Base de dados : MEDLINE
Pesquisa : B04.820.504.540.150.075.500.500 [Categoria DeCS]
Referências encontradas : 718 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 72 ir para página                         

  1 / 718 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28448848
[Au] Autor:Ding Z; An K; Xie L; Wu W; Zhang R; Wang D; Fang Y; Chen H; Xiao S; Fang L
[Ad] Endereço:State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, the Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
[Ti] Título:Transmissible gastroenteritis virus infection induces NF-κB activation through RLR-mediated signaling.
[So] Source:Virology;507:170-178, 2017 07.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Transmissible gastroenteritis virus (TGEV) is a porcine enteric coronavirus which causes lethal severe watery diarrhea in piglets. The pathogenesis of TGEV is strongly associated with inflammation. In this study, we found that TGEV infection activates transcription factors NF-κB, IRF3 and AP-1 in a time- and dose-dependent manner in porcine kidney cells. Treatment with the NF-κB-specific inhibitor BAY11-7082 significantly decreased TGEV-induced proinflammatory cytokine production, but did not affect virus replication. Phosphorylation of NF-κB subunit p65 and proinflammatory cytokine production were greatly decreased after knockdown of retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) or its adaptors MAVS and STING, while only slight reduction was observed in cells following silencing of Toll-like receptor adaptors, MyD88 and TRIF. Furthermore, TGEV infection significantly upregulated mRNA expression of RIG-I and MDA5. Taken together, our results indicate that the RLR signaling pathway is involved in TGEV-induced inflammatory responses.
[Mh] Termos MeSH primário: Gastroenterite Suína Transmissível/imunologia
Gastroenterite Suína Transmissível/virologia
NF-kappa B/imunologia
Vírus da Gastroenterite Transmissível/fisiologia
[Mh] Termos MeSH secundário: Animais
Citocinas/genética
Citocinas/imunologia
Proteína DEAD-box 58/genética
Proteína DEAD-box 58/imunologia
Gastroenterite Suína Transmissível/genética
Helicase IFIH1 Induzida por Interferon/genética
Helicase IFIH1 Induzida por Interferon/imunologia
NF-kappa B/genética
Fosforilação
Transdução de Sinais
Suínos
Fator de Transcrição AP-1/genética
Fator de Transcrição AP-1/imunologia
Vírus da Gastroenterite Transmissível/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokines); 0 (NF-kappa B); 0 (Transcription Factor AP-1); EC 3.6.4.13 (DEAD Box Protein 58); EC 3.6.4.13 (Interferon-Induced Helicase, IFIH1)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171220
[Lr] Data última revisão:
171220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE


  2 / 718 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28542235
[Au] Autor:Trudeau MP; Verma H; Sampedro F; Urriola PE; Shurson GC; Goyal SM
[Ad] Endereço:Department of Animal Science, University of Minnesota, St. Paul, Minnesota, United States of America.
[Ti] Título:Environmental persistence of porcine coronaviruses in feed and feed ingredients.
[So] Source:PLoS One;12(5):e0178094, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Porcine Epidemic Diarrhea Virus (PEDV), Porcine Delta Corona Virus (PDCoV), and Transmissible Gastroenteritis Virus (TGEV) are major threats to swine health and contaminated feed plays a role in virus transmission. The objective of our study was to characterize inactivation of PEDV, PDCoV, and TGEV in various feed ingredient matrices. Samples of complete feed, spray dried porcine plasma, meat meal, meat and bone meal, blood meal, corn, soybean meal, and corn dried distillers grains with solubles were weighed (5 g/sample) into scintillation vials and inoculated with 1 mL of PEDV, PDCoV, or TGEV. Samples were incubated at room temperature for up to 56 days. Aliquots were removed at various time points followed by preparing serial 10-fold dilutions and inoculating in cell cultures to determine the amount of surviving virus. Inactivation kinetics were determined using the Weibull model, which estimates a delta value indicating the time necessary to reduce virus concentration by 1 log. Delta values of various ingredients were compared and analyzed as to their nutrient composition. Soybean meal had the greatest delta value (7.50 days) for PEDV (P < 0.06) as compared with all other ingredients. High delta values (P < 0.001) were observed in soybean meal for PDCoV (42.04 days) and TGEV (42.00 days). There was a moderate correlation between moisture content and the delta value for PDCoV (r = 0.49, P = 0.01) and TGEV (r = 0.41, P = 0.02). There was also a moderate negative correlation between TGEV survival and ether extract content (r = -0.51, P = 0.01). In conclusion, these results indicate that the first log reduction of PDCoV and TGEV takes the greatest amount of time in soybean meal. In addition to this, moisture and ether content appear to be an important determinant of virus survival in feed ingredients.
[Mh] Termos MeSH primário: Ração Animal/virologia
Coronavirus/isolamento & purificação
Contaminação de Alimentos
Vírus da Diarreia Epidêmica Suína/isolamento & purificação
Vírus da Gastroenterite Transmissível/isolamento & purificação
[Mh] Termos MeSH secundário: Ração Animal/análise
Animais
Infecções por Coronavirus/transmissão
Infecções por Coronavirus/veterinária
Infecções por Coronavirus/virologia
Gastroenterite Suína Transmissível/transmissão
Feijão de Soja/química
Sus scrofa
Suínos
Doenças dos Suínos/transmissão
Doenças dos Suínos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178094


  3 / 718 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28438630
[Au] Autor:Yang CW; Chang HY; Hsu HY; Lee YZ; Chang HS; Chen IS; Lee SJ
[Ad] Endereço:Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli 35053, Taiwan, ROC.
[Ti] Título:Identification of anti-viral activity of the cardenolides, Na /K -ATPase inhibitors, against porcine transmissible gastroenteritis virus.
[So] Source:Toxicol Appl Pharmacol;332:129-137, 2017 Oct 01.
[Is] ISSN:1096-0333
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A series of naturally occurring cardenolides that exhibit potent anti-transmissible gastroenteritis virus (TGEV) activity in swine testicular (ST) cells has been identified. In an immunofluorescence assay, these cardenolides were found to diminish the expressions of TGEV nucleocapsid and spike protein, which was used as an indication for viral replication; block TGEV infection induced apoptosis and cytopathic effects; and impart the same trend of inhibitory activity against Na /K -ATPase as for anti-TGEV activity. The viral titer inhibition was found to take place in a dose-dependent manner. Knocking down expression of Na /K -ATPase, the cellular receptor of cardenolides, in ST cells was found to significantly impair the susceptibility of ST cells to TGEV infectivity. Thus, we have identified Na /K -ATPase as an anti-viral drug target and its antagonists, cardenolides, a novel class of anti- TGEV agents.
[Mh] Termos MeSH primário: Antivirais/farmacologia
Cardenolídeos/farmacologia
ATPase Trocadora de Sódio-Potássio/antagonistas & inibidores
Vírus da Gastroenterite Transmissível/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Anticorpos Monoclonais/farmacologia
Apoptose/efeitos dos fármacos
Relação Dose-Resposta a Droga
Células Epiteliais/efeitos dos fármacos
Células Epiteliais/metabolismo
Inativação Gênica
Proteínas do Nucleocapsídeo/genética
Proteínas do Nucleocapsídeo/metabolismo
RNA Viral/isolamento & purificação
ATPase Trocadora de Sódio-Potássio/metabolismo
Suínos
Vírus da Gastroenterite Transmissível/fisiologia
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Antiviral Agents); 0 (Cardenolides); 0 (Nucleocapsid Proteins); 0 (RNA, Viral); EC 3.6.3.9 (Sodium-Potassium-Exchanging ATPase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170426
[St] Status:MEDLINE


  4 / 718 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28299912
[Au] Autor:Zhang X; Zhu Y; Zhu X; Chen J; Shi H; Shi D; Dong H; Feng L
[Ad] Endereço:State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China.
[Ti] Título:ORF3a deletion in field strains of porcine-transmissible gastroenteritis virus in China: A hint of association with porcine respiratory coronavirus.
[So] Source:Transbound Emerg Dis;64(3):698-702, 2017 Jun.
[Is] ISSN:1865-1682
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Porcine-transmissible gastroenteritis virus (TGEV) is a pathogenic coronavirus responsible for high diarrhoea-associated morbidity and mortality in suckling piglets. We analysed the TGEV ORF3 gene using nested polymerase chain reaction and identified an ORF3a deletion in three field strains of TGEV collected from piglets in China in 2015. Eight TGEV ORF3 sequences were obtained in this study. Phylogenetic tree analysis of ORF3 showed that the eight TGEV ORF3 genes all belonged to the Miller cluster. CH-LNCT and CH-MZL were closely correlated with Miller M6, while CH-SH was correlated with Miller M60. These results thus indicate that the existence of Miller, as well as the Purdue cluster, in Chinese field strains of TGEV. Furthermore, we found the first evidence for a large deletion in ORF3 resulting in the loss of ORF3a, previously reported in porcine respiratory coronavirus, in three field strains (CH-LNCT, CH-MZL, and CH-SH) of TGEV. The results of the present study thus provide important information regarding the underlying evolution mechanisms of coronaviruses.
[Mh] Termos MeSH primário: Infecções por Coronavirus/veterinária
Gastroenterite Suína Transmissível/virologia
Coronavirus Respiratório Porcino/isolamento & purificação
Vírus da Gastroenterite Transmissível/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
China/epidemiologia
Infecções por Coronavirus/complicações
Infecções por Coronavirus/virologia
Gastroenterite Suína Transmissível/complicações
Gastroenterite Suína Transmissível/epidemiologia
Filogenia
Reação em Cadeia da Polimerase
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE
[do] DOI:10.1111/tbed.12634


  5 / 718 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28203548
[Au] Autor:Xing Y; Liqi Z; Jian L; Qinghua Y; Qian Y
[Ad] Endereço:Key Laboratory of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University Nanjing, China.
[Ti] Título:Doxycycline Induces Mitophagy and Suppresses Production of Interferon-ß in IPEC-J2 Cells.
[So] Source:Front Cell Infect Microbiol;7:21, 2017.
[Is] ISSN:2235-2988
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Previous reports have demonstrated that the second-generation tetracycline derivative doxycycline (DOX) interrupts mitochondrial proteostasis and physiology, inhibits proliferation of many cell types, and induces apoptosis. However, the effects of DOX, which is widely used in porcine husbandry by feed, on the porcine intestinal epithelium are unclear. In this study, we demonstrated that DOX damaged mitochondrial morphology and induced the co-localization of mitochondria with autophagosomes, suggesting that DOX induces mitophagy in IPEC-J2 cells. We also found evidence that DOX increased intracellular levels of reactive oxygen species (ROS) or mitochondrial-specific ROS in a dose dependent manner. Moreover, 50 µg/ml DOX significantly decreased production of interferon-ß and facilitated replication of transmissible gastroenteritis coronavirus in IPEC-J2 cells. These results demonstrated that DOX induced mitophagy and ROS production, which damaged the intestinal epithelium. As DOX is used extensively in pig husbandry, uncontrolled application poses a significant threat of viral infection, so stricter policies on its usage should be required.
[Mh] Termos MeSH primário: Doxiciclina/farmacologia
Interferon beta/biossíntese
Degradação Mitocondrial/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Autofagia/efeitos dos fármacos
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Células Epiteliais/efeitos dos fármacos
Células Epiteliais/metabolismo
Células Epiteliais/virologia
Imunidade Inata/efeitos dos fármacos
Mucosa Intestinal/efeitos dos fármacos
Mucosa Intestinal/metabolismo
Mucosa Intestinal/virologia
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/metabolismo
Espécies Reativas de Oxigênio/metabolismo
Suínos
Vírus da Gastroenterite Transmissível/fisiologia
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Reactive Oxygen Species); 77238-31-4 (Interferon-beta); N12000U13O (Doxycycline)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170217
[St] Status:MEDLINE
[do] DOI:10.3389/fcimb.2017.00021


  6 / 718 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28202790
[Au] Autor:Gimenez-Lirola LG; Zhang J; Carrillo-Avila JA; Chen Q; Magtoto R; Poonsuk K; Baum DH; Piñeyro P; Zimmerman J
[Ad] Endereço:College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA luisggl@iastate.edu.
[Ti] Título:Reactivity of Porcine Epidemic Diarrhea Virus Structural Proteins to Antibodies against Porcine Enteric Coronaviruses: Diagnostic Implications.
[So] Source:J Clin Microbiol;55(5):1426-1436, 2017 May.
[Is] ISSN:1098-660X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The development of porcine epidemic diarrhea virus (PEDV) antibody-based assays is important for detecting infected animals, confirming previous virus exposure, and monitoring sow herd immunity. However, the potential cross-reactivity among porcine coronaviruses is a major concern for the development of pathogen-specific assays. In this study, we used serum samples ( = 792) from pigs of precisely known infection status and a multiplex fluorescent microbead-based immunoassay and/or enzyme-linked immunoassay platform to characterize the antibody response to PEDV whole-virus (WV) particles and recombinant polypeptides derived from the four PEDV structural proteins, i.e., spike (S), nucleocapsid (N), membrane (M), and envelope (E). Antibody assay cutoff values were selected to provide 100% diagnostic specificity for each target. The earliest IgG antibody response, mainly directed against S1 polypeptides, was observed at days 7 to 10 postinfection. With the exception of nonreactive protein E, we observed similar antibody ontogenies and patterns of seroconversion for S1, N, M, and WV antigens. Recombinant S1 provided the best diagnostic sensitivity, regardless of the PEDV strain, with no cross-reactivity detected against transmissible gastroenteritis virus (TGEV), porcine respiratory coronavirus (PRCV), or porcine deltacoronavirus (PDCoV) pig antisera. The WV particles showed some cross-reactivity to TGEV Miller and TGEV Purdue antisera, while N protein presented some cross-reactivity to TGEV Miller. The M protein was highly cross-reactive to TGEV and PRCV antisera. Differences in the antibody responses to specific PEDV structural proteins have important implications in the development and performance of antibody assays for the diagnosis of PEDV enteric disease.
[Mh] Termos MeSH primário: Antígenos Virais/imunologia
Infecções por Coronavirus
Vírus da Diarreia Epidêmica Suína/imunologia
Doenças dos Suínos/diagnóstico
Suínos/virologia
Vírus da Gastroenterite Transmissível/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/sangue
Anticorpos Antivirais/imunologia
Infecções por Coronavirus/diagnóstico
Infecções por Coronavirus/veterinária
Infecções por Coronavirus/virologia
Reações Cruzadas/imunologia
Diagnóstico Diferencial
Imunoglobulina G/sangue
Imunoglobulina G/imunologia
Proteínas do Nucleocapsídeo/imunologia
Doenças dos Suínos/virologia
Proteínas da Matriz Viral/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Antigens, Viral); 0 (Immunoglobulin G); 0 (Nucleocapsid Proteins); 0 (Viral Matrix Proteins); 0 (nucleocapsid protein, Coronavirus); 108502-71-2 (M protein, Coronavirus)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170217
[St] Status:MEDLINE
[do] DOI:10.1128/JCM.02507-16


  7 / 718 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28110779
[Au] Autor:Zhu L; Yang X; Mou C; Yang Q
[Ad] Endereço:Veterinary College, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China.
[Ti] Título:Transmissible gastroenteritis virus does not suppress IFN-ß induction but is sensitive to IFN in IPEC-J2 cells.
[So] Source:Vet Microbiol;199:128-134, 2017 Feb.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Coronaviruses tend to efficiently evade innate immune sensing. Alpha-coronaviruses interfere with the type I interferon (IFN) response in various ways, ensuring the limited activation of IFN responses. Transmissible gastroenteritis virus (TGEV), an Alphacoronavirus genera virus, is an important pathogen that mainly infects piglet, but little is known about the activation of the host immune response. We show that TGEV induces a delayed activation of the IFN response in intestinal epithelial cells. Briefly, IFN-ß expression induced by TGEV infection is delayed with respect to that induced by poly(I:C) transfection. In addition, some of the IFN-stimulated genes (ISGs) were up-regulated in the early infection stage without obvious expression of IFN-ß. Moreover, we show that activation of IFN responses induced by poly(I:C) could inhibit viral replication in the early infection stage, but failed in the late infection stage in IPEC-J2 cells. Finally, the activation of IFN responses induced by TGEV infection cannot inhibit viral replication. Taken together, this study provides a preliminary analysis of an interaction between TGEV and IFN-ß responses of intestinal epithelial cells.
[Mh] Termos MeSH primário: Células Epiteliais/imunologia
Células Epiteliais/virologia
Gastroenterite Suína Transmissível/imunologia
Regulação da Expressão Gênica/imunologia
Interações Hospedeiro-Patógeno/imunologia
Vírus da Gastroenterite Transmissível/imunologia
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Gastroenterite Suína Transmissível/virologia
Técnicas de Inativação de Genes
Interações Hospedeiro-Patógeno/efeitos dos fármacos
Fatores Imunológicos/farmacologia
Interferon Tipo I/imunologia
Interferon beta/genética
Poli I-C/farmacologia
Receptores de Interferon/genética
Suínos
Replicação Viral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunologic Factors); 0 (Interferon Type I); 0 (Receptors, Interferon); 77238-31-4 (Interferon-beta); O84C90HH2L (Poly I-C)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE


  8 / 718 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27848068
[Au] Autor:Wang L; Dai X; Song H; Yuan P; Yang Z; Dong W; Song Z
[Ad] Endereço:Department of Veterinary Medicine, Southwest University, Rongchang Campus, Chongqing, 402460, People's Republic of China.
[Ti] Título:Inhibition of porcine transmissible gastroenteritis virus infection in porcine kidney cells using short hairpin RNAs targeting the membrane gene.
[So] Source:Virus Genes;53(2):226-232, 2017 Apr.
[Is] ISSN:1572-994X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The membrane (M) protein is the most abundant component of the porcine transmissible gastroenteritis virus (TGEV) particle. To exploit the possibility of using RNA interference (RNAi) as a strategy against TGEV infection, three plasmids (pRNAT-1, pRNAT-2, and pRNAT-3) expressing short hairpin RNAs were designed to target three different coding regions of the M gene of TGEV. The plasmids were constructed and transiently transfected into a porcine kidney cells, PK-15, to determine whether these constructs inhibited TGEV production. The analysis of cytopathic effects demonstrated that pRNAT-2 and pRNAT-3 could protect PK-15 cells against pathological changes specifically and efficiently. Additionally, indirect immunofluorescence and 50% tissue culture infectious dose (TCID ) assays showed that pRNAT-2 and pRNAT-3 inhibited the multiplication of the virus at the protein level effectively. Quantitative real-time PCR further confirmed that the amounts of viral RNAs in cell cultures pre-transfected with the three plasmids were reduced by 13, 68, and 70%, respectively. This is the first report showing that RNAi targeting of the M gene. Our results could promote studies of the specific function of viral genes associated with TGEV infection and might provide a theoretical basis for potential therapeutic applications.
[Mh] Termos MeSH primário: Gastroenterite Suína Transmissível/genética
Proteínas de Membrana/genética
RNA Interferente Pequeno/genética
Vírus da Gastroenterite Transmissível/genética
[Mh] Termos MeSH secundário: Animais
Gastroenterite Suína Transmissível/terapia
Gastroenterite Suína Transmissível/virologia
Rim/patologia
Rim/virologia
Proteínas de Membrana/antagonistas & inibidores
Interferência de RNA
Suínos/genética
Suínos/virologia
Vírus da Gastroenterite Transmissível/patogenicidade
Replicação Viral/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Membrane Proteins); 0 (RNA, Small Interfering)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161117
[St] Status:MEDLINE
[do] DOI:10.1007/s11262-016-1409-8


  9 / 718 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27815750
[Au] Autor:Zhu Y; Liang L; Luo Y; Wang G; Wang C; Cui Y; Ai X; Cui S
[Ad] Endereço:College of Animal Science and Technology, HLJ August First Land Reclamation University, Daqing, 163319, China.
[Ti] Título:A sensitive duplex nanoparticle-assisted PCR assay for identifying porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus from clinical specimens.
[So] Source:Virus Genes;53(1):71-76, 2017 Feb.
[Is] ISSN:1572-994X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, a novel duplex nanoparticle-assisted polymerase chain reaction (nanoPCR) assay was developed to detect porcine epidemic diarrhea virus (PEDV) and porcine transmissible gastroenteritis virus (TGEV). Two pairs of primers were designed based on the conserved region within the N gene of PEDV and TGEV. In a screening of 114 clinical samples from four provinces in China for PEDV and TGEV, 48.2 and 3.5 % of the samples, respectively, tested positive. Under optimized conditions, the duplex nanoPCR assay had a detection limit of 7.6 × 10 and 8.5 × 10 copies µL for PEDV and TGEV, respectively. The sensitivity of the duplex nanoPCR assay was ten times higher than that of a conventional PCR assay. Moreover, no fragments were amplified when the duplex nanoPCR assay was used to test samples containing other porcine viruses. Our results indicate that the duplex nanoPCR assay described here is useful for the rapid detection of PEDV and TGEV and can be applied in clinical diagnosis.
[Mh] Termos MeSH primário: Nanopartículas
Reação em Cadeia da Polimerase
Vírus da Diarreia Epidêmica Suína/genética
Doenças dos Suínos/diagnóstico
Doenças dos Suínos/virologia
Vírus da Gastroenterite Transmissível/genética
[Mh] Termos MeSH secundário: Animais
Reação em Cadeia da Polimerase/métodos
Vírus da Diarreia Epidêmica Suína/classificação
Vírus da Diarreia Epidêmica Suína/isolamento & purificação
Sensibilidade e Especificidade
Suínos
Vírus da Gastroenterite Transmissível/classificação
Vírus da Gastroenterite Transmissível/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161106
[St] Status:MEDLINE
[do] DOI:10.1007/s11262-016-1405-z


  10 / 718 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
[PMID]:27973413
[Au] Autor:Dong H; Zhang X; Shi H; Chen J; Shi D; Zhu Y; Feng L
[Ad] Endereço:State Key Laboratory of Veterinary Biotechnology, Division of Swine Infectious Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. donghui_12215084@126.com.
[Ti] Título:Characterization of an Immunodominant Epitope in the Endodomain of the Coronavirus Membrane Protein.
[So] Source:Viruses;8(12), 2016 Dec 10.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The coronavirus membrane (M) protein acts as a dominant immunogen and is a major player in virus assembly. In this study, we prepared two monoclonal antibodies (mAbs; 1C3 and 4C7) directed against the transmissible gastroenteritis virus (TGEV) M protein. The 1C3 and 4C7 mAbs both reacted with the native TGEV M protein in western blotting and immunofluorescence (IFA) assays. Two linear epitopes, 243YSTEART249 (1C3) and 243YSTEARTDNLSEQEKLLHMV262 (4C7), were identified in the endodomain of the TGEV M protein. The 1C3 mAb can be used for the detection of the TGEV M protein in different assays. An IFA method for the detection of TGEV M protein was optimized using mAb 1C3. Furthermore, the ability of the epitope identified in this study to stimulate antibody production was also evaluated. An immunodominant epitope in the TGEV membrane protein endodomain was identified. The results of this study have implications for further research on TGEV replication.
[Mh] Termos MeSH primário: Epitopos Imunodominantes/imunologia
Vírus da Gastroenterite Transmissível/imunologia
Proteínas da Matriz Viral/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Monoclonais/imunologia
Anticorpos Monoclonais/isolamento & purificação
Anticorpos Antivirais/imunologia
Anticorpos Antivirais/isolamento & purificação
Western Blotting
Linhagem Celular
Mapeamento de Epitopos
Imunofluorescência
Camundongos Endogâmicos BALB C
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Antibodies, Viral); 0 (Immunodominant Epitopes); 0 (Viral Matrix Proteins); 108502-71-2 (M protein, Coronavirus)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161216
[St] Status:MEDLINE



página 1 de 72 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde