Base de dados : MEDLINE
Pesquisa : B04.820.565.284.600 [Categoria DeCS]
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  1 / 137 MEDLINE  
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[PMID]:29324778
[Au] Autor:Tsuchiaka S; Naoi Y; Imai R; Masuda T; Ito M; Akagami M; Ouchi Y; Ishii K; Sakaguchi S; Omatsu T; Katayama Y; Oba M; Shirai J; Satani Y; Takashima Y; Taniguchi Y; Takasu M; Madarame H; Sunaga F; Aoki H; Makino S; Mizutani T; Nagai M
[Ad] Endereço:Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
[Ti] Título:Genetic diversity and recombination of enterovirus G strains in Japanese pigs: High prevalence of strains carrying a papain-like cysteine protease sequence in the enterovirus G population.
[So] Source:PLoS One;13(1):e0190819, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To study the genetic diversity of enterovirus G (EV-G) among Japanese pigs, metagenomics sequencing was performed on fecal samples from pigs with or without diarrhea, collected between 2014 and 2016. Fifty-nine EV-G sequences, which were >5,000 nucleotides long, were obtained. By complete VP1 sequence analysis, Japanese EV-G isolates were classified into G1 (17 strains), G2 (four strains), G3 (22 strains), G4 (two strains), G6 (two strains), G9 (six strains), G10 (five strains), and a new genotype (one strain). Remarkably, 16 G1 and one G2 strain identified in diarrheic (23.5%; four strains) or normal (76.5%; 13 strains) fecal samples possessed a papain-like cysteine protease (PL-CP) sequence, which was recently found in the USA and Belgium in the EV-G genome, at the 2C-3A junction site. This paper presents the first report of the high prevalence of viruses carrying PL-CP in the EV-G population. Furthermore, possible inter- and intragenotype recombination events were found among EV-G strains, including G1-PL-CP strains. Our findings may advance the understanding of the molecular epidemiology and genetic evolution of EV-Gs.
[Mh] Termos MeSH primário: Infecções por Enterovirus/virologia
Enterovirus Suínos/genética
Variação Genética
Recombinação Genética
[Mh] Termos MeSH secundário: Animais
Proteínas do Capsídeo/genética
Cisteína Proteases/genética
Infecções por Enterovirus/epidemiologia
Enterovirus Suínos/enzimologia
Fezes/virologia
Japão
Metagenoma
Filogenia
Prevalência
Sus scrofa
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Capsid Proteins); EC 3.4.- (Cysteine Proteases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190819


  2 / 137 MEDLINE  
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[PMID]:28590234
[Au] Autor:Knutson TP; Velayudhan BT; Marthaler DG
[Ad] Endereço:1​Veterinary Diagnostic Laboratory, Department of Veterinary Population Medicine, University of Minnesota, St Paul, MN 55108, USA.
[Ti] Título:A porcine enterovirus G associated with enteric disease contains a novel papain-like cysteine protease.
[So] Source:J Gen Virol;98(6):1305-1310, 2017 Jun.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Identification of unknown pathogens in pigs displaying enteric illness is difficult due to the large diversity of bacterial and viral species found within faecal samples. Current methods often require bacterial or viral isolation, or testing only a limited number of known species using quantitative PCR analysis. Herein, faeces from two 25-day-old piglets with diarrhoea from Texas, USA, were analysed by metagenomic next-generation sequencing to rapidly identify possible pathogens. Our analysis included a bioinformatics pipeline of rapid short-read classification and de novo genome assembly which resulted in the identification of a porcine enterovirus G (EV-G), a complete genome with substantial nucleotide differences (>30 %) among current sequences, and a novel non-structural protein similar in sequence to the Torovirus papain-like cysteine protease (PLpro). This discovery led to the identification and circulation of an EV-G with a novel PLpro in the USA that has not been previously reported.
[Mh] Termos MeSH primário: Cisteína Proteases/genética
Diarreia/veterinária
Infecções por Enterovirus/veterinária
Enterovirus Suínos/classificação
Enterovirus Suínos/enzimologia
Fezes/virologia
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Animais
Análise por Conglomerados
Biologia Computacional
Diarreia/virologia
Infecções por Enterovirus/virologia
Enterovirus Suínos/genética
Enterovirus Suínos/isolamento & purificação
Genoma Viral
Sequenciamento de Nucleotídeos em Larga Escala
Filogenia
Análise de Sequência de DNA
Suínos
Texas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.4.- (Cysteine Proteases)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171115
[Lr] Data última revisão:
171115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000799


  3 / 137 MEDLINE  
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[PMID]:28555547
[Au] Autor:Lukashev AN; Corman VM; Schacht D; Gloza-Rausch F; Seebens-Hoyer A; Gmyl AP; Drosten C; Drexler JF
[Ad] Endereço:1​Chumakov Institute of Poliomyelitis and Viral Encephalitides, Moscow, Russia 2​Institute of Molecular Medicine, Sechenov First Moscow State Medical University, Moscow, Russia.
[Ti] Título:Close genetic relatedness of picornaviruses from European and Asian bats.
[So] Source:J Gen Virol;98(5):955-961, 2017 May.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Our investigation of 1004 faecal specimens from European bats for picornaviruses by broadly reactive nested reverse transcription-PCR found picornaviral RNA in 28 samples (2.8 %). Phylogenetic analysis of the partial 3D genomic region suggested that one bat virus belonged to the species Enterovirus G (EV-G, formerly Porcine enterovirus B). Bat infection was supported by relatively high EV-G concentrations of 1.1×106 RNA copies per gram of faeces. All other bat viruses belonged either to the bat-associated genus Mischivirus, or to an unclassified Picornaviridae group distantly related to the genus Sapelovirus. Members of this unclassified sapelovirus-related group had RNA secondary structures in their 3'-nontranslated regions that were typical of enteroviruses and that resembled structures that occur in bat-associated coronaviruses, suggesting ancient recombination events. Based on sequence distances, several picornaviruses from European and Chinese bats were likely conspecific, suggesting connectivity of virus populations. Due to their high mutation rates and their diversity, picornaviruses may be useful tools for studies of bat and virus ecology.
[Mh] Termos MeSH primário: Quirópteros/virologia
Picornaviridae/classificação
Picornaviridae/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Ásia
Análise por Conglomerados
Enterovirus Suínos
Europa (Continente)
Fezes/virologia
Genoma Viral
Filogenia
Picornaviridae/genética
Reação em Cadeia da Polimerase
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Análise de Sequência de DNA
Homologia de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170531
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000760


  4 / 137 MEDLINE  
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[PMID]:28160432
[Au] Autor:Arruda PH; Arruda BL; Schwartz KJ; Vannucci F; Resende T; Rovira A; Sundberg P; Nietfeld J; Hause BM
[Ad] Endereço:Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
[Ti] Título:Detection of a novel sapelovirus in central nervous tissue of pigs with polioencephalomyelitis in the USA.
[So] Source:Transbound Emerg Dis;64(2):311-315, 2017 Apr.
[Is] ISSN:1865-1682
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:An approximately 3,000 finishing swine operation in the United States experienced an outbreak of an atypical neurologic disease in 11-weeks-old pigs with an overall morbidity of 20% and case fatality rate of 30%. The clinical onset and progression of signs in affected pigs varied but included inappetence, compromised ambulation, ataxia, incoordination, mental dullness, paresis, paralysis and decreased response to environmental stimuli. Tissues from affected pigs were submitted for diagnostic investigation. Histopathologic examination of the cerebrum, cerebellum and spinal cord revealed severe lymphoplasmacytic and necrotizing polioencephalomyelitis with multifocal areas of gliosis and neuron satellitosis, suggestive of a neurotropic viral infection. Bacterial pathogens were not isolated by culture of neurologic tissue from affected pigs. Samples tested by polymerase chain reaction (PCR) were negative for pseudorabies virus and atypical porcine pestivirus. Immunohistochemistry for porcine reproductive and respiratory syndrome virus, porcine circovirus and Listeria was negative. Porcine sapelovirus (PSV) was identified in spinal cord by a nested PCR used to detect porcine enterovirus, porcine teschovirus and PSV. Next-generation sequencing of brainstem and spinal cord samples identified PSV and the absence of other or novel pathogens. In addition, Sapelovirus A mRNA was detected in neurons and nerve roots of the spinal cord by in situ hybridization. The PSV is genetically novel with an overall 94% amino acid identity and 86% nucleotide identity to a recently reported sapelovirus from Korea. This is the first case report in the United States associating sapelovirus with severe polioencephalomyelitis in pigs.
[Mh] Termos MeSH primário: Infecções por Circoviridae/epidemiologia
Encefalomielite Enzoótica Suína/virologia
Infecções por Enterovirus/veterinária
Infecções por Picornaviridae/veterinária
Picornaviridae/isolamento & purificação
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Animais
Surtos de Doenças
Infecções por Enterovirus/virologia
Enterovirus Suínos/isolamento & purificação
Imuno-Histoquímica
Hibridização In Situ
Tecido Nervoso/virologia
Picornaviridae/genética
Reação em Cadeia da Polimerase
Vírus de RNA
Suínos
Doenças dos Suínos/epidemiologia
Estados Unidos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170620
[Lr] Data última revisão:
170620
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170205
[St] Status:MEDLINE
[do] DOI:10.1111/tbed.12621


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[PMID]:27707918
[Au] Autor:Zhu Z; Wang G; Yang F; Cao W; Mao R; Du X; Zhang X; Li C; Li D; Zhang K; Shu H; Liu X; Zheng H
[Ad] Endereço:State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
[Ti] Título:Foot-and-Mouth Disease Virus Viroporin 2B Antagonizes RIG-I-Mediated Antiviral Effects by Inhibition of Its Protein Expression.
[So] Source:J Virol;90(24):11106-11121, 2016 Dec 15.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The role of retinoic acid-inducible gene I (RIG-I) in foot-and-mouth disease virus (FMDV)-infected cells remains unknown. Here, we showed that RIG-I inhibits FMDV replication in host cells. FMDV infection increased the transcription of RIG-I, while it decreased RIG-I protein expression. A detailed analysis revealed that FMDV leader proteinase (L ), as well as 3C proteinase (3C ) and 2B protein, decreased RIG-I protein expression. L and 3C are viral proteinases that can cleave various host proteins and are responsible for several of the viral polyprotein cleavages. However, for the first time, we observed 2B-induced reduction of host protein. Further studies showed that 2B-mediated reduction of RIG-I is specific to FMDV, but not other picornaviruses, including encephalomyocarditis virus, enterovirus 71, and coxsackievirus A16. Moreover, we found the decreased protein level of RIG-I is independent of the cleavage of eukaryotic translation initiation factor 4 gamma, the induction of cellular apoptosis, or the association of proteasome, lysosome, and caspase pathways. A direct interaction was observed between RIG-I and 2B. The carboxyl-terminal amino acids 105 to 114 and amino acids 135 to 144 of 2B were essential for the reduction of RIG-I, while residues 105 to 114 were required for the interaction. These data suggest the antiviral role of RIG-I against FMDV and a novel antagonistic mechanism of FMDV that is mediated by 2B protein. IMPORTANCE: This study demonstrated that RIG-I could suppress FMDV replication during virus infection. FMDV infection increased the transcriptional expression of RIG-I, while it decreased RIG-I protein expression. FMDV 2B protein interacted with RIG-I and induced reduction of RIG-I. 2B-induced reduction of RIG-I was independent of the induction of the cleavage of eukaryotic translation initiation factor 4 gamma or cellular apoptosis. In addition, proteasome, lysosome, and caspase pathways were not involved in this process. This study provides new insight into the immune evasion mediated by FMDV and identifies 2B as an antagonistic factor for FMDV to evade the antiviral response.
[Mh] Termos MeSH primário: Cisteína Endopeptidases/genética
Proteína DEAD-box 58/genética
Endopeptidases/genética
Fator de Iniciação 4G em Eucariotos/genética
Vírus da Febre Aftosa/genética
Interações Hospedeiro-Patógeno
Proteínas Virais/genética
Proteínas Virais Reguladoras e Acessórias/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sistemas CRISPR-Cas
Linhagem Celular
Cricetulus
Cisteína Endopeptidases/imunologia
Proteína DEAD-box 58/imunologia
Vírus da Encefalomiocardite/genética
Vírus da Encefalomiocardite/imunologia
Endopeptidases/imunologia
Enterovirus/genética
Enterovirus/imunologia
Enterovirus Suínos/genética
Enterovirus Suínos/imunologia
Células Epiteliais
Fator de Iniciação 4G em Eucariotos/imunologia
Vírus da Febre Aftosa/imunologia
Regulação da Expressão Gênica
Técnicas de Silenciamento de Genes
Células HEK293
Seres Humanos
Ligação Proteica
Transdução de Sinais
Especificidade da Espécie
Suínos
Proteínas Virais/imunologia
Proteínas Virais Reguladoras e Acessórias/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Eukaryotic Initiation Factor-4G); 0 (Viral Proteins); 0 (Viral Regulatory and Accessory Proteins); EC 3.4.- (Endopeptidases); EC 3.4.22.- (Cysteine Endopeptidases); EC 3.4.22.28 (3C proteases); EC 3.4.99.- (leader proteinase, foot-and-mouth disease virus); EC 3.6.4.13 (DEAD Box Protein 58)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161007
[St] Status:MEDLINE


  6 / 137 MEDLINE  
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[PMID]:26865725
[Au] Autor:Kim DS; Son KY; Koo KM; Kim JY; Alfajaro MM; Park JG; Hosmillo M; Soliman M; Baek YB; Cho EH; Lee JH; Kang MI; Goodfellow I; Cho KO
[Ad] Endereço:Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
[Ti] Título:Porcine Sapelovirus Uses α2,3-Linked Sialic Acid on GD1a Ganglioside as a Receptor.
[So] Source:J Virol;90(8):4067-77, 2016 Apr.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: The receptor(s) for porcine sapelovirus (PSV), which causes diarrhea, pneumonia, polioencephalomyelitis, and reproductive disorders in pigs, remains largely unknown. Given the precedent for other picornaviruses which use terminal sialic acids (SAs) as receptors, we examined the role of SAs in PSV binding and infection. Using a variety of approaches, including treating cells with a carbohydrate-destroying chemical (NaIO4), mono- or oligosaccharides (N-acetylneuraminic acid, galactose, and 6'-sialyllactose), linkage-specific sialidases (neuraminidase and sialidase S), lectins (Maakia amurensislectin andSambucus nigralectin), proteases (trypsin and chymotrypsin), and glucosylceramide synthase inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol and phospholipase C), we demonstrated that PSV could recognize α2,3-linked SA on glycolipids as a receptor. On the other hand, PSVs had no binding affinity for synthetic histo-blood group antigens (HBGAs), suggesting that PSVs could not use HBGAs as receptors. Depletion of cell surface glycolipids followed by reconstitution studies indicated that GD1a ganglioside, but not other gangliosides, could restore PSV binding and infection, further confirming α2,3-linked SA on GD1a as a PSV receptor. Our results could provide significant information on the understanding of the life cycle of sapelovirus and other picornaviruses. For the broader community in the area of pathogens and pathogenesis, these findings and insights could contribute to the development of affordable, useful, and efficient drugs for anti-sapelovirus therapy. IMPORTANCE: The porcine sapelovirus (PSV) is known to cause enteritis, pneumonia, polioencephalomyelitis, and reproductive disorders in pigs. However, the receptor(s) that the PSV utilizes to enter host cells remains largely unknown. Using a variety of approaches, we showed that α2,3-linked terminal sialic acid (SA) on the cell surface GD1a ganglioside could be used for PSV binding and infection as a receptor. On the other hand, histo-blood group antigens also present in the cell surface carbohydrates could not be utilized as PSV receptors for binding and infection. These findings should contribute to the understanding of the sapelovirus life cycle and to the development of affordable, useful and efficient drugs for anti-sapelovirus therapy.
[Mh] Termos MeSH primário: Enterovirus Suínos/metabolismo
Gangliosídeos/metabolismo
Ácido N-Acetilneuramínico/metabolismo
Receptores Virais/metabolismo
[Mh] Termos MeSH secundário: Animais
Antígenos de Grupos Sanguíneos/metabolismo
Carboidratos/química
Linhagem Celular
Células HeLa
Seres Humanos
Ácido N-Acetilneuramínico/química
Receptores Virais/química
Suínos
Ligação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Blood Group Antigens); 0 (Carbohydrates); 0 (Gangliosides); 0 (Receptors, Virus); 12707-58-3 (ganglioside, GD1a); GZP2782OP0 (N-Acetylneuraminic Acid)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160212
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.02449-15


  7 / 137 MEDLINE  
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[PMID]:26711031
[Au] Autor:Zhou W; Ullman K; Chowdry V; Reining M; Benyeda Z; Baule C; Juremalm M; Wallgren P; Schwarz L; Zhou E; Pedrero SP; Hennig-Pauka I; Segales J; Liu L
[Ad] Endereço:College of Veterinary Medicine, Inner Mongolia Agricultural University, Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture, Hohhot, China; National Veterinary Institute (SVA), Uppsala, Sweden.
[Ti] Título:Molecular investigations on the prevalence and viral load of enteric viruses in pigs from five European countries.
[So] Source:Vet Microbiol;182:75-81, 2016.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Enteric viral infections in pigs may cause diarrhea resulting in ill-thrift and substantial economic losses. This study reports the enteric infections with porcine astrovirus type 4 (PAstV4), porcine group A rotavirus (GARV), porcine group C rotavirus (GCRV), porcine circovirus type 2 (PCV2) and porcine kobuvirus (PKoV) in 419 pigs, comprising both healthy and diarrheic animals, from 49 farms in five European countries (Austria, Germany, Hungary, Spain and Sweden). Real-time RT-PCR assays were developed to test fecal samples and to compare the prevalence and viral load in relation to health status, farms of origin and age groups. The results showed that PAstV4 (70.4%) was the dominant virus species, followed by PKoV (56.7%), PCV2 (42.2%), GCRV (3%) and GARV (0.9%). Diarrheic pigs had a higher viral load of PAstV4 in the nursery and growing-finishing groups. Rotaviruses were mainly detected in diarrheic pigs, whereas PCV2 was more often detected in clinically healthy than in diarrheic pigs, suggesting that most PCV2 infections were subclinical. PAstV4, PCV2 and PKoV were considered ubiquitous in the European pig livestock and co-infections among them were frequent, independently of the disease status, in contrast to a low prevalence of classical rotavirus infections.
[Mh] Termos MeSH primário: Enterovirus Suínos/isolamento & purificação
Doenças dos Suínos/virologia
Carga Viral
[Mh] Termos MeSH secundário: Animais
Europa (Continente)/epidemiologia
Prevalência
RNA Viral/genética
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária
Suínos
Doenças dos Suínos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:151229
[Lr] Data última revisão:
151229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151230
[St] Status:MEDLINE


  8 / 137 MEDLINE  
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[PMID]:25199345
[Au] Autor:Derev'ianko SV
[Ti] Título:[New classification of enteroviruses of pigs: serological and molecular genetic aspects].
[So] Source:Mikrobiol Z;76(4):47-53, 2014 Jul-Aug.
[Is] ISSN:1028-0987
[Cp] País de publicação:Ukraine
[La] Idioma:ukr
[Ab] Resumo:Due to changes in modern classification and nomenclature family Picornaviridae made by the International Committee of Taxonomy of Viruses, conducted genetic and serological reclassification of 36 industrial, typical and laboratory strains porcine enteroviruses isolated in Ukraine. The results of molecular genetic studies of 34 strains assigned to the family Picornaviridae, genus Teschovirus, species Porcine teschovirus, 2 strains of virus did not engage in polymerase chain reaction with species specific primer. In the neutralization reaction of the virus revealed that 23 strains belonging to 1 serotype Porcine teschovirus, 4 strain--PTV-3, 1--to PTV-6, 1 strain--to Porcine sapelovirus, three strains have between typical antigenic properties, and 4 strains--antigenically different from reference strains Porcine teschovirus, Enterovirus G and Porcine sapelovirus.
[Mh] Termos MeSH primário: DNA Viral
Enterovirus Suínos/classificação
Enterovirus Suínos/ultraestrutura
Suínos/virologia
[Mh] Termos MeSH secundário: Animais
Classificação
DNA Viral/genética
Enterovirus Suínos/genética
Enterovirus Suínos/isolamento & purificação
Microscopia Eletrônica
Reação em Cadeia da Polimerase
Sorotipagem
Suínos/embriologia
Ucrânia
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:140909
[Lr] Data última revisão:
140909
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140910
[St] Status:MEDLINE


  9 / 137 MEDLINE  
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[PMID]:24824640
[Au] Autor:Anbalagan S; Hesse RA; Hause BM
[Ad] Endereço:Newport Laboratories, Inc., Worthington, Minnesota, United States of America.
[Ti] Título:First identification and characterization of porcine enterovirus G in the United States.
[So] Source:PLoS One;9(5):e97517, 2014.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Porcine enterovirus G (EV-G) is a member of the family Picornavirdae, genus Enterovirus. To date, eleven EV-G types (EV-G1 through EV-G11) have been identified in pigs from Asia and Europe however they have never been reported in North America. In this study, we isolated and characterized the complete genome of NP/2013/USA, an EV-G from a porcine diarrhea sample from the United States. The complete genome consists of 7,390 nucleotides excluding the 3' poly(A) tail, and has an open reading frame that encodes a 2,169 amino acid polyprotein. NP/2013/USA was most similar at the nucleotide (84%) and amino acid (95%) level to the HM131607, an EV-G1 type isolated from China in 2012.
[Mh] Termos MeSH primário: Enterovirus Suínos/genética
Genoma Viral/genética
Filogenia
Suínos/virologia
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Primers do DNA/genética
Enterovirus Suínos/classificação
Fezes/virologia
Funções Verossimilhança
Modelos Genéticos
Dados de Sequência Molecular
Fases de Leitura Aberta/genética
Análise de Sequência de DNA
Homologia de Sequência
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA Primers)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:150805
[Lr] Data última revisão:
150805
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140515
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0097517


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[PMID]:24323635
[Au] Autor:Van Dung N; Anh PH; Van Cuong N; Hoa NT; Carrique-Mas J; Hien VB; Campbell J; Baker S; Farrar J; Woolhouse ME; Bryant JE; Simmonds P
[Ad] Endereço:Infection and Immunity Division, Roslin Institute, University of Edinburgh, Easter Bush, Edinburgh EH25 9RG, UK.
[Ti] Título:Prevalence, genetic diversity and recombination of species G enteroviruses infecting pigs in Vietnam.
[So] Source:J Gen Virol;95(Pt 3):549-56, 2014 Mar.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Picornaviruses infecting pigs, described for many years as 'porcine enteroviruses', have recently been recognized as distinct viruses within three distinct genera (Teschovirus, Sapelovirus and Enterovirus). To better characterize the epidemiology and genetic diversity of members of the Enterovirus genus, faecal samples from pigs from four provinces in Vietnam were screened by PCR using conserved enterovirus (EV)-specific primers from the 5' untranslated region (5' UTR). High rates of infection were recorded in pigs on all farms, with detection frequencies of approximately 90% in recently weaned pigs but declining to 40% in those aged over 1 year. No differences in EV detection rates were observed between pigs with and without diarrhoea [74% (n = 70) compared with 72% (n = 128)]. Genetic analysis of consensus VP4/VP2 and VP1 sequences amplified from a subset of EV-infected pigs identified species G EVs in all samples. Among these, VP1 sequence comparisons identified six type 1 and seven type 6 variants, while four further VP1 sequences failed to group with any previously identified EV-G types. These have now been formally assigned as EV-G types 8-11 by the Picornavirus Study Group. Comparison of VP1, VP4/VP2, 3D(pol) and 5' UTRs of study samples and those available on public databases showed frequent, bootstrap-supported differences in their phylogenies indicative of extensive within-species recombination between genome regions. In summary, we identified extremely high frequencies of infection with EV-G in pigs in Vietnam, substantial genetic diversity and recombination within the species, and evidence for a much larger number of circulating EV-G types than currently described.
[Mh] Termos MeSH primário: Infecções por Enterovirus/veterinária
Enterovirus Suínos/genética
Variação Genética
Recombinação Genética
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Animais
Infecções por Enterovirus/epidemiologia
Infecções por Enterovirus/virologia
Enterovirus Suínos/classificação
Enterovirus Suínos/isolamento & purificação
Dados de Sequência Molecular
Filogenia
Prevalência
Sus scrofa
Suínos
Doenças dos Suínos/epidemiologia
Vietnã/epidemiologia
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1405
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131211
[St] Status:MEDLINE
[do] DOI:10.1099/vir.0.061978-0



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