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[PMID]:28044194
[Au] Autor:Dong X; Liu S; Zhu L; Wan X; Liu Q; Qiu L; Zou P; Zhang Q; Huang J
[Ad] Endereço:Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.
[Ti] Título:Complete genome sequence of an isolate of a novel genotype of yellow head virus from Fenneropenaeus chinensis indigenous in China.
[So] Source:Arch Virol;162(4):1149-1152, 2017 Apr.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Genotype 8 of yellow head virus (YHV-8) was identified recently, but the complete genome sequence of this new genotype has not been reported. In this study, the complete genome of YHV-8 isolate 20120706 collected from Hebei Province of China in 2012 was sequenced. It was found to be 26,769 nucleotides (nt) in length, including a 20,060-nt open reading frame 1 (ORF1), a 435-nt ORF2, and a 4971-nt ORF3. Isolate 20120706 shared 79.7-83.9% nucleotide sequence identity with all seven of the complete genome sequences of YHV that have been reported so far. The topology of a phylogenetic tree constructed based on the ORF1b region clearly showed that strain 20120706, together with five other YHV-8 strains isolated in China, represents a new genotype of YHV. This is the first report of the complete genome sequence of a YHV-8 isolate, and the 20120706 isolate will be useful for further analysis of the epidemiology and evolution of YHV-8.
[Mh] Termos MeSH primário: Genoma Viral
Penaeidae/virologia
Roniviridae/genética
Roniviridae/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
China
Genótipo
Dados de Sequência Molecular
Fases de Leitura Aberta
Filogenia
Roniviridae/classificação
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170315
[Lr] Data última revisão:
170315
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170104
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-016-3203-2


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[PMID]:27221155
[Au] Autor:Yang HL; Qiu L; Liu Q; Wan XY; Liu S; Zhu LL; Yang B; Zhang QL; Huang J
[Ad] Endereço:Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Laboratory for Marine Fisheries and Aquaculture, Qingdao National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.
[Ti] Título:A novel method of real-time reverse-transcription loop-mediated isothermal amplification developed for rapid and quantitative detection of a new genotype (YHV-8) of yellow head virus.
[So] Source:Lett Appl Microbiol;63(2):103-10, 2016 Aug.
[Is] ISSN:1472-765X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: A new genotype of yellow head virus (YHV), designated as YHV-8, was found in farmed shrimp Fenneropenaeus chinensis suffering suspectedly from EMS/AHPNS (early mortality disease/acute hepatopancreatic necrosis disease) in China in 2012. In this study, a one-step, real-time reverse-transcription loop-mediated isothermal amplification (rRT-LAMP) assay was developed for better detection of both genotypes of YHV-1 and YHV-8. A set of six specific primers was successfully designed targeting a conserved region of the YHV genome. The LAMP reaction was optimized to contain 8 mmol l(-1) Mg(2+) and 1·4 mmol l(-1) dNTPs, and to be performed at 58°C for 60 min. The detection sensitivity of the rRT-LAMP method was as low as 7 × 10(0)  copies per reaction. The specificity of the method was validated by the absence of any cross-reaction with the RNA samples extracted from other shrimp viruses (Taura syndrome virus, white spot syndrome virus, infectious hypodermal and haematopoietic necrosis virus, hepatopancreatic parvovirus) and specific pathogen-free (SPF) shrimp. The resulting standard curves showed high correlation coefficient values. Furthermore, the test of farm samples showed that YHV was detected in three of 111 Litopenaeus vannamei, six of eight Fenneropenaeus chinensis, five of 19 Macrobrachium rosenbergii and none of the nine Marsupenaeus japonicus. These results suggest that this assay is applicable widely as a new rapid and sensitive detection method in the research of YHV. SIGNIFICANCE AND IMPACT OF THE STUDY: In this study, we designate a new genotype of yellow head virus (YHV) as YHV genotype 8 (YHV-8) which was detected in diseased shrimp in China. A rapid, sensitive and specific rRT-LAMP detecting method for both YHV-8 and YHV-1 has been established. It is anticipated that this novel assay will be instrumental for diagnosis and surveillance of the virulent genotypes of YHV.
[Mh] Termos MeSH primário: Hepatopâncreas/virologia
Técnicas de Amplificação de Ácido Nucleico/métodos
Penaeidae/virologia
Roniviridae/genética
[Mh] Termos MeSH secundário: Animais
China
Primers do DNA
Genótipo
Hepatopâncreas/patologia
Transcrição Reversa
Roniviridae/classificação
Roniviridae/isolamento & purificação
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160526
[St] Status:MEDLINE
[do] DOI:10.1111/lam.12591


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[PMID]:27140871
[Au] Autor:Sanitt P; Apiratikul N; Niyomtham N; Yingyongnarongkul BE; Assavalapsakul W; Panyim S; Udomkit A
[Ad] Endereço:Institute of Molecular Biosciences, Mahidol University, Salaya Campus, Nakhon Pathom 73170, Thailand.
[Ti] Título:Cholesterol-based cationic liposome increases dsRNA protection of yellow head virus infection in Penaeus vannamei.
[So] Source:J Biotechnol;228:95-102, 2016 Jun 20.
[Is] ISSN:1873-4863
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Protection of shrimp from yellow head virus (YHV) infection has been demonstrated by injection and oral delivery of dsRNA-YHV protease gene (dsYHV) or shrimp endogenous gene (dsRab7). However, to achieve complete viral suppression and to prolong dsRNA activity, the development of an effective dsRNA delivery system is required. In this study, four cationic liposomes were synthesized and tested for their ability to increase dsRNA efficiency. The results demonstrated that entrapping dsYHV in a cholesterol-based cationic liposome gave the best protection against YHV infection when compared with other cationic lipids. The cholesterol-based cationic liposome-dsYHV (Chol-dsYHV) complex conferred YHV protection in a dose-dependent manner. Injection with Chol-dsYHV at 0.05µg dsYHV/g shrimp could give comparable level of YHV protection to the injection with 1.25µg naked dsYHV/g shrimp. The shrimp injected with Chol- dsYHV at 1.25µg dsRNA/g shrimp showed only 50% mortality at 60days post injection whereas the naked dsYHV at the same concentration gave 90% mortality. Thus, the liposome-entrapped dsYHV could lower an effective dsRNA concentration in viral protection and prolong dsRNA activity. In addition, encapsulating dsRab7 in the cholesterol-based cationic liposome could protect the dsRab7 from enzymatic digestion, and continuous feeding the shrimp with the diet formulated with the liposome-entrapped dsRab7 for 4days in the total of 960µg dsRab7/g shrimp could enhance YHV protection efficiency compared with the naked dsRab7. Our studies reveal that cholesterol-based cationic liposome is a promising dsRNA carrier to enhance dsRNA efficiency in both injection and oral delivery systems.
[Mh] Termos MeSH primário: Colesterol/química
Lipossomos/farmacologia
Infecções por Nidovirales
Penaeidae/virologia
RNA de Cadeia Dupla/metabolismo
Roniviridae/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Lipossomos/administração & dosagem
Infecções por Nidovirales/tratamento farmacológico
Infecções por Nidovirales/prevenção & controle
Infecções por Nidovirales/veterinária
Infecções por Nidovirales/virologia
Interferência de RNA/efeitos dos fármacos
Roniviridae/genética
Replicação Viral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Liposomes); 0 (RNA, Double-Stranded); 97C5T2UQ7J (Cholesterol)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170806
[Lr] Data última revisão:
170806
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160504
[St] Status:MEDLINE


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[PMID]:26384157
[Au] Autor:Havanapan PO; Taengchaiyaphum S; Ketterman AJ; Krittanai C
[Ad] Endereço:Institute of Molecular Biosciences, Mahidol University, Salaya Campus, Salaya, Nakhonpathom 73170, Thailand.
[Ti] Título:Yellow head virus infection in black tiger shrimp reveals specific interaction with granule-containing hemocytes and crustinPm1 as a responsive protein.
[So] Source:Dev Comp Immunol;54(1):126-36, 2016 Jan.
[Is] ISSN:1879-0089
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Yellow head virus (YHV) causes acute infections and mass mortality in black tiger shrimp culture. Our study aims to investigate molecular interaction between YHV and circulating hemocytes of Penaeus monodon at early infection. Total shrimp hemocytes were isolated by Percoll gradient centrifugation and identified by flow cytometric analysis. At least three types of hemocyte cells were identified as hyaline, semi-granular, and granular hemocytes. Experimental infection of YHV in shrimp culture demonstrated drastic changes in total and each hemocyte cell counts. Immunohistochemistry analysis demonstrated interaction and replication of YHV mainly with the granule-containing hemocytes and little to none in hyaline cell. These granule-containing hemocytes are proposed to be YHV targets providing the first line of defense to viral infection. Protein expression profiling of granule-containing hemocytes revealed several immune-responsive proteins including antimicrobial protein crustins (crustinPm1 and crustinPm4), alpha-2-macroglobulin, and kazal-type serine proteinase inhibitor. During an early phase of YHV infection at 6 hpi crustinPm1 illustrated a significant increase of mRNA and protein expression level in plasma. The results suggest that an antimicrobial crustinPm1 may participate in shrimp defense mechanism against YHV, especially on the granule-containing hemocytes.
[Mh] Termos MeSH primário: Peptídeos Catiônicos Antimicrobianos/imunologia
Hemócitos/imunologia
Penaeidae/imunologia
Penaeidae/virologia
Roniviridae
[Mh] Termos MeSH secundário: Animais
Western Blotting
Grânulos Citoplasmáticos/imunologia
Citometria de Fluxo
Perfilação da Expressão Gênica
Processamento de Imagem Assistida por Computador
Imuno-Histoquímica
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antimicrobial Cationic Peptides); 0 (crustinPm1 protein, Penaeus monodon)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151102
[Lr] Data última revisão:
151102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150919
[St] Status:MEDLINE


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[PMID]:26434714
[Au] Autor:Visetnan S; Supungul P; Tang S; Hirono I; Tassanakajon A; Rimphanitchayakit V
[Ad] Endereço:Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phyathai Road, Bangkok 10330, Thailand.
[Ti] Título:YHV-responsive gene expression under the influence of PmRelish regulation.
[So] Source:Fish Shellfish Immunol;47(1):572-81, 2015 Nov.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In animals, infection by Gram-negative bacteria and certain viruses activates the Imd signaling pathway wherein the a NF-κB transcription factor, Relish, is a key regulatory protein for the synthesis of antimicrobial proteins. Infection by yellow head virus (YHV) activates the Imd pathway. To investigate the expression of genes involved in YHV infection and under the influence of PmRelish regulation, RNA interference and suppression subtractive hybridization (SSH) are employed. The genes in forward library expressed in shrimp after YHV infection and under the activity of PmRelish were obtained by subtracting the cDNAs from YHV-infected and PmRelish-knockdown shrimp with cDNAs from YHV-infected shrimp. Opposite subtraction gave a reverse library whereby an alternative set of genes under YHV infection and no PmRelish expression were obtained. Nucleotide sequences of 252 and 99 cDNA clones from the forward and reverse libraries, respectively, were obtained and annotated through blast search against the GenBank sequences. Genes involved in defense and homeostasis were abundant in both libraries, 31% and 23% in the forward and reverse libraries, respectively. They were predominantly antimicrobial proteins, proteinases and proteinase inhibitors. The expression of antimicrobial protein genes, ALFPm3, crustinPm1, penaeidin3 and penaeidin5 were tested under PmRelish silencing and Gram-negative bacterium Vibrio harveyi infection. Together with the results using YHV infection previously reported, the expression of penaeidin5 and also penaeidin3 but not ALFPm3 and crustinPm1 were under the regulation of PmRelish in the Imd pathway.
[Mh] Termos MeSH primário: Proteínas de Artrópodes/genética
Regulação da Expressão Gênica
NF-kappa B/genética
Penaeidae/genética
Roniviridae/fisiologia
Vibrio/fisiologia
[Mh] Termos MeSH secundário: Animais
Proteínas de Artrópodes/metabolismo
NF-kappa B/metabolismo
Penaeidae/metabolismo
Penaeidae/microbiologia
Penaeidae/virologia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Arthropod Proteins); 0 (NF-kappa B)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151103
[Lr] Data última revisão:
151103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151006
[St] Status:MEDLINE


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[PMID]:26290511
[Au] Autor:Mohr PG; Moody NJ; Hoad J; Williams LM; Bowater RO; Cummins DM; Cowley JA; StJ Crane M
[Ad] Endereço:CSIRO Australian Animal Health Laboratory, Geelong, VIC 3220, Australia.
[Ti] Título:New yellow head virus genotype (YHV7) in giant tiger shrimp Penaeus monodon indigenous to northern Australia.
[So] Source:Dis Aquat Organ;115(3):263-8, 2015 Aug 20.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In 2012, giant tiger shrimp Penaeus monodon originally sourced from Joseph Bonaparte Gulf in northern Australia were examined in an attempt to identify the cause of elevated mortalities among broodstock at a Queensland hatchery. Nucleic acid extracted from ethanol-fixed gills of 3 individual shrimp tested positive using the OIE YHV Protocol 2 RT-PCR designed to differentiate yellow head virus (YHV1) from gill-associated virus (GAV, synonymous with YHV2) and the OIE YHV Protocol 3 RT-nested PCR designed for consensus detection of YHV genotypes. Sequence analysis of the 794 bp (Protocol 2) and 359 bp (Protocol 3) amplicons from 2 distinct regions of ORF1b showed that the yellow-head-complex virus detected was novel when compared with Genotypes 1 to 6. Nucleotide identity on the Protocol 2 and Protocol 3 ORF1b sequences was highest with the highly pathogenic YHV1 genotype (81 and 87%, respectively) that emerged in P. monodon in Thailand and lower with GAV (78 and 82%, respectively) that is enzootic to P. monodon inhabiting eastern Australia. Comparison of a longer (725 bp) ORF1b sequence, spanning the Protocol 3 region and amplified using a modified YH30/31 RT-nPCR, provided further phylogenetic evidence for the virus being distinct from the 6 described YHV genotypes. The virus represents a unique seventh YHV genotype (YHV7). Despite the mortalities observed, the role of YHV7 remains unknown.
[Mh] Termos MeSH primário: Genótipo
Penaeidae/virologia
Roniviridae/genética
[Mh] Termos MeSH secundário: Animais
Austrália
Interações Hospedeiro-Patógeno
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1511
[Cu] Atualização por classe:160818
[Lr] Data última revisão:
160818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150821
[St] Status:MEDLINE
[do] DOI:10.3354/dao02894


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[PMID]:26203887
[Au] Autor:Maikaeo L; Chotigeat W; Mahabusarakam W
[Ad] Endereço:Department of Molecular biology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
[Ti] Título:Emilia sonchifolia extract activity against white spot syndrome virus and yellow head virus in shrimp cell cultures.
[So] Source:Dis Aquat Organ;115(2):157-64, 2015 Jul 23.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Emilia sonchifolia (L.) DC is a plant used in traditional medicine to treat several viral and bacterial diseases. The antiviral activities of selected Sephadex LH-20 column fractions and HPLC subfractions of an acetone extract of E. sonchifolia leaves were determined in shrimp Penaeus merguiensis primary lymphoid cells infected with either white spot syndrome virus (WSSV) or yellow head virus (YHV). WSSV and YHV replication was quantified using quantitative real-time PCR tests targeted to the VP19 and ORF1b gene transcripts, respectively. In lymphoid organ cells exposed to 100 µg ml⁻¹ of either the Sephadex fraction F14 or the HPLC F14 subfraction SF4, both fractions caused reduced replication, but YHV replication was reduced only by SF4. In the asthiazolyl blue mitochondrial enzyme activity assays to assess extract cytotoxicity, >60% of primary lymphoid organ cells remained viable following exposure to 100 µg ml⁻¹ of either F14 or SF4. GC-MS analysis of the HPLC F14 subfraction SF4 showed that it contained 2,4-di-tert-butylphenol. This study is the first to show that E. sonchifolia leaf extracts might be useful as bioactive agents to protect shrimp against viruses such as WSSV and YHV.
[Mh] Termos MeSH primário: Antivirais/farmacologia
Asteraceae/química
Extratos Vegetais/farmacologia
Roniviridae/efeitos dos fármacos
Vírus 1 da Síndrome da Mancha Branca/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Antivirais/química
Células Cultivadas
Penaeidae/citologia
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Plant Extracts)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:150724
[Lr] Data última revisão:
150724
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150724
[St] Status:MEDLINE
[do] DOI:10.3354/dao02891


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[PMID]:25463289
[Au] Autor:Visetnan S; Supungul P; Hirono I; Tassanakajon A; Rimphanitchayakit V
[Ad] Endereço:Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phyathai Road, Bangkok 10330, Thailand.
[Ti] Título:Activation of PmRelish from Penaeus monodon by yellow head virus.
[So] Source:Fish Shellfish Immunol;42(2):335-44, 2015 Feb.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Humoral innate immune response against pathogenic infection is partly responsible by the Imd pathway in which a transcription factor Relish relays the infection signals to the nuclei for the expression of antimicrobial proteins. A PmRelish gene which encoded a protein of 1195 amino acids was cloned. The PmRelish was constitutively expressed in all tissues tested and mostly up-regulated upon YHV infection. In hemocytes, the PmRelish expression was up-regulated upon Vibrio harveyi, yellow head virus (YHV) and white spot syndrome virus (WSSV) challenges. Using dsRNA silencing of PmRelish gene, it was shown that the expression of penaeidin5 but not anti-lipopolysaccharide factor ALFPm3, crustinPm1 and penaeidin3 was under the regulation of Imd pathway. Under PmRelish silencing, the shrimp were more susceptible to infection by YHV with the 50% survival rate reduced from about 72 h to 42 h. The PmRelish was detected in the cytoplasm of all the hemocytes from both uninfected and YHV-infected shrimp. The accumulation of activated PmRelish in the nuclei was not clearly observed but the activated PmRelish was detected in the YHV-infected hemocytes by Western blot analysis. Thus, the PmRelish and, hence, the Imd pathway respond to the YHV infection.
[Mh] Termos MeSH primário: Proteínas de Artrópodes/genética
Penaeidae/genética
Penaeidae/virologia
Roniviridae/fisiologia
Vibrio/fisiologia
Vírus 1 da Síndrome da Mancha Branca/fisiologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Proteínas de Artrópodes/metabolismo
Sequência de Bases
Western Blotting
Hemócitos/metabolismo
Dados de Sequência Molecular
Especificidade de Órgãos
Penaeidae/metabolismo
Interferência de RNA
RNA de Cadeia Dupla/metabolismo
Fatores de Transcrição
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Arthropod Proteins); 0 (RNA, Double-Stranded); 0 (Transcription Factors)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:150105
[Lr] Data última revisão:
150105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE


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[PMID]:25463288
[Au] Autor:Leebonoi W; Sukthaworn S; Panyim S; Udomkit A
[Ad] Endereço:Institute of Molecular Biosciences, Mahidol University, Salaya Campus, Nakhon Pathom, 73170, Thailand.
[Ti] Título:A novel gonad-specific Argonaute 4 serves as a defense against transposons in the black tiger shrimp Penaeus monodon.
[So] Source:Fish Shellfish Immunol;42(2):280-8, 2015 Feb.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Argonaute is a key protein of the small-RNA guided gene regulation process. The Argonaute family is generally divided into two subfamilies; AGO and PIWI. In this study, a cDNA encoding a novel type of Argonaute (PmAgo4) in the black tiger shrimp Penaeus monodon was identified and characterized. PmAgo4 cDNA contained an open reading frame of 2433 nucleotides that can be translated into a deduced amino acid with the conserved PAZ and PIWI domains. PmAgo4 was phylogenetically clustered with the AGO subfamily while exhibited a gonad-specific expression pattern similar to that of proteins in the PIWI subfamily. The expression of PmAgo4 did not change significantly in response to either double-stranded RNA or yellow head virus injection suggesting that PmAgo4 may not be the main AGO proteins that play a role in dsRNA-mediated gene silencing or antiviral defense. Interestingly, PmAgo4 appeared to participate in the control of transposons since the activation of both DNA transposon and retrotransposon was detected in the testis of PmAgo4-knockdown shrimp. Our study thus provided the first evidence for an unusual type of the AGO proteins that was predominantly expressed in shrimp gonad and implication of its role in protecting the shrimp genome against an invasion of transposons.
[Mh] Termos MeSH primário: Proteínas Argonauta/genética
Proteínas de Artrópodes/genética
Elementos de DNA Transponíveis
Regulação da Expressão Gênica
Penaeidae/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Proteínas Argonauta/metabolismo
Proteínas de Artrópodes/metabolismo
Sequência de Bases
Clonagem Molecular
DNA Complementar/genética
DNA Complementar/metabolismo
Gônadas/metabolismo
Dados de Sequência Molecular
Penaeidae/imunologia
Penaeidae/metabolismo
Penaeidae/virologia
Filogenia
Reação em Cadeia da Polimerase
RNA de Cadeia Dupla/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Roniviridae/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Argonaute Proteins); 0 (Arthropod Proteins); 0 (DNA Transposable Elements); 0 (DNA, Complementary); 0 (RNA, Double-Stranded); 0 (RNA, Messenger)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:150105
[Lr] Data última revisão:
150105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE


  10 / 76 MEDLINE  
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[PMID]:25172109
[Au] Autor:Havanapan PO; Taengchaiyaphum S; Bourchookarn A; Ketterman AJ; Krittanai C
[Ad] Endereço:Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhonpathom, Thailand.
[Ti] Título:Yellow head virus binding to cell surface proteins from Penaeus monodon hemocytes.
[So] Source:Fish Shellfish Immunol;41(2):126-36, 2014 Dec.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Our previous data revealed that viral particles of yellow head virus (YHV) specifically interacted with granule-containing hemocytes. After isolation of targeted hemocytes, biotinylation was performed using Biotin-NSH-LC. Biotinylated protein was extracted and separated by 2-D PAGE. Electro-transferred proteins on a nitrocellulose membrane were probed with streptavidin-HRP complex to detect biotinylated proteins. The data from 2-D PAGE combined with affinity pull down purification revealed 8 and 6 biotinylated proteins specific to hyaline and granule containing hemocytes, respectively. Four proteins were found in common for both two hemocytes. The majority of proteins detected in granular hemocytes are membrane-associated proteins and immune-related proteins such as alpha-2-macroglobulin (A2M), kazal-type serine protease inhibitor (SPI) and crustin. CrustinPm1 was found to bind to YHV as shown with biotinylation pull-down assay and confirmed with two-dimensional virus overlay protein binding assay (2-D VOPBA). The expression of crustinPm1 was observed in semigranular and granular hemocytes whereas very low or no expression occurred in hyaline hemocytes. CrustinPm1 appears to either be directly involved in cellular binding or mediating virus internalization into permissive hemocytes.
[Mh] Termos MeSH primário: Hemócitos/metabolismo
Hemócitos/virologia
Proteínas de Membrana/metabolismo
Penaeidae/virologia
Roniviridae/metabolismo
[Mh] Termos MeSH secundário: Animais
Peptídeos Catiônicos Antimicrobianos/metabolismo
Biotinilação
Western Blotting
Eletroforese em Gel Bidimensional
Imunofluorescência
Processamento de Imagem Assistida por Computador
Inibidores de Serino Proteinase/metabolismo
Espectrometria de Massas em Tandem
Ligação Viral
alfa-Macroglobulinas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antimicrobial Cationic Peptides); 0 (Membrane Proteins); 0 (Serine Proteinase Inhibitors); 0 (alpha-Macroglobulins); 0 (crustinPm1 protein, Penaeus monodon)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:141203
[Lr] Data última revisão:
141203
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140831
[St] Status:MEDLINE



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