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[PMID]:28795263
[Au] Autor:Fan L; Li Z; Huang J; Yang Z; Xiao S; Wang X; Dang R; Zhang S
[Ad] Endereço:College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, China.
[Ti] Título:Dynamic distribution and tissue tropism of avian encephalomyelitis virus isolate XY/Q-1410 in experimentally infected Korean quail.
[So] Source:Arch Virol;162(11):3447-3458, 2017 Nov.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Avian encephalomyelitis (AE) is an important infectious poultry disease worldwide that is caused by avian encephalomyelitis virus (AEV). However, to date, the dynamic distribution of AEV in quails has not been well described. Quantitative real-time polymerase chain reaction (qPCR) and immunohistochemistry (IHC) assays were used to investigate the dynamic distribution and tissue tropism of AEV in experimentally infected Korean quail. AEV was detected in the cerebrum, cerebellum, proventriculus, intestine, liver, pancreas, spleen, bursa, lung and kidney as early as 3 days post-infection (dpi). The viral loads in the proventriculus, intestine, spleen and bursa were relatively higher than in other tissues. According to the qPCR results, AEV XY/Q-1410 infection lasted for at least 60 days in infected Korean quail. Immunohistochemistry-positive staining signals of AEV antigen were analysed by Image-Pro Plus software. A positive correlation between qPCR and IHC results was identified in most tissues. Our results provide an insight into the dynamic distribution of AEV in various tissues after infection. The distinct dynamic distribution of the viral genome in Korean quail in the early and late stages of infection suggests that AEV replication is affected by antibody levels and the maturity of the immune system of the host.
[Mh] Termos MeSH primário: Vírus da Encefalomielite Aviária/fisiologia
Infecções por Picornaviridae/veterinária
Doenças das Aves Domésticas/virologia
Codorniz
Tropismo Viral/fisiologia
[Mh] Termos MeSH secundário: Animais
Encéfalo/virologia
Bolsa de Fabricius/virologia
Intestinos/virologia
Fígado/virologia
Pâncreas/virologia
Infecções por Picornaviridae/virologia
Proventrículo/virologia
Baço/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3504-0


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[PMID]:28474290
[Au] Autor:Taunde P; Timbe P; Lucas AF; Tchamo C; Chilundo A; Dos Anjos F; Costa R; Bila CG
[Ad] Endereço:Faculty of Veterinary Medicine, Eduardo Mondlane University, Av. De Moçambique Km 1.5, Maputo, Mozambique.
[Ti] Título:Serological evidence of avian encephalomyelitis virus and Pasteurella multocida infections in free-range indigenous chickens in Southern Mozambique.
[So] Source:Trop Anim Health Prod;49(5):1047-1050, 2017 Jun.
[Is] ISSN:1573-7438
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A total of 398 serum samples from free-range indigenous chickens originating from four villages in Southern Mozambique were tested for the presence of avian encephalomyelitis virus (AEV) and Pasteurella multocida (PM) antibodies through commercial enzyme-linked immunosorbent assay (ELISA) kits. AEV and PM antibodies were detected in all villages surveyed. The proportion of positive samples was very high: 59.5% (95% confidence interval (CI) 51.7-67.7%) for AEV and 71.5% (95% CI 67.7-77.3%) for PM. Our findings revealed that these pathogens are widespread among free-range indigenous chickens in the studied villages and may represent a threat in the transmission of AEV and PM to wild, broiler or layer chickens in the region. Further research is warranted on epidemiology of circulating strains and impact of infection on the poultry industry.
[Mh] Termos MeSH primário: Galinhas
Infecções por Pasteurella/epidemiologia
Infecções por Picornaviridae/veterinária
Doenças das Aves Domésticas/epidemiologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antibacterianos/sangue
Anticorpos Antivirais/sangue
Vírus da Encefalomielite Aviária/isolamento & purificação
Ensaio de Imunoadsorção Enzimática/veterinária
Moçambique/epidemiologia
Infecções por Pasteurella/microbiologia
Pasteurella multocida/isolamento & purificação
Infecções por Picornaviridae/epidemiologia
Infecções por Picornaviridae/virologia
Doenças das Aves Domésticas/microbiologia
Doenças das Aves Domésticas/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Antibodies, Viral)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170506
[St] Status:MEDLINE
[do] DOI:10.1007/s11250-017-1304-x


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[PMID]:27624642
[Au] Autor:Pohjola L; Tammiranta N; Ek-Kommonen C; Soveri T; Hänninen ML; Fredriksson Ahomaa M; Huovilainen A
[Ad] Endereço:a Department of Production Animal Medicine, Faculty of Veterinary Medicine , University of Helsinki , Saarentaus , Finland.
[Ti] Título:A survey for selected avian viral pathogens in backyard chicken farms in Finland.
[So] Source:Avian Pathol;46(2):166-172, 2017 Apr.
[Is] ISSN:1465-3338
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Backyard poultry are regaining popularity in Europe and increased interest in the health and management of non-commercial farms has resulted. Furthermore, commercial poultry farm owners have become concerned about the risk represented by contagious avian diseases that nearby backyard poultry could transmit. Fifty-one voluntary backyard chicken farms were visited between October 2012 and January 2013. Blood samples and individual cloacal swabs were collected from 457 chickens. In 44 farms (86%), one or more of the tested chickens had antibodies against avian encephalomyelitis and chicken infectious anaemia viruses, 24 farms (47%) had chickens seropositive for infectious bronchitis virus, 10 farms (20%) had chickens seropositive for infectious bursal disease virus, six farms (12%) had chickens seropositive for infectious laryngotracheitis virus and two farms (5.4%) had chickens seropositive for avian influenza virus. No farms had chickens seropositive for Newcastle disease virus. Of the 51 farms, five (10%) had chickens positive for coronavirus reverse transcription polymerase chain reaction. A phylogenetic analysis showed that all backyard chicken coronaviruses collected were QX type infectious bronchitis viruses. All chickens tested for avian influenza and Newcastle disease viruses using real time reverse transcription polymerase chain reaction were negative. To our knowledge, there is no evidence to date to suggest that these diseases would have been transmitted between commercial and non-commercial flocks.
[Mh] Termos MeSH primário: Anticorpos Antivirais/sangue
Galinhas/virologia
Vírus de DNA/imunologia
Doenças das Aves Domésticas/virologia
Vírus de RNA/imunologia
[Mh] Termos MeSH secundário: Animais
Vírus da Anemia da Galinha/imunologia
Vírus da Anemia da Galinha/isolamento & purificação
Vírus de DNA/isolamento & purificação
Vírus da Encefalomielite Aviária/imunologia
Vírus da Encefalomielite Aviária/isolamento & purificação
Fazendas
Finlândia/epidemiologia
Herpesvirus Galináceo 1/imunologia
Herpesvirus Galináceo 1/isolamento & purificação
Vírus da Bronquite Infecciosa/imunologia
Vírus da Bronquite Infecciosa/isolamento & purificação
Vírus da Influenza A/genética
Vírus da Influenza A/imunologia
Vírus da Influenza A/isolamento & purificação
Vírus da Doença de Newcastle/genética
Vírus da Doença de Newcastle/imunologia
Vírus da Doença de Newcastle/isolamento & purificação
Filogenia
Doenças das Aves Domésticas/epidemiologia
Vírus de RNA/isolamento & purificação
Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária
Inquéritos e Questionários
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170606
[Lr] Data última revisão:
170606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160915
[St] Status:MEDLINE
[do] DOI:10.1080/03079457.2016.1232804


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[PMID]:27796116
[Au] Autor:Parsons NJ; Gous TA; Schaefer AM; Vanstreels RE
[Ad] Endereço:Southern African Foundation for the Conservation of Coastal Birds, Bloubergrant; Bayworld Centre for Research and Education, Port Elizabeth. nolaparsons@yahoo.co.uk.
[Ti] Título:Health evaluation of African penguins (Spheniscus demersus) in southern Africa.
[So] Source:Onderstepoort J Vet Res;83(1):e1-e13, 2016 Sep 20.
[Is] ISSN:2219-0635
[Cp] País de publicação:South Africa
[La] Idioma:eng
[Ab] Resumo:The African penguin (Spheniscus demersus) is an endangered seabird that breeds along the coast of Namibia and South Africa, and disease surveillance was identified as a priority for its conservation. Aiming for the establishment of baseline data on the presence of potential pathogens in this species, a comprehensive health assessment (blood smear examination, haematology, biochemistry and serology) was conducted on samples obtained from 578 African penguins at 11 breeding colonies and a rehabilitation centre. There were 68 penguins that were seropositive for at least one of seven pathogens tested: avian encephalomyelitis virus, avian infectious bronchitis virus, avian reovirus, infectious bursal disease virus, Newcastle disease virus, Mycoplasma gallisepticum and Mycoplasma synoviae. All samples were seronegative for avian influenza virus subtypes H5 and H7 and infectious laryngotracheitis virus. The apparent prevalence of Babesia sp. and Borrelia sp. in blood smears was consistent with previous studies. Babesia-infected individuals had a regenerative response of the erythrocytic lineage, an active inflammatory response and hepatic function impairment. These findings indicate that African penguins may be exposed to conservation-significant pathogens in the wild and encourage further studies aiming for the direct detection and/or isolation of these microorganisms.
[Mh] Termos MeSH primário: Doenças das Aves/epidemiologia
Spheniscidae
[Mh] Termos MeSH secundário: Animais
Babesia/isolamento & purificação
Doenças das Aves/sangue
Doenças das Aves/microbiologia
Vírus da Encefalomielite Aviária/isolamento & purificação
Vírus da Bronquite Infecciosa/isolamento & purificação
Mycoplasma/isolamento & purificação
Vírus da Doença de Newcastle/isolamento & purificação
Orthoreovirus Aviário/isolamento & purificação
África do Sul/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170206
[Lr] Data última revisão:
170206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161101
[St] Status:MEDLINE
[do] DOI:10.4102/ojvr.v83i1.1147


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[PMID]:26493005
[Au] Autor:Yu XH; Zhao J; Qin XH; Zhang GZ
[Ad] Endereço:Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
[Ti] Título:Serological evidence of avian encephalomyelitis virus infection associated with vertical transmission in chicks.
[So] Source:Biologicals;43(6):512-4, 2015 Nov.
[Is] ISSN:1095-8320
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Avian encephalomyelitis virus (AEV) can be transmitted both horizontally and vertically. In the present study, we report a typical case of AEV infection in broiler breeder chickens and their progeny identified by clinical survey of the disease, antibody detection, and reverse transcription (RT)-polymerase chain reaction (PCR) assay.
[Mh] Termos MeSH primário: Anticorpos Antivirais/sangue
Galinhas/virologia
Vírus da Encefalomielite Aviária/imunologia
Transmissão Vertical de Doença Infecciosa
Infecções por Picornaviridae/veterinária
Doenças das Aves Domésticas/transmissão
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/biossíntese
Antígenos Virais/imunologia
Encéfalo/virologia
Proteínas do Capsídeo/imunologia
Galinhas/imunologia
China/epidemiologia
Surtos de Doenças
Vírus da Encefalomielite Aviária/isolamento & purificação
Ensaio de Imunoadsorção Enzimática
Feminino
Infecções por Picornaviridae/sangue
Infecções por Picornaviridae/epidemiologia
Infecções por Picornaviridae/transmissão
Doenças das Aves Domésticas/sangue
Doenças das Aves Domésticas/epidemiologia
Reação em Cadeia da Polimerase Via Transcriptase Reversa
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Antigens, Viral); 0 (Capsid Proteins)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:151111
[Lr] Data última revisão:
151111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151024
[St] Status:MEDLINE


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[PMID]:25647602
[Au] Autor:Ingram DR; Miller DL; Baldwin CA; Turco J; Lockhart JM
[Ad] Endereço:1 The University of Georgia, College of Veterinary Medicine, Veterinary Diagnostic and Investigational Laboratory, 43 Brighton Rd., Tifton, Georgia 31793, USA.
[Ti] Título:Serologic survey of wild turkeys (Meleagris gallopavo) and evidence of exposure to avian encephalomyelitis virus in Georgia and Florida, USA.
[So] Source:J Wildl Dis;51(2):374-9, 2015 Apr.
[Is] ISSN:1943-3700
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Wild Turkeys (Meleagris gallopavo) are susceptible to many of the same diseases as domestic turkeys. Before 2005, most Wild Turkeys in southern Georgia, US, had little or no exposure to commercial poultry operations. As part of a pathogen survey examining the effects of commercial poultry on Wild Turkeys, samples were collected from Wild Turkeys from March 2005 through May 2008. The turkeys were collected from 13 counties in southern Georgia and Madison County, Florida, and tested for antibodies to various pathogens of poultry. Three (13%) of the turkeys were positive for antibodies to Salmonella. Thirteen turkeys (54%) were positive for Newcastle disease virus antibodies, and 15 turkeys (63%) were positive for antibodies to reticuloendotheliosis virus. One turkey (4%) from Madison County was positive for avian encephalomyelitis virus antibodies.
[Mh] Termos MeSH primário: Doenças das Aves/virologia
Vírus da Encefalomielite Aviária
Infecções por Picornaviridae/veterinária
Perus
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/sangue
Doenças das Aves/sangue
Florida/epidemiologia
Georgia/epidemiologia
Vírus da Doença de Newcastle/imunologia
Infecções por Picornaviridae/sangue
Infecções por Picornaviridae/epidemiologia
Infecções por Picornaviridae/virologia
Vírus da Reticuloendoteliose Aviária/imunologia
Salmonelose Animal/sangue
Salmonelose Animal/epidemiologia
Estudos Soroepidemiológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:150410
[Lr] Data última revisão:
150410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150204
[St] Status:MEDLINE
[do] DOI:10.7589/2013-07-169


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[PMID]:24880065
[Au] Autor:Liu Q; Yang Z; Hao H; Cheng S; Fan W; Du E; Xiao S; Wang X; Zhang S
[Ad] Endereço:College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi Province, China.
[Ti] Título:Development of a SYBR Green real-time RT-PCR assay for the detection of avian encephalomyelitis virus.
[So] Source:J Virol Methods;206:46-50, 2014 Sep.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Avian encephalomyelitis virus (AEV) causes epidemic diseases in poultry worldwide. A SYBR Green real-time reverse transcription-polymerase chain reaction (rRT-PCR) assay was developed for the rapid detection and quantitation of AEV in this study. A pair of specific primers was designed in the highly conserved VP1 gene of this virus. When comparing this assay with conventional RT-PCR, the rRT-PCR assay was 100 times more sensitive and could detect levels as low as 10 standard DNA copies of the AEV SX strain. The specificity of this technique was evaluated in five other avian pathogens. The AEV RNA was detected as early as three days post-infection in chicken embryos. All 18 clinical chicken brains collected from an AEV outbreak in Northwestern China were detected to be positive (100%) using the rRT-PCR assay. However, only 5 of the 18 samples were positive (28%) using the conventional RT-PCR. The results were confirmed by virus isolation in chicken embryos. This high sensitivity, specificity, and simplicity of the SYBR Green rRT-PCR approach can be a more effective method than the conventional one for AEV diagnosis and surveillance.
[Mh] Termos MeSH primário: Vírus da Encefalomielite Aviária/isolamento & purificação
Compostos Orgânicos/metabolismo
Infecções por Picornaviridae/veterinária
Doenças das Aves Domésticas/diagnóstico
Reação em Cadeia da Polimerase em Tempo Real/métodos
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
Coloração e Rotulagem/métodos
[Mh] Termos MeSH secundário: Animais
Galinhas
China
Primers do DNA/genética
Vírus da Encefalomielite Aviária/genética
Infecções por Picornaviridae/diagnóstico
Infecções por Picornaviridae/virologia
Doenças das Aves Domésticas/virologia
Sensibilidade e Especificidade
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA Primers); 0 (Organic Chemicals); 163795-75-3 (SYBR Green I)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:140721
[Lr] Data última revisão:
140721
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140601
[St] Status:MEDLINE


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[PMID]:23334009
[Au] Autor:Glanville TD; Ahn HK; Richard TL; Harmon JD; Reynolds DL; Akinc S
[Ad] Endereço:Dept. of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA 50011-3080, United States. tglanvil@iastate.edu
[Ti] Título:Effect of envelope material on biosecurity during emergency bovine mortality composting.
[So] Source:Bioresour Technol;130:543-51, 2013 Feb.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The biosecurity of composting as an emergency disposal method for cattle mortalities caused by disease was evaluated by conducting full-scale field trials begun during three different seasons and using three different envelope materials. Process biosecurity was significantly affected by the envelope material used to construct the composting matrix. Internal temperatures met USEPA Class A time/temperature criteria for pathogen reduction in 89%, 67%, and 22%, respectively of seasonal test units constructed with corn silage, straw/manure, or ground cornstalks. In trials begun in the winter, survival times of vaccine strains of avian encephalomyelitis and Newcastle disease virus were noticeably shorter in silage test units than in the other two materials, but during summer/spring trials survival times in ground cornstalk and straw/manure test units were similar to those in test units constructed with silage.
[Mh] Termos MeSH primário: Doenças dos Bovinos/prevenção & controle
Emergências
Microbiologia do Solo
Inativação de Vírus
[Mh] Termos MeSH secundário: Animais
Bovinos
Vírus da Encefalomielite Aviária/fisiologia
Temperatura Alta
Esterco
Vírus da Doença de Newcastle/fisiologia
Oxigênio/análise
Estações do Ano
Solo/análise
Zea mays
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Manure); 0 (Soil); S88TT14065 (Oxygen)
[Em] Mês de entrada:1309
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130122
[St] Status:MEDLINE


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[PMID]:22015139
[Au] Autor:Kurian A; Neumann EJ; Hall WF; Marks D
[Ad] Endereço:EpiCentre, Institute of Veterinary, Animal and Veterinary Biosciences, Massey University, Tennent Drive, Palmerston North 4442, New Zealand.
[Ti] Título:Effects of blood sample mishandling on ELISA results for infectious bronchitis virus, avian encephalomyelitis virus and chicken anaemia virus.
[So] Source:Vet J;192(3):378-81, 2012 Jun.
[Is] ISSN:1532-2971
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study determined the effect of sample mishandling on the performance of ELISAs for detection of antibodies against infectious bronchitis virus (IBV), avian encephalomyelitis virus (AEV) and chicken anaemia virus (CAV) in the serum of chickens. The effects of five different sample mishandling treatments were assessed: heat treatment, repetitive freezing and thawing and three levels of severity of haemolysis. These mishandling treatments simulated different conditions that might occur during routine blood collection, transport or storage in a clinical practice setting. Each mishandling treatment was experimentally applied under laboratory conditions and then samples were assayed for antibodies against IBV, AEV and CAV using commercial ELISA kits. Severe haemolysis had the most consistent detrimental effect on ELISA performance, producing results that were significantly different from the reference standard in all three ELISAs, although the direction of the effect varied (less positive for the IBV and CAV assays; more positive for the AEV assay). Moderate levels of haemolysis had a similar, but less consistent, effect to that of severe haemolysis, producing results that were significantly different from the reference standard only for the IBV (less positive) and AEV (more positive) ELISAs. Repetitive freeze-thawing also produced a significant effect on ELISA results for IBV (less positive) and AEV (more positive). The IBV ELISA appeared to be most susceptible to the effects of serum maltreatment. The findings from this study suggest that unpredictable variation in the results of ELISAs can occur due to different sample mishandling treatments.
[Mh] Termos MeSH primário: Coleta de Amostras Sanguíneas/veterinária
Vírus da Anemia da Galinha/imunologia
Galinhas
Vírus da Encefalomielite Aviária/imunologia
Ensaio de Imunoadsorção Enzimática/veterinária
Vírus da Bronquite Infecciosa/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/sangue
Coleta de Amostras Sanguíneas/métodos
Infecções por Circoviridae/sangue
Infecções por Circoviridae/diagnóstico
Infecções por Circoviridae/veterinária
Infecções por Circoviridae/virologia
Infecções por Coronavirus/sangue
Infecções por Coronavirus/diagnóstico
Infecções por Coronavirus/veterinária
Infecções por Coronavirus/virologia
Congelamento
Hemólise
Infecções por Picornaviridae/sangue
Infecções por Picornaviridae/diagnóstico
Infecções por Picornaviridae/veterinária
Infecções por Picornaviridae/virologia
Doenças das Aves Domésticas/sangue
Doenças das Aves Domésticas/diagnóstico
Doenças das Aves Domésticas/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1210
[Cu] Atualização por classe:120611
[Lr] Data última revisão:
120611
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:111022
[St] Status:MEDLINE
[do] DOI:10.1016/j.tvjl.2011.08.028


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[PMID]:19468944
[Au] Autor:Welchman Dde B; Cox WJ; Gough RE; Wood AM; Smyth VJ; Todd D; Spackman D
[Ad] Endereço:VLA Winchester, Winchester, UK. d.welchman@vla.defra.gsi.gov.uk
[Ti] Título:Avian encephalomyelitis virus in reared pheasants: a case study.
[So] Source:Avian Pathol;38(3):251-6, 2009 Jun.
[Is] ISSN:1465-3338
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An outbreak of neurological disease occurred in pheasant chicks on a game farm in 2007. The disease was first seen in the 10th hatching of chicks on the farm. Affected chicks showed trembling and incoordination from the time of hatching, and subsequently blindness and cataract formation was seen in some of the affected chicks at 3 weeks of age. The peak mortality and culling figure was 21.0% in the worst affected hatch, compared with a maximum of 11.7% in the first nine hatches. No further cases were evident by 7.5 weeks of age. Histopathological examination showed a moderate acute encephalomyelitis in some, but not all, of the chicks with neurological signs. The clinical presentation and histopathological findings were typical of vertically transmitted avian encephalomyelitis as seen in chickens, although avian encephalomyelitis virus could not be detected in inoculated embryonated chicken eggs. However, serological testing by enzyme-linked immunosorbent assay for antibodies to the virus was positive in four of five affected 3-week-old birds and in 23 out of 29 adult breeding birds, and reverse transcriptase-polymerase chain reaction testing of RNA extracted from brain and pancreas tissue of affected chicks yielded nucleotide sequences aligned 82% and 83% with three avian encephalomyelitis sequences in a sequence database. The evidence suggested that the neurological disease was attributable to infection with a strain of avian encephalomyelitis virus that appeared to have entered the flock at the start of the breeding season, and was possibly introduced by carrier pheasants brought on to the farm early in the season.
[Mh] Termos MeSH primário: Surtos de Doenças/veterinária
Vírus da Encefalomielite Aviária/genética
Infecções por Picornaviridae/veterinária
Doenças das Aves Domésticas/epidemiologia
Doenças das Aves Domésticas/virologia
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Ensaio de Imunoadsorção Enzimática/veterinária
Dados de Sequência Molecular
Infecções por Picornaviridae/epidemiologia
Infecções por Picornaviridae/patologia
Aves Domésticas
Doenças das Aves Domésticas/patologia
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Análise de Sequência de DNA
Reino Unido/epidemiologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:0908
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090527
[St] Status:MEDLINE
[do] DOI:10.1080/03079450902912168



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