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  1 / 221 MEDLINE  
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[PMID]:28288679
[Au] Autor:Wu Y; Cui L; Zhu E; Zhou W; Wang Q; Wu X; Wu B; Huang Y; Liu HJ
[Ad] Endereço:College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
[Ti] Título:Muscovy duck reovirus σNS protein triggers autophagy enhancing virus replication.
[So] Source:Virol J;14(1):53, 2017 Mar 14.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Muscovy duck reovirus (MDRV) causes high morbidity and mortality in Muscovy ducklings at 10 days old and can persist in an infected flock until the ducklings of 6 weeks old. It shares common physicochemical properties with avian reovirus (ARV) and differs in coding assignment and pathogenicity. The ARV p17 protein has been shown to trigger autophagy via activation multiple signaling pathways, which benefits virus replication. Since MDRV lacks the p17 protein, whether and how MDRV induces autophagy remains unknown. The aim of this study was to explore whether MDRV induces autophagy and which viral proteins are involved in MDRV-induced autophagy. METHODS: The autophagosome-like structures in MDRV-infected cells was observed under transmission electron microscopy. MDRV-induced autophagy was examined by analyzing the LC3-II level and phosphorylated form of mammalian target of rapamycin (mTOR) by Western blot assays. The effects of 3-methyladenine, rapamycin, chloroquine on viral yields were measured with quantitative(q) real-time reverse transcription (RT)-polymerase chain reaction (PCR) and 50% tissue culture infective dose (TCID ) assays, respectively. Additionally, to determine which viral protein is responsible for MDRV-induced autophagy, both p10.8- and σNS-encoding genes of MDRV were cloned into the pCI-neo-flag vector and transfected into DF-1 cells for detection of LC3-II. RESULTS: The typical double-membrane vesicles containing cytoplasmic inclusions were visible in MDRV-infected immortalized chicken embryo fibroblast (DF-1) cells under transmission electron microscopy. Both primary Muscovy duck embryo fibroblasts (MDEF) and DF-1 cells infected with MDRV exhibited a significant increased levels of LC3-II accompanied with downregulation of phosphorylated form of mTOR, further confirming that MDRV is capable of inducing autophagy. Autophagy could be suppressed by 3-methylademine and induced by rapamycin and chloroquine. Furthermore, we found that σNS induces an increased levels of LC3-II, suggesting that the MDRV σNS protein is one of viral proteins involved in induction of autophagy. Both qRT-PCR and TCID assays showed that virus yield was increased in rapamycin treated DF-1 cells following MDRV infection. Conversely, when infected cells were pretreated with chloroquine, virus yield was decreased. CONCLUSIONS: The MDRV σNS nonstructural protein is responsible for MDRV-induced autophagy and benefits virus replication.
[Mh] Termos MeSH primário: Autofagia
Orthoreovirus Aviário/fisiologia
Proteínas não Estruturais Virais/metabolismo
Replicação Viral
[Mh] Termos MeSH secundário: Animais
Autofagossomos/ultraestrutura
Western Blotting
Linhagem Celular
Galinhas
Microscopia Eletrônica de Transmissão
Proteínas Associadas aos Microtúbulos/análise
Reação em Cadeia da Polimerase em Tempo Real
Serina-Treonina Quinases TOR/análise
Carga Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Microtubule-Associated Proteins); 0 (Viral Nonstructural Proteins); EC 2.7.1.1 (TOR Serine-Threonine Kinases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170823
[Lr] Data última revisão:
170823
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170315
[St] Status:MEDLINE
[do] DOI:10.1186/s12985-017-0722-8


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[PMID]:28013385
[Au] Autor:Kumar D; Chauhan TK; Agarwal RK; Dhama K; Goswami PP; Mariappan AK; Tiwari AK; Mishra BP
[Ad] Endereço:Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, 243122, UP, India. deep_biotek@yahoo.com.
[Ti] Título:A double-stranded probe coupled with isothermal amplification for qualitative and quantitative detection of avian reovirus.
[So] Source:Arch Virol;162(4):979-985, 2017 Apr.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:We applied a probe-based real-time loop-mediated isothermal amplification (Cy5-RTqLAMP) technique targeting the avian reovirus (ARV) S3 gene to develop a rapid, sensitive, and specific method for virus detection and quantification. This test specifically detected the presence of ARV, but not other viruses or bacteria present in clinical or artificially spiked samples, including Newcastle disease virus, infectious bursal disease virus, fowl adenovirus, Marek's disease virus, Escherichia coli, and Salmonella spp. This test can detect ARV in less than one hour with an analytical sensitivity of 10 viral gene copies and 1 fg of total cDNA. The Cy5-RTqLAMP does not yield false positive results and is 100 times more sensitive than conventional PCR. This test was shown to be able to detect the presence of ARV in clinical samples. A similar strategy may be used for detection of other important human and animal viral pathogens.
[Mh] Termos MeSH primário: DNA Viral/genética
Técnicas de Amplificação de Ácido Nucleico/métodos
Orthoreovirus Aviário/isolamento & purificação
Doenças das Aves Domésticas/virologia
Infecções por Reoviridae/veterinária
[Mh] Termos MeSH secundário: Animais
Galinhas
Primers do DNA/genética
Seres Humanos
Técnicas de Amplificação de Ácido Nucleico/instrumentação
Orthoreovirus Aviário/classificação
Orthoreovirus Aviário/genética
Reação em Cadeia da Polimerase/instrumentação
Reação em Cadeia da Polimerase/métodos
Doenças das Aves Domésticas/diagnóstico
Infecções por Reoviridae/diagnóstico
Infecções por Reoviridae/virologia
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Viral)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170315
[Lr] Data última revisão:
170315
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161226
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-016-3184-1


  3 / 221 MEDLINE  
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[PMID]:27928915
[Au] Autor:Kaithal B; Jindal N; Kumar P; Mor SK
[Ti] Título:Detection and molecular characterization of enteric viruses in enteritis-affected commercial broiler chickens in India.
[So] Source:Acta Virol;60(4):361-371, 2016.
[Is] ISSN:0001-723X
[Cp] País de publicação:Slovakia
[La] Idioma:eng
[Ab] Resumo:A study was conducted to detect and characterize the enteric viruses (chicken astrovirus, avian nephritis virus and avian orthoreovirus) present in flocks of commercial broiler chickens suffering from enteritis in Haryana, India. The intestinal contents were collected from 65 enteritis-affected flocks (cases) and tested by reverse transcription PCR (RT-PCR). Of these 65 cases, 35 (53.80%) were positive for a single virus and 26 (40.00%) for two viruses. The remaining four samples were negative for all three viruses tested. Of the 65 cases, 57 were positive for chicken astrovirus (CAstV) while 30 cases had avian nephritis virus (ANV). None of the cases were positive for orthoreovirus. Comparison of 12 CAstVs of this study with previously published CAstV sequences revealed nucleotide identities ranging from 73.20 to 98.00%. The nucleotide identities ranged between 83.10-95.50% when nine ANVs of this study were compared with previously reported ANV sequences. The amino acid sequences of CAstVs in comparison to previously published sequences revealed certain unique changes. Phylogeny based on polymerase gene revealed that CAstVs and ANVs of this study were under the same monophyletic clade. In conclusion, a large number of broiler chicken flocks experiencing enteritis were positive for CAstV and ANV by RT-PCR. The presence of more than one enteric virus in enteritis-affected flocks and changes at the genetic level in these viruses may affect the severity of disease.
[Mh] Termos MeSH primário: Infecções por Astroviridae/veterinária
Avastrovirus/isolamento & purificação
Enterite/veterinária
Orthoreovirus Aviário/isolamento & purificação
Doenças das Aves Domésticas/virologia
Infecções por Reoviridae/veterinária
[Mh] Termos MeSH secundário: Animais
Infecções por Astroviridae/epidemiologia
Infecções por Astroviridae/virologia
Avastrovirus/classificação
Avastrovirus/genética
Galinhas/virologia
Enterite/virologia
Índia/epidemiologia
Orthoreovirus Aviário/classificação
Orthoreovirus Aviário/genética
Filogenia
Doenças das Aves Domésticas/epidemiologia
Infecções por Reoviridae/epidemiologia
Infecções por Reoviridae/virologia
Perus/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170627
[Lr] Data última revisão:
170627
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161209
[St] Status:MEDLINE


  4 / 221 MEDLINE  
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[PMID]:27796116
[Au] Autor:Parsons NJ; Gous TA; Schaefer AM; Vanstreels RE
[Ad] Endereço:Southern African Foundation for the Conservation of Coastal Birds, Bloubergrant; Bayworld Centre for Research and Education, Port Elizabeth. nolaparsons@yahoo.co.uk.
[Ti] Título:Health evaluation of African penguins (Spheniscus demersus) in southern Africa.
[So] Source:Onderstepoort J Vet Res;83(1):e1-e13, 2016 Sep 20.
[Is] ISSN:2219-0635
[Cp] País de publicação:South Africa
[La] Idioma:eng
[Ab] Resumo:The African penguin (Spheniscus demersus) is an endangered seabird that breeds along the coast of Namibia and South Africa, and disease surveillance was identified as a priority for its conservation. Aiming for the establishment of baseline data on the presence of potential pathogens in this species, a comprehensive health assessment (blood smear examination, haematology, biochemistry and serology) was conducted on samples obtained from 578 African penguins at 11 breeding colonies and a rehabilitation centre. There were 68 penguins that were seropositive for at least one of seven pathogens tested: avian encephalomyelitis virus, avian infectious bronchitis virus, avian reovirus, infectious bursal disease virus, Newcastle disease virus, Mycoplasma gallisepticum and Mycoplasma synoviae. All samples were seronegative for avian influenza virus subtypes H5 and H7 and infectious laryngotracheitis virus. The apparent prevalence of Babesia sp. and Borrelia sp. in blood smears was consistent with previous studies. Babesia-infected individuals had a regenerative response of the erythrocytic lineage, an active inflammatory response and hepatic function impairment. These findings indicate that African penguins may be exposed to conservation-significant pathogens in the wild and encourage further studies aiming for the direct detection and/or isolation of these microorganisms.
[Mh] Termos MeSH primário: Doenças das Aves/epidemiologia
Spheniscidae
[Mh] Termos MeSH secundário: Animais
Babesia/isolamento & purificação
Doenças das Aves/sangue
Doenças das Aves/microbiologia
Vírus da Encefalomielite Aviária/isolamento & purificação
Vírus da Bronquite Infecciosa/isolamento & purificação
Mycoplasma/isolamento & purificação
Vírus da Doença de Newcastle/isolamento & purificação
Orthoreovirus Aviário/isolamento & purificação
África do Sul/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170206
[Lr] Data última revisão:
170206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161101
[St] Status:MEDLINE
[do] DOI:10.4102/ojvr.v83i1.1147


  5 / 221 MEDLINE  
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[PMID]:27603133
[Au] Autor:Chiu HC; Huang WR; Liao TL; Wu HY; Munir M; Shih WL; Liu HJ
[Ad] Endereço:Institute of Molecular Biology, National Chung Hsing University, Taichung 402, Taiwan.
[Ti] Título:Suppression of Vimentin Phosphorylation by the Avian Reovirus p17 through Inhibition of CDK1 and Plk1 Impacting the G2/M Phase of the Cell Cycle.
[So] Source:PLoS One;11(9):e0162356, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The p17 protein of avian reovirus (ARV) causes cell cycle retardation in a variety of cell lines; however, the underlying mechanism(s) by which p17 regulates the cell cycle remains largely unknown. We demonstrate for the first time that p17 interacts with CDK1 and vimentin as revealed by reciprocal co-immunoprecipitation and GST pull-down assays. Both in vitro and in vivo studies indicated that direct interaction of p17 and CDK1/vimentin was mapped within the amino terminus (aa 1-60) of p17 and central region (aa 27-118) of CDK1/vimentin. Furthermore, p17 was found to occupy the Plk1-binding site within the vimentin, thereby blocking Plk1 recruitment to CDK1-induced vimentin phosphorylation at Ser 56. Interaction of p17 to CDK1 or vimentin interferes with CDK1-catalyzed phosphorylation of vimentin at Ser 56 and subsequently vimentin phosphorylation at Ser 82 by Plk1. Furthermore, we have identified upstream signaling pathways and cellular factor(s) targeted by p17 and found that p17 regulates inhibitory phosphorylation of CDK1 and blocks vimentin phosphorylation at Ser 56 and Ser 82. The p17-mediated inactivation of CDK1 is dependent on several mechanisms, which include direct interaction with CDK1, p17-mediated suppression of Plk1 by activating the Tpr/p53 and ATM/Chk1/PP2A pathways, and p17-mediated cdc25C degradation via an ubiquitin- proteasome pathway. Additionally, depletion of p53 with a shRNA as well as inhibition of ATM and vimentin by inhibitors diminished virus yield while Tpr and CDK1 knockdown increased virus yield. Taken together, results demonstrate that p17 suppresses both CDK1 and Plk1functions, disrupts vimentin phosphorylation, causes G2/M cell cycle arrest and thus benefits virus replication.
[Mh] Termos MeSH primário: Proteína Quinase CDC2/metabolismo
Proteínas de Ciclo Celular/metabolismo
Divisão Celular
Fase G2
Orthoreovirus Aviário/metabolismo
Proteínas Serina-Treonina Quinases/metabolismo
Proteínas Proto-Oncogênicas/metabolismo
Vimentina/metabolismo
Proteínas Virais/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas Mutadas de Ataxia Telangiectasia/metabolismo
Proliferação Celular
Cercopithecus aethiops
Quinase do Ponto de Checagem 1/metabolismo
Embrião de Galinha
Inibidor de Quinase Dependente de Ciclina p21/metabolismo
Regulação para Baixo
Imunoprecipitação
Modelos Biológicos
Complexo de Proteínas Formadoras de Poros Nucleares/metabolismo
Fosforilação
Fosfosserina/metabolismo
Complexo de Endopeptidases do Proteassoma/metabolismo
Ligação Proteica
Domínios e Motivos de Interação entre Proteínas
Proteólise
Transdução de Sinais
Transfecção
Proteína Supressora de Tumor p53/metabolismo
Ubiquitina/metabolismo
Regulação para Cima
Células Vero
Proteínas Virais/química
Replicação Viral
Fosfatases cdc25/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cell Cycle Proteins); 0 (Cyclin-Dependent Kinase Inhibitor p21); 0 (Nuclear Pore Complex Proteins); 0 (Proto-Oncogene Proteins); 0 (Tumor Suppressor Protein p53); 0 (Ubiquitin); 0 (Vimentin); 0 (Viral Proteins); 17885-08-4 (Phosphoserine); EC 2.7.11.1 (Ataxia Telangiectasia Mutated Proteins); EC 2.7.11.1 (Checkpoint Kinase 1); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 2.7.11.1 (polo-like kinase 1); EC 2.7.11.22 (CDC2 Protein Kinase); EC 3.1.3.48 (cdc25 Phosphatases); EC 3.4.25.1 (Proteasome Endopeptidase Complex)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170804
[Lr] Data última revisão:
170804
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160908
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0162356


  6 / 221 MEDLINE  
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[PMID]:27527781
[Au] Autor:Li N; Hong T; Wang Y; Wang Y; Yu K; Cai Y; Liu S; Wei L; Chai T
[Ad] Endereço:College of Veterinary Medicine, Shandong Agricultural University, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, 61 Daizong Road, Tai'an 271000, Shandong Province, China; Collaborative Innovation Centre for the Origin and Control of Emerging Infectious Disea
[Ti] Título:The pathogenicity of novel duck reovirus in Cherry Valley ducks.
[So] Source:Vet Microbiol;192:181-185, 2016 Aug 30.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The novel duck reovirus (NDRV) is an emerging, contagious infection. To better realize the pathogenic mechanism of NDRV in ducks, an infection experiment was conducted. The resulting data demonstrated that typical gross lesions were observed in the infected ducks. NDRV was able to replicate in various tissues, leading to these pathological lesions, especially on the liver and spleen. Real-time quantitative PCR showed that the expression of most innate immune-related genes was up-regulated and the antiviral innate immune response could be established in both the liver and spleen. This study indicates that NDRV is a pantropic virus. To resist viral infection, several pathogen recognition receptors can cooperatively recognize NDRV and initiate innate immunity, but the responses are different between different tissues. As far as we know, this is the first systematic investigation of the pathogenicity of NDRV in Cherry Valley ducks based on the host's innate immunity, and these data will provide new insights into the further study of the disease.
[Mh] Termos MeSH primário: Patos
Orthoreovirus Aviário/patogenicidade
Doenças das Aves Domésticas/virologia
[Mh] Termos MeSH secundário: Animais
Regulação da Expressão Gênica/imunologia
Imunidade Inata
Fígado/imunologia
Fígado/patologia
Doenças das Aves Domésticas/patologia
RNA Viral/isolamento & purificação
Baço/imunologia
Baço/patologia
Carga Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171008
[Lr] Data última revisão:
171008
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160817
[St] Status:MEDLINE


  7 / 221 MEDLINE  
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[PMID]:27523295
[Au] Autor:Niu X; Chen H; Yang J; Yu X; Ti J; Wang A; Diao Y
[Ad] Endereço:Research Institute of Poultry Disease, Agricultural University of Shan Dong province, Tai'an, Shandong, China.
[Ti] Título:Development of a TaqMan-based real-time PCR assay for the detection of Novel GPV.
[So] Source:J Virol Methods;237:32-37, 2016 Nov.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The newly emerged disease, duck beak atrophy and dwarfism syndrome (BADS), is caused by novel goose parovirus (N-GPV). Although N-GPV infection has severe consequences, few methods for detecting this virus have been developed. Therefore, the availability of rapid and reliable molecular diagnostic methods would aid future studies of this novel virus. Clinical specimens from 138 suspected cases of N-GPV infection and 120 cloacal swabs from breeding ducks were used in this study. The targeted sequence of N-GPV cloned into the pMD18-T vector was used to generate the N-GPV DNA standard curve. The specificity of the assay was validated using duck plague virus, GPV, duck hepatitis virus, avian influenza virus, duck reovirus, tembusu virus, and fowl adenovirus. The lowest limit of detection was 8.8×10 copies/µL with a good linear standard curve (Y=-3.3682X+37.220, R =0.9953) over a wide range of input DNA, of which the concentration was between 8.8×10 to 8.8×10 copies/µL. The results show that the real-time PCR assay is a highly sensitive, specific, reproducible, and versatile method for quantitatively detecting N-GPV DNA, and thus can be used to detect this virus, thereby facilitating epidemiological investigations of animals with BADS.
[Mh] Termos MeSH primário: Gansos/virologia
Infecções por Parvoviridae/veterinária
Parvovirus/isolamento & purificação
Doenças das Aves Domésticas/diagnóstico
Reação em Cadeia da Polimerase em Tempo Real/métodos
[Mh] Termos MeSH secundário: Animais
Vírus da Leucose Aviária/genética
Patos/virologia
Adenovirus A das Aves/genética
Vírus da Hepatite do Pato/genética
Limite de Detecção
Orthoreovirus Aviário/genética
Infecções por Parvoviridae/virologia
Parvovirus/genética
Doenças das Aves Domésticas/virologia
Sensibilidade e Especificidade
Proteínas Virais/genética
Proteínas Virais/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160816
[St] Status:MEDLINE


  8 / 221 MEDLINE  
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[PMID]:27440902
[Au] Autor:Lostalé-Seijo I; Martínez-Costas J; Benavente J
[Ad] Endereço:Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares and Departamento de Bioquímica e Bioloxía Molecular, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
[Ti] Título:Response of Three Different Viruses to Interferon Priming and Dithiothreitol Treatment of Avian Cells.
[So] Source:J Virol;90(18):8328-40, 2016 Sep 15.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: We have previously shown that the replication of avian reovirus (ARV) in chicken cells is much more resistant to interferon (IFN) than the replication of vesicular stomatitis virus (VSV) or vaccinia virus (VV). In this study, we have investigated the role that the double-stranded RNA (dsRNA)-activated protein kinase (PKR) plays in the sensitivity of these three viruses toward the antiviral action of chicken interferon. Our data suggest that while interferon priming of avian cells blocks vaccinia virus replication by promoting PKR activation, the replication of vesicular stomatitis virus appears to be blocked at a pretranslational step. Our data further suggest that the replication of avian reovirus in chicken cells is quite resistant to interferon priming because this virus uses strategies to downregulate PKR activation and also because translation of avian reovirus mRNAs is more resistant to phosphorylation of the alpha subunit of initiation factor eIF2 than translation of their cellular counterparts. Our results further reveal that the avian reovirus protein sigmaA is able to prevent PKR activation and that this function is dependent on its double-stranded RNA-binding activity. Finally, this study demonstrates that vaccinia virus and avian reovirus, but not vesicular stomatitis virus, express/induce factors that counteract the ability of dithiothreitol to promote eIF2 phosphorylation. Our data demonstrate that each of the three different viruses used in this study elicits distinct responses to interferon and to dithiothreitol-induced eIF2 phosphorylation when infecting avian cells. IMPORTANCE: Type I interferons constitute the first barrier of defense against viral infections, and one of the best characterized antiviral strategies is mediated by the double-stranded RNA-activated protein kinase R (PKR). The results of this study revealed that IFN priming of avian cells has little effect on avian reovirus (ARV) replication but drastically diminishes the replication of vaccinia virus (VV) and vesicular stomatitis virus (VSV) by PKR-dependent and -independent mechanisms, respectively. Our data also demonstrate that the dsRNA-binding ability of ARV protein sigmaA plays a key role in the resistance of ARV toward IFN by preventing PKR activation. Our findings will contribute to improve the current understanding of the interaction of viruses with the host's innate immune system. Finally, it would be of interest to uncover the mechanisms that allow avian reovirus transcripts to be efficiently translated under conditions (moderate eIF2 phosphorylation) that block the synthesis of cellular proteins.
[Mh] Termos MeSH primário: Antivirais/metabolismo
Interferons/metabolismo
Orthoreovirus Aviário/fisiologia
Vírus Vaccinia/fisiologia
Vesiculovirus/fisiologia
Replicação Viral/efeitos dos fármacos
eIF-2 Quinase/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Galinhas
Ditiotreitol/metabolismo
Fator de Iniciação 2 em Eucariotos/metabolismo
Interações Hospedeiro-Patógeno
Fosforilação
Processamento de Proteína Pós-Traducional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Eukaryotic Initiation Factor-2); 9008-11-1 (Interferons); EC 2.7.11.1 (eIF-2 Kinase); T8ID5YZU6Y (Dithiothreitol)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170508
[Lr] Data última revisão:
170508
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160722
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.01175-16


  9 / 221 MEDLINE  
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[PMID]:27233800
[Au] Autor:Xie L; Xie Z; Huang L; Fan Q; Luo S; Huang J; Deng X; Xie Z; Zeng T; Zhang Y; Wang S
[Ad] Endereço:Department of Biotechnology, Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostics, Guangxi Veterinary Research Institute, 51 Youai North Road, Nanning, 530001, China.
[Ti] Título:Avian reovirus σA and σNS proteins activate the phosphatidylinositol 3-kinase-dependent Akt signalling pathway.
[So] Source:Arch Virol;161(8):2243-8, 2016 Aug.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:The present study was conducted to identify avian reovirus (ARV) proteins that can activate the phosphatidylinositol 3-kinase (PI3K)-dependent Akt pathway. Based on ARV protein amino acid sequence analysis, σA, σNS, µA, µB and µNS were identified as putative proteins capable of mediating PI3K/Akt pathway activation. The recombinant plasmids σA-pcAGEN, σNS-pcAGEN, µA-pcAGEN, µB-pcAGEN and µNS-pcAGEN were constructed and used to transfect Vero cells, and the expression levels of the corresponding genes were quantified by immunofluorescence and Western blot analysis. Phosphorylated Akt (P-Akt) levels in the transfected cells were measured by flow cytometry and Western blot analysis. The results showed that the σA, σNS, µA, µB and µNS genes were expressed in Vero cells. σA-expressing and σNS-expressing cells had higher P-Akt levels than negative control cells, pcAGEN-expressing cells and cells designed to express other proteins (i.e., µA, µB and µNS). Pre-treatment with the PI3K inhibitor LY294002 inhibited Akt phosphorylation in σA- and σNS-expressing cells. These results indicate that the σA and σNS proteins can activate the PI3K/Akt pathway.
[Mh] Termos MeSH primário: Doenças das Aves/enzimologia
Doenças das Aves/virologia
Orthoreovirus Aviário/metabolismo
Fosfatidilinositol 3-Quinase/metabolismo
Proteínas Proto-Oncogênicas c-akt/metabolismo
Proteínas de Ligação a RNA/metabolismo
Infecções por Reoviridae/enzimologia
Infecções por Reoviridae/veterinária
Proteínas do Core Viral/metabolismo
Proteínas Virais Reguladoras e Acessórias/metabolismo
[Mh] Termos MeSH secundário: Animais
Doenças das Aves/genética
Cercopithecus aethiops
Regulação Viral da Expressão Gênica
Interações Hospedeiro-Patógeno
Orthoreovirus Aviário/genética
Fosfatidilinositol 3-Quinase/genética
Proteínas Proto-Oncogênicas c-akt/genética
Proteínas de Ligação a RNA/genética
Infecções por Reoviridae/genética
Infecções por Reoviridae/virologia
Transdução de Sinais
Células Vero
Proteínas do Core Viral/genética
Proteínas Virais Reguladoras e Acessórias/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA-Binding Proteins); 0 (Viral Core Proteins); 0 (Viral Regulatory and Accessory Proteins); 0 (sigma NS protein, Reovirus); 0 (sigmaA protein, avian reovirus); EC 2.7.1.137 (Phosphatidylinositol 3-Kinase); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170127
[Lr] Data última revisão:
170127
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160529
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-016-2908-6


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[PMID]:27155492
[Au] Autor:Goldenberg D; Lublin A; Rosenbluth E; Heller ED; Pitcovski J
[Ad] Endereço:Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel; Migal - Galilee Technology Center, Kiryat Shmona, Israel.
[Ti] Título:Optimized polypeptide for a subunit vaccine against avian reovirus.
[So] Source:Vaccine;34(27):3178-3183, 2016 Jun 08.
[Is] ISSN:1873-2518
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Avian reovirus (ARV) is a disease-causing agent. The disease is prevented by vaccination with a genotype-specific vaccine while many variants of ARV exist in the field worldwide. Production of new attenuated vaccines is a long-term process and in the case of fast-mutating viruses, an impractical one. In the era of molecular biology, vaccines may be produced by using only the relevant protein for induction of neutralizing antibodies, enabling fast adjustment to the emergence of new genetic strains. Sigma C (SC) protein of ARV is a homotrimer that facilitates host-cell attachment and induce the production and secretion of neutralizing antibodies. The aim of this study was to identify the region of SC that will elicit a protective immune response. Full-length (residues 1-326) and two partial fragments of SC (residues 122-326 and 192-326) were produced in Escherichia coli. The SC fragment of residues 122-326 include the globular head, shaft and hinge domains, while eliminating intra-capsular region. This fragment induces significantly higher levels of anti-ARV antibodies than the shorter fragment or full length SC, which neutralized embryos infection by the virulent strain to a higher extent compared with the antibodies produced in response to the whole virus vaccine. Residues 122-326 fragment is assumed to be folded correctly, exposing linear as well as conformational epitopes that are identical to those of the native protein, while possibly excluding suppressor sequences. The results of this study may serve for the development of a recombinant subunit vaccine for ARV.
[Mh] Termos MeSH primário: Orthoreovirus Aviário
Doenças das Aves Domésticas/prevenção & controle
Infecções por Reoviridae/veterinária
Vacinas Virais/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Neutralizantes/sangue
Anticorpos Antivirais/sangue
Células Cultivadas
Galinhas/imunologia
Testes de Neutralização
Doenças das Aves Domésticas/virologia
Infecções por Reoviridae/prevenção & controle
Baço/citologia
Baço/imunologia
Vacinas de Subunidades/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Neutralizing); 0 (Antibodies, Viral); 0 (Vaccines, Subunit); 0 (Viral Vaccines)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160508
[St] Status:MEDLINE



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