[PMID]: | 29182620 |
[Au] Autor: | Roth M; Pasquali C; Stolz D; Tamm M |
[Ad] Endereço: | Pulmonary Cell Research, DBM University Basel and Pneumology Clinic, University Hospital Basel, Basel, Switzerland. |
[Ti] Título: | Broncho Vaxom (OM-85) modulates rhinovirus docking proteins on human airway epithelial cells via Erk1/2 mitogen activated protein kinase and cAMP. |
[So] Source: | PLoS One;12(11):e0188010, 2017. |
[Is] ISSN: | 1932-6203 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | BACKGROUND: Bronchial epithelial cells (BEC) are primary target for Rhinovirus infection through attaching to cell membrane proteins. OM-85, a bacterial extract, improves recovery of asthma and COPD patients after viral infections, but only part of the mechanism was addressed, by focusing on defined immune cells. OBJECTIVE: We therefore determined the effect of OM-85 on isolated primary human BEC of controls (n = 8), asthma patients (n = 10) and COPD patients (n = 9). METHODS: BEC were treated with OM-85 alone (24 hours) or infected with Rhinovirus. BEC survival was monitored by manual cell counting and Rhinovirus replication by lytic activity. Immuno-blotting and ELISA were used to determine the expression of Rhinovirus interacting proteins: intracellular adhesion molecule (ICAM), major histocompatibility complex class II (MHC-2), complement component C1q receptor (C1q-R), inducible T-Cell co-stimulator (ICOS), its ligand ICOSL, and myeloid differentiation primary response gene 88 (Myd88); as well as for signal transducers Erk1/2, p38, JNK mitogen activated protein kinases MAPK), and cAMP. RESULTS: OM-85 significantly reduced Rhinovirus-induced BEC death and virus replication. OM-85 significantly increased the expression of virus interacting proteins C1q-R and ß-defensin in all 3 probes and groups, which was prevented by either Erk1/2 MAPK or cAMP inhibition. In addition, OM-85 significantly reduced Rhinovirus induced expression of ICAM1 involving p38 MAPK. In BEC OM-85 had no significant effect on the expression of ICOS, ICOSL and MHC-2 membrane proteins nor on the adaptor protein MyD88. CONCLUSION: The OM-85-induced increased of C1q-R and ß-defensin, both important for antigen presentation and phagocytosis, supports its activity in host cell's defence against Rhinovirus infection. |
[Mh] Termos MeSH primário: |
Brônquios/metabolismo Extratos Celulares/farmacologia AMP Cíclico/metabolismo Sistema de Sinalização das MAP Quinases Rhinovirus/efeitos dos fármacos Proteínas Virais/metabolismo
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[Mh] Termos MeSH secundário: |
Adulto Idoso Idoso de 80 Anos ou mais Brônquios/citologia Estudos de Casos e Controles Células Cultivadas Células Epiteliais/metabolismo Feminino Seres Humanos Masculino Meia-Idade Doença Pulmonar Obstrutiva Crônica/metabolismo Doença Pulmonar Obstrutiva Crônica/patologia Rhinovirus/metabolismo
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Broncho-Vaxom); 0 (Cell Extracts); 0 (Viral Proteins); E0399OZS9N (Cyclic AMP) |
[Em] Mês de entrada: | 1712 |
[Cu] Atualização por classe: | 171226 |
[Lr] Data última revisão:
| 171226 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 171129 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1371/journal.pone.0188010 |
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