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[PMID]:28152384
[Au] Autor:Gutkoska J; LaRocco M; Ramirez-Medina E; de Los Santos T; Lawrence P
[Ad] Endereço:Plum Island Animal Disease Center Foreign Animal Disease Research Unit (FADRU) Agricultural Research Service (ARS), United States Department of Agriculture (USDA), 40550 Route 25, Orient Point, NY 11957, United States.
[Ti] Título:Host microRNA-203a Is antagonistic to the progression of foot-and-mouth disease virus infection.
[So] Source:Virology;504:52-62, 2017 Apr.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sam68 was previously shown to be a critical host factor for foot-and-mouth disease virus (FMDV) replication. MicroRNA (miR) miR-203a is reportedly a negative regulator of Sam68 expression both in vitro and in vivo. Here, transfection of miR-203a-3p and miR-203a-5p mimics separately and in combination in a porcine cell line followed by FMDV infection resulted in diminished viral protein synthesis and a 4 and 6log reduction in virus titers relative to negative controls, respectively. Unexpectedly, Sam68 expression was increased by miR-203a-5p transfection, but not miR-203a-3p. miR-203a-5p also down-regulated Survivin expression, which was predicted to play a role in FMDV infection. Moreover, miR-203a-5p but not miR-203a-3p affected a reduction in FMDV viral RNA. These effects were not replicated with a related Picornavirus, suggesting FMDV specificity. Importantly, miR-203a-3p and miR-203a-5p impaired FMDV infection across multiple FMDV serotypes. We concluded that miR-203a-3p and miR-203a-5p represent attractive potential naturally occurring bio-therapeutics against FMDV.
[Mh] Termos MeSH primário: Vírus da Febre Aftosa/crescimento & desenvolvimento
Vírus da Febre Aftosa/genética
MicroRNAs/genética
Carga Viral/genética
Replicação Viral/genética
[Mh] Termos MeSH secundário: Animais
Bovinos
Linhagem Celular
Progressão da Doença
Cães
Enterovirus Bovino/genética
Células Madin Darby de Rim Canino
Biossíntese de Proteínas/genética
Interferência de RNA
RNA Interferente Pequeno/genética
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs); 0 (RNA, Small Interfering)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170523
[Lr] Data última revisão:
170523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170203
[St] Status:MEDLINE


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[PMID]:28095784
[Au] Autor:Tsuchiaka S; Rahpaya SS; Otomaru K; Aoki H; Kishimoto M; Naoi Y; Omatsu T; Sano K; Okazaki-Terashima S; Katayama Y; Oba M; Nagai M; Mizutani T
[Ad] Endereço:The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagito, Gifu-shi, Gifu, 501-1193, Japan.
[Ti] Título:Identification of a novel bovine enterovirus possessing highly divergent amino acid sequences in capsid protein.
[So] Source:BMC Microbiol;17(1):18, 2017 Jan 17.
[Is] ISSN:1471-2180
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Bovine enterovirus (BEV) belongs to the species Enterovirus E or F, genus Enterovirus and family Picornaviridae. Although numerous studies have identified BEVs in the feces of cattle with diarrhea, the pathogenicity of BEVs remains unclear. Previously, we reported the detection of novel kobu-like virus in calf feces, by metagenomics analysis. In the present study, we identified a novel BEV in diarrheal feces collected for that survey. Complete genome sequences were determined by deep sequencing in feces. Secondary RNA structure analysis of the 5' untranslated region (UTR), phylogenetic tree construction and pairwise identity analysis were conducted. RESULTS: The complete genome sequences of BEV were genetically distant from other EVs and the VP1 coding region contained novel and unique amino acid sequences. We named this strain as BEV AN12/Bos taurus/JPN/2014 (referred to as BEV-AN12). According to genome analysis, the genome length of this virus is 7414 nucleotides excluding the poly (A) tail and its genome consists of a 5'UTR, open reading frame encoding a single polyprotein, and 3'UTR. The results of secondary RNA structure analysis showed that in the 5'UTR, BEV-AN12 had an additional clover leaf structure and small stem loop structure, similarly to other BEVs. In pairwise identity analysis, BEV-AN12 showed high amino acid (aa) identities to Enterovirus F in the polyprotein, P2 and P3 regions (aa identity ≥82.4%). Therefore, BEV-AN12 is closely related to Enterovirus F. However, aa sequences in the capsid protein regions, particularly the VP1 encoding region, showed significantly low aa identity to other viruses in genus Enterovirus (VP1 aa identity ≤58.6%). In addition, BEV-AN12 branched separately from Enterovirus E and F in phylogenetic trees based on the aa sequences of P1 and VP1, although it clustered with Enterovirus F in trees based on sequences in the P2 and P3 genome region. CONCLUSIONS: We identified novel BEV possessing highly divergent aa sequences in the VP1 coding region in Japan. According to species definition, we proposed naming this strain as "Enterovirus K", which is a novel species within genus Enterovirus. Further genomic studies are needed to understand the pathogenicity of BEVs.
[Mh] Termos MeSH primário: Sequência de Aminoácidos/genética
Proteínas do Capsídeo/genética
Doenças dos Bovinos/virologia
Enterovirus Bovino/genética
Enterovirus Bovino/isolamento & purificação
[Mh] Termos MeSH secundário: Regiões 3' não Traduzidas/genética
Regiões 5' não Traduzidas/genética
Animais
Sequência de Bases
Bovinos
Diarreia/veterinária
Infecções por Enterovirus/virologia
Enterovirus Bovino/classificação
Enterovirus Bovino/patogenicidade
Fezes/virologia
Genoma Viral
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Japão
Metagenômica/métodos
Fases de Leitura Aberta/genética
Filogenia
RNA Viral/química
RNA Viral/genética
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3' Untranslated Regions); 0 (5' Untranslated Regions); 0 (Capsid Proteins); 0 (RNA, Viral); 0 (Viral Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170119
[St] Status:MEDLINE
[do] DOI:10.1186/s12866-016-0923-0


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[PMID]:27620499
[Au] Autor:Ellgaard TW; Bindslev L; Kamstrup S
[Ad] Endereço:Dept. 609, Biopharm Purification Development & Virology, Novo Nordisk A/S, Hagedornsvej 1, DK-2820 Gentofte, Denmark. Electronic address: tryw@novonordisk.com.
[Ti] Título:Evaluation of the virus clearance capacity and robustness of the manufacturing process for the recombinant factor VIII protein, turoctocog alfa.
[So] Source:Protein Expr Purif;129:94-100, 2017 Jan.
[Is] ISSN:1096-0279
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Turoctocog alfa is a B-domain-truncated recombinant factor VIII protein produced in a Chinese hamster ovary (CHO) cell line. The aim of this study was to evaluate the virus clearance capacity and robustness of the turoctocog alfa purification process. Virus clearance evaluation studies were conducted utilising a scaled-down version of the manufacturing process. Total virus clearance was evaluated using the ecotropic murine leukaemia virus (eMuLV) as a model for non-infectious retrovirus-like particles (RVLPs) and certain enveloped viruses. Other viruses utilised included: infectious bovine rhinotracheitis (IBRV), minute virus of mice (MVM), bovine enterovirus (BEV) and Reo-3 virus (Reo-3). Robust clearance of all model viruses was demonstrated with either new or reused resins. Overall, virus reduction factors were: >18.0 log (eMuLV); 11.0 log (MVM); >11.8 log (Reo-3; >5.0 log using nanofiltration); >15.3 log (BEV) and >12.7 log (IBRV). Taken together, these values demonstrate that the purification process for turoctocog alfa effectively removes a range of enveloped and non-enveloped viruses of different physicochemical properties and sizes.
[Mh] Termos MeSH primário: Enterovirus Bovino
Fator VIII/isolamento & purificação
Herpesvirus Bovino 1
Vírus da Leucemia Murina
Vírus Miúdo do Camundongo
Inativação de Vírus
[Mh] Termos MeSH secundário: Animais
Células CHO
Bovinos
Cricetinae
Cricetulus
Fator VIII/biossíntese
Fator VIII/genética
Camundongos
Proteínas Recombinantes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (F8 protein, human); 0 (Recombinant Proteins); 9001-27-8 (Factor VIII)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160914
[St] Status:MEDLINE


  4 / 47 MEDLINE  
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[PMID]:26811239
[Au] Autor:Kosoltanapiwat N; Yindee M; Chavez IF; Leaungwutiwong P; Adisakwattana P; Singhasivanon P; Thawornkuno C; Thippornchai N; Rungruengkitkun A; Soontorn J; Pearsiriwuttipong S
[Ad] Endereço:Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. nathamon.kos@mahidol.ac.th.
[Ti] Título:Genetic variations in regions of bovine and bovine-like enteroviral 5'UTR from cattle, Indian bison and goat feces.
[So] Source:Virol J;13:13, 2016 Jan 25.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Bovine enteroviruses (BEV) are members of the genus Enterovirus in the family Picornaviridae. They are predominantly isolated from cattle feces, but also are detected in feces of other animals, including goats and deer. These viruses are found in apparently healthy animals, as well as in animals with clinical signs and several studies reported recently suggest a potential role of BEV in causing disease in animals. In this study, we surveyed the presence of BEV in domestic and wild animals in Thailand, and assessed their genetic variability. METHODS: Viral RNA was extracted from fecal samples of cattle, domestic goats, Indian bison (gaurs), and deer. The 5' untranslated region (5'UTR) was amplified by nested reverse transcription-polymerase chain reaction (RT-PCR) with primers specific to BEV 5'UTR. PCR products were sequenced and analyzed phylogenetically using the neighbor-joining algorithm to observe genetic variations in regions of the bovine and bovine-like enteroviral 5'UTR found in this study. RESULTS: BEV and BEV-like sequences were detected in the fecal samples of cattle (40/60, 67 %), gaurs (3/30, 10 %), and goats (11/46, 24 %). Phylogenetic analyses of the partial 5'UTR sequences indicated that different BEV variants (both EV-E and EV-F species) co-circulated in the domestic cattle, whereas the sequences from gaurs and goats clustered according to the animal species, suggesting that these viruses are host species-specific. CONCLUSIONS: Varieties of BEV and BEV-like 5'UTR sequences were detected in fecal samples from both domestic and wild animals. To our knowledge, this is the first report of the genetic variability of BEV in Thailand.
[Mh] Termos MeSH primário: Regiões 5´ não Traduzidas
Enterovirus Bovino/classificação
Enterovirus Bovino/genética
Variação Genética
[Mh] Termos MeSH secundário: Animais
Bison
Bovinos
Enterovirus Bovino/isolamento & purificação
Fezes/virologia
Geografia
Cabras
Filogenia
RNA Viral
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (5' Untranslated Regions); 0 (RNA, Viral)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160127
[St] Status:MEDLINE
[do] DOI:10.1186/s12985-016-0468-8


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[PMID]:26738285
[Au] Autor:Zhu T; Zhao G; Shen F; Hou Peili; Wang H; Li J; He H
[Ti] Título:[Establishment and Preliminary Application of the SYBR Green I Real-time PCR Assay for Detection of the Bovine Enterovirus].
[So] Source:Bing Du Xue Bao;31(5):488-93, 2015 Sep.
[Is] ISSN:1000-8721
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:The bovine enterovirus (BEV) is a pathogen found the digestive tracts of cattle. Recently, the BEV was discovered in cattle in a province in China. A rapid and effective detection method for the BEV is essential. An assay was carried out using two specific primers designed to amplify a highly conserved sequence of the 3D gene. A recombinant plasmid containing the target gene 3D was constructed as a standard control. The limit of detection of the reaction was 7.13 x 10(1) plasmid copies/µL of initial templates, which was tenfold more sensitive than the conventional reverse-transcription-polymerase chain reaction (RT-PCR). Moreover, the assay was highly specific because all negative controls and other viruses of clinical relevance did not develop positive results. Assay performance on field samples was evaluated on 44 (41 diarrhea and 3 aerosol) samples and compared with the conventional RT-PCR assay. Sixteen diarrhea samples were positive (16/41, 39. 02%) and 3 aerosol samples were positive (3/3, 100%). Preliminary results for clinical detection showed that the SYBR Green I real-time PCR assay was highly sensitive, specific and reproducible. The robustness and high-throughput performance of the developed assay make it a powerful tool in diagnostic applications for epidemics and in BEV research.
[Mh] Termos MeSH primário: Doenças dos Bovinos/virologia
Infecções por Enterovirus/veterinária
Enterovirus Bovino/isolamento & purificação
Reação em Cadeia da Polimerase em Tempo Real/métodos
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos/diagnóstico
Primers do DNA/química
Primers do DNA/genética
Infecções por Enterovirus/diagnóstico
Infecções por Enterovirus/virologia
Enterovirus Bovino/genética
Compostos Orgânicos/química
Sensibilidade e Especificidade
[Pt] Tipo de publicação:ENGLISH ABSTRACT; EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA Primers); 0 (Organic Chemicals); 163795-75-3 (SYBR Green I)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:161021
[Lr] Data última revisão:
161021
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160108
[St] Status:MEDLINE


  6 / 47 MEDLINE  
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[PMID]:26586212
[Au] Autor:Sobhy NM; Mor SK; Mohammed ME; Bastawecy IM; Fakhry HM; Youssef CR; Abouzeid NZ; Goyal SM
[Ad] Endereço:Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia 44511, Egypt.
[Ti] Título:Isolation and molecular characterization of bovine enteroviruses in Egypt.
[So] Source:Vet J;206(3):317-21, 2015 Dec.
[Is] ISSN:1532-2971
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Enteroviruses belong to the Picornaviridae family and infect a wide range of mammals including cattle. Bovine enterovirus (BEV) has recently been reclassified into E and F serotypes. BEV was first isolated in Egypt in 1966 although it has been known in other countries since the 1950s. In this study, BEV-F2 was isolated from calves with severe diarrhea and the isolated viruses were subjected to molecular characterization. Illumina sequencing of one of the isolates revealed the presence of a complete BEV-F genome sequence. The phylogenetic analysis revealed nucleotide substitutions along the genome in comparison with other known strains of BEV-F (HQ663846, AY508697 and DQ092795). Two primer sets were designed from the 3D and 5'NTR regions and used for the examination of the remaining isolates, which were confirmed to be of the BEV-F2 serotype. The availability of the complete genome sequence of this virus adds to the sequence database of the members of Picornaviridae and should be useful in future molecular studies of BEV.
[Mh] Termos MeSH primário: Infecções por Enterovirus/veterinária
Enterovirus Bovino/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Bovinos
Diarreia/veterinária
Diarreia/virologia
Egito
Infecções por Enterovirus/virologia
Enterovirus Bovino/classificação
Enterovirus Bovino/genética
Fezes/virologia
Genoma Viral
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151121
[St] Status:MEDLINE


  7 / 47 MEDLINE  
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[PMID]:25286661
[Au] Autor:Malaczewska J; Siwicki AK
[Ti] Título:Commercial metal-based nanocolloids--lack of virucidal activity against ECBO virus.
[So] Source:Pol J Vet Sci;17(3):507-9, 2014.
[Is] ISSN:1505-1773
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Metallic nanoparticles, mainly silver ones, have been widely used as antibacterial agents, and some studies shown they also exert direct antiviral activity against both enveloped and non-enveloped viruses. The objective of this study has been to evaluate the virucidal activity of commercial silver, gold, copper and platinum nanocolloids, recommended by the manufacturer as antimicrobials, against the ECBO virus, according to Polish Standard PN-EN 14675:2006. The highest experimentally observed decrease in the viral load was 0.875 log, which--when contrasted with the reduction in virus titre of at least 4 log expected from disinfectants--indicates that none of the analyzed nanocolloids had a disinfectant power towards the ECBO virus under the conditions defined by the standard.
[Mh] Termos MeSH primário: Antivirais/química
Antivirais/farmacologia
Coloides/farmacologia
Enterovirus Bovino/efeitos dos fármacos
Nanopartículas Metálicas/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Coloides/química
Cães
Nanopartículas Metálicas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Colloids)
[Em] Mês de entrada:1411
[Cu] Atualização por classe:141007
[Lr] Data última revisão:
141007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141008
[St] Status:MEDLINE


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[PMID]:25059211
[Au] Autor:Abd-Elmaksoud S; Spencer SK; Gerba CP; Tamimi AH; Jokela WE; Borchardt MA
[Ad] Endereço:Environmental Virology Laboratory, Department of Water Pollution Research, National Research Centre, Cairo, Egypt.
[Ti] Título:Simultaneous Concentration of Bovine Viruses and Agricultural Zoonotic Bacteria from Water Using Sodocalcic Glass Wool Filters.
[So] Source:Food Environ Virol;6(4):253-9, 2014 Dec.
[Is] ISSN:1867-0342
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Infiltration and runoff from manured agricultural fields can result in livestock pathogens reaching groundwater and surface waters. Here, we measured the effectiveness of glass wool filters to simultaneously concentrate enteric viruses and bacteria of bovine origin from water. The recovery efficiencies were determined for bovine viral diarrhea virus types 1 and 2, bovine rotavirus group A, bovine coronavirus, poliovirus Sabin III, toxigenic Escherichia coli ,and Campylobacter jejuni seeded into water with three different turbidity levels (0.5, 215, and 447 NTU). Twenty liters of dechlorinated tap water (pH 7) were seeded with the test organisms, and then passed through a glass wool filter using a peristaltic pump (flow rate = 1 liter min(-1)). Retained organisms were eluted from the filters by passing beef extract-glycine buffer (pH 9.5) in the direction opposite of sample flow. Recovered organisms were enumerated by qPCR except for C. jejuni, which was quantified by culture. Mean recovery efficiencies ranged from 55 to 33% for the bacteria and 58 to 16% for the viruses. Using bootstrapping techniques combined with Analysis of Variance, recovery efficiencies were found to differ among the pathogen types tested at the two lowest turbidity levels; however, for a given pathogen type turbidity did not affect recovery except for C. jejuni. Glass wool filtration is a cost-effective method for concentrating several waterborne pathogens of bovine origin simultaneously, although recovery may be low for some specific taxa such as bovine viral diarrhea virus 1.
[Mh] Termos MeSH primário: Campylobacter jejuni/isolamento & purificação
Coronavirus Bovino/isolamento & purificação
Vírus da Diarreia Viral Bovina/isolamento & purificação
Escherichia coli Enterotoxigênica/isolamento & purificação
Enterovirus Bovino/isolamento & purificação
Rotavirus/isolamento & purificação
Águas Residuais/microbiologia
[Mh] Termos MeSH secundário: Análise de Variância
Animais
Carga Bacteriana
Bovinos
Indústria de Laticínios
Monitoramento Ambiental/métodos
Filtração
Floculação
Vidro/química
Reprodutibilidade dos Testes
Carga Viral
Águas Residuais/virologia
Zoonoses/microbiologia
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Waste Water)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140726
[St] Status:MEDLINE
[do] DOI:10.1007/s12560-014-9159-z


  9 / 47 MEDLINE  
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[PMID]:24830424
[Au] Autor:Zhu L; Xing Z; Gai X; Li S; San Z; Wang X
[Ad] Endereço:College of Veterinary Medicine, Jilin University, Changchun, Jilin, China.
[Ti] Título:Identification of a novel enterovirus E isolates HY12 from cattle with severe respiratory and enteric diseases.
[So] Source:PLoS One;9(5):e97730, 2014.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, a virus strain designated as HY12 was isolated from cattle with a disease of high morbidity and mortality in Jilin province. Biological and physiochemical properties showed that HY12 isolates is cytopathic with an extremely high infectivity. HY12 is resistant to treatment of organic solvent and acid, and unstable at 60°C for 1 h. Electron microscopy observation revealed the virus is an approximately 22-28 nm in diameter. The complete genome sequence of HY12 consists of 7416 nucleotides, with a typical picornavirus genome organization including a 5'-untranslated region (UTR), a large single ORF encoding a polyprotein of 2176 amino acids, and a 3'-UTR. Phylogenetic analysis clustered HY12 isolates to a new serotype/genotype within the clade of enterovirus E (formerly BEV-A). Alignment analysis revealed a unique insertion of 2 amino acid residues (NF) at the C-terminal of VP1 protein between aa 825 and 826, and several rare mutations in VP1 and VP4 of HY12 isolates in relation to known bovine enterovirus (BEV) strains. This is the first report of an enterovirus E in China, which is potentially associated with an outbreak in cattle with severe respiratory and enteric diseases.
[Mh] Termos MeSH primário: Doenças dos Bovinos/virologia
Enterite/veterinária
Infecções por Enterovirus/veterinária
Enterovirus Bovino/genética
Infecções Respiratórias/veterinária
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Proteínas do Capsídeo/química
Bovinos
Linhagem Celular
Enterite/virologia
Infecções por Enterovirus/virologia
Enterovirus Bovino/classificação
Enterovirus Bovino/isolamento & purificação
Genótipo
Dados de Sequência Molecular
Filogenia
Infecções Respiratórias/virologia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Capsid Proteins)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140517
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0097730


  10 / 47 MEDLINE  
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[PMID]:24816094
[Au] Autor:Owen RL; Paterson N; Axford D; Aishima J; Schulze-Briese C; Ren J; Fry EE; Stuart DI; Evans G
[Ad] Endereço:Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, England.
[Ti] Título:Exploiting fast detectors to enter a new dimension in room-temperature crystallography.
[So] Source:Acta Crystallogr D Biol Crystallogr;70(Pt 5):1248-56, 2014 May.
[Is] ISSN:1399-0047
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A departure from a linear or an exponential intensity decay in the diffracting power of protein crystals as a function of absorbed dose is reported. The observation of a lag phase raises the possibility of collecting significantly more data from crystals held at room temperature before an intolerable intensity decay is reached. A simple model accounting for the form of the intensity decay is reintroduced and is applied for the first time to high frame-rate room-temperature data collection.
[Mh] Termos MeSH primário: Cristalografia por Raios X/métodos
[Mh] Termos MeSH secundário: Cristalografia por Raios X/instrumentação
Enterovirus Bovino/química
Vírus da Febre Aftosa/química
Modelos Teóricos
Proteínas/química
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Proteins)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140513
[St] Status:MEDLINE
[do] DOI:10.1107/S1399004714005379



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