mh] Termos MeSH secundário: Animais
Coronaviridae/genética
Coronaviridae/imunologia
Citocinas/genética
Citocinas/imunologia
Flaviviridae/genética
Flaviviridae/imunologia
Regulação da Expressão Gênica
Seres Humanos
Interferons/genética
Picornaviridae/genética
Picornaviridae/imunologia
Estrutura Secundária de Proteína
Transdução de Sinais
Receptores Toll-Like/genética
Proteínas Virais/química
Proteínas Virais/genética
Viroses/genética
Viroses/patologia
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Cytokines); 0 (Toll-Like Receptors); 0 (Viral Proteins); 9008-11-1 (Interferons)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE
[do] DOI:10.1002/1873-3468.12827


  3 / 1706 MEDLINE  
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[PMID]:28220326
[Au] Autor:Noh JY; Yoon SW; Kim DJ; Lee MS; Kim JH; Na W; Song D; Jeong DG; Kim HK
[Ad] Endereço:Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
[Ti] Título:Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR.
[So] Source:Arch Virol;162(6):1617-1623, 2017 Jun.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Since severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) coronaviruses (CoVs) share similar characteristics with respect to clinical signs, etiology, and transmission, methods for a rapid and accurate differential diagnosis are important. Therefore, the aim of this study was to develop a duplex real-time reverse transcription (RT)-PCR method for the simultaneous detection of these viruses. Primers and probes that target the conserved spike S2 region of human SARS-CoV, MERS-CoV, and their related bat CoVs were designed. The results of real-time RT-PCR showed specific reactions for each virus with adequate detection limits of 50-100 copies/mL and 5-100 copies/mL using pUC57-SARS-pS2 (a template for SARS-CoV) and pGEM-MERS-S2 (a template for MERS-CoV), respectively. In addition, this real-time RT-PCR system was able to detect the target viruses SARS-like bat CoV and MERS-CoV in bat fecal samples and sputum of MERS patients, respectively. Therefore, this newly developed real-time RT-PCR method is expected to detect not only SARS-CoV and MERS-CoV in humans but also several bat CoVs that are closely related to these viruses in bats.
[Mh] Termos MeSH primário: Coronaviridae/isolamento & purificação
Coronavírus da Síndrome Respiratória do Oriente Médio/isolamento & purificação
Reação em Cadeia da Polimerase em Tempo Real/métodos
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
Vírus da SARS/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Quirópteros/virologia
Coronaviridae/genética
Infecções por Coronavirus/virologia
Diagnóstico Diferencial
Fezes/virologia
Seres Humanos
Limite de Detecção
Coronavírus da Síndrome Respiratória do Oriente Médio/genética
RNA Viral/genética
Vírus da SARS/genética
Alinhamento de Sequência
Síndrome Respiratória Aguda Grave/virologia
Escarro/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170720
[Lr] Data última revisão:
170720
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170222
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3281-9


  4 / 1706 MEDLINE  
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[PMID]:28158275
[Au] Autor:Kindler E; Gil-Cruz C; Spanier J; Li Y; Wilhelm J; Rabouw HH; Züst R; Hwang M; V'kovski P; Stalder H; Marti S; Habjan M; Cervantes-Barragan L; Elliot R; Karl N; Gaughan C; van Kuppeveld FJ; Silverman RH; Keller M; Ludewig B; Bergmann CC; Ziebuhr J; Weiss SR; Kalinke U; Thiel V
[Ad] Endereço:Department of Infectious Diseases and Pathobiology, University of Bern, Bern, Switzerland.
[Ti] Título:Early endonuclease-mediated evasion of RNA sensing ensures efficient coronavirus replication.
[So] Source:PLoS Pathog;13(2):e1006195, 2017 Feb.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Coronaviruses are of veterinary and medical importance and include highly pathogenic zoonotic viruses, such as SARS-CoV and MERS-CoV. They are known to efficiently evade early innate immune responses, manifesting in almost negligible expression of type-I interferons (IFN-I). This evasion strategy suggests an evolutionary conserved viral function that has evolved to prevent RNA-based sensing of infection in vertebrate hosts. Here we show that the coronavirus endonuclease (EndoU) activity is key to prevent early induction of double-stranded RNA (dsRNA) host cell responses. Replication of EndoU-deficient coronaviruses is greatly attenuated in vivo and severely restricted in primary cells even during the early phase of the infection. In macrophages we found immediate induction of IFN-I expression and RNase L-mediated breakdown of ribosomal RNA. Accordingly, EndoU-deficient viruses can retain replication only in cells that are deficient in IFN-I expression or sensing, and in cells lacking both RNase L and PKR. Collectively our results demonstrate that the coronavirus EndoU efficiently prevents simultaneous activation of host cell dsRNA sensors, such as Mda5, OAS and PKR. The localization of the EndoU activity at the site of viral RNA synthesis-within the replicase complex-suggests that coronaviruses have evolved a viral RNA decay pathway to evade early innate and intrinsic antiviral host cell responses.
[Mh] Termos MeSH primário: Coronaviridae/enzimologia
Infecções por Coronavirus/imunologia
Endonucleases/imunologia
Evasão da Resposta Imune/fisiologia
Proteínas Virais/imunologia
[Mh] Termos MeSH secundário: Animais
Coronaviridae/imunologia
Ensaio de Imunoadsorção Enzimática
Citometria de Fluxo
Interações Hospedeiro-Patógeno/imunologia
Seres Humanos
Imunidade Inata/imunologia
Camundongos
Camundongos Endogâmicos C57BL
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins); EC 3.1.- (Endonucleases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170623
[Lr] Data última revisão:
170623
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170204
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006195


  5 / 1706 MEDLINE  
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[PMID]:27984784
[Au] Autor:Zhu X; Fang L; Wang D; Yang Y; Chen J; Ye X; Foda MF; Xiao S
[Ad] Endereço:State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
[Ti] Título:Porcine deltacoronavirus nsp5 inhibits interferon-ß production through the cleavage of NEMO.
[So] Source:Virology;502:33-38, 2017 Feb.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Porcine deltacoronavirus (PDCoV) causes acute enteric disease and mortality in seronegative neonatal piglets. Previously we have demonstrated that PDCoV infection suppresses the production of interferon-beta (IFN-ß), while the detailed mechanisms are poorly understood. Here, we demonstrate that nonstructural protein 5 (nsp5) of PDCoV, the 3C-like protease, significantly inhibits Sendai virus (SEV)-induced IFN-ß production by targeting the NF-κB essential modulator (NEMO), confirmed by the diminished function of NEMO cleaved by PDCoV. The PDCoV nsp5 cleavage site in the NEMO protein was identified as glutamine 231, and was identical to the porcine epidemic diarrhea virus nsp5 cleavage site, revealing the likelihood of a common target in NEMO for coronaviruses. Furthermore, this cleavage impaired the ability of NEMO to activate the IFN response and downstream signaling. Taken together, our findings reveal PDCoV nsp5 to be a newly identified IFN antagonist and enhance the understanding of immune evasion by deltacoronaviruses.
[Mh] Termos MeSH primário: Infecções por Coronaviridae/veterinária
Coronaviridae/enzimologia
Cisteína Endopeptidases/metabolismo
Quinase I-kappa B/metabolismo
Interferon beta/metabolismo
Doenças dos Suínos/enzimologia
Proteínas não Estruturais Virais/metabolismo
[Mh] Termos MeSH secundário: Animais
Coronaviridae/genética
Infecções por Coronaviridae/enzimologia
Infecções por Coronaviridae/metabolismo
Infecções por Coronaviridae/virologia
Cisteína Endopeptidases/genética
Interações Hospedeiro-Patógeno
Quinase I-kappa B/genética
Interferon beta/genética
Processamento de Proteína Pós-Traducional
Suínos
Doenças dos Suínos/genética
Doenças dos Suínos/metabolismo
Doenças dos Suínos/virologia
Proteínas não Estruturais Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Nonstructural Proteins); 77238-31-4 (Interferon-beta); EC 2.7.11.10 (I-kappa B Kinase); EC 3.4.22.- (3C-like proteinase, Coronavirus); EC 3.4.22.- (Cysteine Endopeptidases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170622
[Lr] Data última revisão:
170622
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161217
[St] Status:MEDLINE


  6 / 1706 MEDLINE  
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[PMID]:27718337
[Au] Autor:Saeng-Chuto K; Lorsirigool A; Temeeyasen G; Vui DT; Stott CJ; Madapong A; Tripipat T; Wegner M; Intrakamhaeng M; Chongcharoen W; Tantituvanont A; Kaewprommal P; Piriyapongsa J; Nilubol D
[Ad] Endereço:Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
[Ti] Título:Different Lineage of Porcine Deltacoronavirus in Thailand, Vietnam and Lao PDR in 2015.
[So] Source:Transbound Emerg Dis;64(1):3-10, 2017 Feb.
[Is] ISSN:1865-1682
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Porcine deltacoronavirus (PDCoV) was detected by RT-PCR in 12 of 97 (12.4%) intestinal samples collected during 2015 from piglets with diarrhoea in Thailand, Vietnam and Lao PDR. Spike, membrane and nucleocapsid genes were characterized, and phylogenetic analyses demonstrated that PDCoV isolates from Thai and Lao PDR form a novel cluster, separated from US and China isolates, but relatively were more closely related to China PDCoV than US isolates. Vietnam PDCoVs, however, were grouped together with US PDCoV. The analyses of amino acid changes suggested that they were from different lineage.
[Mh] Termos MeSH primário: Coronaviridae/genética
DNA Viral/genética
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Animais
Infecções por Coronaviridae/genética
Intestinos/virologia
Laos
Filogenia
Reação em Cadeia da Polimerase
Suínos
Tailândia
Vietnã
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170209
[Lr] Data última revisão:
170209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161009
[St] Status:MEDLINE
[do] DOI:10.1111/tbed.12585


  7 / 1706 MEDLINE  
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[PMID]:27754363
[Au] Autor:Todt D; Walter S; Brown RJ; Steinmann E
[Ad] Endereço:Institute of Experimental Virology, Twincore-Centre for Experimental and Clinical Infection Research, a Joint Venture between the Medical School Hannover (MHH) and the Helmholtz Centre for Infection Research, 30625 Hannover, Germany. Daniel.Todt@twincore.de.
[Ti] Título:Mutagenic Effects of Ribavirin on Hepatitis E Virus-Viral Extinction versus Selection of Fitness-Enhancing Mutations.
[So] Source:Viruses;8(10), 2016 Oct 13.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Hepatitis E virus (HEV), an important agent of viral hepatitis worldwide, can cause severe courses of infection in pregnant women and immunosuppressed patients. To date, HEV infections can only be treated with ribavirin (RBV). Major drawbacks of this therapy are that RBV is not approved for administration to pregnant women and that the virus can acquire mutations, which render the intra-host population less sensitive or even resistant to RBV. One of the proposed modes of action of RBV is a direct mutagenic effect on viral genomes, inducing mismatches and subsequent nucleotide substitutions. These transition events can drive the already error-prone viral replication beyond an error threshold, causing viral population extinction. In contrast, the expanded heterogeneous viral population can facilitate selection of mutant viruses with enhanced replication fitness. Emergence of these mutant viruses can lead to therapeutic failure. Consequently, the onset of RBV treatment in chronically HEV-infected individuals can result in two divergent outcomes: viral extinction versus selection of fitness-enhanced viruses. Following an overview of RNA viruses treated with RBV in clinics and a summary of the different antiviral modes of action of this drug, we focus on the mutagenic effect of RBV on HEV intrahost populations, and how HEV is able to overcome lethal mutagenesis.
[Mh] Termos MeSH primário: Antivirais/farmacologia
Vírus da Hepatite E/efeitos dos fármacos
Vírus da Hepatite E/genética
Mutagênicos/farmacologia
Mutação
Ribavirina/farmacologia
Seleção Genética
[Mh] Termos MeSH secundário: Antivirais/uso terapêutico
Coronaviridae
Farmacorresistência Viral
Hepatite E/tratamento farmacológico
Hepatite E/virologia
Seres Humanos
Mutagênicos/uso terapêutico
Ribavirina/uso terapêutico
Resultado do Tratamento
Virulência
Vírus
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Mutagens); 49717AWG6K (Ribavirin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161019
[St] Status:MEDLINE


  8 / 1706 MEDLINE  
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[PMID]:27599927
[Au] Autor:Moutelíková R; Dufková L; Kamler J; Drimaj J; Plhal R; Prodelalová J
[Ad] Endereço:Department of Virology, Veterinary Research Institute, Hudcova 70, 62100 Brno, Czech Republic. Electronic address: moutelikova@vri.cz.
[Ti] Título:Epidemiological survey of enteric viruses in wild boars in the Czech Republic: First evidence of close relationship between wild boar and human rotavirus A strains.
[So] Source:Vet Microbiol;193:28-35, 2016 Sep 25.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Population of wild boar is increasing in the whole Europe, the animals migrate close to human habitats which greatly increases the possibility of natural transmission between domestic animals or humans and wild boars. The aim of the study was to estimate in population of free-living wild boar in the Czech Republic the prevalence of enteric viral pathogens, namely rotavirus groups A and C (RVA and RVC), porcine reproductive and respiratory syndrome virus (PRRSV), and members of family Coronaviridae (transmissible gastroenteritis virus - TGEV, porcine epidemic diarrhea virus - PEDV, porcine respiratory coronavirus - PRCV, and porcine hemagglutination encephalomyelitis virus - PHEV) and Picornaviridae,(teschovirus A - PTV, sapelovirus A - PSV, and enterovirus G - EV-G). In our study, stool samples from 203 wild boars culled during hunting season 2014-2015 (from October to January) were examined by RT-PCR. RVA was detected in 2.5% of tested samples. Nucleotide analysis of VP7, VP4, and VP6 genes revealed that four RVA strains belong to G4P[25]I1, G4P[6]I5, G11P[13]I5, and G5P[13]I5 genotypes and phylogenetic analysis suggested close relation to porcine and human RVAs. The prevalence of RVC in wild boar population reached 12.8%, PTV was detected in 20.2%, PSV in 8.9%, and EV-G in 2.5% of samples. During our study no PRRSV or coronaviruses were detected. Our study provides the first evidence of RVC prevalence in wild boars and indicates that wild boars might contribute to the genetic variability of RVA and also serve as an important reservoir of other enteric viruses.
[Mh] Termos MeSH primário: Infecções por Coronaviridae/veterinária
Infecções por Picornaviridae/veterinária
Infecções por Rotavirus/veterinária
Rotavirus/isolamento & purificação
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Animais
Antígenos Virais/genética
Proteínas do Capsídeo/genética
Coronaviridae/genética
Coronaviridae/isolamento & purificação
Infecções por Coronaviridae/epidemiologia
Infecções por Coronaviridae/virologia
República Tcheca/epidemiologia
Reservatórios de Doenças
Fezes/virologia
Feminino
Genótipo
Seres Humanos
Masculino
Filogenia
Picornaviridae/genética
Picornaviridae/isolamento & purificação
Infecções por Picornaviridae/epidemiologia
Infecções por Picornaviridae/virologia
Rotavirus/genética
Infecções por Rotavirus/epidemiologia
Infecções por Rotavirus/virologia
Sus scrofa
Suínos
Doenças dos Suínos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Viral); 0 (Capsid Proteins); 0 (VP4 protein, Rotavirus); 0 (VP6 protein, Rotavirus); 0 (VP7 protein, Rotavirus)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160908
[St] Status:MEDLINE


  9 / 1706 MEDLINE  
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[PMID]:27578872
[Au] Autor:Vitosh-Sillman S; Loy JD; Brodersen B; Kelling C; Doster A; Topliff C; Nelson E; Bai J; Schirtzinger E; Poulsen E; Meadors B; Anderson J; Hause B; Anderson G; Hesse R
[Ad] Endereço:School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE (Vitosh-Sillman, Loy, Brodersen, Kelling, Doster, Topliff)Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, SD (Nelson)Kansas State Veterinary Diagnostic Labo
[Ti] Título:Experimental infection of conventional nursing pigs and their dams with Porcine deltacoronavirus.
[So] Source:J Vet Diagn Invest;28(5):486-97, 2016 Sep.
[Is] ISSN:1943-4936
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Porcine deltacoronavirus (PDCoV) is a newly identified virus that has been detected in swine herds of North America associated with enteric disease. The aim of this study was to demonstrate the pathogenicity, course of infection, virus kinetics, and aerosol transmission of PDCoV using 87 conventional piglets and their 9 dams, including aerosol and contact controls to emulate field conditions. Piglets 2-4 days of age and their dams were administered an oronasal PDCoV inoculum with a quantitative real-time reverse transcription (qRT)-PCR quantification cycle (Cq) value of 22 that was generated from a field sample having 100% nucleotide identity to USA/Illinois121/2014 determined by metagenomic sequencing and testing negative for other enteric disease agents using standard assays. Serial samples of blood, serum, oral fluids, nasal and fecal swabs, and tissues from sequential autopsy, conducted daily on days 1-8 and regular intervals thereafter, were collected throughout the 42-day study for qRT-PCR, histopathology, and immunohistochemistry. Diarrhea developed in all inoculated and contact control pigs, including dams, by 2 days post-inoculation (dpi) and in aerosol control pigs and dams by 3-4 dpi, with resolution occurring by 12 dpi. Mild to severe atrophic enteritis with PDCoV antigen staining was observed in the small intestine of affected piglets from 2 to 8 dpi. Mesenteric lymph node and small intestine were the primary sites of antigen detection by immunohistochemistry, and virus RNA was detected in these tissues to the end of the study. Virus RNA was detectable in piglet fecal swabs to 21 dpi, and dams to 14-35 dpi.
[Mh] Termos MeSH primário: Coronaviridae/patogenicidade
Infecções por Coronavirus/veterinária
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Coronaviridae/genética
Infecções por Coronavirus/virologia
Diarreia/veterinária
Feminino
Imuno-Histoquímica/veterinária
RNA Viral/genética
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Análise de Sobrevida
Suínos
Doenças dos Suínos/mortalidade
Doenças dos Suínos/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160901
[St] Status:MEDLINE
[do] DOI:10.1177/1040638716654200


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[PMID]:27496131
[Au] Autor:Zhai SL; Wei WK; Li XP; Wen XH; Zhou X; Zhang H; Lv DH; Li F; Wang D
[Ad] Endereço:Animal Disease Diagnostic Center, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of Animal Disease Prevention, Guangdong Open Laboratory of Veterinary Public Health, Guangzhou, 510640, China.
[Ti] Título:Occurrence and sequence analysis of porcine deltacoronaviruses in southern China.
[So] Source:Virol J;13:136, 2016 Aug 05.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Following the initial isolation of porcine deltacoronavirus (PDCoV) from pigs with diarrheal disease in the United States in 2014, the virus has been detected on swine farms in some provinces of China. To date, little is known about the molecular epidemiology of PDCoV in southern China where major swine production is operated. RESULTS: To investigate the prevalence of PDCoV in this region and compare its activity to other enteric disease of swine caused by porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis coronavirus (TGEV), and porcine rotavirus group C (Rota C), 390 fecal samples were collected from swine of various ages from 15 swine farms with reported diarrhea. Fecal samples were tested by reverse transcription-PCR (RT-PCR) that targeted PDCoV, PEDV, TGEV, and Rota C, respectively. PDCoV was detected exclusively from nursing piglets with an overall prevalence of approximate 1.28 % (5/390), not in suckling and fattening piglets. Interestingly, all of PDCoV-positive samples were from 2015 rather than 2012-2014. Despite a low detection rate, PDCoV emerged in each province/region of southern China. In addition, compared to TGEV (1.54 %, 5/390) or Rota C (1.28 %, 6/390), there were highly detection rates of PEDV (22.6 %, 88/390) in those samples. Notably, all five PDCoV-positive piglets were co-infected by PEDV. Furthermore, phylogenetic analysis of spike (S) and nucleocapsid (N) gene sequences of PDCoVs revealed that currently circulating PDCoVs in southern China were more closely related to other Chinese strains of PDCoVs than to those reported in United States, South Korea and Thailand. CONCLUSIONS: This study demonstrated that PDCoV was present in southern China despite the low prevalence, and supported an evolutionary theory of geographical clustering of PDCoVs.
[Mh] Termos MeSH primário: Infecções por Coronaviridae/veterinária
Coronaviridae/isolamento & purificação
Fezes/virologia
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Animais
China/epidemiologia
Coronaviridae/classificação
Coronaviridae/genética
Infecções por Coronaviridae/epidemiologia
Infecções por Coronaviridae/virologia
Filogenia
Análise de Sequência de DNA
Suínos
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160807
[St] Status:MEDLINE
[do] DOI:10.1186/s12985-016-0591-6



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