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[PMID]: 29262797 [Au] Autor: Jiang W; Wu M; Zhou J; Wang Y; Hao C; Ji W; Zhang X; Gu W; Shao X [Ad] Endereço: Department of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou, China. [Ti] Título: Etiologic spectrum and occurrence of coinfections in children hospitalized with community-acquired pneumonia. [So] Source: BMC Infect Dis;17(1):787, 2017 Dec 20. [Is] ISSN: 1471-2334 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Co-infections are common in childhood community acquired pneumonia (CAP). However, their etiological pattern and clinical impact remains inconclusive. METHODS: Eight hundred forty-six consecutive children with CAP were evaluated prospectively for the presence of viral and bacterial pathogens. Nasopharyngeal aspirates were examined by direct immunofluorescence assay or polymerase chain reaction (PCR) for viruses. PCR of nasopharyngeal aspirates and enzyme-linked immunosorbent assays were performed to detect M. pneumoniae. Bacteria was detected in blood, bronchoalveolar lavage specimen, or pleural fluid by culture. RESULTS: Causative pathogen was identified in 70.1% (593 of 846) of the patients. The most commonly detected pathogens were respiratory syncytial virus (RSV) (22.9%), human rhinovirus (HRV) (22.1%), M. pneumoniae (15.8%). Coinfection was identified in 34.6% (293 of 846) of the patients. The majority of these (209 [71.3%] of 293) were mixed viral-bacterial infections. Age < 6 months (odds ratio: 2.1; 95% confidence interval: 1.2-3.3) and admission of PICU (odds ratio: 12.5; 95% confidence interval: 1.6-97.4) were associated with mix infection. Patients with mix infection had a higher rate of PICU admission. CONCLUSIONS: The high mix infection burden in childhood CAP underscores a need for the enhancement of sensitive, inexpensive, and rapid diagnostics to accurately identify pneumonia pathogens. [Mh] Termos MeSH primário: Coinfecção Infecções Comunitárias Adquiridas Pneumonia [Mh] Termos MeSH secundário: Adolescente Criança Pré-Escolar China/epidemiologia Coinfecção/epidemiologia Coinfecção/microbiologia Coinfecção/virologia Infecções Comunitárias Adquiridas/epidemiologia Infecções Comunitárias Adquiridas/microbiologia Infecções Comunitárias Adquiridas/virologia Feminino Seres Humanos Lactente Masculino Mycoplasma pneumoniae Pneumonia/epidemiologia Pneumonia/microbiologia Pneumonia/virologia Vírus Sincicial Respiratório Humano Rhinovirus [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180309 [Lr] Data última revisão: 180309 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171222 [St] Status: MEDLINE [do] DOI: 10.1186/s12879-017-2891-x

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[PMID]: 28452706 [Au] Autor: Rowan NR; Wang EW; Kanaan A; Sahu N; Williams JV; Phillips CD; Lee SE [Ad] Endereço: Department of Otolaryngology-Head and Neck Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA. [Ti] Título: Respiratory viral detection in the paranasal sinuses of patients with cystic fibrosis. [So] Source: Am J Rhinol Allergy;31(2):105-108, 2017 Mar 01. [Is] ISSN: 1945-8932 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: BACKGROUND: Pulmonary colonization with antibiotic-resistant organisms in patients with cystic fibrosis (CF) is often preceded by upper-airway infections. Although there is a well-described relationship between pulmonary respiratory viral infections and overall disease progression of CF, the pathogenicity of respiratory viral infections in the paranasal sinuses of patients with CF remains unknown. With recent advances in respiratory virus detection techniques, this study sought to detect the presence of respiratory viruses in the paranasal sinuses of patients with CF in comparison with healthy controls and to correlate the viral presence with clinical measures of sinonasal disease. METHODS: This prospective individual cohort study compared 24 patients with CF with 14 healthy controls. Basic demographics, clinical measures of disease and respiratory viral screens (commercial multiplex) obtained directly from the paranasal sinuses were compared between the two groups. RESULTS: Respiratory viruses were detected in 33% of patients with CF (8/24) compared with 0% of the healthy controls (0/14) (p = 0.017). Respiratory viruses were only detected during the winter months, and the most commonly identified were influenza A and human rhinovirus strains. There was no statistical difference in the 22-Item Sino-Nasal Outcome Test (SNOT-22) scores (p = 0.93) or modified Lund-Kennedy scores (p = 0.74) between patients with CF with a positive viral test and those without a positive result. CONCLUSIONS: Respiratory viral detection is more commonly detected in the paranasal sinuses of patients with CF compared with healthy controls. Although respiratory viral presence did not correlate with a worse clinical severity of sinonasal disease, these findings may provide insight into the pathophysiology of CF and open new avenues for potential targeted therapy. [Mh] Termos MeSH primário: Fibrose Cística/epidemiologia Fibrose Cística/virologia Vírus da Influenza A/fisiologia Influenza Humana/epidemiologia Seios Paranasais/virologia Infecções por Picornaviridae/epidemiologia Rhinovirus/fisiologia [Mh] Termos MeSH secundário: Adulto Estudos de Coortes Progressão da Doença Feminino Seres Humanos Masculino Meia-Idade Estudos Prospectivos Estações do Ano [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180309 [Lr] Data última revisão: 180309 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170429 [St] Status: MEDLINE [do] DOI: 10.2500/ajra.2017.31.4422

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[PMID]: 28459437 [Au] Autor: Toussaint M; Jackson DJ; Swieboda D; Guedán A; Tsourouktsoglou TD; Ching YM; Radermecker C; Makrinioti H; Aniscenko J; Bartlett NW; Edwards MR; Solari R; Farnir F; Papayannopoulos V; Bureau F; Marichal T; Johnston SL [Ad] Endereço: Airway Disease Infection Section, National Heart and Lung Institute (NHLI), Imperial College London, London, UK. [Ti] Título: Host DNA released by NETosis promotes rhinovirus-induced type-2 allergic asthma exacerbation. [So] Source: Nat Med;23(6):681-691, 2017 06. [Is] ISSN: 1546-170X [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Respiratory viral infections represent the most common cause of allergic asthma exacerbations. Amplification of the type-2 immune response is strongly implicated in asthma exacerbation, but how virus infection boosts type-2 responses is poorly understood. We report a significant correlation between the release of host double-stranded DNA (dsDNA) following rhinovirus infection and the exacerbation of type-2 allergic inflammation in humans. In a mouse model of allergic airway hypersensitivity, we show that rhinovirus infection triggers dsDNA release associated with the formation of neutrophil extracellular traps (NETs), known as NETosis. We further demonstrate that inhibiting NETosis by blocking neutrophil elastase or by degrading NETs with DNase protects mice from type-2 immunopathology. Furthermore, the injection of mouse genomic DNA alone is sufficient to recapitulate many features of rhinovirus-induced type-2 immune responses and asthma pathology. Thus, NETosis and its associated extracellular dsDNA contribute to the pathogenesis and may represent potential therapeutic targets of rhinovirus-induced asthma exacerbations. [Mh] Termos MeSH primário: Asma/imunologia Citocinas/imunologia DNA/imunologia Armadilhas Extracelulares/imunologia Infecções por Picornaviridae/imunologia Hipersensibilidade Respiratória/imunologia Infecções Respiratórias/imunologia Células Th2/imunologia [Mh] Termos MeSH secundário: Adulto Animais Estudos de Casos e Controles Dermatophagoides farinae/imunologia Modelos Animais de Doenças Feminino Seres Humanos Interferon gama/imunologia Interleucina-13/imunologia Interleucina-4/imunologia Interleucina-5/imunologia Masculino Camundongos Meia-Idade Rhinovirus Adulto Jovem [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Cytokines); 0 (Interleukin-13); 0 (Interleukin-5); 207137-56-2 (Interleukin-4); 82115-62-6 (Interferon-gamma); 9007-49-2 (DNA) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 180301 [Lr] Data última revisão: 180301 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170502 [St] Status: MEDLINE [do] DOI: 10.1038/nm.4332

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[PMID]: 28467361 [Au] Autor: Francioso A; Cossi R; Fanelli S; Mastromarino P; Mosca L [Ad] Endereço: Department of Biochemical Sciences, "Sapienza" University of Rome, Piazzale Aldo Moro, 5, 00185 Roma, Italy. antonio.francioso@uniroma1.it. [Ti] Título: Studies on Trans-Resveratrol/Carboxymethylated (1,3/1,6)-ß-d-Glucan Association for Aerosol Pharmaceutical Applications. [So] Source: Int J Mol Sci;18(5), 2017 May 03. [Is] ISSN: 1422-0067 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: A resveratrol/carboxymethylated glucan (CM-glucan) combination is known to inhibit rhinovirus replication and expression of inflammatory mediators in nasal epithelia. The aim of this study was to develop an aerosol formulation containing an association of the two molecules which could reach the lower respiratory tract. Mass median aerodynamic diameter (MMAD) of a resveratrol/CM-glucan combination was lower than that shown by resveratrol or CM-glucan alone (2.83 versus 3.28 and 2.96 µm, respectively). The resveratrol/CM-glucan association results in the finest and most monodispersed particles in comparison to the two single components. The association also evidenced lower values for all particle size distribution parameters, suggesting that the pharmacological synergy observed in previous studies may be accompanied by a pharmaceutical one. Moreover, we showed that the CM-glucan matrix did not exert an inhibitory effect on resveratrol nebulization, demonstrating the good suitability of these two molecules in association for simultaneous aerosol volatilization. [Mh] Termos MeSH primário: Anti-Inflamatórios não Esteroides/química Anti-Inflamatórios não Esteroides/farmacologia Antivirais/química Antivirais/farmacologia Estilbenos/química Estilbenos/farmacologia beta-Glucanas/química [Mh] Termos MeSH secundário: Aerossóis Sobrevivência Celular/efeitos dos fármacos Células HeLa Seres Humanos Mucosa Nasal Nebulizadores e Vaporizadores Tamanho da Partícula Rhinovirus/efeitos dos fármacos Volatilização [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Aerosols); 0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Antiviral Agents); 0 (Stilbenes); 0 (beta-Glucans); 9051-97-2 (beta-1,3-glucan); Q369O8926L (resveratrol) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180221 [Lr] Data última revisão: 180221 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170504 [St] Status: MEDLINE

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[PMID]: 28966037 [Au] Autor: Allmaier G; Blaas D; Bliem C; Dechat T; Fedosyuk S; Gösler I; Kowalski H; Weiss VU [Ad] Endereço: Institute of Chemical Technologies and Analytics, TU Wien (Vienna University of Technology), Vienna, Austria. [Ti] Título: Monolithic anion-exchange chromatography yields rhinovirus of high purity. [So] Source: J Virol Methods;251:15-21, 2018 Jan. [Is] ISSN: 1879-0984 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: For vaccine development, 3D-structure determination, direct fluorescent labelling, and numerous other studies, homogeneous virus preparations of high purity are essential. Working with human rhinoviruses (RVs), members of the picornavirus family and the main cause of generally mild respiratory infections, we noticed that our routine preparations appeared highly pure on analysis by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), exclusively showing the four viral capsid proteins (VPs). However, the preparations turned out to contain substantial amounts of contaminating material when analyzed by orthogonal analytical methods including capillary zone electrophoresis, nano electrospray gas-phase electrophoretic mobility molecular analysis (nES GEMMA), and negative stain transmission electron microscopy (TEM). Because these latter analyses are not routine to many laboratories, the above contaminations might remain unnoticed and skew experimental results. By using human rhinovirus serotype A2 (RV-A2) as example we report monolithic anion-exchange chromatography (AEX) as a last polishing step in the purification and demonstrate that it yields infective, highly pure, virus (RV-A2 in the respective fractions was confirmed by peptide mass fingerprinting) devoid of foreign material as judged by the above criteria. [Mh] Termos MeSH primário: Cromatografia por Troca Iônica/métodos Rhinovirus/isolamento & purificação Virologia/métodos [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180201 [Lr] Data última revisão: 180201 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171003 [St] Status: MEDLINE

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[PMID]: 28471477 [Au] Autor: Ross C; Knox C; Tastan Bishop Ö [Ad] Endereço: Research Unit in Bioinformatics (RUBi), Department of Biochemistry and Microbiology, Rhodes University, Grahamstown, South Africa. [Ti] Título: Interacting motif networks located in hotspots associated with RNA release are conserved in Enterovirus capsids. [So] Source: FEBS Lett;591(12):1687-1701, 2017 06. [Is] ISSN: 1873-3468 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Enteroviruses are responsible for a multitude of human diseases. Expansion of the virus capsid is associated with a cascade of conformational changes that allow the subsequent release of RNA. For the first time, this study presents a comprehensive bioinformatic screen for the prediction of interacting motifs within intraprotomer interfaces and across respective interfaces surrounding the fivefold and twofold axes. The results identify a network of conserved motif residues involved in interactions in enteroviruses that may be critical to capsid stabilisation, providing guidelines towards developing antivirals that interfere with viral expansion during RNA release. [Mh] Termos MeSH primário: Proteínas do Capsídeo/metabolismo Enterovirus/metabolismo Modelos Moleculares RNA Viral/metabolismo Rhinovirus/metabolismo [Mh] Termos MeSH secundário: Motivos de Aminoácidos Sequência de Aminoácidos Substituição de Aminoácidos Proteínas do Capsídeo/química Proteínas do Capsídeo/genética Biologia Computacional Sequência Conservada Cristalografia por Raios X Bases de Dados de Proteínas Transferência de Energia Mutação Domínios e Motivos de Interação entre Proteínas Multimerização Proteica Estabilidade Proteica Subunidades Proteicas/química Subunidades Proteicas/genética Subunidades Proteicas/metabolismo RNA Viral/química Especificidade da Espécie Propriedades de Superfície [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Capsid Proteins); 0 (Protein Subunits); 0 (RNA, Viral) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 180130 [Lr] Data última revisão: 180130 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170505 [St] Status: MEDLINE [do] DOI: 10.1002/1873-3468.12663

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[PMID]: 29246347 [Au] Autor: Droz N; Enouf V; Bidet P; Mohamed D; Behillil S; Simon AL; Bachy M; Caseris M; Bonacorsi S; Basmaci R [Ad] Endereço: Pediatric-Emergency Department, Louis-Mourier Hospital, AP-HP, Colombes, France. [Ti] Título: Temporal Association Between Rhinovirus Activity and Kingella kingae Osteoarticular Infections. [So] Source: J Pediatr;192:234-239.e2, 2018 Jan. [Is] ISSN: 1097-6833 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: OBJECTIVE: To determine whether the seasonal distribution of Kingella kingae osteoarticular infections is similar to that of common respiratory viruses. STUDY DESIGN: Between October 2009 and September 2016, we extracted the results of K kingae-specific real-time polymerase chain reaction analyses performed for bone or joint specimens in patients from 2 pediatric tertiary care centers in Paris. We used data of respiratory virus detection from the Réseau National des Laboratoires network with coordination with the National Influenza Center of France. The Spearman rank correlation was used to assess a correlation between weekly distributions, with P < .05 denoting a significant correlation. RESULTS: During the 7-year study period, 322 children were diagnosed with K kingae osteoarticular infection, and 317 testing episodes were K kingae-negative. We observed high activity for both K kingae osteoarticular infection and human rhinovirus (HRV) during the fall (98 [30.4%] and 2401 [39.1%] cases, respectively) and low activity during summer (59 [18.3%] and 681 [11.1%] cases, respectively). Weekly distributions of K kingae osteoarticular infection and rhinovirus activity were significantly correlated (r = 0.30; P = .03). In contrast, no significant correlation was found between the weekly distribution of K kingae osteoarticular infection and other respiratory viruses (r = -0.17, P = .34 compared with respiratory syncytial virus; r = -0.13, P = .34 compared with influenza virus; and r = -0.22, P = .11 compared with metapneumovirus). CONCLUSION: A significant temporal association was observed between HRV circulation and K kingae osteoarticular infection, strengthening the hypothesis of a role of viral infections in the pathophysiology of K kingae invasive infection. [Mh] Termos MeSH primário: Artrite Infecciosa/epidemiologia Kingella kingae Infecções por Neisseriaceae/epidemiologia Infecções por Picornaviridae/epidemiologia Rhinovirus Estações do Ano [Mh] Termos MeSH secundário: Artrite Infecciosa/diagnóstico Artrite Infecciosa/virologia Pré-Escolar França/epidemiologia Seres Humanos Lactente Kingella kingae/isolamento & purificação Infecções por Neisseriaceae/diagnóstico Infecções por Neisseriaceae/virologia Reação em Cadeia da Polimerase em Tempo Real [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1712 [Cu] Atualização por classe: 171226 [Lr] Data última revisão: 171226 [Sb] Subgrupo de revista: AIM; IM [Da] Data de entrada para processamento: 171217 [St] Status: MEDLINE

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[PMID]: 29182620 [Au] Autor: Roth M; Pasquali C; Stolz D; Tamm M [Ad] Endereço: Pulmonary Cell Research, DBM University Basel and Pneumology Clinic, University Hospital Basel, Basel, Switzerland. [Ti] Título: Broncho Vaxom (OM-85) modulates rhinovirus docking proteins on human airway epithelial cells via Erk1/2 mitogen activated protein kinase and cAMP. [So] Source: PLoS One;12(11):e0188010, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: BACKGROUND: Bronchial epithelial cells (BEC) are primary target for Rhinovirus infection through attaching to cell membrane proteins. OM-85, a bacterial extract, improves recovery of asthma and COPD patients after viral infections, but only part of the mechanism was addressed, by focusing on defined immune cells. OBJECTIVE: We therefore determined the effect of OM-85 on isolated primary human BEC of controls (n = 8), asthma patients (n = 10) and COPD patients (n = 9). METHODS: BEC were treated with OM-85 alone (24 hours) or infected with Rhinovirus. BEC survival was monitored by manual cell counting and Rhinovirus replication by lytic activity. Immuno-blotting and ELISA were used to determine the expression of Rhinovirus interacting proteins: intracellular adhesion molecule (ICAM), major histocompatibility complex class II (MHC-2), complement component C1q receptor (C1q-R), inducible T-Cell co-stimulator (ICOS), its ligand ICOSL, and myeloid differentiation primary response gene 88 (Myd88); as well as for signal transducers Erk1/2, p38, JNK mitogen activated protein kinases MAPK), and cAMP. RESULTS: OM-85 significantly reduced Rhinovirus-induced BEC death and virus replication. OM-85 significantly increased the expression of virus interacting proteins C1q-R and ß-defensin in all 3 probes and groups, which was prevented by either Erk1/2 MAPK or cAMP inhibition. In addition, OM-85 significantly reduced Rhinovirus induced expression of ICAM1 involving p38 MAPK. In BEC OM-85 had no significant effect on the expression of ICOS, ICOSL and MHC-2 membrane proteins nor on the adaptor protein MyD88. CONCLUSION: The OM-85-induced increased of C1q-R and ß-defensin, both important for antigen presentation and phagocytosis, supports its activity in host cell's defence against Rhinovirus infection. [Mh] Termos MeSH primário: Brônquios/metabolismo Extratos Celulares/farmacologia AMP Cíclico/metabolismo Sistema de Sinalização das MAP Quinases Rhinovirus/efeitos dos fármacos Proteínas Virais/metabolismo [Mh] Termos MeSH secundário: Adulto Idoso Idoso de 80 Anos ou mais Brônquios/citologia Estudos de Casos e Controles Células Cultivadas Células Epiteliais/metabolismo Feminino Seres Humanos Masculino Meia-Idade Doença Pulmonar Obstrutiva Crônica/metabolismo Doença Pulmonar Obstrutiva Crônica/patologia Rhinovirus/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Broncho-Vaxom); 0 (Cell Extracts); 0 (Viral Proteins); E0399OZS9N (Cyclic AMP) [Em] Mês de entrada: 1712 [Cu] Atualização por classe: 171226 [Lr] Data última revisão: 171226 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171129 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0188010

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[PMID]: 29049206 [Au] Autor: Saraya T; Kimura H; Kurai D; Ishii H; Takizawa H [Ad] Endereço: aKyorin University School of Medicine, Department of Respiratory Medicine, Mitaka City bInfectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo, Japan. [Ti] Título: The molecular epidemiology of respiratory viruses associated with asthma attacks: A single-center observational study in Japan. [So] Source: Medicine (Baltimore);96(42):e8204, 2017 Oct. [Is] ISSN: 1536-5964 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Few reports have described the significance of viral respiratory infections (VRIs) in exacerbation of asthma in adult patients. The aim of this study was to elucidate the profiles of VRIs in adult patients with asthma along with their molecular epidemiology.A cross-sectional observational study was conducted at Kyorin University Hospital from August 2012 to May 2015. To identify respiratory pathogens in inpatients and outpatients suffering from asthma attacks, RT-PCR/sequencing/phylogenetic analysis methods were applied alongside conventional microbiological methods. Phylogenetic and pairwise distance analyses of 10 viruses were performed.A total of 106 asthma attack patients enrolled in this study in both inpatient (n = 49) and outpatient (n = 57) settings. The total 106 respiratory samples were obtained from nasopharyngeal swab (n = 68) or sputum (n = 38). Among these, patients with virus alone (n = 39), virus and bacterial (n = 5), and bacterial alone (n = 5) were identified. The ratio of virus-positive patients in inpatient or outpatient to the total cases were 31.1% (n = 33) and 10.4% (n = 11), respectively. The frequency of virus-positive patients was significantly higher in inpatients (75.3%, n = 33) than in outpatients (19.3%, n = 11). Major VRIs included human rhinovirus (HRV) (n = 24), human metapneumovirus (hMPV) (n = 9), influenza virus (Inf-V) (n = 8), and respiratory syncytial virus (RSV) (n = 3) infections with seasonal variations. HRV-A and HRV-C were the most commonly detected viruses, with wide genetic divergence on phylogenetic analysis.Asthmatic exacerbations in adults are highly associated with VRIs such as HRV-A or HRV-C, hMPV, RSV, and Inf-V infections with seasonal variations and genetic divergence, but similar frequencies of VRIs occurred in asthma attack patients throughout the seasons. [Mh] Termos MeSH primário: Asma/virologia Vírus de RNA/genética Infecções Respiratórias/epidemiologia Viroses/epidemiologia [Mh] Termos MeSH secundário: Adulto Idoso Infecções Bacterianas/complicações Infecções Bacterianas/epidemiologia Estudos Transversais Feminino Genótipo Seres Humanos Pacientes Internados/estatística & dados numéricos Japão/epidemiologia Masculino Metapneumovirus/genética Metapneumovirus/isolamento & purificação Meia-Idade Nasofaringe/virologia Orthomyxoviridae/genética Orthomyxoviridae/isolamento & purificação Pacientes Ambulatoriais/estatística & dados numéricos Filogenia Vírus de RNA/isolamento & purificação Vírus Sinciciais Respiratórios/genética Vírus Sinciciais Respiratórios/isolamento & purificação Infecções Respiratórias/virologia Rhinovirus/genética Rhinovirus/isolamento & purificação Estações do Ano Escarro/virologia Viroses/complicações [Pt] Tipo de publicação: JOURNAL ARTICLE; OBSERVATIONAL STUDY [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171122 [Lr] Data última revisão: 171122 [Sb] Subgrupo de revista: AIM; IM [Da] Data de entrada para processamento: 171020 [St] Status: MEDLINE [do] DOI: 10.1097/MD.0000000000008204

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[PMID]: 29045416 [Au] Autor: Barlow-Anacker A; Bochkov Y; Gern J; Seroogy CM [Ad] Endereço: Department of Pediatrics, Division of Allergy, Immunology, & Rheumatology, University of Wisconsin School of Medicine and Public Health, Madison, WI, United States of America. [Ti] Título: Neonatal immune response to rhinovirus A16 has diminished dendritic cell function and increased B cell activation. [So] Source: PLoS One;12(10):e0180664, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: BACKGROUND: Rhinovirus infections during infancy account for the majority of respiratory illness health care utilization and are an associated risk factor for subsequent development of allergic asthma. Neonatal type I interferon production is diminished compared to adults after stimulation with TLR agonists. However, broad profiling of immune cell responses to infectious rhinovirus has not been undertaken and we hypothesized that additional immune differences can be identified in neonates. In this study, we undertook a comparative analysis of neonatal and adult blood immune cell responses after in vitro incubation with infectious RV-A16 for 6 and 24 hours. METHODS: Intracellular proinflammatory and type I interferon cytokines along with expression of surface co-stimulatory and maturation markers were measured using multi-parameter flow cytometry. RESULTS: Both circulating myeloid dendritic cell (mDC) and plasmacytoid dendritic cell (pDC) frequency were lower in cord blood. Qualitative and quantitative plasmacytoid dendritic cell IFN-alpha + TNF- alpha responses to rhinovirus were significantly lower in cord pDCs. In cord blood samples, the majority of responsive pDCs were single-positive TNF-alpha producing cells, whereas in adult samples rhinovirus increased double-positive TNF-alpha+IFN-alpha+ pDCs. Rhinovirus upregulated activation and maturation markers on monocytes, mDCs, pDCs, and B cells, but CD40+CD86+ monocytes, mDCs, and pDCs cells were significantly higher in adult samples compared to cord samples. Surprisingly, rhinovirus increased CD40+CD86+ B cells to a significantly greater extent in cord samples compared to adults. CONCLUSIONS: These findings define a number of cell-specific differences in neonatal responses to rhinovirus. This differential age-related immune response to RV may have implications for the immune correlates of protection to viral respiratory illness burden and determination of potential biomarkers for asthma risk. [Mh] Termos MeSH primário: Linfócitos B/imunologia Células Dendríticas/imunologia Imunidade Ativação Linfocitária/imunologia Rhinovirus/imunologia [Mh] Termos MeSH secundário: Adulto Biomarcadores/metabolismo Diferenciação Celular/imunologia Citocinas/metabolismo Antígenos HLA-DR/metabolismo Células HeLa Seres Humanos Recém-Nascido Cordão Umbilical/citologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biomarkers); 0 (Cytokines); 0 (HLA-DR Antigens) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171031 [Lr] Data última revisão: 171031 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171019 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0180664

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