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  1 / 23 MEDLINE  
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[PMID]:25463600
[Au] Autor:Wang W; Lin XD; Guo WP; Zhou RH; Wang MR; Wang CQ; Ge S; Mei SH; Li MH; Shi M; Holmes EC; Zhang YZ
[Ad] Endereço:State Key Laboratory for Infectious Disease Prevention and Control, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China; Collaborative Innovation Center for Diagnosis and Treatment of
[Ti] Título:Discovery, diversity and evolution of novel coronaviruses sampled from rodents in China.
[So] Source:Virology;474:19-27, 2015 Jan 01.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Although rodents are important reservoirs for RNA viruses, to date only one species of rodent coronavirus (CoV) has been identified. Herein, we describe a new CoV, denoted Lucheng Rn rat coronavirus (LRNV), and novel variants of two Betacoronavirus species termed Longquan Aa mouse coronavirus (LAMV) and Longquan Rl rat coronavirus (LRLV), that were identified in a survey of 1465 rodents sampled in China during 2011-2013. Phylogenetic analysis revealed that LAMV and LRLV fell into lineage A of the genus Betacoronavirus, which included CoVs discovered in humans and domestic and wild animals. In contrast, LRNV harbored by Rattus norvegicus formed a distinct lineage within the genus Alphacoronavirus in the 3CL(pro), RdRp, and Hel gene trees, but formed a more divergent lineage in the N and S gene trees, indicative of a recombinant origin. Additional recombination events were identified in LRLV. Together, these data suggest that rodents may carry additional unrecognized CoVs.
[Mh] Termos MeSH primário: Coronavirus/genética
Coronavirus/isolamento & purificação
Roedores/virologia
[Mh] Termos MeSH secundário: Animais
China
Coronavirus/classificação
Coronavirus do Rato/classificação
Coronavirus do Rato/genética
Coronavirus do Rato/isolamento & purificação
Reservatórios de Doenças/virologia
Evolução Molecular
Variação Genética
Genoma Viral
Seres Humanos
Camundongos
Dados de Sequência Molecular
Filogenia
Ratos
Glicoproteína da Espícula de Coronavírus/química
Glicoproteína da Espícula de Coronavírus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Spike Glycoprotein, Coronavirus)
[Em] Mês de entrada:1504
[Cu] Atualização por classe:141208
[Lr] Data última revisão:
141208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE


  2 / 23 MEDLINE  
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[PMID]:24323639
[Au] Autor:Haick AK; Rzepka JP; Brandon E; Balemba OB; Miura TA
[Ad] Endereço:Department of Biological Sciences, University of Idaho, 875 Perimeter Dr., MS 3051, Moscow, ID 83844-3051, USA.
[Ti] Título:Neutrophils are needed for an effective immune response against pulmonary rat coronavirus infection, but also contribute to pathology.
[So] Source:J Gen Virol;95(Pt 3):578-90, 2014 Mar.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Polymorphonuclear neutrophils (PMN) infiltrate the respiratory tract early after viral infection and can contribute to both host defence and pathology. Coronaviruses are important causes of respiratory tract infections, ranging from mild to severe depending on the viral strain. This study evaluated the role of PMN during a non-fatal pulmonary coronavirus infection in the natural host. Rat coronavirus (RCoV) causes respiratory disease in adult rats, characterized by an early PMN response, viral replication and inflammatory lesions in the lungs, mild weight loss and effective resolution of infection. To determine their role during RCoV infection, PMN were depleted and the effects on disease progression, viral replication, inflammatory response and lung pathology were analysed. Compared with RCoV infection in control animals, PMN-depleted rats had worsened disease with weight loss, clinical signs, mortality and prolonged pulmonary viral replication. PMN-depleted animals had fewer macrophages and lymphocytes in the respiratory tract, corresponding to lower chemokine levels. Combined with in vitro experiments showing that PMN express cytokines and chemokines in response to RCoV-infected alveolar epithelial cells, these findings support a role for PMN in eliciting an inflammatory response to RCoV infection. Despite their critical role in the protection from severe disease, the presence of PMN was correlated with haemorrhagic lesions, epithelial barrier permeability and cellular inflammation in the lungs. This study demonstrated that while PMN are required for an effective antiviral response, they also contribute to lung pathology during RCoV infection.
[Mh] Termos MeSH primário: Infecções por Coronavirus/veterinária
Coronavirus do Rato/imunologia
Neutrófilos/imunologia
Alvéolos Pulmonares/imunologia
Doenças dos Roedores/imunologia
[Mh] Termos MeSH secundário: Animais
Infecções por Coronavirus/imunologia
Infecções por Coronavirus/patologia
Infecções por Coronavirus/virologia
Coronavirus do Rato/fisiologia
Citocinas/imunologia
Masculino
Alvéolos Pulmonares/patologia
Alvéolos Pulmonares/virologia
Ratos
Ratos Endogâmicos F344
Doenças dos Roedores/patologia
Doenças dos Roedores/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Cytokines)
[Em] Mês de entrada:1405
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131211
[St] Status:MEDLINE
[do] DOI:10.1099/vir.0.061986-0


  3 / 23 MEDLINE  
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[PMID]:19741068
[Au] Autor:Funk CJ; Manzer R; Miura TA; Groshong SD; Ito Y; Travanty EA; Leete J; Holmes KV; Mason RJ
[Ad] Endereço:National Jewish Health, 1400 Jackson Street, Denver, CO 80206, USA.
[Ti] Título:Rat respiratory coronavirus infection: replication in airway and alveolar epithelial cells and the innate immune response.
[So] Source:J Gen Virol;90(Pt 12):2956-64, 2009 Dec.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The rat coronavirus sialodacryoadenitis virus (SDAV) causes respiratory infection and provides a system for investigating respiratory coronaviruses in a natural host. A viral suspension in the form of a microspray aerosol was delivered by intratracheal instillation into the distal lung of 6-8-week-old Fischer 344 rats. SDAV inoculation produced a 7 % body weight loss over a 5 day period that was followed by recovery over the next 7 days. SDAV caused focal lesions in the lung, which were most severe on day 4 post-inoculation (p.i.). Immunofluorescent staining showed that four cell types supported SDAV virus replication in the lower respiratory tract, namely Clara cells, ciliated cells in the bronchial airway and alveolar type I and type II cells in the lung parenchyma. In bronchial alveolar lavage fluid (BALF) a neutrophil influx increased the population of neutrophils to 45 % compared with 6 % of the cells in control samples on day 2 after mock inoculation. Virus infection induced an increase in surfactant protein SP-D levels in BALF of infected rats on days 4 and 8 p.i. that subsided by day 12. The concentrations of chemokines MCP-1, LIX and CINC-1 in BALF increased on day 4 p.i., but returned to control levels by day 8. Intratracheal instillation of rats with SDAV coronavirus caused an acute, self-limited infection that is a useful model for studying the early events of the innate immune response to respiratory coronavirus infections in lungs of the natural virus host.
[Mh] Termos MeSH primário: Infecções por Coronavirus
Coronavirus do Rato/patogenicidade
Células Epiteliais/virologia
Pulmão/virologia
Alvéolos Pulmonares/virologia
Replicação Viral
[Mh] Termos MeSH secundário: Animais
Infecções por Coronavirus/imunologia
Infecções por Coronavirus/fisiopatologia
Infecções por Coronavirus/virologia
Coronavirus do Rato/fisiologia
Citocinas/metabolismo
Imunidade Inata
Pulmão/citologia
Masculino
Alvéolos Pulmonares/citologia
Surfactantes Pulmonares/metabolismo
Ratos
Ratos Endogâmicos F344
Perda de Peso
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Cytokines); 0 (Pulmonary Surfactants)
[Em] Mês de entrada:0912
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090911
[St] Status:MEDLINE
[do] DOI:10.1099/vir.0.014282-0


  4 / 23 MEDLINE  
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[PMID]:17804032
[Au] Autor:Miura TA; Wang J; Holmes KV; Mason RJ
[Ad] Endereço:Department of Microbiology, University of Colorado Health Sciences Center, MS 8333, PO Box 6511, Aurora, CO 80045, USA. tanya.miura@uchsc.edu
[Ti] Título:Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines.
[So] Source:Virology;369(2):288-98, 2007 Dec 20.
[Is] ISSN:0042-6822
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We analyzed the ability of two rat coronavirus (RCoV) strains, sialodacryoadenitis virus (SDAV) and Parker's RCoV (RCoV-P), to infect rat alveolar type I cells and induce chemokine expression. Primary rat alveolar type II cells were transdifferentiated into the type I cell phenotype. Type I cells were productively infected with SDAV and RCoV-P, and both live virus and UV-inactivated virus induced mRNA and protein expression of three CXC chemokines: CINC-2, CINC-3, and LIX, which are neutrophil chemoattractants. Dual immunolabeling of type I cells for viral antigen and CXC chemokines showed that chemokines were expressed primarily by uninfected cells. Virus-induced chemokine expression was reduced by the IL-1 receptor antagonist, suggesting that IL-1 produced by infected cells induces uninfected cells to express chemokines. Primary cultures of alveolar epithelial cells are an important model for the early events in viral infection that lead to pulmonary inflammation.
[Mh] Termos MeSH primário: Quimiocinas CXC/biossíntese
Quimiocinas CXC/genética
Coronavirus do Rato/patogenicidade
Alvéolos Pulmonares/imunologia
Alvéolos Pulmonares/virologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Coronavirus do Rato/imunologia
Células Epiteliais/classificação
Células Epiteliais/imunologia
Células Epiteliais/virologia
Expressão Gênica
Mediadores da Inflamação/metabolismo
Alvéolos Pulmonares/citologia
RNA Mensageiro/biossíntese
RNA Mensageiro/genética
Ratos
Receptores de Interleucina-1/metabolismo
Receptores do Fator de Necrose Tumoral/metabolismo
Transdução de Sinais
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Chemokines, CXC); 0 (Gm1960 protein, rat); 0 (Inflammation Mediators); 0 (RNA, Messenger); 0 (Receptors, Interleukin-1); 0 (Receptors, Tumor Necrosis Factor)
[Em] Mês de entrada:0801
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070907
[St] Status:MEDLINE


  5 / 23 MEDLINE  
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[PMID]:17037558
[Au] Autor:Miura TA; Wang J; Mason RJ; Holmes KV
[Ad] Endereço:University of Colorado Health Sciences Center, Aurora 80010, USA.
[Ti] Título:Rat coronavirus infection of primary rat alveolar epithelial cells.
[So] Source:Adv Exp Med Biol;581:351-6, 2006.
[Is] ISSN:0065-2598
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Infecções por Coronavirus/virologia
Coronavirus do Rato/metabolismo
Células Epiteliais/virologia
Glicoproteínas de Membrana/metabolismo
Alvéolos Pulmonares/virologia
Proteínas do Envelope Viral/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Infecções por Coronavirus/patologia
Pneumopatias/virologia
Microscopia de Fluorescência
Ratos
Glândulas Salivares/virologia
Glicoproteína da Espícula de Coronavírus
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Membrane Glycoproteins); 0 (Spike Glycoprotein, Coronavirus); 0 (Viral Envelope Proteins)
[Em] Mês de entrada:0612
[Cu] Atualização por classe:161109
[Lr] Data última revisão:
161109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:061014
[St] Status:MEDLINE


  6 / 23 MEDLINE  
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[PMID]:12754115
[Au] Autor:Piao YJ; Xu XJ; Piao ZX
[Ad] Endereço:Department of Histology and Embryology, First Military Medical University, Guangzhou 510515, China. piaoyj@fimmu.com
[Ti] Título:[Ultrastructural observation of rat thymus tissue with coronavirus infection].
[So] Source:Di Yi Jun Yi Da Xue Xue Bao;23(5):414-5, 420, 2003 May.
[Is] ISSN:1000-2588
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To investigate the ultrastructure of rat thymus tissues with rat coronavirus (RCV) infection for clarifying the mechanism responsible for the morphological changes of the cells infected by RCV. METHODS: Routine electron microscopy was performed for observing RCV-infected rat thymus tissues. RESULTS: Following RCV infection, endoplasmic reticulum (ER) pools of different dimensions were observed in the cytoplasm of the thymic epithelial reticular cells, merging subsequently with each other into larger ER lakes filled with particles of mature RCV, or viral inclusion bodies. After germination on the ER membrane, the viruses entered the matrix of the ER lake to mature and were eventually excreted to the extracellular space. The RCV particles were spherical in shape with a diameter of 100-130 nm and two distinct membranes, the outer one being the envelope and the inner one the nuclear capsid to enclose the viroplasm. Between the envelop and nuclear capsid was a electron-lucent middle layer comprising one to two thin membranous structures. Large quantity of short spike-like projections starting from the nucleus capsid penetrated the middle layer and the envelop to reach the glycoprotein coat and formed a corona-like structure. Mature RCV particles were distributed around the ER pools, cytoplasm, and intercellular space, and the RCVs in the endosome/lysosome were devoid of the envelop and nuclear capsid. CONCLUSION: The ER lakes are involved in the maturation of the viruses, and the envelop and nuclear capsid of the virus entering the cells from extracellular space are removed and degraded in the endosome/lysosome. Replications of virus occurs in plasma of the thymic epithelial reticular cells, and no RCV can be detected in the thymocytes.
[Mh] Termos MeSH primário: Infecções por Coronavirus/patologia
Coronavirus do Rato
Timo/ultraestrutura
[Mh] Termos MeSH secundário: Animais
Coronavirus do Rato/isolamento & purificação
Coronavirus do Rato/ultraestrutura
Retículo Endoplasmático/ultraestrutura
Microscopia Eletrônica
Ratos
Ratos Wistar
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:0404
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:030520
[St] Status:MEDLINE


  7 / 23 MEDLINE  
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[PMID]:12022389
[Au] Autor:Besselsen DG; Wagner AM; Loganbill JK
[Ad] Endereço:Department of University Animal Care, The University of Arizona, Tucson 85721-0101, USA.
[Ti] Título:Detection of rodent coronaviruses by use of fluorogenic reverse transcriptase-polymerase chain reaction analysis.
[So] Source:Comp Med;52(2):111-6, 2002 Apr.
[Is] ISSN:1532-0820
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Reverse transcriptase-polymerase chain reaction (RT-PCR) assays have proved useful for the detection of mouse hepatitis virus (MHV) and rat coronavirus (RCV) in acutely infected animals and contaminated biomaterials. Fluorogenic nuclease RT-PCR assays combine RT-PCR with an internal fluorogenic hybridization probe, thereby eliminating post-PCR processing and potentially enhancing specificity. Consequently, a fluorogenic nuclease RT-PCR assay specific for rodent coronaviruses was developed. Primer and probe sequences were selected from the viral genome segment that encodes the membrane (M) protein that is highly conserved among rodent coronaviruses. Use of the fluorogenic nuclease RT-PCR detected all strains of MHV and RCV that were evaluated, but did not detect other RNA viruses that naturally infect rodents. Use of the assay detected as little as two femtograms of in vitro transcribed RNA generated from cloned amplicon, and when compared directly with mouse antibody production tests, had similar sensitivity at detecting MHV-A59 in infected cell culture lysates. Finally, use of the assay detected coronavirus RNA in tissues, cage swipes, and feces obtained from mice experimentally infected with MHV, and in tissues and cage swipes obtained from rats naturally infected with RCV. These results indicate that the fluorogenic nuclease RT-PCR assay should provide a potentially high-throughput, PCR-based method to detect rodent coronaviruses in infected rodents and contaminated biological materials.
[Mh] Termos MeSH primário: Animais de Laboratório/virologia
Infecções por Coronavirus/veterinária
Coronavirus do Rato/isolamento & purificação
Vírus da Hepatite Murina/isolamento & purificação
Reação em Cadeia da Polimerase/métodos
Doenças dos Roedores/virologia
[Mh] Termos MeSH secundário: Animais
Bioensaio/métodos
Linhagem Celular
Infecções por Coronavirus/virologia
Coronavirus do Rato/genética
Coronavirus do Rato/metabolismo
Corantes Fluorescentes/metabolismo
Camundongos
Vírus da Hepatite Murina/genética
Vírus da Hepatite Murina/metabolismo
Ratos
Ratos Sprague-Dawley
Sensibilidade e Especificidade
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (Fluorescent Dyes)
[Em] Mês de entrada:0301
[Cu] Atualização por classe:071114
[Lr] Data última revisão:
071114
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:020523
[St] Status:MEDLINE


  8 / 23 MEDLINE  
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[PMID]:10551452
[Au] Autor:Compton SR; Smith AL; Gaertner DJ
[Ad] Endereço:Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-8016, USA.
[Ti] Título:Comparison of the pathogenicity in rats of rat coronaviruses of different neutralization groups.
[So] Source:Lab Anim Sci;49(5):514-8, 1999 Oct.
[Is] ISSN:0023-6764
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND PURPOSE: Rat coronaviruses (RCVs) are common natural pathogens of rats that cause clinical illness, necrosis, and inflammation of respiratory, salivary, and lacrimal organs. The aim of the study was to determine whether antigenically different strains of RCV vary in their pathogenic potential in rats. METHODS: Neutralization groups were identified by use of RCV strain-specific antisera. Sprague Dawley rats were inoculated oronasally with RCV-SDA, RCV-BCMM, or RCV-W. Histologic examination, immunohistochemical analysis, and reverse transcriptase-polymerase chain reaction analysis were performed on tissues from infected rats. RESULTS: Clinical illness was not evident in any of the inoculated rats. The RCV-SDA strain caused mild lesions in the exorbital gland of one rat. The RCV-BCMM strain caused severe lesions in the Harderian and parotid glands and mild lesions in the exorbital glands, lungs, and nasal mucosa. The RCV-W strain caused severe lesions in the Harderian, exorbital, and parotid glands and mild lesions in the submandibular glands, lungs, and nasal mucosa. The RNA concentration was highest in the Harderian, parotid, and exorbital glands of RCV-BCMM- and RCV-W-infected rats at postinoculation day 7. CONCLUSIONS: Although RCV-SDA, RCV-BCMM, and RCV-W caused different degrees and patterns of lesions, neutralization groups are not useful for predicting the pathogenic potential of a new RCV isolate.
[Mh] Termos MeSH primário: Infecções por Coronavirus/veterinária
Coronavirus do Rato/patogenicidade
Doenças dos Roedores/virologia
[Mh] Termos MeSH secundário: Animais
Antígenos Virais/análise
Infecções por Coronavirus/virologia
Coronavirus do Rato/classificação
Coronavirus do Rato/genética
Glândula de Harder/virologia
Pulmão/microbiologia
Mucosa Nasal/virologia
Glândula Parótida/virologia
RNA Viral/análise
Ratos
Ratos Sprague-Dawley
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Glândula Submandibular/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, Viral); 0 (RNA, Viral)
[Em] Mês de entrada:9912
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:991107
[St] Status:MEDLINE


  9 / 23 MEDLINE  
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[PMID]:10551451
[Au] Autor:Compton SR; Vivas-Gonzalez BE; Macy JD
[Ad] Endereço:Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-8016, USA.
[Ti] Título:Reverse transcriptase polymerase chain reaction-based diagnosis and molecular characterization of a new rat coronavirus strain.
[So] Source:Lab Anim Sci;49(5):506-13, 1999 Oct.
[Is] ISSN:0023-6764
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND PURPOSE: Rat coronaviruses (RCV) are highly infectious and spread rapidly through laboratory rat colonies, causing sneezing, nasal and ocular discharges, photophobia, and cervical swelling. Current diagnostic methods include serologic testing and histologic examination. During a recent rat coronavirus outbreak, we tested a rapid, noninvasive method of RCV diagnosis that involved use of reverse transcriptase-polymerase chain reaction (RT-PCR) analysis to detect RCV RNA on cages housing infected rats. METHODS: The RT-PCR was used to detect RCV RNA in tissues from infected rats and on cages housing infected rats and to amplify portions of the RCV N, M, and S genes for molecular characterization. RESULTS: The RT-PCR detected RCV RNA on cages and in tissues from infected rats. The RCV-NJ N gene is most closely related to the MHV-Y N gene. The M proteins of RCV-NJ and RCV-SDA are 99% homologous, and the six RCV S protein fragments are 97 to 100% homologous. CONCLUSIONS: Use of RT-PCR with cage-swab specimens was capable of diagnosing RCV infection in and viral excretion from rats. Additionally, molecular characterization of the N, M, and S genes of RCV-NJ provided baseline information that can be used in performing further epidemiologic studies.
[Mh] Termos MeSH primário: Infecções por Coronavirus/veterinária
Coronavirus do Rato/genética
Proteínas do Nucleocapsídeo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Doenças dos Roedores/virologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Infecções por Coronavirus/diagnóstico
Infecções por Coronavirus/virologia
Enzimas de Restrição do DNA
Estabilidade de Medicamentos
Masculino
Dados de Sequência Molecular
Nucleocapsídeo/química
Nucleocapsídeo/genética
Plásticos
RNA Viral/química
RNA Viral/isolamento & purificação
Ratos
Ratos Sprague-Dawley
Proteínas da Matriz Viral/química
Proteínas da Matriz Viral/genética
Proteínas Virais/química
Proteínas Virais/genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nucleocapsid Proteins); 0 (Plastics); 0 (RNA, Viral); 0 (Viral Matrix Proteins); 0 (Viral Proteins); 0 (nucleocapsid protein, Coronavirus); 108502-71-2 (M protein, Coronavirus); EC 3.1.21.- (DNA Restriction Enzymes)
[Em] Mês de entrada:9912
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:991107
[St] Status:MEDLINE


  10 / 23 MEDLINE  
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[PMID]:8902504
[Au] Autor:Ohsawa K; Watanabe Y; Takakura A; Itoh T; Sato H
[Ad] Endereço:Laboratory Animal Center for Biomedical Research, Nagasaki University School of Medicine, Japan.
[Ti] Título:Replication of rat coronaviruses in intestinal cell line, RCN-9, derived from F344 rats.
[So] Source:Exp Anim;45(4):389-93, 1996 Oct.
[Is] ISSN:1341-1357
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:To examine the susceptibility of the epithelial cell line to rat coronavirus (RCV), we inoculated sialodacryoadenitis virus and Parker's RCV into five cell lines; JTC-19, rat L2, LLC, RCN-9 and LBC cells originating in the lungs, intestines and mammary tumors of rodents. Both RCVs were replicated in LBC and RCN-9 cells, but not in the others. The infectivity titers of both RCVs grown in RCN-9 cells were significantly higher than those in LBC cells in every passage (2.5-3.9 log rate). Both RCVs replicated in LBC cells showed higher tropism to RCN-9 cells than to LBC cells, suggesting that RCN-9 cells are more suitable for the replication of RCVs than LBC cells. The RCN-9 cell line would be useful for the investigation of RCV infection in rodents.
[Mh] Termos MeSH primário: Coronavirus do Rato/crescimento & desenvolvimento
Replicação Viral/fisiologia
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Coronavirus do Rato/isolamento & purificação
Coronavirus do Rato/patogenicidade
Células Epiteliais
Células Gigantes
Intestinos/citologia
Camundongos
Ratos
Ratos Endogâmicos F344
Doenças dos Roedores/virologia
Células Tumorais Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:9702
[Cu] Atualização por classe:161021
[Lr] Data última revisão:
161021
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:961001
[St] Status:MEDLINE



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