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[PMID]:28490584
[Au] Autor:Shang P; Misra S; Hause B; Fang Y
[Ad] Endereço:Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA.
[Ti] Título:A Naturally Occurring Recombinant Enterovirus Expresses a Torovirus Deubiquitinase.
[So] Source:J Virol;91(14), 2017 Jul 15.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Enteroviruses (EVs) are implicated in a wide range of diseases in humans and animals. In this study, a novel enterovirus (enterovirus species G [EVG]) (EVG 08/NC_USA/2015) was isolated from a diagnostic sample from a neonatal pig diarrhea case and identified by using metagenomics and complete genome sequencing. The viral genome shares 75.4% nucleotide identity with a prototypic EVG strain (PEV9 UKG/410/73). Remarkably, a 582-nucleotide insertion, flanked by 3C cleavage sites at the 5' and 3' ends, was found in the 2C/3A junction region of the viral genome. This insertion encodes a predicted protease with 54 to 68% amino acid identity to torovirus (ToV) papain-like protease (PLP) (ToV-PLP). Structural homology modeling predicts that this protease adopts a fold and a catalytic site characteristic of minimal PLP catalytic domains. This structure is similar to those of core catalytic domains of the foot-and-mouth disease virus leader protease and coronavirus PLPs, which act as deubiquitinating and deISGylating (interferon [IFN]-stimulated gene 15 [ISG15]-removing) enzymes on host cell substrates. Importantly, the recombinant ToV-PLP protein derived from this novel enterovirus also showed strong deubiquitination and deISGylation activities and demonstrated the ability to suppress IFN-ß expression. Using reverse genetics, we generated a ToV-PLP knockout recombinant virus. Compared to the wild-type virus, the ToV-PLP knockout mutant virus showed impaired growth and induced higher expression levels of innate immune genes in infected cells. These results suggest that ToV-PLP functions as an innate immune antagonist; enterovirus G may therefore gain fitness through the acquisition of ToV-PLP from a recombination event. Enteroviruses comprise a highly diversified group of viruses. Genetic recombination has been considered a driving force for viral evolution; however, recombination between viruses from two different orders is a rare event. In this study, we identified a special case of cross-order recombination between enterovirus G (order ) and torovirus (order ). This naturally occurring recombination event may have broad implications for other picornaviral and/or nidoviral species. Importantly, we demonstrated that the exogenous ToV-PLP gene that was inserted into the EVG genome encodes a deubiquitinase/deISGylase and potentially suppresses host cellular innate immune responses. Our results provide insights into how a gain of function through genetic recombination, in particular cross-order recombination, may improve the ability of a virus to evade host immunity.
[Mh] Termos MeSH primário: Enzimas Desubiquitinantes/genética
Enterovirus/enzimologia
Enterovirus/genética
Fezes/virologia
Mutagênese Insercional
Torovirus/enzimologia
Torovirus/genética
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Diarreia/veterinária
Enterovirus/isolamento & purificação
Metagenômica
RNA Viral/genética
Recombinação Genética
Análise de Sequência de DNA
Suínos
Doenças dos Suínos/virologia
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral); EC 3.4.19.12 (Deubiquitinating Enzymes)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170512
[St] Status:MEDLINE


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[PMID]:28003490
[Au] Autor:Goldstein SA; Thornbrough JM; Zhang R; Jha BK; Li Y; Elliott R; Quiroz-Figueroa K; Chen AI; Silverman RH; Weiss SR
[Ad] Endereço:Department of Microbiology, Perlman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA.
[Ti] Título:Lineage A Betacoronavirus NS2 Proteins and the Homologous Torovirus Berne pp1a Carboxy-Terminal Domain Are Phosphodiesterases That Antagonize Activation of RNase L.
[So] Source:J Virol;91(5), 2017 Mar 01.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Viruses in the family , within the order , are etiologic agents of a range of human and animal diseases, including both mild and severe respiratory diseases in humans. These viruses encode conserved replicase and structural proteins as well as more diverse accessory proteins, encoded in the 3' ends of their genomes, that often act as host cell antagonists. We previously showed that 2',5'-phosphodiesterases (2',5'-PDEs) encoded by the prototypical , mouse hepatitis virus (MHV), and by Middle East respiratory syndrome-associated coronavirus antagonize the oligoadenylate-RNase L (OAS-RNase L) pathway. Here we report that additional coronavirus superfamily members, including lineage A betacoronaviruses and toroviruses infecting both humans and animals, encode 2',5'-PDEs capable of antagonizing RNase L. We used a chimeric MHV system (MHV ) in which exogenous PDEs were expressed from an MHV backbone lacking the gene for a functional NS2 protein, the endogenous RNase L antagonist. With this system, we found that 2',5'-PDEs encoded by the human coronavirus HCoV-OC43 (OC43; an agent of the common cold), human enteric coronavirus (HECoV), equine coronavirus (ECoV), and equine torovirus Berne (BEV) are enzymatically active, rescue replication of MHV in bone marrow-derived macrophages, and inhibit RNase L-mediated rRNA degradation in these cells. Additionally, PDEs encoded by OC43 and BEV rescue MHV replication and restore pathogenesis in wild-type (WT) B6 mice. This finding expands the range of viruses known to encode antagonists of the potent OAS-RNase L antiviral pathway, highlighting its importance in a range of species as well as the selective pressures exerted on viruses to antagonize it. Viruses in the family include important human and animal pathogens, including the recently emerged viruses severe acute respiratory syndrome-associated coronavirus (SARS-CoV) and Middle East respiratory syndrome-associated coronavirus (MERS-CoV). We showed previously that two viruses within the genus , mouse hepatitis virus (MHV) and MERS-CoV, encode 2',5'-phosphodiesterases (2',5'-PDEs) that antagonize the OAS-RNase L pathway, and we report here that these proteins are furthermore conserved among additional coronavirus superfamily members, including lineage A betacoronaviruses and toroviruses, suggesting that they may play critical roles in pathogenesis. As there are no licensed vaccines or effective antivirals against human coronaviruses and few against those infecting animals, identifying viral proteins contributing to virulence can inform therapeutic development. Thus, this work demonstrates that a potent antagonist of host antiviral defenses is encoded by multiple and diverse viruses within the family , presenting a possible broad-spectrum therapeutic target.
[Mh] Termos MeSH primário: Endorribonucleases/metabolismo
Coronavírus da Síndrome Respiratória do Oriente Médio/enzimologia
Vírus da Hepatite Murina/enzimologia
Diester Fosfórico Hidrolases/fisiologia
Torovirus/enzimologia
Proteínas não Estruturais Virais/fisiologia
[Mh] Termos MeSH secundário: Nucleotídeos de Adenina/química
Sequência de Aminoácidos
Animais
Domínio Catalítico
Linhagem Celular
Sequência Conservada
Cricetinae
Ativação Enzimática
Macrófagos/virologia
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Oligorribonucleotídeos/química
Diester Fosfórico Hidrolases/química
Proteínas não Estruturais Virais/química
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adenine Nucleotides); 0 (Oligoribonucleotides); 0 (Viral Nonstructural Proteins); 61172-40-5 (2',5'-oligoadenylate); EC 3.1.- (Endoribonucleases); EC 3.1.26.- (2-5A-dependent ribonuclease); EC 3.1.4.- (Phosphoric Diester Hydrolases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161223
[St] Status:MEDLINE


  3 / 89 MEDLINE  
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[PMID]:27936566
[Au] Autor:Khedri Z; Xiao A; Yu H; Landig CS; Li W; Diaz S; Wasik BR; Parrish CR; Wang LP; Varki A; Chen X
[Ad] Endereço:Glycobiology Research and Training Center, University of California , San Diego, California 92093, United States.
[Ti] Título:A Chemical Biology Solution to Problems with Studying Biologically Important but Unstable 9-O-Acetyl Sialic Acids.
[So] Source:ACS Chem Biol;12(1):214-224, 2017 Jan 20.
[Is] ISSN:1554-8937
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:9-O-Acetylation is a common natural modification on sialic acids (Sias) that terminate many vertebrate glycan chains. This ester group has striking effects on many biological phenomena, including microbe-host interactions, complement action, regulation of immune responses, sialidase action, cellular apoptosis, and tumor immunology. Despite such findings, 9-O-acetyl sialoglycoconjugates have remained largely understudied, primarily because of marked lability of the 9-O-acetyl group to even small pH variations and/or the action of mammalian or microbial esterases. Our current studies involving 9-O-acetylated sialoglycans on glycan microarrays revealed that even the most careful precautions cannot ensure complete stability of the 9-O-acetyl group. We now demonstrate a simple chemical biology solution to many of these problems by substituting the oxygen atom in the ester with a nitrogen atom, resulting in sialic acids with a chemically and biologically stable 9-N-acetyl group. We present an efficient one-pot multienzyme method to synthesize a sialoglycan containing 9-acetamido-9-deoxy-N-acetylneuraminic acid (Neu5Ac9NAc) and compare it to the one with naturally occurring 9-O-acetyl-N-acetylneuraminic acid (Neu5,9Ac ). Conformational resemblance of the two molecules was confirmed by computational molecular dynamics simulations. Microarray studies showed that the Neu5Ac9NAc-sialoglycan is a ligand for viruses naturally recognizing Neu5,9Ac , with a similar affinity but with much improved stability in handling and study. Feeding of Neu5Ac9NAc or Neu5,9Ac to mammalian cells resulted in comparable incorporation and surface expression as well as binding to 9-O-acetyl-Sia-specific viruses. However, cells fed with Neu5Ac9NAc remained resistant to viral esterases and showed a slower turnover. This simple approach opens numerous research opportunities that have heretofore proved intractable.
[Mh] Termos MeSH primário: Ácidos Siálicos/metabolismo
[Mh] Termos MeSH secundário: Acetilação
Antígenos CD/metabolismo
Linhagem Celular
Membrana Celular/metabolismo
Glicosilação
Hemaglutininas Virais/metabolismo
Seres Humanos
Ligantes
Análise em Microsséries
Conformação Molecular
Simulação de Dinâmica Molecular
Oligonucleotídeos/síntese química
Oligonucleotídeos/química
Oligonucleotídeos/metabolismo
Lectina 2 Semelhante a Ig de Ligação ao Ácido Siálico/metabolismo
Lectinas Semelhantes a Imunoglobulina de Ligação ao Ácido Siálico/metabolismo
Ácidos Siálicos/síntese química
Ácidos Siálicos/química
Torovirus/enzimologia
Proteínas Virais de Fusão/metabolismo
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (CD22 protein, human); 0 (Hemagglutinins, Viral); 0 (Ligands); 0 (Oligonucleotides); 0 (SIGLEC9 protein, human); 0 (Sialic Acid Binding Ig-like Lectin 2); 0 (Sialic Acid Binding Immunoglobulin-like Lectins); 0 (Sialic Acids); 0 (Viral Fusion Proteins); 0 (Viral Proteins); 0 (hemagglutinin esterase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171118
[Lr] Data última revisão:
171118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE
[do] DOI:10.1021/acschembio.6b00928


  4 / 89 MEDLINE  
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[PMID]:27218226
[Au] Autor:Ávila-Pérez G; Rejas MT; Rodríguez D
[Ad] Endereço:Department of Molecular and Cellular Biology, Centro Nacional de Biotecnología, CSIC, C/Darwin 3, 28049, Madrid, Spain.
[Ti] Título:Ultrastructural characterization of membranous torovirus replication factories.
[So] Source:Cell Microbiol;18(12):1691-1708, 2016 Dec.
[Is] ISSN:1462-5822
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Plus-stranded RNA viruses replicate in the cytosol of infected cells, in membrane-bound replication complexes containing the replicase proteins, the viral RNA and host proteins. The formation of the replication and transcription complexes (RTCs) through the rearrangement of cellular membranes is currently being actively studied for viruses belonging to different viral families. In this work, we identified double-membrane vesicles (DMVs) in the cytoplasm of cells infected with the equine torovirus Berne virus (BEV), the prototype member of the Torovirus genus (Coronaviridae family, Nidovirales order). Using confocal microscopy and transmission electron microscopy, we observed a close relationship between the RTCs and the DMVs of BEV. The examination of BEV-infected cells revealed that the replicase proteins colocalize with each other and with newly synthesized RNA and are associated to the membrane rearrangement induced by BEV. However, the double-stranded RNA, an intermediate of viral replication, is exclusively limited to the interior of DMVs. Our results with BEV resemble those obtained with other related viruses in the Nidovirales order, thus providing new evidence to support the idea that nidoviruses share a common replicative structure based on the DMV arranged clusters.
[Mh] Termos MeSH primário: Vesículas Citoplasmáticas/ultraestrutura
Membranas Intracelulares/ultraestrutura
RNA Replicase/genética
RNA Viral/genética
Torovirus/ultraestrutura
Proteínas Virais/genética
Replicação Viral
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Membrana Celular/metabolismo
Membrana Celular/ultraestrutura
Membrana Celular/virologia
Vesículas Citoplasmáticas/metabolismo
Vesículas Citoplasmáticas/virologia
Derme
Células Epiteliais/metabolismo
Células Epiteliais/ultraestrutura
Células Epiteliais/virologia
Fibroblastos/metabolismo
Fibroblastos/ultraestrutura
Fibroblastos/virologia
Regulação Viral da Expressão Gênica
Cavalos
Interações Hospedeiro-Patógeno
Seres Humanos
Membranas Intracelulares/metabolismo
Membranas Intracelulares/virologia
Microscopia Eletrônica de Transmissão
RNA Replicase/metabolismo
RNA de Cadeia Dupla/genética
RNA de Cadeia Dupla/metabolismo
RNA Viral/metabolismo
Transdução de Sinais
Torovirus/genética
Torovirus/metabolismo
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Double-Stranded); 0 (RNA, Viral); 0 (Viral Proteins); EC 2.7.7.48 (RNA Replicase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170518
[Lr] Data última revisão:
170518
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160525
[St] Status:MEDLINE
[do] DOI:10.1111/cmi.12620


  5 / 89 MEDLINE  
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[PMID]:26708248
[Au] Autor:Ito M; Tsuchiaka S; Naoi Y; Otomaru K; Sato M; Masuda T; Haga K; Oka T; Yamasato H; Omatsu T; Sugimura S; Aoki H; Furuya T; Katayama Y; Oba M; Shirai J; Katayama K; Mizutani T; Nagai M
[Ad] Endereço:Ishikawa Nanbu Livestock Hygiene Service Center, Saida, Kanazawa, Ishikawa 920-3101, Japan.
[Ti] Título:Whole genome analysis of Japanese bovine toroviruses reveals natural recombination between porcine and bovine toroviruses.
[So] Source:Infect Genet Evol;38:90-95, 2016 Mar.
[Is] ISSN:1567-7257
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Bovine toroviruses (BToVs), belong to the subfamily Toroviridae within the family Coronaviridae, and are pathogens, causing enteric disease in cattle. In Japan, BToVs are distributed throughout the country and cause gastrointestinal infection of calves and cows. In the present study, complete genome sequences of two Japanese BToVs and partial genome sequences of two Japanese BToVs and one porcine torovirus (PToV) from distant regions in Japan were determined and genetic analyses were performed. Pairwise nucleotide comparison and phylogenetic analyses revealed that Japanese BToVs shared high identity with each other and showed high similarities with BToV Breda1 strain in S, M, and HE coding regions. Japanese BToVs showed high similarities with porcine toroviruses in ORF1a, ORF1b, and N coding regions and the 5' and 3' untranslated regions, suggestive of a natural recombination event. Recombination analyses mapped the putative recombinant breakpoints to the 3' ends of the ORF1b and HE regions. These findings suggest that the interspecies recombinant nature of Japanese BToVs resulted in a closer relationship between BToV Breda1 and PToVs.
[Mh] Termos MeSH primário: Doenças dos Bovinos/virologia
Genoma Viral
Recombinação Genética
Suínos/virologia
Torovirus/genética
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Bovinos
Sequenciamento de Nucleotídeos em Larga Escala
Japão
Dados de Sequência Molecular
Fases de Leitura Aberta
Filogenia
Alinhamento de Sequência
Análise de Sequência de DNA
Doenças dos Suínos
Torovirus/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1610
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151229
[St] Status:MEDLINE


  6 / 89 MEDLINE  
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[PMID]:26616156
[Au] Autor:Tsuchiaka S; Masuda T; Sugimura S; Kobayashi S; Komatsu N; Nagai M; Omatsu T; Furuya T; Oba M; Katayama Y; Kanda S; Yokoyama T; Mizutani T
[Ad] Endereço:The United Graduate School of Veterinary Sciences, Gifu University, Yanagito, Gifu 501-1193, Japan.
[Ti] Título:Development of a novel detection system for microbes from bovine diarrhea by real-time PCR.
[So] Source:J Vet Med Sci;78(3):383-9, 2016 Mar.
[Is] ISSN:1347-7439
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Diarrhea in cattle is one of the most economically costly disorders, decreasing milk production and weight gain. In the present study, we established a novel simultaneous detection system using TaqMan real-time PCR designed as a system for detection of microbes from bovine diarrhea using real-time PCR (referred to as Dembo-PCR). Dembo-PCR simultaneously detects a total of 19 diarrhea-causing pathogens, including viruses, bacteria and protozoa. Specific primer-probe sets were newly designed for 7 pathogens and were synthesized on the basis of previous reports for 12 pathogens. Assays were optimized to react under the same reaction conditions. The PCR efficiency and correlation coefficient (R(2)) of standard curves for each assay were more than 80% and 0.9766, respectively. Furthermore, the sensitivity of Dembo-PCR in fecal sample analysis was measured with feces spiked with target pathogens or synthesized DNA that included specific nucleotide target regions. The resulting limits of detection (LOD) for virus-spiked samples, bacteria and DNA fragments were 0.16-1.6 TCID50 (PFU/reaction), 1.3-13 CFU/reaction and 10-100 copies/reaction, respectively. All reactions showed high sensitivity in pathogen detection. A total of 8 fecal samples, collected from 6 diarrheic cattle, 1 diarrheic calf and 1 healthy cow, were tested using Dembo-PCR to validate the assay's clinical performance. The results revealed that bovine coronavirus had infected all diarrheic adult cattle and that bovine torovirus had infected the diarrheic calf. These results suggest that Dembo-PCR may be a powerful tool for diagnosing infectious agents in cattle diarrhea.
[Mh] Termos MeSH primário: Doenças dos Bovinos/microbiologia
Diarreia/microbiologia
Reação em Cadeia da Polimerase em Tempo Real/veterinária
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos/diagnóstico
Infecções por Coronavirus/diagnóstico
Infecções por Coronavirus/veterinária
Coronavirus Bovino
Diarreia/diagnóstico
Feminino
Torovirus
Infecções por Torovirus/diagnóstico
Infecções por Torovirus/veterinária
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151201
[St] Status:MEDLINE
[do] DOI:10.1292/jvms.15-0552


  7 / 89 MEDLINE  
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[PMID]:26611229
[Au] Autor:Liu X; Zhou Y; Yang F; Liu P; Cai Y; Huang J; Zhu L; Xu Z
[Ad] Endereço:Key Laboratory of Animal Biotechnology Center, College of Veterinary Medicine of Sichuan Agricultural University, Huimin Road 211, Wenjiang district, Sichuan 611134, PR China. Electronic address: abtclxw@126.com.
[Ti] Título:Rapid and sensitive detection of porcine torovirus by a reverse transcription loop-mediated isothermal amplification assay (RT-LAMP).
[So] Source:J Virol Methods;228:103-7, 2016 Feb.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Porcine torovirus (PToV) is associated with swine gastroenteritis, but its pathogenesis is uncertain because there is limited information regarding PToV due to its difficulty to adapt in vitro. This study has developed a rapid one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for the detection of PToV. A set of four primers specific to six regions within the PToV's highly conserved fragment of the M gene was designed for use with the RT-LAMP assay. The RT-LAMP assay was sensitive with a detection limit of 1 × 10(1)copies/µL, which was 100-fold higher than reverse-transcription PCR. No cross-reaction was observed with other similar viruses. A total of 175 clinical specimens were collected from the Sichuan province, and PToV was detected by the established RT-LAMP assay with a positive rate of 39.2% (69/175). This study developed the first rapid, sensitive, simple, cost-effective and accurate method for the detection of PToV. The results show that the RT-LAMP assay is highly feasible in clinical settings.
[Mh] Termos MeSH primário: Técnicas de Amplificação de Ácido Nucleico/métodos
Doenças dos Suínos/virologia
Infecções por Torovirus/veterinária
Torovirus/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Reações Cruzadas
Primers do DNA
Seres Humanos
Limite de Detecção
Técnicas de Amplificação de Ácido Nucleico/economia
RNA Viral
Transcrição Reversa
Sensibilidade e Especificidade
Suínos
Doenças dos Suínos/diagnóstico
Torovirus/genética
Infecções por Torovirus/diagnóstico
Infecções por Torovirus/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA Primers); 0 (RNA, Viral)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151128
[St] Status:MEDLINE


  8 / 89 MEDLINE  
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[PMID]:26268320
[Au] Autor:Lojkic I; Kresic N; Simic I; Bedekovic T
[Ad] Endereço:Department of Virology, Croatian Veterinary Institute, Savska cesta 143, 10000, Zagreb, Croatia. ilojkic@veinst.hr.
[Ti] Título:Detection and molecular characterisation of bovine corona and toroviruses from Croatian cattle.
[So] Source:BMC Vet Res;11:202, 2015 Aug 13.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Bovine coronavirus (BCoV) together with bovine torovirus (BToV), both members of the Coronaviridae family, order Nidovirales are the most common viral enteric pathogens. Although studied separately, their joint occurrence and the molecular diversity in cattle in Croatia have not been investigated. METHODS: A survey is carried out on 101 fecal samples from diarrheic young and adult cattle during the 3-year period from i) one large dairy herd, ii) four small herds and iii) three nasal and paired fecal samples from calves with symptoms of respiratory disease. Samples were submitted to RT-PCR and sequencing for BCoV Nucleocapsid gene, BCoV Spike gene and BToV Spike gene. RESULTS: BCoV was detected in 78.8 % of fecal samples from symptomatic cattle and three nasal and paired fecal samples from calves with respiratory symptoms. BToV was detected in 43.2 % of fecal samples from symptomatic cattle and a fecal sample from calves with respiratory symptoms. Molecular characterisation of those viruses revealed some nucleotide and aminoacid differences in relation to reference strains. CONCLUSIONS: BToV should be regarded as a relevant pathogen for cattle that plays a synergistic role in mixed enteric infections.
[Mh] Termos MeSH primário: Doenças dos Bovinos/virologia
Infecções por Coronavirus/veterinária
Coronavirus Bovino/isolamento & purificação
Infecções por Torovirus/veterinária
Torovirus/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos/epidemiologia
Infecções por Coronavirus/epidemiologia
Infecções por Coronavirus/virologia
Coronavirus Bovino/genética
Croácia/epidemiologia
DNA Complementar/genética
DNA Viral/genética
Diarreia/epidemiologia
Diarreia/veterinária
Diarreia/virologia
Fezes/virologia
Filogenia
Alinhamento de Sequência
Torovirus/genética
Infecções por Torovirus/epidemiologia
Infecções por Torovirus/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (DNA, Viral)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150815
[Lr] Data última revisão:
150815
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150814
[St] Status:MEDLINE
[do] DOI:10.1186/s12917-015-0511-9


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[PMID]:24903213
[Au] Autor:Zhou L; Wei H; Zhou Y; Xu Z; Zhu L; Horne J
[Ti] Título:Molecular epidemiology of Porcine torovirus (PToV) in Sichuan Province, China: 2011-2013.
[So] Source:Virol J;11:106, 2014 Jun 05.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Porcine torovirus (PToV) is a member of the genus Torovirus which is responsible for gastrointestinal disease in both human beings and animals with particular prevalence in youth. Torovirus infections are generally asymptomatic, however, their presence may worsen disease consequences in concurrent infections with other enteric pathogens. METHODS: A total of 872 diarrheic fecal samples from pigs of different ages were collected from 12 districts of Sichuan Province in the southwest of China. RT-PCR was done with PToV S gene specific primers to detect the presence of PToV positive samples. M gene specific primers were used with the PToV positive samples and the genes were sequenced. A phylogenetic tree was constructed based on the M gene nucleotide sequences from the 19 selected novel Sichuan strains and 21 PToV and BToV M gene sequences from GenBank. RESULTS: A total of 331 (37.96%, 331/872) samples were found to be positive for PToV and the highest prevalence was observed in piglets aged from 1 to 3 weeks old. Through phylogenetic inference the 40 PToV M gene containing sequences were placed into two genotypes (I & II). The 19 novel Sichuan strains of genotype I showed strong correlations to two Korean gene sequences (GU-07-56-11 and GU-07-56-22). Amino-acid sequence analysis of the 40 PToV M gene strains revealed that the M gene protein was highly conserved. CONCLUSIONS: This study uncovered the presence of PToV in Sichuan Province, and demonstrated the need for continuous surveillance PToV of epidemiology.
[Mh] Termos MeSH primário: Doenças dos Suínos/epidemiologia
Doenças dos Suínos/virologia
Infecções por Torovirus/veterinária
Torovirus/classificação
Torovirus/genética
[Mh] Termos MeSH secundário: Animais
China/epidemiologia
Análise por Conglomerados
Diarreia/epidemiologia
Diarreia/veterinária
Diarreia/virologia
Fezes/virologia
Epidemiologia Molecular
Dados de Sequência Molecular
Filogenia
RNA Viral/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Análise de Sequência de DNA
Homologia de Sequência
Suínos
Torovirus/isolamento & purificação
Infecções por Torovirus/epidemiologia
Infecções por Torovirus/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:150805
[Lr] Data última revisão:
150805
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140607
[St] Status:MEDLINE
[do] DOI:10.1186/1743-422X-11-106


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[PMID]:24420162
[Au] Autor:Gülaçti I; Isidan H; Sözdutmaz I
[Ad] Endereço:The Pendik Veterinary Control and Research Institute, 34890, Istanbul, Turkey.
[Ti] Título:Detection of bovine torovirus in fecal specimens from calves with diarrhea in Turkey.
[So] Source:Arch Virol;159(7):1623-7, 2014 Jul.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Bovine torovirus (BToV), a member of the family Coronaviridae, is an established gastrointestinal infectious agent in cattle. In this study, we performed a survey to detect BToV in Turkey between 2009 and 2011 using 235 fecal samples from neonatal calves with diarrhea that were analyzed by the nested reverse transcription (RT) PCR method using primers located in the consensus sequences of the BToV membrane (M) gene. The BToV M gene was detected in 4.7 % (11/235) of the samples using the nested RT-PCR method. The nucleotide sequences of partial M fragments from the BToV isolates, including the newly identified Turkish isolates, showed more than 96 % identity. The result indicates that BToV is one of the pathogens that contribute to neonatal calf diarrhea cases in Turkey.
[Mh] Termos MeSH primário: Doenças dos Bovinos/virologia
Diarreia/veterinária
Fezes/virologia
Infecções por Torovirus/veterinária
Torovirus/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos/epidemiologia
Diarreia/virologia
Filogenia
Torovirus/genética
Infecções por Torovirus/epidemiologia
Infecções por Torovirus/virologia
Turquia/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1408
[Cu] Atualização por classe:140626
[Lr] Data última revisão:
140626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140115
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-014-1977-7



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