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  1 / 171 MEDLINE  
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[PMID]:28475029
[Au] Autor:Christiansen DH; McBeath AJA; Aamelfot M; Matejusova I; Fourrier M; White P; Petersen PE; Falk K
[Ad] Endereço:1​Faroese Food and Veterinary Authority, National Reference Laboratory for Fish Diseases, Tórshavn, Faroe Islands.
[Ti] Título:First field evidence of the evolution from a non-virulent HPR0 to a virulent HPR-deleted infectious salmon anaemia virus.
[So] Source:J Gen Virol;98(4):595-606, 2017 Apr.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The putatively non-virulent subtype of infectious salmon anaemia virus (ISAV), ISAV-HPR0, is proposed to act as a progenitor and reservoir for all virulent ISAVs and thus represent a potential risk factor for the emergence of infectious salmon anaemia (ISA) disease. Here, we provide the first evidence of genetic and functional evolution from an ISAV-HPR0 variant (FO/07/12) to a low-virulent ISAV virus (FO/121/14) in a Faroese Atlantic salmon marine farm. The FO/121/14 virus infection was not associated with specific clinical signs of ISA and was confined to a single net-pen, while various ISAV-HPR0 subtypes were found circulating in most epidemiologically linked marine and freshwater farms. Sequence analysis of all eight segments revealed that the FO/121/14 virus was identical, apart from a substitution in the fusion (F) gene (Q266L) and a deletion in the haemagglutinin-esterase (HE) gene, to the FO/07/12 variant from a freshwater farm, which supplied smolts exclusively to the FO/121/14-positive net-pen. An immersion challenge with the FO/121/14 virus induced a systemic infection in Atlantic salmon associated with a low mortality and mild clinical signs confirming its low pathogenicity. Our results demonstrate that mutations in the F protein and deletions in the highly polymorphic region (HPR) of the HE protein represent a minimum requirement for ISAV to gain virulence and to switch cell tropism from a localized epithelial infection to a systemic endotheliotropic infection. This documents that ISAV-HPR0 represents a reservoir and risk factor for the emergence of ISA disease.
[Mh] Termos MeSH primário: Evolução Molecular
Doenças dos Peixes/virologia
Isavirus/genética
Infecções por Orthomyxoviridae/veterinária
[Mh] Termos MeSH secundário: Animais
Isavirus/classificação
Isavirus/isolamento & purificação
Isavirus/patogenicidade
Mutação
Infecções por Orthomyxoviridae/virologia
Filogenia
Salmo salar
Proteínas Virais/genética
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170607
[Lr] Data última revisão:
170607
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170506
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000741


  2 / 171 MEDLINE  
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[PMID]:28202040
[Au] Autor:Zhang W; Cai C; Lin L; Tao YJ; Jin M
[Ad] Endereço:State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
[Ti] Título:Subcellular localization and interactions of Infectious Salmon Anemia Virus (ISAV) M1 and NEP as well as host Hsc70.
[So] Source:Virol J;14(1):30, 2017 Feb 15.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Infectious salmon anemia virus (ISAV) is an important fish pathogen that causes high mortality in farmed Atlantic salmon. The ISAV genome consists of eight single-stranded, negative-sense RNA segments. The six largest segments contain one open reading frame (ORF) each, and encode three polymerase proteins, nucleoprotein, fusion protein, and hemagglutinin esterase protein. The two smallest segments contain more than one ORF each. The segment 7 encodes non-structural protein 1 (NS1) and nuclear export protein (NEP), while segment 8 encodes matrix protein 1 and 2 (M1 and M2). NS1 and M2 have been well known as antagonist of type I interferon. However, little is known about the characterization of M1 or NEP. In addition, heat shock cognate 70 (Hsc70) has been reported to interact with M1 and NEP of influenza viruses for the export of viral ribonucleoprotein (vRNP) via vRNP-M1-NEP complex, the goal of this study therefore was to characterize the subcellular localization and interactions of ISAV M1 and NEP as well as cellular Hsc70. RESULTS: When M1, NEP, and Hsc70 were individually expressed in the stripped snakehead (SSN-1) cells, we found that M1 protein was localized in both cytosol and nucleus of the cells, NEP was localized only in the cytosol and accumulated adjacent to the nucleus, while Hsc70 was localized throughout the cytosol, but not in the nucleus. However, when two of them were co-expressed, we found that both M1 and Hsc70 were co-localized with NEP in the cytosol and accumulated adjacent to the nucleus, while M1 and Hsc70 were still localized as they were expressed individually. Furthermore, pull-down assay was performed and showed that NEP could interact with both M1 and Hsc70, and M1-Hsc70 interaction was also observed although the interaction was weaker than that of NEP-Hsc70. CONCLUSION: Our study characterized the subcellular localization and interactions of three proteins including M1 and NEP of ISAV, and Hsc70. These data will help towards a better understanding of the life cycle of ISAV, especially the process of vRNP export.
[Mh] Termos MeSH primário: Proteínas de Choque Térmico HSC70/metabolismo
Interações Hospedeiro-Patógeno
Isavirus/fisiologia
Mapeamento de Interação de Proteínas
Proteínas Virais/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Ligação Proteica
Salmo salar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (HSC70 Heat-Shock Proteins); 0 (Viral Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170823
[Lr] Data última revisão:
170823
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170217
[St] Status:MEDLINE
[do] DOI:10.1186/s12985-017-0702-z


  3 / 171 MEDLINE  
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[PMID]:28063951
[Au] Autor:Dettleff P; Moen T; Santi N; Martinez V
[Ad] Endereço:FAVET-INBIOGEN, Faculty of Veterinary Sciences, University of Chile, Avda. Santa Rosa 11735, Santiago, Chile. Electronic address: satryl@veterinaria.uchile.cl.
[Ti] Título:Transcriptomic analysis of spleen infected with infectious salmon anemia virus reveals distinct pattern of viral replication on resistant and susceptible Atlantic salmon (Salmo salar).
[So] Source:Fish Shellfish Immunol;61:187-193, 2017 Feb.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The infectious salmon anemia virus (ISAv) produces a systemic infection in salmonids, causing large losses in salmon production. However, little is known regarding the mechanisms exerting disease resistance. In this paper, we perform an RNA-seq analysis in Atlantic salmon challenged with ISAv (using individuals coming from families that were highly susceptible or highly resistant to ISAv infection). We evaluated the differential expression of both host and ISAv genes in a target organ for the virus, i.e. the spleen. The results showed differential expression of host genes related to response to stress, immune response and protein folding (genes such as; atf3, mhc, mx1-3, cd276, cd2, cocs1, c7, il10, il10rb, il13ra2, ubl-1, ifng, ifngr1, hivep2, sigle14 and sigle5). An increased protein processing activity was found in susceptible fish, which generates a subsequent unfolded protein response. We observed extreme differences in the expression of viral segments between susceptible and resistant groups, demonstrating the capacity of resistant fish to overcome the virus replication, generating a very low viral load. This phenomenon and survival of this higher resistant fish seem to be related to differences in immune and translational process, as well as to the increase of HIV-EP2 (hivep2) transcript in resistant fish, although the causal mechanism is yet to be discovered. This study provides valuable information about disease resistance mechanisms in Atlantic salmon from a host-pathogen interaction point of view.
[Mh] Termos MeSH primário: Doenças dos Peixes/genética
Proteínas de Peixes/genética
Infecções por Orthomyxoviridae/veterinária
Salmo salar
Transcriptoma
[Mh] Termos MeSH secundário: Animais
Resistência à Doença
Doenças dos Peixes/imunologia
Doenças dos Peixes/virologia
Proteínas de Peixes/metabolismo
Perfilação da Expressão Gênica/veterinária
Isavirus/fisiologia
Infecções por Orthomyxoviridae/genética
Infecções por Orthomyxoviridae/imunologia
Infecções por Orthomyxoviridae/virologia
Baço/imunologia
Baço/metabolismo
Baço/virologia
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170109
[St] Status:MEDLINE


  4 / 171 MEDLINE  
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[PMID]:27840076
[Au] Autor:Fourrier MC; Collet B
[Ad] Endereço:Marine Scotland Science, Marine Laboratory, AB11 9DB, Aberdeen, United Kingdom. Electronic address: Mickael.Fourrier@gov.scot.
[Ti] Título:Production of infectious salmon anaemia virus (ISAV) ribonucleoprotein complexes using a mammalian cell based minigenome system.
[So] Source:J Virol Methods;239:75-82, 2017 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Developments in recombinant virus techniques have been crucial to understand the mechanisms of virulence acquisition and study the replication of many different negatively stranded RNA viruses. However, such technology has been lacking for infectious salmon anaemia virus (ISAV) until recently. This was due in part to the lack of a Polymerase I promoter in Atlantic salmon to drive the production of recombinant vRNA. Therefore, the present study investigated a different alternative to produce ISAV recombinant vRNA, based on Mouse Pol I promoter/terminator sequences and expression in baby hamster kidney (BHK-21) cells. As a first step, a pathogenic ISAV was demonstrated to replicate and produce viable virions in BHK-21 cells. This indicated that the virus could use the mammalian cellular and nuclear machinery to produce vRNA segments and viral proteins, albeit in a limited capacity. Co-transfection of vRNA expressing plasmids with cytomegalovirus (CMV) promoter constructs coding for the three viral polymerase and nucleoprotein led to the generation of functional ribonucleoproteins (RNPs) which expressed either, green fluorescence protein (GFP) or firefly luciferase (FF). Further experiments demonstrated that a 21h incubation at 37°C was optimal for RNPs production. Inhibition by ribavirin confirmed that FF expression was linked to specific RNPs polymerase transcription. The present minigenome system provides a novel and alternative approach to investigate various aspects of ISAV replication and potentially those of other negatively stranded RNA viruses. Expression of RNPs in mammalian cells could also provide a method for the rapid screening of anti-viral compounds targeting ISAV replication.
[Mh] Termos MeSH primário: Isavirus/genética
Isavirus/fisiologia
Ribonucleoproteínas/biossíntese
Replicação Viral
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
RNA Polimerases Dirigidas por DNA/metabolismo
Proteínas de Fluorescência Verde/química
Isavirus/isolamento & purificação
Luciferases/química
RNA
RNA Viral/genética
Proteínas Recombinantes de Fusão/biossíntese
Ribavirina/farmacologia
Ribonucleoproteínas/química
Ribonucleoproteínas/genética
Ribonucleoproteínas/isolamento & purificação
Salmo salar/virologia
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral); 0 (RNA, recombinant); 0 (Recombinant Fusion Proteins); 0 (Ribonucleoproteins); 0 (Viral Proteins); 147336-22-9 (Green Fluorescent Proteins); 49717AWG6K (Ribavirin); 63231-63-0 (RNA); EC 1.13.12.- (Luciferases); EC 2.7.7.6 (DNA-Directed RNA Polymerases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161115
[St] Status:MEDLINE


  5 / 171 MEDLINE  
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[PMID]:27434377
[Au] Autor:Hoare R; Thompson KD; Herath T; Collet B; Bron JE; Adams A
[Ad] Endereço:Institute of Aquaculture, School of Natural Sciences, University of Stirling, Stirling, FK9 4LA, United Kingdom.
[Ti] Título:Development, Characterisation and Application of Monoclonal Antibodies for the Detection and Quantification of Infectious Salmon Anaemia Virus in Plasma Samples Using Luminex Bead Array Technology.
[So] Source:PLoS One;11(7):e0159155, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Infectious salmon anaemia virus (ISAV) is an orthomyxovirus that has had a significant economic impact on Atlantic salmon farming in Europe, North America and Chile. Monoclonal antibodies (mAbs) were developed against Segment 3 (encoding the viral nucleoprotein, NP) of the virus. Six of the mAbs were shown to be specific to ISAV and recognised all isolates from Scotland, Norway and Canada. They reacted with ISAV in enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody technique (IFAT) and western blotting. They were also used to develop a novel detection method based on Luminex (Bio-Plex) bead-based flow cytometric technology for the detection of ISAV in the plasma of Atlantic salmon (Salmo salar L.) smolts experimentally infected with ISAV. Fish were challenged by intraperitoneal (i.p.) injection of virus at 50% Tissue Culture Infective Dose (TCID50) = 2.8 x106 per animal. Virus present in plasma of infected fish, collected at 0, 4, 8, 12, 16, 21 and 28 days post infection using a non-lethal sampling method (n = 12 at each time point), was quantified using the optimised Bio-Plex assay. The results obtained with this assay were compared with absolute quantification of the virus by RT-qPCR using SYBR Green I and TaqMan chemistries. The Bio-Plex assay developed using the NP mAbs appears to be a rapid, sensitive method for detecting and quantifying ISAV in small volumes of fish plasma and has the potential to be multiplexed for the detection of other fish pathogens (e.g. during co-infections). To our knowledge this is the first report of the use of Luminex (Bio-Plex) technology for the detection of a fish pathogen.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/sangue
Isavirus/isolamento & purificação
Infecções por Orthomyxoviridae/sangue
Infecções por Orthomyxoviridae/virologia
[Mh] Termos MeSH secundário: Animais
Canadá
Chile
Ensaio de Imunoadsorção Enzimática
Europa (Continente)
Doenças dos Peixes/virologia
Isavirus/patogenicidade
América do Norte
Noruega
Infecções por Orthomyxoviridae/veterinária
Salmo salar/sangue
Salmo salar/virologia
Escócia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160720
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0159155


  6 / 171 MEDLINE  
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[PMID]:27108379
[Au] Autor:Robertsen B; Chang CJ; Bratland L
[Ad] Endereço:Norwegian College of Fishery Science, UiT The Arctic University of Norway, 9037 Tromsø, Norway. Electronic address: borre.robertsen@uit.no.
[Ti] Título:IFN-adjuvanted DNA vaccine against infectious salmon anemia virus: Antibody kinetics and longevity of IFN expression.
[So] Source:Fish Shellfish Immunol;54:328-32, 2016 Jul.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Plasmids expressing interferon (IFN) have recently been shown to function as adjuvants in Atlantic salmon when co-injected with a DNA vaccine encoding hemagglutinin-esterase (HE) from infectious salmon anemia virus (ISAV). In this work we have compared the antibody kinetics and the systemic Mx/ISG15 response of fish vaccinated with HE-plasmid using either IFNa plasmid (pIFNa) or pIFNc as adjuvants over a longer time period, i.e. 22 weeks post vaccination (pv). The results showed that the antibody response against ISAV with pIFNa as adjuvant arose earlier (7 weeks pv) than with pIFNc as adjuvant (10 weeks pv), peaked at week 10 and declined at week 22. The antibody response with pIFNc as adjuvant peaked at 16 weeks and kept at this level 22 weeks pv. Fish injected with pIFNc alone expressed high levels of Mx and ISG15 in liver throughout the 22 week period. In contrast, fish injected with pIFNc together with HE-plasmid expressed high levels of Mx and ISG15 in liver for the first 10 weeks, but at week 16 this response was absent in two of three fish at week 16 and was absent in all tested fish at week 22 pv. This suggests that cells expressing HE and IFNc are intact at week 10 pv, but are eliminated by adaptive immune responses after week 10 due to recognition of HE. The longevity of the Mx/ISG15 response in pIFNc treated fish is likely due to the fact that IFNc is a self-antigen of salmon and is not attacked by the adaptive immune system.
[Mh] Termos MeSH primário: Doenças dos Peixes/prevenção & controle
Interferons/genética
Isavirus/imunologia
Infecções por Orthomyxoviridae/veterinária
Salmo salar
Vacinas Virais/imunologia
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos/metabolismo
Animais
Anticorpos Antivirais/metabolismo
Doenças dos Peixes/virologia
Hemaglutininas Virais/genética
Hemaglutininas Virais/metabolismo
Interferons/metabolismo
Cinética
Infecções por Orthomyxoviridae/prevenção & controle
Infecções por Orthomyxoviridae/virologia
Vacinas de DNA/imunologia
Proteínas Virais de Fusão/genética
Proteínas Virais de Fusão/metabolismo
Proteínas Virais/genética
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Antibodies, Viral); 0 (Hemagglutinins, Viral); 0 (Vaccines, DNA); 0 (Viral Fusion Proteins); 0 (Viral Proteins); 0 (Viral Vaccines); 0 (hemagglutinin esterase); 9008-11-1 (Interferons)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170209
[Lr] Data última revisão:
170209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160425
[St] Status:MEDLINE


  7 / 171 MEDLINE  
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[PMID]:27012395
[Au] Autor:Li C; Greiner-Tollersrud L; Robertsen B
[Ad] Endereço:Norwegian College of Fishery Science, University of Tromsø, The Arctic University of Norway, N-9037, Tromsø, Norway.
[Ti] Título:Infectious salmon anemia virus segment 7 ORF1 and segment 8 ORF2 proteins inhibit IRF mediated activation of the Atlantic salmon IFNa1 promoter.
[So] Source:Fish Shellfish Immunol;52:258-62, 2016 May.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Infectious salmon anemia virus (ISAV) is an orthomyxovirus, which may cause multisystemic disease and high mortality of Atlantic salmon (Salmo salar L). This suggests that ISAV encodes proteins that antagonize the type I interferon (IFN-I) system, which is of crucial importance in innate antiviral immunity. To find out how ISAV might inhibit IFN-I synthesis, we have here studied whether the two ISAV proteins s7ORF1 and s8ORF2 might interfere with activation of the IFNa1 promoter mediated by overexpression of interferon regulatory factors (IRFs) or by the IFN promoter activation protein IPS-1. The IRF tested were IRF1, IRF3, IRF7A and IRF7B. Promoter activation was measured using a luciferase reporter assay where Atlantic salmon TO cells were co-transfected with the IFNa1 promoter reporter plasmid together with an IRF plasmid and the s7ORF1 or the s8ORF2 construct or a control plasmid. The results showed that s7ORF1 significantly inhibited IRF3 and IRF7B induced IFN promoter activity, while s8ORF2 significantly inhibited IRF1 and IRF3 induced promoter activity. Neither s7ORF1 nor s8ORF2 inhibited IPS-1 mediated promoter activation. Immunoprecipitation data suggest that both s7ORF1 and s8ORF2 can bind to all four IRFs. Taken together, this study thus shows that the ISAV proteins s7ORF1 and s8ORF2 antagonizes IFN-I transcription activation mediated by the IRFs. As such this work provides further insight into the pathogenic properties of ISAV.
[Mh] Termos MeSH primário: Doenças dos Peixes/imunologia
Interferon-alfa/genética
Isavirus/fisiologia
Infecções por Orthomyxoviridae/veterinária
Salmo salar
Transcrição Genética
[Mh] Termos MeSH secundário: Animais
Doenças dos Peixes/microbiologia
Proteínas de Peixes/genética
Proteínas de Peixes/metabolismo
Genes Virais
Fatores Reguladores de Interferon/genética
Fatores Reguladores de Interferon/metabolismo
Interferon-alfa/metabolismo
Isavirus/genética
Fases de Leitura Aberta
Infecções por Orthomyxoviridae/imunologia
Infecções por Orthomyxoviridae/microbiologia
Regiões Promotoras Genéticas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fish Proteins); 0 (Interferon Regulatory Factors); 0 (Interferon-alpha)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161217
[Lr] Data última revisão:
161217
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160326
[St] Status:MEDLINE


  8 / 171 MEDLINE  
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[PMID]:26999815
[Au] Autor:Aamelfot M; Christiansen DH; Dale OB; McBeath A; Benestad SL; Falk K
[Ad] Endereço:Norwegian Veterinary Institute, Oslo, Norway.
[Ti] Título:Localised Infection of Atlantic Salmon Epithelial Cells by HPR0 Infectious Salmon Anaemia Virus.
[So] Source:PLoS One;11(3):e0151723, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Infectious salmon anaemia (ISA) is an important, systemic viral disease of farmed Atlantic salmon, Salmo salar L. Endothelial cells are the main target cells for highly virulent HPR-deleted ISA virus (ISAV) types. Here we examine the pathogenesis of non-virulent ISAV HPR0 infections, presenting evidence of an epithelial tropism for this virus type, including actual infection and replication in the epithelial cells. Whereas all HPR0 RT-qPCR positive gills prepared for cryosection tested positive by immunohistochemistry (IHC) and immunofluorescent labelling, only 21% of HPR0 RT-qPCR positive formalin-fixed paraffin-embedded gills were IHC positive, suggesting different methodological sensitivities. Only specific epithelial cell staining was observed and no staining was observed in endothelial cells of positive gills. Furthermore, using an ISAV segment 7 RT-PCR assay, we demonstrated splicing of HPR0, suggesting initial activation of the replication machinery in the epithelial gill cells. Immunological responses were investigated by the expression of interferon-related genes (e.g. Mx and γIP) and by ELISA for presence of anti-ISAV antibodies on samples taken sequentially over several months during an episode of transient HPR0 infection. All fish revealed a variable, but increased expression of the immunological markers in comparison to normal healthy fish. Taken together, we conclude that HPR0 causes a localized epithelial infection of Atlantic salmon.
[Mh] Termos MeSH primário: Células Epiteliais/virologia
Doenças dos Peixes/virologia
Isavirus/fisiologia
Infecções por Orthomyxoviridae/virologia
Salmo salar/virologia
[Mh] Termos MeSH secundário: Nadadeiras de Animais/virologia
Animais
Autopsia
Biomarcadores/metabolismo
Ensaio de Imunoadsorção Enzimática
Células Epiteliais/patologia
Doenças dos Peixes/patologia
Imunofluorescência
Brânquias/virologia
Imuno-Histoquímica
Infecções por Orthomyxoviridae/patologia
Reação em Cadeia da Polimerase em Tempo Real
Salmo salar/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:160324
[Lr] Data última revisão:
160324
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160322
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0151723


  9 / 171 MEDLINE  
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[PMID]:26994669
[Au] Autor:Caruffo M; Maturana C; Kambalapally S; Larenas J; Tobar JA
[Ad] Endereço:Virbac-Centrovet, Av. Salomón Sack 255, Cerrillos, Santiago, Chile; Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Av. Santa Rosa, 11735, La Pintana, Santiago, Chile.
[Ti] Título:Protective oral vaccination against infectious salmon anaemia virus in Salmo salar.
[So] Source:Fish Shellfish Immunol;54:54-9, 2016 Jul.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Infectious salmon anemia (ISA) is a systemic disease caused by an orthomyxovirus, which has a significant economic impact on the production of Atlantic salmon (Salmo salar). Currently, there are several commercial ISA vaccines available, however, those products are applied through injection, causing stress in the fish and leaving them susceptible to infectious diseases due to the injection process and associated handling. In this study, we evaluated an oral vaccine against ISA containing a recombinant viral hemagglutinin-esterase and a fusion protein as antigens. Our findings indicated that oral vaccination is able to protect Atlantic salmon against challenge with a high-virulence Chilean isolate. The oral vaccination was also correlated with the induction of IgM-specific antibodies. On the other hand, the vaccine was unable to modulate expression of the antiviral related gene Mx, showing the importance of the humoral response to the disease survival. This study provides new insights into fish protection and immune response induced by an oral vaccine against ISA, but also promises future development of preventive solutions or validation of the current existing therapies.
[Mh] Termos MeSH primário: Doenças dos Peixes/prevenção & controle
Isavirus/imunologia
Infecções por Orthomyxoviridae/veterinária
Salmo salar
Vacinação/veterinária
[Mh] Termos MeSH secundário: Administração Oral
Animais
Chile
Doenças dos Peixes/virologia
Infecções por Orthomyxoviridae/prevenção & controle
Infecções por Orthomyxoviridae/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170217
[Lr] Data última revisão:
170217
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160321
[St] Status:MEDLINE


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[PMID]:26939752
[Au] Autor:Boltaña S; Valenzuela-Miranda D; Aguilar A; Mackenzie S; Gallardo-Escárate C
[Ad] Endereço:Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, Concepción, Chile.
[Ti] Título:Long noncoding RNAs (lncRNAs) dynamics evidence immunomodulation during ISAV-Infected Atlantic salmon (Salmo salar).
[So] Source:Sci Rep;6:22698, 2016 Mar 04.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Despite evidence for participation in the host response to infection, the roles of many long non-coding RNAs (lncRNAs) remain unknown. Therefore, the aims of this study were to identify lncRNAs in Atlantic salmon (Salmo salar) and evaluate their transcriptomic regulation during ISA virus (ISAV) infection, an Orthomyxoviridae virus associated with high mortalities in salmonid aquaculture. Using next-generation sequencing, whole-transcriptome analysis of the Salmo salar response to ISAV infection was performed, identifying 5,636 putative lncRNAs with a mean length of 695 base pairs. The transcriptional modulation evidenced a similar number of differentially expressed lncRNAs in the gills (3,294), head-kidney (3,275), and liver (3,325) over the course of the infection. Moreover, analysis of a subset of these lncRNAs showed the following: (i) Most were similarly regulated in response to ISA virus infection; (ii) The transcript subsets were uniquely modulated in each tissue (gills, liver, and head-kidney); and (iii) A subset of lncRNAs were upregulated for each tissue and time analysed, indicating potential markers for ISAV infection. These findings represent the first discovery of widespread differential expression of lncRNAs in response to virus infection in non-model species, suggesting that lncRNAs could be involved in regulating the host response during ISAV infection.
[Mh] Termos MeSH primário: Doenças dos Peixes/patologia
Imunomodulação
Isavirus/imunologia
Infecções por Orthomyxoviridae/veterinária
RNA Longo não Codificante/análise
Salmo salar
[Mh] Termos MeSH secundário: Animais
Doenças dos Peixes/virologia
Perfilação da Expressão Gênica
Brânquias/patologia
Sequenciamento de Nucleotídeos em Larga Escala
Isavirus/patogenicidade
Rim/patologia
Fígado/patologia
Infecções por Orthomyxoviridae/patologia
RNA Longo não Codificante/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Long Noncoding)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170105
[Lr] Data última revisão:
170105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160305
[St] Status:MEDLINE
[do] DOI:10.1038/srep22698



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