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[PMID]: 29177545 [Au] Autor: Gu Y; Zhou Y; Shi X; Xin Y; Shan Y; Chen C; Cao T; Fang W; Li X [Ad] Endereço: Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China. [Ti] Título: Porcine teschovirus 2 induces an incomplete autophagic response in PK-15 cells. [So] Source: Arch Virol;163(3):623-632, 2018 Mar. [Is] ISSN: 1432-8798 [Cp] País de publicação: Austria [La] Idioma: eng [Ab] Resumo: Autophagy is a homeostatic process that has been shown to be vital in the innate immune defense against pathogens. However, little is known about the regulatory role of autophagy in porcine teschovirus 2 (PTV-2) replication. In this study, we found that PTV-2 infection induces a strong increase in GFP-LC3 punctae and endogenous LC3 lipidation. However, PTV-2 infection did not enhance autophagic protein degradation. When cellular autophagy was pharmacologically inhibited by wortmannin or 3-methyladenine, PTV-2 replication increased. The increase in virus yield via autophagy inhibition was further confirmed by silencing atg5, which is required for autophagy. Furthermore, PTV-2 replication was suppressed when autophagy was activated by rapamycin. Together, the results suggest that PTV-2 infection activates incomplete autophagy and that autophagy then inhibits further PTV-2 replication. [Mh] Termos MeSH primário: Proteína 5 Relacionada à Autofagia/antagonistas & inibidores Autofagia/efeitos dos fármacos Células Epiteliais/virologia Interações Hospedeiro-Patógeno Teschovirus/efeitos dos fármacos Replicação Viral/efeitos dos fármacos [Mh] Termos MeSH secundário: Adenina/análogos & derivados Adenina/farmacologia Androstadienos/farmacologia Animais Autofagia/genética Proteína 5 Relacionada à Autofagia/genética Proteína 5 Relacionada à Autofagia/metabolismo Linhagem Celular Células Epiteliais/efeitos dos fármacos Células Epiteliais/metabolismo Regulação da Expressão Gênica Genes Reporter Proteínas de Fluorescência Verde/genética Proteínas de Fluorescência Verde/metabolismo Rim Proteínas Associadas aos Microtúbulos/genética Proteínas Associadas aos Microtúbulos/metabolismo RNA Interferente Pequeno/genética RNA Interferente Pequeno/metabolismo Transdução de Sinais Sirolimo/farmacologia Suínos Teschovirus/genética Teschovirus/crescimento & desenvolvimento Teschovirus/metabolismo Replicação Viral/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Androstadienes); 0 (Autophagy-Related Protein 5); 0 (Microtubule-Associated Proteins); 0 (RNA, Small Interfering); 147336-22-9 (Green Fluorescent Proteins); 5142-23-4 (3-methyladenine); JAC85A2161 (Adenine); W36ZG6FT64 (Sirolimus); XVA4O219QW (wortmannin) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180220 [Lr] Data última revisão: 180220 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171128 [St] Status: MEDLINE [do] DOI: 10.1007/s00705-017-3652-2

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[PMID]: 28691894 [Au] Autor: Cano-Gómez C; Fernández-Pinero J; García-Casado MA; Zell R; Jiménez-Clavero MA [Ad] Endereço: 1​Centro de Investigación en Sanidad Animal (CISA)-INIA, Ctra Algete-El Casar s/n, 28130 Valdeolmos, Spain. [Ti] Título: Characterization of PTV-12, a newly described porcine teschovirus serotype: in vivo infection and cross-protection studies. [So] Source: J Gen Virol;98(7):1636-1645, 2017 Jul. [Is] ISSN: 1465-2099 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Porcine teschoviruses (PTVs) constitute 1 of the 31 genera within the Picornaviridae family, comprising at least 13 genetic types (PTV-1 to PTV-13), of which only 11 (PTV-1 to PTV-11) have been recognized as serotypes to date. Specific for swine and wild boars, most PTVs are usually non-pathogenic, but some viral variants cause severe disorders in the central nervous system (Teschen disease) or milder signs (Talfan disease), as well as reproductive, digestive and respiratory disorders and skin lesions. Previous studies revealed a high diversity of teschoviruses circulating in Spanish pig populations. Phylogenetic analysis performed with these sequences and others available in GenBank disclosed 13 clusters, 11 of which corresponded to the known PTV serotypes, and 1 of 2 additional groups is represented by isolate CC25, whose full-length genomic sequence has been obtained. This group is new to science, and was putatively named PTV-12. Here, a complete characterization of this isolate is presented, including the experimental infection of minipigs to assess tissue tropism and possible pathogenicity in vivo in the host species. In addition, using this experimental animal model, we investigated whether a pre-existing infection with this PTV-12 isolate could confer cross-protection against infection with a heterotypic PTV-1 virulent strain. Based on phylogenetic analysis and serological data, we propose CC25 as the prototype strain of a new teschovirus serotype, PTV-12. [Mh] Termos MeSH primário: Proteção Cruzada/imunologia Infecções por Picornaviridae/imunologia Doenças dos Suínos/imunologia Porco Miniatura/virologia Teschovirus/classificação Teschovirus/imunologia Tropismo Viral/fisiologia [Mh] Termos MeSH secundário: Animais Anticorpos Neutralizantes/sangue Anticorpos Antivirais/sangue Infecções por Picornaviridae/virologia Sorogrupo Sorotipagem Espanha Suínos Doenças dos Suínos/virologia Teschovirus/genética Teschovirus/isolamento & purificação Viremia/virologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antibodies, Neutralizing); 0 (Antibodies, Viral) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170804 [Lr] Data última revisão: 170804 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170711 [St] Status: MEDLINE [do] DOI: 10.1099/jgv.0.000822

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[PMID]: 27810445 [Au] Autor: Jones TH; Muehlhauser V [Ad] Endereço: Agriculture and Agri-Food Canada, Lacombe Research Centre, 6000 C & E Trail, Lacombe, Alberta T4L 1W1, Canada. Electronic address: Tineke.Jones@agr.gc.ca. [Ti] Título: F-coliphages, porcine adenovirus and porcine teschovirus as potential indicator viruses of fecal contamination for pork carcass processing. [So] Source: Int J Food Microbiol;241:237-243, 2017 Jan 16. [Is] ISSN: 1879-3460 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: There are concerns about the zoonotic transmission of viruses through undercooked pork products. There is a lack of information on suitable indicator viruses for fecal contamination with pathogenic enteric viruses in the meat processing chain. The study compared the incidence and levels of contamination of hog carcasses with F-coliphages, porcine teschovirus (PTV), and porcine adenovirus (PAdV) at different stages of the dressing process to assess their potential as indicator viruses of fecal contamination. One hundred swab samples (200cm ) were collected from random sites on hog carcasses at 4 different stages of the dressing process and from retail pork over the span of a year from 2 pork processing plants (500/plant). Viable F-coliphages, PAdV DNA and PTV RNA were each detected on ≥99% of the incoming carcasses at both plants and were traceable through the pork processing chain. Significant correlations were observed between viable F-coliphages and PAdV DNA and between F-coliphages and PTV RNA but not between PAdV DNA and PTV RNA at the various stages of pork processing. Detection of viable F-coliphages was more sensitive than genomic copies of PAdV and PTV at low levels of contamination, making F-coliphages a preferred indicator in the pork slaughter process as it also provides an indication of infectivity. For plant A, F-RNA coliphages were detected in 25%, 63%, and 21% of carcass swabs after pasteurization, evisceration, and retail pork products, respectively. For plant B, F-coliphages were detected in 33%, 25%, and 13% of carcass swabs after skinning, evisceration, and retail pork samples, respectively. Viable F-RNA coliphages were genotyped. Viable F-RNA GII and GIII were generally not detected at the earlier stages of the slaughter process but they were detected on 13% of carcasses after evisceration and 2% of retail pork samples at plant A, which raises concerns of potential food handler contamination during pork processing. Consumers could be at risk when consuming undercooked meat contaminated with pathogenic enteric viruses. [Mh] Termos MeSH primário: Adenovirus Suínos/isolamento & purificação Colífagos/isolamento & purificação Fezes/virologia Contaminação de Alimentos/análise Carne/virologia Teschovirus/isolamento & purificação [Mh] Termos MeSH secundário: Adenovirus Suínos/genética Animais Colífagos/genética Manipulação de Alimentos Suínos Teschovirus/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170917 [Lr] Data última revisão: 170917 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161105 [St] Status: MEDLINE

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[PMID]: 27352927 [Au] Autor: Tang X; Liu X; Tao G; Qin M; Yin G; Suo J; Suo X [Ad] Endereço: State Key Laboratory of Agrobiotechnology and College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China. [Ti] Título: "Self-cleaving" 2A peptide from porcine teschovirus-1 mediates cleavage of dual fluorescent proteins in transgenic Eimeria tenella. [So] Source: Vet Res;47(1):68, 2016 Jun 28. [Is] ISSN: 1297-9716 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The "self-cleaving" 2A sequence of picornavirus, which mediates ribosome-skipping events, enables the generation of two or more separate peptide products from one mRNA containing one or more "self-cleaving" 2A sequences. In this study, we introduced a single 2A sequence of porcine teschovirus-1 (P2A) linked to two fluorescent protein genes, the enhanced yellow fluorescent protein (EYFP) gene and the red fluorescent protein (RFP) gene, in a single cassette into transgenic Eimeria tenella (EtER). As expected, we obtained two separated protein molecules rather than a fused protein, although the two molecules were translated from the same mRNA carrying a single "self-cleaving" 2A sequence. Importantly, RFP led by a secretion signal was secreted into parasitophorous vacuoles, while EYFP localized mainly to the nucleus of EtER. Our results demonstrate that the "self-cleaving" 2A sequence actively mediated cleavage of polyproteins in the apicomplexan parasite E. tenella. [Mh] Termos MeSH primário: Proteínas de Bactérias/genética Eimeria tenella/genética Proteínas Luminescentes/genética Teschovirus/genética [Mh] Termos MeSH secundário: Animais Proteínas de Bactérias/metabolismo Galinhas/parasitologia Eimeria tenella/metabolismo Proteínas Luminescentes/metabolismo Organismos Geneticamente Modificados/genética Organismos Geneticamente Modificados/metabolismo Teschovirus/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Bacterial Proteins); 0 (Luminescent Proteins); 0 (red fluorescent protein); 0 (yellow fluorescent protein, Bacteria) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170926 [Lr] Data última revisão: 170926 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160630 [St] Status: MEDLINE [do] DOI: 10.1186/s13567-016-0351-z

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[PMID]: 26860913 [Au] Autor: Villanova F; Cui S; Ai X; Leal É [Ad] Endereço: Instituto de Ciências Biológicas, Federal University of Pará, Avenida Augusto Corrêa, 1, CEP 66075-000, Belém, Pará, Brazil. [Ti] Título: Analysis of full-length genomes of porcine teschovirus (PTV) and the effect of purifying selection on phylogenetic trees. [So] Source: Arch Virol;161(5):1199-208, 2016 May. [Is] ISSN: 1432-8798 [Cp] País de publicação: Austria [La] Idioma: eng [Ab] Resumo: To study the outcome of natural selection using phylogenetic trees, we analyzed full-length genome sequences of porcine teschovirus (PTV). PTV belongs to the family Picornaviridae and has a positive-stranded RNA genome, the replication of which is carried out by the error-prone viral RNA-dependent RNA polymerase. The viral RNA encodes a single polyprotein that is cleaved into structural (i.e., L, VP4, VP2, VP3 and VP1) and nonstructural proteins (i.e., 2A, 2B, 2C, 3A, 3B, and 3C). A high degree of genetic diversity was found based on the pairwise nucleotide distances and on the mean ratio of the number of nonsynonymous (dN) and synonymous (dS) substitutions (dN/dS) in the structural genes. Conversely, the diversity of the nonstructural genes was lower. The differences in genetic diversity between the structural and nonstructural genomic regions were likely due to strong purifying selection; consequently, the estimates of phylogenies were also discordant among these genes. In particular, maximum-likelihood and Bayesian methods generated short-branched trees when loci that are under strong purifying selection were used. These findings indicate that even in an RNA virus with an intrinsically high mutation rate, a strong purifying selection will curb genetic diversity and should be considered an important source of bias in future studies based on phylogenetic methods. [Mh] Termos MeSH primário: Genoma Viral/genética Teschovirus/genética [Mh] Termos MeSH secundário: Evolução Molecular Genes Virais/genética Variação Genética/genética Filogenia Recombinação Genética/genética Alinhamento de Sequência Teschovirus/isolamento & purificação [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Em] Mês de entrada: 1608 [Cu] Atualização por classe: 160421 [Lr] Data última revisão: 160421 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160211 [St] Status: MEDLINE [do] DOI: 10.1007/s00705-015-2744-0

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[PMID]: 26711042 [Au] Autor: Tsai AT; Kuo CC; Kuo YC; Yang JL; Chang CY; Wang FI [Ad] Endereço: School of Veterinary Medicine, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan; Bestar Laboratories (S) Pte. Ltd. (Vaccine Research Center), Singapore. [Ti] Título: The urinary shedding of porcine teschovirus in endemic field situations. [So] Source: Vet Microbiol;182:150-5, 2016 Jan 15. [Is] ISSN: 1873-2542 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Porcine teschoviruses (PTVs) belong to the genus Teschovirus within the family Picornaviridae. PTVs are universal contaminants in pig herds in endemic and multi-infection statuses. Previous research has demonstrated PTV antigens and nucleic acid in renal glomeruli and tubular epithelia, suggesting the possibility that PTVs might be shed and transmitted via urine. The study aimed to demonstrate, in the context of pathogenesis, the presence of PTVs in the urine of naturally infected pigs. Viral loads of fluid and tissue samples quantified by an established qRT-PCR showed detection rates of 100% by head and in urine, feces, plasma and nasal swabs, and 38% in kidney. As predicted, PTVs were present in urine at 10(4.02 ± 1.45) copies/100 µl volume, equivalent to 17% of that in plasma. No significant differences were observed between healthy and culled pigs or among the 7 sampled herds. The presence of PTVs in urine was further substantiated by molecular serotyping. In particular, PTV-10 was identified in the urine of 3 piglets from 3 separate herds, consistent with the most prevalent serotype found in this study, and in plasma. The urine mixes with feces to form slurry making it easier for PTV to spread and contaminate the environment. [Mh] Termos MeSH primário: Doenças Endêmicas/veterinária Infecções por Picornaviridae/veterinária Doenças dos Suínos/virologia Teschovirus/fisiologia Urina/virologia [Mh] Termos MeSH secundário: Animais Infecções por Picornaviridae/genética Infecções por Picornaviridae/transmissão Infecções por Picornaviridae/virologia Sorogrupo Sus scrofa/virologia Suínos Doenças dos Suínos/genética Doenças dos Suínos/transmissão Teschovirus/genética Teschovirus/isolamento & purificação Carga Viral Eliminação de Partículas Virais [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Em] Mês de entrada: 1606 [Cu] Atualização por classe: 151229 [Lr] Data última revisão: 151229 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 151230 [St] Status: MEDLINE

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[PMID]: 25957149 [Au] Autor: Lin YJ; Liu WT; Stark H; Huang CT [Ad] Endereço: Department of Biochemical Science and Technology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taipei, Taiwan. [Ti] Título: Expression of enterovirus 71 virus-like particles in transgenic enoki (Flammulina velutipes). [So] Source: Appl Microbiol Biotechnol;99(16):6765-74, 2015 Aug. [Is] ISSN: 1432-0614 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: No commercial vaccines are currently available for enterovirus 71 (EV71) infection. Oral virus-like particle (VLP) vaccines are regarded as a better choice for prevention from food-borne diseases compared with injected whole virus vaccines. Unfortunately, the application of oral VLP vaccines produced from transgenic plants was limited due to the concerns of gene contamination. Alternatively, using transgenic mushrooms retains the advantages of transgenic plants and tremendously reduce risks of gene contamination. Polycistronic expression vectors harboring the glyceraldehyde-3-phospho-dehydrogenase promoter to codrive EV71 structural protein P1 and protease 3C using the 2A peptide of porcine teschovirus-1 were constructed and introduced into Flammulina velutipes via Agrobacterium tumefaciens-mediated transformation. The analyses of the genomic PCR, Southern blotting, and RT-PCR showed that the genes of P1 and 3C were integrated into the chromosomal DNA through a single insertion, and their resulting mRNAs were transcribed. The Western blotting analysis combined with LC-MS/MS demonstrated that EV71 VLPs were composed of the four subunit proteins digested from P1 polyprotein by 3C protease. Through the use of a single particle electron microscope, images of 1705 particles with diameter similar to the EV71 viron were used for 3D reconstruction. Protrusions were observed on the surface in the 2D class averages, and a 3D reconstruction of the VLPs was obtained. In conclusion, EV71 VLPs were successfully produced in transgenic F. velutipes using a polycistronic expression strategy, which indicates that this approach is promising for the development of oral vaccines produced in mushrooms. [Mh] Termos MeSH primário: Enterovirus Humano A/genética Flammulina/metabolismo Proteínas Virais/metabolismo Virossomos/metabolismo [Mh] Termos MeSH secundário: Agrobacterium tumefaciens Southern Blotting Western Blotting Cromatografia Líquida Flammulina/genética Perfilação da Expressão Gênica Imagem Tridimensional Microscopia Eletrônica Reação em Cadeia da Polimerase Reação em Cadeia da Polimerase em Tempo Real Espectrometria de Massas em Tandem Teschovirus/genética Transformação Genética Proteínas Virais/genética Virossomos/genética [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Viral Proteins); 0 (Virosomes) [Em] Mês de entrada: 1604 [Cu] Atualização por classe: 150724 [Lr] Data última revisão: 150724 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150510 [St] Status: MEDLINE [do] DOI: 10.1007/s00253-015-6588-z

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[PMID]: 25686406 [Au] Autor: Olschewski K; Kämmer E; Stöckel S; Bocklitz T; Deckert-Gaudig T; Zell R; Cialla-May D; Weber K; Deckert V; Popp J [Ad] Endereço: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University, Helmholtzweg 4, 07743 Jena, Germany. juergen.popp@ipht-jena.de. [Ti] Título: A manual and an automatic TERS based virus discrimination. [So] Source: Nanoscale;7(10):4545-52, 2015 Mar 14. [Is] ISSN: 2040-3372 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Rapid techniques for virus identification are more relevant today than ever. Conventional virus detection and identification strategies generally rest upon various microbiological methods and genomic approaches, which are not suited for the analysis of single virus particles. In contrast, the highly sensitive spectroscopic technique tip-enhanced Raman spectroscopy (TERS) allows the characterisation of biological nano-structures like virions on a single-particle level. In this study, the feasibility of TERS in combination with chemometrics to discriminate two pathogenic viruses, Varicella-zoster virus (VZV) and Porcine teschovirus (PTV), was investigated. In a first step, chemometric methods transformed the spectral data in such a way that a rapid visual discrimination of the two examined viruses was enabled. In a further step, these methods were utilised to perform an automatic quality rating of the measured spectra. Spectra that passed this test were eventually used to calculate a classification model, through which a successful discrimination of the two viral species based on TERS spectra of single virus particles was also realised with a classification accuracy of 91%. [Mh] Termos MeSH primário: Herpesvirus Humano 3/química Análise Espectral Raman/métodos Teschovirus/química [Mh] Termos MeSH secundário: Animais Seres Humanos Suínos [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Em] Mês de entrada: 1511 [Cu] Atualização por classe: 150227 [Lr] Data última revisão: 150227 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150217 [St] Status: MEDLINE [do] DOI: 10.1039/c4nr07033j

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[PMID]: 25552321 [Au] Autor: Sun H; Gao H; Chen M; Lan D; Hua X; Wang C; Yuan C; Yang Z; Cui L [Ad] Endereço: Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Minhang District, Shanghai, 200240, China. [Ti] Título: New serotypes of porcine teschovirus identified in Shanghai, China. [So] Source: Arch Virol;160(3):831-5, 2015 Mar. [Is] ISSN: 1432-8798 [Cp] País de publicação: Austria [La] Idioma: eng [Ab] Resumo: Teschoviruses are widely endemic and commonly found in pig fecal samples. In this study, we collected fecal specimens from various pig herds and genotyped them based on the VP1 gene. Of 322 samples, 276 were positive, giving a PTV infectivity rate of 85.7 %. PTV4 was the most common serotype found in Shanghai, followed by PTV8 and PTV10. Interestingly, Some Shanghai strains belonging to a new PTV serotype were also isolated. In phylogenetic analysis, PTV SH8 did not correspond to any known serotype. PTV4 and PTV6 showed similar levels of sequence identity to PTV SH8. These data suggest that PTV SH8 is a new serotype, distinct from the new serotype PTV wild boar/WB2C-TV/2011/HUN, which clusters with PTV SH2, SH10, and SH25. [Mh] Termos MeSH primário: Fezes/virologia Sorogrupo Suínos/virologia Teschovirus/classificação Teschovirus/isolamento & purificação [Mh] Termos MeSH secundário: Animais China Análise por Conglomerados Genótipo Dados de Sequência Molecular Filogenia RNA Viral/genética Análise de Sequência de DNA Homologia de Sequência Teschovirus/genética Proteínas Estruturais Virais/genética [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (RNA, Viral); 0 (Viral Structural Proteins) [Em] Mês de entrada: 1504 [Cu] Atualização por classe: 150223 [Lr] Data última revisão: 150223 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150102 [St] Status: MEDLINE [do] DOI: 10.1007/s00705-014-2326-6

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[PMID]: 25461604 [Au] Autor: Jones TH; Muehlhauser V [Ad] Endereço: Agriculture and Agri-Food Canada, Lacombe Research Centre, 6000 C & E Trail, Lacombe, Alberta T4L 1W1, Canada. Electronic address: Tineke.Jones@agr.gc.ca. [Ti] Título: Survival of Porcine teschovirus as a surrogate virus on pork chops during storage at 2°C. [So] Source: Int J Food Microbiol;194:21-4, 2015 Feb 02. [Is] ISSN: 1879-3460 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Due to the lack of an efficient cultivation system, little is known about the stability and inactivation of hepatitis E virus (HEV). In addition, there is a lack of information on which cultivable virus(es) are suitable as model or surrogate viruses for HEV. Murine norovirus (MNV) and F-RNA coliphage MS2 are potential surrogates and F-RNA coliphages are a potential indicator for enteric viruses. However, the numbers of F-RNA coliphages excreted by swine are relatively low. In contrast, Porcine teschovirus (PTV) is cultivable and is excreted abundantly. PTV is readily detected on swine carcasses and the potential of PTV as a viral indicator of fecal contamination on hog carcasses is currently being explored, however, there is no information on the environmental stability of PTV. The survival of PTV was determined on vacuum packaged pork chops during storage at 2°C using cultivation and molecular techniques and compared to published data on the survival of MNV and MS2 under similar conditions. Viable PTV was reduced by ≥1.8log units compared to a reduction of 0.6 log genomic copies after 7weeks. The viability data indicates that PTV is less stable than MS2 and MNV during storage at 2°C whereas similar reductions in genomic copies were observed for all 3 viruses. This study provides data on the survival of PTV on pork and insight on the potential of PTV as a surrogate for HEV in the pork processing chain. [Mh] Termos MeSH primário: Temperatura Baixa Microbiologia de Alimentos Carne/virologia Teschovirus/fisiologia [Mh] Termos MeSH secundário: Animais Manipulação de Alimentos Viabilidade Microbiana Suínos Fatores de Tempo [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Em] Mês de entrada: 1503 [Cu] Atualização por classe: 141219 [Lr] Data última revisão: 141219 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 141203 [St] Status: MEDLINE

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1			PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

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