Base de dados : MEDLINE
Pesquisa : B04.820.630 [Categoria DeCS]
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[PMID]:29339070
[Au] Autor:Wang R; Li Y; Zhou Z; Liu Q; Zeng L; Xiao T
[Ad] Endereço:Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha 410128, China; College of Life and Environment Sciences, Hunan University of Arts and Science, Changde, Hunan 415000, China.
[Ti] Título:Involvement of interferon regulatory factor 3 from the barbel chub Squaliobarbus curriculus in the immune response against grass carp reovirus.
[So] Source:Gene;648:5-11, 2018 Mar 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The barbel chub Squaliobarbus curriculus is an important commercial fish species in China, and has shown significant resistance to grass carp reovirus (GCRV). In this study, the cDNA sequence of interferon regulatory factors 3 (IRF3) from Squaliobarbus curriculus, designated as ScIRF3, was cloned, and its effect against GCRV was investigated. The full-length 1837 base pair (bp) cDNA of ScIRF3 contained a complete open reading frame of 1374 bp and encoded a putative polypeptide of 457 amino acid residues. The ScIRF3 protein contained conserved domains, including an N-terminal DNA-binding domain, a C-terminal IRF association domain, and a serine-rich domain. Phylogenetic analysis showed that ScIRF3 was closely clustered with IRF3s from Carassius auratus and Ctenopharyngodon idellus. Quantitative real-time polymerase chain reaction analysis showed that the expression levels of ScIRF3 in Squaliobarbus curriculus were the highest in the spleen and lowest in the muscle. After GCRV infection, expression levels of both ScIRF3 and type I interferon (IFN) were initially up-regulated and subsequently down-regulated in the spleen and intestine. Correlation analysis showed that the expression level of type I IFN is significantly positively correlated with that of ScIRF3 (Pearson correlation coefficient: 0.883, P: 0.004) in the intestine. The expression level of type I IFN was also significantly up-regulated and the GCRV titer was significantly decreased (P < .05) in GCRV-infected ScIRF3-overexpressing Ctenopharyngodon idellus kidney cells. These results indicate that ScIRF3 may play a role in the type I IFN immune response against GCRV in Squaliobarbus curriculus and can also inhibit GCRV replication in Ctenopharyngodon idellus kidney cells.
[Mh] Termos MeSH primário: Cyprinidae/imunologia
Doenças dos Peixes/imunologia
Proteínas de Peixes/imunologia
Fator Regulador 3 de Interferon/imunologia
Reoviridae/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Cyprinidae/metabolismo
Cyprinidae/virologia
Doenças dos Peixes/metabolismo
Doenças dos Peixes/virologia
Proteínas de Peixes/classificação
Proteínas de Peixes/metabolismo
Perfilação da Expressão Gênica/métodos
Interações Hospedeiro-Patógeno/imunologia
Fator Regulador 3 de Interferon/genética
Fator Regulador 3 de Interferon/metabolismo
Interferon Tipo I/genética
Interferon Tipo I/imunologia
Interferon Tipo I/metabolismo
Filogenia
Reoviridae/fisiologia
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 0 (Interferon Regulatory Factor-3); 0 (Interferon Type I)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE


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[PMID]:29193300
[Au] Autor:Lan H; Hong X; Huang R; Lin X; Li Q; Li K; Zhou T
[Ad] Endereço:School of Biological Sciences and Biotechnology, Minnan Normal University, Zhangzhou, PR China.
[Ti] Título:RNA interference-mediated knockdown and virus-induced suppression of Troponin C gene adversely affect the behavior or fitness of the green rice leafhopper, Nephotettix cincticeps.
[So] Source:Arch Insect Biochem Physiol;97(2), 2018 Feb.
[Is] ISSN:1520-6327
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The green rice leafhopper, Nephotettix cincticeps, is a major rice pest in Southeast Asia and Southern China. Novel control strategies must be explored to control the rice pest. Behavior or fitness regulation of insect by modulating the Troponin C (TnC) may be a novel strategy in the comprehensive management of the insect pest. However, characterizations and functions of TnC, especially regarding effect of its RNA interference-mediated gene knockdown on the behavior or fitness of N. cincticeps remain unknown. Here, we successfully cloned and characterized TnC gene from N. cincticeps (Nc-TnC). We demonstrated that Nc-TnC ubiquitously transcribed at all development stages and special tissues in adult insects, with relative higher levels at the adult stage and in the intestinal canal. Microinjection- or oral membrane feeding-based transient knockdown of Nc-TnC adversely affected the performance or fitness, such as the decreased survival, feeding capacity, weight, and fecundity of N. cincticeps. Furthermore, we revealed that the expression of Nc-TnC was suppressed by its interaction with rice dwarf virus-encoded nonstructural protein 10, which ultimately affected detrimentally the corresponding parameters of the performance or fitness of N. cincticeps. In conclusion, our data deepen understanding of Nc-TnC functions during the development of and viral infection in N. cincticeps. It imply Nc-TnC may serve as a potential target for N. cincticeps control in future.
[Mh] Termos MeSH primário: Hemípteros/fisiologia
Reoviridae/fisiologia
Troponina C/fisiologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Feminino
Aptidão Genética
Hemípteros/virologia
Controle de Insetos
Larva/metabolismo
Estágios do Ciclo de Vida
Interferência de RNA
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Troponin C)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1002/arch.21438


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[PMID]:28468626
[Au] Autor:Xu Q; Liu H; Yuan P; Zhang X; Chen Q; Jiang X; Zhou Y
[Ad] Endereço:Institute of Plant Protection; Jiangsu Academy of Agricultural Sciences; Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing, Jiangsu, People's Republic of China.
[Ti] Título:Development of a simplified RT-PCR without RNA isolation for rapid detection of RNA viruses in a single small brown planthopper (Laodelphax striatellus Fallén).
[So] Source:Virol J;14(1):90, 2017 05 03.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The small brown planthopper (SBPH) is an important pest of cereal crops and acts as a transmission vector for multiple RNA viruses. Rapid diagnosis of virus in the vector is crucial for efficient forecast and control of viral disease. Reverse transcription polymerase chain reaction (RT-PCR) is a rapid, sensitive and reliable method for virus detection. The traditional RT-PCR contains a RNA isolation step and is widely used for virus detection in insect. However, using the traditional RT-PCR for detecting RNA virus in individual SBPHs becomes challenging because of the expensive reagents and laborious procedure associated with RNA isolation when processing a large number of samples. RESULTS: We established a simplified RT-PCR method without RNA isolation for RNA virus detection in a single SBPH. This method is achieved by grinding a single SBPH in sterile water and using the crude extract directly as the template for RT-PCR. The crude extract containing the virus RNA can be prepared in approximately two minutes. Rice stripe virus (RSV), rice black streaked dwarf virus (RBSDV) and Himetobi P virus (HiPV) were successfully detected using this simplified method. The detection results were validated by sequencing and dot immunobinding assay, indicating that this simplified method is reliable for detecting different viruses in insects. The evaluation of the sensitivity of this method showed that both RSV and HiPV can be detected when the cDNA from the crude extract was diluted up to 10 fold. Compared to the traditional RT-PCR with RNA isolation, the simplified RT-PCR method greatly reduces the sample processing time, decreases the detection cost, and improves the efficiency by avoiding RNA isolation. CONCLUSIONS: A simplified RT-PCR method is developed for rapid detection of RNA virus in a single SBPH without the laborious RNA isolation step. It offers a convenient alternative to the traditional RT-PCR method.
[Mh] Termos MeSH primário: Hemípteros/virologia
Insetos Vetores/virologia
Vírus de RNA/isolamento & purificação
RNA Viral/isolamento & purificação
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
[Mh] Termos MeSH secundário: Animais
DNA Complementar/isolamento & purificação
Dicistroviridae/genética
Dicistroviridae/isolamento & purificação
Immunoblotting/métodos
Insetos/virologia
Vírus de Plantas/genética
Vírus de Plantas/isolamento & purificação
RNA Viral/análise
Reoviridae/genética
Reoviridae/isolamento & purificação
Sensibilidade e Especificidade
Tenuivirus/genética
Tenuivirus/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (RNA, Viral)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1186/s12985-017-0732-6


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[PMID]:29211815
[Au] Autor:Berger AK; Yi H; Kearns DB; Mainou BA
[Ad] Endereço:Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia, United States of America.
[Ti] Título:Bacteria and bacterial envelope components enhance mammalian reovirus thermostability.
[So] Source:PLoS Pathog;13(12):e1006768, 2017 Dec.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Enteric viruses encounter diverse environments as they migrate through the gastrointestinal tract to infect their hosts. The interaction of eukaryotic viruses with members of the host microbiota can greatly impact various aspects of virus biology, including the efficiency with which viruses can infect their hosts. Mammalian orthoreovirus, a human enteric virus that infects most humans during childhood, is negatively affected by antibiotic treatment prior to infection. However, it is not known how components of the host microbiota affect reovirus infectivity. In this study, we show that reovirus virions directly interact with Gram positive and Gram negative bacteria. Reovirus interaction with bacterial cells conveys enhanced virion thermostability that translates into enhanced attachment and infection of cells following an environmental insult. Enhanced virion thermostability was also conveyed by bacterial envelope components lipopolysaccharide (LPS) and peptidoglycan (PG). Lipoteichoic acid and N-acetylglucosamine-containing polysaccharides enhanced virion stability in a serotype-dependent manner. LPS and PG also enhanced the thermostability of an intermediate reovirus particle (ISVP) that is associated with primary infection in the gut. Although LPS and PG alter reovirus thermostability, these bacterial envelope components did not affect reovirus utilization of its proteinaceous cellular receptor junctional adhesion molecule-A or cell entry kinetics. LPS and PG also did not affect the overall number of reovirus capsid proteins σ1 and σ3, suggesting their effect on virion thermostability is not mediated through altering the overall number of major capsid proteins on the virus. Incubation of reovirus with LPS and PG did not significantly affect the neutralizing efficiency of reovirus-specific antibodies. These data suggest that bacteria enhance reovirus infection of the intestinal tract by enhancing the thermal stability of the reovirus particle at a variety of temperatures through interactions between the viral particle and bacterial envelope components.
[Mh] Termos MeSH primário: Bacillus subtilis/fisiologia
Enterócitos/virologia
Escherichia coli K12/fisiologia
Infecções por Reoviridae/virologia
Reoviridae/fisiologia
[Mh] Termos MeSH secundário: Acetilglucosamina/análogos & derivados
Acetilglucosamina/metabolismo
Acetilglucosamina/toxicidade
Bacillus subtilis/metabolismo
Bacillus subtilis/ultraestrutura
Bacillus subtilis/virologia
Células CACO-2
Endotoxinas/metabolismo
Endotoxinas/toxicidade
Enterócitos/efeitos dos fármacos
Enterócitos/microbiologia
Enterócitos/patologia
Escherichia coli K12/metabolismo
Escherichia coli K12/ultraestrutura
Escherichia coli K12/virologia
Microbioma Gastrointestinal
Células HeLa
Temperatura Alta
Seres Humanos
Lipopolissacarídeos/metabolismo
Lipopolissacarídeos/toxicidade
Proteínas Luminescentes/genética
Proteínas Luminescentes/metabolismo
Microscopia Eletrônica de Transmissão
Peptidoglicano/metabolismo
Peptidoglicano/toxicidade
RNA/metabolismo
Estabilidade de RNA/efeitos dos fármacos
Proteínas Recombinantes/metabolismo
Reoviridae/química
Reoviridae/efeitos dos fármacos
Reoviridae/patogenicidade
Infecções por Reoviridae/metabolismo
Infecções por Reoviridae/microbiologia
Infecções por Reoviridae/patologia
Ácidos Teicoicos/metabolismo
Ácidos Teicoicos/toxicidade
Vírion/química
Vírion/patogenicidade
Vírion/fisiologia
Ligação Viral/efeitos dos fármacos
Internalização do Vírus/efeitos dos fármacos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Endotoxins); 0 (Lipopolysaccharides); 0 (Luminescent Proteins); 0 (Peptidoglycan); 0 (RNA, recombinant); 0 (Recombinant Proteins); 0 (Teichoic Acids); 0 (red fluorescent protein); 56411-57-5 (lipoteichoic acid); 63231-63-0 (RNA); 67924-63-4 (endotoxin, Escherichia coli); V956696549 (Acetylglucosamine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180109
[Lr] Data última revisão:
180109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006768


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[PMID]:28461211
[Au] Autor:Luo K; Li Y; Xia L; Hu W; Gao W; Guo L; Tian G; Qi Z; Yuan H; Xu Q
[Ad] Endereço:Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Jingzhou 434020, China.
[Ti] Título:Analysis of the expression patterns of the novel large multigene TRIM gene family (finTRIM) in zebrafish.
[So] Source:Fish Shellfish Immunol;66:224-230, 2017 Jul.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tripartite motif (TRIM) proteins are receiving increased research interest because of their roles in a wide range of cellular biological processes in innate immunity. In zebrafish (Danio rerio), the functions of the finTRIM (ftr) family are unclear. In the present study, we investigated the expression pattern of ftr12, ftr51, ftr67, ftr82, ftr83, and ftr84 in zebrafish for the first time. The results showed that ftr12, ftr67, and ftr84 are maternally expressed in the oocyte and highly expressed at the early stage (0-4 hpf) of embryo (P < 0.05), suggesting their involvement in the embryonic innate defense system. The ftr82 gene was highly expressed at 8 hpf (P < 0.05), which implied that the embryos could synthesize their own immunity-related mRNAs. However, ftr51 and ftr83 were highest at 8 hpf (2.33 and 51.53 relative to ß-actin respectively) and might mediate embryonic development. The expression levels of ftr12, ftr51, and ftr67 were highest in the gill, intestines, and liver, respectively. Ftr82, ftr83, and ftr84 were predominantly expressed in the kidney, suggesting that these finTRIMs might play roles in both immunity and non-immunity-related tissue compartments. Zebrafish embryonic fibroblast (ZF4) cells were infected with Grass carp reovirus (GCRV) and Spring viremia of carp virus (SVCV). During GCRV infection, the expression of ftr12 was significantly upregulated from 12 h to 24 h; and ftr51 and ftr67 increased from 3 h to 12 h. The expressions of ftr82, ftr83, and ftr84 were only upregulated at 12 h, 12 h, and 24 h, respectively. All of these genes were significantly downregulated at 48 h (P < 0.05). Challenge with SVCV upregulated the expressions of ftr12 and ftr51 at 12 h and 48 h (P < 0.05), respectively, and ftr67 reached its highest expression level at 3 h. ftr82 showed only a slight upregulation at 6 h and 48 h, and ftr83 and ftr84 were consecutively increased, reaching their highest levels at 12 h (P < 0.05). Meanwhile, ftr67 and ftr83 were significantly downregulated at 48 h (P < 0.05). Our research demonstrated that ftr12, ftr51, ftr67, ftr82, ftr83, and ftr84 probably have important roles in innate immune responses and in non-immunity-related tissues.
[Mh] Termos MeSH primário: Doenças dos Peixes/genética
Expressão Gênica
Imunidade Inata/genética
Família Multigênica
Proteínas com Motivo Tripartido/genética
Proteínas de Peixe-Zebra/genética
Peixe-Zebra
[Mh] Termos MeSH secundário: Animais
Doenças dos Peixes/imunologia
Doenças dos Peixes/virologia
Expressão Gênica/imunologia
Perfilação da Expressão Gênica/veterinária
Reoviridae/fisiologia
Infecções por Reoviridae/genética
Infecções por Reoviridae/imunologia
Infecções por Reoviridae/veterinária
Rhabdoviridae/fisiologia
Infecções por Rhabdoviridae/genética
Infecções por Rhabdoviridae/imunologia
Infecções por Rhabdoviridae/veterinária
Análise de Sequência de DNA/veterinária
Proteínas com Motivo Tripartido/metabolismo
Proteínas de Peixe-Zebra/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Tripartite Motif Proteins); 0 (Zebrafish Proteins)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171215
[Lr] Data última revisão:
171215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


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[PMID]:28756871
[Au] Autor:Cohn DE; Sill MW; Walker JL; O'Malley D; Nagel CI; Rutledge TL; Bradley W; Richardson DL; Moxley KM; Aghajanian C
[Ad] Endereço:Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Ohio State University College of Medicine, Columbus, OH, United States. Electronic address: David.Cohn@osumc.edu.
[Ti] Título:Randomized phase IIB evaluation of weekly paclitaxel versus weekly paclitaxel with oncolytic reovirus (Reolysin®) in recurrent ovarian, tubal, or peritoneal cancer: An NRG Oncology/Gynecologic Oncology Group study.
[So] Source:Gynecol Oncol;146(3):477-483, 2017 Sep.
[Is] ISSN:1095-6859
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To assess whether the addition of oncolytic reovirus (Reolysin®) to weekly paclitaxel prolonged progression-free survival (PFS) in the treatment of women with recurrent or persistent ovarian, tubal or primary peritoneal cancer. PATIENTS AND METHODS: Patients with recurrent or persistent epithelial ovarian, tubal, or peritoneal carcinoma, measurable or detectable disease, and three or fewer prior regimens were randomly assigned to paclitaxel (80mg/m intravenously days 1, 8, and 15 every 4weeks) or the combination of paclitaxel (80mg/m intravenously days 1, 8, and 15) plus reovirus 3×10 TCID /day intravenously on days 1-5, both every 4weeks until disease progression or toxicity. The primary end point was PFS. The study was designed with 80% power for a one-sided alternative at a 10% level of significance to detect a reduction in the hazard by 37.5%. RESULTS: The study accrued 108 patients, 100 of whom were evaluable for toxicity. Median PFS was 4.3months for paclitaxel and 4.4months for paclitaxel plus reovirus (hazard ratio, 1.11; 90% two-sided CI, 0.78 to 1.59; one-sided P=0.687). The proportion responding (overall response rate) to paclitaxel was 20% among 45 patients with measurable disease receiving paclitaxel alone, and 17.4% among the 46 patients treated with the combination. The asymptotic relative probability of responding was 0.87 (90% CI, 0.42 to 1.79). Severe adverse events were more common in the combination regimen than in paclitaxel arm for severe neutropenia (grade≥4, 12% versus 0%), and severe respiratory adverse events (grade≥3, 25% versus 2%). No deaths were considered treatment related. CONCLUSION: The addition of reovirus to weekly paclitaxel in the treatment of women with recurrent or persistent ovarian, tubal or peritoneal cancer did not sufficiently reduce the hazard of progression or death to warrant further investigation.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/uso terapêutico
Carcinoma/terapia
Neoplasias das Tubas Uterinas/terapia
Recidiva Local de Neoplasia/terapia
Neoplasias Epiteliais e Glandulares/terapia
Terapia Viral Oncolítica
Neoplasias Ovarianas/terapia
Paclitaxel/uso terapêutico
Neoplasias Peritoneais/terapia
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Terapia Combinada/efeitos adversos
Intervalo Livre de Doença
Feminino
Seres Humanos
Meia-Idade
Neutropenia/etiologia
Terapia Viral Oncolítica/efeitos adversos
Vírus Oncolíticos
Paclitaxel/efeitos adversos
Estudos Prospectivos
Reoviridae
Doenças Respiratórias/etiologia
[Pt] Tipo de publicação:CLINICAL TRIAL, PHASE II; COMPARATIVE STUDY; JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); P88XT4IS4D (Paclitaxel)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE


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[PMID]:28700950
[Au] Autor:Ahmed MMS; Ji W; Wang M; Bian S; Xu M; Wang W; Zhang J; Xu Z; Yu M; Liu Q; Zhang C; Zhang H; Tang S; Gu M; Yu H
[Ad] Endereço:Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China; Department of Crop Protection, Faculty of Agriculture, U
[Ti] Título:Transcriptional changes of rice in response to rice black-streaked dwarf virus.
[So] Source:Gene;628:38-47, 2017 Sep 10.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Rice black-streaked dwarf virus (RBSDV), a member of the genus Fijivirus in the family Reoviridae, causes significant economic losses in rice production in China and many other Asian countries. Although a great deal of effort has been made to elucidate the interactions among the virus, insect vectors, host and environmental conditions, few RBSDV proteins involved in pathogenesis have been identified, and the biological basis of disease development in rice remains largely unknown. Transcriptomic information associated with the disease development in rice would be helpful to unravel the biological mechanism. To determine how the rice transcriptome changes in response to RBSDV infection, we carried out RNA-Seq to perform a genome-wide gene expression analysis of a susceptible rice cultivar KTWYJ3. The transcriptomes of RBSDV-infected samples were compared to those of RBSDV-free (healthy) at two time points (time points are represented by group I and II). The results derived from the differential expression analysis in RBSDV-infected libraries vs. healthy ones in group I revealed that 102 out of a total of 281 significant differentially expressed genes (DEGs) were up-regulated and 179 DEGs were down-regulated. Of the 2592 identified DEGs in group II, 1588 DEGs were up-regulated and 1004 DEGs were down-regulated. A total of 66 DEGs were commonly identified in both groups. Of these 66 DEGs, expression patterns for 36 DEGs were similar in both groups. Our analysis demonstrated that some genes related to disease defense and stress resistance were up-regulated while genes associated with chloroplast were down-regulated in response to RBSDV infection. In addition, some genes associated with plant-height were differentially expressed. This result indicates those genes might be involved in dwarf symptoms caused by RBSDV. Taken together, our results provide a genome-wide transcriptome analysis for rice plants in response to RBSDV infection which may contribute to the understanding of the regulatory mechanisms involved in rice-RBSDV interaction and the biological basis of rice black-streaked dwarf disease development in rice.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica de Plantas
Oryza/genética
Doenças das Plantas/genética
Vírus de Plantas/fisiologia
Reoviridae/fisiologia
Transcrição Genética
[Mh] Termos MeSH secundário: DNA de Plantas
Perfilação da Expressão Gênica
Oryza/virologia
Doenças das Plantas/virologia
RNA de Plantas
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant); 0 (RNA, Plant)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE


  8 / 4008 MEDLINE  
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[PMID]:28668346
[Au] Autor:He L; Hu X; Zhu M; Liang Z; Chen F; Zhu L; Kuang S; Cao G; Xue R; Gong C
[Ad] Endereço:School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
[Ti] Título:Identification and characterization of vp7 gene in Bombyx mori cytoplasmic polyhedrosis virus.
[So] Source:Gene;627:343-350, 2017 Sep 05.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The genome of Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) contains 10 double stranded RNA segments (S1-S10). The segment 7 (S7) encodes 50kDa protein which is considered as a structural protein. The expression pattern and function of p50 in the virus life cycle are still unclear. In this study, the viral structural protein 7 (VP7) polyclonal antibody was prepared with immunized mouse to explore the presence of small VP7 gene-encoded proteins in Bombyx mori cytoplasmic polyhedrosis virus. The expression pattern of vp7 gene was investigated by its overexpression in BmN cells. In addition to VP7, supplementary band was identified with western blotting technique. The virion, BmCPV infected cells and midguts were also examined using western blotting technique. 4, 2 and 5 bands were detected in the corresponding samples, respectively. The replication of BmCPV genome in the cultured cells and midgut of silkworm was decreased by reducing the expression level of vp7 gene using RNA interference. In immunoprecipitation experiments, using a polyclonal antiserum directed against the VP7, one additional shorter band in BmCPV infected midguts was detected, and then the band was analyzed with mass spectrum (MS), the MS results showed thatone candidate interacted protein (VP7 voltage-dependent anion-selective channel-like isoform, VDAC) was identified from silkworm. We concluded that the novel viral product was generated with a leaky scanning mechanism and the VDAC may be an interacted protein with VP7.
[Mh] Termos MeSH primário: Proteínas do Nucleocapsídeo/genética
Reoviridae/genética
[Mh] Termos MeSH secundário: Animais
Bombyx/virologia
Clonagem Molecular
Intestinos/virologia
Proteínas do Nucleocapsídeo/química
Proteínas do Nucleocapsídeo/metabolismo
Reoviridae/metabolismo
Reoviridae/fisiologia
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nucleocapsid Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170703
[St] Status:MEDLINE


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[PMID]:28494021
[Au] Autor:Tao T; Zhou CJ; Wang Q; Chen XR; Sun Q; Zhao TY; Ye JC; Wang Y; Zhang ZY; Zhang YL; Guo ZJ; Wang XB; Li DW; Yu JL; Han CG
[Ad] Endereço:State Key Laboratory for Agro-biotechnology and Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing, People's Republic of China.
[Ti] Título:Rice black streaked dwarf virus P7-2 forms a SCF complex through binding to Oryza sativa SKP1-like proteins, and interacts with GID2 involved in the gibberellin pathway.
[So] Source:PLoS One;12(5):e0177518, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:As a core subunit of the SCF complex that promotes protein degradation through the 26S proteasome, S-phase kinase-associated protein 1 (SKP1) plays important roles in multiple cellular processes in eukaryotes, including gibberellin (GA), jasmonate, ethylene, auxin and light responses. P7-2 encoded by Rice black streaked dwarf virus (RBSDV), a devastating viral pathogen that causes severe symptoms in infected plants, interacts with SKP1 from different plants. However, whether RBSDV P7-2 forms a SCF complex and targets host proteins is poorly understood. In this study, we conducted yeast two-hybrid assays to further explore the interactions between P7-2 and 25 type I Oryza sativa SKP1-like (OSK) proteins, and found that P7-2 interacted with eight OSK members with different binding affinity. Co-immunoprecipitation assay further confirmed the interaction of P7-2 with OSK1, OSK5 and OSK20. It was also shown that P7-2, together with OSK1 and O. sativa Cullin-1, was able to form the SCF complex. Moreover, yeast two-hybrid assays revealed that P7-2 interacted with gibberellin insensitive dwarf2 (GID2) from rice and maize plants, which is essential for regulating the GA signaling pathway. It was further demonstrated that the N-terminal region of P7-2 was necessary for the interaction with GID2. Overall, these results indicated that P7-2 functioned as a component of the SCF complex in rice, and interaction of P7-2 with GID2 implied possible roles of the GA signaling pathway during RBSDV infection.
[Mh] Termos MeSH primário: Giberelinas/metabolismo
Complexos Multiproteicos/metabolismo
Oryza/metabolismo
Oryza/virologia
Proteínas de Plantas/metabolismo
Reoviridae/metabolismo
Proteínas Quinases Associadas a Fase S/metabolismo
Proteínas Virais/metabolismo
[Mh] Termos MeSH secundário: Imunoprecipitação
Folhas de Planta/metabolismo
Ligação Proteica
Reprodutibilidade dos Testes
Tabaco/metabolismo
Técnicas do Sistema de Duplo-Híbrido
Zea mays
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gibberellins); 0 (Multiprotein Complexes); 0 (Plant Proteins); 0 (S-Phase Kinase-Associated Proteins); 0 (Viral Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170512
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177518


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[PMID]:28446676
[Au] Autor:Lu LF; Li S; Wang ZX; Du SQ; Chen DD; Nie P; Zhang YA
[Ad] Endereço:State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
[Ti] Título:Grass Carp Reovirus VP41 Targets Fish MITA To Abrogate the Interferon Response.
[So] Source:J Virol;91(14), 2017 Jul 15.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Although fish possess an efficient interferon (IFN) system to defend against aquatic virus infection, grass carp reovirus (GCRV) still causes hemorrhagic disease in grass carp. To date, GCRV's strategy for evading the fish IFN response is still unknown. Here, we report that GCRV VP41 inhibits fish IFN production by suppressing the phosphorylation of mediator of IFN regulatory factor 3 (IRF3) activation (MITA). First, the activation of the IFN promoter (IFNpro) stimulated by mitochondrial antiviral signaling protein (MAVS) and MITA was decreased by the overexpression of VP41, whereas such activation induced by TANK-binding kinase 1 (TBK1) was not affected. Second, VP41 was colocalized in the cellular endoplasmic reticulum (ER) and associated with MITA. Furthermore, as a phosphorylation substrate of TBK1, VP41 significantly decreased the phosphorylation of MITA. Truncation assays indicated that the transmembrane (TM) region of VP41 was indispensable for the suppression of IFNpro activity. Finally, after infection with GCRV, VP41 blunted the transcription of host IFN and facilitated viral RNA synthesis. Taken together, our findings suggest that GCRV VP41 prevents the fish IFN response by attenuating the phosphorylation of MITA for viral evasion. MITA is thought to act as an adaptor protein to facilitate the phosphorylation of IRF3 by TBK1 upon viral infection, and it plays a critical role in innate antiviral responses. Here, we report that GCRV VP41 colocalizes with MITA at the ER and reduces MITA phosphorylation by acting as a decoy substrate of TBK1, thus inhibiting IFN production. These findings reveal GCRV's strategy for evading the host IFN response for the first time.
[Mh] Termos MeSH primário: Interações Hospedeiro-Patógeno
Evasão da Resposta Imune
Fatores Imunológicos/antagonistas & inibidores
Interferons/antagonistas & inibidores
Reoviridae/patogenicidade
Proteínas Virais/metabolismo
[Mh] Termos MeSH secundário: Animais
Carpas/virologia
Linhagem Celular
Análise Mutacional de DNA
Seres Humanos
Deleção de Sequência
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunologic Factors); 0 (Viral Proteins); 9008-11-1 (Interferons)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE



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