Base de dados : MEDLINE
Pesquisa : B04.820.562 [Categoria DeCS]
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  1 / 73 MEDLINE  
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[PMID]:28759053
[Au] Autor:Legoff J; Resche-Rigon M; Bouquet J; Robin M; Naccache SN; Mercier-Delarue S; Federman S; Samayoa E; Rousseau C; Piron P; Kapel N; Simon F; Socié G; Chiu CY
[Ad] Endereço:University of Paris Diderot, Sorbonne Paris Cité, Inserm U941, Microbiology Laboratory, Hôpital Saint-Louis, APHP, Paris, France.
[Ti] Título:The eukaryotic gut virome in hematopoietic stem cell transplantation: new clues in enteric graft-versus-host disease.
[So] Source:Nat Med;23(9):1080-1085, 2017 Sep.
[Is] ISSN:1546-170X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Much attention has been focused on the role of the bacterial microbiome in human health, but the virome is understudied. Although previously investigated in individuals with inflammatory bowel diseases or solid-organ transplants, virome dynamics in allogeneic hematopoietic stem cell transplantation (HSCT) and enteric graft-versus-host disease (GVHD) remain unexplored. Here we characterize the longitudinal gut virome in 44 recipients of HSCT using metagenomics. A viral 'bloom' was identified, and significant increases were demonstrated in the overall proportion of vertebrate viral sequences following transplantation (P = 0.02). Increases in both the rates of detection (P < 0.0001) and number of sequences (P = 0.047) of persistent DNA viruses (anelloviruses, herpesviruses, papillomaviruses and polyomaviruses) over time were observed in individuals with enteric GVHD relative to those without, a finding accompanied by a reduced phage richness (P = 0.01). Picobirnaviruses were detected in 18 individuals (40.9%), more frequently before or within a week after transplant than at later time points (P = 0.008). In a time-dependent Cox proportional-hazards model, picobirnaviruses were predictive of the occurrence of severe enteric GVHD (hazard ratio, 2.66; 95% confidence interval (CI) = 1.46-4.86; P = 0.001), and correlated with higher fecal levels of two GVHD severity markers, calprotectin and α1-antitrypsin. These results reveal a progressive expansion of vertebrate viral infections over time following HSCT, and they suggest an unexpected association of picobirnaviruses with early post-transplant GVHD.
[Mh] Termos MeSH primário: DNA Viral/análise
Microbioma Gastrointestinal/imunologia
Doença Enxerto-Hospedeiro/imunologia
Transplante de Células-Tronco Hematopoéticas
Enteropatias/imunologia
Intestinos/virologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Anelloviridae/genética
Anelloviridae/imunologia
Fezes/química
Feminino
Microbioma Gastrointestinal/genética
Herpesviridae/genética
Herpesviridae/imunologia
Seres Humanos
Complexo Antígeno L1 Leucocitário/metabolismo
Masculino
Metagenômica
Meia-Idade
Papillomaviridae/genética
Papillomaviridae/imunologia
Picobirnavirus/genética
Picobirnavirus/imunologia
Polyomaviridae/genética
Polyomaviridae/imunologia
Modelos de Riscos Proporcionais
Fatores de Risco
Índice de Gravidade de Doença
Transplante Homólogo
Adulto Jovem
alfa 1-Antitripsina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Leukocyte L1 Antigen Complex); 0 (alpha 1-Antitrypsin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE
[do] DOI:10.1038/nm.4380


  2 / 73 MEDLINE  
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[PMID]:28032197
[Au] Autor:Wilburn L; Yodmeeklin A; Kochjan P; Saikruang W; Kumthip K; Khamrin P; Maneekarn N
[Ad] Endereço:Faculty of Life Sciences, University of Manchester, Manchester, UK.
[Ti] Título:Molecular detection and characterization of picobirnaviruses in piglets with diarrhea in Thailand.
[So] Source:Arch Virol;162(4):1061-1066, 2017 Apr.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Picobirnavirus (PBV) is a small, bi-segmented, double-stranded RNA virus. Taxonomically, the genus Picobirnavirus belongs to the Picobirnaviridae family. PBV infects a wide range of hosts and causes opportunistic infections, but its role in diarrheal disease remains unclear. To determine the prevalence and genetic diversity of porcine PBVs in Northern Thailand, 380 fecal samples collected from diarrheic and non-diarrheic piglets, raised in 22 pig farms, were tested for the presence of PBV. Reverse-transcription PCR (RT-PCR) was performed using primer sets specific to the RNA-dependent RNA polymerase (RdRp) gene. PBV was detected in 86 of 265 (32.5%) diarrheic piglets and in 26 of 115 (22.6%) non-diarrheic piglets. All the PBV strains detected in this study belonged to genogroup I and a high proportion of PBV-positive piglets were co-infected with group A rotavirus (RVA) and bocavirus (BoV). Phylogenetic analysis of representative genogroup I strains revealed remarkably high similarity between strains; these formed a monophyletic cluster with 97-100% sequence identity in the RdRp gene. The strains were also closely related to genogroup I PBV Chinese porcine strain. The findings indicate that PBV infection is common in piglets with and without diarrhea in Northern Thailand.
[Mh] Termos MeSH primário: Diarreia/veterinária
Picobirnavirus/genética
Infecções por Vírus de RNA/veterinária
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Animais
Diarreia/virologia
Fezes/virologia
Filogenia
Picobirnavirus/classificação
Picobirnavirus/isolamento & purificação
Picobirnavirus/fisiologia
Infecções por Vírus de RNA/virologia
Suínos
Tailândia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170315
[Lr] Data última revisão:
170315
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161230
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-016-3190-3


  3 / 73 MEDLINE  
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[PMID]:27987110
[Au] Autor:Kylla H; Dutta TK; Roychoudhury P; Malik YS; Mandakini R; Subudhi PK
[Ad] Endereço:Department of Veterinary Microbiology, Central Agricultural University, Selesih, Aizawl, Mizoram, 796014, India.
[Ti] Título:Prevalence and molecular characterization of porcine Picobirnavirus in piglets of North East Region of India.
[So] Source:Trop Anim Health Prod;49(2):417-422, 2017 Feb.
[Is] ISSN:1573-7438
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Picobirnaviruses (PBVs) have been recognized as one of the important causal viral agents of gastroenteritis in several animal species especially in young immunocompromised hosts. In this study, we report the prevalence and molecular epidemiology of porcine PBVs from North Eastern Hilly region of India. A total of 457 fecal samples from piglets were collected from local (n = 130) and cross (n = 327) breed piglets in different seasons for 2 years. All the samples were subjected to RNA-PAGE and RT-PCR analysis for detection of PBVs. A total of 4.59 and 11.15% samples were recorded as positive for PBVs by RNA-PAGE and RT-PCR, respectively. Rate of detection was higher from diarrhoeic animals (13.56%) compared to non-diarrhoeic (4.23%) animals. Higher prevalence rate was observed from unorganized farms (14.22%) compared to organized farms (8.0%) with slightly higher detection from cross breed (11.62%) compared to local breed (10.0%). Maximum cases of piglet diarrhea associated with PBVs were detected during summer (16.4%) and winter (14.39%) seasons compared to autumn (4.80%) and spring (6.45%). All the samples were positive for PBV genogroup I only. Based upon the sequence analysis, the isolates were unique and placed in separate clad and were not closely associated with any other Indian isolates of PBVs so far. Two isolates were closely related with one Chinese isolate recovered from sewage water. This is the first systematic study of prevalence of PBVs associated with piglet diarrhea.
[Mh] Termos MeSH primário: Diarreia/virologia
Fezes/virologia
Picobirnavirus/genética
Infecções por Vírus de RNA/virologia
Doenças dos Suínos/epidemiologia
Suínos/virologia
[Mh] Termos MeSH secundário: Animais
Clonagem Molecular
Gastroenterite/virologia
Índia/epidemiologia
Epidemiologia Molecular
Filogenia
Picobirnavirus/isolamento & purificação
Prevalência
Infecções por Vírus de RNA/epidemiologia
RNA de Cadeia Dupla/análise
Estações do Ano
Esgotos
Doenças dos Suínos/virologia
Microbiologia da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Double-Stranded); 0 (Sewage)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161218
[St] Status:MEDLINE
[do] DOI:10.1007/s11250-016-1210-7


  4 / 73 MEDLINE  
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[PMID]:27438074
[Au] Autor:Conceição-Neto N; Mesquita JR; Zeller M; Yinda CK; Álvares F; Roque S; Petrucci-Fonseca F; Godinho R; Heylen E; Van Ranst M; Matthijnssens J
[Ad] Endereço:Laboratory of Viral Metagenomics, Department of Microbiology and Immunology, Rega Institute for Medical Research, KU Leuven, University of Leuven, Leuven, Belgium.
[Ti] Título:Reassortment among picobirnaviruses found in wolves.
[So] Source:Arch Virol;161(10):2859-62, 2016 Oct.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:We conducted a viral metagenomics study in diarrheic free-ranging wolves in Portugal, revealing for the first time the presence of reassortant picobirnaviruses. These viruses shared identical capsid segments together with diverse RNA-dependent RNA polymerase segments. Even though causality between these picobirnaviruses and diarrhea could not be established, the study nonetheless confirms for the first time that wolves are a potential reservoir for picobirnaviruses, which might play a role as enteric pathogens.
[Mh] Termos MeSH primário: Variação Genética
Picobirnavirus/genética
Infecções por Vírus de RNA/veterinária
Vírus Reordenados/genética
Lobos/virologia
[Mh] Termos MeSH secundário: Animais
Metagenômica
Picobirnavirus/isolamento & purificação
Portugal
Infecções por Vírus de RNA/virologia
Vírus Reordenados/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170123
[Lr] Data última revisão:
170123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160721
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-016-2987-4


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[PMID]:27012914
[Au] Autor:Lojkic I; Bidin M; Prpic J; Simic I; Kresic N; Bedekovic T
[Ad] Endereço:Department of Virology, Croatian Veterinary Institute, Zagreb, Croatia. Electronic address: ilojkic@veinst.hr.
[Ti] Título:Faecal virome of red foxes from peri-urban areas.
[So] Source:Comp Immunol Microbiol Infect Dis;45:10-5, 2016 Apr.
[Is] ISSN:1878-1667
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Red foxes (Vulpes vulpes) are the most abundant carnivore species in the Northern Hemisphere. Since their populations are well established in peri-urban and urban areas, they represent a potential reservoir of viruses that transmit from wildlife to humans or domestic animals. In this study, we evaluated the faecal virome of juvenile and adult foxes from peri-urban areas in central Croatia. The dominating mammalian viruses were fox picobirnavirus and parvovirus. The highest number of viral reads (N=1412) was attributed to a new fox circovirus and complete viral genome was de novo assembled from the high-throughput sequencing data. Fox circovirus is highly similar to dog circoviruses identified in diseased dogs in USA and Italy, and to a recently discovered circovirus of foxes with neurologic disease from the United Kingdom. Our fox picobirnavirus was more closely related to the porcine and human picobirnaviruses than to known fox picobirnaviruses.
[Mh] Termos MeSH primário: Circovirus/isolamento & purificação
Fezes/virologia
Raposas/virologia
Microbiota
Parvovirus/isolamento & purificação
Picobirnavirus/isolamento & purificação
População Urbana
[Mh] Termos MeSH secundário: Animais
Animais Domésticos
Circovirus/classificação
Circovirus/genética
Croácia
Reservatórios de Doenças/veterinária
Reservatórios de Doenças/virologia
Cães
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Metagenoma
Parvovirus/classificação
Parvovirus/genética
Filogenia
Picobirnavirus/classificação
Picobirnavirus/genética
Análise de Sequência de DNA
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160326
[St] Status:MEDLINE


  6 / 73 MEDLINE  
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Roehe, Paulo Michel
PubMed Central Texto completo
Texto completo
[PMID]:26986573
[Au] Autor:Kluge M; Campos FS; Tavares M; de Amorim DB; Valdez FP; Giongo A; Roehe PM; Franco AC
[Ad] Endereço:Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, UFRGS (Federal University of Rio Grande do Sul), Porto Alegre, Rio Grande do Sul, Brazil.
[Ti] Título:Metagenomic Survey of Viral Diversity Obtained from Feces of Subantarctic and South American Fur Seals.
[So] Source:PLoS One;11(3):e0151921, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Brazilian South coast seasonally hosts numerous marine species, observed particularly during winter months. Some animals, including fur seals, are found dead or debilitated along the shore and may harbor potential pathogens within their microbiota. In the present study, a metagenomic approach was performed to evaluate the viral diversity in feces of fur seals found deceased along the coast of the state of Rio Grande do Sul. The fecal virome of two fur seal species was characterized: the South American fur seal (Arctocephalus australis) and the Subantarctic fur seal (Arctocephalus tropicalis). Fecal samples from 10 specimens (A. australis, n = 5; A. tropicalis, n = 5) were collected and viral particles were purified, extracted and amplified with a random PCR. The products were sequenced through Ion Torrent and Illumina platforms and assembled reads were submitted to BLASTx searches. Both viromes were dominated by bacteriophages and included a number of potentially novel virus genomes. Sequences of picobirnaviruses, picornaviruses and a hepevirus-like were identified in A. australis. A rotavirus related to group C, a novel member of the Sakobuvirus and a sapovirus very similar to California sea lion sapovirus 1 were found in A. tropicalis. Additionally, sequences of members of the Anelloviridae and Parvoviridae families were detected in both fur seal species. This is the first metagenomic study to screen the fecal virome of fur seals, contributing to a better understanding of the complexity of the viral community present in the intestinal microbiota of these animals.
[Mh] Termos MeSH primário: Otárias/virologia
Metagenômica
Vírus/genética
[Mh] Termos MeSH secundário: Anelloviridae/genética
Animais
Bacteriófagos/genética
Sequência de Bases
Fezes/virologia
Genoma Viral/genética
Biblioteca Genômica
Herpesviridae/genética
Dados de Sequência Molecular
Parvoviridae/genética
Filogenia
Picobirnavirus/genética
Picornaviridae/genética
Rotavirus/genética
Sapovirus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1608
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160318
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0151921


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[PMID]:26695771
[Au] Autor:Sun G; Zang Q; Gu Y; Niu G; Ding C; Zhang P
[Ad] Endereço:Xuzhou Central Hospital, 199 South Jiefang Road, Xuzhou, 221001, China.
[Ti] Título:Viral metagenomics analysis of picobirnavirus-positive feces from children with sporadic diarrhea in China.
[So] Source:Arch Virol;161(4):971-5, 2016 Apr.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Picobirnaviruses (PBVs) infect humans and a wide range of animals and may cause diarrhea. The aim of this study was to investigate PBV infection and its association with diarrhea. Here, seven PBV RT-PCR-positive fecal samples from diarrheic children and four fecal samples from healthy children as controls were analyzed by viral metagenomics. The results indicated that all the seven diarrheic fecal samples contain high titers of PBV sequences, while three of the controls were negative, and one had low titers of PBV. Three of the diarrheic fecal samples were also positive for other viruses, including anellovirus, human gyrovirus, human parechovirus, and porcine stool-associated circular virus. PBV sequences from the seven patients were assembled, generating seven large contigs with the complete ORF of RNA-dependent RNA polymerase (RdRp). Phylogenetic analysis of the amino acid sequences of RdRp indicated that the seven PBVs in the present study belonged to three different genogroups. Our data suggest that PBV might have been the cause of diarrhea in these seven children.
[Mh] Termos MeSH primário: Diarreia/virologia
Fezes/virologia
Genoma Viral
Picobirnavirus/isolamento & purificação
Infecções por Vírus de RNA/virologia
[Mh] Termos MeSH secundário: Criança
Pré-Escolar
China/epidemiologia
Diarreia/epidemiologia
Seres Humanos
Metagenômica
Filogenia
Infecções por Vírus de RNA/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1608
[Cu] Atualização por classe:160330
[Lr] Data última revisão:
160330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151224
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-015-2726-2


  8 / 73 MEDLINE  
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Alfieri, Amauri Alcindo
Texto completo
[PMID]:26435337
[Au] Autor:Takiuchi E; Macedo R; Kunz AF; Gallego JC; de Mello JL; Otonel RA; Alfieri AA
[Ad] Endereço:Department of Veterinary Sciences, Federal University of Parana - UFPR, 85950-000, Palotina, PR, Brazil. Electronic address: e.takiuchi@ufpr.br.
[Ti] Título:Electrophoretic RNA genomic profiles of Brazilian Picobirnavirus (PBV) strains and molecular characterization of a PBV isolated from diarrheic calf.
[So] Source:Virus Res;211:58-63, 2016 Jan 04.
[Is] ISSN:1872-7492
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Picobirnavirus (PBV) belongs to the family Picobirnaviridae. PBV are a group of emerging non-enveloped viruses, with a bisegmented double-stranded RNA genome that can infect a wide range of hosts. This study reports the occurrence of PBV in fecal samples from five Brazilian dairy cattle herds. From the 289 stool samples of individual calves analyzed by silver-stained polyacrylamide gel electrophoresis (ss-PAGE) the PBV was detected in 8.3 % (24/289), of which 10.2% (18/176) had diarrheic consistency. Of the 24 positive samples in ss-PAGE, 5 (20.8%) of them showed a small electrophoretic profile and 19 (79.2%) samples had large profile. From the 24 positives samples by ss-PAGE, 15 (62.5%) were successfully amplified (201 bp) using GI specific primers targeting the RdRp gene of PBV. The analysis of nucleotide identity matrix revealed that the bovine PBV strain identified in this study, showed the highest nucleotide identity (81%) with PBV strain detected in turkey (MD-2010/HM803965). This is the first nucleotide sequence of a bovine PBV strain in the American continent and the first detection of small genome profile of PBV-like strains in bovine hosts.
[Mh] Termos MeSH primário: Doenças dos Bovinos/virologia
Diarreia/veterinária
Picobirnavirus/genética
Infecções por Vírus de RNA/veterinária
RNA Viral/genética
[Mh] Termos MeSH secundário: Animais
Brasil
Bovinos
Diarreia/virologia
Eletroforese em Gel de Poliacrilamida
Fezes/virologia
Filogenia
Picobirnavirus/química
Picobirnavirus/classificação
Picobirnavirus/isolamento & purificação
Infecções por Vírus de RNA/virologia
RNA Viral/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151006
[St] Status:MEDLINE


  9 / 73 MEDLINE  
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PubMed Central Texto completo
Texto completo
[PMID]:25546400
[Au] Autor:Zhang S; Bai R; Feng R; Zhang H; Liu L
[Ad] Endereço:College of Life Science, University of Chinese Academy of Sciences, No.19A, Yuquan Road, Shijingshan District, Beijing, 100049, China.
[Ti] Título:Detection and evolutionary analysis of picobirnaviruses in treated wastewater.
[So] Source:Microb Biotechnol;8(3):474-82, 2015 May.
[Is] ISSN:1751-7915
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Wastewater contains numerous viruses. In this study, picobirnaviruses (PBVs) were detected in the stream of a wastewater treatment plant in Changsha, Hunan province, China, and evolutionary analysis of the isolated PBVs was performed. The phylogenetic tree revealed that the PBVs were highly divergent and could be classified into six distinct groups according to their hosts. Among these groups, pairwise comparison of the six groups revealed that the nucleotide distance of group 4 (bootstrap value = 0.92; nucleotide identity = 94%) was the largest. Thus, group 4 might represent a new division of PBVs. Comprehensive analysis of the obtained PBV sequences to investigate their evolutionary history and phylodynamics revealed that group 5 (PBVs from monkey) exhibited maximum polymorphism (K = 30.582, S = 74, η = 98, Pa = 47) and lowest nucleotide substitutions per site per year (6.54E-3 subs per site per year), except group 4. Maximum clade credibility tree indicated that group 5 appeared earlier than the other groups. In conclusion, this study detected PBVs in treated wastewater in China, and identified a new PBV group. Furthermore, among these PBVs, group 5 was found to survive longer and present a balance between PBVs and their monkey host.
[Mh] Termos MeSH primário: Variação Genética
Filogenia
Picobirnavirus/classificação
Picobirnavirus/isolamento & purificação
Águas Residuais/virologia
[Mh] Termos MeSH secundário: China
Análise por Conglomerados
Genótipo
Dados de Sequência Molecular
Picobirnavirus/genética
RNA Viral/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Viral); 0 (Waste Water)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141230
[St] Status:MEDLINE
[do] DOI:10.1111/1751-7915.12239


  10 / 73 MEDLINE  
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[PMID]:25530436
[Au] Autor:Verma H; Mor SK; Erber J; Goyal SM
[Ad] Endereço:Department of Veterinary Population Medicine and Veterinary Diagnostic Laboratory, University of Minnesota, 1333 Gortner Ave, St. Paul, MN 55108, USA.
[Ti] Título:Prevalence and complete genome characterization of turkey picobirnaviruses.
[So] Source:Infect Genet Evol;30:134-9, 2015 Mar.
[Is] ISSN:1567-7257
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The "light turkey syndrome" (LTS), in which birds weigh less than their standard breed character at the marketing time, is believed to be a consequence of viral enteritis at an early age (3-5 weeks) from which the birds never fully recover. In a previously published study, we collected fecal pools from 2, 3, 5 and 8 week old turkey poults (80 pools from LTS farms and 40 from non-LTS farms) and examined them for the presence of astro-, rota-, reo-, and coronaviruses. To determine the presence of additional enteric viruses, we analyzed a fecal pool by Illumina sequencing and found picobirnavirus (PBV). Segments 1 and 2 of this virus shared 45.8%aa and 60.9-64.5%aa identity with genogroup I of human PBV, respectively. Primers based on RNA-dependent RNA polymerase and capsid genes were designed for detection and molecular characterization of PBVs in the 120 fecal pools described above. From LTS farms, 39 of 80 (48.8%) pools were PBV positive while 23 of 40 (57.5%) were positive from non-LTS farms. The phylogenetic analysis of 15 randomly selected strains divided them into four subgroups within genogroup I (subgroups 1A-D). Nine strains were in subgroup IA showing 69.9-76.4%nt identity with human PBV GI strainVS111 from the Netherlands. Strains in subgroup IB (n=2) had 91.4-91.7%nt identity with chicken PBV GI strain AVE 42v1 from Brazil. Two strains in subgroup IC had 72.3-74.2%nt identity with chicken PBV strain AVE 71v3 from Brazil. In subgroup ID, two strains showed 72.4-81.8%nt identity with chicken PBV GI strain AVE 57v2 from Brazil. Subgroup IC and ID were the most divergent. Five of the 15 strains were typed using capsid gene primers. They showed 32.6-33.4%nt and 39.5-41.3%aa identity with VS10 human PBV strain. These results indicate co-circulation of divergent strains of PBVs among Minnesota turkeys.
[Mh] Termos MeSH primário: Proteínas do Capsídeo/genética
Picobirnavirus/genética
Doenças das Aves Domésticas/virologia
Infecções por Vírus de RNA/virologia
Perus/virologia
[Mh] Termos MeSH secundário: Animais
Fezes/virologia
Filogenia
Picobirnavirus/classificação
Doenças das Aves Domésticas/epidemiologia
Infecções por Vírus de RNA/epidemiologia
Infecções por Vírus de RNA/veterinária
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Capsid Proteins)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:150203
[Lr] Data última revisão:
150203
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141223
[St] Status:MEDLINE



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